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You searched for: Journal name Process biochemistry Remove constraint Journal name: Process biochemistry Publication year rev 7984-2016 Remove constraint Publication year rev: 7984-2016 Source 2016 v.51 no.9 Remove constraint Source: 2016 v.51 no.9
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1. Anaerobic digestion as a pretreatment to enhance ethanol yield from lignocelluloses

Author:
Mohammad Amin Bahmani; Marzieh Shafiei; Keikhosro Karimi
Source:
Process biochemistry 2016 v.51 no.9 pp. 1256-1263
ISSN:
1359-5113
Subject:
Fourier transform infrared spectroscopy; anaerobic digestion; biogas; ethanol; ethanol production; fermentation; gasoline; hardwood; hemicellulose; hydrolysis; lignocellulose; methane; rice straw; scanning electron microscopy; softwood; ultrasonic treatment
Abstract:
... Biogas production process was used as a biological pretreatment for efficient ethanol production. Three types of lignocelluloses, including rice straw, hardwood sycamore, and softwood pine, were pretreated with anaerobic digestion (AD) process. The effects of ultrasonication on AD-treated and untreated lignocelluloses were studied prior to ethanol production by separate hydrolysis and fermentation ...
DOI:
10.1016/j.procbio.2016.05.012

2. Biophysical analysis of novel oxy-diester hybrid cationic gemini surfactants (Cm-E2O-Cm) with xanthine oxidase (XO)

Author:
Mohd. Akram; Imtiyaz Ahmad Bhat; Sana Anwar; Kabir-ud-Din
Source:
Process biochemistry 2016 v.51 no.9 pp. 1212-1221
ISSN:
1359-5113
Subject:
binding sites; fluorescence; hydrophobicity; milk; molecular models; spectroscopy; surfactants; transmission electron microscopy; xanthine oxidase
Abstract:
... Surfactant–protein/enzyme interactions have gained widespread interest in modern research due to their implications in various domains like pharmaceuticals, cosmetics, paints, biotechnology, etc. Thus, spectroscopic, microscopic and molecular modeling approaches have been employed to explore the interaction of a novel series of oxy-diester hybrid cationic gemini surfactants, 2,2′-[(oxybis(ethane-1 ...
DOI:
10.1016/j.procbio.2016.05.014

3. Chimeric protein of internally duplicated α-type carbonic anhydrase from Dunaliella species for improved expression and CO2 sequestration

Author:
Mi-Ran Ki; Thi Khoa My Nguyen; Sung Ho Kim; Inchan Kwon; Seung Pil Pack
Source:
Process biochemistry 2016 v.51 no.9 pp. 1222-1229
ISSN:
1359-5113
Subject:
Dunaliella; amino acids; calcite; calcium chloride; carbon dioxide; carbon sequestration; carbonate dehydratase; catalytic activity; enzyme activity; genes; recombinant fusion proteins; solubility; thermal stability
Abstract:
... An internally duplicated α-type carbonic anhydrase (CA) from Dunaliella species (Dsp-CA) has been expressed as single-domain derivatives, N- and C- half domains. Although both derivatives have structures similar to that of a known CA (PDB ID: 1y7w), only the C-half domain (Dsp-CA-c) exhibited enzymatic activity, albeit with low solubility. In order to increase the solubility of Dsp-CA-c, its proxi ...
DOI:
10.1016/j.procbio.2016.05.013

4. Chloroperoxidase-catalyzed amino alcohol oxidation: Substrate specificity and novel strategy for the synthesis of N-Cbz-3-aminopropanal

Author:
Gerard Masdeu; Míriam Pérez-Trujillo; Josep López-Santín; Gregorio Álvaro
Source:
Process biochemistry 2016 v.51 no.9 pp. 1204-1211
ISSN:
1359-5113
Subject:
aldehydes; amino alcohols; biotransformation; byproducts; chloride peroxidase; enzyme activity; hydrogen peroxide; oxidation; reaction kinetics; substrate specificity
Abstract:
... The ability of chloroperoxidase (CPO) to catalyze amino alcohol oxidations was investigated. The oxidations of compounds with different configurations with respect to the amine position towards hydroxyl – using H2O2 and tert-butyl hydroperoxide (t-BuOOH) – were analyzed in terms of the initial reaction rate, substrate conversion, and CPO operational stability. It was observed that the further the ...
DOI:
10.1016/j.procbio.2016.05.022

