PubAg

Main content area

You searched for: Journal Process biochemistry Remove constraint Journal: Process biochemistry Source 2019 v.83 Remove constraint Source: 2019 v.83
Start Over

Search

23  Search Results

« Previous | 1 - 20 of 23 |
Number of results to display per page

Search Results

1. A simple and efficient preparative procedure for menaquinone-7 from Bacillus subtilis (natto) using two-stage extraction followed by microporous resins

Author:
Fang, Zhiwei; Wang, Li; Zhao, Genhai; Liu, Hui; Wei, Hongfei; Wang, Han; Ni, Wenfeng; Zheng, Zhiming; Wang, Peng
Source:
Process biochemistry 2019 v.83 pp. 183-188
ISSN:
1359-5113
Subject:
Bacillus subtilis var. natto; crystallization; dietary supplements; ethanol; fermentation; medicinal properties; menaquinones; natto; porous media; resins
Abstract:
... Meanquinone-7 (MK-7) can be produced by the fermentation of Bacillus subtilis (natto), which is known for its nutraceutical and pharmacological properties. This study focused on the separation and purification of meanquinone-7 from wet cells produced by Bacillus subtilis (natto) fermentation. Using ethanol to extract menaquinone-7 twice directly from the crude cells after fermentation produced a m ...
DOI:
10.1016/j.procbio.2019.05.008

2. A study on cellulase production from a mixture of lignocellulosic wastes

Author:
Kumar Ramamoorthy, Navnit; Sambavi, T.R.; Renganathan, Sahadevan
Source:
Process biochemistry 2019 v.83 pp. 148-158
ISSN:
1359-5113
Subject:
Trichoderma harzianum; ammonia; bioenergy industry; biomass production; cardboard; cellulose; cotton; endo-1,4-beta-glucanase; enzyme activity; fungi; glucosidases; lignocellulose; lignocellulosic wastes; mass transfer; oxygen; packaging; solid state fermentation; submerged fermentation; sugars; viscosity; xylanases
Abstract:
... The production of cellulase with enhanced enzyme activities has been a challenge for the biofuel industries. In this work, to prove the technical feasibility of using an alternate source of cellulose for cellulase production, a novel lignocellulosic mixture of waste surgical cotton and packaging cardboard, and a cellulolytic fungus Trichoderma harzanium ATCC® 20846™ were employed. After a 9 day So ...
DOI:
10.1016/j.procbio.2019.05.006

3. Characterization of a novel d-lyxose isomerase from Thermoflavimicrobium dichotomicum and its application for D-mannose production

Author:
Zhang, Wenli; Huang, Jiawei; Jia, Min; Guang, Cuie; Zhang, Tao; Mu, Wanmeng
Source:
Process biochemistry 2019 v.83 pp. 131-136
ISSN:
1359-5113
Subject:
Escherichia coli; binding sites; enzyme activity; fructose; gene overexpression; half life; isomerases; isomers; manganese; mannose; melting point; molecular cloning; pH; phosphates; substrate specificity
Abstract:
... d-Mannose is the aldose isomer of d-fructose and displays unique physiological functions and health applications. As a result, it has attracted increasing interest from the public. Because of its wide substrate specificity, d-lyxose isomerase (D-LI) has been applied to d-mannose bioproduction. In this article, the Thermoflavimicrobium dichotomicum D-LI encoding gene was cloned and overexpressed in ...
DOI:
10.1016/j.procbio.2019.05.007

4. Characterization of novel cyclic lipopeptides produced by Bacillus sp. SY27F

Author:
Zhou, Hong; Cong, Bailin; Tian, Yongqi; He, Yu; Yang, Huanghao
Source:
Process biochemistry 2019 v.83 pp. 206-213
ISSN:
1359-5113
Subject:
Bacillus (bacteria); Candida albicans; Escherichia coli; Staphylococcus aureus; antibacterial properties; cell lines; cytotoxicity; fatty acids; gas chromatography-mass spectrometry; inhibitory concentration 50; lipopeptides; liquid chromatography; neoplasm cells; nuclear magnetic resonance spectroscopy; Indian Ocean
Abstract:
... Three new cyclic lipopeptides (compounds 1–3) were isolated from Bacillus sp. SY27F, which collected from the deep-sea hydrothermal vent in the Indian Ocean. The planar structures of compounds 1–3 were characterized by LC–MS, GC–MS and NMR, whereas their absolute configurations were determined by modified Marfey’s analysis and optical rotation contrast. They shared the same heptapeptide chain (N-L ...
DOI:
10.1016/j.procbio.2019.04.015

