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beta-glucuronidase
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- Author:
- Bin Wu; Baocai Zhang; Yan Dai; Lei Zhang; Keke Shang-Guan; Yonggang Peng; Yihua Zhou; Zhen Zhu
- Source:
- Plant physiology 2012 v.159 no.4 pp. 1440-1452
- ISSN:
- 0032-0889
- Subject:
- Arabidopsis thaliana; Oryza sativa; beta-glucuronidase; bioinformatics; biosynthesis; cell walls; cellulose; chitinase; culms; disease resistance; genes; growth and development; membrane proteins; molecular cloning; mutants; mutation; plant growth; quantitative polymerase chain reaction; rice; sequence analysis; strength (mechanics)
- Abstract:
- ... Plant chitinases, a class of glycosyl hydrolases, participate in various aspects of normal plant growth and development, including cell wall metabolism and disease resistance. The rice (Oryza sativa) genome encodes 37 putative chitinases and chitinase-like proteins. However, none of them has been characterized at the genetic level. In this study, we report the isolation of a brittle culm mutant, b ...
- DOI:
- 10.1104/pp.112.195529
- PubMed:
- 22665444
- PubMed Central:
- PMC3425189
- https://doi.org/10.1104/pp.112.195529
- Author:
- Lu Qin; Jing Zhao; Jiang Tian; Liyu Chen; Zhaoan Sun; Yongxiang Guo; Xing Lu; Mian Gu; Guohua Xu; Hong Liao
- Source:
- Plant physiology 2012 v.159 no.4 pp. 1634-1643
- ISSN:
- 0032-0889
- Subject:
- Glycine max; agroecosystems; beta-glucuronidase; gene overexpression; genes; growth performance; heterologous gene expression; homeostasis; hydroponics; juveniles; nitrogen; nodulation; nutrition; phosphates; phosphorus; roots; soybeans; staining; sustainable agriculture; transgenic plants; vascular bundles; yeasts
- Abstract:
- ... Legume biological nitrogen (N) fixation is the most important N source in agroecosystems, but it is also a process requiring a considerable amount of phosphorus (P). Therefore, developing legume varieties with effective N ₂ fixation under P-limited conditions could have profound significance for improving agricultural sustainability. We show here that inoculation with effective rhizobial strains e ...
- DOI:
- 10.1104/pp.112.199786
- PubMed:
- 22740613
- PubMed Central:
- PMC3425202
- https://doi.org/10.1104/pp.112.199786
- Author:
- Venkatachalam Lakshmanan; Sherry L. Kitto; Jeffrey L. Caplan; Yi-Huang Hsueh; Daniel B. Kearns; Yu-Sung Wu; Harsh P. Bais
- Source:
- Plant physiology 2012 v.160 no.3 pp. 1642-1661
- ISSN:
- 0032-0889
- Subject:
- Arabidopsis thaliana; Bacillus subtilis; Pseudomonas syringae pv. tomato; antibiosis; beta-glucuronidase; biofilm; cell culture; culture filtrates; immune response; innate immunity; jasmonic acid; malates; malic acid; microbial communities; mutants; pathogenicity; pathogens; plant response; rhizosphere; rhizosphere bacteria; roots; rootstocks; salicylic acid; scions; secretion; signal transduction; systemic acquired resistance; tomatoes
- Abstract:
- ... This study demonstrated that foliar infection by Pseudomonas syringae pv tomato DC3000 induced malic acid (MA) transporter (ALUMINUM-ACTIVATED MALATE TRANSPORTER1 [ ALMT1 ]) expression leading to increased MA titers in the rhizosphere of Arabidopsis (Arabidopsis thaliana). MA secretion in the rhizosphere increased beneficial rhizobacteria Bacillus subtilis FB17 (hereafter FB17) titers causing an i ...
- DOI:
- 10.1104/pp.112.200386
- PubMed:
- 22972705
- PubMed Central:
- PMC3486800
- https://doi.org/10.1104/pp.112.200386
- Author:
- Zinnia Haydee González-Carranza; Ahmad Ali Shahid; Li Zhang; Yang Liu; Unchalee Ninsuwan; Jeremy Alan Roberts
- Source:
- Plant physiology 2012 v.160 no.3 pp. 1342-1356
- ISSN:
- 0032-0889
- Subject:
- Arabidopsis thaliana; RNA; abscission; beta-glucuronidase; cDNA libraries; crossing; dehiscence; ethylene; flowers; fluorescence; gene overexpression; genetically modified organisms; green fluorescent protein; indole acetic acid; microarray technology; mutants; phenotype; polygalacturonase; reporter genes; reverse transcriptase polymerase chain reaction; seedlings; tissues; transcription (genetics); transfer DNA
- Abstract:
- ... Abscission is the consequence of a specialized layer of cells undergoing a complex series of molecular and biochemical events. Analysis of the specific molecular changes associated with abscission is hampered by contamination from neighboring nonseparating tissues. Moreover, studies of abscission frequently involve the examination of events that take place in isolated segments of tissue exposed to ...
- DOI:
- 10.1104/pp.112.205955
- PubMed:
- 22992509
- PubMed Central:
- PMC3490599
- https://doi.org/10.1104/pp.112.205955
- Author:
- Sheppard, Anna E.; Ayliffe, Michael A.; Blatch, Laura; Day, Anil; Delaney, Sven K.; Khairul-Fahmy, Norfarhana; Li, Yuan; Madesis, Panagiotis; Pryor, Anthony J.; Timmis, Jeremy N.
- Source:
- Plant physiology 2008 v.148 no.1 pp. 328-336
- ISSN:
- 0032-0889
- Subject:
- Nicotiana tabacum; ancestry; beta-glucuronidase; chloroplast DNA; evolution; gametogenesis; germ cells; nuclear genome; organelles; ovules; parents; plant tissues; progeny; reciprocal crosses; reporter genes; screening; seedlings; somatic cells; tobacco; transposition (genetics)
- Abstract:
- ... In eukaryotes, many genes were transferred to the nucleus from prokaryotic ancestors of the cytoplasmic organelles during endosymbiotic evolution. In plants, the transfer of genetic material from the plastid (chloroplast) and mitochondrion to the nucleus is a continuing process. The cellular location of a kanamycin resistance gene tailored for nuclear expression (35SneoSTLS2) was monitored in the ...
