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You searched for: Journal name Process biochemistry Remove constraint Journal name: Process biochemistry Publication year rev 7979-2021 Remove constraint Publication year rev: 7979-2021 Source 2021 v.102 Remove constraint Source: 2021 v.102
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1. Antidiabetic potential and metabolite profiling of biotransformed polyherbal extract using Wickerhamomyces anomalus strain(MTCC-4133)

Author:
Praveen Kumar Gaur; Sadish Kumar S.; Dinesh Puri
Source:
Process biochemistry 2021 v.102 pp. 199-206
ISSN:
1359-5113
Subject:
Gymnema sylvestre; Mangifera indica; Momordica charantia; Syzygium cumini; Wickerhamomyces anomalus; apigenin; chromatography; diabetes; ethanol; fermentation; fruits; glycemic effect; hesperidin; histology; isorhamnetin; kaempferol; leaves; luteolin; metabolites; quercetin; seeds; therapeutics; toxicity
Abstract:
... Biochemical transformation is a green and efficient strategy that can be of help in case of potentiation of herbal treatments. Diabetes requires daily therapeutic interventions and the use of herbals can be safe and effective alternative however higher doses of herbs can cause much inconvenience. The potentiation of polyherbal combinations to reduce the dosage can be an attractive approach.The pre ...
DOI:
10.1016/j.procbio.2020.12.020

2. Baicalein mediates anticancer effect on cholangiocarcinoma through co-targeting the AKT/NF-κB and STAT3 signaling pathway

Author:
Peng Han; Jin Shang; Dan-Ling Chen; Si-Yang Li; Rui Fan; Rui-Han Li; Hui-Qi Li; Shi-Yang Zhang; Dong-Yan Shen
Source:
Process biochemistry 2021 v.102 pp. 304-314
ISSN:
1359-5113
Subject:
Scutellaria baicalensis; antineoplastic activity; antineoplastic agents; apoptosis; cell cycle checkpoints; flavonoids; glucose 6-phosphate; glycolysis; hexokinase; models; neoplasms; phosphorylation; viability; xenotransplantation
Abstract:
... Baicalein, the primary flavonoid derived from the roots of Scutellaria baicalensis Georgi, is known to exhibit anticancer effects against different types of cancer. However, the anticancer activity and underlying mechanisms of baicalein on cholangiocarcinoma (CCA) have not been elucidated. Here, our results showed that baicalein inhibited the viability and proliferation of CCA cells in a dose and ...
DOI:
10.1016/j.procbio.2021.01.017

3. Biochemical characterization of a β-N-acetylhexosaminidase from Catenibacterium mitsuokai suitable for the synthesis of lacto-N-triose II

Author:
Yihao Liu; Junwen Ma; Ran Shi; Ting Li; Qiaojuan Yan; Zhengqiang Jiang; Shaoqing Yang
Source:
Process biochemistry 2021 v.102 pp. 360-368
ISSN:
1359-5113
Subject:
Bifidobacterium; Catenibacterium mitsuokai; Escherichia coli; Lactobacillus; breast milk; genes; glycosylation; lactose; pH; temperature
Abstract:
... β-N-acetylhexosaminidases have gained much attention for synthesis of functional oligosaccharides, but the relatively low conversion ratio limited their industrial applications. A β-N-acetylhexosaminidase gene (CmHex187) from Catenibacterium mitsuokai was successfully expressed in Escherichia coli. The recombinant enzyme (CmHex187) was purified, biochemically characterized and subjected to the syn ...
DOI:
10.1016/j.procbio.2021.01.013

4. Combined biotransformation processes affect the antioxidant, antidiabetic and protease inhibitory properties of lentils

Author:
Anna Luisa Ferro Casarin; Gabriela Boscariol Rasera; Ruann Janser Soares de Castro
Source:
Process biochemistry 2021 v.102 pp. 250-260
ISSN:
1359-5113
Subject:
alpha-amylase; antioxidants; biotransformation; enzymatic hydrolysis; ferulic acid; germination; lentils; pancreatin; pepsin; proteinase inhibitors; rutin; trypsin; vanillic acid
Abstract:
... This work aimed to evaluate the effects of germination, enzymatic hydrolysis and their combined association on the antioxidant and antidiabetic properties and content of digestive protease inhibitor compounds of lentils. Results showed that the biotransformation of lentils positively impacted their biological activity. For antioxidant properties, the enzymatic hydrolysis of non-germinated lentils ...
DOI:
10.1016/j.procbio.2021.01.011

