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You searched for: Journal Process biochemistry Remove constraint Journal: Process biochemistry Publication Year 2019 Remove constraint Publication Year: 2019 Subject biocatalysts Remove constraint Subject: biocatalysts
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1. A facile route to preparation of immobilized cellulase on polyurea microspheres for improving catalytic activity and stability

Author:
Sui, Ying; Cui, Yu; Xia, Guangming; Peng, Xiujing; Yuan, Guangzheng; Sun, Guoxin
Source:
Process biochemistry 2019 v.87 pp. 73-82
ISSN:
1359-5113
Subject:
Fourier transform infrared spectroscopy; ambient temperature; atomic force microscopy; biocatalysts; catalytic activity; cellulose; chemical industry; dimethyl sulfoxide; endo-1,4-beta-glucanase; enzyme activity; hydrolysis; mass transfer; microparticles; mixing; pH; porous media; scanning electron microscopy; sustainable technology; thermal stability; thermogravimetry
Abstract:
... The input-output ratio of the immobilization process is crucial to determining whether the immobilized cellulase can truly participate in industrial production. This study reports a simple, efficient and environmentally friendly method for covalent immobilization of cellulase on uniform, monodisperse polyurea microspheres. The reported method offers several benefits including (i) polyurea microsph ...
DOI:
10.1016/j.procbio.2019.09.002

2. Modulation of Lecitase properties via immobilization on differently activated Immobead-350: Stabilization and inversion of enantiospecificity

Author:
Pinheiro, Maísa P.; Monteiro, Rodolpho R.C.; Silva, Francisco F.M.; Lemos, Telma L.G.; Fernandez-Lafuente, Roberto; Gonçalves, Luciana R.B.; dos Santos, José C.S.
Source:
Process biochemistry 2019 v.87 pp. 128-137
ISSN:
1359-5113
Subject:
acetates; acetonitrile; biocatalysts; butyrates; catalytic activity; enzymes; epoxides; hydrolysis; pH
Abstract:
... Lecitase Ultra (Leci) was immobilized on Immobead-350 (IB-350) activated with epoxy (EPO), amino-divinylsulfone (DVS) or amino-glutaraldehyde (GLU) groups. Leci-GLU had 27% more activity versus p-nitro-phenyl butyrate (p-NPB) than Leci-EPO, after 3 h of immobilization using 100 mg of enzyme/g of support (immobilization yield was 84.1%, expressed activity versus p-NPB was around 10%), the covalent ...
DOI:
10.1016/j.procbio.2019.08.016

3. Regioselective hydroxylation pathway of tenatoprazole to produce human metabolites by Bacillus megaterium CYP102A1

Author:
Le, Thien-Kim; Cha, Gun-Su; Jang, Hyun-Hee; Nguyen, Thi Huong Ha; Doan, Tiep Thi My; Lee, Young Ju; Park, Ki Deok; Shin, Yumi; Kim, Dong-Hyun; Yun, Chul-Ho
Source:
Process biochemistry 2019 v.87 pp. 95-104
ISSN:
1359-5113
Subject:
Bacillus megaterium; biocatalysts; catalytic activity; drugs; enzymes; esophageal diseases; gastric acid; hydroxylation; hypersecretion; liver; metabolites; mutants; proton pump inhibitors; regioselectivity; stomach ulcers; synthesis
Abstract:
... Tenatoprazole, a proton pump inhibitor drug candidate, is developed as an acid inhibitor to treat gastric acid hypersecretion disorders, such as gastric ulcer and reflux esophagitis. Tenatoprazole is known to be metabolized to three major metabolites—tenatoprazole sulfone, 5’-hydroxylated metabolite, and tenatoprazole sulfide—in human livers mainly by CYP2C19 and CYP3A4. In this study, an enzymati ...
DOI:
10.1016/j.procbio.2019.09.014

4. Improvement of cross-linking and stability on cross-linked enzyme aggregate (CLEA)-xylanase by protein surface engineering

