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You searched for: Journal name Process biochemistry Remove constraint Journal name: Process biochemistry Subject ascorbic acid Remove constraint Subject: ascorbic acid
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1. The impact of Vitamins C, B1 and B6 supplementation on antioxidant enzyme activities, membrane total sialic acid and lipid peroxidation levels in Fusarium species

Author:
Kayali, H.A.; Tarhan, L.
Source:
Process biochemistry 2006 v.41 no.7 pp. 1608-1613
Subject:
ascorbic acid; thiamin; pyridoxine; vitamin supplements; superoxide dismutase; catalase; glutathione peroxidase; reactive oxygen species; lipid peroxidation; sialic acids
DOI:
10.1016/j.procbio.2006.03.012

2. Purification and characterization of alkaline-stable β-amylase in malted African finger millet (Eleusine coracana) seed

Author:
Ayodele O. Kolawole; Joshua O. Ajele; Ravi Sirdeshmukh
Source:
Process biochemistry 2011 v.46 no.11 pp. 2178-2186
ISSN:
1359-5113
Subject:
EDTA (chelating agent); Eleusine coracana; Gibbs free energy; alpha-cyclodextrin; aluminum; amino acid sequences; ammonium sulfate; amylopectin; amylose; anion exchange; ascorbic acid; beta-amylase; enzyme stability; fractionation; gel chromatography; gibberellic acid; heat; iodoacetic acid; isoelectric point; malting; mercury; millets; molecular weight; pH; polyacrylamide gel electrophoresis; potato starch; seeds; temperature; trehalose
Abstract:
... Alkaline-stable β-amylase (EC 3.2.1.2) was purified to apparent homogeneity from malted African finger millet (Eleusine coracana) seed by ammonium sulfate fractionation and anion exchange and affinity chromatographies. Gel filtration chromatography together with SDS-PAGE revealed that the enzyme is monomeric, with a molecular weight of 59.1kDa. It has a pI value of 5.2 and is optimally active at p ...
DOI:
10.1016/j.procbio.2011.08.021

3. Sapodilla seed coat as a multifunctional ingredient for cosmetic applications

Author:
Mayuree Kanlayavattanakul; Nattaya Lourith
Source:
Process biochemistry 2011 v.46 no.11 pp. 2215-2218
ISSN:
1359-5113
Subject:
antioxidants; ascorbic acid; chlorogenic acid; correlation; cosmetics; ethyl acetate; gallic acid; hexane; ingredients; lipid peroxidation; quercetin; sapodillas; testa
Abstract:
... Sapodilla seed coat was extracted into n-hexane, EtOAc, and aqueous fractions (0.041, 0.085, and 1.124% (w/w)). The EtOAc fraction was the most potent antioxidant (IC₅₀DPPH=2.387±0.023, and IC₅₀ABTS=4.468±0.043μg/ml), and was significantly (p<0.05) more potent than ascorbic acid (IC₅₀DPPH=3.130±0.039, and IC₅₀ABTS=6.288±0.098μg/ml) with lipid peroxidation, and tyrosinase inhibitory effects (IC₅₀=4 ...
DOI:
10.1016/j.procbio.2011.08.022

4. 2,5-Diketo-gluconic acid reductase from Corynebacterium glutamicum: Characterization of stability, catalytic properties and inhibition mechanism for use in vitamin C synthesis

Author:
Vanja Kaswurm; Claudia Pacher; Klaus Dieter Kulbe; Roland Ludwig
Source:
Process biochemistry 2012 v.47 no.12 pp. 2012-2019
ISSN:
1359-5113
Subject:
Corynebacterium glutamicum; Escherichia coli; NADP (coenzyme); ascorbic acid; batch fermentation; calcium; chlorides; gene overexpression; genes; ions; magnesium; pH; screening; temperature
Abstract:
... 2,5-Diketo-d-gluconic acid (2,5-DKG) reductase is an NADPH-dependent, monomeric aldo-keto reductase (AKR) which catalyzes the reduction of 2,5-DKG to 2-keto-l-gulonic acid (2-KLG) – the immediate precursor of vitamin C. The reaction catalyzed by 2,5-DKG reductase is attractive to bypass several chemical steps and produce vitamin C biocatalytically. In a screening of 22 bacterial strains, nine 2,5- ...
DOI:
10.1016/j.procbio.2012.07.014