5. Distinctive metabolomic responses of Chlamydomonas reinhardtii to the chemical elicitation by methyl jasmonate and salicylic acid

Author:
Jung-Eun Lee; Yeo Ul Cho; Kyoung Heon Kim; Do Yup Lee
Source:
Process biochemistry 2016 v.51 no.9 pp. 1147-1154
ISSN:
1359-5113
Subject:
Chlamydomonas reinhardtii; algae; alkaloids; amino acid metabolism; amino nitrogen; biosynthesis; carbon; elicitors; fatty acid metabolism; food industry; luminescence; metabolomics; methyl jasmonate; pH; plant hormones; reactive oxygen species; salicylic acid; saturated fatty acids; secondary metabolites; signal transduction
Abstract:
... The microalga-sourced bioactive metabolites are of particular interest related to new product strategy for medicinal, nutritional, cosmetic, and food industries. Conventional approach is to introduce physical stimuli such as pH and luminescence for the algal production. However, considering the specificity and effectiveness, it would be the best way to stimulate secondary metabolism using phytohor ...
DOI:
10.1016/j.procbio.2016.05.029

6. Efficient bioreduction of 2-hydroxyacetophenone to (S)-1-phenyl-1, 2-ethanediol through homologous expression of (S)-carbonyl reductase II in Candida parapsilosis CCTCC M203011

Author:
Yaohui Li; Rongzhen Zhang; Yan Xu; Rong Xiao; Lei Wang; Xiaotian Zhou; Hongbo Liang; Jiawei Jiang
Source:
Process biochemistry 2016 v.51 no.9 pp. 1175-1182
ISSN:
1359-5113
Subject:
Candida parapsilosis; Escherichia coli; alcohol dehydrogenase; alcohols; biocatalysis; biotransformation; molecular cloning; pH
Abstract:
... (S)-carbonyl reductase II (SCRII), a short-chain alcohol dehydrogenase from Candida parapsilosis M203011, catalyzes the bioreduction of 2-hydroxyacetophenone (2-HAP) to (S)-1-phenyl-1,2-ethanediol ((S)-PED). When SCRII was expressed in Escherichia coli, the biotransformation efficiency of (S)-PED was low. To improve its biocatalytic efficiency, the homologous expression of SCRII in C. parapsilosis ...
DOI:
10.1016/j.procbio.2016.05.026

7. Importance of the combined effects of dissolved oxygen and pH on optimization of nitrogen removal in anammox-enriched granular sludge

Author:
Zhixuan Yin; Carla Eloísa Diniz dos Santos; Jaime Gonzalez Vilaplana; Dominika Sobotka; Krzysztof Czerwionka; Márcia Helena Rissato Zamariolli Damianovic; Li Xie; Francisco Jesús Fernández Morales; Jacek Makinia
Source:
Process biochemistry 2016 v.51 no.9 pp. 1274-1282
ISSN:
1359-5113
Subject:
ammonia; anaerobic ammonium oxidation; bacteria; denitrification; dissolved oxygen; nitrites; nitrogen; oxygen; pH; sludge
Abstract:
... The combined effects of dissolved oxygen (DO) and pH on nitrogen removal were investigated in a laboratory-scale sequencing batch reactor (SBR) with anammox-enriched granular sludge obtained from a nitritation/anammox system. The highest specific nitrogen removal rate (SNRR) (1.1gNgVSS⁻¹d⁻¹) was observed under non-aerated conditions, resulting in the nitrogen removal efficiency of 81.6%. Although ...
DOI:
10.1016/j.procbio.2016.05.025

8. In depth understanding the molecular response to the enhanced secretion of fatty acids in Saccharomyces cerevisiae due to one-step gene deletion of acyl-CoA synthetases

