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You searched for: Journal Proceedings of the National Academy of Sciences of the United States of America Remove constraint Journal: Proceedings of the National Academy of Sciences of the United States of America Publication Year 1994 Remove constraint Publication Year: 1994 Source 1994 v.91 no.10 Remove constraint Source: 1994 v.91 no.10 Subject Saccharomycetales Remove constraint Subject: Saccharomycetales
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1. Identification of Drosophila cytoskeletal proteins by induction of abnormal cell shape in fission yeast

Author:
Edwards, K.A.; Montague, R.A.; Shepard, S.; Edgar, B.A.; Erikson, R.L.; Kiehart, D.P.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1994 v.91 no.10 pp. 4589-4593
ISSN:
0027-8424
Subject:
genetic transformation; Saccharomycetales; phenotype; complementary DNA; animal proteins; cytoskeleton; binding proteins; actin; fungal anatomy; Drosophila melanogaster
Abstract:
... To clone metazoan genes encoding regulators of cell shape, we have developed a functional assay for proteins that affect the morphology of a simple organism, the fission yeast Schizosaccharomyces pombe. A Drosophila melanogaster cDNA library was constructed in an inducible expression vector and transformed into S. pombe. When expression of the Drosophila sequences was induced, aberrant cell shapes ...
DOI:
10.1073/pnas.91.10.4589
PubMed:
8183953
PubMed Central:
PMC43831

2. Top-down construction of an ordered Schizosaccharomyces pombe cosmid library

Author:
Grothues, D.; Cantor, C.R.; Smith, C.L.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1994 v.91 no.10 pp. 4461-4465
ISSN:
0027-8424
Subject:
Saccharomycetales; DNA libraries; restriction mapping; DNA probes; clones; nucleic acid hybridization
Abstract:
... A very rapid and efficient method for sorting and ordering large number's of clones is presented. This top-down mapping approach divides the entire ordering problem into many smaller tasks and analyzes in parallel a gridded membrane array of clones by hybridization with probe pools. The strategy was tested on a 15-fold-coverage Schizosaccharomyces pombe cosmid library. About 1600 clones were assig ...
DOI:
10.1073/pnas.91.10.4461
PubMed:
8183932
PubMed Central:
PMC43805