5. Development of a streptokinase expression platform using the native signal sequence of the protein with internal repeats 1 (PIR1) in P. pastoris: Gene dosage optimization and cell retention strategies

Author:
Adivitiya,; Babbal,; Mohanty, Shilpa; Dagar, Vikas Kumar; Khasa, Yogender Pal
Source:
Process biochemistry 2019 v.83 pp. 64-76
ISSN:
1359-5113
Subject:
Komagataella pastoris; culture flasks; enzymes; gene dosage; heterologous gene expression; hosts; product quality; proteolysis; recycling; secretion; signal peptide; toxicity
Abstract:
... Streptokinase is a therapeutically important thrombolytic agent which is toxic to its heterologous expression hosts and also prone to proteolytic degradation. In the current study, its secretory expression was targeted in P. pastoris using the native protein with internal repeats 1 (PIR1) secretion signal where the optimization of streptokinase gene dosage resulted in an increase in the volumetric ...
DOI:
10.1016/j.procbio.2019.05.016

6. Effective and long-term continuous bio-hydrogen production by optimizing fixed-bed material in the bioreactor

Author:
Zhao, Chenyu; Zhang, Nan; Zheng, Hanying; Zhu, Qi; Utsumi, Motoo; Yang, Yingnan
Source:
Process biochemistry 2019 v.83 pp. 55-63
ISSN:
1359-5113
Subject:
anaerobic digestion; bioactive properties; bioreactors; butyrates; glucose; hydrogen; hydrogen production; microorganisms; nylon; pH stability; polyethylene; porosity; roughness; scanning electron microscopy
Abstract:
... Three fixed-bed materials including chlorinated polyethylene (CPE), loofah sponge (LS) and porous nylon (PN) were investigated for enhancing bio-hydrogen production in anaerobic digestion. In the batch operation, the bioreactor fixed with CPE achieved the highest hydrogen production of 676.4 mL/L (5 g-glucose/L). The SEM observation revealed that CPE bioreactor immobilized the highest density of m ...
DOI:
10.1016/j.procbio.2019.04.021

7. Effective strategy for improving sludge treatment rate and microbial mechanisms during chromium bioleaching of tannery sludge

Author:
Zeng, Jing; Li, Jie; Gou, Min; Xia, Zi-Yuan; Sun, Zhao-Yong; Tang, Yue-Qin
Source:
Process biochemistry 2019 v.83 pp. 159-167
ISSN:
1359-5113
Subject:
Acidiphilium; Acidithiobacillus; Alicyclobacillus; Mycobacterium; Sulfobacillus; bioleaching; chromium; microbial communities; sludge; tannery waste
Abstract:
... By using an enriched microbial community, repeated-batch processes for bioleaching of chromium from tannery sludge was set up. The effects of batch-feeding ratio (FR) and sludge total solid (TS) on sludge retention time and treatment rate were investigated and a two-stage repeated-batch process was developed. The results of flask-scale repeated-batch bioleaching suggested that the sludge TS and FR ...
DOI:
10.1016/j.procbio.2019.05.019

8. Effects of different extraction techniques on physicochemical properties and biological activities of polysaccharides from Fritillaria pallidiflora Schrenk

Author:
Abuduwaili, Aytursun; Rozi, Parhat; Mutailifu, Paiheerding; Gao, Yanhua; Nuerxiati, Rehebati; Aisa, Haji Akber; Yili, Abulimiti
Source:
Process biochemistry 2019 v.83 pp. 189-197
ISSN:
1359-5113
Subject:
Fourier transform infrared spectroscopy; Fritillaria pallidiflora; X-ray diffraction; antioxidant activity; arabinose; chemical composition; galactose; glucose; hydroxyl radicals; mannose; moieties; oils; polysaccharides; rhamnose; scanning electron microscopy; solubility; ultrasonic treatment; uronic acids; xylose
Abstract:
... In this study, polysaccharides were extracted using different techniques including ultrasound-assisted extraction (UA), cellulase-assisted extraction (CA), cellulase-ultrasound-assisted extraction (CUA) and pectinase-ultrasound-assisted extraction (PUA) from Fritillaria pallidiflora Schrenk. Extraction yields, chemical composition, physicochemical, structural properties and antioxidant activities ...
DOI:
10.1016/j.procbio.2019.05.020

