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You searched for: Journal Proceedings of the National Academy of Sciences of the United States of America Remove constraint Journal: Proceedings of the National Academy of Sciences of the United States of America Publication Year 1995 Remove constraint Publication Year: 1995 Source 1995 v.92 no.19 Remove constraint Source: 1995 v.92 no.19
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1. A chloroplast lipoxygenase is required for wound-induced jasmonic acid accumulation in Arabidopsis

Author:
Bell, E.; Creelman, R.A.; Mullet, J.E.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1995 v.92 no.19 pp. 8675-8679
ISSN:
0027-8424
Subject:
Arabidopsis thaliana; messenger RNA; biosynthesis; complementary DNA; structural genes; jasmonic acid; transgenic plants; linoleate 13S-lipoxygenase; gene expression; mechanical damage; leaves; abiotic stress; Pisum sativum; enzyme activity; chloroplasts
Abstract:
... Plant lipoxygenases are thought to be involved in the biosynthesis of lipid-derived signaling molecules. The potential involvement of a specific Arabidopsis thaliana lipoxygenase isozyme, LOX2, in the biosynthesis of the plant growth regulators jasmonic acid (JA) and abscisic acid was investigated. Our characterization of LOX2 indicates that the protein is targeted to chloroplasts. The physiologic ...
DOI:
10.1073/pnas.92.19.8675
PubMed:
7567995
PubMed Central:
PMC41029

2. A trace component of ginseng that inhibits Ca2+ channels through a pertussis toxin-sensitive G protein

Author:
Nah, S.Y.; Park, H.J.; McCleskey, E.W.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1995 v.92 no.19 pp. 8739-8743
ISSN:
0027-8424
Subject:
neurophysiology; Panax pseudoginseng; medicinal properties; guanosine triphosphate; ion transport; calcium; binding proteins; saponins; electrophysiology; plasma membrane; rats; cell lines; sensory neurons; inorganic ions
Abstract:
... A crude extract from ginseng root inhibits high threshold, voltage-dependent Ca2+ channels through an unknown receptor linked to a pertussis toxin-sensitive G protein. We now have found the particular compound that seems responsible for the effect: it is a saponin, called ginsenoside Rf (Rf), that is present in only trace amounts within ginseng. At saturating concentrations, Rf rapidly and reversi ...
DOI:
10.1073/pnas.92.19.8739
PubMed:
7568008
PubMed Central:
PMC41042

3. Cutting activates a 46-kilodalton kinase in plants

Author:
Usami, S.; Banno, H.; Nishihama, R.; Machida, Y.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1995 v.92 no.19 pp. 8660-8664
ISSN:
0027-8424
Subject:
threonine; serine; cutting; tyrosine; Nicotiana tabacum; mechanical damage; abiotic stress; phosphorylation; leaves
Abstract:
... Using SDS/polyacrylamide gels that contained myelin basic protein, we identified a 46-kDa protein kinase in tobacco that is transiently activated by cutting. Although the activity of the kinase was rarely detectable in mature leaves, marked activity became apparent within several minutes after isolation of leaf discs and subsided within 30 min. In the presence of cycloheximide (CHX), the kinase ac ...
DOI:
10.1073/pnas.92.19.8660

4. Cytochrome c oxidase in Neurospora crassa contains myristic acid covalently linked to subunit 1

Author:
Vassilev, A.O.; Plesofsky-Vig, N.; Brambl, R.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1995 v.92 no.19 pp. 8680-8684
ISSN:
0027-8424
Subject:
Neurospora crassa; cytochrome-c oxidase; myristic acid; chemical reactions; lysine
Abstract:
... Radiolabel from [3H]myristic acid was incorporated by Neurospora crassa into the core catalytic subunit 1 of cytochrome c oxidase (EC 1.9.3.1), as indicated by immunoprecipitation. This modification of the subunit, which was specific for myristic acid, represents an uncommon type of myristoylation through an amide linkage at an internal lysine, rather than an N-terminal glycine. The [3H]myristate, ...
DOI:
10.1073/pnas.92.19.8680
PubMed:
7567996
PubMed Central:
PMC41030

