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- Author:
- Khueychai, Siriporn; Jangpromma, Nisachon; Choowongkomon, Kiattawee; Joompang, Anupong; Daduang, Sakda; Vesaratchavest, Mongkol; Payoungkiattikun, Wisarut; Tachibana, Shinjiro; Klaynongsruang, Sompong
- Source:
- Process biochemistry 2018 v.73 pp. 235-245
- ISSN:
- 1359-5113
- Subject:
- Struthio camelus; absorption; alkaline hydrolysis; anion exchange chromatography; cytotoxicity; digestion; egg albumen; erythrocytes; fibroblasts; gastrointestinal system; human cell lines; humans; hydrogen bonding; hydrolysates; in vitro studies; inhibitory concentration 50; intestinal absorption; keratinocytes; lungs; molecular models; ostriches; ovalbumin; peptidyl-dipeptidase A; reversed-phase high performance liquid chromatography; sodium hydroxide
- Abstract:
- ... In this research, ovalbumin (OOW), one of the major components in ostrich egg white, was purified by anion exchange chromatography. Then, the purified OOW was subjected to alkaline hydrolysis (0.25 M NaOH) at 40 °C for 2–10 h. The best angiotensin I-converting enzyme (ACE) inhibitory activity was observed at 8 h of hydrolysis. The OOW hydrolysate obtained at 8 h (8 h-hOOW) was purified by the reve ...
- DOI:
- 10.1016/j.procbio.2018.07.014
-
https://dx.doi.org/10.1016/j.procbio.2018.07.014
- Author:
- Villalobos, Maribel Cordero; Gonçalves, Alan Guilherme; Noseda, Miguel Daniel; Mitchell, David Alexander; Krieger, Nadia
- Source:
- Process biochemistry 2018 v.73 pp. 86-93
- ISSN:
- 1359-5113
- Subject:
- Burkholderia; acetylation; biocatalysts; carbohydrates; catalytic activity; enzymatic treatment; high performance liquid chromatography; hydrophobicity; nuclear magnetic resonance spectroscopy; regioselectivity; solvents; triacylglycerol lipase; vinyl acetate
- Abstract:
- ... Chemical methods for the regioselective modification of carbohydrates require several steps that involve the addition and removal of protective groups. Enzymatic methods are promising since they can give high selectivity in a single step. We developed a new method for acetylation of methyl-α-d-glucopyranoside with vinyl acetate, the reaction being catalyzed by a fermented solid containing lipases ...
- DOI:
- 10.1016/j.procbio.2018.07.023
-
https://dx.doi.org/10.1016/j.procbio.2018.07.023
- Author:
- Schulz, Kathrin; Giesler, Lucienne; Linke, Diana; Berger, Ralf G.
- Source:
- Process biochemistry 2018 v.73 pp. 47-55
- ISSN:
- 1359-5113
- Subject:
- Aspergillus niger; Aspergillus oryzae; EDTA (chelating agent); Flammulina velutipes; calcium chloride; casein; celiac disease; cleavage (chemistry); cobalt; copper; enzyme activity; epitopes; foods; gelatin; genes; gliadin; gluten; iron; isoelectric point; manganese; molecular weight; nickel; pH; patients; peptides; prolyl oligopeptidase; reversed-phase high performance liquid chromatography; sequence homology; toxicity; wheat; zinc
- Abstract:
- ... A prolyl endopeptidase, FvpP, was identified in the culture supernatant of the basidiomycete Flammulina velutipes and functionally expressed for gene verification in Aspergillus oryzae. The wild-type FvpP with a molecular mass of 50 kDa under denaturing conditions, exhibited optima at pH 5, and 45 °C and an isoelectric point of 3.8. Five mM Fe²⁺ and Fe³⁺ activated the prolyl endopeptidase, while i ...
- DOI:
- 10.1016/j.procbio.2018.07.019
-
https://dx.doi.org/10.1016/j.procbio.2018.07.019
- Author:
- Szychowski, Konrad A.; Rybczyńska-Tkaczyk, Kamila; Tobiasz, Jakub; Yelnytska-Stawasz, Viktoriya; Pomianek, Tadeusz; Gmiński, Jan
- Source:
- Process biochemistry 2018 v.73 pp. 180-187
- ISSN:
- 1359-5113
- Subject:
- Inonotus obliquus; adenocarcinoma; antineoplastic activity; antioxidant activity; cardiovascular diseases; catalase; cell culture; cell viability; colorectal neoplasms; cytotoxicity; diabetes; ethanol; fibroblasts; free radical scavengers; gastrointestinal neoplasms; gene expression; human cell lines; mechanism of action; messenger RNA; methanol; neoplasm cells; skin (animal); superoxide dismutase; traditional medicine; xanthine oxidase
- Abstract:
- ... Inonotus obliquus (IO) has traditionally been used in folk medicine in the treatment of gastrointestinal cancer, cardiovascular disease and diabetes. The aim of our study was to evaluate the biological and metabolic properties of IO extracts. Free radical scavenging properties, inhibition of the activity of xanthine oxidase (XOi), induction of reactive oxygen species (ROS) production, cell viabili ...