Author:
Fang Fang; Bingye Dai; Guang Zhao; Hua Zhao; Chao Sun; Hui Liu; Mo Xian
Source:
Process biochemistry 2016 v.51 no.9 pp. 1162-1174
ISSN:
1359-5113
Subject:
Saccharomyces cerevisiae; acyl coenzyme A; biogenesis; biosynthesis; cell cycle; cell walls; ceramides; ergosterol; fatty acids; free fatty acids; gene deletion; gene expression; gene expression regulation; genes; glycolysis; ligases; messenger RNA; mutants; proteins; secretion; toxicity; yeasts
Abstract:
... Considering of the possible application of fatty acids secretion in industrial production process for reducing extraction costs, the effects of enhanced fatty acids secretion by knocking out acyl-CoA synthetases on the global regulation, cellular metabolism were investigated through integrated omics analysis. Firstly, functional category analysis of differential expression genes showed that most o ...
DOI:
10.1016/j.procbio.2016.05.017

9. Protein misfolding and aggregation: Mechanism, factors and detection

Author:
Sumit Kumar Chaturvedi; Mohammad Khursheed Siddiqi; Parvez Alam; Rizwan Hasan Khan
Source:
Process biochemistry 2016 v.51 no.9 pp. 1183-1192
ISSN:
1359-5113
Subject:
Alzheimer disease; Parkinson disease; amyloid; amyloidosis; human diseases; noninsulin-dependent diabetes mellitus; prion diseases; protein folding
Abstract:
... Amyloidogenic diseases are characterised by the formation of amyloid aggregates inside or outside the cell. Amyloid-associated human diseases include Alzheimer’s disease, Parkinson’s disease, prion diseases and type II diabetes. Currently, these diseases are incurable; thus, detailed insight into the mechanism of amyloid formation, deposition and inhibition is required to develop treatment strateg ...
DOI:
10.1016/j.procbio.2016.05.015

10. Purification and identification of an angiotensin I-converting enzyme inhibitory peptide from cauliflower by-products protein hydrolysate

Author:
Yang Xu; Tao Bao; Wen Han; Wei Chen; Xiaodong Zheng; Jianxu Wang
Source:
Process biochemistry 2016 v.51 no.9 pp. 1299-1305
ISSN:
1359-5113
Subject:
byproducts; cauliflower; environmental protection; functional foods; hydrolysates; hypertension; inhibitory concentration 50; leaf protein; leaves; molecular weight; pancreatin; pepsin; peptidyl-dipeptidase A; protein hydrolysates; purification methods; stems; subtilisin
Abstract:
... Cauliflower by-products (stems and leaves) are rich in leaf protein. Making full use of cauliflower by-products can not only alleviate the shortage of protein resources but also be beneficial to the environmental protection. This study sought to prepare and purify an angiotensin I-converting enzyme (ACE) inhibitory peptide from cauliflower by-products protein hydrolysate. Our result showed that th ...
DOI:
10.1016/j.procbio.2016.05.023

11. Structural characterization and anti-hypoxia activity of an exopolysaccharide isolated from fermentation broth of Lachnum sp.

Author:
Zhanzhan Du; Shuai Zong; Maheen Mahwish Surhio; Ping Xu; Liu Yang; Ming Ye
Source:
Process biochemistry 2016 v.51 no.9 pp. 1290-1298
ISSN:
1359-5113
Subject:
Fourier transform infrared spectroscopy; Lachnum; culture media; decolorization; erythrocytes; ethanol; exopolysaccharides; gas chromatography-mass spectrometry; gel chromatography; glucose; hematologic tests; hemoglobin; hypoxia; mannose; mice; molecular weight; nuclear magnetic resonance spectroscopy; scanning electron microscopy; sodium nitrite
Abstract:
... A novel exopolysaccharide (LEP-1) was isolated from the fermented broth of Lachnum YM268 through ethanol precipitation, deproteinization, decolorization and purification. The physicochemical properties of LEP-1 were determined by high performance gel permeation chromatography (HPGPC), field-emission scanning electron microscopy (SEM), fourier transform infrared spectrometry (FT-IR), gas chromatogr ...
DOI:
10.1016/j.procbio.2016.06.002

12. Enhanced stability of l-lactate dehydrogenase through immobilization engineering

Author:
E. Jackson; F. López-Gallego; J.M. Guisan; L. Betancor
Source:
Process biochemistry 2016 v.51 no.9 pp. 1248-1255
ISSN:
1359-5113
Subject:
NAD (coenzyme); engineering; enzyme activity; ethanol; lactate dehydrogenase; lactic acid; muscles; pH; pyruvic acid; rabbits; temperature; trehalose
Abstract:
... l- lactate dehydrogenase (LDH) catalyzes the conversion of pyruvate to l-lactate using NADH as a cofactor. In this work, we have optimized the immobilization of LDH from rabbit muscle in glyoxyl-agarose to have an active and stable preparation which is able to synthesize l-lactic acid. Optimization of various parameters during immobilization allowed the preparation of an active and highly stable i ...
DOI:
10.1016/j.procbio.2016.06.001