- DOI:
- 10.1104/pp.108.119107
- PubMed:
- 18660434
- PubMed Central:
- PMC2528088
- https://doi.org/10.1104/pp.108.119107
- Author:
- Chen, Ing-Chien; Huang, I. Ching; Liu, Ming-Jung; Wang, Zhi-Gong; Chung, Shu-Shiang; Hsieh, Hsu-Liang
- Source:
- Plant physiology 2007 v.143 no.3 pp. 1189-1202
- ISSN:
- 0032-0889
- Subject:
- Arabidopsis thaliana; amino acids; beta-glucuronidase; cell growth; cytoplasm; enzyme activity; far-red light; flowering; gene expression; genes; glutathione transferase; messenger RNA; mutants; phenotype; phytochrome; signal transduction; vascular tissues; yeasts
- Abstract:
- ... Far-red (FR) insensitive 219 (FIN219) was previously shown to be involved in phytochrome A-mediated FR light signaling. To further understand its function and regulatory relation with other light-signaling components, a yeast two-hybrid approach was used to isolate FIN219-interacting partners. Here, we demonstrate that FIN219-interacting protein 1 (FIP1) interacts with FIN219 in vitro and in vivo ...
- DOI:
- 10.1104/pp.106.094185
- PubMed:
- 17220357
- PubMed Central:
- PMC1820923
- https://doi.org/10.1104/pp.106.094185
7. Agroinjection of Tomato Fruits. A Tool for Rapid Functional Analysis of Transgenes Directly in Fruit
- Author:
- Orzaez, Diego; Mirabel, Sophie; Wieland, Willemien H.; Granell, Antonio
- Source:
- Plant physiology 2006 v.140 no.1 pp. 3-11
- ISSN:
- 0032-0889
- Subject:
- tomatoes; genetic transformation; plant genetics; transgenes; transgenic plants; Agrobacterium radiobacter; reporter genes; genetic vectors; beta-glucuronidase; Solanum lycopersicoides; Solanum lycopersicum var. lycopersicum
- Abstract:
- ... Transient expression of foreign genes in plant tissues is a valuable tool for plant biotechnology. To shorten the time for gene functional analysis in fruits, we developed a transient methodology that could be applied to tomato (Solanum lycopersicum cv Micro Tom) fruits. It was found that injection of Agrobacterium cultures through the fruit stylar apex resulted in complete fruit infiltration. Thi ...
- DOI:
- 10.1104/pp.105.068221
- PubMed:
- 16403736
- PubMed Central:
- PMC1326026
- https://doi.org/10.1104/pp.105.068221
- Author:
- Zhiponova, Miroslava K.; Pettko-Szandtner, Aladar; Stelkovics, Eva; Neer, Zsuzsanna; Bottka, Sandor; Krenacs, Tibor; Dudits, Denes; Feher, Attila; Szilak, Laszlo
- Source:
- Plant physiology 2006 v.140 no.2 pp. 693-703
- ISSN:
- 0032-0889
- Subject:
- ethylene; signal transduction; alfalfa; plant damage; promoter regions; plant proteins; interphase; mitosis; complementary DNA; plant physiology; enzyme activation; plant genetics; forage crops; protein kinases; transgenic plants; Medicago sativa; reporter genes; beta-glucuronidase
- Abstract:
- ... Cyclin-dependent serine/threonine kinases (CDKs) have pivotal roles in regulating the eukaryotic cell cycle. Plants possess a unique class of CDKs (B-type CDKs) with preferential protein accumulation at G2/M-phases; however, their exact functions are still enigmatic. Here we describe the functional characterization of a 360-bp promoter region of the alfalfa (Medicago sativa) CDKB2;1 gene in transg ...
- DOI:
- 10.1104/pp.105.072173
- PubMed:
- 16407448
- PubMed Central:
- PMC1361335
- https://doi.org/10.1104/pp.105.072173
- Author:
- Wang, Bing-Bing; Brendel, Volker
- Source:
- Plant physiology 2006 v.140 no.2 pp. 624-636
- ISSN:
- 0032-0889
- Subject:
- Arabidopsis thaliana; plant genetics; alternative splicing; plant biochemistry; pleiotropy; ribonucleoproteins; conserved sequences; amino acid sequences; cell nucleus; phenotypic variation; promoter regions; beta-glucuronidase; plant proteins
- Abstract:
- ... U2AF (U2 small nuclear ribonucleoprotein auxiliary factor) is an essential splicing factor with critical roles in recognition of the 3'-splice site. In animals, the U2AF small subunit (U2AF³⁵) can bind to the 3'-AG intron border and promote U2 small nuclear RNP binding to the branch-point sequences of introns through interaction with the U2AF large subunit. Two copies of U2AF³⁵-encoding genes were ...
- DOI:
- 10.1104/pp.105.073858
- PubMed:
- 16407443
- PubMed Central:
- PMC1361329
- https://doi.org/10.1104/pp.105.073858
- Author:
- Shen, Yunping; Feng, Suhua; Ma, Ligeng; Lin, Rongcheng; Qu, Li-Jia; Chen, Zhangliang; Wang, Haiyang; Deng, Xing Wang
- Source:
- Plant physiology 2005 v.139 no.3 pp. 1234-1243
- ISSN:
- 0032-0889
- Subject:
- Arabidopsis thaliana; proteasome endopeptidase complex; beta-glucuronidase; transgenic plants; recombinant fusion proteins; nucleotide sequences; reporter genes; phytochrome; photoreceptors
- Abstract:
- ... Phytochrome A (phyA) is the primary photoreceptor mediating responses to far-red light. Among the phyA downstream signaling components, Far-red Elongated Hypocotyl 1 (FHY1) is a genetically defined positive regulator of photomorphogenesis in far-red light. Both physiological and genomic characterization of the fhy1 mutants indicated a close functional relationship of FHY1 with phyA. Here, we showe ...
- DOI:
- 10.1104/pp.105.067645
- PubMed:
- 16244150
- PubMed Central:
- PMC1283761
- https://doi.org/10.1104/pp.105.067645
- Author:
- Christmann, A.; Hoffmann, T.; Teplova, I.; Grill, E.; Muller, A.
- Source:
- Plant physiology 2005 v.137 no.1 pp. 209-219
- ISSN:
- 0032-0889
- Subject:
- Arabidopsis thaliana; root systems; transgenic plants; osmotic pressure; luciferase; tissue distribution; drought; water stress; plant vascular system; plant response; reporter genes; abscisic acid; stomata; promoter regions; beta-glucuronidase; shoots
- Abstract:
- ... A noninvasive, cell-autonomous reporter system was developed to monitor the generation and distribution of physiologically active pools of abscisic acid (ABA). ABA response (abi1-1) and biosynthesis (aba2-1) mutants of Arabidopsis (Arabidopsis thaliana) were used to validate the system in the presence and absence of water stress. In the absence of water stress, low levels of ABA-dependent reporter ...
- DOI:
- 10.1104/pp.104.053082
- PubMed:
- 15618419
- PubMed Central:
- PMC548852
- https://doi.org/10.1104/pp.104.053082
- Author:
- Subramanian, Senthil; Graham, Madge Y.; Yu, Oliver; Graham, Terrence L.