5. Comparison of different fermentation processes for the vitamin K2 (Menaquinone-7) production by a novel Bacillus velezensis ND strain

Author:
Changle Zhao; Yinping Wan; Guixiang Tang; Qin Jin; Huili Zhang; Zhinan Xu
Source:
Process biochemistry 2021 v.102 pp. 33-41
ISSN:
1359-5113
Subject:
Bacillus velezensis; biosynthesis; culture media; liquid state fermentation; menaquinones; solid state fermentation; soy flour; synthetic genes
Abstract:
... In this study, a novel MK-7 producing bacterial strain, Bacillus velezensis ND, was isolated and characterized. Multiple low-cost substrates were used in Bacillus velezensis ND culture medium to reduce the MK-7 production cost, MK-7 concentration of 43.8 ± 2.8 mg/L was obtained using raw soybean flour by liquid-state fermentation (LSF). To improve the MK-7 production, LSF, biofilm-based fermentati ...
DOI:
10.1016/j.procbio.2020.11.029

6. Covalent binding and in-situ immobilization of lipases on a flexible nanoporous material

Author:
Saba Ghasemi; Maryam Yousefi; Ahmad Nikseresht; Hoda Omidi
Source:
Process biochemistry 2021 v.102 pp. 92-101
ISSN:
1359-5113
Subject:
Humicola insolens; ambient temperature; carboxylic ester hydrolases; nanopores; pH; thermal stability; ultrasonic treatment
Abstract:
... In this study, the flexible nanoporous MIL-53(Fe) (MIL = Materials of Institute Lavoisier) was used as an efficient support for in-situ and covalent binding immobilization of Humicola insolens lipase (HIL) and Rhizomucour miehei lipase (RML). In the covalent attachment procedure, the support synthesized under ultrasound irradiation was functionalized by N,N-dicyclohexylcarbodiimide and then attach ...
DOI:
10.1016/j.procbio.2020.12.013

7. Development of environmentally friendly lipase-catalysed kinetic resolution of (R,S)-1-phenylethyl acetate using aqueous natural deep eutectic solvents

Author:
Manuela Panić; Mia Radović; Izabela Maros; Ana Jurinjak Tušek; Marina Cvjetko Bubalo; Ivana Radojčić Redovniković
Source:
Process biochemistry 2021 v.102 pp. 1-9
ISSN:
1359-5113
Subject:
batch systems; biocatalysts; bioenergy; carboxylic ester hydrolases; choline; ethyl acetate; liquid-liquid extraction; silica gel; solvents
Abstract:
... Over the past decade, natural deep eutectic solvents (NADES) have become promising green solvent from both environmental and technological perspectives. The number of structural combinations encompassed by NADES is tremendous; thus, NADES with unique physicochemical properties can be designed for a particular purpose, such as for efficient biocatalytic processes. The aim of this work was to prepar ...
DOI:
10.1016/j.procbio.2020.12.001

8. Dye wastewater treatment and hydrogen production in microbial electrolysis cells using MoS2-graphene oxide cathode: Effects of dye concentration, co-substrate and buffer solution

Author:
Yanping Hou; Lingli Tu; Shanming Qin; Zebin Yu; Yimin Yan; You Xu; Hainong Song; Hongfei Lin; Yongli Chen; Shuangfei Wang
Source:
Process biochemistry 2021 v.102 pp. 51-58
ISSN:
1359-5113
Subject:
alizarin; azo dyes; cathodes; chemical oxygen demand; clean energy; decolorization; electrochemistry; electrolysis; foams; glucose; hydrogen production; microbial electrolysis cells; microbial growth; nickel; wastewater treatment
Abstract:
... A single-chamber microbial electrolysis cell (MEC) constructed with MoS₂-GO nickel foam (NF) cathode was established for alizarin yellow R (AYR) decolorization and hydrogen production, and influences of AYR initial concentration, co-substrate and buffer on MECs performances were determined. The cathode was obtained by in-situ growing MoS₂-GO on NF. Physico-chemical and electrochemical characteriza ...
DOI:
10.1016/j.procbio.2020.12.008

