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1. Overexpression of epoxide hydrolase in Rhodococcus ruber with high robustness for the synthesis of chiral epichlorohydrin

Author:
Liang, Youxiang; Jiao, Song; Wang, Miaomiao; Yu, Huimin; Shen, Zhongyao
Source:
Process biochemistry 2019 v.79 pp. 49-56
ISSN:
1359-5113
Subject:
Agrobacterium radiobacter; Escherichia coli; Rhodococcus ruber; biocatalysts; enantiomers; epichlorohydrins; epoxide hydrolase; epoxides; glycols; pH; proteins; thermal stability
Abstract:
... Epoxide hydrolases (EHs) are attractive enzymes for producing enantiopure epoxides and diols, but do not display enough stability when lysates or Escherichia coli whole cells are used as biocatalysts. In this work, an organic-solvent tolerant strain Rhodococcus ruber THdAdN was utilized to overexpress an epoxide hydrolase from Agrobacterium radiobacter (ArEH), using E. coli BL21(DE3) as a control. ...
DOI:
10.1016/j.procbio.2018.12.023