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You searched for: Journal name Plant physiology Remove constraint Journal name: Plant physiology Subject beta-glucuronidase Remove constraint Subject: beta-glucuronidase
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1. The 5' flanking regions of vicilin and napin storage protein genes are down-regulated by desiccation in transgenic tobacco

Author:
Jiang, L.; Downing, W.L.; Baszczynski, C.L.; Kermode, A.R.
Source:
Plant physiology 1995 v.107 no.4 pp. 1439-1449
ISSN:
0032-0889
Subject:
gene transfer; vicilin; seeds; protein content; transgenic plants; developmental stages; seed development; gene expression; Nicotiana tabacum; reporter genes; enzyme activity; beta-glucuronidase
Abstract:
... Drying of seeds, when imposed prematurely, elicits a switch in metabolism; events unique to development, such as synthesis of storage protein, are terminated, whereas syntheses associated with germination andgrowth are initiated. To determine the role of desiccation in down-regulating the expression of genes for storage proteins, the desiccation responsiveness of the 5' and 3' regulatory regions o ...

2. ACTCAT, a novel cis-acting element for proline- and hypoosmolarity-responsive expression of the ProDH gene encoding proline dehydrogenase in Arabidopsis

Author:
Satoh, R.; Nakashima, K.; Seki, M.; Shinozaki, K.; Yamaguchi-Shinozaki, K.
Source:
Plant physiology 2002 v.130 no.2 pp. 709-719
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; transgenic plants; luciferase; proline; repetitive sequences; gene expression; Nicotiana tabacum; reporter genes; chemical constituents of plants; osmolarity; plant morphology; promoter regions; beta-glucuronidase; tobacco; plant proteins
Abstract:
... Proline (Pro) is one of the most widely distributed osmolytes in water-stressed plants. We previously isolated from Arabidopsis a gene encoding Pro dehydrogenase (ProDH), a mitochondrial enzyme involved in the first step of the conversion of Pro to glutamic acid. The ProDH gene in Arabidopsis is up-regulated by rehydration after dehydration but is down-regulated by dehydration. ProDH is also induc ...
PubMed:
12376638
PubMed Central:
PMC166600

3. Abscisic acid-dependent and -independent expression of the carrot late-embryogenesis-abundant-class gene Dc3 in transgenic tobacco seedlings

Author:
Siddiqui, N.U.; Chung, H.J.; Thomas, T.L.; Drew, M.C.
Source:
Plant physiology 1998 v.118 no.4 pp. 1181-1190
ISSN:
0032-0889
Subject:
genetic transformation; gene transfer; promoter regions; seedlings; Agrobacterium radiobacter; Daucus carota; transgenic plants; stress response; gene expression; histochemistry; Nicotiana tabacum; abscisic acid; enzyme activity; roots; beta-glucuronidase; shoots
Abstract:
... We studied the expression of three promoter 5' deletion constructs (-218, -599, and -1312) of the LEA (late embryogenesis abundant)-class gene Dc3 fused to beta-glucuronidase (GUS), where each construct value refers to the number of base pairs upstream of the transcription start site at which the deletion occurred. The Dc3 gene is noted for its induction by abscisic acid (ABA), but its response to ...

4. Activation of two osmotin-like protein genes by abiotic stimuli and fungal pathogen in transgenic potato plants

Author:
Zhu, B.; Chen, T.H.H.; Li, P.H.
Source:
Plant physiology 1995 v.108 no.3 pp. 929-937
ISSN:
0032-0889
Subject:
chimerism; salicylic acid; Agrobacterium; promoter regions; restriction mapping; gene expression; introns; defense mechanisms; Solanum commersonii; complementary DNA; Phytophthora infestans; transcription (genetics); sodium chloride; pathogenesis-related proteins; histochemistry; genetic transformation; gene transfer; transgenic plants; genomics; abscisic acid; beta-glucuronidase
Abstract:
... Osmotin-like proteins are encoded by at least six members of a multigene family in Solanum commersonii. A genomic clone (lambdapGEM2a-7) that contains two osmotin-like protein gene (OSML13 and OSML81) arranged in the same transcriptional orientation has been isolated. Restriction mapping and sequence analysis indicated that the two intronless genes correspond to the previously characterized pA13 a ...
PubMed:
7630973
PubMed Central:
PMC157442

5. Agrobacterium tumefaciens-mediated transformation of maize embryos using a standard binary vector system

Author:
Frame, B.R.; Shou, H.; Chikwamba, R.K.; Zhang, Z.; Xiang, C.; Fonger, T.M.; Pegg, S.E.K.; Li, B.; Nettleton, D.S.; Pei, D.
Source:
Plant physiology 2002 v.129 no.1 pp. 13-22
ISSN:
0032-0889
Subject:
genetic transformation; Zea mays; food crops; plant development; tillage; transgenic plants; embryo (plant); Agrobacterium radiobacter; transfer DNA; fertilization (reproduction); gene expression; zygote; genes; plant morphology; genetic vectors; beta-glucuronidase; genetic markers
Abstract:
... We have achieved routine transformation of maize (Zea mays) using an Agrobacterium tumefaciens standard binary (non-super binary) vector system. Immature zygotic embryos of the hybrid line Hi II were infected with A. tumefaciens strain EHA101 harboring a standard binary vector and cocultivated in the presence of 400 mg L1 L-cysteine. Inclusion of L-cysteine in cocultivation medium lead to an impro ...
PubMed:
12011333
PubMed Central:
PMC1540222

6. Agroinjection of Tomato Fruits. A Tool for Rapid Functional Analysis of Transgenes Directly in Fruit

Author:
Orzaez, Diego; Mirabel, Sophie; Wieland, Willemien H.; Granell, Antonio
Source:
Plant physiology 2006 v.140 no.1 pp. 3-11
ISSN:
0032-0889
Subject:
tomatoes; genetic transformation; plant genetics; transgenes; transgenic plants; Agrobacterium radiobacter; reporter genes; genetic vectors; beta-glucuronidase; Solanum lycopersicoides; Solanum lycopersicum var. lycopersicum
Abstract:
... Transient expression of foreign genes in plant tissues is a valuable tool for plant biotechnology. To shorten the time for gene functional analysis in fruits, we developed a transient methodology that could be applied to tomato (Solanum lycopersicum cv Micro Tom) fruits. It was found that injection of Agrobacterium cultures through the fruit stylar apex resulted in complete fruit infiltration. Thi ...
DOI:
10.1104/pp.105.068221
PubMed:
16403736
PubMed Central:
PMC1326026