9. Electrochemical investigation of the inhibition effect of carvacrol on xanthine oxidase activity merging with theoretical studies

Author:
Rezaeinasab, Masoud; Benvidi, Ali; Gharaghani, Sajjad; Abbasi, Saleheh; Zare, Hamid R.
Source:
Process biochemistry 2019 v.83 pp. 86-95
ISSN:
1359-5113
Subject:
active sites; adjuvants; amino acids; carvacrol; dose response; electrochemistry; enzyme activity; gout; hydrogen bonding; hyperuricemia; least squares; medicinal properties; metabolism; uric acid; xanthine oxidase
Abstract:
... One of the key enzymes in purine catabolism is xanthine oxidase (XO), which is widely distributed in human limbs. Due to the excessive activity of XO in the body, hyperuricemia occurs as one of the consequences of uric acid overproduction, which finally leads to gout. In this study, the inhibitory effect of carvacrol on the XO activity was investigated by differential pulse voltammetry (DPV). The ...
DOI:
10.1016/j.procbio.2019.03.014

10. Fermentation performance and mechanism of a novel microbial consortium DUT08 for 1,3-propandiol production from biodiesel-derived crude glycerol under non-strictly anaerobic conditions

Author:
Sun, Yaqin; Zheng, Yafeng; Wang, Xiaoli; Zhou, Jinjie; Xiu, Zhilong
Source:
Process biochemistry 2019 v.83 pp. 27-34
ISSN:
1359-5113
Subject:
Clostridium butyricum; Escherichia coli; Klebsiella pneumoniae; anaerobic conditions; batch fermentation; glycerol; nitrogen; nucleotide sequences; oxygen; ribosomal RNA; symbiosis; wastewater
Abstract:
... In this study, a novel consortia DUT08 isolated from wastewater samples could efficiently covert crude glycerol to 1,3-propanediol (1,3-PDO) under anaerobic and microaerobic conditions. The physiological and biochemical characteristics of DUT08 indicated that it consisted of Clostridium (85.25%), Escherichia (12.54%), and Klebsiella (0.007%) with the neighbor-joining method of 16S rRNA gene sequen ...
DOI:
10.1016/j.procbio.2019.05.017

11. Hierarchically structured Fe3O4-doped MnO2 microspheres as an enhanced peroxidase-like catalyst for low limit of detection

Author:
Wang, Jianzhi; Huang, Fei; Wang, Xianming; Wan, Yinjia; Xue, Yanan; Cai, Ning; Chen, Weimin; Yu, Faquan
Source:
Process biochemistry 2019 v.83 pp. 35-43
ISSN:
1359-5113
Subject:
X-ray diffraction; catalysts; catalytic activity; detection limit; enzyme kinetics; glucose; hot water treatment; hydrogen peroxide; iron oxides; manganese dioxide; microparticles; nanoparticles; synergism; transmission electron microscopy
Abstract:
... In order to lower the limit of detection of glucose, a peroxidase-like artificial enzyme with elevated catalysis capacity has been achieved. Fe3O4-doped MnO2 microspheres were fabricated through a two-step hydrothermal method for this purpose. TEM revealed that down-sized Fe3O4 nanoparticles were dispersed throughout the urchin-like MnO2 burrs. An additional XRD peak beyond Fe3O4 and MnO2 nanopart ...
DOI:
10.1016/j.procbio.2019.05.014

12. Impact of fermentation conditions on the production of bioactive compounds with anticancer, anti-inflammatory and antioxidant properties in kombucha tea extracts

Author:
Villarreal-Soto, Silvia Alejandra; Beaufort, Sandra; Bouajila, Jalloul; Souchard, Jean-Pierre; Renard, Thierry; Rollan, Serge; Taillandier, Patricia
Source:
Process biochemistry 2019 v.83 pp. 44-54
ISSN:
1359-5113
Subject:
anti-inflammatory activity; antineoplastic activity; antioxidant activity; antioxidants; bioactive compounds; black tea; colorectal neoplasms; ethyl acetate; fermentation; gas chromatography-mass spectrometry; geometry; human cell lines; humans; inhibitory concentration 50; kombucha; neoplasm cells; organic acids and salts; pH; phenolic compounds; solvents
Abstract:
... The production of natural bioactive compounds through the fermentation of plants has increased in recent years. The biological activities of the extracts obtained from the fermentation of black tea with the kombucha consortium were evaluated. To improve the productivity of these compounds two different vessel geometries were used and successive extractions with solvents of increasing polarity were ...
DOI:
10.1016/j.procbio.2019.05.004