- Source:
- Plant physiology 2005 v.137 no.4 pp. 1345-1353
- ISSN:
- 0032-0889
- Subject:
- genetic transformation; RNA interference; Glycine max; messenger RNA; isoflavones; biosynthesis; Phytophthora sojae; transgenic plants; soybeans; cotyledons; Rhizobium rhizogenes; nucleotide sequences; reporter genes; oxygenases; beta-glucuronidase; plant proteins
- Abstract:
- ... Isoflavones are thought to play diverse roles in plant-microbe interactions and are also potentially important to human nutrition and medicine. Isoflavone synthase (IFS) is a key enzyme for the formation of the isoflavones. Here, we examined the consequences of RNAi silencing of genes for this enzyme in soybean (Glycine max). Soybean cotyledon tissues were transformed with Agrobacterium rhizogenes ...
- DOI:
- 10.1104/pp.104.057257
- PubMed:
- 15778457
- PubMed Central:
- PMC1088325
- https://doi.org/10.1104/pp.104.057257
- Author:
- Perera, Imara Y.; Hung, Chiu-Yueh; Brady, Shari; Muday, Gloria K.; Boss, Wendy F.
- Source:
- Plant physiology 2006 v.140 no.2 pp. 746-760
- ISSN:
- 0032-0889
- Subject:
- Arabidopsis thaliana; plant growth; inflorescences; transgenes; hypocotyls; inositol phosphates; phosphoric monoester hydrolases; transgenic plants; gravitropism; signal transduction; auxins; gravity; plant response; plant ecology; roots; beta-glucuronidase
- Abstract:
- ... Inositol 1,4,5-trisphosphate (InsP₃) has been implicated in the early signaling events of plants linking gravity sensing to the initiation of the gravitropic response. However, at present, the contribution of the phosphoinositide signaling pathway in plant gravitropism is not well understood. To delineate the role of InsP₃ in plant gravitropism, we generated Arabidopsis (Arabidopsis thaliana) plan ...
- DOI:
- 10.1104/pp.105.075119
- PubMed:
- 16384898
- PubMed Central:
- PMC1361340
- https://doi.org/10.1104/pp.105.075119
- Author:
- Zhao, Chengsong; Craig, Johanna C.; Petzold, H. Earl; Dickerman, Allan W.; Beers, Eric P.
- Source:
- Plant physiology 2005 v.138 no.2 pp. 803-818
- ISSN:
- 0032-0889
- Subject:
- signal transduction; roots; promoter regions; plant proteins; hypocotyls; biomarkers; transcriptome; gene expression regulation; Arabidopsis thaliana; apical meristems; messenger RNA; transcription (genetics); phloem; auxins; cambium; xylem; plant genetics; biosynthesis; physiological transport; reverse transcriptase polymerase chain reaction; cytokinins; reporter genes; beta-glucuronidase
- Abstract:
- ... The growth of secondary xylem and phloem depends on the division of cells in the vascular cambium and results in an increase in the diameter of the root and stem. Very little is known about the genetic mechanisms that control cambial activity and the differentiation of secondary xylem and phloem cell types. To begin to identify new genes required for vascular cell differentiation and function, we ...
- DOI:
- 10.1104/pp.105.060202
- PubMed:
- 15923329
- PubMed Central:
- PMC1150398
- https://doi.org/10.1104/pp.105.060202
- Author:
- Nishal, B.; Tantikanjana, T.; Sundaresan, V.
- Source:
- Plant physiology 2005 v.137 no.1 pp. 3-12
- ISSN:
- 0032-0889
- Subject:
- Arabidopsis thaliana; insertional mutagenesis; transposons; polymerase chain reaction; reporter genes; gene expression regulation; beta-glucuronidase
- Abstract:
- ... We describe a system of inducible insertional mutagenesis based on the Ac-Ds family of transposons for targeted tagging in Arabidopsis (Arabidopsis thaliana). In this system, the Ac and Ds elements are carried within the same T-DNA and a heat shock-inducible transposase fusion is utilized to control the levels of transposase gene expression, generating transpositions that can be subsequently stabi ...
- DOI:
- 10.1104/pp.104.050633
- PubMed:
- 15644463
- PubMed Central:
- PMC548834
- https://doi.org/10.1104/pp.104.050633
- Author:
- Barroco, R.M.; Poucke, K. van; Bergervoet, J.H.W.; Veylder, L. de; Groot, S.P.C.; Inze, D.; Engler, G.
- Source:
- Plant physiology 2005 v.137 no.1 pp. 127-140
- ISSN:
- 0032-0889
- Subject:
- Arabidopsis thaliana; seed germination; mitosis; messenger RNA; in situ hybridization; embryo (plant); gene expression regulation; reverse transcriptase polymerase chain reaction; cabbage; microtubules; reporter genes; Brassica oleracea; promoter regions; beta-glucuronidase; plant proteins
- Abstract:
- ... Cell cycle activity is required for plant growth and development, but its involvement in the early events that initiate seedling development remains to be clarified. We performed experiments aimed at understanding when cell cycle progression is activated during seed germination, and what its contribution is for proper seedling establishment. To this end, the spatial and temporal expression profile ...
- DOI:
- 10.1104/pp.104.049361
- PubMed:
- 15579664
- PubMed Central:
- PMC548844
- https://doi.org/10.1104/pp.104.049361
- Author:
- Ramirez-Chavez, E.; Lopez-Bucio, J.; Herrera-Estrella, L.; Molina-Torres, J.
- Source:
- Plant physiology 2004 v.134 no.3 pp. 1058-1068
- ISSN:
- 0032-0889
- Subject:
- Arabidopsis thaliana; plant growth; root systems; transgenes; cell growth; transgenic plants; amides; cell division; signal transduction; secondary metabolites; root hairs; rooting; auxins; chemical structure; Heliopsis; reporter genes; gene expression regulation; beta-glucuronidase
- Abstract:
- ... To date, several classes of hormones have been described that influence plant development, including auxins, cytokinins, ethylene, and, more recently, brassinosteroids. However, it is known that many fungal and bacterial species produce substances that alter plant growth that, if naturally present in plants, might represent novel classes of plant growth regulators. Alkamides are metabolites widely ...
- DOI:
- 10.1104/pp.103.034553
- PubMed:
- 14988477
- PubMed Central:
- PMC389930
- https://doi.org/10.1104/pp.103.034553
18. Analysis of the Female Gametophyte Transcriptome of Arabidopsis by Comparative Expression Profiling
- Author:
- Yu, Hee-Ju; Hogan, Pat; Sundaresan, Venkatesan
- Source:
- Plant physiology 2005 v.139 no.4 pp. 1853-1869
- ISSN:
- 0032-0889
- Subject:
- Arabidopsis thaliana; messenger RNA; plant genetics; gametophytes; embryo sac; transcription (genetics); transcriptome; gene fusion; statistical analysis; reporter genes; plant physiology; gene expression regulation; promoter regions; beta-glucuronidase
- Abstract:
- ... The extensive data on the transcription of the plant genome are derived primarily from the sporophytic generation. There currently is little information on genes that are expressed during female gametophyte development in angiosperms, and it is not known whether the female gametophyte transcriptome contains a major set of genes that are not expressed in the sporophyte or whether it is primarily a ...
