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You searched for: Journal name Process biochemistry Remove constraint Journal name: Process biochemistry Subject term catalytic activity Remove constraint Subject term: catalytic activity
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1. Applications of immobilized lipases in enzymatic reactors: A review

Author:
Daniela Remonatto; Rodney H. Miotti Jr.; Rubens Monti; Juliana C. Bassan; Ariela Veloso de Paula
Source:
Process biochemistry 2022 v.114 pp. 1-20
ISSN:
1359-5113
Subject:
carboxylic ester hydrolases; catalytic activity; half life; immobilized enzymes; mass transfer; temperature; viscosity
Abstract:
... Lipases are efficient biocatalysts with numerous applications in different industrial sectors, such as pharmaceutical, food, and fine chemistry industries. Enzyme immobilization further extends the applications of lipases by enhancing stability, selectivity, and half-life. However, obtaining high catalytic efficiency in reactions catalyzed by immobilized lipases requires optimization of reaction c ...
DOI:
10.1016/j.procbio.2022.01.004

2. Characterisation of biomass degrading xylanolytic enzymes of Penicillium chrysogenum produced using sugarcane bagasse

Author:
Sadia Fida Ullah; Amanda Araújo Souza; Sonia M. de Freitas; Eliane Ferreira Noronha
Source:
Process biochemistry 2022 v.112 pp. 62-70
ISSN:
1359-5113
Subject:
Penicillium chrysogenum; biomass; catalytic activity; cinnamic acid; fluorescence; gallic acid; histidine; ionization; mass spectrometry; oats; pH; sugarcane bagasse; tannins; thermal stability; vanillin; xylan; xylanases; xylose
Abstract:
... •Penicillium chrysogenum CCDCA10746 secretome (SPc) produced using raw sugarcane bagasse was used to hydrolyse the hydrothermally pretreated sugarcane bagasse, in order to solubilise xylan to produce xylose with the conversion rate of 67%. The secretome was active in a wide pH range and exhibits increased thermostability (t₁/₂ 8 h) than purified PcX2, a 23 kDa endo-β-1,4-xylanase of GH11 family, i ...
DOI:
10.1016/j.procbio.2021.11.012

3. Characterization of a sorbose oxidase involved in the biosynthesis of 2-keto-L-gulonic acid from Gluconobacter oxydans WSH-004

Author:
Yue Chen; Dong Li; Xiaoyu Shan; Jingwen Zhou; Jian Chen
Source:
Process biochemistry 2022 v.116 pp. 1-7
ISSN:
1359-5113
Subject:
Gluconobacter oxydans; NADP (coenzyme); ascorbic acid; biosynthesis; catalytic activity; fermentation; sorbose; sorbose dehydrogenase
Abstract:
... 2-Keto-L-gulonic acid (2-KLG), the direct precursor of vitamin C, can be synthesized from L-sorbose by many microorganisms via “sorbose dehydrogenase” and “sorbosone dehydrogenase (SNDH)”. However, only a few studies were carried out on the catalytic properties of sorbose dehydrogenase in Gluconobacter oxydans. In this study, sorbose dehydrogenase of G. oxydans WSH-004 was identified. Different fr ...
DOI:
10.1016/j.procbio.2022.02.019

4. Direct production of ethyl levulinate from carbohydrates and biomass waste catalyzed by modified porous silica with multiple acid sites

Author:
Luxin Zhang; Lu Tian; Ziyuan Xu; Lei Wang
Source:
Process biochemistry 2022 v.121 pp. 152-162
ISSN:
1359-5113
Subject:
biomass; byproducts; catalytic activity; cellobiose; cellulose; ethanolysis; filtration; glucose; kitchen waste; silica; surface area; synergism; wheat flour
Abstract:
... Three modified porous silica solid acid catalysts with multiple acid sites (SO₃H-Al/Silica, SO₃H-Al-Zr/Silica, and SO₃H-Zr/Silica) were prepared and characterized. The Pyridine-infrared spectra showed that both Brønsted (B) and Lewis (L) acid sites were present in all three catalysts. Catalytic acid sites with different acid strengths were also found, including strong sulfonic acids and several we ...
DOI:
10.1016/j.procbio.2022.06.036

5. Enhanced catalytic performance of thermophilic GH11 xylanase by fusing carbohydrate-binding module 9-2 and linker for better synergistic degradation of wheat bran

Author:
Yangyang Li; Weiyan Song; Xiaoqiang Yin; Shengqi Rao; Quan Zhang; Jingwen Zhou; Jianghua Li; Guocheng Du; Song Liu
Source:
Process biochemistry 2022 v.121 pp. 349-359
ISSN:
1359-5113
Subject:
active sites; carbohydrate binding; catalytic activity; endo-1,4-beta-glucanase; hydrogen; hydrolysis; lignocellulose; sugar content; sugarcane; weight loss; wheat bran; xylan; xylanases; xylose
Abstract:
... In this study, the catalytic activity of xynAm1 (a thermophilic xylanase family 11 variant) was significantly enhanced by fusing a carbohydrate-binding module (CBM) 9–2. First, CBM9–2 was fused to the N- and C-termini of xynAm1, generating the fusion enzymes C-X and X-C, respectively. The specific activity of C-X was 19.0 U/μM, which was 2.3-fold and 1.1-fold higher than that of xynAm1 and X-C, re ...
DOI:
10.1016/j.procbio.2022.07.021

6. Enhancing the catalytic activity of cyanobacterial chlorophyllase from Oscillatoria acuminata PCC 6304 through rational site-directed mutagenesis