13. Impact of thermal treatment and hydrolysis by Alcalase and Cynara cardunculus enzymes on the functional and nutritional value of Okara

Author:
Voss, Glenise B.; Osorio, Hugo; Valente, Luísa M.P.; Pintado, Manuela E.
Source:
Process biochemistry 2019 v.83 pp. 137-147
ISSN:
1359-5113
Subject:
Cynara cardunculus; antioxidant activity; antioxidants; autoclaving; blood pressure; dietary supplements; enzymatic hydrolysis; hydrolysates; hydrolysis; ingredients; inhibitory concentration 50; nutritive value; peptides; subtilisin
Abstract:
... Enzymatic hydrolysis of dried okara (autoclaved and non-autoclaved) with Alcalase (AL) and Cynara cardunculus extract (CY) was studied, assessing the impact of heat treatment and hydrolysis on potential antioxidant and antihypertensive activities of final hydrolysates. This study showed that the thermal treatment (sterilization at 121 °C, 1 atm and 15 min) facilitated the enzymatic access to subst ...
DOI:
10.1016/j.procbio.2019.05.010

14. Induced effect of Ca2+ on curvulamine synthesis by marine-derived fungus Curvularia sp. IFB-Z10 under submerged fermentation

Author:
Wei, Xingchen; Liu, Changqing; An, Faliang; Lu, Yanhua
Source:
Process biochemistry 2019 v.83 pp. 18-26
ISSN:
1359-5113
Subject:
Curvularia; acetylcholinesterase; alkaloids; bioreactors; biosynthesis; calcium; calcium channels; calcium chloride; culture flasks; enzyme inhibition; gene expression regulation; genes; marine fungi; pellets; secondary metabolites; submerged fermentation; transcription (genetics)
Abstract:
... Curvulamine is a novel framework alkaloid with prominent antibacterial and acetylcholinesterase inhibitory activities produced by marine fungus, Curvularia sp. IFB-Z10. However, the low yield severely restricts its further pharmaceutical researches and applications. The study was aimed to enhance curvulamine production based on CaCl2 addition strategy and further clarify the underlying regulation ...
DOI:
10.1016/j.procbio.2019.03.011

15. Integrated production and separation of biosurfactants

Author:
Dolman, Ben M.; Wang, Fuju; Winterburn, James B.
Source:
Process biochemistry 2019 v.83 pp. 1-8
ISSN:
1359-5113
Subject:
biosurfactants; commercialization; engineering; fermentation; foams; fractionation; gravity; production costs
Abstract:
... Environmentally friendly, microbially produced surfactants known as biosurfactants have recently seen an explosion in commercial activity and interest due to a reduction in the cost of production, though these costs still limit biosurfactant use in bulk applications. These high production costs are primarily the result of the typically low productivities of large scale biosurfactant production pro ...
DOI:
10.1016/j.procbio.2019.05.002

16. Modeling and optimization of lipase-catalyzed hydrolysis for production of (S)-2-phenylbutyric acid enhanced by hydroxyethyl-β-cyclodextrin

Author:
Zhang, Panliang; Cheng, Qing; Xu, Weifeng; Tang, Kewen
Source:
Process biochemistry 2019 v.83 pp. 96-103
ISSN:
1359-5113
Subject:
beta-cyclodextrin; carboxylic ester hydrolases; enantioselectivity; hydrolysis; models; pH; response surface methodology; screening; solubility; temperature
Abstract:
... An efficient reactive system was established to produce (S)-2-phenylbutyric acid (2-PBA) through the enzymatic enantioselective hydrolysis of 2-phenylbutyrate ester (2-PBAE) in aqueous medium. Lipase CALA from Canadian antarctica and hexyl 2-phenylbutyrate (2-PBAHE) were identified upon screening as the best enzyme and substrate, respectively. Adding hydroxyethyl-β-cyclodextrin (HE-β-CD) to improv ...
DOI:
10.1016/j.procbio.2019.05.011

