Jump to Main Content
- Liang, Youxiang; Jiao, Song; Wang, Miaomiao; Yu, Huimin; Shen, Zhongyao
- Process biochemistry 2019 v.79 pp. 49-56
- Agrobacterium radiobacter; Escherichia coli; Rhodococcus ruber; biocatalysts; enantiomers; epichlorohydrins; epoxide hydrolase; epoxides; glycols; pH; proteins; thermal stability
- ... Epoxide hydrolases (EHs) are attractive enzymes for producing enantiopure epoxides and diols, but do not display enough stability when lysates or Escherichia coli whole cells are used as biocatalysts. In this work, an organic-solvent tolerant strain Rhodococcus ruber THdAdN was utilized to overexpress an epoxide hydrolase from Agrobacterium radiobacter (ArEH), using E. coli BL21(DE3) as a control. ...
- Yu, Bin; Cheng, Hao; Zhuang, Wei; Zhu, ChenJie; Wu, Jinglan; Niu, Huanqing; Liu, Dong; Chen, Yong; Ying, Hanjie
- Process biochemistry 2019 v.79 pp. 40-48
- Fourier transform infrared spectroscopy; X-ray photoelectron spectroscopy; alkalinity; amines; biodegradability; cellulose; glucose; glutaraldehyde; immobilized enzymes; moieties; pH; peroxidase; proteins; scanning electron microscopy; thermogravimetry
- ... Bacterial cellulose (BC) is a biodegradable material with many excellent properties for enzyme immobilization. However, hydroxyl groups of low reactivity in glucose units cannot directly react with the amines of enzymatic proteins. In this work, amino-functionalized bacterial cellulose was firstly used as carrier to immobilize horseradish peroxidase (HRP) via glutaraldehyde coupling. SEM, FT-IR, X ...