Author:
Sitian Gu; Xiaojun Dai; Jiang Jiang; Yuanfa Liu
Source:
Process biochemistry 2022 v.114 pp. 111-118
ISSN:
1359-5113
Subject:
Chenopodium album; Chlamydomonas reinhardtii; Escherichia coli; Oscillatoria acuminata; Triticum aestivum; catalytic activity; chlorophyll; chlorophyllase; circular dichroism spectroscopy; heterologous gene expression; mutants; sequence alignment; site-directed mutagenesis; substrate specificity
Abstract:
... In our previous study, a novel cyanobacterial chlorophyllase from Oscillatoria acuminata (OaCLH) was successfully overexpressed and biochemically characterized in Escherichia coli BL21(DE3). Recombinant OaCLH has higher heterologous expression in E. coli than plant chlorophyllases. However, catalytic efficiency (kcₐₜ/Kₘ) of the recombinant OaCLH is significantly lower than those of several reporte ...
DOI:
10.1016/j.procbio.2022.01.023

7. Enzyme immobilization: Implementation of nanoparticles and an insight into polystyrene as the contemporary immobilization matrix

Author:
Shivam Sharma; Shubham Gupta; Princy; Shailendra Kumar Arya; Anupreet Kaur
Source:
Process biochemistry 2022 v.120 pp. 22-34
ISSN:
1359-5113
Subject:
beta-fructofuranosidase; catalytic activity; energy; enzyme activity; immobilized enzymes; laccase; magnetism; pH; polystyrenes; surface area; temperature; thermal stability
Abstract:
... Enzyme immobilization has plethora of applications in industrial and production activities. This is predominantly because immobilized enzymes have elevated long-term operational stability against pH, temperature, contaminants etc. Besides, these have extended shelf lives and are simpler to handle. Immobilized enzymes have enhanced activity, reclaimable as well as recoverable. Since the traditional ...
DOI:
10.1016/j.procbio.2022.05.022

8. High level production of γ-aminobutyric acid in engineered Escherichia coli by refactoring the glutamate decarboxylase

Author:
Jia-Fei Lyu; Chang-Jiang Lyu; Jia-Ren Cao; Jia-Qi Mei; Sheng Hu; Wei-Rui Zhao; Tian-Yi Xu; Yu-Ting Wang; Dan-Li Wang; Jun Huang; Le-He Mei
Source:
Process biochemistry 2022 v.118 pp. 243-251
ISSN:
1359-5113
Subject:
Escherichia coli; bioactive compounds; biocatalysts; catalytic activity; decarboxylation; gamma-aminobutyric acid; genes; glutamate decarboxylase; glutamic acid; mutants; pH; site-directed mutagenesis
Abstract:
... Gamma-Aminobutyric acid (GABA), an important bioactive compound, is synthesized through the decarboxylation of L-glutamate (L-Glu) by glutamate decarboxylase (GAD). Particularly, most bacterial GADs are robust only under acidic conditions and sharply lose their activity when shifted to near-neutral pH. In this work, to broaden the pH dependence of GadB from Levilactobacillus brevis, five C-termina ...
DOI:
10.1016/j.procbio.2022.04.036

9. Immobilization and characterization of latex cysteine peptidases on different supports and application for cow’s milk protein hydrolysis

Author:
João P.B. Oliveira; Luciana R.B. Gonçalves; Kímberle P.S. Amorim; Bruna B. Pinheiro; Márcio V. Ramos; Pedro F.N. Souza; Jefferson S. Oliveira; Deborah C. Freitas; Cleverson D.T. Freitas
Source:
Process biochemistry 2022 v.117 pp. 180-190
ISSN:
1359-5113
Subject:
Calotropis procera; allergenicity; biocatalysts; catalytic activity; cheesemaking; cows; cysteine; enzyme activity; enzyme kinetics; latex; milk; milk proteins; pH stability; peptidases; proteolysis; rennet; whey
Abstract:
... Calotropis procera cysteine peptidases (CpCPs) have been used for reducing cow’s milk allergenicity or as rennet in cheesemaking. Due to their residual presence in food products, the present study evaluated, for the first time, different protocols for their immobilization on different supports. Although the yield of immobilization on sulfopropyl-agarose (99%, at pH 7.0) was better than when using ...
DOI:
10.1016/j.procbio.2022.04.005

10. Immobilization of xylanase on differently functionalized silica gel supports for orange juice clarification

Author:
Dilek Alagöz; Nazli Ece Varan; Ali Toprak; Deniz Yildirim; S. Seyhan Tukel; Roberto Fernandez-Lafuente
Source:
Process biochemistry 2022 v.113 pp. 270-280
ISSN:
1359-5113
Subject:
Trichoderma longibrachiatum; catalytic activity; chemical bonding; clarification (processing); glutaraldehyde; ion exchange; orange juice; pH; silica gel; thermal stability; thermogravimetry; xylanases
Abstract:
... The immobilization of xylanase from Trichoderma longibrachiatum via ion exchange on 3-propylsulfonic acid (Xyl/Si-SO₃) and 3-aminopropyl (Xyl/Si-NH₃) functionalized silica gel and covalently on 3-carboxypropyl (Xyl/Si−COOH) and silica gel functionalized with 3-aminopropyl and modified with glutaraldehyde (Xyl/Si-Glu) were performed and the prepared biocatalysts were used for clarification of orang ...
DOI:
10.1016/j.procbio.2021.12.027

11. Immobilized glucosyltransferase and sucrose synthase on Fe3O4@Uio-66 in cascade catalysis for the one-pot conversion of rebaudioside D from rebaudioside A

