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You searched for: Journal Process biochemistry Remove constraint Journal: Process biochemistry Publication Year 2018 Remove constraint Publication Year: 2018 Source 2018 v.73 Remove constraint Source: 2018 v.73
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1. A novel ACE inhibitory peptide derived from alkaline hydrolysis of ostrich (Struthio camelus) egg white ovalbumin

Author:
Khueychai, Siriporn; Jangpromma, Nisachon; Choowongkomon, Kiattawee; Joompang, Anupong; Daduang, Sakda; Vesaratchavest, Mongkol; Payoungkiattikun, Wisarut; Tachibana, Shinjiro; Klaynongsruang, Sompong
Source:
Process biochemistry 2018 v.73 pp. 235-245
ISSN:
1359-5113
Subject:
Struthio camelus; absorption; alkaline hydrolysis; anion exchange chromatography; cytotoxicity; digestion; egg albumen; erythrocytes; fibroblasts; gastrointestinal system; human cell lines; humans; hydrogen bonding; hydrolysates; in vitro studies; inhibitory concentration 50; intestinal absorption; keratinocytes; lungs; molecular models; ostriches; ovalbumin; peptidyl-dipeptidase A; reversed-phase high performance liquid chromatography; sodium hydroxide
Abstract:
... In this research, ovalbumin (OOW), one of the major components in ostrich egg white, was purified by anion exchange chromatography. Then, the purified OOW was subjected to alkaline hydrolysis (0.25 M NaOH) at 40 °C for 2–10 h. The best angiotensin I-converting enzyme (ACE) inhibitory activity was observed at 8 h of hydrolysis. The OOW hydrolysate obtained at 8 h (8 h-hOOW) was purified by the reve ...
DOI:
10.1016/j.procbio.2018.07.014

2. A novel enzymatic method for the synthesis of methyl 6-O-acetyl-α-d-glucopyranoside using a fermented solid containing lipases produced by Burkholderia contaminans LTEB11

Author:
Villalobos, Maribel Cordero; Gonçalves, Alan Guilherme; Noseda, Miguel Daniel; Mitchell, David Alexander; Krieger, Nadia
Source:
Process biochemistry 2018 v.73 pp. 86-93
ISSN:
1359-5113
Subject:
Burkholderia; acetylation; biocatalysts; carbohydrates; catalytic activity; enzymatic treatment; high performance liquid chromatography; hydrophobicity; nuclear magnetic resonance spectroscopy; regioselectivity; solvents; triacylglycerol lipase; vinyl acetate
Abstract:
... Chemical methods for the regioselective modification of carbohydrates require several steps that involve the addition and removal of protective groups. Enzymatic methods are promising since they can give high selectivity in a single step. We developed a new method for acetylation of methyl-α-d-glucopyranoside with vinyl acetate, the reaction being catalyzed by a fermented solid containing lipases ...
DOI:
10.1016/j.procbio.2018.07.023

3. A prolyl endopeptidase from Flammulina velutipes for the possible degradation of celiac disease provoking toxic peptides in cereal proteins

Author:
Schulz, Kathrin; Giesler, Lucienne; Linke, Diana; Berger, Ralf G.
Source:
Process biochemistry 2018 v.73 pp. 47-55
ISSN:
1359-5113
Subject:
Aspergillus niger; Aspergillus oryzae; EDTA (chelating agent); Flammulina velutipes; calcium chloride; casein; celiac disease; cleavage (chemistry); cobalt; copper; enzyme activity; epitopes; foods; gelatin; genes; gliadin; gluten; iron; isoelectric point; manganese; molecular weight; nickel; pH; patients; peptides; prolyl oligopeptidase; reversed-phase high performance liquid chromatography; sequence homology; toxicity; wheat; zinc
Abstract:
... A prolyl endopeptidase, FvpP, was identified in the culture supernatant of the basidiomycete Flammulina velutipes and functionally expressed for gene verification in Aspergillus oryzae. The wild-type FvpP with a molecular mass of 50 kDa under denaturing conditions, exhibited optima at pH 5, and 45 °C and an isoelectric point of 3.8. Five mM Fe²⁺ and Fe³⁺ activated the prolyl endopeptidase, while i ...
DOI:
10.1016/j.procbio.2018.07.019