- DOI:
- 10.1016/j.procbio.2018.07.015
-
https://dx.doi.org/10.1016/j.procbio.2018.07.015
- Author:
- Thakur, Neerja; Kumar, Vijay; Thakur, Shikha; Sharma, Nikhil; Sheetal,; Bhalla, Tek Chand
- Source:
- Process biochemistry 2018 v.73 pp. 117-123
- ISSN:
- 1359-5113
- Subject:
- Alcaligenes faecalis; acids; batch systems; bioprocessing; biotransformation; buffers; half life; nitrilase; pH; thermal stability
- Abstract:
- ... The whole cell arylacetonitrilase of Alcaligenes faecalis MTCC 12629 was employed to develop a bioprocess for the synthesis of 4-hydroxyphenylacetic acid (4-HPAA) from 4-hydroxyphenylacetonitrile (4-HPAN). Isobutyronitrile emerged as the most appropriate inducer with a 1.2-fold enhanced nitrilase production in inducer feeding approach. A. faecalis MTCC 12629 nitrilase preferably hydrolyzed 4-HPAN, ...
- DOI:
- 10.1016/j.procbio.2018.07.012
-
https://dx.doi.org/10.1016/j.procbio.2018.07.012
- Author:
- Chen, Xinning; Liu, Jintao; Liu, Xuping; Fan, Li; Zhao, Liang; Tan, Wen-Song
- Source:
- Process biochemistry 2018 v.73 pp. 162-169
- ISSN:
- 1359-5113
- Subject:
- bioactive properties; bioprocessing; bioreactors; cell culture; glycoproteins; half life; monosaccharides; pH; polysaccharides; process control; protein synthesis; sialic acid; sialidase; therapeutics
- Abstract:
- ... Sialic acid content generally affects many biochemical properties of therapeutic glycoproteins. More specific properties, such as charge distribution, circulating half-life and bioactivity, are directly impacted by sialic acid content. Undesirable loss of protein sialylation is commonly observed in CHO bioprocesses, and sialic acid degradation is one of the root causes. In this work, sialic acid d ...
- DOI:
- 10.1016/j.procbio.2018.08.015
-
https://dx.doi.org/10.1016/j.procbio.2018.08.015
- Author:
- Nambiar, Reshma B; Sellamuthu, Periyar Selvam; Perumal, Anand Babu; Sadiku, Emmanuel Rotimi; Phiri, Goitse; Jayaramudu, J.
- Source:
- Process biochemistry 2018 v.73 pp. 15-22
- ISSN:
- 1359-5113
- Subject:
- Fourier transform infrared spectroscopy; Lactobacillus plantarum; breast milk; energy-dispersive X-ray analysis; exopolysaccharides; food industry; galactose; glucose; moieties; nuclear magnetic resonance spectroscopy; polymers; scanning electron microscopy; sodium; temperature; thermal analysis; thermal stability
- Abstract:
- ... In the present study, exopolysaccharide (EPS) secreted by Lactobacillus plantarum HM47, was purified and characterized. The structural characterization of EPS revealed that it was a polymer of glucose, and galactose in the ratio of 0.5:1.4. The FTIR spectroscopy showed the prevalence of carboxyl and hydroxyl groups. ¹H NMR and ¹³C NMR spectra analysis showed the presence of β-d-glucose and β-d-gal ...
- DOI:
- 10.1016/j.procbio.2018.07.018
-
https://dx.doi.org/10.1016/j.procbio.2018.07.018
- Author:
- Wang, Hongyu; Zhang, Shilu; Wang, Jing; Song, Qun; Zhang, Wei; He, Qiulai; Song, Jianyang; Ma, Fang
- Source:
- Process biochemistry 2018 v.73 pp. 154-161
- ISSN:
- 1359-5113
- Subject:
- Halomonas; Pseudomonas; Thauera; bacterial communities; bioelectrochemistry; chromium; denitrification; denitrifying bacteria; nitrates; pH; species diversity
- Abstract:
- ... A novel bioelectrochemical system (BES) was established to investigate the feasibility of simultaneous autotrophic denitrification and chromium removal under different pH conditions in terms of the removal performance and dominant microbial community. Reliable nitrate and hexavalent chromium (Cr (VI)) removal was obtained, and the optimum pH was 7.0 with the highest removal efficiencies of 97.21 a ...
