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You searched for: Journal name Plant physiology Remove constraint Journal name: Plant physiology Publication year rev 8003-1997 Remove constraint Publication year rev: 8003-1997
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1. 7-Dehydrobrefeldin A, a naturally occurring brefeldin A derivative, inhibits secretion and causes a cis-to-trans breakdown of Golgi stacks in plant cells

Author:
Driouich, A.; Jauneau, A.; Staehelin, L.A.
Source:
Plant physiology 1997 v.113 no.2 pp. 487-492
ISSN:
0032-0889
Subject:
Carthamus tinctorius; Alternaria carthami; plant pathogenic fungi; phytotoxins; biosynthesis; hydrolysis; esterases; enzyme activity; phytotoxicity; bioassays; Acer pseudoplatanus; cell suspension culture; Golgi apparatus; Bacillus subtilis
Abstract:
... 7-Dehydrobrefeldin A (7-oxo-BFA) is a brefeldin A (BFA) analog that, like BFA, is a potent phytotoxin of Alternaria carthami, a fungal pathogen of safflower (Carthamus tinctorius L.) plants. Both BFA and 7-oxo-BFA have been shown to be causal agents of the leaf spot disease of these plants. We have investigated the effects of 7-oxo-BFA on the secretion and the structure of the Golgi stacks of syca ...
PubMed:
9046595
PubMed Central:
PMC158164

2. 8'-Methylene abscisic acid: an effective and persistent analog of abscisic acid

Author:
Abrams, S.R.; Rose, P.A.; Cutler, A.J.; Balsevich, J.J.; Lei, B.; Walker-Simmons, M.K.
Source:
Plant physiology 1997 v.114 no.1 pp. 89-97
ISSN:
0032-0889
Subject:
seed germination; Zea mays; chemical reactions; seedlings; cotyledons; biochemical pathways; carbon; transpiration; Triticum aestivum; growth retardation; gene expression; binding sites; abscisic acid; Lepidium sativum; synthesis; cell suspension culture
Abstract:
... We report here the synthesis and biological activity of a new persistent abscisic acid (ABA) analog, 8'-methylene ABA. This ABA analog has one additional carbon atom attached through a double bond to the 8'-carbon of the ABA molecule. (+)-8'-Methylene ABA is more active than the natural hormone (+)-ABA in inhibiting germination of cress seed and excised wheat embryos, in reducing growth of suspens ...

3. A 28-kilodalton pod storage protein of French bean plants. Purification, characterization, and primary structure

Author:
Zhong, P.Y.; Tanaka, T.; Yamauchi, D.; Minamikawa, T.
Source:
Plant physiology 1997 v.113 no.2 pp. 479-485
ISSN:
0032-0889
Subject:
Glycine max; protein synthesis; complementary DNA; genetic code; developmental stages; plant proteins; Phaseolus vulgaris; physicochemical properties; clones; amino acid sequences; ripening; nucleotide sequences; protein composition; pods
Abstract:
... When French bean (Phaseolus vulgaris) plants were depodded in the early stages of fruit development, relative levels of a specific protein with a relative molecular weight of 28,000 were enhanced in the young pods that formed later. The protein, designated pod storage protein (PSP), was purified from extracts of newly formed pods from plants that had been previously depodded four times at interval ...
PubMed:
9046594
PubMed Central:
PMC158163

4. A G-box-binding protein from soybean binds to the E1 auxin-response element in the soybean GH3 promoter and contains a proline-rich repression domain

Author:
Liu, Z.B.; Hagen, G.; Guilfoyle, T.J.
Source:
Plant physiology 1997 v.115 no.2 pp. 397-407
ISSN:
0032-0889
Subject:
protoplasts; gene transfer; Glycine max; leucine; genetic code; transgenic plants; Daucus carota; proline; luciferase; binding proteins; gene expression; plasmids; auxins; Nicotiana tabacum; transcription (genetics); reporter genes; enzyme activity; beta-glucuronidase
Abstract:
... The E1 promoter fragment (-249 to -203) is one of three auxin-response elements (AuxREs) in the soybean (Glycine max L.) GH3 promoter (Z.-B. Liu, T. Ulmasov, X. Shi, G. Hagen, T.J. Guilfoyle [1994] Plant Cell 6: 645-657). Results presented here further characterize and delimit the AuxRE within the E1 fragment. The E1 fragment functioned as an AuxRE in transgenic tobacco (Nicotiana tabacum L.) plan ...

5. A beta-amylase in potato tubers is induced by storage at low temperature

Author:
Nielsen, T.H.; Deiting, U.; Stitt, M.
Source:
Plant physiology 1997 v.113 no.2 pp. 503-510
ISSN:
0032-0889
Subject:
potatoes; postharvest physiology; Solanum tuberosum; tubers; food composition; starch; metabolism; hydrolysis; beta-amylase; enzyme activity; cold storage; metabolites; leaves; stems; temperature
Abstract:
... A new starch-degrading enzyme activity is induced by storage of potato (Solanum tuberosum L.) tubers at low temperatures (L. Hill, R. Reimholz, R. Schroder, T.H. Nielsen, M. Stitt [1996] Plant Cell Environ 14: 1223-1237). The cold-induced activity was separated from other amylolytic activities in zymograms based on iodine staining of polyacrylamide gels containing amylopectin. A similar band of ac ...