5. Elongation factor 1 alpha concentration is highly correlated with the lysine content of maize endosperm

Author:
Habben, J.E.; Moro, G.L.; Hunter, B.G.; Hamaker, B.R.; Larkins, B.A.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1995 v.92 no.19 pp. 8640-8644
ISSN:
0027-8424
Subject:
correlation; Zea mays; lysine; barley; Hordeum vulgare; binding proteins; ribosomes; endosperm; Sorghum bicolor; protein composition
Abstract:
... Lysine is the most limiting essential amino acid in cereals, and for many years plant breeders have attempted to increase its concentration to improve the nutritional quality of these grains. The opaque2 mutation in maize doubles the lysine content in the endosperm, but the mechanism by which this occurs is unknown. We show that elongation factor 1 alpha (EF-1 alpha) is overexpressed in opaque2 en ...
DOI:
10.1073/pnas.92.19.8640
PubMed:
7567989
PubMed Central:
PMC41022

6. Expression of a plant viral polycistronic mRNA in yeast, Saccharomyces cerevisiae, mediated by a plant virus translational transactivator

Author:
Sha, Y.; Broglio, E.P.; Cannon, J.F.; Schoelz, J.E.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1995 v.92 no.19 pp. 8911-8915
ISSN:
0027-8424
Subject:
Cauliflower mosaic virus; Saccharomyces cerevisiae; viral proteins; structural genes; gene expression; messenger RNA; genetic transformation; plasmid vectors; recombinant DNA; chloramphenicol acetyltransferase; reporter genes; enzyme activity; promoter regions
Abstract:
... We demonstrate that the cauliflower mosaic virus (CaMV) gene TQ product can transactivate the expression of a reporter gene in bakers' yeast, Saccharomyces cerevisiae. The gene P7 coding sequence was placed under the control of the galactose-inducible promoter GAL1, which is present in the yeast shuttle vector pYES2, to create plasmid JS169. We also created a chloramphenicol acetyltransferase (CAT ...
DOI:
10.1073/pnas.92.19.8911
PubMed:
7568042
PubMed Central:
PMC41077

7. Functional complementation of the ste6 gene of Saccharomyces cerevisiae with the pfmdr 1 gene of Plasmodium falciparum

Author:
Yolkman, S.K.; Cowman, A.F.; Wirth, D.F.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1995 v.92 no.19 pp. 8921-8925
ISSN:
0027-8424
Subject:
sexual reproduction; mating behavior; Saccharomyces cerevisiae; Plasmodium falciparum; structural genes; binding proteins; mutants; drug resistance; chloroquine; protein transport; pheromones; genetic transformation; mutation; genetic complementation
Abstract:
... The pfmdr1 gene has been associated with; drug-resistant phenotype in Plasmodium falciparum, and over expression of pfmdr1 has been associated with mefloquine and halofantrine-resistant parasites, but little is known about the functional role of pfmdr1 in this process. Here, we demonstrate that the pfmdr1 gene expressed in a heterologous yeast system functions as a transport molecule and complemen ...
DOI:
10.1073/pnas.92.19.8921
PubMed:
7568044
PubMed Central:
PMC41079

8. High-affinity RNA-binding domains of alfalfa mosaic virus coat protein are not required for coat protein-mediated resistance

Author:
Yusibov, V.; Lesch-Fires, L.S.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1995 v.92 no.19 pp. 8980-8984
ISSN:
0027-8424
Subject:
coat proteins; protoplasts; gene transfer; messenger RNA; genetic resistance; structural genes; transgenic plants; mutation; gene expression; Nicotiana tabacum; mutants; disease resistance; binding sites; Alfalfa mosaic virus
Abstract:
... A virus-based vector was used for the transient expression of the alfalfa mosaic virus coat protein (CP) gene in protoplasts and plants. The accumulation of wild-type CP conferred strong protection against subsequent alfalfa mosaic virus infection, enabling the efficacy of CP mutants to be determined without developing transgenic plants. Expression of the CP mRNA alone without CP accumulation conf ...
DOI:
10.1073/pnas.92.19.8980
PubMed:
7568056
PubMed Central:
PMC41091