9. Effect of ethanol or lactic acid on volatile fatty acid profile and microbial community in short-term sequentially transfers by ruminal fermented with wheat straw in vitro

Author:
Miao Lin; Limei Feng; Zhiqiang Cheng; Kuopeng Wang
Source:
Process biochemistry 2021 v.102 pp. 369-375
ISSN:
1359-5113
Subject:
Clostridium; Megasphaera; Ruminococcus; Wallemia; coculture; community structure; ethanol; fatty acid composition; fermentation; fungal communities; hexanoic acid; lactic acid; pH; valeric acid; volatile fatty acids; wastes; wheat straw
Abstract:
... At present, anaerobic fermentation with waste has become a new utilization direction for the production of VFA, and it has advantages for chemical platforms in replacing non-renewable resources that use petrochemicals as raw materials. In this experiment, the pH value, gas production, VFA production, bacterial and fungal community structure of rumen fermented wheat straw with ethanol or lactic aci ...
DOI:
10.1016/j.procbio.2020.12.018

10. Evaluation of the effect of anolyte recirculation and anolyte pH on the performance of a microbial fuel cell employing ceramic separator

Author:
Somdipta Bagchi; Manaswini Behera
Source:
Process biochemistry 2021 v.102 pp. 207-212
ISSN:
1359-5113
Subject:
anaerobes; anodes; biochemistry; ceramics; energy; microbial fuel cells; pH; power generation; wastewater; wastewater treatment
Abstract:
... Microbial fuel cell (MFC) provides a one-stop solution to wastewater treatment and depletion of existing energy resources. One of the major performance limitations of MFC is pH splitting that results in decreased pH of the anolyte affecting the growth of anaerobes. Hence an attempt was made in this study, to provide a suitable pH-controlled environment for microorganisms by recirculating the waste ...
DOI:
10.1016/j.procbio.2021.01.008

11. Immune enhancement and antioxidant effects of low molecular-weight peptides derived from Nibea japonica muscles on immune-deficient mice induced by cyclophosphamide

Author:
Yu Zeng; Xuyang Hu; Zheyu Yu; Fenglei Wang; Zhuangwei Zhang; Kang He; Hengqun Tian; Fangmiao Yu
Source:
Process biochemistry 2021 v.102 pp. 42-50
ISSN:
1359-5113
Subject:
Nibea; antioxidant activity; antioxidants; concanavalin A; cyclophosphamide; dose response; food industry; functional foods; humoral immunity; immune response; interleukin-2; macrophages; molecular weight; oxidative stress; phagocytosis; secretion
Abstract:
... Marine-derived bioactive peptides are widely studied and used as functional foods. The aim of this study was to investigate the effects of low molecular-weight (Mw) peptides (150 Da < Mw < 1 kDa) from Nibea japonica muscles, referred to as Nibea japonica peptides (NJP), on the immune function of mice immunosuppressed with cyclophosphamide (CTX). The results showed that NJP significantly increased ...
DOI:
10.1016/j.procbio.2020.11.016

12. Integrated transcriptome and in vitro analysis revealed antiproliferative effects on human gastric cancer cells by a benzimidazole-quinoline copper(II) complex

Author:
Wei Song; Pingluo Xu; Shuangcheng Zhi; Saijie Zhu; Yan Guo; Haibo Yang
Source:
Process biochemistry 2021 v.102 pp. 286-295
ISSN:
1359-5113
Subject:
antineoplastic activity; apoptosis; cell movement; copper; death; drug therapy; humans; medicine; mitochondria; oxidative stress; stomach neoplasms; transcriptome; transcriptomics
Abstract:
... Developing novel copper-based complexes as potential chemotherapeutic agents is a great challenge in antitumor medicine. In this study, we aimed to reveal a benzimidazole-quinoline copper-based complex 1 induced antitumor activity in human gastric cancer. Complex 1 induced oxidative stress and increase the level of intracellular ROS expression. Further, complex 1 induced proliferation inhibition, ...
DOI:
10.1016/j.procbio.2021.01.009

