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You searched for: Journal name Plant physiology Remove constraint Journal name: Plant physiology Publication year rev 8004-1996 Remove constraint Publication year rev: 8004-1996 Source 1996 v.111 no.4 Remove constraint Source: 1996 v.111 no.4
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1. Analysis of the expression of anthocyanin pathway genes in developing Vitis vinifera L. cv Shiraz grape berries and the implications for pathway regulation

Author:
Boss, P.K.; Davies, C.; Robinson, S.P.
Source:
Plant physiology 1996 v.111 no.4 pp. 1059-1066
ISSN:
0032-0889
Subject:
anthocyanins; flowering; biosynthesis; regulator genes; structural genes; biochemical pathways; gene expression; Vitis vinifera; fruiting
Abstract:
... Anthocyanin synthesis in Vitis vinifera L. cv Shiraz grape berries began 10 weeks postflowering and continued throughout berry ripening. Expression of seven genes of the anthocyanin biosynthetic pathway (phenylalanine ammonia lyase [PAL], chalcone synthase [CHS], chalcone isomerase [CHI], flavanone-3-hydroxylase [F3H], dihydroflavonol 4-reductase [DFR], leucoanthocyanidin dioxygenase [LDOX], and U ...

2. Arabidopsis thaliana sku mutant seedlings show exaggerated surface-dependent alteration in root growth vector

Author:
Rutherford, R.; Masson, P.H.
Source:
Plant physiology 1996 v.111 no.4 pp. 987-998
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; seedlings; mutants; ecotypes; roots; genes; tropisms; nutation; phenotype
Abstract:
... Roots of wild-type Arabidopsis thaliana seedlings in the Wassilewskija (WS) and Landsberg erecta (Ler) ecotypes often grow aslant on vertical agar surfaces. Slanted root growth always occurs to the right of the gravity vector when the root is viewed through the agar surface, and is not observed in the Columbia ecotype. Right-slanted root growth is surface-dependent and does not result directly fro ...
PubMed:
8756492
PubMed Central:
PMC160968

3. Central roles for potassium and sucrose in guard-cell osmoregulation

Author:
Talbott, L.D.; Zeiger, E.
Source:
Plant physiology 1996 v.111 no.4 pp. 1051-1057
ISSN:
0032-0889
Subject:
Vicia faba; leaves; osmoregulation; guard cells; potassium; sucrose; stomatal movement; diurnal variation; biochemical pathways; malic acid; chlorine; carbohydrate metabolism
Abstract:
... Osmoregulation in guard cells of intact, attached Vicia faba leaves grown under growth chamber and greenhouse conditions was studied over a daily light cycle of stomatal movements. Under both growth conditions guard cells had two distinct osmoregulatory phases. In the first (morning) phase, opening was correlated with K+ uptake and, to a lesser extent, sucrose accumulation. In the second (afternoo ...

4. Characterization of glutathione uptake in broad bean leaf protoplasts

Author:
Jamai, A.; Tommasini, R.; Martinoia, E.; Delrot, S.
Source:
Plant physiology 1996 v.111 no.4 pp. 1145-1152
ISSN:
0032-0889
Subject:
Vicia faba; leaves; protoplasts; organic sulfur compounds; glutathione; kinetics; uptake mechanisms; amino acids; translocation (plant physiology)
Abstract:
... Transport of reduced glutathione (GSH) and oxidized glutathione (GSSG) was studied with broad bean (Vicia faba L.) leaf tissues and protoplasts. Protoplasts and leaf discs took up GSSG at a rate about twice the uptake rate of GSH. Detailed studies with protoplasts indicated that GSH and GSSG uptake exhibited the samesensitivity to the external pH and to various chemical reagents. GSH uptake was in ...

5. Cloning of a cDNA encoding cytosolic acetoacetyl-coenzyme A thiolase from radish by functional expression in Saccharomyces cerevisiae

Author:
Vollack, K.U.; Bach, T.J.
Source:
Plant physiology 1996 v.111 no.4 pp. 1097-1107
ISSN:
0032-0889
Subject:
Raphanus sativus; acetyl-CoA acetyltransferase; cytosol; complementary DNA; Saccharomyces cerevisiae; mutants; genetic transformation; structural genes; gene expression; light; enzyme activity; genetic complementation; promoter regions
Abstract:
... A cDNA coding for radish (Raphanus sativus L.) acetoacetylcoenzyme A thiolase (AACT) was cloned by complementation of the erg10 mutation affecting AACT in yeast (Saccharomyces cerevisiae). The longest reading frame encodes a protein of 406 amino acids with a predicted relative molecular weight of 42,032, with significant similarities to eukaryotic and prokaryotic thiolases. There is no evidence fo ...
PubMed:
8756496
PubMed Central:
PMC160985

