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You searched for: Journal name Process biochemistry Remove constraint Journal name: Process biochemistry Subject epoxides Remove constraint Subject: epoxides
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1. Development and application of metal chelate-epoxy bifunctional loofah sponge for the purification and immobilization of recombinant trehalose synthase

Author:
Jui-Chia Chang; Yi-An Chen; Sung-Chyr Lin
Source:
Process biochemistry 2022 v.116 pp. 108-115
ISSN:
1359-5113
Subject:
adsorption; enzyme activity; epoxides; immobilized enzymes; sorption isotherms; thermal stability; trehalose
Abstract:
... The preparation and application of metal chelate-epoxy bifunctional loofah sponge for the selective adsorption and covalent immobilization of a His-tagged trehalose synthase were reported. The adsorption of the His-tagged trehalose synthase on the metal chelate loofah sponge was found to follow the Langmuir isotherm model with Co²⁺ ion exhibiting an adsorption capacity of 1.183 mg/g and a superior ...
DOI:
10.1016/j.procbio.2022.03.011

2. Enantioconvergent hydrolysis of racemic epoxides at elevated concentrations in Tween-20/phosphate buffer by the corresponding epoxide hydrolases

Author:
Bochun Hu; Xiuxiu Zhu; Dong Zhang; Wenqian Lin; Mengdi Wu; Minchen Wu
Source:
Process biochemistry 2022 v.115 pp. 92-99
ISSN:
1359-5113
Subject:
epoxides; hydrolases; hydrolysis; pH; phosphates; styrene
Abstract:
... For effectively actualizing the gram-scale preparation of (R)-phenyl-1,2-ethanediol (PED) via the enantioconvergent hydrolysis of racemic (rac-) styrene oxide (SO) at elevated concentration using whole-cell of E. coli/sleh2, the reaction media were constructed by adding non-ionic surfactants, organic solvents, and deep eutectic solvents into phosphate buffer, respectively. After screening, using c ...
DOI:
10.1016/j.procbio.2022.02.007

3. Concomitant selective adsorption and covalent immobilization of a His-tagged protein switch with silica-based metal chelate-epoxy bifunctional adsorbents

Author:
Chen-Xin You; Po-Han Huang; Sung-Chyr Lin
Source:
Process biochemistry 2020 v.99 pp. 179-186
ISSN:
1359-5113
Subject:
adsorbents; adsorption; biosensors; chemical bonding; enzyme activity; epoxides; maltose; pH; silane
Abstract:
... A series of silica-based bifunctional adsorbents containing both metal-chelating groups and epoxy groups for the concomitant purification and immobilization of His-tagged protein switch RG13, a potential bioreceptor for developing maltose biosensors, were prepared by controlling the ratio of iminodiacetic acid-conjugated silane (GLYMO-IDA) and silane (GLYMO) used for surface modification. The bifu ...
DOI:
10.1016/j.procbio.2020.09.005

4. Optimizing Immobilization and Stabilization of Feruloyl Esterase from Humicola Insolens and its Application for the Feruloylation of Oligosaccharides

Author:
Juan Tamayo-Cabezas; Salwa Karboune
Source:
Process biochemistry 2020 v.98 pp. 11-20
ISSN:
1359-5113
Subject:
Humicola insolens; biocatalysts; biosynthesis; enzyme activity; epoxides; esterification; feruloyl esterase; pH; porosity; response surface methodology; thermal stability; xylooligosaccharides
Abstract:
... Feruloyl esterase (FAE)-catalyzed esterification reaction is as a potential route for the biosynthesis of feruloylated oligosaccharides as functional ingredients. Immobilization of FAE from Humicola insolens on metal chelate-epoxy supports was investigated. The study of effects of immobilization parameters using response surface methodology revealed the significance of enzyme/support ratio (3.25-2 ...
DOI:
10.1016/j.procbio.2020.07.009