7. Alkamides isolated from plants promote growth and alter root development in Arabidopsis

Author:
Ramirez-Chavez, E.; Lopez-Bucio, J.; Herrera-Estrella, L.; Molina-Torres, J.
Source:
Plant physiology 2004 v.134 no.3 pp. 1058-1068
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; plant growth; root systems; transgenes; cell growth; transgenic plants; amides; cell division; signal transduction; secondary metabolites; root hairs; rooting; auxins; chemical structure; Heliopsis; reporter genes; gene expression regulation; beta-glucuronidase
Abstract:
... To date, several classes of hormones have been described that influence plant development, including auxins, cytokinins, ethylene, and, more recently, brassinosteroids. However, it is known that many fungal and bacterial species produce substances that alter plant growth that, if naturally present in plants, might represent novel classes of plant growth regulators. Alkamides are metabolites widely ...
DOI:
10.1104/pp.103.034553
PubMed:
14988477
PubMed Central:
PMC389930

8. Analysis of gene promoters for two tomato polygalacturonases expressed in abscission zones and the stigma

Author:
Hong, S.B.; Sexton, R.; Tucker, M.L.
Source:
Plant physiology 2000 v.123 no.3 pp. 869-881
ISSN:
0032-0889
Subject:
ethylene; corolla; polygalacturonase; stigma; plant vascular system; promoter regions; calyx; gene expression; petioles; cortex; messenger RNA; abscission; enzyme inhibitors; enzyme activity; indole acetic acid; Solanum lycopersicum var. lycopersicum; transgenic plants; reporter genes; beta-glucuronidase
Abstract:
... The tomato (Lycopersicon esculentum cv Ailsa Craig) polygalacturonase genes TAPG1 (LYCes;Pga1;2) and TAPG4 (LYCes; Pga1;5) are abundantly expressed in both abscission zones and the pistils of mature flowers. To further investigate the spatial and temporal expression patterns for these genes, the TAPG gene promoters were ligated to beta-glucuronidase (GUS) reporter genes and transformed into tomato ...
PubMed:
10889236
PubMed Central:
PMC59050

9. Analysis of promoter activity for the gene encoding pyruvate orthophosphate dikinase in stably transformed C4 Flaveria species

Author:
Rosche, E.; Chitty, J.; Westhoff, P.; Taylor, W.C.
Source:
Plant physiology 1998 v.117 no.3 pp. 821-829
ISSN:
0032-0889
Subject:
orthophosphates; developmental stages; cytosol; phosphotransferases (kinases); roots; promoter regions; stems; gene expression; Flaveria trinervia; photosynthesis; mesophyll; pyruvic acid; genetic code; enzyme activity; vascular bundles; genetic transformation; gene transfer; transgenic plants; reporter genes; beta-glucuronidase; chloroplasts; Flaveria bidentis
Abstract:
... The C4 enzyme pyruvate orthophosphate dikinase is encoded by a single gene, Pdk, in the C4 plant Flaveria trinervia. This gene also encodes enzyme isoforms located in the chloroplast and in the cytosol that do not have a function in C4 photosynthesis. Our goal is to identify cis-acting DNA sequences that regulate the expression of the gene that is active in the C4 cycle. We fused 1.5 kb of a 5' fl ...

10. Analysis of the Female Gametophyte Transcriptome of Arabidopsis by Comparative Expression Profiling

Author:
Yu, Hee-Ju; Hogan, Pat; Sundaresan, Venkatesan
Source:
Plant physiology 2005 v.139 no.4 pp. 1853-1869
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; messenger RNA; plant genetics; gametophytes; embryo sac; transcription (genetics); transcriptome; gene fusion; statistical analysis; reporter genes; plant physiology; gene expression regulation; promoter regions; beta-glucuronidase
Abstract:
... The extensive data on the transcription of the plant genome are derived primarily from the sporophytic generation. There currently is little information on genes that are expressed during female gametophyte development in angiosperms, and it is not known whether the female gametophyte transcriptome contains a major set of genes that are not expressed in the sporophyte or whether it is primarily a ...
DOI:
10.1104/pp.105.067314
PubMed:
16299181
PubMed Central:
PMC1310564

11. Analysis of the expression of CLA1, a gene that encodes the 1-deoxyxylulose 5-phosphate synthase of the 2-C-methyl-D-erythritol-4-phosphate pathway in arabidopsis

Author:
Estevez, J.M.; Cantero, A.; Romero, C.; Kawaide, H.; Jimenez, L.F.; Kuzuyama, T.; Seto, H.; Kamiya, Y.; Leon, P.
Source:
Plant physiology 2000 v.124 no.1 pp. 95-103
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; messenger RNA; protein synthesis; phenotype; pleiotropy; isoprenoids; gene expression; ligases; mutants; reporter genes; genetic complementation; enzyme activity; promoter regions; beta-glucuronidase
Abstract:
... The discovery of the 2-C-methyl-D-erythritol-4-phosphate pathway for the biosynthesis of isoprenoids raises the important question of the nature and regulation of the enzymes involved in this pathway. CLA1, a gene previously isolated from Arabidopsis, encodes the first enzyme of the 2-C-methyl-D-erythritol-4-phosphate pathway, 1-deoxy-D-xylulose-5-phosphate synthase. We demonstrate this enzyme act ...
PubMed:
10982425
PubMed Central:
PMC59125

12. Analysis of xyloglucan fucosylation in Arabidopsis

Author:
Perrin, R.M.; Jia, Z.; Wagner, T.A.; O'Neill, M.A.; Sarria, R.; York, W.S.; Raikhel, N.V.; Keegstra, K.
Source:
Plant physiology 2003 v.132 no.2 pp. 768-778
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; hexosyltransferases; plant tissues; messenger RNA; insertional mutagenesis; gene overexpression; xyloglucans; transgenic plants; glycosylation; transfer DNA; tissue distribution; acetylation; nucleotide sequences; reporter genes; enzyme activity; oligosaccharides; beta-glucuronidase; fucose; plant proteins
DOI:
10.1104/pp.102.016642
PubMed:
12805606
PubMed Central:
PMC167016