6. Cold-shock regulation of the Arabidopsis TCH genes and the effects of modulating intracellular calcium levels

Author:
Polisensky, D.H.; Braam, J.
Source:
Plant physiology 1996 v.111 no.4 pp. 1271-1279
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; transgenic plants; gene expression; cold stress; calmodulin; structural genes; calcium; cytosol
Abstract:
... The Arabidopsis TCH genes, which encode calmodulin-related proteins and a xyloglucan endotransglycosylase, are shown to be up-regulated in expression following cold shock. We investigated a possible role of fluctuations in intracellular calcium ion concentrations ([Ca2+]) in the cold-shock-inducedTCH gene expression. Transgenic plants harboring the apoaequorin gene were generated to monitor [Ca2+] ...
PubMed:
8756505
PubMed Central:
PMC161008

7. Competence for regeneration during tobacco internodal development. Involvement of plant age, cell elongation stage, and degree of polysomaty

Author:
Gilissen, L.J.W.; Staveren, M.J. van.; Hakkert, J.C.; Smulders, M.J.M.
Source:
Plant physiology 1996 v.111 no.4 pp. 1243-1250
ISSN:
0032-0889
Subject:
cortex; chemical composition; mixoploidy; diploidy; DNA; shoots; internodes; cells; Nicotiana tabacum; interphase; vegetative growth
Abstract:
... This study deals with internodal development in vegetative plants of Nicotiana tabacum cv Samsun NN and its reflection in changes of the cellular competence for regeneration. During elongation of the internodes, the cells of the epidermis, subepidermis, and cortex exclusively expanded and increased their DNA content cell type specifically, generally from 2C to 4C. Cells with the 8C DNA content wer ...

8. Complexity and genetic variability of heat-shock protein expression in isolated maize microspores

Author:
Magnard, J.L.; Vergne, P.; Dumas, C.
Source:
Plant physiology 1996 v.111 no.4 pp. 1085-1096
ISSN:
0032-0889
Subject:
Zea mays; pollen; germ cells; mitosis; genetic variation; heat stress; heat shock proteins; structural genes; gene expression; temperature; developmental stages; protein synthesis; genotype
Abstract:
... The expression of heat-shock proteins (HSPs) in isolated maize (Zea mays L.) microspores has been investigated using high-resolution two-dimensional electrophoresis coupled to immunodetection and fluorography of in vivo synthesized proteins. To this end, homogeneous and viable populations of microspores have been purified in sufficient amounts for molecular analysis from plants grown in controlled ...

9. Differential regulation of trichome formation on the adaxial and abaxial leaf surfaces by gibberellins and photoperiod in Arabidopsis thaliana (L.) Heynh

Author:
Chien, J.C.; Sussex, I.M.
Source:
Plant physiology 1996 v.111 no.4 pp. 1321-1328
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; paclobutrazol; flowering; ecotypes; spatial distribution; trichomes; gibberellic acid; mutants; photoperiod; leaves
Abstract:
... In wild-type (WT) Columbia and Landsberg erecta ecotypes of Arabidopsis thaliana (L.) Heynh., trichomes are present on the adaxial surfaces of all rosette leaves but are absent from the abaxial surfaces of the first-formed leaves. We have determined that both long-day (LD) photoperiod and gibberellin (GA) stimulate trichome formation. WT plants grown in LD conditions produce the first abaxial tric ...
PubMed:
8756507
PubMed Central:
PMC161017

10. Evolution and expression of C4 photosynthesis genes

Author:
Ku, M.S.B.; Kano-Murakami, Y.; Matsuoka, M.
Source:
Plant physiology 1996 v.111 no.4 pp. 949-957
ISSN:
0032-0889
Subject:
carbon dioxide; carbonate dehydratase; C3 photosynthesis; evolution; phosphoenolpyruvate carboxylase; Crassulacean acid metabolism; gene expression; carbon; genes; C4 photosynthesis; phosphotransferases (kinases); light
PubMed:
8756491
PubMed Central:
PMC160960

11. Hydroxycinnamoyltransferases involved in the accumulation of caffeic acid esters in gametophytes and sporophytes of Equisetum arvense

