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1. Influences of N-linked glycosylation on the biochemical properties of aspartic protease from Aspergillus glaucus MA0196

Author:
Lim, Lihui; Senba, Hironori; Kimura, Yukihiro; Yokota, Satoko; Doi, Mikiharu; Yoshida, Ken-Ichi; Takenaka, Shinji
Source:
Process biochemistry 2019 v.79 pp. 74-80
ISSN:
1359-5113
Subject:
thermal stability; Komagataella pastoris; decolorization; polyacrylamide gel electrophoresis; industry; molecular weight; Aspergillus glaucus; sequence analysis; meat; mutagenesis; tuna; water content; hydrolysis; aspartic proteinases; detergents; meat processing; glycosylation; hemoglobin; pH; mutants; heme proteins; water activity
Abstract:
... Aspergillus glaucus MA0196 produces a highly glycosylated aspartic protease (PepA_MA0196) that shows hydrolytic and decolorization activities toward hemoglobin. Sequence analysis of PepA_MA0196 indicated two potential N-glycosylation consensus sites, at Asn131 and Asn275. To elucidate the role of N-glycosylation in the biochemical properties of PepA_MA0196 and the resulting effects, recombinant Pe ...
DOI:
10.1016/j.procbio.2018.12.017