6. A cDNA clone highly expressed in ripe banana fruit shows homology to pectate lyases

Author:
Dominguez-Puigjaner, E.; Llop, I.; Vendrell, M.; Prat, S.
Source:
Plant physiology 1997 v.114 no.3 pp. 1071-1076
ISSN:
0032-0889
Subject:
Musa; complementary DNA; clones; messenger RNA; gene expression; genetic code; pectate lyase; protein synthesis; ripening; nucleotide sequences; amino acid sequences; transcription (genetics)
Abstract:
... A cDNA clone (BanI7), encoding a protein homologous to pectate lyase, has been isolated from a cDNA library from climacteric banana fruit by means of differential screening. Northern analysis showed that BanI7 mRNA is first detected in early climacteric fruit, reaches a steady-state maximum at the climacteric peak, and declines thereafter in overripe fruit. Accumulation of the BanI7 transcript can ...
PubMed:
9232883
PubMed Central:
PMC158396

7. A class IV chitinase is highly expressed in grape berries during ripening

Author:
Robinson, S.P.; Jacobs, A.K.; Dry, I.B.
Source:
Plant physiology 1997 v.114 no.3 pp. 771-778
ISSN:
0032-0889
Subject:
flowering; alleles; Phaseolus vulgaris; Brassica rapa subsp. oleifera; roots; sugars; clones; gene expression; Vitis vinifera; leaves; Zea mays; seeds; complementary DNA; beta-glucanase; amino acid sequences; enzyme activity; Beta vulgaris; biosynthesis; flowers; ripening; chitinase; South Australia
Abstract:
... Chitinase activity increased markedly at the onset of ripening in grape (Vitis vinifera L.) berries and continued to increase throughout the sugar accumulation phase of berry development. In contrast, beta-1,3-glucanase activity was not detected in grape berries at any stage of development. Two closely related chitinase cDNAs (VvChi4A and VvChi4B) were cloned from grapes. Sequence and Southern ana ...
PubMed:
9232868
PubMed Central:
PMC158363

8. A competitive enzyme-linked immunosorbent assay to quantify acetaldehyde-protein adducts that accumulate in dry seeds during aging

Author:
Zhang, M.; Nagata, S.; Miyazawa, K.; Kikuchi, H.; Esashi, Y.
Source:
Plant physiology 1997 v.113 no.2 pp. 397-402
ISSN:
0032-0889
Subject:
Xanthium strumarium; Lactuca sativa; Pisum sativum; Glycine max; Daucus carota; seeds; viability; protein content; acetaldehyde; biosynthesis; quantitative analysis; temperature; relative humidity; chemical reactions; biochemical pathways; enzyme-linked immunosorbent assay
Abstract:
... A competitive enzyme-linked immunosorbent assay (ELISA) was developed to quantify endogenous acetaldehyde-protein adducts (APAs) produced in plant seeds at low acetaldehyde concentrations without exogenous reducing agents. The key point of this technique is the use of a gelatin-acetaldehyde adduct, which is synthesized under 1 mM acetaldehyde and 10 mM NaCNBH3, to pre-coat plate wells to obtain th ...

9. A cysteine endopeptidase isolated from castor bean endosperm microbodies processes the glyoxysomal malate dehydrogenase precursor protein

Author:
Gietl, C.; Wimmer, B.; Adamex, J.; Kalousek, F.
Source:
Plant physiology 1997 v.113 no.3 pp. 863-871
ISSN:
0032-0889
Subject:
seed germination; Vigna mungo; proteinases; arginine; Ricinus communis; Phaseolus vulgaris; amino acid sequences; endosperm; Brassica napus; malate dehydrogenase; rats; enzyme activity; glyoxysomes; cysteine; protein composition; acyltransferases
Abstract:
... A plant cysteine endopeptidase with a molecular mass of 35 kD was purified from microbodies of germinating castor bean (Ricinus communis) endosperm by virtue of its capacity to specifically process the glyoxysomal malate dehydrogenase precursor protein to the mature subunit in vitro. Processing of the glyoxysomal malate dehydrogenase precursor occurs sequentially in three steps, the first intermed ...
PubMed:
9085576
PubMed Central:
PMC158206

10. A defective signal peptide tethers the floury-2 zein to the endoplasmic reticulum membrane

Author:
Gillikin, J.W.; Zhang, F.; Coleman, C.E.; Bass, H.W.; Larkins, B.A.; Boston, R.S.
Source:
Plant physiology 1997 v.114 no.1 pp. 345-352
ISSN:
0032-0889
Subject:
Zea mays; microsomes; zein; binding proteins; endoplasmic reticulum; protein synthesis; mutagenesis; RNA; protein transport; mutants; genetic variation; transcription (genetics)
Abstract:
... The maize (Zea mays L.) floury-2 (fl2) mutation is associated with a general decrease in storage protein synthesis, altered protein body morphology, and the synthesis of a novel 24-kD alpha-zein storage protein. Unlike storage proteins in normal kernels and the majority of storage proteins in fl2 kernels, the 24-kD alpha-zein contains a signal peptide that would normally be removed during protein ...
PubMed:
9159955
PubMed Central:
PMC158310

11. A dynamin-like protein, ADL1, is present in membranes as a high-molecular-mass complex in Arabidopsis thaliana

Author:
Park, J.M.; Kang, S.G.; Pih, K.T.; Jang, H.J.; Piao, H.L.; Yoon, H.W.; Cho, M.J.; Hwang, I.
Source:
Plant physiology 1997 v.115 no.2 pp. 763-771
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; protein composition; calcium; guanosine triphosphate; flowers; cytosol; binding proteins; physicochemical properties; protein transport; cell membranes; adenosine triphosphate; roots; leaves; cyclosporins
Abstract:
... Dynamin, a GTP-binding protein, is involved in endocytosis in animal cells. We found that a dynamin-like protein, ADL1, is present in multiple forms in Arabidopsis leaf tissue. Subcellular fractionation experiments, together with gel-filtration and nondenaturing-gel electrophoresis revealed that most of ADL1 is present as a high-molecular-mass complex of 400 to 600 kD in the membrane or pellet fra ...
PubMed:
9342876
PubMed Central:
PMC158536

12. A gene encoding a chloroplast-targeted lipoxygenase in tomato leaves is transiently induced by wounding, systemin, and methyl jasmonate

Author:
Heitz, T.; Bergey, D.R.; Ryan, C.A.
Source:
Plant physiology 1997 v.114 no.3 pp. 1085-1093
ISSN:
0032-0889
Subject:
messenger RNA; protein composition; protein synthesis; complementary DNA; genetic code; jasmonic acid; linoleate 13S-lipoxygenase; defense mechanisms; stearic acid; amino acid sequences; gene expression; biochemical pathways; protein transport; leaves; genes; ripening; chloroplasts; Solanum lycopersicum var. lycopersicum
Abstract:
... We investigated the relationship between the expression of lipoxygenase (LOX) genes and the systemin-dependent wound response in tomato (Lycopersicon esculentum) leaves. A polymerase chain reaction-based approach was used to isolate two tomato Lox cDNAs, called TomLoxC and TomLoxD. Both TomLOXC and TomLOXD amino acid sequences possess an N-terminal extension of about 60 residues that were shown by ...
PubMed:
9232884
PubMed Central:
PMC158398