5. Ameliorative effect of 20-OH ecdysone on streptozotocin induced oxidative stress and β-cell damage in experimental hyperglycemic rats

Author:
Ramalingam Sundaram; Rajendran Naresh; Palanivelu Shanthi; Panchanatham Sachdanandam
Source:
Process biochemistry 2012 v.47 no.12 pp. 2072-2080
ISSN:
1359-5113
Subject:
alanine transaminase; animal disease models; antioxidant activity; antioxidants; ascorbic acid; aspartate transaminase; blood glucose; catalase; citrates; creatinine; diabetes mellitus; ecdysone; glibenclamide; glutathione; glutathione peroxidase; glutathione transferase; hyperglycemia; hypoglycemic agents; insulin; intraperitoneal injection; islets of Langerhans; kidneys; liver; oxidative stress; pH; rats; streptozotocin; superoxide dismutase; tissues; urea; vitamin E
Abstract:
... The present study was to evaluate the effects of 20-OH ecdysone on hyperglycemia mediated oxidative stress in streptozotocin induced diabetic rats. Diabetes was induced in experimental rats by single intraperitoneal injection of STZ (45mg/kg b.w.) dissolved in 0.1mol/L citrate buffer (pH 4.5). Diabetic rats exhibited increased blood glucose with significant decrease in plasma insulin levels. The a ...
DOI:
10.1016/j.procbio.2012.07.025

6. Purification, characterization and secondary structure elucidation of a detergent stable, halotolerant, thermoalkaline protease from Bacillus cereus SIU1

Author:
Sanjay Kumar Singh; Santosh Kumar Singh; Vinayak Ram Tripathi; Satyendra Kumar Garg
Source:
Process biochemistry 2012 v.47 no.10 pp. 1479-1487
ISSN:
1359-5113
Subject:
Bacillus cereus; acetic acid; ascorbic acid; beta-mercaptoethanol; blood; calcium; casein; chromatography; copper; cysteine; detergents; enzyme activity; ethylene; hydrogen peroxide; laundry; magnesium; manganese; metal ions; molecular weight; octoxynol; pH; polysorbates; proteinases; salt tolerance; sodium; sodium chloride; sodium dodecyl sulfate; temperature
Abstract:
... A thermoalkaline protease with a molecular weight of 22kDa was purified from the Bacillus cereus SIU1 strain using a combination of Q-Sepharose and Sephadex G-75 chromatography. The kinetic analyses revealed the Kₘ, Vₘₐₓ and kcₐₜ to be 1.09mgml⁻¹, 0.909mgml⁻¹min⁻¹ and 3.11s⁻¹, respectively, towards a casein substrate. The protease was most active and stable at pH 9.0 and between a temperature rang ...
DOI:
10.1016/j.procbio.2012.05.021

7. Synergism of an auxiliary activity 9 (AA9) from Chaetomium globosum with xylanase on the hydrolysis of xylan and lignocellulose

Author:
In Jung Kim; Hak Jin Youn; Kyoung Heon Kim
Source:
Process biochemistry 2016 v.51 no.10 pp. 1445-1451
ISSN:
1359-5113
Subject:
Chaetomium globosum; Escherichia coli; ascorbic acid; cellulose; endo-1,4-beta-glucanase; fungi; hydrolysis; lignocellulose; proteins; reducing sugars; rice straw; synergism; xylan; xylanases
Abstract:
... Auxiliary activity family 9 (AA9) is a group of fungal synergistic proteins belonging to the lytic polysaccharide monooxygenases. Via oxidative cleavage, AA9 facilitates cellulose hydrolysis by cellulase. However, oxidative activity of AA9 on pure xylan has not been reported yet. Previously, an AA9 from Chaetomium globosum (CgAA9) was identified and expressed in Escherichia coli. The recombinant C ...
DOI:
10.1016/j.procbio.2016.06.017

