Journal: Process biochemistry / Publication Year: 2019 / Source: 2019 v.79 / Subject: pH and proteins

Main content area

Author:: Liang, Youxiang; Jiao, Song; Wang, Miaomiao; Yu, Huimin; Shen, Zhongyao
Source:: Process biochemistry 2019 v.79 pp. 49-56
ISSN:: 1359-5113
Subject:: Agrobacterium radiobacter; Escherichia coli; Rhodococcus ruber; biocatalysts; enantiomers; epichlorohydrins; epoxide hydrolase; epoxides; glycols; pH; proteins; thermal stability
Abstract:: ... Epoxide hydrolases (EHs) are attractive enzymes for producing enantiopure epoxides and diols, but do not display enough stability when lysates or Escherichia coli whole cells are used as biocatalysts. In this work, an organic-solvent tolerant strain Rhodococcus ruber THdAdN was utilized to overexpress an epoxide hydrolase from Agrobacterium radiobacter (ArEH), using E. coli BL21(DE3) as a control. ...
DOI:: 10.1016/j.procbio.2018.12.023
: https://dx.doi.org/10.1016/j.procbio.2018.12.023

Author:: Yu, Bin; Cheng, Hao; Zhuang, Wei; Zhu, ChenJie; Wu, Jinglan; Niu, Huanqing; Liu, Dong; Chen, Yong; Ying, Hanjie
Source:: Process biochemistry 2019 v.79 pp. 40-48
ISSN:: 1359-5113
Subject:: Fourier transform infrared spectroscopy; X-ray photoelectron spectroscopy; alkalinity; amines; biodegradability; cellulose; glucose; glutaraldehyde; immobilized enzymes; moieties; pH; peroxidase; proteins; scanning electron microscopy; thermogravimetry
Abstract:: ... Bacterial cellulose (BC) is a biodegradable material with many excellent properties for enzyme immobilization. However, hydroxyl groups of low reactivity in glucose units cannot directly react with the amines of enzymatic proteins. In this work, amino-functionalized bacterial cellulose was firstly used as carrier to immobilize horseradish peroxidase (HRP) via glutaraldehyde coupling. SEM, FT-IR, X ...
DOI:: 10.1016/j.procbio.2018.12.024
: https://dx.doi.org/10.1016/j.procbio.2018.12.024