13. A new mechanism for the regulation of stomatal aperture size in intact leaves: accumulation of mesophyll-derived sucrose in the guard-cell wall of Vicia faba

Author:
Lu, P.; Outlaw, W.H. Jr.; Smith, B.G.; Freed, G.A.
Source:
Plant physiology 1997 v.114 no.1 pp. 109-118
ISSN:
0032-0889
Subject:
Vicia faba; mesophyll; stomata; guard cells; sucrose; quantitative analysis; histochemistry; transpiration; photosynthesis; phloem loading; radiolabeling; cell wall components
Abstract:
... At various times after pulse-labeling broad bean (Vicia faba L.) leaflets with 14CO2, whole-leaf pieces and rinsed epidermal peels were harvested and subsequently processed for histochemicalanalysis. Cells dissected from whole leaf retained apoplastic contents, whereas those from rinsed peels contained only symplastic contents. Sucrose (Suc)-specific radioactivity peaked (111 GBq mol-1) in palisad ...

14. A nitrate-inducible ferredoxin in maize roots. Genomic organization and differential expression of two nonphotosynthetic ferredoxin isoproteins

Author:
Matsumura, T.; Sakakibara, H.; Nakano, R.; Kimata, Y.; Sugiyama, T.; Hase, T.
Source:
Plant physiology 1997 v.114 no.2 pp. 653-660
ISSN:
0032-0889
Subject:
Zea mays; biosynthesis; restriction mapping; complementary DNA; seedlings; amino acid sequences; gene expression; genes; nucleotide sequences; genomics; chemical constituents of plants; roots; nitrates
Abstract:
... We have identified and characterized a nitrate-inducible ferredoxin (Fd) in maize (Zea mays L.) roots by structural analysis of the purified protein and by cloning of its cDNA and gene. In maize Fd isoproteins are encoded by a small multigene family, and the nitrate-inducible Fd was identified as a novel isoprotein, designated Fd VI, which differed from any Fd I to Fd V identified to date. In the ...
PubMed:
9193097
PubMed Central:
PMC158349

15. A plant chloroplast glutamyl proteinase

Author:
Laing, W.A.; Christeller, J.T.
Source:
Plant physiology 1997 v.114 no.2 pp. 715-722
ISSN:
0032-0889
Subject:
protein composition; glutamine; proteinases; Spinacia oleracea; physicochemical properties; casein; enzyme activity; chloroplasts
Abstract:
... A glutamyl proteinase was partially purified from Percoll gradient-purified spinach (Spinacia oleracea) chloroplast preparations and appeared to be predominantly localized in the chloroplast stroma. The enzyme degraded casein, but of the 11 synthetic endopeptidase substrates tested, only benzyloxycarbonyl-leucine-leucine-glutamic acid-beta-napthylamide was hydrolyzed at measurable rates. In additi ...

16. A radial concentration gradient of indole-3-acetic acid is related to secondary xylem development in hybrid aspen

Author:
Tuominen, H.; Puech, L.; Fink, S.; Sundberg, B.
Source:
Plant physiology 1997 v.115 no.2 pp. 577-585
ISSN:
0032-0889
Subject:
gene transfer; plant development; hybrids; biosynthesis; dose response; stems; morphogenesis; cell growth; developmental stages; transgenic plants; genetic variation; Agrobacterium radiobacter; cell division; gene expression; Populus tremuloides; Populus tremula; cambium; xylem; indole acetic acid
Abstract:
... The radial distribution pattern of indole-3-acetic acid (IAA) was determined across the developing tissues of the cambial region in the stem of hybrid aspen (Populus tremula L. x Populus tremuloides Michx). IAA content was measured in consecutive tangential cryosections using a microscale mass spectrometry technique. Analysis was performed with wild-type and transgenic trees with an ectopic expres ...

17. A secreted factor induces cell expansion and formation of metaxylem-like tracheary elements in xylogenic suspension cultures of Zinnia

Author:
Roberts, A.W.; Donovan, S.G.; Haigler, C.H.
Source:
Plant physiology 1997 v.115 no.2 pp. 683-692
ISSN:
0032-0889
Subject:
plant secretions and exudates; tracheids; cell growth; mesophyll; Zinnia violacea; physicochemical properties; cell differentiation; oligosaccharides; cell suspension culture
Abstract:
... Conditioned medium from mesophyll cell-suspension cultures of Zinnia elegans L. has striking effects on cell expansion and tracheary element differentiation when applied to cultures of freshly isolated mesophyll cells. These effects include (a) induction of early cell expansion, (b) delay in differentiation by 48 h or more, (c) reduction in the synchrony of differentiation, and (d) early formation ...

18. A single-seed assay for endo-beta-mannanase activity from tomato endosperm and radicle tissues

Author:
Still, D.W.; Dahal, P.; Bradford, K.J.
Source:
Plant physiology 1997 v.113 no.1 pp. 13-20
ISSN:
0032-0889
Subject:
Solanum lycopersicum var. lycopersicum; seed germination; endosperm; developmental stages; protein composition; mannosidases; enzyme activity; quantitative analysis; assays
Abstract:
... Completion of germination (radicle emergence) is an all-or-none developmental event for an individual seed. Variation in germination timing among seeds in a population therefore reflects variation among seeds in the rates or extents of physiological or biochemical processes prior to radicle emergence. For tomato (Lycopersicon esculentum Mill.) seeds, correlative evidence suggests that endo-beta-ma ...