4. Biological and anticancer properties of Inonotus obliquus extracts

Author:
Szychowski, Konrad A.; Rybczyńska-Tkaczyk, Kamila; Tobiasz, Jakub; Yelnytska-Stawasz, Viktoriya; Pomianek, Tadeusz; Gmiński, Jan
Source:
Process biochemistry 2018 v.73 pp. 180-187
ISSN:
1359-5113
Subject:
Inonotus obliquus; adenocarcinoma; antineoplastic activity; antioxidant activity; cardiovascular diseases; catalase; cell culture; cell viability; colorectal neoplasms; cytotoxicity; diabetes; ethanol; fibroblasts; free radical scavengers; gastrointestinal neoplasms; gene expression; human cell lines; mechanism of action; messenger RNA; methanol; neoplasm cells; skin (animal); superoxide dismutase; traditional medicine; xanthine oxidase
Abstract:
... Inonotus obliquus (IO) has traditionally been used in folk medicine in the treatment of gastrointestinal cancer, cardiovascular disease and diabetes. The aim of our study was to evaluate the biological and metabolic properties of IO extracts. Free radical scavenging properties, inhibition of the activity of xanthine oxidase (XOi), induction of reactive oxygen species (ROS) production, cell viabili ...
DOI:
10.1016/j.procbio.2018.07.015

5. Biotransformation of 4-hydroxyphenylacetonitrile to 4-hydroxyphenylacetic acid using whole cell arylacetonitrilase of Alcaligenes faecalis MTCC 12629

Author:
Thakur, Neerja; Kumar, Vijay; Thakur, Shikha; Sharma, Nikhil; Sheetal,; Bhalla, Tek Chand
Source:
Process biochemistry 2018 v.73 pp. 117-123
ISSN:
1359-5113
Subject:
Alcaligenes faecalis; acids; batch systems; bioprocessing; biotransformation; buffers; half life; nitrilase; pH; thermal stability
Abstract:
... The whole cell arylacetonitrilase of Alcaligenes faecalis MTCC 12629 was employed to develop a bioprocess for the synthesis of 4-hydroxyphenylacetic acid (4-HPAA) from 4-hydroxyphenylacetonitrile (4-HPAN). Isobutyronitrile emerged as the most appropriate inducer with a 1.2-fold enhanced nitrilase production in inducer feeding approach. A. faecalis MTCC 12629 nitrilase preferably hydrolyzed 4-HPAN, ...
DOI:
10.1016/j.procbio.2018.07.012

6. Characterization and minimization of sialic acid degradation in an Fc-fusion protein-producing CHO cell bioprocess

Author:
Chen, Xinning; Liu, Jintao; Liu, Xuping; Fan, Li; Zhao, Liang; Tan, Wen-Song
Source:
Process biochemistry 2018 v.73 pp. 162-169
ISSN:
1359-5113
Subject:
bioactive properties; bioprocessing; bioreactors; cell culture; glycoproteins; half life; monosaccharides; pH; polysaccharides; process control; protein synthesis; sialic acid; sialidase; therapeutics
Abstract:
... Sialic acid content generally affects many biochemical properties of therapeutic glycoproteins. More specific properties, such as charge distribution, circulating half-life and bioactivity, are directly impacted by sialic acid content. Undesirable loss of protein sialylation is commonly observed in CHO bioprocesses, and sialic acid degradation is one of the root causes. In this work, sialic acid d ...
DOI:
10.1016/j.procbio.2018.08.015

7. Characterization of an exopolysaccharide produced by Lactobacillus plantarum HM47 isolated from human breast milk

Author:
Nambiar, Reshma B; Sellamuthu, Periyar Selvam; Perumal, Anand Babu; Sadiku, Emmanuel Rotimi; Phiri, Goitse; Jayaramudu, J.
Source:
Process biochemistry 2018 v.73 pp. 15-22
ISSN:
1359-5113
Subject:
Fourier transform infrared spectroscopy; Lactobacillus plantarum; breast milk; energy-dispersive X-ray analysis; exopolysaccharides; food industry; galactose; glucose; moieties; nuclear magnetic resonance spectroscopy; polymers; scanning electron microscopy; sodium; temperature; thermal analysis; thermal stability
Abstract:
... In the present study, exopolysaccharide (EPS) secreted by Lactobacillus plantarum HM47, was purified and characterized. The structural characterization of EPS revealed that it was a polymer of glucose, and galactose in the ratio of 0.5:1.4. The FTIR spectroscopy showed the prevalence of carboxyl and hydroxyl groups. ¹H NMR and ¹³C NMR spectra analysis showed the presence of β-d-glucose and β-d-gal ...
DOI:
10.1016/j.procbio.2018.07.018

8. Comparison of performance and microbial communities in a bioelectrochemical system for simultaneous denitrification and chromium removal: Effects of pH