13. Arabidopsis FHY1 Protein Stability Is Regulated by Light via Phytochrome A and 26S Proteasome

Author:
Shen, Yunping; Feng, Suhua; Ma, Ligeng; Lin, Rongcheng; Qu, Li-Jia; Chen, Zhangliang; Wang, Haiyang; Deng, Xing Wang
Source:
Plant physiology 2005 v.139 no.3 pp. 1234-1243
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; proteasome endopeptidase complex; beta-glucuronidase; transgenic plants; recombinant fusion proteins; nucleotide sequences; reporter genes; phytochrome; photoreceptors
Abstract:
... Phytochrome A (phyA) is the primary photoreceptor mediating responses to far-red light. Among the phyA downstream signaling components, Far-red Elongated Hypocotyl 1 (FHY1) is a genetically defined positive regulator of photomorphogenesis in far-red light. Both physiological and genomic characterization of the fhy1 mutants indicated a close functional relationship of FHY1 with phyA. Here, we showe ...
DOI:
10.1104/pp.105.067645
PubMed:
16244150
PubMed Central:
PMC1283761

14. Arabidopsis RTM1 and RTM2 genes function in phloem to restrict long-distance movement of tobacco etch virus

Author:
Chisholm, S.T.; Parra, M.A.; Anderberg, R.J.; Carrington, J.C.
Source:
Plant physiology 2001 v.127 no.4 pp. 1667-1675
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; regulatory sequences; plant proteins; sieve elements; disease resistance; reporter genes; induced resistance; Tobacco etch virus; beta-glucuronidase; defense mechanisms
Abstract:
... Restriction of long-distance movement of tobacco etch virus (TEV) in Arabidopsis ecotype Col-0 plants requires the function of at least three genes: RTM1 (restricted TEV movement 1), RTM2, and RTM3. The mechanism of TEV movement restriction remains poorly understood, although it does not involve a hypersensitive response or systemic acquired resistance. A functional characterization of RTM1 and RT ...
DOI:
10.1104/pp.010479
PubMed:
11743111
PubMed Central:
PMC133571

15. Arabidopsis alcohol dehydrogenase expression in both shoots and roots is conditioned by root growth environment

Author:
Chung, H.J.; Ferl, R.J.
Source:
Plant physiology 1999 v.121 no.2 pp. 429-436
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; alcohol dehydrogenase; promoter regions; transgenic plants; soil; gene expression; reporter genes; hypoxia; roots; beta-glucuronidase; shoots
Abstract:
... It is widely accepted that the Arabidopsis Adh (alcohol dehydrogenase) gene is constitutively expressed at low levels in the roots of young plants grown on agar media, and that the expression level is greatly induced by anoxic or hypoxic stresses. We questioned whether the agar medium itself created an anaerobic environment for the roots upon their growing into the gel. beta-Glucuronidase (GUS) ex ...
PubMed:
10517834
PubMed Central:
PMC59405

16. An Arabidopsis calcium-dependent protein kinase is associated with the endoplasmic reticulum

Author:
Lu, S.X.; Hrabak, E.M.
Source:
Plant physiology 2002 v.128 no.3 pp. 1008-1021
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; endoplasmic reticulum; enzyme activity; detection; cytosol; cytoskeleton; cell membranes; cytochemistry; microsomes; recombinant proteins; beta-glucuronidase; biomarkers
Abstract:
... Arabidopsis contains 34 genes that are predicted to encode calcium-dependent protein kinases (CDPKs). CDPK enzymatic activity previously has been detected in many locations in plant cells, including the cytosol, the cytoskeleton, and the membrane fraction. However, little is known about the subcellular locations of individual CDPKs or the mechanisms involved in targeting them to those locations. W ...
DOI:
10.1104/pp.010770
PubMed:
11891256
PubMed Central:
PMC152213

17. Arabidopsis type B monogalactosyldiacylglycerol synthase genes are expressed during pollen tube growth and induced by phosphate starvation

Author:
Kobayashi, K.; Awai, K.; Takamiya, K.; Ohta, H.
Source:
Plant physiology 2004 v.134 no.2 pp. 640-648
ISSN:
0032-0889
Subject:
cell membranes; galactosyltransferases; promoter regions; plant proteins; etioplasts; recombinant fusion proteins; tissue distribution; phosphates; nutrient solutions; gene expression regulation; Arabidopsis thaliana; transcription (genetics); pollen tubes; biosynthesis; transgenic plants; glycolipids; reporter genes; beta-glucuronidase
Abstract:
... The galactolipids monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG) constitute the major glycolipids of the thylakoid membranes in chloroplasts. In Arabidopsis, the formation of MGDG is catalyzed by a family of three MGDG synthases, which are encoded by two types of genes, namely type A (atMGD1) and type B (atMGD2 and atMGD3). Although the roles of the type A enzyme have be ...
DOI:
10.1104/pp.103.032656
PubMed:
14730084
PubMed Central:
PMC344540

18. The Arabidopsis xylem peptidase XCP1 is a tracheary element vacuolar protein that may be a papain ortholog

Author:
Funk, V.; Kositsup, B.; Zhao, C.; Beers, E.P.
Source:
Plant physiology 2002 v.128 no.1 pp. 84-94
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; papain; tracheids; vacuoles; reporter genes; chemical constituents of plants; chlorophyll; promoter regions; beta-glucuronidase; leaves
Abstract:
... XCP1 is a xylem-specific papain-like cysteine peptidase in Arabidopsis. To determine whether XCP1 could be involved in tracheary element autolysis, promoter activity and localization of XCP1 were investigated using XCP1 promoter-beta-glucuronidase fusions and immunofluorescence confocal microscopy. A tracheary element expression pattern was detected for XCP1. Results from confocal microscopy and b ...
DOI:
10.1104/pp.010514
PubMed:
11788755
PubMed Central:
PMC148946

19. AtSTP6, a new pollen-specific H+-monosaccharide symporter from Arabidopsis

Author:
Scholz-Starke, J.; Buttner, M.; Sauer, N.
Source:
Plant physiology 2003 v.131 no.1 pp. 70-77
ISSN:
0032-0889
Subject:
insertional mutagenesis; monosaccharides; developmental stages; pollen; promoter regions; nucleic acid hybridization; plant proteins; binding proteins; gene expression; membrane proteins; plasma membrane; Arabidopsis thaliana; messenger RNA; complementary DNA; transgenic plants; kinetics; Saccharomyces cerevisiae; fertilization (reproduction); reporter genes; pollen germination; chemical constituents of plants; beta-glucuronidase
Abstract:
... This paper describes the molecular, kinetic, and physiological characterization of AtSTP6, a new member of the Arabidopsis H+/monosaccharide transporter family. The AtSTP6 gene (At3g05960) is interrupted by two introns and encodes a protein of 507 amino acids containing 12 putative transmembrane helices. Expression in yeast (Saccharomyces cerevisiae) shows that AtSTP6 is a high-affinity (K(m) = 20 ...
PubMed:
12529516
PubMed Central:
PMC166788