- DOI:
- 10.1104/pp.105.067314
- PubMed:
- 16299181
- PubMed Central:
- PMC1310564
- https://doi.org/10.1104/pp.105.067314
- Author:
- Kobayashi, K.; Awai, K.; Takamiya, K.; Ohta, H.
- Source:
- Plant physiology 2004 v.134 no.2 pp. 640-648
- ISSN:
- 0032-0889
- Subject:
- cell membranes; galactosyltransferases; promoter regions; plant proteins; etioplasts; recombinant fusion proteins; tissue distribution; phosphates; nutrient solutions; gene expression regulation; Arabidopsis thaliana; transcription (genetics); pollen tubes; biosynthesis; transgenic plants; glycolipids; reporter genes; beta-glucuronidase
- Abstract:
- ... The galactolipids monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG) constitute the major glycolipids of the thylakoid membranes in chloroplasts. In Arabidopsis, the formation of MGDG is catalyzed by a family of three MGDG synthases, which are encoded by two types of genes, namely type A (atMGD1) and type B (atMGD2 and atMGD3). Although the roles of the type A enzyme have be ...
- DOI:
- 10.1104/pp.103.032656
- PubMed:
- 14730084
- PubMed Central:
- PMC344540
- https://doi.org/10.1104/pp.103.032656
- Author:
- Welchen, Elina; Gonzalez, Daniel H.
- Source:
- Plant physiology 2005 v.139 no.1 pp. 88-100
- ISSN:
- 0032-0889
- Subject:
- protein binding; plant extracts; benzyladenine; promoter regions; plant proteins; recombinant fusion proteins; meristems; tissue distribution; gene expression; mitochondria; gene expression regulation; Arabidopsis thaliana; anthers; sucrose; mutagenesis; cell nucleus; plant genetics; cytochrome c; cytokinins; cauliflower; reporter genes; DNA-binding proteins; Brassica oleracea var. botrytis; electron transport chain; beta-glucuronidase
- Abstract:
- ... The promoters of the Arabidopsis (Arabidopsis thaliana) cytochrome c genes, Cytc-1 and Cytc-2, were analyzed using plants transformed with fusions to the [beta]-glucuronidase coding sequence. Histochemical staining of plants indicated that the Cytc-1 promoter directs preferential expression in root and shoot meristems and in anthers. In turn, plants transformed with the Cytc-2 promoter fusions sho ...
- DOI:
- 10.1104/pp.105.065920
- PubMed:
- 16113211
- PubMed Central:
- PMC1203360
- https://doi.org/10.1104/pp.105.065920
- Author:
- Yang, Wei; Jefferson, Richard A.; Huttner, Eric; Moore, James M.; Gagliano, Wendy B.; Grossniklaus, Ueli
- Source:
- Plant physiology 2005 v.139 no.3 pp. 1421-1432
- ISSN:
- 0032-0889
- Subject:
- Arabidopsis thaliana; enhancer elements; plant genetics; gametophytes; plant reproduction; reporter genes; gene expression regulation; peroxidase; beta-glucuronidase
- Abstract:
- ... Despite a central role in angiosperm reproduction, few gametophyte-specific genes and promoters have been isolated, particularly for the inaccessible female gametophyte (embryo sac). Using the Ds-based enhancer-detector line ET253, we have cloned an egg apparatus-specific enhancer (EASE) from Arabidopsis (Arabidopsis thaliana). The genomic region flanking the Ds insertion site was further analyzed ...
- DOI:
- 10.1104/pp.105.068262
- PubMed:
- 16258010
- PubMed Central:
- PMC1283777
- https://doi.org/10.1104/pp.105.068262
- Author:
- Cheng, N.H.; Pittman, J.K.; Shigaki, T.; Lachmansingh, J.; LeClere, S.; Lahner, B.; Salt, D.E.; Hirschi, K.D.
- Source:
- Plant physiology 2005 v.138 no.4 pp. 2048-2060
- ISSN:
- 0032-0889
- Subject:
- Arabidopsis thaliana; plant growth; plant genetics; plant biochemistry; alleles; H-transporting ATPase; ions; recombinant fusion proteins; antiporters; gene expression; homeostasis; nucleotide sequences; calcium-binding proteins; beta-glucuronidase; plant proteins
- Abstract:
- ... Cation levels within the cytosol are coordinated by a network of transporters. Here, we examine the functional roles of calcium exchanger 1 (CAX1), a vacuolar H(+)/Ca(2+) transporter, and the closely related transporter CAX3. We demonstrate that like CAX1, CAX3 is also localized to the tonoplast. We show that CAX1 is predominately expressed in leaves, while CAX3 is highly expressed in roots. Previ ...
- Handle:
- 10113/7277
- DOI:
- 10.1104/pp.105.061218
- PubMed:
- 16055687
- PubMed Central:
- PMC1183394
- https://doi.org/10.1104/pp.105.061218
- Author:
- Groover, A.; Fontana, J.R.; Dupper, G.; Ma, C.; Martienssen, R.; Strauss, S.; Meilan, R.
- Source:
- Plant physiology 2004 v.134 no.4 pp. 1742-1751
- ISSN:
- 0032-0889
- Subject:
- enhancer elements; genetic transformation; plant development; hybrids; forest trees; insertional mutagenesis; Populus alba; transgenic plants; vascular tissues; developmental stages; transfer DNA; tissue distribution; amino acid sequences; reporter genes; Populus tremula; gene expression regulation; beta-glucuronidase
- Abstract:
- ... We report a gene discovery system for poplar trees based on gene and enhancer traps. Gene and enhancer trap vectors carrying the beta-glucuronidase (GUS) reporter gene were inserted into the poplar genome via Agrobacterium tumefaciens transformation, where they reveal the expression pattern of genes at or near the insertion sites. Because GUS expression phenotypes are dominant and are scored in pr ...
- Handle:
- 10113/14397
- DOI:
- 10.1104/pp.103.034330
- PubMed:
- 15051863
- PubMed Central:
- PMC419847
- https://doi.org/10.1104/pp.103.034330
- Author:
- Escobar, M.A.; Franklin, K.A.; Svensson, A.S.; Salter, M.G.; Whitelam, G.C.; Rasmusson, A.G.
- Source:
- Plant physiology 2004 v.136 no.1 pp. 2710-2721
- ISSN:
- 0032-0889
- Subject:
- Arabidopsis thaliana; promoter regions; beta-glucuronidase; recombinant fusion proteins; light intensity; adenosine triphosphate; cell respiration; transcription (genetics); gene induction; NADH or NADPH oxidoreductases; phytochrome; electron transport chain; photoreceptors
- Abstract:
- ... Controlled oxidation reactions catalyzed by the large, proton-pumping complexes of the respiratory chain generate an electrochemical gradient across the mitochondrial inner membrane that is harnessed for ATP production. However, several alternative respiratory pathways in plants allow the maintenance of substrate oxidation while minimizing the production of ATP. We have investigated the role of li ...