Author:
Xiaojie Liu; Yifan Hu; Bin Wei; Fang Liu; Haichang Xu; Changxia Liu; Ye Li; Hao Liang
Source:
Process biochemistry 2022 v.118 pp. 323-334
ISSN:
1359-5113
Subject:
Arabidopsis thaliana; Escherichia coli; Fourier transform infrared spectroscopy; Oryza sativa; catalytic activity; electron microscopy; industrial applications; magnetic susceptibility; magnetism; nanocomposites; pH; sucrose synthase; sugars; temperature; thermogravimetry
Abstract:
... Rebaudioside D (RD) is a natural low-calorie, high-sweet sweetener and an excellent sugar substitute. In this study, RD was produced by coupling glucosyltransferase (EUGT11) and sucrose synthase (SUS) with a novel strategy of immobilization to enhance the recyclability of enzymes and decrease the production costs of RD. Recombinant EUGT11 from Oryza sativa and SUS from Arabidopsis thaliana were ex ...
DOI:
10.1016/j.procbio.2022.05.004

12. Introducing a covalent thiol-based protected immobilized acetylcholinesterase with enhanced enzymatic performances for biosynthesis of esters

Author:
Saeed Reza Hormozi Jangi; Morteza Akhond
Source:
Process biochemistry 2022 v.120 pp. 138-155
ISSN:
1359-5113
Subject:
acetylcholinesterase; biocatalysts; biosynthesis; catalytic activity; esterification; immobilized enzymes; magnetism; pH; temperature
Abstract:
... Acetylcholinesterase covalent immobilization was performed on mercaptoacetic acid-functionalized magnetic nanoparticles. Upon the thiol-based protection of the enzyme active center, its kinetics, stability, and activity in organic solvents were enhanced and its initial activity was fully recovered. The optimal results for the immobilization (i.e., yield=98.3 ± 5.2 %; enzyme loading=1.20 ± 0.06 mg  ...
DOI:
10.1016/j.procbio.2022.06.004

13. N-truncation in lipase Lip11 from Yarrowia lipolytica alleviates substrate inhibition with improved stability and efficiency ensuing distinct structural modifications

Author:
Amuliya Kashyap; Rani Gupta
Source:
Process biochemistry 2022 v.116 pp. 185-196
ISSN:
1359-5113
Subject:
Yarrowia lipolytica; acid tolerance; carboxylic ester hydrolases; castor oil; catalytic activity; half life; hydrophobicity; pH; polyacrylamide gel electrophoresis; substrate specificity; temperature; thermal stability; ultraviolet radiation
Abstract:
... Four sequential N-truncations in the 70-residue extended terminus of lipase Lip11 yielded truncated variants T1 (Δ18), T2 (Δ35), T3 (Δ58), and T4 (Δ70). T1 and T2 instigated 35% reduction in activity, 20 U/mg to 12.5 U/mg; while N-truncating 58 residues generated 3.3-fold catalytically efficient variant T3 (Vₘₐₓ = 43 U/mg), and further truncation (T4) emerged detrimental. Additionally, N-truncatio ...
DOI:
10.1016/j.procbio.2022.03.013

14. Purification and characterization of isocitrate dehydrogenase from Mortierella alpina

Author:
Xuxu Wang; Xin Tang; Haiqin Chen; Hao Zhang; Yong Q. Chen; Jianxin Zhao; Wei Chen
Source:
Process biochemistry 2022 pp. -
ISSN:
1359-5113
Subject:
Mortierella alpina; catalytic activity; fatty acids; isocitrate dehydrogenase; isozymes; oleaginous fungi; pH
Abstract:
... Isocitrate dehydrogenase (IDH) in oleaginous microorganisms plays an essential role in fatty acid synthesis. We expressed and purified an IDH isozyme from the oleaginous fungus Mortierella alpina (MaIDH4) and identified its enzymatic properties. MaIDH4 is NADP⁺-dependent and has no detectable activity with NAD⁺. It exerts its maximum activity when Mn²⁺ is used as a cofactor, followed by Mg²⁺. The ...
DOI:
10.1016/j.procbio.2022.07.031

15. Transglycosylation by β-mannanase TrMan5A variants and enzyme synergy for synthesis of allyl glycosides from galactomannan

Author:
Samuel J. Butler; Simon Birgersson; Mathias Wiemann; Monica Arcos-Hernandez; Henrik Stålbrand
Source:
Process biochemistry 2022 v.112 pp. 154-166
ISSN:
1359-5113
Subject:
Komagataella pastoris; Trichoderma reesei; active sites; allyl alcohol; biocompatible materials; catalytic activity; galactomannans; glycosides; glycosylation; hydrolysis; locust beans; mass spectrometry; mutants; pH; polymers
Abstract:
... Retaining β-mannanases are glycoside hydrolases (GHs) that can potentially be applied for synthesis of glycosides by catalysis of transglycosylation reactions. A novel active-site double mutant (R171K/E205D) of the catalytic module (CM) of the family GH5 Trichoderma reesei β-mannanase (TrMan5A) was expressed in Pichia pastoris and purified. TrMan5A, CM and CM-variants R171K and R171K/E205D had pH ...
DOI:
10.1016/j.procbio.2021.11.028

16. Z. Officinale-Doped Silver/Calcium Oxide Nanocomposites: Catalytic Activity and Antimicrobial Potential with Molecular Docking Analysis