20. Auxin and ethylene response interactions during Arabidopsis root hair development dissected by auxin influx modulators

Author:
Rahman, A.; Hosokawa, S.; Oono, Y.; Amakawa, T.; Goto, N.; Tsurumi, S.
Source:
Plant physiology 2002 v.130 no.4 pp. 1908-1917
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; ethylene; plant development; glycolic acid; phenotype; transgenic plants; seedlings; root hairs; saponins; mutants; reporter genes; naphthaleneacetic acid; naphthalene; plant morphology; beta-glucuronidase; indole acetic acid
Abstract:
... The plant hormones auxin and ethylene have been shown to play important roles during root hair development. However, cross talk between auxin and ethylene makes it difficult to understand the independent role of either hormone. To dissect their respective roles, we examined the effects of two compounds, chromosaponin I (CSI) and 1-naphthoxyacetic acid (1-NOA), on the root hair developmental proces ...
PubMed:
12481073
PubMed Central:
PMC166701

21. Auxin signaling in Arabidopsis leaf vascular development

Author:
Mattsson, J.; Ckurshumova, W.; Berleth, T.
Source:
Plant physiology 2003 v.131 no.3 pp. 1327-1339
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; transcription factors; plant development; transgenic plants; homeodomain proteins; physiological transport; signal transduction; gene expression; plant vascular system; mutants; indole acetic acid; leucine zipper; plant morphology; 2,4-D; promoter regions; beta-glucuronidase; leaves; plant proteins
PubMed:
12644682
PubMed Central:
PMC166892

22. BWMK1, a rice mitogen-activated protein kinase, locates in the nucleus and mediates pathogenesis-related gene expression by activation of a transcription factor

Author:
Cheong, Y.H.; Moon, B.C.; Kim, J.K.; Kim, C.Y.; Kim, M.C.; Kim, I.H.; Park, C.Y.; Kim, J.C.; Park, B.O.; Koo, S.C.
Source:
Plant physiology 2003 v.132 no.4 pp. 1961-1972
ISSN:
0032-0889
Subject:
rice; Oryza sativa; signal transduction; phosphorylation; mitogen-activated protein kinase; plant proteins; recombinant fusion proteins; fungal diseases of plants; Nicotiana tabacum; disease resistance; Arabidopsis thaliana; transcription factors; gene overexpression; bacterial diseases of plants; amino acid sequences; resistance mechanisms; cell nucleus; Phytophthora nicotianae var. parasitica; tobacco; enzyme activation; grain crops; transgenic plants; DNA-binding proteins; reporter genes; nucleotide sequences; beta-glucuronidase; Pseudomonas syringae pv. tabaci
Abstract:
... Mitogen-activated protein kinase (MAPK) cascades are known to transduce plant defense signals, but the downstream components of the MAPK have as yet not been elucidated. Here, we report an MAPK from rice (Oryza sativa), BWMK1, and a transcription factor, OsEREBP1, phosphorylated by the kinase. The MAPK carries a TDY phosphorylation motif instead of the more common TEY motif in its kinase domain an ...
DOI:
10.1104/pp.103.023176
PubMed:
12913152
PubMed Central:
PMC181281

23. Brassinolide induces IAA5, IAA19, and DR5, a synthetic auxin response element in arabidopsis implying a cross talk point of brassinosteroid and auxin signaling

Author:
Nakamura, A.; Higuchi, K.; Goda, H.; Fujiwara, M.T.; Sawa, S.; Koshiba,T.; Shimada, Y.; Yoshida. S.
Source:
Plant physiology 2003 v.133 no.4 pp. 1843-1853
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; transgenic plants; plant proteins; messenger RNA; gene induction; transcription (genetics); brassinolide; indole acetic acid; signal transduction; beta-glucuronidase; reporter genes; nucleotide sequences
Abstract:
... Despite numerous physiological studies addressing the interactions between brassinosteroids (BRs) and auxins, little is known about the underlying molecular mechanisms. We studied the expression of IAA5 and IAA19 in response to treatment with indole acetic acid (IAA) or brassinolide (BL), the most active BR. Exogenous IAA induced these genes quickly and transiently, whereas exogenous BL induced th ...
DOI:
10.1104/pp.103.030031
PubMed:
14605219
PubMed Central:
PMC300737

24. Brittle Culm15 Encodes a Membrane-Associated Chitinase-Like Protein Required for Cellulose Biosynthesis in Rice

Author:
Bin Wu; Baocai Zhang; Yan Dai; Lei Zhang; Keke Shang-Guan; Yonggang Peng; Yihua Zhou; Zhen Zhu
Source:
Plant physiology 2012 v.159 no.4 pp. 1440-1452
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; Oryza sativa; beta-glucuronidase; bioinformatics; biosynthesis; cell walls; cellulose; chitinase; culms; disease resistance; genes; growth and development; membrane proteins; molecular cloning; mutants; mutation; plant growth; quantitative polymerase chain reaction; rice; sequence analysis; strength (mechanics)
Abstract:
... Plant chitinases, a class of glycosyl hydrolases, participate in various aspects of normal plant growth and development, including cell wall metabolism and disease resistance. The rice (Oryza sativa) genome encodes 37 putative chitinases and chitinase-like proteins. However, none of them has been characterized at the genetic level. In this study, we report the isolation of a brittle culm mutant, b ...
DOI:
10.1104/pp.112.195529
PubMed:
22665444
PubMed Central:
PMC3425189

25. Cambial-region-specific expression of the Agrobacterium iaa genes in transgenic aspen visualized by a linked uidA reporter gene

Author:
Tuominen, H.; Puech, L.; Regan, S.; Fink, S.; Olsson, O.; Sundberg, B.
Source:
Plant physiology 2000 v.123 no.2 pp. 531-541
ISSN:
0032-0889
Subject:
Populus tremula; Populus tremuloides; cambium; transgenic plants; genetic transformation; beta-glucuronidase; reporter genes; enzyme activity; plant development; xylem; indole acetic acid; histochemistry
PubMed:
10859183
PubMed Central:
PMC59021

