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You searched for: Journal Process biochemistry Remove constraint Journal: Process biochemistry Source 2019 v.79 Remove constraint Source: 2019 v.79 Subject biocatalysts Remove constraint Subject: biocatalysts
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1. Overexpression of epoxide hydrolase in Rhodococcus ruber with high robustness for the synthesis of chiral epichlorohydrin

Author:
Liang, Youxiang; Jiao, Song; Wang, Miaomiao; Yu, Huimin; Shen, Zhongyao
Source:
Process biochemistry 2019 v.79 pp. 49-56
ISSN:
1359-5113
Subject:
Agrobacterium radiobacter; Escherichia coli; Rhodococcus ruber; biocatalysts; enantiomers; epichlorohydrins; epoxide hydrolase; epoxides; glycols; pH; proteins; thermal stability
Abstract:
... Epoxide hydrolases (EHs) are attractive enzymes for producing enantiopure epoxides and diols, but do not display enough stability when lysates or Escherichia coli whole cells are used as biocatalysts. In this work, an organic-solvent tolerant strain Rhodococcus ruber THdAdN was utilized to overexpress an epoxide hydrolase from Agrobacterium radiobacter (ArEH), using E. coli BL21(DE3) as a control. ...
DOI:
10.1016/j.procbio.2018.12.023

2. Preparation of chiral β-hydroxytriazoles in one-pot chemoenzymatic bioprocesses catalyzed by Rhodotorula mucilaginosa

Author:
Aguirre-Pranzoni, Celeste; Tosso, Rodrigo D.; Bisogno, Fabricio R.; Kurina-Sanz, Marcela; Orden, Alejandro A.
Source:
Process biochemistry 2019 v.79 pp. 114-117
ISSN:
1359-5113
Subject:
Rhodotorula rubra; alkynes; ambient temperature; beta adrenergic receptors; bioactive properties; biocatalysts; copper; copper sulfate; cycloaddition reactions; enantiomers; ketones; sodium azide; stereoselectivity; triazoles
Abstract:
... Chemoenzymatic strategies for the preparation of enantiopure β-hydroxytriazoles were designed. These and other related compounds are particularly relevant because of their antitubercular bioactivities and as β-adrenergic receptor blockers. The ability of Rhodotorula mucilaginosa LSL to stereoselectively reduce prochiral ketones coupled to copper(I)-catalyzed azide-alkyne cycloaddition was exploite ...
DOI:
10.1016/j.procbio.2018.12.011

3. Strategies for enriching erucic acid from Crambe abyssinica oil by improved Candida antarctica lipase A variants

Author:
Zorn, Katja; Oroz-Guinea, Isabel; Bornscheuer, Uwe T.
Source:
Process biochemistry 2019 v.79 pp. 65-73
ISSN:
1359-5113
Subject:
Crambe abyssinica; Pseudozyma antarctica; biocatalysts; carboxylic ester hydrolases; crambe seed oil; erucic acid; esterification; esters; hydrolysis; industry; mutants; protein engineering; temperature profiles
Abstract:
... Erucic acid (C22:1, Δ13Z) is an interesting building block for the oleochemical industry and is abundantly present in Crambe abyssinica seed oil (∼59% of C22:1). For the enrichment of fatty acids (FA), lipases can be employed and we have found in a previous protein engineering study suitable variants of lipase A from Moesziomyces antarcticus (CAL-A) for the enrichment of C22:1 from Crambe oil deri ...
DOI:
10.1016/j.procbio.2018.12.022