Author:
Wang, Hongyu; Zhang, Shilu; Wang, Jing; Song, Qun; Zhang, Wei; He, Qiulai; Song, Jianyang; Ma, Fang
Source:
Process biochemistry 2018 v.73 pp. 154-161
ISSN:
1359-5113
Subject:
Halomonas; Pseudomonas; Thauera; bacterial communities; bioelectrochemistry; chromium; denitrification; denitrifying bacteria; nitrates; pH; species diversity
Abstract:
... A novel bioelectrochemical system (BES) was established to investigate the feasibility of simultaneous autotrophic denitrification and chromium removal under different pH conditions in terms of the removal performance and dominant microbial community. Reliable nitrate and hexavalent chromium (Cr (VI)) removal was obtained, and the optimum pH was 7.0 with the highest removal efficiencies of 97.21 a ...
DOI:
10.1016/j.procbio.2018.08.007

9. Construction of a food-grade arginase expression system and its application in L-ornithine production with whole cell biocatalyst

Author:
Kai, Huang; Xiao, Guan; Bo, Jiang; Sen, Li
Source:
Process biochemistry 2018 v.73 pp. 94-101
ISSN:
1359-5113
Subject:
Bacillus subtilis; antibiotic resistance genes; arginase; arginine; biocatalysts; biotransformation; chromosomes; culture media; enzyme activity; gene overexpression; manganese; ornithine carbamoyltransferase; pH; plasmids
Abstract:
... A food-grade recombinant Bacillus subtilis overexpressing arginase from Rummeliibacillus pycnus was successfully constructed based on replicative plasmids. D-Alanine racemase gene on the host chromosome was knocked out with Cre/lox system. Replacement of antibiotic resistance gene with dal in the plasmids would complement the deletion of the host strain and offer a selective pressure to keep plasm ...
DOI:
10.1016/j.procbio.2018.07.020

10. Different glyceollin synthesis-related metabolic content and gene expressions in soybean callus suspension cultures and cotyledon tissues induced by alginate oligosaccharides

Author:
Wang, Kaiqiang; Peng, Qing; Qiao, Yu; Li, Yang; Suo, Decheng; Shi, Bo
Source:
Process biochemistry 2018 v.73 pp. 188-196
ISSN:
1359-5113
Subject:
Glycine max; alginates; biosynthesis; callus; cotyledons; daidzein; gene expression; genes; genistein; glycitein; high performance liquid chromatography; isomers; metabolites; naringenin; oligosaccharides; phytoalexins; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; soybeans
Abstract:
... Glyceollins are a group of inducible soybean phytoalexins. The aim of the present study was to investigate the mechanism of glyceollin production in callus suspension cultures (CS) and cotyledon tissues elicited with alginate oligosaccharides (AOS) at 1%, 2%, and 3% concentrations that were applied for three days. HPLC analysis of the extracts from 2% AOS-elicited callus suspension cultures (ECS) ...
DOI:
10.1016/j.procbio.2018.08.013

11. Dual column approach for the purification of zinc finger proteins by immobilized metal affinity chromatography

Author:
Ko, Ching-Chung; Ostermeier, Marc; Lin, Sung-Chyr
Source:
Process biochemistry 2018 v.73 pp. 204-210
ISSN:
1359-5113
Subject:
adsorbents; adsorption; affinity chromatography; cobalt; copper; dialysis; metal ions; models; nickel; pH; recombinant proteins; sodium chloride; zinc; zinc finger motif
Abstract:
... The feasibility of using zinc fingers as the affinity tag for the purification of recombinant proteins with immobilized metal affinity chromatography (IMAC) was investigated. It was shown that upon the release of pre-existing metal ions from the model zinc-finger fusion protein by dialysis with buffer containing 500 mM NaCl, the recovery of the zinc-finger fusion protein was increased from 47% to ...
DOI:
10.1016/j.procbio.2018.08.025

12. Effect of particle size and enzyme load on the simultaneous reactions of lactose hydrolysis and transgalactosylation with glyoxyl-agarose immobilized β-galactosidase from Aspergillus oryzae

Author:
Suárez, Sebastián; Guerrero, Cecilia; Vera, Carlos; Illanes, Andrés
Source:
Process biochemistry 2018 v.73 pp. 56-64
ISSN:
1359-5113
Subject:
Aspergillus oryzae; beta-galactosidase; biocatalysts; galactooligosaccharides; hydrolysis; lactose; particle size
Abstract:
... The simultaneous reactions of lactose hydrolysis and transgalactosylation in the production of galacto-oligosaccharides (GOS) were evaluated with immobilized Aspergillus oryzae β-galactosidase in glyoxyl-agarose of different particle sizes (fine and macro) and enzyme loads (1, 10 and 30 mgprotein/gsupport) to produce biocatalysts subjected to different magnitudes of internal diffusional restrictio ...
DOI:
10.1016/j.procbio.2018.08.016