- DOI:
- 10.1016/j.procbio.2018.08.007
-
https://dx.doi.org/10.1016/j.procbio.2018.08.007
- Author:
- Kai, Huang; Xiao, Guan; Bo, Jiang; Sen, Li
- Source:
- Process biochemistry 2018 v.73 pp. 94-101
- ISSN:
- 1359-5113
- Subject:
- Bacillus subtilis; antibiotic resistance genes; arginase; arginine; biocatalysts; biotransformation; chromosomes; culture media; enzyme activity; gene overexpression; manganese; ornithine carbamoyltransferase; pH; plasmids
- Abstract:
- ... A food-grade recombinant Bacillus subtilis overexpressing arginase from Rummeliibacillus pycnus was successfully constructed based on replicative plasmids. D-Alanine racemase gene on the host chromosome was knocked out with Cre/lox system. Replacement of antibiotic resistance gene with dal in the plasmids would complement the deletion of the host strain and offer a selective pressure to keep plasm ...
- DOI:
- 10.1016/j.procbio.2018.07.020
-
https://dx.doi.org/10.1016/j.procbio.2018.07.020
- Author:
- Wang, Kaiqiang; Peng, Qing; Qiao, Yu; Li, Yang; Suo, Decheng; Shi, Bo
- Source:
- Process biochemistry 2018 v.73 pp. 188-196
- ISSN:
- 1359-5113
- Subject:
- Glycine max; alginates; biosynthesis; callus; cotyledons; daidzein; gene expression; genes; genistein; glycitein; high performance liquid chromatography; isomers; metabolites; naringenin; oligosaccharides; phytoalexins; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; soybeans
- Abstract:
- ... Glyceollins are a group of inducible soybean phytoalexins. The aim of the present study was to investigate the mechanism of glyceollin production in callus suspension cultures (CS) and cotyledon tissues elicited with alginate oligosaccharides (AOS) at 1%, 2%, and 3% concentrations that were applied for three days. HPLC analysis of the extracts from 2% AOS-elicited callus suspension cultures (ECS) ...
- DOI:
- 10.1016/j.procbio.2018.08.013
-
https://dx.doi.org/10.1016/j.procbio.2018.08.013
- Author:
- Ko, Ching-Chung; Ostermeier, Marc; Lin, Sung-Chyr
- Source:
- Process biochemistry 2018 v.73 pp. 204-210
- ISSN:
- 1359-5113
- Subject:
- adsorbents; adsorption; affinity chromatography; cobalt; copper; dialysis; metal ions; models; nickel; pH; recombinant proteins; sodium chloride; zinc; zinc finger motif
- Abstract:
- ... The feasibility of using zinc fingers as the affinity tag for the purification of recombinant proteins with immobilized metal affinity chromatography (IMAC) was investigated. It was shown that upon the release of pre-existing metal ions from the model zinc-finger fusion protein by dialysis with buffer containing 500 mM NaCl, the recovery of the zinc-finger fusion protein was increased from 47% to ...
- DOI:
- 10.1016/j.procbio.2018.08.025
-
https://dx.doi.org/10.1016/j.procbio.2018.08.025
- Author:
- Suárez, Sebastián; Guerrero, Cecilia; Vera, Carlos; Illanes, Andrés
- Source:
- Process biochemistry 2018 v.73 pp. 56-64
- ISSN:
- 1359-5113
- Subject:
- Aspergillus oryzae; beta-galactosidase; biocatalysts; galactooligosaccharides; hydrolysis; lactose; particle size
- Abstract:
- ... The simultaneous reactions of lactose hydrolysis and transgalactosylation in the production of galacto-oligosaccharides (GOS) were evaluated with immobilized Aspergillus oryzae β-galactosidase in glyoxyl-agarose of different particle sizes (fine and macro) and enzyme loads (1, 10 and 30 mgprotein/gsupport) to produce biocatalysts subjected to different magnitudes of internal diffusional restrictio ...