Author:
Abdul Wahab, Mohd Khairul Hakimi; El-Enshasy, Hesham Ali; Bakar, Farah Diba Abu; Murad, Abdul Munir Abdul; Jahim, Jamaliah Md; Illias, Rosli Md
Source:
Process biochemistry 2019 v.86 pp. 40-49
ISSN:
1359-5113
Subject:
Aspergillus fumigatus; biocatalysts; bioenergy industry; catalytic activity; cost effectiveness; crosslinking; engineering; glutaraldehyde; immobilized enzymes; mutants; prebiotics; site-directed mutagenesis; xylanases
Abstract:
... Cross-linked enzyme aggregate (CLEA) is a well-known enzyme immobilization technique that is efficient and cost-effective. In this study, we proposed a combination of the surface modification of an enzyme and CLEA to increase the number of free amino groups. Site-directed mutagenesis was applied to selected residues of xylanase from Aspergillus fumigatus RT-1 and the xylanase was subsequently cros ...
DOI:
10.1016/j.procbio.2019.07.017

5. Tuning dimeric formate dehydrogenases reduction/oxidation activities by immobilization

Author:
Yildirim, Deniz; Alagöz, Dilek; Toprak, Ali; Tükel, Seyhan; Fernandez-Lafuente, Roberto
Source:
Process biochemistry 2019 v.85 pp. 97-105
ISSN:
1359-5113
Subject:
Candida boidinii; adsorption; biocatalysts; calcium alginate; carbon dioxide; carbonates; encapsulation; formate dehydrogenase; formates; formic acid; half life; hydrogen; immobilized enzymes; montmorillonite; oxidation; pH; polyvinyl alcohol; sodium bicarbonate; thermal stability
Abstract:
... Three differently immobilized preparations of an enzyme extract from Candida boidinii containing a NAD+-dependent formate dehydrogenase (CbFDH) were prepared by encapsulation using calcium alginate (CbFDH-Alg) or polyvinyl alcohol (CbFDH-PVA) or by adsorption on montmorillonite K 10 (CbFDH-Mont). All FDH preparations were characterized in terms of both oxidation and reduction activities. For the f ...
DOI:
10.1016/j.procbio.2019.07.001

6. Efficient accumulation of sclerotiorin via overcoming low pH caused by overflow carbon metabolism during cell suspension culture of Penicillium sclerotiorum

Author:
Zheng, Jiawei; Zhang, Xuehong; Xin, Qiao; Pan, Tao; Wang, Zhilong
Source:
Process biochemistry 2019 v.82 pp. 32-39
ISSN:
1359-5113
Subject:
Crabtree effect; Penicillium; absorbance; biocatalysts; biomass; biosynthesis; carbon; carbon metabolism; cell suspension culture; glucose; lactose; liquids; malic acid; mixing; mycelium; pH; phosphates; plankton; secondary metabolites; solid state fermentation
Abstract:
... Sclerotiorin is the secondary metabolite of Penicillium sclerotiorum, which is usually produced by solid state fermentation or liquid culture without stirring (formation of mycelial mat on air-liquid surface). In the present work, it was confirmed that malic acid was produced during cell suspension culture in a liquid medium (mycelia acting as planktonic cells) using resting cells as whole cell bi ...
DOI:
10.1016/j.procbio.2019.05.003

7. High-fold improvement of assorted post-consumer poly(ethylene terephthalate) (PET) packages hydrolysis using Humicola insolens cutinase as a single biocatalyst

Author:
Castro, Aline Machado de; Carniel, Adriano; Stahelin, Diego; Chinelatto Junior, Luiz Silvino; Honorato, Hercilio de Angeli; de Menezes, Sonia Maria Cabral
Source:
Process biochemistry 2019 v.81 pp. 85-91
ISSN:
1359-5113
Subject:
Humicola insolens; biocatalysts; circular economy; cutinase; depolymerization; hydrolysis; plastics; polyethylene terephthalates; recycling
Abstract:
... The dissemination of technologies for poly(ethylene terephthalate) (PET) recycling is of paramount importance in the context of the plastics circular economy. One of the most promising alternatives is to use enzymes as catalysts for PET depolymerization to its monomers, but this route still needs improvement, especially regarding titer and productivity. In the present work, a sequential approach c ...
DOI:
10.1016/j.procbio.2019.03.006

8. t-Butyl 6-cyano-(3R,5R)-dihydroxyhexanoate synthesis via asymmetric reduction by immobilized cells of carbonyl reductase and glucose dehydrogenase co-expression E. coli