13. Feasible acetic acid fermentations of alcoholic and sugary substrates in combined operation mode

Author:
Maria Gullo; Gabriele Zanichelli; Elena Verzelloni; Federico Lemmetti; Paolo Giudici
Source:
Process biochemistry 2016 v.51 no.9 pp. 1129-1139
ISSN:
1359-5113
Subject:
acetic acid; acetic acid bacteria; cellulose; ethanol; microbial culture; oxidation; process control; reducing sugars; starter cultures; submerged fermentation; sugar content; vinegars
Abstract:
... Starting from small-scale batch cultivations, acetic acid fermentations in static and submerged systems have been performed by a single acetic acid bacterial strain. To provide user-friendly selected starter cultures for industry, the versatility of these cultures in using different oxidation substrates under different conditions was assessed. In all cases, vinegars with the desired acetic acid, r ...
DOI:
10.1016/j.procbio.2016.05.018

14. Immobilized multienzymatic systems for catalysis of cascade reactions

Author:
Qingzhi Ji; Bochu Wang; Jun Tan; Liancai Zhu; Liuying Li
Source:
Process biochemistry 2016 v.51 no.9 pp. 1193-1203
ISSN:
1359-5113
Subject:
biocatalysts; immobilized enzymes; pH; biocatalysis; mass transfer; temperature; stereochemistry
Abstract:
... Cascade reactions catalyzed by immobilized multienzymatic systems are an emerging technology, making catalytic strategies more sophisticated and effective. Thus, immobilized multienzymatic systems that exploit the chemo-, regio-, and stereoselectivity of biocatalysts have been developed. Recently, a variety of bioinspired immobilized multienzymatic systems was fabricated. In this review, immobiliz ...
DOI:
10.1016/j.procbio.2016.06.004

15. Impact of trace element additives on anaerobic digestion of sewage sludge with in-situ carbon dioxide sequestration

Author:
Jessica L. Linville; Yanwen Shen; Robin P. Schoene; Maximilian Nguyen; Meltem Urgun-Demirtas; Seth W. Snyder
Source:
Process biochemistry 2016 v.51 no.9 pp. 1283-1289
ISSN:
1359-5113
Subject:
additives; anaerobic digestion; biogas; carbon dioxide; carbon sequestration; carbonation; cobalt; heat; iron; methane; methane production; models; natural gas; nickel; pipelines; response surface methodology; sewage sludge; temperature; wastewater treatment
Abstract:
... Anaerobic digestion (AD) of sludge at wastewater treatment plants can benefit from addition of essential trace metals such as iron, nickel and cobalt to increase biogas production for utilization in combined heat and power systems, fed into natural gas pipelines or as a vehicle fuel. This study evaluated the impact and benefits of Ni/Co and olivine addition to the digester at mesophilic temperatur ...
DOI:
10.1016/j.procbio.2016.06.003

16. Integration of ethanol removal using carbon nanotube (CNT)-mixed membrane and ethanol fermentation by self-flocculating yeast for antifouling ethanol recovery

Author:
Chuang Xue; Zi-Xuan Wang; Guang-Qing Du; Li-Hai Fan; Ying Mu; Jian-Gang Ren; Feng-Wu Bai
Source:
Process biochemistry 2016 v.51 no.9 pp. 1140-1146
ISSN:
1359-5113
Subject:
adsorption; batch fermentation; bioethanol; bioreactors; carbon nanotubes; ethanol; ethanol fermentation; ethanol production; fouling; pervaporation; production costs; risk; yeasts
Abstract:
... Bioethanol is a renewable biofuel that has a strong inhibitory effect on cells in bioethanol fermentation by yeast. In this study, carbon nanotube (CNT)-mixed polydimethylsiloxane (PDMS) membranes were used for ethanol recovery from model solutions as well as for fermentation by self-flocculating yeast. Imbedding CNTs into the PDMS membrane led to enhanced ethanol recovery, with a maximum total fl ...
DOI:
10.1016/j.procbio.2016.05.030