Author:
Zakariya Mehmood; Muhammad Ikram; Muhammad Imran; Anum Shahzadi; Ali Haider; Anwar Ul-Hamid; Walid Nabgan; Junaid Haider; Shaukat Hayat
Source:
Process biochemistry 2022 pp. -
ISSN:
1359-5113
Subject:
DNA topoisomerase (ATP-hydrolysing); Escherichia coli; Staphylococcus aureus; Zingiber officinale; antimicrobial properties; calcium oxide; catalytic activity; ciprofloxacin; dairy industry; dihydrofolate reductase; methylene blue; nanocomposites; plant extracts; rhizomes; toxicity; Pakistan
Abstract:
... In this study, green synthesized Ag/CaO combined with a reducing agent based on a plant extract was synthesized. Then, the catalytic and antimicrobial potential of extract doped-Ag/CaO was investigated. Bio-reducing synthesis protocol was followed to fabricate Ag/CaO nanocomposites (NCs) by reducing and capping with Zingiber officinale. A series of characterization techniques was employed to exami ...
DOI:
10.1016/j.procbio.2022.07.035

17. A novel concept of Pyridoxal 5'-phosphate permeability in E.coli for modulating the heterologous expression of PLP dependent proteins

Author:
Vijay Kumar Saxena; G.V. Vedamurthy; Raghvendar Singh
Source:
Process biochemistry 2022 v.113 pp. 37-46
ISSN:
1359-5113
Subject:
catalytic activity; dephosphorylation; drugs; fluorescence; heterologous gene expression; permeability; phosphates; pyridoxal; pyridoxal phosphate; recombinant proteins; tryptophan
Abstract:
... PLP (Pyridoxal 5′-phosphate) dependent proteins are important drug targets and effector molecules, and thus, their heterologous overexpression is of industrial importance and has commercial value. We have predicted the docking site of PLP on O-acetylserine sulphdrylase (OASS) of H.contortus and determined that the lysine-47 is very important for the binding of PLP. We have used this protein as a m ...
DOI:
10.1016/j.procbio.2021.12.016

18. CityApps: A bioinformatics tool for predicting the key residues of enzymes weakly interacting with monovalent metal ions

Author:
Cheng Shi; Song Liu; Guocheng Du
Source:
Process biochemistry 2021 v.104 pp. 76-82
ISSN:
1359-5113
Subject:
Pseudomonas; bioinformatics; catalytic activity; hydrolases; mutants; mutation; parathion-methyl; solvents
Abstract:
... Monovalent metal ions play an essential role in some enzyme reactions. Due to the loose interactions, identifying the critical residues interacting with monovalent metal ions based on a molecular dynamic (MD) analysis is still a challenge. In this study, we first defined the enrichment factor (Eₐₐ) and the dwell time (td) to describe the interactions with metal ions in each enzyme residue during M ...
DOI:
10.1016/j.procbio.2021.03.005

19. Cloning and heterologous expression of subtilisin SAPN, a serine alkaline protease from Melghiribacillus thermohalophilus Nari2AT in Escherichia coli and Pichia pastoris

Author:
Sondes Mechri; Nadia Zaraî Jaouadi; Khelifa Bouacem; Fawzi Allala; Aicha Bouraoui; Céline Ferard; Hatem Rekik; Alexandre Noiriel; Abdelkarim Abousalham; Amel Bouanane-Darenfed; Hocine Hacène; Florence Lederer; Laura Baciou; Bassem Jaouadi
Source:
Process biochemistry 2021 v.105 pp. 27-41
ISSN:
1359-5113
Subject:
Bacillus licheniformis; Escherichia coli; Komagataella pastoris; catalytic activity; crabs; genes; heterologous gene expression; hydrolysis; pH; sequence analysis; serine; shrimp; subtilisin; temperature
Abstract:
... The sapN gene, encoding the extracellular subtilisin SAPN, a serine alkaline protease from Melghiribacillus thermohalophilus Nari2Aᵀ, was isolated, sequenced, and heterologously expressed in Escherichia coli BL21(DE3)pLysS using pUT57 and pTrc99A vectors and in E. coli BL21-AI™ using the Gateway™ pDEST™ 17 vector. Conversely, three cassettes encoding pre-pro-subtilisin (rSAPN/SP-Pro-M), pro-subtil ...
DOI:
10.1016/j.procbio.2021.03.020

20. Cobalt-doped MoS2 nanocomposite with NADH oxidase mimetic activity and its application in colorimetric biosensing of NADH

Author:
Yujiao Zhang; Yuanyuan Cai; Jin Wang; Lingxi Niu; Shuqing Yang; Xuan Liu; Zongmei Zheng; Lingxing Zeng; Aihua Liu
Source:
Process biochemistry 2021 v.111 pp. 178-185
ISSN:
1359-5113
Subject:
catalytic activity; colorimetry; detection limit; molybdenum; nanocomposites; oxidation; oxygen; pH; peroxidase; temperature
Abstract:
... NADH oxidase (NOX) is an oxidoreductase that catalyzes the oxidation of NADH to NAD⁺ with the participation of oxygen. NOX can artificially regulate metabolic processes as an important regulatory enzyme for NAD⁺ regeneration. In this study, cobalt-doped molybdenum sulfide (Co-MoS₂) nanocomposite was prepared by hydrothermal synthesis, which exhibited NOX mimics. The catalytic mechanism of NOX mimi ...
DOI:
10.1016/j.procbio.2021.09.009

21. Functional characterization of the extra sequence in the large subunit of γ-glutamyl transpeptidase from Bacillus atrophaeus: Role in autoprocessing and activity