19. A ubiquitous plant housekeeping gene, PAP, encodes a major protein component of bell pepper chromoplasts

Author:
Pozueta-Romero, J.; Rafia, F.; Houlne, G.; Cheniclet, C.; Carde, J.P.; Schantz, M.L.; Schantz, R.
Source:
Plant physiology 1997 v.115 no.3 pp. 1185-1194
ISSN:
0032-0889
Subject:
complementary DNA; Capsicum annuum; genetic code; developmental stages; ontogeny; lipids; gene expression; amino acid sequences; plastids; genes; ripening; transcription (genetics); chemical constituents of plants; cell differentiation; immunocytochemistry; leaves; plant proteins
Abstract:
... We have isolated a cDNA (PAP) corresponding to a single nuclear gene that encodes an approximately 30-kD major protein of bell pepper (Capsicum annuum L.) fruit chromoplasts. RNA and protein analyses revealed that, although at a low level, this gene is also expressed in every organ of the plant, the amount of the corresponding transcript and protein dramatically increasing in the latter stages of ...
PubMed:
9390444
PubMed Central:
PMC158583

20. A vacuole-associated annexin protein, VCaB42, correlates with the expansion of tobacco cells

Author:
Seals, D.F.; Randall, S.K.
Source:
Plant physiology 1997 v.115 no.2 pp. 753-761
ISSN:
0032-0889
Subject:
protoplasts; morphogenesis; vacuoles; calnexin; Nicotiana tabacum; cell membranes; adenosinetriphosphatase; endoplasmic reticulum; cell suspension culture; protein composition
Abstract:
... A Ca-dependent membrane-binding protein of the annexin family, VCaB42, has previously been shown to associate with vacuolar vesicles at physiological levels of Ca. In this study we used suspension-cultured cells of tobacco (Nicotiana tabacum BY-2) to show that VCaB42 is enriched 4.5-fold in intact vacuoles, whereas evacuolated protoplasts show a 12-fold reduction in VCaB42. VCaB42 distribution is ...

21. A water-soluble chlorophyll protein in cauliflower may be identical to BnD22, a drought-induced, 22-kilodalton protein in rapeseed

Author:
Nishio, N.; Satoh, H.
Source:
Plant physiology 1997 v.115 no.2 pp. 841-846
ISSN:
0032-0889
Subject:
protein synthesis; Glycine max; stress response; drought; binding proteins; amino acid sequences; leaves; Brassica oleracea; Brassica napus; solubility; chlorophyll; protein composition; Solanum lycopersicum var. lycopersicum
Abstract:
... A water-soluble chlorophyll protein (WSCP) in cauliflower (Brassica oleracea L.) was purified and its N-terminal sequence was determined. Forty-six of 48residues of the sequence completely matched those of the drought-induced 22-kD protein (BnD22) in rapeseed (Brassica napus L.). All 40 sequenced residues of WSCP from rapeseed were perfectly matched to those of BnD22. Thus, WSCP may be identical t ...
PubMed:
9342880
PubMed Central:
PMC158544

22. A xyloglucan-specific endo-1,4-beta-glucanase isolated from auxin-treated pea stems

Author:
Matsumoto, T.; Sakai, F.; Hayashi, T.
Source:
Plant physiology 1997 v.114 no.2 pp. 661-667
ISSN:
0032-0889
Subject:
metabolism; stems; xyloglucans; cellulases; beta-glucanase; auxins; chemical constituents of plants; Pisum sativum; protein content; enzyme activity
Abstract:
... A xyloglucan-specific endo-1,4-beta-glucanase was isolated from the apoplast fraction of auxin-treated pea (Pisum sativum) stems, in which both the rate of stem elongation and the amount of xyloglucan solubilized were high. The enzyme was purified to apparent homogeneity by sequential cation-exchange chromatographies, affinity chromatography, and gel filtration. The purified enzyme gave a single p ...

23. ATP hydrolysis activity and polymerization state of ribulose-1,5-bisphosphate carboxylase oxygenase activase. Do the effects of Mg2+, K+, and activase concentrations indicate a functional similarity to actin?

Author:
Lilley, R.M.; Portis, A.R. Jr.
Source:
Plant physiology 1997 v.114 no.2 pp. 605-613
ISSN:
0032-0889
Subject:
ribulose-bisphosphate carboxylase; dose response; polymerization; Spinacia oleracea; adenosine triphosphate; magnesium; biochemical pathways; anions; potassium; hydrolysis; actin; enzyme activity; adenosinetriphosphatase
Abstract:
... The ATPase activity and fluorescence of ribulose-1,5-bisphosphate carboxylase oxygenase (Rubisco) activase were determined over a range of MgCl2, KCl, and activase concentrations. Both salts promoted ADP release from ATP and intrinsic fluorescence enhancement by adenosine 5'-[gamma-thio] triphosphate, but Mg2+ was about 10 times more effective than K+. ATPase and fluorescence enhancement both incr ...

24. Abolition of posttranscriptional regulation of nitrate reductase partially prevents the decrease in leaf NO3(-) reduction when photosynthesis is inhibited by CO2 deprivation, but not in darkness

Author:
Lejay, L.; Quillere, I.; Roux, Y.R.; Tillard, P.; Cliquet, J.B.; Meyer, C.; Morot-Gaudry, J.F.; Gojon, A.
Source:
Plant physiology 1997 v.115 no.2 pp. 623-631
ISSN:
0032-0889
Subject:
Nicotiana plumbaginifolia; leaves; nitrate reduction; nitrate reductase; enzyme activity; light; photosynthesis; roots; free amino acids; transgenic plants; complementary DNA; gene expression; genetic variation; transcription (genetics); chemical constituents of plants; genotype
Abstract:
... The activity of nitrate reductase (NR) in leaves is regulated by light and photosynthesis at transcriptional and posttranscriptional levels. To understand the physiological role of these controls, we have investigated the effects of light and CO2 on in vivo NO3- reduction in transgenic plants of Nicotiana plumbaginifolia lacking either transcriptional regulation alone or transcriptional and post-t ...