13. Metabolomic analysis reveals Kluyveromyces marxianus’s stress responses during high-temperature ethanol fermentation

Author:
Pengsong Li; Xianwei Tan; Xiaofen Fu; Yan Dang; Shizhong Li
Source:
Process biochemistry 2021 v.102 pp. 386-392
ISSN:
1359-5113
Subject:
Kluyveromyces marxianus; bioethanol; biosynthesis; ethanol fermentation; ethanol production; heat tolerance; metabolome; metabolomics; oleic acid; palmitic acid; palmitoleic acid; pyridoxine; riboflavin; stress tolerance; temperature; yeasts
Abstract:
... The thermotolerant yeast Kluyveromyces marxianus is a promising bioethanol producer, but its fermentation arrested earlier at high temperatures. Untargeted metabolomic analysis was used to explore K. marxianus’s stress responses during high-temperature fermentation. Fermentation experiments were conducted at 45 °C, with a group conducted at 30 °C as control. Samples were collected from both groups ...
DOI:
10.1016/j.procbio.2021.01.024

14. Microbial production of medium-chain length polyhydroxyalkanoates

Author:
Juliana B. Silva; João R. Pereira; Bruno C. Marreiros; Maria A.M. Reis; Filomena Freitas
Source:
Process biochemistry 2021 v.102 pp. 393-407
ISSN:
1359-5113
Subject:
Pseudomonas; biodegradability; carbon; crystal structure; drugs; economic sustainability; energy; feedstocks; glass transition; medicine; polyhydroxyalkanoates
Abstract:
... Medium-chain-length poly(hydroxyalkanoates), mcl-PHAs, are naturally produced polymers characterized by material properties that include low crystallinity and low melting and glass transition temperatures, which render them suitable for diverse applications. These biodegradable and biocompatible biopolymers are accumulated as intracellular energy and carbon reserves by a wide range of bacteria, ma ...
DOI:
10.1016/j.procbio.2021.01.020

15. Positive effect of glycerol on the stability of immobilized enzymes: Is it a universal fact?

Author:
Sabrina Ait Braham; El Hocine Siar; Sara Arana-Peña; Hossein Bavandi; Diego Carballares; Roberto Morellon-Sterling; Diandra de Andrades; Jakub F. Kornecki; Roberto Fernandez-Lafuente
Source:
Process biochemistry 2021 v.102 pp. 108-121
ISSN:
1359-5113
Subject:
carboxylic ester hydrolases; catalase; enzyme stability; ficain; glutaraldehyde; glycerol; glycosidases; immobilized enzymes; laccase; pH; penicillin amidase; stabilizers
Abstract:
... In this paper, the effect of glycerol at different concentrations and pH values on the stabilities of 13 immobilized enzymes has been analyzed. Among the enzymes, we have selected 5 lipases (immobilized on octyl or amino-glutaraldehyde supports), 3 proteases (immobilized on glyoxyl or amino-glutaraldehyde supports), 2 glycosidases, 1 laccase, 1 catalase (immobilized on glutaraldehyde matrix) and o ...
DOI:
10.1016/j.procbio.2020.12.015

16. Precise engineering of iron oxide nanoparticle-encapsulated protein hydrogel: Implications for cardiac toxicity and ultrasound contrast agents

Author:
Qian Zhang; Aili Fan; Jiali Fu; Qian Zhao
Source:
Process biochemistry 2021 v.102 pp. 296-303
ISSN:
1359-5113
Subject:
acoustics; acridine orange; apoptosis; cardiomyocytes; cardiotoxicity; cytotoxicity; doxorubicin; ethidium; humans; hydrogels; membrane potential; metabolism; mitochondrial membrane; nanocomposites; sericin; silk; ultrasonics
Abstract:
... In the present study, we developed and assessed novel injectable hydrogel matrices combined with nanoparticles and secretome biomolecules to reduce Doxorubicin (DOX)- induced cytotoxicity in human stem cell-derived cardiomyocytes. A Fe₂O₃ nanoparticle-loaded biocompatible silk sericin (MSS) nanocomposite form was fabricated and used as an injectable carrier for secretome for in vivo cardiomyocyte ...
DOI:
10.1016/j.procbio.2021.01.021