5. Immobilized feruloyl esterase from Humicola insolens catalyzes the synthesis of feruloylated oligosaccharides

Author:
Juan Tamayo-Cabezas; Salwa Karboune
Source:
Process biochemistry 2019 v.79 pp. 81-90
ISSN:
1359-5113
Subject:
Humicola insolens; acylation; catalytic activity; emulsions; epoxides; ferulic acid; feruloyl esterase; magnetism; microparticles; nanoparticles; raffinose; substrate specificity
Abstract:
... Ferulic acid acylation of oligosaccharides catalyzed by feruloyl esterases (FAE) is a promising route to produce feruloylated oligosaccharides. However, modulation of FAE synthetic properties is a key step to improve the acylation. The efficiency of H. insolens FAE to catalyze the feruloylation in six different surfactantless microemulsions reaction systems was evaluated. The highest yield (57%) w ...
DOI:
10.1016/j.procbio.2018.12.013

6. Modulation of Lecitase properties via immobilization on differently activated Immobead-350: Stabilization and inversion of enantiospecificity

Author:
Maísa P. Pinheiro; Rodolpho R.C. Monteiro; Francisco F.M. Silva; Telma L.G. Lemos; Roberto Fernandez-Lafuente; Luciana R.B. Gonçalves; José C.S. dos Santos
Source:
Process biochemistry 2019 v.87 pp. 128-137
ISSN:
1359-5113
Subject:
acetates; acetonitrile; biocatalysts; butyrates; catalytic activity; enzymes; epoxides; hydrolysis; pH
Abstract:
... Lecitase Ultra (Leci) was immobilized on Immobead-350 (IB-350) activated with epoxy (EPO), amino-divinylsulfone (DVS) or amino-glutaraldehyde (GLU) groups. Leci-GLU had 27% more activity versus p-nitro-phenyl butyrate (p-NPB) than Leci-EPO, after 3 h of immobilization using 100 mg of enzyme/g of support (immobilization yield was 84.1%, expressed activity versus p-NPB was around 10%), the covalent ...
DOI:
10.1016/j.procbio.2019.08.016

7. Overexpression of epoxide hydrolase in Rhodococcus ruber with high robustness for the synthesis of chiral epichlorohydrin

Author:
Youxiang Liang; Song Jiao; Miaomiao Wang; Huimin Yu; Zhongyao Shen
Source:
Process biochemistry 2019 v.79 pp. 49-56
ISSN:
1359-5113
Subject:
Agrobacterium radiobacter; Escherichia coli; Rhodococcus ruber; biocatalysts; enantiomers; epichlorohydrins; epoxide hydrolase; epoxides; glycols; pH; proteins; thermal stability
Abstract:
... Epoxide hydrolases (EHs) are attractive enzymes for producing enantiopure epoxides and diols, but do not display enough stability when lysates or Escherichia coli whole cells are used as biocatalysts. In this work, an organic-solvent tolerant strain Rhodococcus ruber THdAdN was utilized to overexpress an epoxide hydrolase from Agrobacterium radiobacter (ArEH), using E. coli BL21(DE3) as a control. ...
DOI:
10.1016/j.procbio.2018.12.023

8. Heterologous overexpression of Pseudomonas umsongensis halohydrin dehalogenase in Escherichia coli and its application in epoxide asymmetric ring opening reactions

Author:
Feng Xue; Xiangju Ya; Qi Tong; Yuansong Xiu; He Huang
Source:
Process biochemistry 2018 v.75 pp. 139-145
ISSN:
1359-5113
Subject:
Escherichia coli; Lewis bases; Pseudomonas; bromides; catalytic activity; chlorides; enantioselectivity; enzymes; epoxides; gene overexpression; genes; nitrites; pH; sequence analysis; temperature; thiocyanates
Abstract:
... A new halohydrin dehalogenase-like gene (HHDH) in the genome of Pseudomonas umsongensis YCIT1612 was identified, cloned and over-expressed in Escherichia coli. HHDH-Pu exhibited the highest sequence identity of 45% in comparison with the previously reported HHDHs. The recombinant HHDH-Pu was purified to homogeneity and its catalytic characteristics in epoxide ring opening was explored. Compared to ...
DOI:
10.1016/j.procbio.2018.09.018

9. Immobilization of antimicrobial core-shell nanospheres onto silicone for prevention of Escherichia coli biofilm formation