8. Characterization, purification, and temperature/pressure stability of polyphenol oxidase extracted from plums (Prunus domestica)

Author:
Elena Ioniţă; Leontina Gurgu; Iuliana Aprodu; Nicoleta Stănciuc; Istvan Dalmadi; Gabriela Bahrim; Gabriela Râpeanu
Source:
Process biochemistry 2017 v.56 pp. 177-185
ISSN:
1359-5113
Subject:
L-dopa; Prunus domestica; activation energy; ammonium sulfate; ascorbic acid; caffeic acid; catechin; catechol; catechol oxidase; chlorogenic acid; cysteine; enzyme activation; enzyme activity; ferulic acid; heat inactivation; hydrophobic interaction chromatography; ion exchange chromatography; kinetics; molecular weight; p-coumaric acid; p-cresol; pH; plums; polyacrylamide gel electrophoresis; temperature; tyrosine
Abstract:
... In this study, polyphenol oxidase (PPO) was extracted from Prunus domestica and partially purified by ammonium sulfate precipitation, hydrophobic interaction chromatography, and ion exchange chromatography. The final purification step revealed a 32.81-fold purification, and the molecular mass was estimated to be 65kDa by SDS-PAGE. The purified PPO showed enzymatic activity mainly toward five subst ...
DOI:
10.1016/j.procbio.2017.02.014

9. Fermentation-based biotransformation of bioactive phenolics and volatile compounds from cashew apple juice by select lactic acid bacteria

Author:
Ratchadaporn Kaprasob; Orapin Kerdchoechuen; Natta Laohakunjit; Dipayan Sarkar; Kalidas Shetty
Source:
Process biochemistry 2017 v.59 pp. 141-149
ISSN:
1359-5113
Subject:
2,2-diphenyl-1-picrylhydrazyl; Lactobacillus plantarum; antioxidant activity; ascorbic acid; biotransformation; byproducts; cashew nuts; fermentation; flavor; humans; lactic acid bacteria; metabolites; odors; plant health; plate count; proanthocyanidins; tannins; volatile compounds; whisky
Abstract:
... Select lactic acid bacteria (LAB); Lactobacillus plantarum, L. casei and L. acidophilus were targeted for enhancing bioactives and flavor volatiles of cashew apple juice (CAJ) that is an underutilized byproduct from cashew nut processing in Tropical countries. Results indicated the vitamin C and phenolic metabolites such as condensed tannin can be increased at certain stages such as at 12h over th ...
DOI:
10.1016/j.procbio.2017.05.019

10. Phytochemical composition and biological activities of Asteriscus graveolens (Forssk) extracts

Author:
Farah Ramdane; Rym Essid; Khaoula Mkadmini; Majdi Hammami; Nadia Fares; Mahfoud Hadj Mahammed; Dahmane El Ouassis; Olfa Tabbene; Ferid Limam; Mohamed Didi Ould Hadj
Source:
Process biochemistry 2017 v.56 pp. 186-192
ISSN:
1359-5113
Subject:
2,2-diphenyl-1-picrylhydrazyl; Leishmania; Listeria monocytogenes; aerial parts; antibacterial properties; antioxidant activity; antiparasitic properties; ascorbic acid; bacteria; bioactive compounds; coumaric acids; cytotoxicity; ethyl acetate; flavonoids; high performance liquid chromatography; inhibitory concentration 50; macrophages; parasites; pharmaceutical industry; polyphenols; tannins
Abstract:
... In the present study the phytochemical composition and biological activities of the aerial part extracts of Asteriscus graveolens against pathogenic bacteria and leishmania parasite were evaluated. Phytochemical analysis revealed the presence of polyphenols, flavonoids and tannins. The ethyl acetate fraction exhibited high antioxidant potential of 359.72±32.03μg and 326.76±15.86μg of ascorbic acid ...
DOI:
10.1016/j.procbio.2017.03.004

