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1. Characterisation of five alcohol dehydrogenases from Lactobacillus reuteri DSM20016

Author:
Hu, Zhenghong; Jia, Pu; Bai, Yajun; Fan, Tai-ping; Zheng, Xiaohui; Cai, Yujie
Source:
Process biochemistry 2019 v.86 pp. 73-79
ISSN:
1359-5113
Subject:
Escherichia coli; Lactobacillus reuteri; alcohol dehydrogenase; alcohols; aldehydes; catalytic activity; cheese ripening; cheeses; enzyme activity; molecular weight; odors; pH; proteins; temperature
Abstract:
... Alcohol dehydrogenases convert aldehydes causing volatile aromas in cheese into corresponding alcohols. In this study, we cloned and recombinantly produced five novel ADHs from Lactobacillus reuteri DSM20016 in Escherichia coli BL21 (DE3). The resulting proteins have molecular weights between 35 and 39 kDa. The optimum pH for enzyme activity of ADH-1, ADH-2 and ADH-3 was weakly acidic (pH 5, 6 and ...
DOI:
10.1016/j.procbio.2019.08.010

2. Sequential hydrolysis of spent brewer's yeast improved its physico-chemical characteristics and antioxidant properties: A strategy to transform waste into added-value biomolecules

Author:
Marson, Gabriela Vollet; Machado, Mariana Teixeira da Costa; de Castro, Ruann Janser Soares; Hubinger, Miriam Dupas
Source:
Process biochemistry 2019 v.84 pp. 91-102
ISSN:
1359-5113
Subject:
antioxidant activity; autolysis; beers; brewers yeast; byproducts; cell walls; crude protein; environmental impact; enzymatic hydrolysis; hydrolysates; hydrolysis; pH; peptides; proteinases; proteins; raw materials; wastes
Abstract:
... The spent brewer's yeast (SBY) is a promising raw material due to its high content of proteins, but cells must be disrupted to release yeast compounds. Our work aimed to study the rupturing of SBY cell wall comparing conventional methods (autolysis and mechanical rupture) with enzymatic hydrolysis using proteolytic enzymes (Brauzyn®, Alcalase™, Protamex™ and Flavourzyme™). The susceptibility to ru ...
DOI:
10.1016/j.procbio.2019.06.018

3. R-phycoerythrin, R-phycocyanin and ABE production from Gelidium amansii by Clostridium acetobutylicum

Author:
Sukwong, Pailin; Sunwoo, In Yung; Nguyen, Trung Hau; Jeong, Gwi-Taek; Kim, Sung-Koo
Source:
Process biochemistry 2019 v.81 pp. 139-147
ISSN:
1359-5113
Subject:
Clostridium acetobutylicum; Gelidium; acid hydrolysis; cell growth; fermentation; galactose; glucose; macroalgae; pH; polysaccharides; proteins; saccharification; solvents
Abstract:
... The red seaweed, Gelidium amansii, contains proteins such as R-phycoerythrin and R-phycocyanin, as well as polysaccharides. Initial protein extraction from G. amansii yielded 53 μg/g R-phycoerythrin and 56 μg/g R-phycocyanin. After protein extraction, monosaccharides were produced using hyper thermal acid hydrolysis and enzymatic saccharification. Acetone–butanol–ethanol (ABE) was produced by Clos ...
DOI:
10.1016/j.procbio.2019.03.023

4. Overexpression of epoxide hydrolase in Rhodococcus ruber with high robustness for the synthesis of chiral epichlorohydrin

Author:
Liang, Youxiang; Jiao, Song; Wang, Miaomiao; Yu, Huimin; Shen, Zhongyao
Source:
Process biochemistry 2019 v.79 pp. 49-56
ISSN:
1359-5113
Subject:
Agrobacterium radiobacter; Escherichia coli; Rhodococcus ruber; biocatalysts; enantiomers; epichlorohydrins; epoxide hydrolase; epoxides; glycols; pH; proteins; thermal stability
Abstract:
... Epoxide hydrolases (EHs) are attractive enzymes for producing enantiopure epoxides and diols, but do not display enough stability when lysates or Escherichia coli whole cells are used as biocatalysts. In this work, an organic-solvent tolerant strain Rhodococcus ruber THdAdN was utilized to overexpress an epoxide hydrolase from Agrobacterium radiobacter (ArEH), using E. coli BL21(DE3) as a control. ...
DOI:
10.1016/j.procbio.2018.12.023

5. Stability and repeatability improvement of horseradish peroxidase by immobilization on amino-functionalized bacterial cellulose

Author:
Yu, Bin; Cheng, Hao; Zhuang, Wei; Zhu, ChenJie; Wu, Jinglan; Niu, Huanqing; Liu, Dong; Chen, Yong; Ying, Hanjie
Source:
Process biochemistry 2019 v.79 pp. 40-48
ISSN:
1359-5113
Subject:
Fourier transform infrared spectroscopy; X-ray photoelectron spectroscopy; alkalinity; amines; biodegradability; cellulose; glucose; glutaraldehyde; immobilized enzymes; moieties; pH; peroxidase; proteins; scanning electron microscopy; thermogravimetry
Abstract:
... Bacterial cellulose (BC) is a biodegradable material with many excellent properties for enzyme immobilization. However, hydroxyl groups of low reactivity in glucose units cannot directly react with the amines of enzymatic proteins. In this work, amino-functionalized bacterial cellulose was firstly used as carrier to immobilize horseradish peroxidase (HRP) via glutaraldehyde coupling. SEM, FT-IR, X ...
DOI:
10.1016/j.procbio.2018.12.024