13. Effect of starch liberation method and initial pH of sweet mashes on higher alcohols content in distillates obtained from different starchy raw materials

Author:
Pielech-Przybylska, Katarzyna; Balcerek, Maria; Dziekońska-Kubczak, Urszula; Patelski, Piotr; Różański, Mateusz
Source:
Process biochemistry 2018 v.73 pp. 29-37
ISSN:
1359-5113
Subject:
1-propanol; butanol; distillates; ethanol fermentation; isopropyl alcohol; mash; pH; phenylethyl alcohol; raw materials; starch; volatile compounds; yeasts
Abstract:
... The qualitative and quantitative profile of volatile compounds in agricultural distillate depends on numerous factors. Many such compounds, including higher alcohols, occur naturally, due to the metabolism of yeast during ethanol fermentation. Some are difficult to remove in the rectification process. For these reasons, it is desirable to reduce the synthesis of volatile compounds, among others hi ...
DOI:
10.1016/j.procbio.2018.08.014

14. Effects of salinity on nitrification efficiency and bacterial community structure in a nitrifying osmotic membrane bioreactor

Author:
Jeong, Dawoon; Cho, Kyungjin; Lee, Chang-Ha; Lee, Seockheon; Bae, Hyokwan
Source:
Process biochemistry 2018 v.73 pp. 132-141
ISSN:
1359-5113
Subject:
Nitrobacter winogradskyi; Nitrosomonas eutropha; alkalinity; ammonia; bacterial communities; carbon; cluster analysis; community structure; genes; membrane bioreactors; multidimensional scaling; nitrates; nitrification; nitrifying bacteria; nitrites; nitrogen-fixing bacteria; osmosis; pollution load; salinity; salt tolerance; sequence analysis; total dissolved solids
Abstract:
... The objective of this study was to evaluate the effects of salt accumulation on nitrifying bacterial communities in a nitrifying bioreactor combined with forward osmosis. The conversion of nitrite to nitrate was inhibited at a total dissolved solids (TDS) concentration of 17.3 g/L, whereas conversion of ammonia to nitrite was inhibited at a higher concentration (52.8 g-TDS/L). The gene copies of a ...
DOI:
10.1016/j.procbio.2018.08.008

15. Efficient carbon dioxide sequestration by using recombinant carbonic anhydrase

Author:
Tan, Shih-I; Han, Yin-Lung; Yu, You-Jin; Chiu, Chen-Yaw; Chang, Yu-Kaung; Ouyang, Shoung; Fan, Kai-Chun; Lo, Kuei-Ho; Ng, I-Son
Source:
Process biochemistry 2018 v.73 pp. 38-46
ISSN:
1359-5113
Subject:
Escherichia coli; Mesorhizobium loti; agar; ammonium; biocatalysts; calcium; calcium carbonate; carbon dioxide; carbon sequestration; carbonate dehydratase; catalytic activity; climate change; cobalt; copper; diffusivity; magnesium; manganese; pH; potassium; salinity; sodium; zinc
Abstract:
... Efficient biological carbon dioxide sequestration for slowing down the climate changes requires a highly active and stable carbonic anhydrase (CA). A CA from Mesorhizobium loti (i.e., MlCA) was first over-expressed in E. coli via different vectors and a concentration of up to 5 g/L was obtained with pET32a(+). The whole cell biocatalyst was stable between pH 4.0 and pH 9.0 and maintained 82% activ ...
DOI:
10.1016/j.procbio.2018.08.017

16. Efficient conversion of chitosan into chitooligosaccharides by a chitosanolytic activity from Bacillus thuringiensis

Author:
Santos-Moriano, Paloma; Kidibule, Peter E.; Alleyne, Elena; Ballesteros, Antonio O.; Heras, Angeles; Fernandez-Lobato, Maria; Plou, Francisco J.
Source:
Process biochemistry 2018 v.73 pp. 102-108
ISSN:
1359-5113
Subject:
Bacillus thuringiensis; anion exchange chromatography; biopesticides; chitooligosaccharides; chitosan; mass spectrometry; pH; sodium acetate; temperature; washing
Abstract:
... Starting from a commercial preparation of Bacillus thuringiensis var. aizawai, widely employed as biopesticide in agriculture, we obtained a chitosanolytic activity by washing the cells with concentrated sodium acetate. The optimum pH and temperature values for this enzyme were 6.0 and 60 °C, respectively. The enzymatic extract efficiently hydrolyzed various chitosans with different deacetylation ...
DOI:
10.1016/j.procbio.2018.07.017