17. Moderate pulsed electric field treatment enhances exopolysaccharide production by Lactococcus lactis subspecies cremoris

Author:
Tetsuro Ohba; Kunihiko Uemura; Hiroshi Nabetani
Source:
Process biochemistry 2016 v.51 no.9 pp. 1120-1128
ISSN:
1359-5113
Subject:
Lactococcus lactis; beta-galactosidase; biosynthesis; chemical composition; electric field; engineering; exopolysaccharides; fermentation; gel chromatography; molecular weight; permeability; pulsed electric fields
Abstract:
... An exopolysaccharide (EPS) producing strain of Lactococcus lactis subspecies cremoris was treated by pulsed electric field (PEF) while the strain was growing on the exponential phase and actively producing the EPS, in order to study how PEF treatment impacts on the biosynthesis of EPS. If a pulse with electric field of 8kV/cm and pulse-length of 1μs was shot for 200 times by one-pass treatment, th ...
DOI:
10.1016/j.procbio.2016.05.027

18. Multiphase mixing characteristics in a microcarrier-based stirred tank bioreactor suitable for human mesenchymal stem cell expansion

Author:
Tanja A. Grein; Jasmin Leber; Miriam Blumenstock; Florian Petry; Tobias Weidner; Denise Salzig; Peter Czermak
Source:
Process biochemistry 2016 v.51 no.9 pp. 1109-1119
ISSN:
1359-5113
Subject:
aeration; agitation; bioreactors; homogenization; humans; mixing; shear stress; stem cells
Abstract:
... Large-scale human mesenchymal stem cell expansion calls for a bioreaction system, that provides a sufficient growth surface. An alternative to static cultivations systems like cell factories are disposable stirred tank reactors. Here, microcarriers provide the required growth surface, but these make it difficult to achieve a complete homogenization in the bioreactor, while avoiding shear stress. T ...
DOI:
10.1016/j.procbio.2016.05.010

19. Production of silver nanoparticles using yeasts and evaluation of their antifungal activity against phytopathogenic fungi

Author:
Jorge G. Fernández; Martín A. Fernández-Baldo; Elias Berni; Gerardo Camí; Nelson Durán; Julio Raba; María I. Sanz
Source:
Process biochemistry 2016 v.51 no.9 pp. 1306-1313
ISSN:
1359-5113
Subject:
Cryptococcus laurentii; Fourier transform infrared spectroscopy; Rhodotorula glutinis; X-ray diffraction; antifungal properties; iprodione; nanoparticles; nanosilver; nitrate reductase; photons; plant pathogenic fungi; pome fruits; postharvest diseases; synthesis; transmission electron microscopy; ultraviolet-visible spectroscopy; yeasts; zeta potential
Abstract:
... The present study investigated the biological synthesis, characterization and antifungal activity of silver nanoparticles (Ag NPs). The production of Ag NPs was performed using the culture supernatants of two yeasts: Cryptococcus laurentii and Rhodotorula glutinis. These yeasts were chosen for their nitrate reductase activity. The role of nitrate reductase in the production of nanoparticles was as ...
DOI:
10.1016/j.procbio.2016.05.021

20. Relationship between p22phox expression, tag position and oxidase activity of the heterologous NADPH oxidase expressed in Pichia pastoris

Author:
Aymen Ezzine; Hager Souabni; Paul Machillot; Tania Bizouarn; Laura Baciou
Source:
Process biochemistry 2016 v.51 no.9 pp. 1239-1247
ISSN:
1359-5113
Subject:
NAD(P)H oxidase (H2O2-forming); innate immunity; Komagataella pastoris; yeasts; NADP (coenzyme); superoxide anion; membrane proteins; plasma membrane; toxicity; phagocytes; biomass
Abstract:
... The flavocytochrome b558 (Cytb558), heterodimer of two membrane proteins gp91ᵖʰᵒˣ and p22ᵖʰᵒˣ, corresponds to the catalytic part of the phagocyte NADPH-oxidase. This complex plays a crucial role of the innate immune system by generating superoxide anion, the precursor of toxic reactive oxygen species. Cytb558 lacks of precise structural knowledge due to an inefficient production of pure protein. A ...
DOI:
10.1016/j.procbio.2016.05.028