25. Abscisic acid activates a 48-kilodalton protein kinase in guard cell protoplasts

Author:
Mori, I.C.; Muto, S.
Source:
Plant physiology 1997 v.113 no.3 pp. 833-839
ISSN:
0032-0889
Subject:
Vicia faba; guard cells; protoplasts; protein composition; enzyme activity; abscisic acid; enzyme inhibitors; kinetics; binding proteins; phosphorylation; stomatal movement; myelin sheath
Abstract:
... A 49- and a 46-kD Ca2+-independent protein kinase and a 53-kD Ca2+-dependent protein kinase were detected in Vicia faba guard cell protoplasts (GCPs) by an in-gel protein kinase assay using myelin basic protein as a substrate. A 48-kD protein kinase designated as abscisicacid (ABA)-responsive protein kinase (ABR kinase) appeared when GCPs were treated with ABA. The activation of ABR kinase was sup ...

26. Abundant accumulation of the calcium-binding molecular chaperone calreticulin in specific floral tissues of Arabidopsis thaliana

Author:
Nelson, D.E.; Glaunsinger, B.; Bohnert, H.J.
Source:
Plant physiology 1997 v.114 no.1 pp. 29-37
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; ovules; protein synthesis; quantitative analysis; anthers; genetic code; reticulin; developmental stages; nectaries; transcription (genetics); gene expression; endosperm; genes; calcium-binding proteins; immunocytochemistry; endoplasmic reticulum
Abstract:
... Calreticulin (CRT) is a calcium-binding protein in the endoplasmic reticulum (ER) with an established role as a molecular chaperone. An additional function in signal transduction, specifically in calcium distribution, is suggested but not proven. We have analyzed the expression pattern of Arabidopsis thaliana CRTs for a comparison with these proposed roles. Three CRT genes were expressed, with ide ...
PubMed:
9159940
PubMed Central:
PMC158275

27. Accelerated early growth of rice at elevated CO2. Is it related to developmental changes in the shoot apex?

Author:
Jitla, D.S.; Rogers, G.S.; Seneweera, S.P.; Basra, A.S.; Oldfield, R.J.; Conroy, J.P.
Source:
Plant physiology 1997 v.115 no.1 pp. 15-22
ISSN:
0032-0889
Subject:
plant development; buds; diameter; dose response; carbon dioxide; Oryza sativa; developmental stages; internodes; crop yield; length; tillers; leaves
Abstract:
... The influence of elevated CO2 on the development of the shoot apex and on subsequent vegetative growth and grain yield investigated using rice (Oryza sativa L. cv Jarrah) grown in flooded soil at either 350 or 700 microliter CO2 L-1. At 8 d after planting (DAP), elevated CO2 increased the height and diameter of the apical dome and lengths of leaf primordia and tiller buds but had no effect on thei ...

28. Accumulation of ricinoleic, lesquerolic, and densipolic acids in seeds of transgenic arabidopsis plants that express a fatty acyl hydroxylase cDNA from castor bean

Author:
Broun, P.; Somerville, C.
Source:
Plant physiology 1997 v.113 no.3 pp. 933-942
ISSN:
0032-0889
Subject:
Physaria fendleri; genes; Brassica napus; oleic acid; promoter regions; Ricinus communis; gene expression; Lesquerella; genotype; ricinoleic acid; oxygenases; Arabidopsis thaliana; messenger RNA; seeds; quantitative analysis; complementary DNA; transcription (genetics); enzyme activity; gene transfer; biosynthesis; gadoleic acid; transgenic plants; biochemical pathways; genetic variation
Abstract:
... A cDNA encoding the oleate 12-hydroxylase from castor bean (Ricinus communis L.) has previously been shown to direct the synthesis of small amounts of ricinoleic acid (12-hydroxyoctadec-cis-9-enoic acid) in seeds of transgenic tobacco plants. Expression of the cDNA under control of the Brassica napus napin promoter in transgenic Arabidopsis thaliana plants resulted in the accumulation of up to 17% ...
PubMed:
9085577
PubMed Central:
PMC158213

29. Accumulation of zeaxanthin in abscisic acid-deficient mutants of Arabidopsis does not affect chlorophyll fluorescence quenching or sensitivity to photoinhibition in vivo

Author:
Hurry, V.; Anderson, J.M.; Chow, W.S.; Osmond, C.B.
Source:
Plant physiology 1997 v.113 no.2 pp. 639-648
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; biosynthesis; quantitative analysis; fluorescence; light harvesting complex; genetic variation; neoxanthin; photoinhibition; zeaxanthin; photosystem II; mutants; photosystem I; abscisic acid; measurement; chlorophyll; violaxanthin; leaves
Abstract:
... Abscisic acid (ABA)-deficient mutants of Arabidopsis do not synthesize the epoxy-xanthophylls antheraxanthin, violaxanthin, or neoxanthin. However, thylakoid membranes from these mutants contain 3-fold more zeaxanthin than wild-type plants. This increase in zeaxanthin occurs as a stoichiometric replacement of the missing violaxanthin and neoxanthin within the pigment-protein complexes of both phot ...

30. Acetaldehyde is a causal agent responsible for ethanol-induced ripening inhibition in tomato fruit

Author:
Beaulieu, J.C.; Peiser, G.; Saltveit, M.E.
Source:
Plant physiology 1997 v.113 no.2 pp. 431-439
ISSN:
0032-0889
Subject:
temperature; Solanum lycopersicum var. lycopersicum; ripening; ethanol; dose response; acetaldehyde; biosynthesis; quantitative analysis; alcohol dehydrogenase; aldehyde dehydrogenase; enzyme inhibitors
Abstract:
... Inhibition of tomato (Lycopersicon esculentum Mill.) fruit ripening by exogenously applied ethanol was shown to be caused by elevated endogenous levels of acetaldehyde (AA). Exposure of excised pericarp discs of mature-green tomato fruit to ethanol or AA vapors produced elevated levels of both compounds in the tissue, but only the levels of AA were associated with ripening inhibition. Ripening inh ...

