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You searched for: Journal Proceedings of the National Academy of Sciences of the United States of America Remove constraint Journal: Proceedings of the National Academy of Sciences of the United States of America Publication Year 2013 Remove constraint Publication Year: 2013 Source 2013 v.110 no.28 Remove constraint Source: 2013 v.110 no.28 Subject models Remove constraint Subject: models Subject gene expression Remove constraint Subject: gene expression Subject RNA libraries Remove constraint Subject: RNA libraries
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1. Tuning gene expression with synthetic upstream open reading frames

Author:
Ferreira, Joshua P.; Overton, K. Wesley; Wang, Clifford L.
Source:
Proceedings of the National Academy of Sciences of the United States of America 2013 v.110 no.28 pp. 11284-11289
ISSN:
0027-8424
Subject:
RNA; RNA libraries; engineering; gene expression; mammals; models; open reading frames; protein synthesis; recombinant proteins; ribosomes; start codon
Abstract:
... We engineered short ORFs and used them to control the expression level of recombinant proteins. These short ORFs, encoding a two-amino acid peptide, were placed upstream of an ORF encoding a protein of interest. Insertion of these upstream ORFs (uORFs) resulted in suppression of protein expression. By varying the base sequence preceding the uORF, we sought to vary the translation initiation rate o ...
DOI:
10.1073/pnas.1305590110
PubMed:
23798422
PubMed Central:
PMC3710870