Author:
Margarida M. Fernandes; Kristina Ivanova; Antonio Francesko; Ernest Mendoza; Tzanko Tzanov
Source:
Process biochemistry 2017 v.59 pp. 116-122
ISSN:
1359-5113
Subject:
Escherichia coli; antibiotics; bacteria; biofilm; biomass; biopolymers; catheters; chemistry; epoxides; immune system; morbidity; mortality; nanospheres; polystyrenes; silicone; urinary tract diseases
Abstract:
... Escherichia coli (E. coli) strains are among the most frequently isolated microorganisms in urinary tract infections able to colonize the surface of urinary catheters and form biofilms. These biofilms are highly resistant to antibiotics and host immune system, resulting in increased morbidity and mortality rates. Strategies to prevent biofilm development, especially via restricting the initial sta ...
DOI:
10.1016/j.procbio.2016.09.011

10. Improved catalytic properties of Candida antarctica lipase B multi-attached on tailor-made hydrophobic silica containing octyl and multifunctional amino- glutaraldehyde spacer arms

Author:
Vinicius Vescovi; Willian Kopp; José M. Guisán; Raquel L.C. Giordano; Adriano A. Mendes; Paulo W. Tardioli
Source:
Process biochemistry 2016 v.51 no.12 pp. 2055-2066
ISSN:
1359-5113
Subject:
Pseudozyma antarctica; adsorbents; alcohols; biocatalysts; carboxylic ester hydrolases; epoxides; fructose; glutaraldehyde; hydrophobicity; oleic acid; silica; temperature; thermal stability
Abstract:
... This work evaluated the immobilization of Candida antarctica lipase B (CALB) on a new heterofunctional support, prepared by silanization of silica particles with triethoxy(octyl)silane and trietoxy(3-aminopropyl)silane, followed by activation with glutaraldehyde. The new support (octyl-silica-amino-glutaraldehyde, OSGlu) exhibits features of an anionic exchanger and hydrophobic adsorbent capable o ...
DOI:
10.1016/j.procbio.2016.09.016

11. Optimization of the lipase mediated epoxidation of monoterpenes using the design of experiments—Taguchi method

Author:
Sumanth Ranganathan; Johannes Tebbe; Lars O. Wiemann; Volker Sieber
Source:
Process biochemistry 2016 v.51 no.10 pp. 1479-1485
ISSN:
1359-5113
Subject:
Candida; Taguchi method; alpha-pinene; carboxylic ester hydrolases; carene; chemical industry; epoxides; experimental design; hydrogen peroxide; industrial applications; limonene; purification methods; temperature
Abstract:
... This work deals with the optimization of the Candida antartica lipase B (CALB) mediated epoxidation of monoterpenes by using the design of experiments (DoE) working with the Taguchi Method. Epoxides are essential organic intermediates that find various industrial applications making the epoxidation one of the most investigated processes in chemical industry. As many as 8 parameters such as the rea ...
DOI:
10.1016/j.procbio.2016.07.005

12. Study on immobilization of (+) γ-lactamase using a new type of epoxy graphene oxide carrier

Author:
Wei Li; Huijiao Wen; Qianqiao Shi; Guojun Zheng
Source:
Process biochemistry 2016 v.51 no.2 pp. 270-276
ISSN:
1359-5113
Subject:
activation energy; enzyme activity; epoxides; graphene oxide; heat tolerance; immobilized enzymes; pH; temperature
Abstract:
... In this article, a new type of graphene oxide material was obtained by modification with epoxy chloropropane, which was used for the (+) γ-lactamase immobilization research. The enzymatic properties of the immobilized (+) γ-lactamase were systematically tested and compared with those of the free enzyme. The free and immobilized enzymes have the same optimum temperature (80°C) and pH (8.0), the ran ...
DOI:
10.1016/j.procbio.2015.11.030

13. Tailor-made design of penicillin G acylase surface enables its site-directed immobilization and stabilization onto commercial mono-functional epoxy supports

Author:
Valeria Grazu; Fernando López-Gallego; Jose Manuel Guisán
Source:
Process biochemistry 2012 v.47 no.12 pp. 2538-2541
ISSN:
1359-5113
Subject:
Escherichia coli; benzylpenicillin; epoxides; lysine; mutants; solvents; temperature
Abstract:
... Four different mutants of penicillin G acylase from E. coli (PGA) were site-direct immobilized and rigidification on Eupergit C. The resulting immobilized preparations were more stable against both temperature and organic solvents than those obtained by using a more complex tailor-made disulfide-epoxy supports. The region around the position β380 was the most interesting one to achieve the highest ...
DOI:
10.1016/j.procbio.2012.07.010