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- Liang, Youxiang; Jiao, Song; Wang, Miaomiao; Yu, Huimin; Shen, Zhongyao
- Process biochemistry 2019 v.79 pp. 49-56
- Agrobacterium radiobacter; Escherichia coli; Rhodococcus ruber; biocatalysts; enantiomers; epichlorohydrins; epoxide hydrolase; epoxides; glycols; pH; proteins; thermal stability
- ... Epoxide hydrolases (EHs) are attractive enzymes for producing enantiopure epoxides and diols, but do not display enough stability when lysates or Escherichia coli whole cells are used as biocatalysts. In this work, an organic-solvent tolerant strain Rhodococcus ruber THdAdN was utilized to overexpress an epoxide hydrolase from Agrobacterium radiobacter (ArEH), using E. coli BL21(DE3) as a control. ...
- Aguirre-Pranzoni, Celeste; Tosso, Rodrigo D.; Bisogno, Fabricio R.; Kurina-Sanz, Marcela; Orden, Alejandro A.
- Process biochemistry 2019 v.79 pp. 114-117
- Rhodotorula rubra; alkynes; ambient temperature; beta adrenergic receptors; bioactive properties; biocatalysts; copper; copper sulfate; cycloaddition reactions; enantiomers; ketones; sodium azide; stereoselectivity; triazoles
- ... Chemoenzymatic strategies for the preparation of enantiopure β-hydroxytriazoles were designed. These and other related compounds are particularly relevant because of their antitubercular bioactivities and as β-adrenergic receptor blockers. The ability of Rhodotorula mucilaginosa LSL to stereoselectively reduce prochiral ketones coupled to copper(I)-catalyzed azide-alkyne cycloaddition was exploite ...