- DOI:
- 10.1104/pp.104.046698
- PubMed:
- 15333756
- PubMed Central:
- PMC523335
- https://doi.org/10.1104/pp.104.046698
- Author:
- Ezaki, B.; Suzuki, M.; Motoda, H.; Kawamura, M.; Nakashima, S.; Matsumoto, H.
- Source:
- Plant physiology 2004 v.134 no.4 pp. 1672-1682
- ISSN:
- 0032-0889
- Subject:
- Arabidopsis thaliana; sequence analysis; plant stress; beta-glucuronidase; transgenic plants; glutathione transferase; plant proteins; recombinant fusion proteins; signal transduction; aluminum; plant response; reporter genes; abiotic stress; gene induction; roots; promoter regions; leaves; shoots
- Abstract:
- ... The gene expression of two Al-induced Arabidopsis glutathione S-transferase genes, AtGST1 and AtGST11, was analyzed to investigate the mechanism underlying the response to Al stress. An approximately 1-kb DNA fragment of the 5'-upstream region of each gene was fused to a beta-glucuronidase (GUS) reporter gene (pAtGST1::GUS and pAtGST11::GUS) and introduced into Arabidopsis ecotype Landsberg erecta ...
- DOI:
- 10.1104/pp.103.037135
- PubMed:
- 15047894
- PubMed Central:
- PMC419841
- https://doi.org/10.1104/pp.103.037135
- Author:
- Park, H.C.; Kim, M.L.; Kang, Y.H.; Jeon, J.M.; Yoo, J.H.; Kim, M.C.; Park, C.Y.; Jeong, J.C.; Moon, B.C.; Lee, J.H.
- Source:
- Plant physiology 2004 v.135 no.4 pp. 2150-2161
- ISSN:
- 0032-0889
- Subject:
- plant pathogenic bacteria; transcription factors; Arabidopsis thaliana; Glycine max; beta-glucuronidase; transgenic plants; protein isoforms; soybeans; signal transduction; Pseudomonas syringae pv. tomato; amino acid sequences; sodium chloride; reporter genes; Pseudomonas syringae pv. glycinea; gene expression regulation; promoter regions; calmodulin; plant proteins
- Abstract:
- ... The Ca2+-binding protein calmodulin mediates cellular Ca2+ signals in response to a wide array of stimuli in higher eukaryotes. Plants express numerous CaM isoforms. Transcription of one soybean (Glycine max) CaM isoform, SCaM-4, is dramatically induced within 30 min of pathogen or NaCl stresses. To characterize the cis-acting element(s) of this gene, we isolated an approximately 2-kb promoter seq ...
- DOI:
- 10.1104/pp.104.041442
- PubMed:
- 15310827
- PubMed Central:
- PMC520786
- https://doi.org/10.1104/pp.104.041442
- Author:
- Harada, Hisashi; Nakatsuma, Daisuke; Ishida, Maki; Matsuda, Yusuke
- Source:
- Plant physiology 2005 v.139 no.2 pp. 1041-1050
- ISSN:
- 0032-0889
- Subject:
- carbon dioxide; carbonate dehydratase; messenger RNA; plant biochemistry; plant genetics; algae; acclimation; Phaeodactylum tricornutum; plant response; reporter genes; plant ecology; gene expression regulation; promoter regions; beta-glucuronidase; light; plant proteins
- Abstract:
- ... Cells of the marine diatom Phaeodactylum tricornutum Bohlin (UTEX 642) grown in 5% CO₂ were transferred to air-level CO₂ in the light or dark and allowed to acclimate to air. No accumulation of the transcript of the P. tricornutum [beta]-carbonic anhydrase 1 (ptca1) was detected in 5% CO₂-grown cells, but ptca1 mRNA accumulated and reached a peak after 6 h acclimation to air but decreased over the ...
- DOI:
- 10.1104/pp.105.065185
- PubMed:
- 16169965
- PubMed Central:
- PMC1256016
- https://doi.org/10.1104/pp.105.065185
- Author:
- Port, M.; Tripp, J.; Zielinski, D.; Weber, C.; Heerklotz, D.; Winkelhaus, S.; Bublak, D.; Scharf, K.D.
- Source:
- Plant physiology 2004 v.135 no.3 pp. 1457-1470
- ISSN:
- 0032-0889
- Subject:
- Solanum lycopersicum var. lycopersicum; plant proteins; transcription factors; transcription (genetics); heat shock proteins; protein-protein interactions; Nicotiana tabacum; tobacco; transgenic plants; protoplasts; protein aggregates; beta-glucuronidase; reporter genes; two hybrid system techniques; fluorescent antibody technique; amino acid sequences; nucleotide sequences
- Abstract:
- ... HsfA2 is a heat stress (hs)-induced Hsf in peruvian tomato (Lycopersicon peruvianum) and the cultivated form Lycopersicon esculentum. Due to the high activator potential and the continued accumulation during repeated cycles of heat stress and recovery, HsfA2 becomes a dominant Hsf in thermotolerant cells. The formation of heterooligomeric complexes with HsfA1 leads to nuclear retention and enhance ...
- DOI:
- 10.1104/pp.104.042820
- PubMed:
- 15247379
- PubMed Central:
- PMC519062
- https://doi.org/10.1104/pp.104.042820
- Author:
- Chen, M.H.; Huang, L.F.; Li, H.M.; Chen, Y.R.; Yu, S.M.
- Source:
- Plant physiology 2004 v.135 no.3 pp. 1367-1377
- ISSN:
- 0032-0889
- Subject:
- amyloplasts; rice; green fluorescent protein; grain crops; transgenes; cell walls; Oryza sativa; transgenic plants; microsomes; protein transport; signal peptide; Nicotiana tabacum; reporter genes; tobacco; gene expression regulation; alpha-amylase; beta-glucuronidase; chloroplasts; plant proteins
- Abstract:
- ... alpha-Amylases are important enzymes for starch degradation in plants. However, it has been a long-running debate as to whether alpha-amylases are localized in plastids where starch is stored. To study the subcellular localization of alpha-amylases in plant cells, a rice (Oryza sativa) alpha-amylase, alpha Amy3, with or without its own signal peptide (SP) was expressed in transgenic tobacco (Nicot ...