Author:
Meenu Saini; Shalini Kalra; Jai K. Kaushik; Rani Gupta
Source:
Process biochemistry 2021 v.106 pp. 199-212
ISSN:
1359-5113
Subject:
Bacillus atrophaeus; catalytic activity; computer simulation; consensus sequence; sequence alignment; thermal stability
Abstract:
... Bacterial γ-glutamyl transpeptidases (GGTs) exhibit differences in their catalytic properties due to distinct structural variability. Comparative sequence alignment of some well-characterized bacterial GGTs indicated marked differences at C-terminus in the large subunit of enzyme. An extra sequence of 14 amino acids was identified from genus Bacillus that was distinctly absent in other GGTs. Detai ...
DOI:
10.1016/j.procbio.2021.05.004

22. High performance nanozymatic assay-based CuO nanocluster supported by reduced graphene oxide for determination of hydrogen peroxide and ascorbic acid

Author:
Fatemeh Honarasa; Rooza Mokhtare; Azadeh Mokhtare; Saeed Yousefinejad
Source:
Process biochemistry 2021 v.111 pp. 256-261
ISSN:
1359-5113
Subject:
absorbance; ascorbic acid; bovine serum albumin; catalytic activity; color; copper sulfate; detection limit; enzymes; graphene oxide; hydrogen peroxide; nanocomposites; oxidation; stabilizers; synergism; ultraviolet-visible spectroscopy
Abstract:
... Several efforts have been made to increase the nanozymatic activity of reduced Graphene Oxide (rGO). Also, the enzyme mimetic research with Nanoclusters (NCs) is in the embryo stage. For the first time, a one pot synthesis method was used successfully for preparation of CuONCs loaded r-GO (CuONCs/rGO). GO and copper sulfate were used as precursors and Bovine Serum Albumin (BSA) as the reducer and ...
DOI:
10.1016/j.procbio.2021.10.025

23. Immobilization of phytase on zeolite modified with iron(II) for use in the animal feed and food industry sectors

Author:
Marina Momesso Lopes; Thamara Carvalho Coutinho; João Otávio Donizette Malafatti; Elaine Cristina Paris; Cristina Paiva de Sousa; Cristiane Sanchez Farinas
Source:
Process biochemistry 2021 v.100 pp. 260-271
ISSN:
1359-5113
Subject:
animal nutrition; catalytic activity; feeds; food industry; hydrolysis; ion exchange capacity; ionic strength; iron; pH; phytases; phytic acid; soybean meal; soymilk; wheat bran; zeolites
Abstract:
... Here, we investigated the feasibility of immobilizing phytase onto zeolite modified with iron(II) ions, for application in the feed and food sectors. Zeolites have excellent physical-chemical characteristics, ion exchange capacity, and can act as dietary supplements. The phytase immobilization yield was up to 73 % at pH 7 and ionic strength of 100 mM, with recovered activity higher than 100 %, rep ...
DOI:
10.1016/j.procbio.2020.10.017

24. Multi-efficient thermostable endoxylanase from Bacillus velezensis AG20 and its production of xylooligosaccharides as efficient prebiotics with anticancer activity

Author:
Arabinda Ghosh; Anshuman Chandra; Avinash Dhar; Pratyoosh Shukla; Debabrat Baishya
Source:
Process biochemistry 2021 v.109 pp. 59-71
ISSN:
1359-5113
Subject:
Bacillus velezensis; Bifidobacterium; alpha-amylase; antineoplastic activity; calcium; catalytic activity; cell proliferation; density functional theory; endo-1,4-beta-xylanase; enzymatic hydrolysis; enzyme activity; gastric juice; human cell lines; intestinal secretions; molecular dynamics; pH; prebiotics; probiotics; sugarcane bagasse; temperature; thermal stability; xylooligosaccharides
Abstract:
... In this study, we have unveiled the novel potential of xylanase production by Bacillus velezensis, previously known only for its PGPR traits. According to our investigations, Bacillus velezensis AG20 produced extracellular xylanase with a fold purification of 5.3 and a yield of 21 %. The purified xylanase (45 kDa) aptly cleaved linear β-(1→4)-xylan with a maximum velocity of 21.0 ± 3.0 U/mL, a tur ...
DOI:
10.1016/j.procbio.2021.06.011

25. Overexpression and mutation of a novel lipase from Serratia marcescens L1 in Escherichia coli

Author:
Haiqing Chen; Fan Yu; Nan Shi; Pingping Du; Shuang Liu; Xianzhou Zhang; Jianxin Tan
Source:
Process biochemistry 2021 v.111 pp. 233-240
ISSN:
1359-5113
Subject:
Escherichia coli; Serratia marcescens; amino acids; carboxylic ester hydrolases; catalytic activity; genes; mutation; pH; thermal stability
Abstract:
... A novel lipase gene (lipA) from Serratia marcescens L1 was cloned and expressed in Escherichia coli. LipA was shown to consist of 614 amino acid residues with seven residues that are different from the other nine reported lipases from S. marcescens. The activity of recombinant lipA based on a 50-mL culture scale approached 28 U/mL, which was more than that of the endogenous extracellular lipase fr ...
DOI:
10.1016/j.procbio.2021.11.001

26. Protein engineering of a cold-adapted rhamnogalacturonan acetylesterase: In vivo functional expression and cinnamyl acetate synthesis