Author:
Qiu, Shuai; Wang, Ya-Jun; Yu, Han; Cheng, Feng; Zheng, Yu-Guo
Source:
Process biochemistry 2019 v.80 pp. 43-51
ISSN:
1359-5113
Subject:
Escherichia coli; activated carbon; adsorption; atorvastatin; biocatalysts; coatings; coordination polymers; crosslinking; enzymes; glucose; glutaraldehyde; immobilized cells
Abstract:
... Chiral diol t-butyl 6-cyano-(3R,5R)-dihydroxyhexanoate ((3R,5R)-2) is an important building block of atorvastatin calcium. In our previous works, we constructed a glucose dehydrogenase (GDH) and carbonyl reductase (CR) co-expression strain E. coli BL21(DE3)/pCDFDuet-gdh-cr, which was capable for asymmetrically reducing t-butyl 6-cyano-(5R)-hydroxyl-3-oxolhexanoate ((5R)-1) to (3R,5R)-2. In order t ...
DOI:
10.1016/j.procbio.2019.02.019

9. Overexpression of epoxide hydrolase in Rhodococcus ruber with high robustness for the synthesis of chiral epichlorohydrin

Author:
Liang, Youxiang; Jiao, Song; Wang, Miaomiao; Yu, Huimin; Shen, Zhongyao
Source:
Process biochemistry 2019 v.79 pp. 49-56
ISSN:
1359-5113
Subject:
Agrobacterium radiobacter; Escherichia coli; Rhodococcus ruber; biocatalysts; enantiomers; epichlorohydrins; epoxide hydrolase; epoxides; glycols; pH; proteins; thermal stability
Abstract:
... Epoxide hydrolases (EHs) are attractive enzymes for producing enantiopure epoxides and diols, but do not display enough stability when lysates or Escherichia coli whole cells are used as biocatalysts. In this work, an organic-solvent tolerant strain Rhodococcus ruber THdAdN was utilized to overexpress an epoxide hydrolase from Agrobacterium radiobacter (ArEH), using E. coli BL21(DE3) as a control. ...
DOI:
10.1016/j.procbio.2018.12.023

10. Preparation of chiral β-hydroxytriazoles in one-pot chemoenzymatic bioprocesses catalyzed by Rhodotorula mucilaginosa

Author:
Aguirre-Pranzoni, Celeste; Tosso, Rodrigo D.; Bisogno, Fabricio R.; Kurina-Sanz, Marcela; Orden, Alejandro A.
Source:
Process biochemistry 2019 v.79 pp. 114-117
ISSN:
1359-5113
Subject:
Rhodotorula rubra; alkynes; ambient temperature; beta adrenergic receptors; bioactive properties; biocatalysts; copper; copper sulfate; cycloaddition reactions; enantiomers; ketones; sodium azide; stereoselectivity; triazoles
Abstract:
... Chemoenzymatic strategies for the preparation of enantiopure β-hydroxytriazoles were designed. These and other related compounds are particularly relevant because of their antitubercular bioactivities and as β-adrenergic receptor blockers. The ability of Rhodotorula mucilaginosa LSL to stereoselectively reduce prochiral ketones coupled to copper(I)-catalyzed azide-alkyne cycloaddition was exploite ...
DOI:
10.1016/j.procbio.2018.12.011

11. Strategies for enriching erucic acid from Crambe abyssinica oil by improved Candida antarctica lipase A variants

Author:
Zorn, Katja; Oroz-Guinea, Isabel; Bornscheuer, Uwe T.
Source:
Process biochemistry 2019 v.79 pp. 65-73
ISSN:
1359-5113
Subject:
Crambe abyssinica; Pseudozyma antarctica; biocatalysts; carboxylic ester hydrolases; crambe seed oil; erucic acid; esterification; esters; hydrolysis; industry; mutants; protein engineering; temperature profiles
Abstract:
... Erucic acid (C22:1, Δ13Z) is an interesting building block for the oleochemical industry and is abundantly present in Crambe abyssinica seed oil (∼59% of C22:1). For the enrichment of fatty acids (FA), lipases can be employed and we have found in a previous protein engineering study suitable variants of lipase A from Moesziomyces antarcticus (CAL-A) for the enrichment of C22:1 from Crambe oil deri ...
DOI:
10.1016/j.procbio.2018.12.022

