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1. Enhanced production of lignans and neolignans in chitosan-treated flax (Linum usitatissimum L.) cell cultures

Author:
Ahmad, Waqar; Zahir, Adnan; Nadeem, Muhammad; Garros, Laurine; Drouet, Samantha; Renouard, Sullivan; Doussot, Joël; Giglioli-Guivarc’h, Nathalie; Hano, Christophe; Abbasi, Bilal Haider
Source:
Process biochemistry 2019 v.79 pp. 155-165
ISSN:
1359-5113
Subject:
Linum usitatissimum; antioxidant activity; biosynthesis; cell culture; chitosan; diodes; enzyme activity; flax; gene expression; genes; glucosides; high performance liquid chromatography; lignans; linseed; medicinal properties; metabolites; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; transgenic plants
Abstract:
... Linum usitatissimum is a source of pharmacologically active lignans and neolignans. An effective protocol has been established for the enhanced biosynthesis of lignans and neolignans in cell cultures of Linum usitatissimum by using chitosan addition. Gene expression analysis of monolignols (PAL, CCR and CAD), lignans (DIR, PLR and UGT) and neolignans (PCBER) biosynthetic genes by RT-qPCR as well a ...
DOI:
10.1016/j.procbio.2018.12.025