- DOI:
- 10.1104/pp.104.042184
- PubMed:
- 15235120
- PubMed Central:
- PMC519054
- https://doi.org/10.1104/pp.104.042184
- Author:
- Wu, Shuiqin; Schoenbeck, Mark A.; Greenhagen, Bryan T.; Takahashi, Shunji; Lee, Sungbeom; Coates, Robert M.; Chappell, Joseph
- Source:
- Plant physiology 2005 v.138 no.3 pp. 1322-1333
- ISSN:
- 0032-0889
- Subject:
- Arabidopsis thaliana; plant genetics; complementary DNA; Petunia hybrida; transgenic plants; RNA splicing; gene expression; Nicotiana tabacum; reporter genes; alkyl (aryl) transferases; introns; beta-glucuronidase; tobacco
- Abstract:
- ... A method for the recovery of full-length cDNAs from predicted terpene synthase genes containing introns is described. The approach utilizes Agrobacterium-mediated transient expression coupled with a reverse transcription-polydeoxyribonucleotide chain reaction assay to facilitate expression cloning of processed transcripts. Subsequent expression of intronless cDNAs in a suitable prokaryotic host pr ...
- DOI:
- 10.1104/pp.105.059386
- PubMed:
- 15965019
- PubMed Central:
- PMC1176406
- https://doi.org/10.1104/pp.105.059386
- Author:
- Meyer, S.; Lauterbach, C.; Niedermeier, M.; Barth, I.; Sjolund, R.D.; Sauer, N.
- Source:
- Plant physiology 2004 v.134 no.2 pp. 684-693
- ISSN:
- 0032-0889
- Subject:
- Arabidopsis thaliana; source-sink relationships; root tips; green fluorescent protein; plasmodesmata; plant damage; transporters; physiological transport; sieve elements; reporter genes; gene expression regulation; promoter regions; beta-glucuronidase; plant proteins
- Abstract:
- ... The Arabidopsis AtSUC3 gene encodes a sucrose (Suc) transporter that differs in size and intron number from all other Arabidopsis Suc transport proteins. Each plant species analyzed so far possesses one transporter of this special type, and several functions have been discussed for these proteins, including the catalysis of transmembrane Suc transport, and also Suc sensing and regulation of other ...
- DOI:
- 10.1104/pp.103.033399
- PubMed:
- 14739351
- PubMed Central:
- PMC344544
- https://doi.org/10.1104/pp.103.033399
- Author:
- Roberts, Gethin R.; Garoosi, G. Ali; Koroleva, Olga; Ito, Masaki; Laufs, Patrick; Leader, David J.; Caddick, Mark X.; Doonan, John H.; Tomsett, A. Brian
- Source:
- Plant physiology 2005 v.138 no.3 pp. 1259-1267
- ISSN:
- 0032-0889
- Subject:
- Nicotiana tabacum; tobacco; transgenic plants; cell suspension culture; Solanum lycopersicum var. lycopersicum; tomatoes; plant proteins; transcription factors; beta-glucuronidase; green fluorescent protein; reporter genes; gene expression regulation; tissue culture; plant genetics
- Abstract:
- ... The ALCR/alcA (alc) two-component, ethanol-inducible gene expression system provides stringent control of transgene expression in genetically modified plants. ALCR is an ethanol-activated transcription factor that can drive expression from the ALCR-responsive promoter (alcA). However, the alc system has been shown to have constitutive expression when used in plant callus or cell suspension culture ...
- DOI:
- 10.1104/pp.105.059659
- PubMed:
- 16010000
- PubMed Central:
- PMC1176399
- https://doi.org/10.1104/pp.105.059659
- Author:
- Scholz-Starke, J.; Buttner, M.; Sauer, N.
- Source:
- Plant physiology 2003 v.131 no.1 pp. 70-77
- ISSN:
- 0032-0889
- Subject:
- insertional mutagenesis; monosaccharides; developmental stages; pollen; promoter regions; nucleic acid hybridization; plant proteins; binding proteins; gene expression; membrane proteins; plasma membrane; Arabidopsis thaliana; messenger RNA; complementary DNA; transgenic plants; kinetics; Saccharomyces cerevisiae; fertilization (reproduction); reporter genes; pollen germination; chemical constituents of plants; beta-glucuronidase
- Abstract:
- ... This paper describes the molecular, kinetic, and physiological characterization of AtSTP6, a new member of the Arabidopsis H+/monosaccharide transporter family. The AtSTP6 gene (At3g05960) is interrupted by two introns and encodes a protein of 507 amino acids containing 12 putative transmembrane helices. Expression in yeast (Saccharomyces cerevisiae) shows that AtSTP6 is a high-affinity (K(m) = 20 ...
- PubMed:
- 12529516
- PubMed Central:
- PMC166788
- Author:
- Mattsson, J.; Ckurshumova, W.; Berleth, T.
- Source:
- Plant physiology 2003 v.131 no.3 pp. 1327-1339
- ISSN:
- 0032-0889
- Subject:
- Arabidopsis thaliana; transcription factors; plant development; transgenic plants; homeodomain proteins; physiological transport; signal transduction; gene expression; plant vascular system; mutants; indole acetic acid; leucine zipper; plant morphology; 2,4-D; promoter regions; beta-glucuronidase; leaves; plant proteins
- PubMed:
- 12644682
- PubMed Central:
- PMC166892
- Author:
- Nakamura, A.; Higuchi, K.; Goda, H.; Fujiwara, M.T.; Sawa, S.; Koshiba,T.; Shimada, Y.; Yoshida. S.
- Source:
- Plant physiology 2003 v.133 no.4 pp. 1843-1853
- ISSN:
- 0032-0889
- Subject:
- Arabidopsis thaliana; transgenic plants; plant proteins; messenger RNA; gene induction; transcription (genetics); brassinolide; indole acetic acid; signal transduction; beta-glucuronidase; reporter genes; nucleotide sequences
- Abstract:
- ... Despite numerous physiological studies addressing the interactions between brassinosteroids (BRs) and auxins, little is known about the underlying molecular mechanisms. We studied the expression of IAA5 and IAA19 in response to treatment with indole acetic acid (IAA) or brassinolide (BL), the most active BR. Exogenous IAA induced these genes quickly and transiently, whereas exogenous BL induced th ...
- DOI:
- 10.1104/pp.103.030031
- PubMed:
- 14605219
- PubMed Central:
- PMC300737
- https://doi.org/10.1104/pp.103.030031
- Author:
- Brugiere, N.; Jiao, S.; Hantke, S.; Zinselmeier, C.; Roessler, J.A.; Niu, X.O.; Jones, R.J.; Habben, J.E.
- Source:
- Plant physiology 2003 v.132 no.3 pp. 1228-1240
- ISSN:
- 0032-0889
- Subject:
- alleles; seedlings; roots; plant proteins; coleoptiles; dose response; open reading frames; tissue distribution; water stress; gene expression; heat stress; isopentenyladenine; Zea mays; plant development; messenger RNA; corn; vascular bundles; grain crops; transgenic plants; oxidoreductases; cytokinins; reporter genes; abscisic acid; beta-glucuronidase
- DOI:
- 10.1104/pp.102.017707
- PubMed:
- 12857805
- PubMed Central:
- PMC167063
- https://doi.org/10.1104/pp.102.017707
- Author:
- Weijers, D.; van Hamburg, J.P.; van Rijn, E.; Hooykaas, P.J.J.; Offringa, R.