Author:
Xu-dong Tang; Feng-ying Dong; Qi-hao Zhang; Lin Lin; Ping Wang; Xiang-yang Xu; Wei Wei; Dong-zhi Wei
Source:
Process biochemistry 2021 v.107 pp. 129-137
ISSN:
1359-5113
Subject:
Escherichia coli; Paenibacillus polymyxa; acetates; alcohols; catalytic activity; enzymes; genes; mutants; pH; phosphates; site-directed mutagenesis; temperature; thermal stability
Abstract:
... The rhamnogalacturonan acetylesterase gene (named pp1113), encoding a novel cold-adapted rhamnogalacturonan acetylesterase, was cloned from Paenibacillus polymyxa. pp1113 was functionally expressed in Escherichia coli by inducing expression at 20 °C for 16 h. The pH and temperature optimums for the recombinant enzyme were 8.5 and 30 °C, respectively. As an SGNH-type acetylesterase, pp1113 demonstr ...
DOI:
10.1016/j.procbio.2021.05.011

27. Purification and biochemical characterization of two novel extracellular keratinases with feather-degradation and hide-dehairing potential

Author:
Bilal Kerouaz; Bassem Jaouadi; Alain Brans; Boudjema Saoudi; Amina Habbeche; Soumeya Haberra; Hafedh Belghith; Ali Gargroui; Ali Ladjama
Source:
Process biochemistry 2021 v.106 pp. 137-148
ISSN:
1359-5113
Subject:
Actinomadura; ammonium sulfate; anion exchange chromatography; catalytic activity; fluorides; fractionation; heat treatment; high performance liquid chromatography; hydrolysis; leather industry; pH; polyacrylamide gel electrophoresis; serine proteinases; sodium dodecyl sulfate; substrate specificity; thermal stability
Abstract:
... Two novel extracellular keratinases were produced by Actinomadura keratinilytica strain Cpt20. Both enzymes were purified to homogeneity using heat-treatment (60 °C for 30 min) and ammonium sulfate salt fractionation (40 %–70 %), followed by anion-exchange chromatography with fast protein liquid chromatography (FPLC) system. The purified keratinases, designated as KERA-71 and KERB-19, are monomeri ...
DOI:
10.1016/j.procbio.2021.04.009

28. Rational protein engineering of α-L-arabinofuranosidase from Aspergillus niger for improved catalytic hydrolysis efficiency on kenaf hemicellulose

Author:
Nardiah Rizwana Jaafar; Siti Norbaidurah Ayob; Noor Hidayah Abd Rahman; Farah Diba Abu Bakar; Abdul Munir Abdul Murad; Rosli Md Illias
Source:
Process biochemistry 2021 v.102 pp. 349-359
ISSN:
1359-5113
Subject:
Aspergillus niger; Hibiscus cannabinus; active sites; biomass; catalytic activity; enzymatic hydrolysis; fossils; hemicellulose; hydrolysis; hydrophobicity; kenaf; lignocellulose
Abstract:
... Lignocellulosic biomass utilisation as an alternative to fossil resources was hampered due to its recalcitrant to enzymatic hydrolysis. Herein, rational protein engineering strategy has been carried out on Aspergillus niger α-L-arabinofuranosidase (AnabfA) substrate binding pathway to improve its catalytic efficiency towards pre-treated kenaf (Hibiscus cannabinus) hemicellulose hydrolysis. A total ...
DOI:
10.1016/j.procbio.2020.12.012

29. Rice bran oil deacidification by immobilized Aspergillus Niger lipase catalyzed esterification with D-isoascorbic acid

Author:
Fan Yang; Mengyang Wang; Xinyu Chao; Xiang Yan; Wencheng Zhang; Chuanxun Yuan; Qingmei Zeng
Source:
Process biochemistry 2021 v.111 pp. 219-226
ISSN:
1359-5113
Subject:
Aspergillus niger; acid value; carboxylic ester hydrolases; catalytic activity; deacidification; esterification; phytochemicals; rice bran oil; shelf life
Abstract:
... The main challenge associated with the upgrading of crude rice bran oil (RBO) rests with their high content of free fatty acids (FFA). In this study, we describe a green and sustainable process for the deacidification of high-acid RBO using d-isoascorbic acid as a novel acyl acceptor in combination with a newly prepared immobilized Aspergillus Niger lipase (ANL). The process contributed a high dea ...
DOI:
10.1016/j.procbio.2021.10.013

30. Structure analysis and inhibition mechanism of peroxidase in 'Zhongshu 1' sweet potato

Author:
Fengmao Li; Yufan Fu; Jiaxin Li; Yunming Tang
Source:
Process biochemistry 2021 v.100 pp. 161-170
ISSN:
1359-5113
Subject:
active sites; ascorbic acid; catalytic activity; chromatography; fluorescence; glutathione; guaiacol; industrialization; mass spectrometry; peroxidase; sodium metabisulfite; sweet potatoes
Abstract:
... Browning is an obstacle in industrial development of sweet potato. Peroxidase from sweet potato cv. ‘Zhongshu 1′ was purified and analyzed by chromatography and mass spectrometry. Peroxidase from ‘Zhongshu 1′ sweet potato was a monomeric protein with 37.2 kDa. A binding site of peroxidase to guaiacol was discovered using molecular docking, with Arg61 and Ala191 being near the active region importa ...
DOI:
10.1016/j.procbio.2020.10.004

31. Synergistic action of cyclodextrin glucanotransferase and maltogenic amylase improves the bioconversion of starch to malto-oligosaccharides

Author:
Nardiah Rizwana Jaafar; Rabi’atul Adawiyah Ahmad; Noor Namirah Nawawi; Noor Hidayah Abd Rahman; Nur Arbainah Shamsul Annuar; Roshanida A. Rahman; Rosli Md Illias
Source:
Process biochemistry 2021 v.103 pp. 9-17
ISSN:
1359-5113
Subject:
amylases; biotransformation; catalytic activity; consciousness; cyclodextrins; cyclomaltodextrin glucanotransferase; oligosaccharides; pH; starch; supply balance; synergism; temperature
Abstract:
... Overwhelming consumer consciousness for healthier food has led to high market demand for functional oligosaccharides. These functional sugars can be extracted from various sources, especially starch, through enzymatic approaches. Maltogenic amylases are able to synthesise malto-oligosaccharides (MOS) from starch. However, the use of maltogenic amylase alone presents several limitations such as low ...
DOI:
10.1016/j.procbio.2021.02.002