26. Carbon and amino acids reciprocally modulate the expression of glutamine synthetase in Arabidopsis

Author:
Oliveira, I.C.; Coruzzi, G.M.
Source:
Plant physiology 1999 v.121 no.1 pp. 301-309
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; protein synthesis; messenger RNA; phytochrome; nitrogen; sucrose; enzyme activity; glutamate-ammonia ligase; transcription (genetics); gene expression; carbon; amino acids; chromosome mapping; reporter genes; chemical constituents of plants; multigene family; promoter regions; beta-glucuronidase; light
Abstract:
... In bacteria and yeast, glutamine synthetase (GS) expression is tightly regulated by the metabolic status of the cell, both at the transcriptional and posttranscriptional levels. We discuss the relative contributions of light and metabolic cues on the regulation of members of the GS gene family (chloroplastic GS2 and cytosolic GS1) in Arabidopsis. These studies reveal that the dramatic induction of ...
PubMed:
10482686
PubMed Central:
PMC59385

27. Cell-specific and conditional expression of caffeoyl-coenzyme A-3-O-methyltransferase in poplar

Author:
Chen, C.; Meyermans, H.; Burggraeve, B.; Rycke, R.M. de.; Inoue, K.; Vleesschauwer, V. de.; Steenackers, M.; Montagu, M.C. van.; Engler, G.J.; Boerjan, W.A.
Source:
Plant physiology 2000 v.123 no.3 pp. 853-867
ISSN:
0032-0889
Subject:
promoter regions; protein synthesis; stems; transgenic plants; Populus trichocarpa; gene expression; phloem; multigene family; reporter genes; xylem; transferases; beta-glucuronidase
Abstract:
... Caffeoyl coenzyme A-3-O-methyltransferase (CCoAOMT) plays an important role in lignin biosynthesis and is encoded by two genes in poplar (Populus trichocarpa). Here, we describe the expression pattern conferred by the two CCoAOMT promoters when fused to the gus-coding sequence in transgenic poplar (Populus tremula X Populus alba). Both genes were expressed similarly in xylem and differentially in ...
PubMed:
10889235
PubMed Central:
PMC59049

28. Cell-specific expression of the promoters of two nonlegume hemoglobin genes in a transgenic legume, Lotus corniculatus

Author:
Andersson, C.R.; Llewellyn, D.J.; Peacock, W.J.; Dennis, E.S.
Source:
Plant physiology 1997 v.113 no.1 pp. 45-57
ISSN:
0032-0889
Subject:
developmental stages; promoter regions; Parasponia; nitrogen fixation; gene expression; Casuarina glauca; Lotus corniculatus; leghemoglobin; root nodules; amino acid sequences; transcription (genetics); Rhizobium; enzyme activity; histochemistry; Trema; gene transfer; symbiosis; transgenic plants; hemoglobin; reporter genes; beta-glucuronidase
Abstract:
... The promoters of the hemoglobin genes from the nitrogen-fixing tree Parasponia andersonii and the related nonnitrogen-fixing Trema tomentosa both confer beta-glucuronidase reporter gene expression to the central zone of the nodules of a transgenic legume, Lotus corniculatus. beta-Glucuronidase expression was high in the uninfected interstitial cells and parenchyma of the surrounding boundary layer ...
PubMed:
9008386
PubMed Central:
PMC158114

29. Changes in gene expression in Arabidopsis shoots during phosphate starvation and the potential for developing smart plants

Author:
Hammond, J.P.; Bennett, M.J.; Bowen, H.C.; Broadley, M.R.; Eastwood, D.C.; May, S.T.; Rahn, C.; Swarup, R.; Woolaway, K.E.; White, P.J.
Source:
Plant physiology 2003 v.132 no.2 pp. 578-596
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; genetic transformation; plant breeding; TATA box; transgenic plants; enzyme activity; nutrient deficiencies; hydroponics; phosphates; reporter genes; nutrient solutions; phenotypic variation; phosphorus; gene expression regulation; beta-glucuronidase; shoots
DOI:
10.1104/pp.103.020941
PubMed:
12805589
PubMed Central:
PMC166999

30. Characterization and expression of four proline-rich cell wall protein genes in Arabidopsis encoding two distinct subsets of multiple domain proteins

Author:
Fowler, T.J.; Bernhardt, C.; Tierney, M.L.
Source:
Plant physiology 1999 v.121 no.4 pp. 1081-1091
ISSN:
0032-0889
Subject:
nucleic acid hybridization; roots; promoter regions; plant proteins; stems; cell walls; gene expression; cell wall components; introns; leaves; Arabidopsis thaliana; messenger RNA; complementary DNA; amino acid sequences; chromosome mapping; pods; flowers; reporter genes; beta-glucuronidase
Abstract:
... We have characterized the molecular organization and expression of four proline-rich protein genes from Arabidopsis (AtPRPs). These genes predict two classes of cell wall proteins based on DNA sequence identity, repetitive motifs, and domain organization. AtPRP1 and AtPRP3 encode proteins containing an N-terminal PRP-like domain followed by a C-terminal domain that is biased toward P, T, Y, and K. ...
PubMed:
10594096
PubMed Central:
PMC59476

31. Characterization of Arabidopsis AtAMT2, a high-affinity ammonium transporter of the plasma membrane

Author:
Sohlenkamp, C.; Wood, C.C.; Roeb, G.W.; Udvardi, M.K.
Source:
Plant physiology 2002 v.130 no.4 pp. 1788-1796
ISSN:
0032-0889
Subject:
isotope labeling; potassium nitrate; promoter regions; plant proteins; small interfering RNA; radionuclides; nutrient uptake; gene expression; membrane proteins; plasma membrane; gene expression regulation; ammonium nitrate; Arabidopsis thaliana; messenger RNA; photorespiration; transcription (genetics); plant tissues; green fluorescent protein; nitrogen; Saccharomyces cerevisiae; reporter genes; chemical constituents of plants; plant morphology; beta-glucuronidase
Abstract:
... AtAMT2 is an ammonium transporter that is only distantly related to the five members of the AtAMT1 family of high-affinity ammonium transporters in Arabidopsis. The short-lived radioactive ion 13NH4+ was used to show that AtAMT2, expressed in yeast (Saccharomyces cerevisiae), is a high-affinity transporter with a K(m) for ammonium of about 20 micromolar. Changes in external pH between 5.0 and 7.5 ...
PubMed:
12481062
PubMed Central:
PMC166690

32. Characterization of P69E and P69F, two differentially regulated gene encoding new members of the subtilisin-like proteinase family from tomato plants

Author:
Jorda, L.; Conejero, V.; Vera, P.
Source:
Plant physiology 2000 v.122 no.1 pp. 67-73
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; messenger RNA; serine proteinases; transgenic plants; amino acid sequences; gene expression; genes; promoter regions; beta-glucuronidase; Solanum lycopersicum var. lycopersicum
Abstract:
... Subtilisin-like proteins represent an ancient family of serine proteases that are extremely widespread in living organisms. We report here the structure and genomic organization of two new transcriptionally active genes encoding proteins thatbelong to the P69 family of subtilisin-like proteases from tomato (Lycopersicon esculentum) plants. The two new members, P69E and P69F, are organized in a clu ...