31. Acidic phosphoprotein complex of the 60S ribosomal subunit of maize seedling roots. Components and changes in response to flooding

Author:
Bailey-Serres, J.; Vangala, S.; Szick, K.; Lee, C.H.K.
Source:
Plant physiology 1997 v.114 no.4 pp. 1293-1305
ISSN:
0032-0889
Subject:
Zea mays; protein synthesis; messenger RNA; complementary DNA; phosphoproteins; seedlings; stress response; Saccharomyces cerevisiae; water stress; ribosomes; amino acid sequences; genes; nucleotide sequences; phosphotransferases (kinases); phosphorylation; enzyme activity; roots; protein composition
Abstract:
... We determined that ribosomes of seedling roots of maize (Zea mays L.) contain the acidic phosphoproteins (P-proteins) known to form a flexible lateral stalk structure of the 60S subunit of eukaryotic ribosomes. The P-protein stalk, composed of P0, P1, and P2, interacts with elongation factors, mRNA, and tRNA during translation. Acidic proteins of 13 to 15.5 kD were released as a complex from ribos ...
PubMed:
9276949
PubMed Central:
PMC158422

32. Actin filaments modulate both stomatal opening and inward K+ -channel activities in guard cells of Vicia faba L

Author:
Hwang, J.U.; Suh, S.; Yi, H.; Kim, J.; Lee, Y.
Source:
Plant physiology 1997 v.115 no.2 pp. 335-342
ISSN:
0032-0889
Subject:
Vicia faba; stomatal movement; guard cells; protoplasts; volume; potassium; ion transport; actin; polymerization; metabolic inhibitors; electric current; membrane potential; measurement; cytochalasin D
Abstract:
... Actin antagonists have previously been shown to alter responses of Commelina communis stomata to physiological stimuli, implicating actin filaments in the control of guard cell volume changes (M. Kim, P.K. Hepler, S.-O. Eun, K.S. Ha, Y. Lee [1995] Plant Physiol 109:1077-1084). Since K+ channels in the guard cell play an important role in stomatal movements, we examined the possible regulation of K ...

33. Actin filaments of guard cells are reorganized in response to light and abscisic acid

Author:
Eun, S.O.; Lee, Y.
Source:
Plant physiology 1997 v.115 no.4 pp. 1491-1498
ISSN:
0032-0889
Subject:
guard cells; stomatal movement; cortex; chemical constituents of plants; Commelina communis; developmental stages; actin; light; abscisic acid; leaves
Abstract:
... We recently showed that treatment with actin antagonists perturbed stomatal behavior in Commelina communis L. leaf epidermis and therefore suggested that dynamic changes in actin are necessary for signal responses in guard cells (M. Kim, P.K. Hepler, S.O. Eun, K.-S. Ha, Y. Lee [1995] Plant Physiol 109:1077-1084). Here we show that actin filaments of guard cells, visualized by immunofluorescence mi ...
PubMed:
9414559
PubMed Central:
PMC158614

34. Activation of host defense mechanisms by elevated production of H2O2 in transgenic plants

Author:
Wu, G.; Shortt, B.J.; Lawrence, E.B.; Leon, J.; Fitzsimmons, K.C.; Levine, E.B.; Raskin, I.; Shah, D.M.
Source:
Plant physiology 1997 v.115 no.2 pp. 427-435
ISSN:
0032-0889
Subject:
Solanum tuberosum; Verticillium dahliae; plant pathogenic fungi; leaves; pathogenicity; disease resistance; hydrogen peroxide; biosynthesis; salicylic acid; quantitative analysis; gene expression; transgenic plants; messenger RNA; genetic code; glucose oxidase; peroxidases; chitinase; isozymes; enzyme activity; stems; roots; lignin; genetic variation; chemical constituents of plants; genotype
Abstract:
... Active oxygen specieshave been postulated to perform multiple functions in plant defense, but their exact role in plant resistance to diseases is not fully understood. We have recently demonstrated H2O2-mediated disease resistance in transgenic potato (Solanum tuberosum) plants expressing a foreign gene encoding glucose oxidase. In this study we provide further evidence that the H2O2-mediated dise ...

35. Activation of plant plasma membrane Ca2+-permeable channels by race-specific fungal elicitors

Author:
Gelli, A.; Higgins, V.J.; Blumwald, E.
Source:
Plant physiology 1997 v.113 no.1 pp. 269-279
ISSN:
0032-0889
Subject:
Solanum lycopersicum var. lycopersicum; calcium; defense mechanisms; ion transport; plant pathogenic fungi; disease resistance; plasma membrane; phosphorylation; enzyme activity; cell suspension culture; leaves; Passalora fulva
Abstract:
... The response of plant cells to invading pathogens is regulated by fluctuations in cytosolic Ca2+ levels that are mediated by Ca2+-permeable channels located at the plasma membrane of the host cell. The mechanisms by which fungal elicitors can induce Ca2+ uptake by the host cell were examined by the application of conventional patch-clamp techniques. Whole-cell and single-channel experiments on tom ...

36. Activation of pollen tube callose synthase by detergents. Evidence for different mechanisms of action

Author:
Li, H.J.; Bacic, A.; Read, S.M.
Source:
Plant physiology 1997 v.114 no.4 pp. 1255-1265
ISSN:
0032-0889
Subject:
Nicotiana alata; pollen tubes; callose; biosynthesis; hexosyltransferases; detergents; digitonin; dose response; pyridoxal; sulfonates; phosphatidylcholines; trypsin; cell wall components; beta-glucans
Abstract:
... In pollen tubes of Nicotiana alata, a membrane-bound, Ca2+-independent callose synthase (CalS) is responsible for the biosynthesis of the (1,3)-beta-glucan backbone of callose, the main cell wall component. Digitonin increases CalS activity 3- to 4-fold over a wide range of concentrations, increasing the maximum initial velocity without altering the Michaelis constant for UDP-glucose. The CalS act ...
PubMed:
9276948
PubMed Central:
PMC158418

37. Adenosine 5'-triphosphate is required for the assembly of 11A seed proglobulins in vitro

Author:
Nam, Y.W.; Jung, R.; Nielsen, N.C.
Source:
Plant physiology 1997 v.115 no.4 pp. 1629-1639
ISSN:
0032-0889
Subject:
Glycine max; Vicia faba; seeds; globulins; protein synthesis; adenosine triphosphate; binding sites
Abstract:
... Seed protein proglobulins were synthesized from cDNAs in reticulocyte lysates. Most proglobulins were recovered as trimers when translation rates were low, but mostly monomers were recovered at high translation rates. The prevalence of monomers was accompanied by elevated amounts of insoluble protein recovered at the bottom of sucrose density gradients. Apyrase treatment of translation mixtures af ...