17. Preparation of di- and tri- galacturonic acid by coupling hydrothermal pretreatment and enzymatic hydrolysis

Author:
Ting Wang; Meiyun Jiang; Shuo Tang; Chenhuan Lai; Caoxing Huang; Yimin Fan; Qiang Yong
Source:
Process biochemistry 2021 v.102 pp. 180-185
ISSN:
1359-5113
Subject:
biorefining; enzymatic hydrolysis; esterification; galacturonic acid; molecular weight; pectins; polygalacturonase; prebiotics; viscosity
Abstract:
... Di-galacturonic acid (Di-GalA) and tri-galacturonic acid (tri-GalA) are emerging as new prebiotics. A novel biorefining strategy was established to prepare di-GalA and tri-GalA (oligogalacturonic acids) from commercial pectin, which utilized a two-stage process consisting of a hydrothermal pretreatment of low-esterification pectin (LP) followed by enzymatic hydrolysis with endo-polygalacturonase. ...
DOI:
10.1016/j.procbio.2021.01.002

18. Process development of recombinant Aspergillus flavus urate oxidase production in Pichia pastoris intracellularly and its characterization as a potential biosimilar

Author:
Yapeng Wang; Jianguang Lu; Zongqing Huang; Mengxin Qian; Qingbin Zhang; Jun Feng
Source:
Process biochemistry 2021 v.102 pp. 376-385
ISSN:
1359-5113
Subject:
Aspergillus flavus; Komagataella pastoris; allantoin; ammonium sulfate; anion exchange chromatography; bioprocessing; cations; culture media; neoplasms; oxidoreductases; polyethyleneimine; response surface methodology; therapeutics; uric acid
Abstract:
... Recombinant A. flavus Urate Oxidase (rUOX), which catalyzes uric acid to allantoin, is highly effective in treating tumor lysis syndrome (TLS). However, its production process is quite complicated, and its use as a therapy is often prohibitive because of the high cost. In this work, we demonstrate, for the first time, a new bioprocess for the production of rUOX expressed in P. pastoris GS115 intra ...
DOI:
10.1016/j.procbio.2021.01.026

19. Rational protein engineering of α-L-arabinofuranosidase from Aspergillus niger for improved catalytic hydrolysis efficiency on kenaf hemicellulose

Author:
Nardiah Rizwana Jaafar; Siti Norbaidurah Ayob; Noor Hidayah Abd Rahman; Farah Diba Abu Bakar; Abdul Munir Abdul Murad; Rosli Md Illias
Source:
Process biochemistry 2021 v.102 pp. 349-359
ISSN:
1359-5113
Subject:
Aspergillus niger; Hibiscus cannabinus; active sites; biomass; catalytic activity; enzymatic hydrolysis; fossils; hemicellulose; hydrolysis; hydrophobicity; kenaf; lignocellulose
Abstract:
... Lignocellulosic biomass utilisation as an alternative to fossil resources was hampered due to its recalcitrant to enzymatic hydrolysis. Herein, rational protein engineering strategy has been carried out on Aspergillus niger α-L-arabinofuranosidase (AnabfA) substrate binding pathway to improve its catalytic efficiency towards pre-treated kenaf (Hibiscus cannabinus) hemicellulose hydrolysis. A total ...
DOI:
10.1016/j.procbio.2020.12.012

20. The synthesis of l-glycyl-l-tyrosine derivatives using organic-solvent stable PST-01 protease from Pseudomonas aeruginosa PST-01

Author:
Takuya Matsumoto; Ruri Kitayama; Ryosuke Yamada; Hiroyasu Ogino
Source:
Process biochemistry 2021 v.102 pp. 186-189
ISSN:
1359-5113
Subject:
Pseudomonas aeruginosa; dimethyl sulfoxide; dipeptides; humans; proteinases; solubility; tyrosine
Abstract:
... The aqueous solubility of some amino acids is very low which can be improved by dipeptides in case of these molecules. Although tyrosine is one of those amino acids that have a low aqueous solubility, dipeptides on the other side, such as l-glycyl-l-tyrosine (Gly-Tyr), have high aqueous solubility. Gly-Tyr is rapidly degraded into l-glycine and l-tyrosine in the human body and useful as a transfus ...
DOI:
10.1016/j.procbio.2021.01.005