33. Characterization of a tobacco bright yellow 2 cell line expressing the tetracycline repressor at a high level for strict regulation of transgene expression

Author:
David, K.M.; Perrot-Rechenmann, C.
Source:
Plant physiology 2001 v.125 no.4 pp. 1548-1553
ISSN:
0032-0889
Subject:
tetracycline; green fluorescent protein; animal proteins; transgenic plants; gene expression; Scyphozoa; Nicotiana tabacum; reporter genes; cell lines; enzyme activity; promoter regions; beta-glucuronidase
Abstract:
... Manipulating the expression of a transgene in transient and stable transformed cells is a requirement for many functional analyses. We have investigated the use of the tetracycline-dependent gene expression system developed by Gatz et al. (1992) in tobacco (Nicotiana tabacum L. cv Bright Yellow 2 [BY2]) cells, the most widely used plant cell culture. We have selected a BY2 cell line, named BY2-tet ...
PubMed:
11299335
PubMed Central:
PMC1539379

34. Cinnamyl alcohol dehydrogenase: identification of new sites of promoter activity in transgenic poplar

Author:
Hawkins, S.; Samaj, J.; Lauvergeat, V.; Boudet, A.; Grima-Pettenati, J.
Source:
Plant physiology 1997 v.113 no.2 pp. 321-325
ISSN:
0032-0889
Subject:
alcohol dehydrogenase; gene transfer; promoter regions; hybrids; stems; Populus alba; transgenic plants; Eucalyptus gunnii; cinnamic acid; gene expression; genes; Populus tremula; enzyme activity; histochemistry; beta-glucuronidase
Abstract:
... Stem sections from poplar that were stably transformed with a eucalypt cinnamyl alcohol dehydrogenase promoter-beta-glucuronidase construct were prepared by using either a technique routinely used in herbaceous species or a technique designed to take into account the particular anatomy of woody plants. Although both preparation techniques confirmed the pattern of expression previously observed (C. ...

35. Cloning and molecular analyses of a gibberellin 20-oxidase gene expressed specifically in developing seeds of watermelon

Author:
Kang, H.G.; Jun, S.H.; Kim, J.; Kawaide, H.; Kamiya, Y.; An, G.
Source:
Plant physiology 1999 v.121 no.2 pp. 373-382
ISSN:
0032-0889
Subject:
biosynthesis; seeds; complementary DNA; Escherichia coli; immunohistochemistry; recombinant proteins; amino acid sequences; gene expression; oxidoreductases; Citrullus lanatus; oxidation; gibberellins; parthenocarpy; reporter genes; promoter regions; beta-glucuronidase; seed development
Abstract:
... To understand the biosynthesis and functional role of gibberellins (GAs) in developing seeds, we isolated Cv20ox, acDNA clone from watermelon (Citrullus lanatus) that shows significant amino acid homology with GA 20-oxidases. The complementary DNA clone was expressed in Escherichia coli as a fusion protein, which oxidized GA(12) at C-20 to the C(19) compound GA(9), a precursor of bioactive GAs. RN ...
PubMed:
10517828
PubMed Central:
PMC59399

36. Combinatorial interaction of cis elements specifies the expression of the Arabidopsis AtHsp90-1 gene

Author:
Haralampidis, K.; Milioni, D.; Rigas, S.; Hatzopoulos, P.
Source:
Plant physiology 2002 v.129 no.3 pp. 1138-1149
ISSN:
0032-0889
Subject:
seedling growth; seedlings; arsenites; pollen; promoter regions; plant proteins; meristems; heat stress; gene expression; gene expression regulation; Arabidopsis thaliana; sequence analysis; messenger RNA; transcription (genetics); enzyme activity; heat shock proteins; transgenic plants; stress response; flowers; reporter genes; chemical constituents of plants; plant morphology; beta-glucuronidase
Abstract:
... The promoter region of the Arabidopsis AtHsp90-1 gene is congested with heat shock elements and stress response elements, as well as with other potential transcriptional binding sites (activating protein 1, CCAAT/enhancer-binding protein element, and metal regulatory element). To determine how the expression of this bona fide AtHsp90-1 gene is regulated, a comprehensive quantitative and qualitativ ...
PubMed:
12114568
PubMed Central:
PMC166508

37. Controlled cytokinin production in transgenic tobacco using a copper-inducible promoter

Author:
McKenzie, M.J.; Mett, V.; Reynolds, P.H.S.; Jameson, P.E.
Source:
Plant physiology 1998 v.116 no.3 pp. 969-977
ISSN:
0032-0889
Subject:
promoter regions; plant development; buds; biosynthesis; phenotype; copper; transgenic plants; developmental stages; gene expression; apical dominance; cytokinins; Nicotiana tabacum; reporter genes; enzyme activity; plant morphology; roots; beta-glucuronidase; leaves; tissue culture
Abstract:
... The cytokinin group of plant hormones regulates aspects of plant growth and development, including the release of lateral buds from apical dominance and the delay of senescence. In this work the native promoter of a cytokinin synthase gene (ipt) was removed and replaced with a Cu-controllable promoter. Tobacco (Nicotiana tabacum L. cv tabacum) transformed with this Cu-inducible ipt gene (Cu-ipt) w ...

