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- Wolucka, B.A.; Persiau, G.; Doorsselaere, J. van.; Davey, M.W.; Demol, H.; Vandekerckhove, J.; Montagu, M. van.; Zabeau, M.; Boerjan, W.
- Proceedings of the National Academy of Sciences of the United States of America 2001 v.98 no.26 pp. 14843-14848
- Arabidopsis thaliana; biosynthesis; molecular weight; amino acid sequences; isomerases; exons; enzyme activity; introns; ascorbic acid
- ... The first step in the biosynthetic pathway of vitamin C in plants is the formation, at the level of sugar nucleotide, of L-galactosyl residues, catalyzed by a largely unknown GDP-D-mannose 3",5"-epimerase. By using combined conventional biochemical and mass spectrometry methods, we obtained a highly purified preparation of GDP-D-mannose 3",5"-epimerase from an Arabidopsis thaliana cell suspension. ...
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