17. Exploring the gut of Helicoverpa armigera for cellulose degrading bacteria and evaluation of a potential strain for lignocellulosic biomass deconstruction

Author:
Dar, Mudasir A.; Shaikh, Afrin A.; Pawar, Kiran D.; Pandit, Radhakrishna S.
Source:
Process biochemistry 2018 v.73 pp. 142-153
ISSN:
1359-5113
Subject:
Fourier transform infrared spectroscopy; Helicoverpa armigera; Klebsiella; X-ray diffraction; bacteria; beta-glucosidase; biomass; biorefining; cellulolytic microorganisms; cellulose; cellulosic wastes; crystal structure; digestive system; dust; enzyme activity; hydrolysis; lignocellulose; pulp and paper industry; reducing sugars; ribosomal RNA; sawdust; scanning electron microscopy; sequence analysis; spectral analysis; xylanases
Abstract:
... The present study demonstrates the enrichment, isolation and identification of cellulose degrading bacteria from the gut of cotton bollworm, Helicoverpa armigera. The isolate MD21 was identified as Klebsiella sp. MD21 (MG367463) based on 16S rRNA gene sequencing. The isolated bacterium, Klebsiella sp. MD21 secreted many hydrolytic enzymes particularly xylanase and β-glucosidase with highest activi ...
DOI:
10.1016/j.procbio.2018.08.001

18. Extraction, purification, and characterization of polysaccharides from marine algae Gracilaria lemaneiformis with anti-tumor activity

Author:
Shi, Fulin; Yan, Xiaolin; Cheong, Kit-Leong; Liu, Yang
Source:
Process biochemistry 2018 v.73 pp. 197-203
ISSN:
1359-5113
Subject:
Gracilaria; adjuvants; algae; ammonium sulfate; antineoplastic activity; cell proliferation; chemical structure; ethanol; galactose; human cell lines; molecular weight; polysaccharides; purification methods; response surface methodology; temperature; ultrasonics
Abstract:
... Recently, rapid and effective extraction and purification methods have attracted considerable interest as alternative approaches to conventional methods. In this study, ultrasonic-microwave-assisted extraction (UMAE) was used to extract Gracilaria lemaneiformis polysaccharides, and the response surface methodology was used to determine the optimum extraction conditions. Subsequently, an aqueous tw ...
DOI:
10.1016/j.procbio.2018.08.011

19. High cell density cultivation of Δgor/ΔtrxB E. coli in a chemically defined minimal medium with an enhanced iron concentration

Author:
Joachim, Mathias; Schäfer, Johannes Gregor; Gerlach, Doreen; Czermak, Peter
Source:
Process biochemistry 2018 v.73 pp. 1-5
ISSN:
1359-5113
Subject:
Escherichia coli; cytoplasm; disulfide bonds; genetic engineering; glucose; glutathione-disulfide reductase; inclusion bodies; iron; isomerases; thioredoxins
Abstract:
... In Escherichia coli, the production of active disulfide bonds is achieved by expression into the oxidizing periplasm, by refolding from inclusion bodies, by co-expression of a sulfhydryl oxidase and a disulfide bond isomerase, or by expression into a genetically engineered oxidizing cytoplasm. Thus, engineered strains with oxidizing cytoplasm were grown in complex media. For a large scale producti ...
DOI:
10.1016/j.procbio.2018.07.022

20. Immobilization of β-galactosidase in glutaraldehyde-chitosan and its application to the synthesis of lactulose using cheese whey as feedstock

Author:
de Albuquerque, Tiago Lima; Gomes, Sandy Danielle Lucindo; D’Almeida, Alan Portal; Fernandez-Lafuente, Roberto; Gonçalves, Luciana Rocha Barros; Rocha, Maria Valderez Ponte
Source:
Process biochemistry 2018 v.73 pp. 65-73
ISSN:
1359-5113
Subject:
Kluyveromyces marxianus var. lactis; beta-galactosidase; catalysts; cheese whey; chitosan; feedstocks; fructose; glutaraldehyde; lactose; lactulose; magnesium; pH; wastes
Abstract:
... The aim of this work was to establish optimal conditions for the enzymatic production of lactulose by β-galactosidase from Kluyveromyces lactis using cheese whey as feedstock. Initially, the production of this disaccharide was evaluated using free β-galactosidase and synthetic medium (containing lactose and fructose), and the optimal conditions for the production of lactulose were as follows: lact ...
DOI:
10.1016/j.procbio.2018.08.010