Author:
Hohlfeld, M.; Veit, M.; Strack, D.
Source:
Plant physiology 1996 v.111 no.4 pp. 1153-1159
ISSN:
0032-0889
Subject:
biosynthesis; caffeic acid; molecular weight; developmental stages; biochemical pathways; Equisetum arvense; pH; esters; kinetics; enzyme activity; acyltransferases
Abstract:
... Four hydroxycinnamoyltransferases from Equisetum arvense L. were studied that catalyze the formation of mono-O-caffeoyl-meso-tartrate, di-O-caffeoyl-meso-tartrate, 5-O-caffeoylshikimate (dactylifrate), and 5-O-caffeoylquinate (chlorogenate). The enzymes were classified as coenzyme A (CoA)-ester-dependent acyltransferases (EC 2.3.1), i.e. hydroxycinnamoyl-CoA:meso-tartrate hydroxycinnamoyltransfera ...

12. Isolation and characterization of glutamine synthetase from the maize diatom Skeletonema costatum

Author:
Robertson, D.L.; Alberte, R.S.
Source:
Plant physiology 1996 v.111 no.4 pp. 1169-1175
ISSN:
0032-0889
Subject:
Bacillariophyta; glutamate-ammonia ligase; molecular conformation; kinetics; enzyme activity; enzyme inhibitors; antibodies
Abstract:
... Two peaks of glutamine synthetase (GS) activity were resolved by anion-exchange chromatography from the marine diatom Skeletonema costatum Grev. The second peak of activity accounted for greater than 93% of total enzyme activity, and this isoform was purified over 200-fold. Results from denaturing gel electrophoresis and gel-filtration chromatography suggest that six 70-kD subunits constitute the ...

13. Membrane-delimited phosphorylation enables the activation of the outward-rectifying K channels in motor cell protoplasts of Samanea saman

Author:
Moran, N.
Source:
Plant physiology 1996 v.111 no.4 pp. 1281-1292
ISSN:
0032-0889
Subject:
turgor; Samanea saman; ion transport; adenosine triphosphate; magnesium; cytosol; potassium; electrophysiology; phosphorylation; plasma membrane; phosphotransferases (kinases); pulvinus; enzyme activity; proton pump
Abstract:
... Outward-rectifying K channels activated by membrane depolarization (Kout or KD channels) control K+ efflux from plant cells. To find out to what extent phosphorylation is required for the activity of these channels, the patch-clamp methodwas applied to protoplasts from the legume Samanea saman in both whole-cell and isolated-patch configurations. In the absence of either Mg2+ or ATP in the "cytoso ...

14. Molecular and structural changes in Chlamydomonas under limiting CO2. A possible mitochondrial role in adaptation

Author:
Geraghty, A.M.; Spalding, M.H.
Source:
Plant physiology 1996 v.111 no.4 pp. 1339-1347
ISSN:
0032-0889
Subject:
carbon dioxide; spatial distribution; starch; vacuoles; mitochondria; Chlamydomonas reinhardtii; chloroplasts; plant proteins
Abstract:
... When Chlamydomonas reinhardtii cells are transferred to limiting CO2, one response is the induction of a CO2-concentrating mechanism (CCM) with components that remain to be identified. Characterization of membrane-associated proteins induced by this transfer revealed that synthesis of the 21-kD protein (LIP-21) was regulated at the level of translatable message abundance and correlated well with t ...

15. Molecular characterization of the rehydration process in the resurrection plant Craterostigma plantagineum

Author:
Bernacchia, G.; Salamini, F.; Bartels, D.
Source:
Plant physiology 1996 v.111 no.4 pp. 1043-1050
ISSN:
0032-0889
Subject:
Scrophulariaceae; rehydration; water stress; gene expression; genes; protein synthesis
Abstract:
... The resurrection plant Craterostigma plantagineum is unique among higher plants because it is able to survive a desiccation treatment. For this reason it has been used as a model system for the analysis of the molecular mechanisms of desiccation tolerance. Many transcripts and proteins are expressed de novo duringdehydration. This paper describes the molecular events that occur during the rehydrat ...