38. Cytokinin oxidase gene expression in maize is localized to the vasculature, and is induced by cytokinins, abscisic acid, and abiotic stress

Author:
Brugiere, N.; Jiao, S.; Hantke, S.; Zinselmeier, C.; Roessler, J.A.; Niu, X.O.; Jones, R.J.; Habben, J.E.
Source:
Plant physiology 2003 v.132 no.3 pp. 1228-1240
ISSN:
0032-0889
Subject:
alleles; seedlings; roots; plant proteins; coleoptiles; dose response; open reading frames; tissue distribution; water stress; gene expression; heat stress; isopentenyladenine; Zea mays; plant development; messenger RNA; corn; vascular bundles; grain crops; transgenic plants; oxidoreductases; cytokinins; reporter genes; abscisic acid; beta-glucuronidase
DOI:
10.1104/pp.102.017707
PubMed:
12857805
PubMed Central:
PMC167063

39. DNA sequences that activate isocitrate lyase gene expression during late embryogenesis and during postgerminative growth

Author:
Zhang, J.Z.; Santes, C.M.; Engel, M.L.; Gasser, C.S.; Harada, J.J.
Source:
Plant physiology 1996 v.110 no.4 pp. 1069-1079
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; seed germination; metabolism; genetic code; beta-glucuronidase; transgenic plants; DNA; embryo (plant); seedlings; transcription (genetics); gene expression; histochemistry; nucleotide sequences; embryogenesis; plant morphology; glyoxysomes; enzyme activity; isocitrate lyase
Abstract:
... We analyzed DNA sequences that regulate the expression of an isocitrate lyase gene from Brassica napus L. during late embryogenesis and during postgerminative growth to determine whether glyoxysomal function is induced by a common mechanism at different developmental stages. beta-Glucuronidase constructs were used both in transient expression assays in B. napus and in transgenic Arabidopsis thalia ...
PubMed:
8934622
PubMed Central:
PMC160883

40. Dehydration-stress-regulated transgene expression in stably transformed rice plants

Author:
Su, J.; Shen, Q.; Ho, T.H.D.; Wu, R.
Source:
Plant physiology 1998 v.117 no.3 pp. 913-922
ISSN:
0032-0889
Subject:
Oryza sativa; Hordeum vulgare; cell suspension culture; roots; leaves; protoplasts; genetic transformation; plasmids; genes; gene transfer; chromosome mapping; transgenic plants; gene expression; abscisic acid; beta-glucuronidase; enzyme activity; aleurone cells; promoter regions; stress response
Abstract:
... To confer abscisic acid (ABA) and/or stress-inducible gene expression, an ABA-response complex (ABRC1) from the barley (Hordeum vulgare L.) HVA22 gene was fused to four different lengths of the 5' region from the rice (Oryza sativa L.) Act1 gene. Transient assay of beta-glucuronidase (GUS) activity in barley aleurone cells shows that, coupled with ABRC1, the shortest minimal promoter (Act1-100P) g ...

41. Developmental and stress regulation of RCI2A and RCI2B, two cold-inducible genes of Arabidopsis encoding highly conserved hydrophobic proteins

Author:
Medina, J.; Catala, R.; Salinas, J.
Source:
Plant physiology 2001 v.125 no.4 pp. 1655-1666
ISSN:
0032-0889
Subject:
guard cells; hydrophobicity; pollen; promoter regions; plant proteins; water stress; gene expression; Arabidopsis thaliana; amino acid sequences; transcription (genetics); vascular bundles; seed germination; salinity; cold stress; reporter genes; abscisic acid; beta-glucuronidase; seed development
Abstract:
... The capability of most higher plants to tolerate environmental conditions strongly depends on their developmental stage. In addition, environmental factors have pleiotropic effects on many developmental processes. The interaction between plant development and environmental conditions implies that some genes must be regulated by both environmental factors and developmental cues. To understand their ...
PubMed:
11299347
PubMed Central:
PMC88823

42. Developmental expression of tomato heat-shock cognate protein 80

Author:
Koning, A.J.; Rose, R.; Comai, L.
Source:
Plant physiology 1992 v.100 no.2 pp. 801-811
ISSN:
0032-0889
Subject:
heat shock proteins; genetic transformation; messenger RNA; plant development; recombinant DNA; structural genes; transgenic plants; amino acid sequences; gene expression; histochemistry; reporter genes; introns; promoter regions; beta-glucuronidase; Solanum lycopersicum var. lycopersicum
Abstract:
... Heat-shock protein 80 (HSP80) is a major heat-shock protein induced in yeast and animals both by heat shock and by specific developmental events. In plants, a heat-shock-induced HSP80 cDNA has been described, although no information concerning developmental regulation of HSP80 genes is available. We have characterized a tomato (Lycopersicon esculentum) gene encoding a typical HSP80 protein. This g ...
DOI:
10.1104/pp.100.2.801
PubMed:
16653061

43. Developmental regulation of pyrroline-5-carboxylate reductase gene expression in Arabidopsis

Author:
Hua, X.J.; Cotte, B. van de.; Montagu, M. van.; Verbruggen, N.
Source:
Plant physiology 1997 v.114 no.4 pp. 1215-1224
ISSN:
0032-0889
Subject:
guard cells; root tips; osmotic pressure; developmental stages; genes; shoot meristems; pollen; promoter regions; proline; gene expression; Arabidopsis thaliana; ovules; plant development; quantitative analysis; seeds; genetic code; transcription (genetics); enzyme activity; histochemistry; carboxylic acids; gene transfer; biosynthesis; transgenic plants; flowers; oxidoreductases; beta-glucuronidase
Abstract:
... At-P5R, a gene encoding the last enzyme of the proline (Pro) biosynthetic pathway in Arabidopsis thaliana, is developmentally regulated. To characterize the cis elements responsible for this developmental regulation, a series of 5' deletions of the At-P5R promoter were transcriptionally fused to a beta-glucuronidase (GUS)-coding region and transformed into Arabidopsis. The complete promoter of At- ...
PubMed:
9276946
PubMed Central:
PMC158414

44. Different phototransduction kinetics of phytochrome A and phytochrome B in Arabidopsis thaliana

Author:
Casal, J.J.; Cerdan, P.D.; Staneloni, R.J.; Cattaneo, L.
Source:
Plant physiology 1998 v.116 no.4 pp. 1533-1538
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; seedlings; photosystem II; phytochrome; kinetics; hypocotyls; reporter genes; gene expression; far-red light; red light; genetic code; beta-glucuronidase; enzyme activity; light harvesting complex; promoter regions; etiolation
Abstract:
... The kinetics of phototransduction of phytochrome A (phyA) and phytochrome B (phyB) were compared in etiolated Arabidopsis thaliana seedlings. The responses of hypocotyl growth, cotyledon unfolding, and expression of a light-harvesting chlorophyll a/b-binding protein of the photosystem II gene promoter fused to the coding region of beta-glucuronidase (used as a reporter enzyme) were mediated by phy ...
PubMed:
9536072
PubMed Central:
PMC35062