17. Naringenin-loaded nano-structured lipid carrier fortifies oxaliplatin-dependent apoptosis in HT-29 cell line

Author:
Raeisi, Sina; Chavoshi, Hadi; Mohammadi, Maryam; Ghorbani, Marjan; Sabzichi, Mehdi; Ramezani, Fatemeh
Source:
Process biochemistry 2019 v.83 pp. 168-175
ISSN:
1359-5113
Subject:
4',6-diamidino-2-phenylindole; adjuvants; adverse effects; apoptosis; cell proliferation; colorectal neoplasms; cytotoxicity; dose response; drug therapy; flow cytometry; gene expression; graphs; human cell lines; inhibitory concentration 50; lipids; nanoparticles; naringenin; neoplasm cells; particle size; pro-apoptotic proteins; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; scanning electron microscopy; staining
Abstract:
... In the present study, we inspected the role of naringenin-loaded nanostructured lipid carriers (NLCs) in the induction of apoptosis in (HT-29) colon cancer cell in co-administration with oxaliplatin. The cytotoxicity behavior of nanoparticles was investigated by MTT assay and DAPI staining. To understand the molecular mechanism of formulation in induction program cell death, flow cytometric and Re ...
DOI:
10.1016/j.procbio.2019.05.013

18. Nephroprotective effects of Lachnum melanin against acute kidney injury induced by cisplatin in mice

Author:
Yang, Liuqing; Yang, Qinghua; Li, Jinglei; Hou, Gouhua; Chen, Tingting; Ye, Ming
Source:
Process biochemistry 2019 v.83 pp. 198-205
ISSN:
1359-5113
Subject:
Lachnum; Western blotting; acute kidney injury; apoptosis; blood serum; caspase-3; catalase; cisplatin; creatinine; cytotoxicity; gene expression; glutathione; glutathione peroxidase; inducible nitric oxide synthase; interleukin-1beta; kidneys; malondialdehyde; melanin; mice; mitogen-activated protein kinase; necrosis; neoplasms; nephroprotective effect; oxidative stress; platinum; prostaglandin synthase; protein synthesis; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; superoxide dismutase; transcription factor NF-kappa B; tumor necrosis factor-alpha; urea nitrogen; weight loss
Abstract:
... The purpose of the present research was to discuss renoprotective effects of intracellular melanin from Lachnum YM156 (LIM) on cisplatin-induced acute kidney injury (AKI) in mice and to explore its possible mechanisms. No cytotoxicity of LIM on the HSF and LO2 cells was found by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cisplatin treatment resulted in noticeable ...
DOI:
10.1016/j.procbio.2019.05.001

19. Production, purification and functional characterization of phospholipase C from Bacillus thuringiensis with high catalytic activity

Author:
Eddehech, Ahlem; Smichi, Nabil; Arhab, Yani; Noiriel, Alexandre; Abousalham, Abdelkarim; Gargouri, Youssef; Zarai, Zied
Source:
Process biochemistry 2019 v.83 pp. 122-130
ISSN:
1359-5113
Subject:
Bacillus thuringiensis; catalytic activity; degumming; emulsifiers; fats and oils industry; magnesium; molecular weight; pH; phosphatidylcholines; phosphatidylethanolamines; phosphatidylserines; phospholipase C; polyacrylamide gel electrophoresis; secretion; substrate specificity
Abstract:
... The isolated Tunisian strain of Bacillus thuringiensis was characterized by the secretion of a phospholipase C (PLC). The activity of the extracellular PLC from B. thuringiensis (PLCBt) was optimized and the enzyme was purified to homogeneity. In fact, the yield reached 50% and the apparent molecular mass was determined to be 28 kDa as analyzed by SDS-PAGE and the N-terminal sequence PLCBt was cha ...
DOI:
10.1016/j.procbio.2019.05.012

20. Purification and extreme thermostabilization of glucoamylase by zinc produce of novel fungus Gymnoascella citrina

Author:
Yasin, Muhammad Zahid; Rashid, Muhammad Hamid
Source:
Process biochemistry 2019 v.83 pp. 114-121
ISSN:
1359-5113
Subject:
EDTA (chelating agent); calcium; fungi; glucan 1,4-alpha-glucosidase; half life; molecular weight; pH; polyacrylamide gel electrophoresis; starch; temperature; thermodynamics; zinc
Abstract:
... Extreme thermostabilization of glucoamylase (GA) from novel fungus Gymnoascella citrina was achieved first time through metals binding by using high temperature-unfolding- refolding technique. Crude GA (16.7 U mg−1 protein) produced by G. citrina was purified to homogeneity by using FPLC purification system. The molecular mass on SDS-PAGE was 88 kDa; optimum pH 4.5 and temperature was 55 °C. Kinet ...
DOI:
10.1016/j.procbio.2019.04.012