38. Adenosine-5'-phosphate deaminase: a novel herbicide target

Author:
Dancer, J.E.; Hughes, R.G.; Lindell, S.D.
Source:
Plant physiology 1997 v.114 no.1 pp. 119-129
ISSN:
0032-0889
Subject:
herbicidal properties; adenosine deaminase; weed control; metabolism; herbicides; Persicaria lapathifolia; adenosine monophosphate; enzyme inhibitors; structure-activity relationships; phosphorylation; Pisum sativum; enzyme activity
Abstract:
... The isolation of carbocyclic coformycin as the herbicidally active component from a fermentation of Saccharothrix species was described previously (B.D. Bush, G.V. Fitchett, D.A. Gates, D. Langley [1993] Phytochemistry 32:737-739). Here we report that the primary mode of action of carbocyclic coformycin has been identified as inhibition of the enzyme AMP deaminase (EC 3.5.4.6) following phosphoryl ...
PubMed:
9159944
PubMed Central:
PMC158285

39. Adenylosuccinate synthetase from maize. Purification, properties, and mechanism of inhibition by 5'-phosphohydantocidin

Author:
Walters, E.W.; Lee, S.F.; Niderman, T.; Bernasconi, P.; Subramanian, M.V.; Siehl, D.L.
Source:
Plant physiology 1997 v.114 no.2 pp. 549-555
ISSN:
0032-0889
Subject:
Zea mays; succinic acid; enzyme inhibitors; ligases; pH; kinetics; enzyme activity; chemical structure; protein composition
Abstract:
... Adenylosuccinate synthetase (AdSS) is the site of action of hydantocidin, a potent microbial phytotoxin. A kinetic analysis of the mode of inhibition of a plant adenylosuccinate synthetase by the active metabolite 5'-phosphohydantocidin (5'-PH) was the objective of the present study. AdSS was purified 5800-fold from maize (Zea mays), to our knowledge the first purification of the enzyme from a pla ...
PubMed:
9193088
PubMed Central:
PMC158335

40. Al inhibits both shoot development and root growth in als3, an Al-sensitive Arabidopsis mutant

Author:
Larsen, P.B.; Kochian, L.V.; Howell, S.H.
Source:
Plant physiology 1997 v.114 no.4 pp. 1207-1214
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; plant development; biosynthesis; phenotype; callose; phytotoxicity; stress response; aluminum; mutants; genotype; genetic variation; ultrastructure; plant morphology; roots; leaves; shoots
Abstract:
... In als3, an Al-sensitive Arabidopsis mutant, shoot development and root growth are sensitive to Al. Mutant als3 seedlings grown in an Al-containing medium exhibit severely inhibited leaf expansion and root growth. In the presence of Al, unexpanded leaves accumulate callose, an indicator of Al damage in roots. The possibility that the inhibition of shoot development in als3 is due to the hyperaccum ...

41. Al partitioning patterns and root growth as related to Al sensitivity and Al tolerance in wheat

Author:
Samuels, T.D.; Kucukakyuz, K.; Rincon-Zachary, M.
Source:
Plant physiology 1997 v.113 no.2 pp. 527-534
ISSN:
0032-0889
Subject:
Triticum aestivum; roots; aluminum; quantitative analysis; dose response; phytotoxicity; cultivars; translocation (plant physiology); biological resistance
Abstract:
... Studies of Al partitioning and accumulation and of the effect of Al on the growth of intact wheat (Triticum aestivum L.) roots of cultivars that show differential Al sensitivity were conducted. The effects of various Al concentrations on root growth and Al accumulation inthe tissue were followed for 24 h. At low external Al concentrations, Al accumulation in the root tips was low and root growth w ...

42. Al-induced, 51-kilodalton, membrane-bound proteins are associated with resistance to Al in a segregating population of wheat

Author:
Taylor, G.J.; Basu, A.; Basu, U.; Slaski, J.J.; Zhang, G.; Good, A.
Source:
Plant physiology 1997 v.114 no.1 pp. 363-372
ISSN:
0032-0889
Subject:
cultivars; sulfur; protein synthesis; dose response; quantitative analysis; callose; phytotoxicity; radiolabeling; stress response; cytosol; microsomes; Triticum aestivum; aluminum; mitochondria; tonoplast; genetic variation; plasma membrane; genotype; protein composition
Abstract:
... Incorporation of 35S into protein is reduced by exposure to Al in wheat (Triticum aestivum), but the effects are genotype-specific. Exposure to 10 to 75 micromolars Al had little effect on 35S incorporation into total protein, nuclear and mitochondrial protein, microsomal protein, and cytosolic protein in the Al-resistant cultivar PT741. In contrast, 10 micromolars Al reduced incorporation by 21 t ...

43. Allocation of S in generative growth of soybean

Author:
Sunarpi; Anderson, J.W.
Source:
Plant physiology 1997 v.114 no.2 pp. 687-693
ISSN:
0032-0889
Subject:
Glycine max; leaves; pods; seeds; sulfur; sulfates; nutrient transport; nitrogen; glutathione; cysteine; methionine; seed development; developmental stages; chemical constituents of plants
Abstract:
... Soybean plants (Glycine max L. Merr) were grown with 100 micromolar S and 15 mM N and studied with respect to S allocation during grain development. The grains accounted for 87% of the S taken up after d 42, the balance coming from internal redistribution of S from leaves and pods. Detailed studies of the leaves, pods, and grains associated with leaf axils 6 and 7 showed that sulfate accumulated i ...

