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You searched for: Journal Proceedings of the National Academy of Sciences of the United States of America Remove constraint Journal: Proceedings of the National Academy of Sciences of the United States of America Publication Year 1994 Remove constraint Publication Year: 1994 Subject structural genes Remove constraint Subject: structural genes
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121. Extension of Autographa californica nuclear polyhedrosis virus host range by interspecific replacement of a short DNA sequence in the p143 helicase gene

Author:
Croizier, G.; Croizier, L.; Argaud, O.; Poudevigne, D.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1994 v.91 no.1 pp. 48-52
ISSN:
0027-8424
Subject:
host range; structural genes; enzymes; Baculoviridae; amino acid sequences; homologous recombination; Autographa californica; Spodoptera frugiperda; pathogenicity; nucleotide sequences; DNA-binding proteins; Bombyx mori; larvae; strains
Abstract:
... Recombinant baculoviruses obtained by coinfection of insect cells with Autographa californica and Bombyx mori nuclear polyhedrosis viruses (AcNPV and BmNPV, respectively) possess a wider in vitro host range than either parent virus. To localize the DNA sequences responsible for this species specificity, we used a two-step method of production and selection of recombinant viruses with altered speci ...
DOI:
10.1073/pnas.91.1.48
PubMed:
8278405
PubMed Central:
PMC42883

122. The frequency of gene targeting in yeast depends on the number of target copies

Author:
Wilson, J.H.; Leung, W.Y.; Bosco, G.; Dieu, D.; Haber, J.E.
Source:
Proceedings of the National Academy of Sciences of the United States of America 1994 v.91 no.1 pp. 177-181
ISSN:
0027-8424
Subject:
genetic transformation; structural genes; Saccharomyces cerevisiae; homologous recombination; gene dosage
Abstract:
... We have compared the efficiency of transformation by linear DNA fragments in yeast strains carrying different numbers of homologous targets for recombination. In strains carrying dispersed copies of a target and in strains carrying tandem arrays, the frequency of transformation is proportional to the number of targets. This result is in contrast to previous studies of transformation in mammalian c ...
DOI:
10.1073/pnas.91.1.177
PubMed:
8278360
PubMed Central:
PMC42909