11. The effect of medium composition on deposition of collagen type 1 and expression of osteogenic genes in mesenchymal stem cells derived from human adipose tissue and bone marrow

Author:
Krisztina Szöke; Jana Daňková; Matej Buzgo; Evžen Amler; Jan E. Brinchmann; Esben Østrup
Source:
Process biochemistry 2017 v.59 pp. 321-328
ISSN:
1359-5113
Subject:
adipose tissue; ascorbic acid; bone formation; bone marrow; collagen; dexamethasone; genes; humans; nanofibers; stem cells; tissue engineering
Abstract:
... The two mesenchymal stem cell (MSC) populations that have gained most attention in relation to bone tissue engineering are adipose tissue (AT) MSCs and bone marrow (BM) MSCs. The purpose of this study was to investigate the ability of human BM-MSCs and AT-MSCs to survive, proliferate and deposit collagen type 1 when cultured on polycaprolactone nanofiber scaffolds and to ascertain the effect of me ...
DOI:
10.1016/j.procbio.2016.10.011

12. Highly efficient biosynthesis of α-arbutin from hydroquinone by an amylosucrase from Cellulomonas carboniz

Author:
Shuhuai Yu; Yongchun Wang; Yuqing Tian; Wei Xu; Yuxiang Bai; Tao Zhang; Wanmeng Mu
Source:
Process biochemistry 2018 v.68 pp. 93-99
ISSN:
1359-5113
Subject:
Cellulomonas; ascorbic acid; biosynthesis; enzymes; hydroquinone; industrialization; oxidation; pH; quinones; raw materials; sucrose; temperature
Abstract:
... Few reports about the biosynthesis of α-arbutin using amylosucrase are available. In this work, an amylosucrase from Cellulomonas carboniz T26, CcAS, was identified for highly efficient synthesis of α-arbutin using 20 mM sucrose and 5 mM hydroquinone as a glucosyl donor and acceptor, respectively. The optimum conditions for transglucosylation are a pH of 7.0 and a temperature of 40 °C. Under these ...
DOI:
10.1016/j.procbio.2018.02.012

13. Ecofriendly extraction of bioactive fractions from Sargassum muticum

Author:
María P. Casas; Enma Conde; Herminia Domínguez; Andrés Moure
Source:
Process biochemistry 2019 v.79 pp. 166-173
ISSN:
1359-5113
Subject:
Sargassum muticum; algae; alginates; antioxidant activity; ascorbic acid; cytotoxicity; enzymatic treatment; enzyme activity; enzymes; feedstocks; fibroblasts; humans; melanoma; mice; solubilization; ultrasonics; unsaturated fatty acids
Abstract:
... An ecofriendly process based on an enzyme-assisted extraction coupled with ultrasound extraction was proposed to obtain soluble bioactive fractions from Sargassum muticum. Whole algae (Sm) and residual algae from alginate production (AESm) were employed as feedstock. Aqueous enzyme treatment with commercial complex enzyme formulations or with mixture of enzymes lead to maximum solubilization yield ...
DOI:
10.1016/j.procbio.2018.12.021

14. Expression and enzymatic characterization of rice α-galactosidase II displayed on yeast cell surface

Author:
Mosi Dong; Tuoping Li; Suhong Li; Jia Guo; Yun Gong; Xin Qi
Source:
Process biochemistry 2019 v.81 pp. 57-62
ISSN:
1359-5113
Subject:
EDTA (chelating agent); Oryza sativa; Saccharomyces cerevisiae; alpha-galactosidase; arginine; ascorbic acid; guar gum; iron; pH; rice; soybean meal; storage quality; temperature; urea; yeasts
Abstract:
... A yeast (Saccharomyces cerevisiae EBY100) surface displayed rice α-galactosidase II (YSD rice α-Gal II) was constructed. The optimum temperature and pH of YSD rice α-Gal II were 45 °C and 4.0–5.5, respectively. Ascorbic acid significantly increased the expression level of YSD rice α-Gal II by 5.7-fold, compared to control, indicating its importance in the production and application of YSD rice α-G ...
DOI:
10.1016/j.procbio.2019.03.016

