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Preparative biochemistry & biotechnology
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absorbance
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- Author:
- Ekant Tamboli; Aditi Bhatnagar; Abha Mishra
- Source:
- Preparative biochemistry & biotechnology 2018 v.48 no.8 pp. 693-699
- ISSN:
- 1532-2297
- Subject:
- Pleurotus ostreatus; absorbance; acetone; alpha-amylase; alpha-glucosidase; bioactive compounds; carbohydrate metabolism; chloroform; enzyme inhibition; enzyme inhibitors; flavanols; gallic acid; glycoproteins; high performance liquid chromatography; inhibitory concentration 50; methanol; mushrooms; mycelium
- Abstract:
- ... The α-Amylase and α-glucosidase are two main enzymes involved in carbohydrate metabolism. This study was aimed at detecting alpha-amylase inhibitory activity from edible mushroom mycelia. Oyster mushroom was collected from a natural source, from Indian Institute of Technology (Banaras Hindu University) campus and was maintained in vitro in mycelial form. Chloroform, acetone, methanol, and water we ...
- DOI:
- 10.1080/10826068.2018.1487849
- https://doi.org/10.1080/10826068.2018.1487849
- Author:
- Fatemeh Khodabakhsh; Mohammad Farid Zia; Fatemeh Moazen; Mohammad Rabbani; Hamid Mir Mohammad Sadeghi
- Source:
- Preparative biochemistry & biotechnology 2013 v.43 no.7 pp. 613-623
- ISSN:
- 1532-2297
- Subject:
- Escherichia coli; Western blotting; absorbance; biotechnology; complementary DNA; plasmids; polyacrylamide gel electrophoresis; polymerase chain reaction; prokaryotic cells; protein synthesis; sodium dodecyl sulfate; t-plasminogen activator
- Abstract:
- ... Reteplase is the recombinant type of tissue plasminogen activator variant. In this study, preplasmic and cytoplasmic (as inclusion body: IBs) production and activity of recombinant reteplase in E. coli were investigated and compared using a pET system (pET22b and pET15b). The cDNA of reteplase was cloned by polymerase chain reaction (PCR) amplification, sequenced, inserted into the vector pET 22b ...
- DOI:
- 10.1080/10826068.2013.764896
- https://doi.org/10.1080/10826068.2013.764896
- Author:
- Shabina Yeasmin; Chul-Hwan Kim; Shah Md. Asraful Islam; Ji-Young Lee
- Source:
- Preparative biochemistry & biotechnology 2016 v.46 no.3 pp. 229-237
- ISSN:
- 1532-2297
- Subject:
- Fourier transform infrared spectroscopy; absorbance; alkali treatment; atomic force microscopy; biomass; ethanol; ethanol fermentation; ethanol production; glucose; hydrolysis; lignin; rice straw; saccharification; scanning electron microscopy; sodium hydroxide
- Abstract:
- ... The efficacy of different concentrations of NaOH (0.25%, 0.50%, 0.75%, and 1.00%) for the pretreatment of rice straw in solid and powder state in enzymatic saccharification and fermentation for the production of bioethanol was evaluated. A greater amount of biomass was recovered through solid-state pretreatment (3.74 g) from 5 g of rice straw. The highest increase in the volume of rice straw powde ...
- DOI:
- 10.1080/10826068.2015.1015563
- https://doi.org/10.1080/10826068.2015.1015563
- Author:
- Herng C. Leow; Katja Fischer; Yee C. Leow; Katleen Braet; Qin Cheng; James McCarthy
- Source:
- Preparative biochemistry & biotechnology 2019 v.49 no.4 pp. 315-327
- ISSN:
- 1532-2297
- Subject:
- Escherichia coli; absorbance; antigens; biomarkers; biotechnology; cysteine; disulfide bonds; heat stability; malaria; mice; monoclonal antibodies; sharks; signal peptide; solubility
- Abstract:
- ... Shark variable new antigen receptors (VNARs) are known to possess excellent heat-stability, and the long complementarity determining region 3 (CDR3) has permitted it to penetrate into the cleft region of antigens. The number of cysteine (Cys) residues contained within VNAR is greater than in conventional antibodies, entailing disulfide bond formation in both the inter- or intra-loop regions is req ...
- DOI:
- 10.1080/10826068.2019.1566145
- https://doi.org/10.1080/10826068.2019.1566145
- Author:
- Magdalena Ong; Clarence M. Ongkudon; Clemente Michael Vui Ling Wong
- Source:
- Preparative biochemistry & biotechnology 2016 v.46 no.7 pp. 657-665
- ISSN:
- 1532-2297
- Subject:
- Pedobacter; absorbance; bioremediation; cold zones; cost effectiveness; culture media; dipotassium hydrogen phosphate; experimental design; glucose; magnesium sulfate; medicine; nutrients; prices; response surface methodology; screening; sodium chloride; temperature; tryptones; yeast extract
- Abstract:
- ... Pedobacter cryoconitis BG5 are psychrophiles isolated from the cold environment and capable of proliferating and growing well at low temperature regime. Their cellular products have found a broad spectrum of applications, including in food, medicine, and bioremediation. Therefore, it is imperative to develop a high-cell density cultivation strategy coupled with optimized growth medium for P. cryoc ...