12. Benzyl butyrate esterification mediated by immobilized lipases: Evaluation of batch and fed-batch reactors to overcome lipase-acid deactivation

Author:
de Meneses, Alessandra Cristina; Almeida Sá, Amanda Gomes; Lerin, Lindomar Alberto; Corazza, Marcos Lúcio; de Araújo, Pedro Henrique Hermes; Sayer, Claudia; de Oliveira, Débora
Source:
Process biochemistry 2019 v.78 pp. 50-57
ISSN:
1359-5113
Subject:
Pseudozyma antarctica; benzyl alcohol; biocatalysts; butyric acid; carboxylic ester hydrolases; esterification; immobilized enzymes
Abstract:
... In this work, benzyl butyrate was synthesized for the first time via biotechnological esterification of benzyl alcohol and butyric acid in a solvent-free system (SFS). The synthesis was maximized in batch reactors, considering a low substrates molar ratio of 1:1. Novozym 435, a well-known commercial immobilized lipase (Candida antarctica fraction B - Cal B), was the best biocatalyst in the benzyl ...
DOI:
10.1016/j.procbio.2018.12.029

13. Co-immobilization of dextransucrase and dextranase in epoxy-agarose- tailoring oligosaccharides synthesis

Author:
da Silva, Rhonyele Maciel; Paiva Souza, Priscila Maria; Fernandes, Fabiano A.N.; Gonçalves, Luciana R.B.; Rodrigues, Sueli
Source:
Process biochemistry 2019 v.78 pp. 71-81
ISSN:
1359-5113
Subject:
Chaetomium; Leuconostoc mesenteroides; biocatalysts; catalytic activity; dextran; dextranase; dextransucrase; markets; oligosaccharides; pH; polymerization; temperature
Abstract:
... The best support reported for dextransucrase (DS) and dextranase (DN) immobilization was Eupergit C, but this carrier was discontinued and is no longer available in the market. Thus, epoxy-agarose support is suggested as an alternative to co-immobilize DS from Leuconostoc mesenteroides B-512F and DN from Chaetomium erraticum. The co-immobilization approach improved DS performance, compared to immo ...
DOI:
10.1016/j.procbio.2019.01.009

14. Expression of xylanase on Escherichia coli using a truncated ice nucleation protein of Erwinia ananas (InaA)

Author:
Wee, Mei Yuin Joanne; Abd. Murad, Abdul Munir; Abu Bakar, Farah Diba; Low, Kheng Oon; Md Illias, Rosli
Source:
Process biochemistry 2019 v.78 pp. 25-32
ISSN:
1359-5113
Subject:
Escherichia coli; Pantoea ananatis; Western blotting; biocatalysts; enzyme activity; flow cytometry; ice nucleation; polyacrylamide gel electrophoresis; xylan; xylanases
Abstract:
... Cell surface display involves the anchoring of enzymes on the surface of cells to be used as whole-cell biocatalysts. In this study, an ice nucleation protein (INP) anchor from Erwinia ananas IN-10, InaA, was used to display xylanase. The ability of InaA to be targeted to the outer membrane was compared to cells displaying xylanase using two other INPs, InaK and InaZ. SDS-PAGE and western blot of ...
DOI:
10.1016/j.procbio.2019.01.005

15. Synthesis of diethylhexyl adipate by Candida antarctica lipase-catalyzed esterification

Author:
Kim, Heejin; Kim, Taehoon; Choi, Nakyung; Kim, Byung Hee; Oh, Se-Wook; Kim, In-Hwan
Source:
Process biochemistry 2019 v.78 pp. 58-62
ISSN:
1359-5113
Subject:
Pseudozyma antarctica; adipic acid; biocatalysts; carboxylic ester hydrolases; chemical industry; esterification; plasticizers; poly(vinyl chloride); temperature
Abstract:
... Diethylhexyl adipate (DEHA) has been used for various applications in the chemical industry. In particular, DEHA is used as a plasticizer for polyvinyl chloride and its polymers. In this study, DEHA was successfully synthesized via lipase-catalyzed esterification from adipic acid with ethylhexanol in a solvent-free system. An immobilized lipase from Candida antarctica (Novozym 435) was used as a b ...
DOI:
10.1016/j.procbio.2018.12.030