- DOI:
- 10.1016/j.procbio.2018.08.016
-
https://dx.doi.org/10.1016/j.procbio.2018.08.016
- Author:
- Pielech-Przybylska, Katarzyna; Balcerek, Maria; Dziekońska-Kubczak, Urszula; Patelski, Piotr; Różański, Mateusz
- Source:
- Process biochemistry 2018 v.73 pp. 29-37
- ISSN:
- 1359-5113
- Subject:
- 1-propanol; butanol; distillates; ethanol fermentation; isopropyl alcohol; mash; pH; phenylethyl alcohol; raw materials; starch; volatile compounds; yeasts
- Abstract:
- ... The qualitative and quantitative profile of volatile compounds in agricultural distillate depends on numerous factors. Many such compounds, including higher alcohols, occur naturally, due to the metabolism of yeast during ethanol fermentation. Some are difficult to remove in the rectification process. For these reasons, it is desirable to reduce the synthesis of volatile compounds, among others hi ...
- DOI:
- 10.1016/j.procbio.2018.08.014
-
https://dx.doi.org/10.1016/j.procbio.2018.08.014
- Author:
- Jeong, Dawoon; Cho, Kyungjin; Lee, Chang-Ha; Lee, Seockheon; Bae, Hyokwan
- Source:
- Process biochemistry 2018 v.73 pp. 132-141
- ISSN:
- 1359-5113
- Subject:
- Nitrobacter winogradskyi; Nitrosomonas eutropha; alkalinity; ammonia; bacterial communities; carbon; cluster analysis; community structure; genes; membrane bioreactors; multidimensional scaling; nitrates; nitrification; nitrifying bacteria; nitrites; nitrogen-fixing bacteria; osmosis; pollution load; salinity; salt tolerance; sequence analysis; total dissolved solids
- Abstract:
- ... The objective of this study was to evaluate the effects of salt accumulation on nitrifying bacterial communities in a nitrifying bioreactor combined with forward osmosis. The conversion of nitrite to nitrate was inhibited at a total dissolved solids (TDS) concentration of 17.3 g/L, whereas conversion of ammonia to nitrite was inhibited at a higher concentration (52.8 g-TDS/L). The gene copies of a ...
- DOI:
- 10.1016/j.procbio.2018.08.008
-
https://dx.doi.org/10.1016/j.procbio.2018.08.008
- Author:
- Tan, Shih-I; Han, Yin-Lung; Yu, You-Jin; Chiu, Chen-Yaw; Chang, Yu-Kaung; Ouyang, Shoung; Fan, Kai-Chun; Lo, Kuei-Ho; Ng, I-Son
- Source:
- Process biochemistry 2018 v.73 pp. 38-46
- ISSN:
- 1359-5113
- Subject:
- Escherichia coli; Mesorhizobium loti; agar; ammonium; biocatalysts; calcium; calcium carbonate; carbon dioxide; carbon sequestration; carbonate dehydratase; catalytic activity; climate change; cobalt; copper; diffusivity; magnesium; manganese; pH; potassium; salinity; sodium; zinc
- Abstract:
- ... Efficient biological carbon dioxide sequestration for slowing down the climate changes requires a highly active and stable carbonic anhydrase (CA). A CA from Mesorhizobium loti (i.e., MlCA) was first over-expressed in E. coli via different vectors and a concentration of up to 5 g/L was obtained with pET32a(+). The whole cell biocatalyst was stable between pH 4.0 and pH 9.0 and maintained 82% activ ...
- DOI:
- 10.1016/j.procbio.2018.08.017
-
https://dx.doi.org/10.1016/j.procbio.2018.08.017
- Author:
- Santos-Moriano, Paloma; Kidibule, Peter E.; Alleyne, Elena; Ballesteros, Antonio O.; Heras, Angeles; Fernandez-Lobato, Maria; Plou, Francisco J.
- Source:
- Process biochemistry 2018 v.73 pp. 102-108
- ISSN:
- 1359-5113
- Subject:
- Bacillus thuringiensis; anion exchange chromatography; biopesticides; chitooligosaccharides; chitosan; mass spectrometry; pH; sodium acetate; temperature; washing
- Abstract:
- ... Starting from a commercial preparation of Bacillus thuringiensis var. aizawai, widely employed as biopesticide in agriculture, we obtained a chitosanolytic activity by washing the cells with concentrated sodium acetate. The optimum pH and temperature values for this enzyme were 6.0 and 60 °C, respectively. The enzymatic extract efficiently hydrolyzed various chitosans with different deacetylation ...