32. -Identification of a novel intestinal phospholipase A2 from annular seabream: Insights into its catalytic mechanism and its role in biological processes

Author:
Nabil Smichi; Houcemeddine Othman; Zied Zarai; Ahmed Fendri; Abdelkarim Abousalham
Source:
Process biochemistry 2020 v.91 pp. 197-207
ISSN:
1359-5113
Subject:
Diplodus annularis; amino acids; catalytic activity; cytotoxicity; digestion; fish; hydrolysis; intestines; macrophages; mammals; matrix-assisted laser desorption-ionization mass spectrometry; molecular weight; phosphatidylcholines; phosphatidylethanolamines; phospholipase A2; phospholipases; polyacrylamide gel electrophoresis; thermal stability
Abstract:
... Phospholipase A₂ (PLA₂) is responsible for the lipid hydrolysis process. Fish PLA₂ have warranted renewed interest due to their excellent properties in phospholipid digestion. We report for the first time the catalytic properties of a PLA₂ secreted from the intestine of the annular seabream Diplodus annularis (IDaPLA₂). The refolded IDaPLA₂ was purified to homogeneity and showed a molecular mass o ...
DOI:
10.1016/j.procbio.2019.12.012

33. Autocatalytic hydrothermal pretreatment by recycling byproduct organic acids to directionally depolymerize cassava straw

Author:
Huisheng Lyu; Ziwei Zhai; Jinyi Zhou; Jia Zhang; Chunliu Lyu; Zhi Wang; Zhongfeng Geng
Source:
Process biochemistry 2020 v.88 pp. 97-105
ISSN:
1359-5113
Subject:
acetic acid; byproducts; cassava; catalytic activity; enzymatic hydrolysis; formic acid; lactic acid; liquids; recycling; straw; sugars; temperature
Abstract:
... A two-stage autocatalytic hydrothermal pretreatment was proposed to improve the cassava straw utilization. The two-stage hydrothermal pretreatment was a process of which the first stage adopted lower-severity conditions (temperature and time) to improve the C-5 sugar yields and the second stage employed more severities to enhance C-6 sugar yield during enzyme hydrolysis. After employing this proce ...
DOI:
10.1016/j.procbio.2019.10.013

34. Biochemical characterization of a novel hyperthermophilic α-l-rhamnosidase from Thermotoga petrophila and its application in production of icaritin from epimedin C with a thermostable β-glucosidase

Author:
Jingcong Xie; Shanshan Zhang; Xinyi Tong; Tao Wu; Jianjun Pei; Linguo Zhao
Source:
Process biochemistry 2020 v.93 pp. 115-124
ISSN:
1359-5113
Subject:
Thermotoga; alpha-L-rhamnosidase; antineoplastic activity; beta-glucosidase; biotransformation; carbon; catalytic activity; drugs; flavonoids; moieties; molecular cloning; pH; temperature; thermal stability; thermophilic microorganisms; thermostable enzymes
Abstract:
... Epimedin C, a major flavonoid extracted from Herba Epimedii, is a precursor of minor flavonoid icaritin that is a desired drug candidate with remarkable anti-cancer activities. For enhancing the biotransformation efficiency of icaritin, a novel α-l-rhamnosidase gene was cloned from hyperthermophiles Thermotoga petrophila DSM 13995. TpeRha displayed optimal activity at a pH of 4.5 and a temperature ...
DOI:
10.1016/j.procbio.2020.03.019

35. Biodegradation of organophosphorus insecticides by two organophosphorus hydrolase genes (opdA and opdE) from isolated Leuconostoc mesenteroides WCP307 of kimchi origin

Author:
Azizul Md. Haque; Chung Eun Hwang; Su Cheol Kim; Du Yong Cho; Hee Yul Lee; Kye Man Cho; Jin Hwan Lee
Source:
Process biochemistry 2020 v.94 pp. 340-348
ISSN:
1359-5113
Subject:
Escherichia coli; Leuconostoc mesenteroides; amino acids; aryldialkylphosphatase; biodegradation; butyrates; cabbage; cadusafos; catalytic activity; chlorpyrifos; diazinon; ethoprophos; fenamiphos; fermentation; genes; kimchi; molecular weight; pH; parathion
Abstract:
... This study is aimed to reveal the molecular incidence of organophosphorus insecticides degradation during the fermentation of Korean food yeulmu-mulkimchi. To this end, two opdA and opdE which consist of 930 and 894 bp that encode 309 and 297 amino acids, respectively, were cloned from the Leuconostoc mesenteroides WCP307 strain that was isolated from chlorpyrifos (CP) impregnated kimchi. The Esch ...
DOI:
10.1016/j.procbio.2020.04.026

36. Characterization of a robust glucose 1-dehydrogenase, SyGDH, and its application in NADPH regeneration for the asymmetric reduction of haloketone by a carbonyl reductase in organic solvent/buffer system