9. Identification and characterization of putative transposable DNA elements in solanaceous plants and Caenorhabditis elegans

Author:
Oosumi, T.; Garlick, B.; Belknap, W.R.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1995 v.92 no.19 pp. 8886-8890
ISSN:
0027-8424
Subject:
promoter regions; structural genes; sequence alignment; polygalacturonase; Solanum tuberosum; genetic recombination; transposons; Caenorhabditis elegans; Solanum lycopersicum var. lycopersicum
Abstract:
... Several families of putative transposable elements (TrEs) in both solanaceous plants and Caenorhabditis elegans have been identified by screening the DNA data base for inverted repeated domains present in multiple copies in the genome. The elements are localized within intron and flanking regions of many genes. These elements consist of two inverted repeats flanking sequences ranging from 5 bp to ...
DOI:
10.1073/pnas.92.19.8886
PubMed:
7568037
PubMed Central:
PMC41072

10. Molecular characterization of PsbW, a nuclear-encoded component of the photosystem II reaction center complex in spinach

Author:
Lorkovic, Z.J.; Schroder, W.P.; Pakrasi, H.B.; Irrgang, K.D.; Herrmann, R.G.; Oelmuller, R.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1995 v.92 no.19 pp. 8930-8934
ISSN:
0027-8424
Subject:
Spinacia oleracea; complementary DNA; plant proteins; photosystem II; nucleotide sequences; amino acid sequences; polypeptides; gene expression; messenger RNA; light; thylakoids; membrane proteins
Abstract:
... The describe the isolation and characterization of cDNAs encoding the precursor polypeptide of the 6.1-kDa polypeptide associated with the reaction center core of the photosystem II complex from spinach. PsbW, the gene encoding this polypeptide, is present in a single copy per haploid genome. The mature polypeptide with 54 amino acid residues is characterized by a hydrophobic transmembrane segment ...
DOI:
10.1073/pnas.92.19.8930
PubMed:
7568046
PubMed Central:
PMC41081

11. Mutational analysis of phytochrome B identifies a small COOH-terminal-domain region critical for regulatory activity

Author:
Wagner, D.; Quail, P.H.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1995 v.92 no.19 pp. 8596-8600
ISSN:
0027-8424
Subject:
Arabidopsis thaliana; plant development; phenotype; hypocotyls; seedlings; ethyl methanesulfonate; missense mutation; mutants; red light; site-directed mutagenesis; phytochrome
Abstract:
... Overexpression of phytochrome B (phyB) in transgenic Arabidopsis results in enhanced deetiolation in red light. To define domains of phyB functionally important for its regulatory activity, we performed chemical mutagenesis of a phyB-overexpressing line and screened for phenotypic revertants in red light. Four phyB-transgene-linked revertants that retain parental levels of full-length, dimeric, an ...
DOI:
10.1073/pnas.92.19.8596
PubMed:
7567981
PubMed Central:
PMC41013

12. Nodulating strains of Rhizobium loti arise through chromosomal symbiotic gene transfer in the environment

Author:
Sullivan, J.T.; Patrick, H.N.; Lowther, W.L.; Scott, D.B.; Ronson, C.W.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1995 v.92 no.19 pp. 8985-8989
ISSN:
0027-8424
Subject:
Southern blotting; genetic transformation; gene transfer; ribosomal RNA; symbiosis; seed inoculation; ribosomal DNA; strain differences; DNA probes; nucleotide sequences; Rhizobium; nodulation; Lotus corniculatus; nucleic acid hybridization; New Zealand
Abstract:
... Rhizobia were isolated from nodules off a stand of Lotus corniculatus established with a single inoculant strain, ICMP3153, 7 years earlier in an area devoid of naturalized Rhizobium loti. The isolates showed diversity in growth rate, Spe I fingerprint of genomic DNA, and hybridization pattern to genomic DNA probes. The 19% of isolates that grew at the same rate as strain ICMP3153 were the only is ...
DOI:
10.1073/pnas.92.19.8985
PubMed:
7568057
PubMed Central:
PMC41092