- DOI:
- 10.1016/j.procbio.2018.07.017
-
https://dx.doi.org/10.1016/j.procbio.2018.07.017
- Author:
- Dar, Mudasir A.; Shaikh, Afrin A.; Pawar, Kiran D.; Pandit, Radhakrishna S.
- Source:
- Process biochemistry 2018 v.73 pp. 142-153
- ISSN:
- 1359-5113
- Subject:
- Fourier transform infrared spectroscopy; Helicoverpa armigera; Klebsiella; X-ray diffraction; bacteria; beta-glucosidase; biomass; biorefining; cellulolytic microorganisms; cellulose; cellulosic wastes; crystal structure; digestive system; dust; enzyme activity; hydrolysis; lignocellulose; pulp and paper industry; reducing sugars; ribosomal RNA; sawdust; scanning electron microscopy; sequence analysis; spectral analysis; xylanases
- Abstract:
- ... The present study demonstrates the enrichment, isolation and identification of cellulose degrading bacteria from the gut of cotton bollworm, Helicoverpa armigera. The isolate MD21 was identified as Klebsiella sp. MD21 (MG367463) based on 16S rRNA gene sequencing. The isolated bacterium, Klebsiella sp. MD21 secreted many hydrolytic enzymes particularly xylanase and β-glucosidase with highest activi ...
- DOI:
- 10.1016/j.procbio.2018.08.001
-
http://dx.doi.org/10.1016/j.procbio.2018.08.001
- Author:
- Shi, Fulin; Yan, Xiaolin; Cheong, Kit-Leong; Liu, Yang
- Source:
- Process biochemistry 2018 v.73 pp. 197-203
- ISSN:
- 1359-5113
- Subject:
- Gracilaria; adjuvants; algae; ammonium sulfate; antineoplastic activity; cell proliferation; chemical structure; ethanol; galactose; human cell lines; molecular weight; polysaccharides; purification methods; response surface methodology; temperature; ultrasonics
- Abstract:
- ... Recently, rapid and effective extraction and purification methods have attracted considerable interest as alternative approaches to conventional methods. In this study, ultrasonic-microwave-assisted extraction (UMAE) was used to extract Gracilaria lemaneiformis polysaccharides, and the response surface methodology was used to determine the optimum extraction conditions. Subsequently, an aqueous tw ...
- DOI:
- 10.1016/j.procbio.2018.08.011
-
https://dx.doi.org/10.1016/j.procbio.2018.08.011
- Author:
- Joachim, Mathias; Schäfer, Johannes Gregor; Gerlach, Doreen; Czermak, Peter
- Source:
- Process biochemistry 2018 v.73 pp. 1-5
- ISSN:
- 1359-5113
- Subject:
- Escherichia coli; cytoplasm; disulfide bonds; genetic engineering; glucose; glutathione-disulfide reductase; inclusion bodies; iron; isomerases; thioredoxins
- Abstract:
- ... In Escherichia coli, the production of active disulfide bonds is achieved by expression into the oxidizing periplasm, by refolding from inclusion bodies, by co-expression of a sulfhydryl oxidase and a disulfide bond isomerase, or by expression into a genetically engineered oxidizing cytoplasm. Thus, engineered strains with oxidizing cytoplasm were grown in complex media. For a large scale producti ...
- DOI:
- 10.1016/j.procbio.2018.07.022
-
https://dx.doi.org/10.1016/j.procbio.2018.07.022
- Author:
- de Albuquerque, Tiago Lima; Gomes, Sandy Danielle Lucindo; D’Almeida, Alan Portal; Fernandez-Lafuente, Roberto; Gonçalves, Luciana Rocha Barros; Rocha, Maria Valderez Ponte
- Source:
- Process biochemistry 2018 v.73 pp. 65-73
- ISSN:
- 1359-5113
- Subject:
- Kluyveromyces marxianus var. lactis; beta-galactosidase; catalysts; cheese whey; chitosan; feedstocks; fructose; glutaraldehyde; lactose; lactulose; magnesium; pH; wastes
- Abstract:
- ... The aim of this work was to establish optimal conditions for the enzymatic production of lactulose by β-galactosidase from Kluyveromyces lactis using cheese whey as feedstock. Initially, the production of this disaccharide was evaluated using free β-galactosidase and synthetic medium (containing lactose and fructose), and the optimal conditions for the production of lactulose were as follows: lact ...
- DOI:
- 10.1016/j.procbio.2018.08.010
-
https://dx.doi.org/10.1016/j.procbio.2018.08.010