- DOI:
- 10.1080/10826068.2015.1135447
- https://doi.org/10.1080/10826068.2015.1135447
- Author:
- Michele R. Spier; Francieli B. Siepmann; Larissa Staack; Priscila Z. Souza; Vikas Kumar; Adriane B. P. Medeiros; Carlos R. Soccol
- Source:
- Preparative biochemistry & biotechnology 2016 v.46 no.7 pp. 725-733
- ISSN:
- 1532-2297
- Subject:
- EDTA (chelating agent); absorbance; chelation; enzyme activity; enzyme stability; feed additives; feed industry; growth retardation; mannitol; methylparaben; microbial growth; monitoring; mushrooms; phytases; polyethylene glycol; proteinase inhibitors; proteolysis; screening; serine proteinases; sodium chloride; sodium citrate; solid state culture; storage time
- Abstract:
- ... The development of stable enzymes is a key issue in both the food and feed industries. Consequently, the aim of the current study is to evaluate the impact of various additives (sodium chloride, sodium citrate, mannitol, methylparaben, polyethylene glycol 3350, ethylenediaminetetraacetic acid disodium salt, and a serine protease inhibitor) on the stability of a mushroom phytase produced by solid-s ...
- DOI:
- 10.1080/10826068.2015.1135456
- https://doi.org/10.1080/10826068.2015.1135456
- Author:
- Mohamed Boumaiza; Khaled Trabelsi; Zeineb Choucha; Ines Akrouti; Serena Leone; Delia Picone; Héla Kallel
- Source:
- Preparative biochemistry & biotechnology 2021 v.51 no.6 pp. 562-569
- ISSN:
- 1532-2297
- Subject:
- Baculoviridae; Escherichia coli; Orthohepevirus A; Western blotting; absorbance; affinity chromatography; biotechnology; coat proteins; digestion; insects; public health; structural proteins; thrombin; viruses; zoonoses
- Abstract:
- ... Hepatitis E virus (HEV) is a nonenveloped virus causing an emerging zoonotic disease posing a severe threat to the public health in the world, especially to pregnant women. In this study, a truncated form (aa 368–606) of the open reading frame 2 of the capsid protein (tORF2-HEV), a major structural protein of HEV, was expressed in Escherichia coli. This work characterizes for the first time, the f ...
- DOI:
- 10.1080/10826068.2020.1836656
- https://doi.org/10.1080/10826068.2020.1836656
- Author:
- Padiglia, A.; Medda, R.; Cruciani, E.; Lorrai, A.; Floris, G.
- Source:
- Preparative biochemistry & biotechnology 1996 v.26 no.2 pp. 135-142
- ISSN:
- 1082-6068
- Subject:
- Oryza sativa; seeds; superoxide dismutase; copper; zinc; metal ions; molecular weight; enzyme activity; isoelectric point; temperature; heat stability; energy; isozymes; absorbance; spectral analysis; protein subunits; activation energy
- Abstract:
- ... Superoxide dismutase has been purified to homogeneity from Oryza sativa germinated seeds growth in the dark. The purified enzyme contained two electrophoretically distinct bands on continuous gel electrophoresis or analytical gel electrofocusing. SDS-PAGE showed a single band of an Mr 15 000 while gel chromatography on Sephadex G 100 showed a single peak of an Mr 32 000. It contained two Cu and tw ...
- DOI:
- 10.1080/10826069608000059
- PubMed:
- 8784923
- https://doi.org/10.1080/10826069608000059
- Author:
- Alireza Moosavi Zenooz; Farzin Zokaee Ashtiani; Reza Ranjbar; Najvan Javadi
- Source:
- Preparative biochemistry & biotechnology 2016 v.46 no.6 pp. 567-574
- ISSN:
- 1532-2297
- Subject:
- Synechococcus; absorbance; autotrophs; biomass production; cell growth; light intensity; models; photobioreactors; photoinhibition
- Abstract:
- ... Synechococcus sp. (PTCC 6021), a cyanobacterium species, was cultivated in an internally illuminated photobioreactor. The reactor was designed to achieve a monoseptic cultivation of the species. The goal was to study the growth–irradiance behavior of Synechococcus sp. (PTCC 6021). To accomplish this, different initial light irradiances were implemented inside the photobioreactor and the growth of ...
- DOI:
- 10.1080/10826068.2015.1084931
- https://doi.org/10.1080/10826068.2015.1084931