15. Metabolite profiling, molecular docking and in vitro anticancer potential of marine ascidian Didemnum sp.

Author:
Velusamy Arumugam; Manigandan Venkatesan; Selvakumar Murugesan; Rathinam Ayyasamy; Saravanan Ramachandran; Umamaheswari Sundaresan; Arivalagan Pugazhendhi
Source:
Process biochemistry 2019 pp. -
ISSN:
1359-5113
Subject:
Ascidiacea; Fourier transform infrared spectroscopy; aldehydes; alkanes; alkynes; amines; ascorbic acid; bioactive compounds; cell lines; computer simulation; cytotoxicity; epidermal growth factor receptors; ethanol; medicinal properties; metabolites; methanol; moieties; mouth neoplasms; oleic acid; organic halogen compounds; phenols
Abstract:
... Marine ascidians contain potential bioactive compounds, which exhibit significant pharmacological activities. In the present study, the MeOH extract of Didemnum sp. was subjected to FT-IR, GCMS analysis, the possible interactions with EGFR through docking analysis and in vitro cytotoxic activity against KB (Human oral cancer) cell line were also studied. The FT-IR analysis of Didemnum sp. revealed ...
DOI:
10.1016/j.procbio.2019.09.021

16. Synthesis of Oldenlandia umbellata stabilized silver nanoparticles and their antioxidant effect, antibacterial activity, and bio-compatibility using human lung fibroblast cell line WI-38

Author:
Palanisamy Subramanian; Anjali Ravichandran; Vinosha Manoharan; Reka Muthukaruppan; Selvakumar Somasundaram; Boomi Pandi; Anand Krishnan; Prabhu Narayanasamy Marimuthu; Selliah Swamy Nathan Somasundaram; SangGuan You
Source:
Process biochemistry 2019 v.86 pp. 196-204
ISSN:
1359-5113
Subject:
Escherichia coli; Fourier transform infrared spectroscopy; Oldenlandia; Streptococcus mutans; antibacterial properties; antioxidant activity; antioxidants; ascorbic acid; biocompatibility; cell viability; chemical constituents of plants; dose response; fibroblasts; human cell lines; lungs; nanoparticles; nanosilver; scanning electron microscopes; screening; therapeutics; toxicity; transmission electron microscopes; ultraviolet-visible spectroscopy
Abstract:
... In the past few years, green synthesized silver nanoparticles (AgNPs) are one of predominantly used materials in therapeutics and clinical practices. In this study, AgNPs were synthesized from aqueous extract of Oldenlandia umbellata and investigated their pharmaceutical properties. UV–vis spectroscopy of O. umbellata mediated silver nanoparticles (AgNPs@OUa) showed a peak at 423 nm. Fourier trans ...
DOI:
10.1016/j.procbio.2019.08.002

17. Enzyme-assisted extraction of a cup plant (Silphium perfoliatum L.) Polysaccharide and its antioxidant and hypoglycemic activities

Author:
Yang Guo; Hongmei Shang; Jiangchao Zhao; Hexiang Zhang; Shilun Chen
Source:
Process biochemistry 2020 v.92 pp. 17-28
ISSN:
1359-5113
Subject:
2,2-diphenyl-1-picrylhydrazyl; Silphium perfoliatum; acarbose; agarose; alpha-amylase; alpha-glucosidase; antioxidant activity; antioxidants; arabinose; ascorbic acid; cellulose; enzymatic hydrolysis; enzyme activity; galactose; galacturonic acid; glucose; glycemic effect; hypoglycemic agents; mannose; pH; response surface methodology
Abstract:
... The optimum enzyme-assisted extraction conditions of cup plant (Silphium perfoliatum L.) polysaccharides (CPP) were obtained by response surface methodology (RSM), and were found to be an enzymatic hydrolysis time of 66 min, a liquid-solid ratio of 22 mL/g, pH 5.1, and an enzyme-complex dosage of 1.59 %, allowing a CPP yield of 13.69 %. DEAE-52 cellulose and Sepharose CL-6B columns were used to pu ...
DOI:
10.1016/j.procbio.2020.03.005