45. Differential Expression of the Arabidopsis Cytochrome c Genes Cytc-1 and Cytc-2. Evidence for the Involvement of TCP-Domain Protein-Binding Elements in Anther- and Meristem-Specific Expression of the Cytc-1 Gene

Author:
Welchen, Elina; Gonzalez, Daniel H.
Source:
Plant physiology 2005 v.139 no.1 pp. 88-100
ISSN:
0032-0889
Subject:
protein binding; plant extracts; benzyladenine; promoter regions; plant proteins; recombinant fusion proteins; meristems; tissue distribution; gene expression; mitochondria; gene expression regulation; Arabidopsis thaliana; anthers; sucrose; mutagenesis; cell nucleus; plant genetics; cytochrome c; cytokinins; cauliflower; reporter genes; DNA-binding proteins; Brassica oleracea var. botrytis; electron transport chain; beta-glucuronidase
Abstract:
... The promoters of the Arabidopsis (Arabidopsis thaliana) cytochrome c genes, Cytc-1 and Cytc-2, were analyzed using plants transformed with fusions to the [beta]-glucuronidase coding sequence. Histochemical staining of plants indicated that the Cytc-1 promoter directs preferential expression in root and shoot meristems and in anthers. In turn, plants transformed with the Cytc-2 promoter fusions sho ...
DOI:
10.1104/pp.105.065920
PubMed:
16113211
PubMed Central:
PMC1203360

46. Differential patterns of expression of the Arabidopsis PHYB, PHYD, and PHYE phytochrome genes

Author:
Goosey, L.; Palecanda, L.; Sharrock, R.A.
Source:
Plant physiology 1997 v.115 no.3 pp. 959-969
ISSN:
0032-0889
Subject:
root tips; seedling growth; developmental stages; promoter regions; photoreceptors; phenotype; gene expression; phytochrome; leaves; Arabidopsis thaliana; morphogenesis; amino acid sequences; enzyme activity; apoproteins; transgenic plants; flowers; mutants; reporter genes; genetic variation; plant morphology; beta-glucuronidase; light
Abstract:
... The Arabidopsis thaliana phyB, phyD, and phyE phytochrome apoproteins show higher amino acid sequence similarity to each other than to phyA or phyC, they are the most recently evolved members of this photoreceptor family, and they may interact in regulating photomorphogenesis. The expression patterns of translational fusions of the 5' upstream regions of the PHYB, PHYD, and PHYE genes to the beta- ...
PubMed:
9390432
PubMed Central:
PMC158559

47. Diphtheria toxin-mediated cell ablation reveals interregional communication during arabidopsis seed development

Author:
Weijers, D.; van Hamburg, J.P.; van Rijn, E.; Hooykaas, P.J.J.; Offringa, R.
Source:
Plant physiology 2003 v.133 no.4 pp. 1882-1892
ISSN:
0032-0889
Subject:
Corynebacterium diphtheriae; bacterial proteins; transgenes; gene expression; Arabidopsis thaliana; seed development; endosperm; testa; embryo (plant); green fluorescent protein; beta-glucuronidase; reporter genes; transcriptional activation; cell communication
Abstract:
... Fertilization of the female gametophyte in angiosperm plants initiates a process of coordinated development of embryo, endosperm, and seed coat that ensures the production of a viable seed. Mutant analysis has suggested that communication between the endosperm and the seed coat is an important determinant in this process. In addition, cell groups within the embryo, derived from the apical and from ...
DOI:
10.1104/pp.103.030692
PubMed:
14605218
PubMed Central:
PMC300741

48. An Egg Apparatus-Specific Enhancer of Arabidopsis, Identified by Enhancer Detection

Author:
Yang, Wei; Jefferson, Richard A.; Huttner, Eric; Moore, James M.; Gagliano, Wendy B.; Grossniklaus, Ueli
Source:
Plant physiology 2005 v.139 no.3 pp. 1421-1432
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; enhancer elements; plant genetics; gametophytes; plant reproduction; reporter genes; gene expression regulation; peroxidase; beta-glucuronidase
Abstract:
... Despite a central role in angiosperm reproduction, few gametophyte-specific genes and promoters have been isolated, particularly for the inaccessible female gametophyte (embryo sac). Using the Ds-based enhancer-detector line ET253, we have cloned an egg apparatus-specific enhancer (EASE) from Arabidopsis (Arabidopsis thaliana). The genomic region flanking the Ds insertion site was further analyzed ...
DOI:
10.1104/pp.105.068262
PubMed:
16258010
PubMed Central:
PMC1283777

49. Enhancer trap expression patterns provide a novel teaching resource

Author:
Geisler, M.; Jablonska, B.; Springer, P.S.
Source:
Plant physiology 2002 v.130 no.4 pp. 1747-1753
ISSN:
0032-0889
Subject:
Arabidopsis thaliana; plant development; transgenic plants; gene expression regulation; educational materials; gene expression; reporter genes; genetic variation; plant morphology; roots; beta-glucuronidase; leaves
Abstract:
... A collection of Arabidopsis enhancer trap transposants has been identified for use as a teaching tool. This collection serves to assist students in understanding the patterning and organization of plant tissues and cells, and will be useful in plant anatomy, morphology, and developmental biology courses. Each transposant exhibits reporter gene expression in a specific tissue, cell type, or domain, ...
PubMed:
12481057
PubMed Central:
PMC1540270

50. Ethanol vapor is an efficient inducer of the alc gene expression system in model and crop plant species

Author:
Sweetman, J.P.; Chu, C.; Qu, N.; Greenland, A.J.; Sonnewald, U.; Jepson, I.
Source:
Plant physiology 2002 v.129 no.3 pp. 943-948
ISSN:
0032-0889
Subject:
alcohol dehydrogenase; chemical constituents of plants; transgenic plants; recombinant proteins; starch crops; Solanum tuberosum; gene expression; potatoes; Nicotiana tabacum; leaves; Brassica napus; reporter genes; vapors; plant morphology; ethanol; beta-glucuronidase; tobacco; plant proteins
Abstract:
... We have demonstrated that low concentrations of ethanol vapor efficiently induce the alc gene expression system in tobacco (Nicotiana tabacum cv Samsun NN), potato (Solanum tuberosum cv Solara), and oilseed rape (Brassica napus cv Westar). For many situations, this may be the preferred method of induction because it avoids direct application of comparatively high concentrations of an ethanol solut ...
PubMed:
12114549
PubMed Central:
PMC1540238