16. Mutualistic fungal endophytes express a proteinase that is homologous to proteases suspected to be important in fungal pathogenicity

Author:
Reddy, P.V.; Lam, C.K.; Belanger, F.C.
Source:
Plant physiology 1996 v.111 no.4 pp. 1209-1218
ISSN:
0032-0889
Subject:
endophytes; Epichloe festucae var. lolii; serine proteinases; structural genes; entomopathogenic fungi; amino acid sequences; gene expression; plant pathogenic fungi; Acremonium; nucleotide sequences; mutualism; sequence homology; Poa secunda; virulence; nematophagous fungi
Abstract:
... Many cultivated and wild grass species are hosts to mutualistic fungal endophytes. These associations are ecologically and agronomically significant, yet little is known regarding the physiological aspects of the interaction. In the Poa ampla/Acremonium typhinum interaction, a fungal serine proteinase, At1, is surprisingly abundant and may constitute 1 to 2% of the total leaf-sheath protein. Seque ...
PubMed:
8756501
PubMed Central:
PMC160998

17. Novel osmotically induced antifungal chitinases and bacterial expression of an active recombinant isoform

Author:
Yun, D.J.; D'Urzo, M.P.; Abad, L.; Takeda, S.; Salzman, R.; Chen, Z.; Lee, H.; Hasegawa, P.M.; Bressan, R.A.
Source:
Plant physiology 1996 v.111 no.4 pp. 1219-1225
ISSN:
0032-0889
Subject:
structural genes; salinity; recombinant proteins; antifungal properties; defense mechanisms; water stress; gene expression; amino acid sequences; cultured cells; Nicotiana tabacum; plant pathogenic fungi; sequence homology; salt stress; chitinase
Abstract:
... NaCl (428 mM)-adapted tobacco (Nicotiana tabacum L. var Wisconsin 38) cells accumulate and secrete several antifungal chitinases. The predominant protein secreted to the culture medium was a 29-kD peptide that, based on internal amino acid sequence, was determined to be a class II acidic chitinase with similarity to PR-Q. The four predominant chitinases (T1, T2, T3, and T4) that accumulated intrac ...
PubMed:
8756502
PubMed Central:
PMC160999

18. Photosynthetic gene expression in meristems and during initial leaf development in a C4 dicotyledonous plant

Author:
Ramsperger, V.C.; Summers, R.G.; Berry, J.O.
Source:
Plant physiology 1996 v.111 no.4 pp. 999-1010
ISSN:
0032-0889
Subject:
ribulose-bisphosphate carboxylase; cell differentiation; messenger RNA; phosphoenolpyruvate carboxylase; Amaranthus hypochondriacus; gene expression; carbon; genes; C4 photosynthesis; phosphotransferases (kinases); enzyme activity; leaf primordia
Abstract:
... Immunolocalization and fluorescent in situ hybridization were used with confocal microscopy to examine patterns of photosynthetic gene expression during initial stages of leaf development in the C4 dicot Amaranthus hypochondriacus. mRNAs encoding the large and small subunit of ribulose-1,5-bisphosphate carboxylase were present in the apical dome and in all cells of the leaf primordia. In contrast, ...

19. Plasma membrane Na+ transport in a salt-tolerant charophyte. Isotopic fluxes, electrophysiology, and thermodynamics in plants adapted to saltwater and freshwater

Author:
Kiegle, E.A.; Bisson, M.A.
Source:
Plant physiology 1996 v.111 no.4 pp. 1191-1197
ISSN:
0032-0889
Subject:
salt tolerance; freshwater; plasma membrane; water quality; salinity; isotopes; Chara corallina; thermodynamics; cultured cells; sodium; pH; saline water; uptake mechanisms; electrophysiology
Abstract:
... In salt-tolerant Chara longifolia, enhanced Na+ efflux plays an important role in maintaining low cytoplasmic Na+. When it is cultured in fresh water (FW), C. longifolia has a higher Na+ efflux than the obligate FW Chara corallina, although pH dependence and inhibitor profiles are similar for both species (J. Whittington and M.A. Bisson [1994] J Exp Bot 45: 657-665). When it is cultured in saltwat ...

20. Predicting light acclimation in cyanobacteria from nonphotochemical quenching of photosystem II fluorescence, which reflects state transitions in these organisms

Author:
Campbell, D.; Oquist, G.
Source:
Plant physiology 1996 v.111 no.4 pp. 1293-1298
ISSN:
0032-0889
Subject:
fluorescence; light intensity; acclimation; electron transfer; photosystem II; Cyanobacteria; photoperiod; chlorophyll
Abstract:
... An important factor in photosynthetic ecophysiology is the light regime that a photobiont is acclimated to exploit. In a wide range of cyanobacteria and cyano-lichens, the easily measured fluorescence parameters, coefficient of nonphotochemical quenching of photosystem II variable fluorescence (qN) and nonphotochemical quenching, decline to a minimum near the acclimated growth light intensity. Thi ...