- Source:
- Plant physiology 2003 v.133 no.4 pp. 1882-1892
- ISSN:
- 0032-0889
- Subject:
- Corynebacterium diphtheriae; bacterial proteins; transgenes; gene expression; Arabidopsis thaliana; seed development; endosperm; testa; embryo (plant); green fluorescent protein; beta-glucuronidase; reporter genes; transcriptional activation; cell communication
- Abstract:
- ... Fertilization of the female gametophyte in angiosperm plants initiates a process of coordinated development of embryo, endosperm, and seed coat that ensures the production of a viable seed. Mutant analysis has suggested that communication between the endosperm and the seed coat is an important determinant in this process. In addition, cell groups within the embryo, derived from the apical and from ...
- DOI:
- 10.1104/pp.103.030692
- PubMed:
- 14605218
- PubMed Central:
- PMC300741
- https://doi.org/10.1104/pp.103.030692
38. Extracellular ATP inhibits root gravitropism at concentrations that inhibit polar auxin transport
- Author:
- Tang, W.; Brady, S.R.; Sun, Y.; Muday, G.K.; Roux, S.J.
- Source:
- Plant physiology 2003 v.131 no.1 pp. 147-154
- ISSN:
- 0032-0889
- Subject:
- root tips; seedling growth; 2,4-D; dose response; naphthaleneacetic acid; plasma membrane; Arabidopsis thaliana; Zea mays; corn; adenosine triphosphate; translocation (plant physiology); culture media; indole acetic acid; food crops; gravitropism; transgenic plants; physiological transport; reporter genes; plant morphology; beta-glucuronidase
- Abstract:
- ... Raising the level of extracellular ATP to mM concentrations similar to those found inside cells can block gravitropism of Arabidopsis roots. When plants are grown in Murashige and Skoog medium supplied with 1 mM ATP, their roots grow horizontally instead of growing straight down. Medium with 2 mM ATP induces root curling, and 3 mM ATP stimulates lateral root growth. When plants are transferred to ...
- PubMed:
- 12529523
- PubMed Central:
- PMC166795
- Author:
- Harrar, Y.; Bellec, Y.; Bellini, C.; Faure, J.D.
- Source:
- Plant physiology 2003 v.132 no.3 pp. 1217-1227
- ISSN:
- 0032-0889
- Subject:
- Arabidopsis thaliana; protein kinases; homeodomain proteins; plant proteins; meristems; cell division; cell proliferation; auxins; cytokinins; reporter genes; nucleotide sequences; cell differentiation; gene expression regulation; beta-glucuronidase; cyclins
- DOI:
- 10.1104/pp.102.019026
- PubMed:
- 12857804
- PubMed Central:
- PMC167062
- https://doi.org/10.1104/pp.102.019026
- Author:
- Fourcroy, P.; Vansuyt, G.; Kushnir, S.; Inze, D.; Briat, J.F.
- Source:
- Plant physiology 2004 v.134 no.2 pp. 605-613
- ISSN:
- 0032-0889
- Subject:
- Arabidopsis thaliana; enhancer elements; ferritin; ascorbate peroxidase; transgenic plants; seedlings; iron; beta-glucuronidase; cytosol; cultured cells; reporter genes; gene induction; introns; roots; promoter regions; oxidative stress; plant proteins
- Abstract:
- ... Iron (Fe) is an essential element for living organisms. However, under aerobic conditions, its use is complicated because of its high insolubility and its potential toxicity through reactivity with reduced forms of oxygen. In plants, Fe overload can lead to intracellular concentrations beyond the storage and detoxification capacities of cells. Such a displacement toward a pro-oxidant state can act ...
- DOI:
- 10.1104/pp.103.029876
- PubMed:
- 14739345
- PubMed Central:
- PMC344537
- https://doi.org/10.1104/pp.103.029876
- Author:
- Bezanilla, M.; Pan, A.H.; Quatrano, R.S.
- Source:
- Plant physiology 2003 v.133 no.2 pp. 470-474
- ISSN:
- 0032-0889
- Subject:
- genetic transformation; green fluorescent protein; RNA interference; Physcomitrella patens; methodology; cytoplasm; cell nucleus; reporter genes; mosses and liverworts; beta-glucuronidase
- Abstract:
- ... The moss Physcomitrella patens performs efficient homologous recombination, which allows for the study of individual gene function by generating gene disruptions. Yet, if the gene of study is essential, gene disruptions cannot be isolated in the predominantly haploid P. patens. Additionally, disruption of a gene does not always generate observable phenotypes due to redundant functions from related ...
- DOI:
- 10.1104/pp.103.024901
- PubMed:
- 14555775
- PubMed Central:
- PMC523873
- https://doi.org/10.1104/pp.103.024901
- Author:
- Bhullar, S.; Chakravarthy, S.; Advani, S.; Datta, S.; Pental, D.; Burma, P.K.
- Source:
- Plant physiology 2003 v.132 no.2 pp. 988-998
- ISSN:
- 0032-0889
- Subject:
- genetic transformation; transgenes; experimental design; transgenic plants; seedlings; tissue distribution; gene expression; Nicotiana tabacum; Cauliflower mosaic virus; reporter genes; promoter regions; beta-glucuronidase
- DOI:
- 10.1104/pp.103.020602
- PubMed:
- 12805627
- PubMed Central:
- PMC167037
- https://doi.org/10.1104/pp.103.020602
- Author:
- Atanassova, R.; Leterrier, M.; Gaillard, C.; Agasse, A.; Sagot, E.; Coutos-Thevenot, P.; Delrot, S.
- Source:
- Plant physiology 2003 v.131 no.1 pp. 326-334
- ISSN:
- 0032-0889
- Subject:
- Nicotiana tabacum; tobacco; transgenic plants; cell lines; cell suspension culture; Vitis vinifera; grapes; fruit crops; plant proteins; binding proteins; messenger RNA; gene expression; glucose; sucrose; fructose; beta-glucuronidase; reporter genes; enzyme activity; cell growth; chemical constituents of plants; promoter regions; response elements; gene expression regulation; isomaltulose
- Abstract:
- ... Different lengths of the promoter of grape (Vitis vinifera) VvHT1 (Hexose Transporter 1) gene, which encodes a putative hexose transporter expressed during the ripening of grape, have been transcriptionally fused to the beta-glucuronidase reporter gene. In transgenic tobacco (Nicotiana tabacum) transformed with these constructs, VvHT1 promoters were clearly responsible for the sink organ preferent ...
- PubMed:
- 12529540
- PubMed Central:
- PMC166812
- Author:
- Mason, M.G.; Li, J.; Mathews, D.E.; Kieber, J.J.; Schaller, G.E.