13. On constructing folding heteropolymers

Author:
Ebeling, M.; Nadler, W.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1995 v.92 no.19 pp. 8798-8802
ISSN:
0027-8424
Subject:
proteins; protein folding; molecular conformation; algorithms
Abstract:
... Simplified models of the protein-folding process have led to valuable insights into the generic properties of the folding of heteropolymers. On the basis of theoretical arguments, Shakhnovich and Gutin [(1993) Proc. Natl. Acad. Sci. USA 90, 7195-7199] have proposed a specific method to generate folding sequences for one of these. Here we present a model of folding in heteropolymers that is compara ...
DOI:
10.1073/pnas.92.19.8798
PubMed:
7568020
PubMed Central:
PMC41054

14. Prediction of protein folding class using global description of amino acid sequence

Author:
Dubchak, I.; Muchnik, I.; Holbrook, S.R.; Kim, S.H.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1995 v.92 no.19 pp. 8700-8704
ISSN:
0027-8424
Subject:
proteins; molecular conformation; amino acid sequences; prediction; computer analysis; hydrophobicity; solvents; neural networks; protein secondary structure
Abstract:
... We present a method for predicting protein folding class based on global protein chain description and a voting process. Selection of the best descriptors was achieved by a computer-simulated neural network trained on a data base consisting of 83 folding classes. Protein-chain descriptors include overall composition, transition, and distribution of amino acid attributes, such as relative hydrophob ...
DOI:
10.1073/pnas.92.19.8700
PubMed:
7568000
PubMed Central:
PMC41034

15. Rat skeletal muscle selenoprotein W: cDNA clone and mRNA modulation by dietary selenium

Author:
Vendeland, S.C.; Beilstein, M.A.; Yeh, J.Y.; Ream, W.; Whanger, P.D.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1995 v.92 no.19 pp. 8749-8753
ISSN:
0027-8424
Subject:
messenger RNA; complementary DNA; skeletal muscle; selenium; amino acid sequences; gene expression; nucleotide sequences; metalloproteins; rats; dietary minerals; selenocysteine
Abstract:
... Rat skeletal muscle selenoprotein W cDNA was isolated and sequenced. The isolation strategy involved design of degenerate PCR primers from reverse translation of a partial peptide sequence. A reverse transcription-coupled PCR product from rat muscle mRNA was used to screen a muscle cDNA library prepared from selenium-supplemented rats. The cDNA sequence confirmed the known protein primary sequence ...
DOI:
10.1073/pnas.92.19.8749
PubMed:
7568010
PubMed Central:
PMC41044

16. Subunit-destablilzing mutations in Drosophila copper/zinc superoxide dismutase: neuropathology and a model of dimer dysequilibrium

Author:
Phillips, J.P.; Tainer, J.A.; Getzoff, E.D.; Boulianne, G.L.; Kirby, K.; Hilliker, A.J.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1995 v.92 no.19 pp. 8574-8578
ISSN:
0027-8424
Subject:
Drosophila melanogaster; mutagenesis; copper; superoxide dismutase; alleles; enzyme activity; molecular conformation; heterozygosity; nervous system diseases; histopathology; neurons; photoreceptors; compound eyes; necrosis; disease models
Abstract:
... Mutations in Cu/Zn superoxide dismutase (SOD), a hallmark of familial lateral sclerosis (FALS) in humans, are shown here to confer striking neuropathology in Drosophila. Heterozygotes with one wild-type and one deleted SOD allele retain the expected 50% of normal activity for this dimeric enzyme. However, heterozygotes with one wild-type and one missense SOD allele show lesser SOD activities, rang ...
DOI:
10.1073/pnas.92.19.8574