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- Juarez, K.; Flores, H.; Davila, S.; Olvera, L.; Gonzalez, V.; Morett, E.
- Proceedings of the National Academy of Sciences of the United States of America 2000 v.97 no.7 pp. 3314-3318
- recombinant DNA, etc ; transcription factors; transcriptional activation; mutagenesis; Ensifer meliloti; beta-galactosidase; transcription (genetics); Bradyrhizobium japonicum; reporter genes; oxygen; DNA-binding domains; promoter regions; Show all 12 Subjects
- ... Although the concept of domain merging and shuffling as a major force in protein evolution is well established, it has been difficult to demonstrate how domains coadapt. Here we show evidence of coevolution of the Sinorhizobium meliloti NifA (SmNifA) domains. We found that, because of the lack of a conserved glycine in its DNA-binding domain, this transactivator protein interacts weekly with enhan ...