- Source:
- Plant physiology 2004 v.135 no.2 pp. 927-937
- ISSN:
- 0032-0889
- Subject:
- Arabidopsis thaliana; cell nucleus; transcription factors; gene expression regulation; beta-glucuronidase; reporter genes; gene fusion; reverse transcriptase polymerase chain reaction; nucleotide sequences
- Abstract:
- ... Two-component signaling systems, involving His kinases, His-containing phosphotransfer proteins, and response regulators, have been implicated in plant responses to hormones and environmental factors. Genomic analysis of Arabidopsis supports the existence of 22 response regulators (ARRs) that can be divided into at least two distinct groups designated type-A and type-B. Phylogenetic analysis indic ...
- DOI:
- 10.1104/pp.103.038109
- PubMed:
- 15173562
- PubMed Central:
- PMC514127
- https://doi.org/10.1104/pp.103.038109
- Author:
- Baumberger, N.; Doesseger, B.; Guyot, R.; Diet, A.; Parsons, R.L.; Clark, M.A.; Simmons, M.P.; Bedinger, P.; Goff, S.A.; Ringli, C.
- Source:
- Plant physiology 2003 v.131 no.3 pp. 1313-1326
- ISSN:
- 0032-0889
- Subject:
- Arabidopsis thaliana; plant tissues; rice; grain crops; Oryza sativa; plant proteins; conserved sequences; recombinant fusion proteins; tissue distribution; amino acid sequences; gene expression; phylogeny; extensin; reporter genes; repetitive sequences; beta-glucuronidase; vegetative growth
- PubMed:
- 12644681
- PubMed Central:
- PMC166891
- Author:
- Lobstein, E.; Guyon, A.; Ferault, M.; Twell, D.; Pelletier, G.; Bonhomme, S.
- Source:
- Plant physiology 2004 v.135 no.3 pp. 1480-1490
- ISSN:
- 0032-0889
- Subject:
- Arabidopsis thaliana; transgenic plants; pollen tubes; plant growth; plant proteins; recombinant fusion proteins; complementary DNA; messenger RNA; conserved sequences; sequence alignment; beta-glucuronidase; reporter genes; Golgi apparatus; physiological transport; phenotype; Saccharomyces cerevisiae; amino acid sequences; nucleotide sequences
- Abstract:
- ... The screening of the Versailles collection of Arabidopsis T-DNA transformants allowed us to identify several male gametophytic mutants, including poky pollen tube (pok). The pok mutant, which could only be isolated as a hemizygous line, exhibits very short pollen tubes, explaining the male-specific transmission defect observed in this line. We show that the POK gene is duplicated in the Arabidopsi ...
- DOI:
- 10.1104/pp.103.037747
- PubMed:
- 15235115
- PubMed Central:
- PMC519064
- https://doi.org/10.1104/pp.103.037747
- Author:
- Avsian-Kretchmer, O.; Gueta-Dahan, Y.; Lev-Yadun, S.; Gollop, R.; Ben-Hayyim, G.
- Source:
- Plant physiology 2004 v.135 no.3 pp. 1685-1696
- ISSN:
- 0032-0889
- Subject:
- signal transduction; promoter regions; oxidative stress; plant proteins; recombinant fusion proteins; hydrogen peroxide; Nicotiana tabacum; gene expression regulation; enzyme inhibition; messenger RNA; complementary DNA; isozymes; salt stress; tobacco; catalase; transgenic plants; phospholipid-hydroperoxide glutathione peroxidase; Citrus sinensis; cultured cells; reporter genes; NADH or NADPH oxidoreductases; beta-glucuronidase
- Abstract:
- ... Several genes encoding putative glutathione peroxidase have been isolated from a variety of plants, all of which show the highest homology to the phospholipid hydroperoxide isoform. Several observations suggest that the proteins are involved in biotic and abiotic stress responses. Previous studies on the regulation of gpx1, the Citrus sinensis gene encoding phospholipid hydroperoxide isoform, led ...
- DOI:
- 10.1104/pp.104.041921
- PubMed:
- 15247370
- PubMed Central:
- PMC519082
- https://doi.org/10.1104/pp.104.041921
- Author:
- Satoh, R.; Nakashima, K.; Seki, M.; Shinozaki, K.; Yamaguchi-Shinozaki, K.
- Source:
- Plant physiology 2002 v.130 no.2 pp. 709-719
- ISSN:
- 0032-0889
- Subject:
- Arabidopsis thaliana; transgenic plants; luciferase; proline; repetitive sequences; gene expression; Nicotiana tabacum; reporter genes; chemical constituents of plants; osmolarity; plant morphology; promoter regions; beta-glucuronidase; tobacco; plant proteins
- Abstract:
- ... Proline (Pro) is one of the most widely distributed osmolytes in water-stressed plants. We previously isolated from Arabidopsis a gene encoding Pro dehydrogenase (ProDH), a mitochondrial enzyme involved in the first step of the conversion of Pro to glutamic acid. The ProDH gene in Arabidopsis is up-regulated by rehydration after dehydration but is down-regulated by dehydration. ProDH is also induc ...
- PubMed:
- 12376638
- PubMed Central:
- PMC166600
- Author:
- Perrin, R.M.; Jia, Z.; Wagner, T.A.; O'Neill, M.A.; Sarria, R.; York, W.S.; Raikhel, N.V.; Keegstra, K.
- Source:
- Plant physiology 2003 v.132 no.2 pp. 768-778
- ISSN:
- 0032-0889
- Subject:
- Arabidopsis thaliana; hexosyltransferases; plant tissues; messenger RNA; insertional mutagenesis; gene overexpression; xyloglucans; transgenic plants; glycosylation; transfer DNA; tissue distribution; acetylation; nucleotide sequences; reporter genes; enzyme activity; oligosaccharides; beta-glucuronidase; fucose; plant proteins
- DOI:
- 10.1104/pp.102.016642
- PubMed:
- 12805606
- PubMed Central:
- PMC167016
- https://doi.org/10.1104/pp.102.016642
- Author:
- Lu, S.X.; Hrabak, E.M.
- Source:
- Plant physiology 2002 v.128 no.3 pp. 1008-1021
- ISSN:
- 0032-0889
- Subject:
- Arabidopsis thaliana; endoplasmic reticulum; enzyme activity; detection; cytosol; cytoskeleton; cell membranes; cytochemistry; microsomes; recombinant proteins; beta-glucuronidase; biomarkers
- Abstract:
- ... Arabidopsis contains 34 genes that are predicted to encode calcium-dependent protein kinases (CDPKs). CDPK enzymatic activity previously has been detected in many locations in plant cells, including the cytosol, the cytoskeleton, and the membrane fraction. However, little is known about the subcellular locations of individual CDPKs or the mechanisms involved in targeting them to those locations. W ...
- DOI:
- 10.1104/pp.010770
- PubMed:
- 11891256
- PubMed Central:
- PMC152213
- https://doi.org/10.1104/pp.010770
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