44. Amino acid utilization in seeds of loblolly pine during germination and early seedling growth. I. Arginine and arginase activity

Author:
King, J.E.; Gifford, D.J.
Source:
Plant physiology 1997 v.113 no.4 pp. 1125-1135
ISSN:
0032-0889
Subject:
Pinus taeda; seed germination; seedling growth; seeds; arginine; metabolism; arginase; enzyme activity; protein degradation; metabolites; free amino acids; developmental stages; quantitative analysis
Abstract:
... The mobilization and utilization of the major storage proteins in loblolly pine (Pinus taeda L.) seeds following imbibition were investigated. Most of the seed protein reserves were contained within the megagametophyte. Breakdown of these proteins occurred primarily following radicle emergence and correlated with a substantial increase in the free amino acid pool in the seedling; the majority of t ...

45. An electron paramagnetic resonance spin-probe study of membrane-permeability changes with seed aging

Author:
Golovina, E.A.; Tikhonov, A.N.; Hoekstra, F.A.
Source:
Plant physiology 1997 v.114 no.1 pp. 383-389
ISSN:
0032-0889
Subject:
Triticum aestivum; wheat; membrane permeability; measurement; electron paramagnetic resonance spectroscopy; seed germination; viability; shelf life; postharvest physiology; germplasm; plasma membrane
Abstract:
... We developed an electron paramagnetic resonance spin-probe technique to study changes in the barrier properties of plasma membranes in wheat (Triticum aestivum L.) seeds during aging under dry storage. The estimation of these barrier properties was based on the differential permeability of membranes for the stable free radical 4-oxo-2,2,6,6-tetramethyl-1-piperidinyloxy and the broadening agent fer ...

46. Analysis of cDNA clones encoding sucrose-phosphate synthase in relation to sugar interconversions associated with dehydration in the resurrection plant Craterostigma plantagineum Hochst

Author:
Ingram, J.; Chandler, J.W.; Gallagher, L.; Salamini, F.; Bartels, D.
Source:
Plant physiology 1997 v.115 no.1 pp. 113-121
ISSN:
0032-0889
Subject:
rehydration; biosynthesis; complementary DNA; genetic code; glucose; sucrose; stress response; fructose 6-phosphate; clones; gene expression; amino acid sequences; transcription (genetics); cytoplasm; phosphates; Scrophulariaceae; enzyme activity; sucrose synthase; roots; leaves
Abstract:
... Sucrose-phosphate synthase (SPS) is a key enzyme in the regulation of sucrose metabolism, being responsible for the synthesis of sucrose 6-phosphate from fructose 6-phosphate and uridine 5'-diphosphate-glucose. We report on the isolation and characterization of cDNA clones encoding SPS from Craterostigma plantagineum Hochst., a resurrection plant in which the accumulation of sucrose is considered ...
PubMed:
9306694
PubMed Central:
PMC158466

47. Analysis of wild-type and mutant plant nitrate reductase expressed in the methylotrophic yeast Pichia pastoris

Author:
Su, W.P.; Mertens, J.A.; Kanamaru, K.; Campbell, W.H.; Crawford, N.M.
Source:
Plant physiology 1997 v.115 no.3 pp. 1135-1143
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; serine; nitrate reductase; recombinant proteins; mutagenesis; kinetics; alanine; adenosine triphosphate; gene expression; genes; genetic engineering; mutants; genetic variation; enzyme activity; cysteine; Pichia
Abstract:
... Recombinant Arabidopsis thaliana NADH:nitrate reductase (NR; EC 1.6.6.1) was produced in the methylotrophic yeast Pichia pastoris and purified to near-electrophoretic homogeneity. Purified enzyme had the spectral and kinetic properties typical of highly purified NR from natural plant sources. Site-directed mutagenesis altering several key residues and regions was carried out, and the mutant enzyme ...
PubMed:
9390442
PubMed Central:
PMC158578

48. Anion-channel blockers interfere with auxin responses in dark-grown Arabidopsis hypocotyls

Author:
Thomine, S.; Lelievre, F.; Boufflet, M.; Guern, J.; Barbier-Brygoo, H.
Source:
Plant physiology 1997 v.115 no.2 pp. 533-542
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; acetic acid; turgor; hypocotyls; developmental stages; ion transport; auxins; anions; etiolation; sulfonates; plant morphology
Abstract:
... Anion channels are thought to participate in signal transduction and turgor regulation in higher plant cells. The regulation of hypocotyl cell elongation is a situation in which these channels could play important roles because it involves ionic fluxes that are implicated in turgor control and orchestrated by various signals. We have used a pharmacological approach to reveal the contribution of an ...
PubMed:
9342869
PubMed Central:
PMC158512

49. Antagonistic but complementary actions of phytochromes A and B allow optimum seedling de-etiolation

Author:
Smith, H.; Xu, Y.; Quail, P.H.
Source:
Plant physiology 1997 v.114 no.2 pp. 637-641
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; seedlings; hypocotyls; phytochrome; dose response; red light; far-red light; genetic variation; genotype; etiolation
Abstract:
... Using dichromatic radiation, we show that the actions of phytochromes A and B (phyA and phyB) in Arabidopsis thaliana are antagonistic in mediating red and far-red radiation effects on seedling de-etiolation and yet act in a complementary manner to regulate de-etiolation, irrespective of spectral composition. At low phytochrome photoequilibria inhibition of hypocotyl extension was strong, because ...

50. Antenna size dependency of photoinactivation of photosystem II in light-acclimated pea leaves

Author:
Park, Y.I.; Chow, W.S.; Anderson, J.M.
Source:
Plant physiology 1997 v.115 no.1 pp. 151-157
ISSN:
0032-0889
Subject:
Pisum sativum; leaves; photosystem II; photoinhibition; light intensity; acclimation; oxygen; gas exchange; chlorophyll; fluorescence; anaerobic conditions; light harvesting complex
Abstract:
... Utilization of absorbed light energy by photosystem (PS) II for O2 evolution depends on the light-harvesting antenna size, but the role of antenna size in the photoinactivation of PSII seems controversial. To address this controversy, pea (Pisum sativum L.) plants were grown in low (50 micromole m-2 s-1) or high (650 micromole m-2 s-1) light. The doubled functional antenna size of PSII in low ligh ...