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- Lim, Lihui; Senba, Hironori; Kimura, Yukihiro; Yokota, Satoko; Doi, Mikiharu; Yoshida, Ken-Ichi; Takenaka, Shinji
- Process biochemistry 2019 v.79 pp. 74-80
- thermal stability; Komagataella pastoris; decolorization; polyacrylamide gel electrophoresis; industry; molecular weight; Aspergillus glaucus; sequence analysis; meat; mutagenesis; tuna; water content; hydrolysis; aspartic proteinases; detergents; meat processing; glycosylation; hemoglobin; pH; mutants; heme proteins; water activity
- ... Aspergillus glaucus MA0196 produces a highly glycosylated aspartic protease (PepA_MA0196) that shows hydrolytic and decolorization activities toward hemoglobin. Sequence analysis of PepA_MA0196 indicated two potential N-glycosylation consensus sites, at Asn131 and Asn275. To elucidate the role of N-glycosylation in the biochemical properties of PepA_MA0196 and the resulting effects, recombinant Pe ...