18. Polyherbal drug loaded starch nanoparticles as promising drug delivery system: Antimicrobial, antibiofilm and neuroprotective studies

Author:
Prakashkumar Nallasamy; Thenmozhi Ramalingam; Thajuddin Nooruddin; Rajasree Shanmuganathan; Pugazhendhi Arivalagan; Suganthy Natarajan
Source:
Process biochemistry 2020 v.92 pp. 355-364
ISSN:
1359-5113
Subject:
Salmonella Typhi; Shigella dysenteriae; X-ray diffraction; acetylcholinesterase; antibacterial properties; antioxidant activity; ascorbic acid; biopolymers; drug delivery systems; drugs; encapsulation; enzyme inhibition; free radical scavengers; hormones; humans; light scattering; methicillin-resistant Staphylococcus aureus; nanoparticles; neuroprotective effect; pH; pharmaceutical industry; polyphenols; solubility; starch; ultraviolet-visible spectroscopy; zeta potential
Abstract:
... Starch is a natural, renewable biopolymer widely used in pharmaceutical industry for the controlled release of drugs and hormones. Triphala Churna (TC) a tridoshic rasayana, rich in polyphenols and vitamin C possess balancing and rejuvenating effect on three constitutional elements that govern the human life. To enhance the solubility and efficiency of the ayurvedic drug TC, attempts were made to ...
DOI:
10.1016/j.procbio.2020.01.026

19. High performance nanozymatic assay-based CuO nanocluster supported by reduced graphene oxide for determination of hydrogen peroxide and ascorbic acid

Author:
Fatemeh Honarasa; Rooza Mokhtare; Azadeh Mokhtare; Saeed Yousefinejad
Source:
Process biochemistry 2021 v.111 pp. 256-261
ISSN:
1359-5113
Subject:
absorbance; ascorbic acid; bovine serum albumin; catalytic activity; color; copper sulfate; detection limit; enzymes; graphene oxide; hydrogen peroxide; nanocomposites; oxidation; stabilizers; synergism; ultraviolet-visible spectroscopy
Abstract:
... Several efforts have been made to increase the nanozymatic activity of reduced Graphene Oxide (rGO). Also, the enzyme mimetic research with Nanoclusters (NCs) is in the embryo stage. For the first time, a one pot synthesis method was used successfully for preparation of CuONCs loaded r-GO (CuONCs/rGO). GO and copper sulfate were used as precursors and Bovine Serum Albumin (BSA) as the reducer and ...
DOI:
10.1016/j.procbio.2021.10.025

20. Highly efficient and low-cost process for synthesis of 2-O-α-d-glucopyranosyl-6-O-(2-propylpentanoyl)-l-ascorbic acid

Author:
Yuji Iwaoka; Misaki Fukushima; Hideyuki Ito; Akihiro Tai
Source:
Process biochemistry 2021 v.111 pp. 71-77
ISSN:
1359-5113
Subject:
Rhizopus; Thermoanaerobacter; acetates; acylation; ambient temperature; antineoplastic activity; antineoplastic agents; ascorbic acid; cyclomaltodextrin glucanotransferase; glycosylation; pH; starch
Abstract:
... 2-O-α-d-Glucopyranosyl-6-O-(2-propylpentanoyl)-l-ascorbic acid (6-bOcta-AA-2G) has shown a remarkable antitumor effect in an in vivo study. However, an efficient and low-cost process for synthesis of 6-bOcta-AA-2G has not been established yet. The process used for synthesis of 6-bOcta-AA-2G in this study consisted of three steps. The first step was acylation of l-ascorbic acid (AA) at the C-6 posi ...
DOI:
10.1016/j.procbio.2021.09.004