Author:
Die Hu; Zheng Wen; Chuang Li; Bochun Hu; Ting Zhang; Jianfang Li; Minchen Wu
Source:
Process biochemistry 2020 v.89 pp. 55-62
ISSN:
1359-5113
Subject:
DNA; Escherichia coli; NADP (coenzyme); affinity chromatography; biocatalysts; catalytic activity; dimethyl sulfoxide; enzymes; genes; glucose; hexane; nucleotide sequences; organochlorine compounds; pH; phosphates; solvents; temperature
Abstract:
... To realize coenzyme regeneration in the reduction of haloketones, a codon-optimized gene Sygdh encoding glucose 1-dehydrogenase (SyGDH) was synthesized based on the putative GDH gene sequence (Ta0897) in Thermoplasma acidophilum genomic DNA, and expressed in E. coli BL21(DE3). Recombinant SyGDH was purified to homogeneity by affinity chromatography with the specific activity of 86.3 U/mg protein t ...
DOI:
10.1016/j.procbio.2019.09.037

37. Characterization of chitinase from Shewanella inventionis HE3 with bio-insecticidal effect against granary weevil, Sitophilus granarius Linnaeus (Coleoptera: Curculionidae)

Author:
Hassiba Laribi-Habchi; Khelifa Bouacem; Fawzi Allala; Fadoua Jabeur; Okba Selama; Sondes Mechri; Merzouk Yahiaoui; Amel Bouanane-Darenfed; Bassem Jaouadi
Source:
Process biochemistry 2020 v.97 pp. 222-233
ISSN:
1359-5113
Subject:
Shewanella; Sitophilus granarius; biological control; catalytic activity; chitin; chitinase; enzyme activity; epithelial cells; fermenters; freeze drying; glycosides; histology; larvae; molecular weight; mortality; pH; peritrophic membrane; storage quality; temperature; Algeria
Abstract:
... A 40 kDa chitinase from Shewanella inventionis HE3 was purified (ChiA-Si40) and characterized. Using fermentor with an optimized medium for 48 h at 37 °C, enzyme activity was enhanced by 10-times compared to those using shaking-flask-culture. Purified chitinase is a homogenous monomer with molecular mass of 40 kDa. Its N-terminal residues revealed significant identity with glycoside hydrolase fami ...
DOI:
10.1016/j.procbio.2020.06.023

38. Degradative GH5 β-1,3-1,4-glucanase PpBglu5A for glucan in Paenibacillus polymyxa KF-1

Author:
Ye Yuan; Xinyu Zhang; Han Zhang; Weiyang Wang; Xuesong Zhao; Juan Gao; Yifa Zhou
Source:
Process biochemistry 2020 v.98 pp. 183-192
ISSN:
1359-5113
Subject:
Paenibacillus polymyxa; antifungal properties; barley; carboxymethylcellulose; catalytic activity; curdlan; endo-1,4-beta-glucanase; glucooligosaccharides; glycosides; glycosylation; licheninase; oat bran; polymerization; substrate specificity
Abstract:
... A novel β-1,3-1,4-glucanase in the glycoside hydrolase family 5 (GH5) has been identified in the secretome of Paenibacillus polymyxa KF-1. The recombinant GH5 enzyme PpBglu5A shows broad substrate specificity, with strong lichenase activity, medium β-1,3-glucanase activity, and minimal cellulase activity. Barley β-glucan, lichenan, curdlan, and carboxymethyl cellulose are hydrolyzed to varying deg ...
DOI:
10.1016/j.procbio.2020.08.008

39. Development, validation and enzyme kinetic evaluation of multi walled carbon nano tubes mediated tyrosinase based electrochemical biosensing platform for the voltammetric monitoring of epinephrine

Author:
P. Gopal; G. Narasimha; T. Madhusudana Reddy
Source:
Process biochemistry 2020 v.92 pp. 476-485
ISSN:
1359-5113
Subject:
biosensors; blood serum; carbon nanotubes; catalytic activity; central nervous system; detection limit; dielectric spectroscopy; diffusivity; electrochemistry; enzyme kinetics; enzymes; epinephrine; glassy carbon electrode; humans; monitoring; neurotransmitters; pH; phosphates; voltammetry
Abstract:
... Epinephrine (EP) is one of the key neurotransmitter, which plays a vital role in the central nervous system. Current research report designates the development of biosensor based on the modification of glassy carbon electrode (GCE) with multi walled carbon nano tubes (MWCNTs) followed by drop casting of Tyrosinase (Ty) enzyme (Ty/MWCNTs/GCE) towards the sensitive monitoring of EP. The electrochemi ...
DOI:
10.1016/j.procbio.2020.02.006

40. Directing cell catalysis of glucose to 2-keto-d-gluconic acid using Gluconobacter oxydans NL71

Author:
Xuelian Zhou; Yi Shen; Yong Xu; Venkatesh Balan
Source:
Process biochemistry 2020 v.94 pp. 365-369
ISSN:
1359-5113
Subject:
Gluconobacter oxydans; biorefining; catalytic activity; corn stover; enzymes; gene expression regulation; genes; gluconates; glucose; hydrogen peroxide; hydrolysates; lignocellulose; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction
Abstract:
... 2-Keto-d-gluconic acid (2-KGA) is a high-value-added product from lignocellulosic straw-based biorefining process obtained via cell catalysis using Gluconobacter oxydans (G. oxydans); nonetheless, the economical production has some limitations because of the formation of undesirable mixture products of keto-gluconic acid from glucose. In this study, it was demonstrated that pulse addition of hydro ...
DOI:
10.1016/j.procbio.2020.04.038

41. Effect of protein adsorption on bioelectrochemistry of electrospun core-shell MWCNTs/gelatin-Hb nanobelts on electrode surface

Author:
Z.X. Deng; J.W. Tao; L.J. Zhao; W. Zhang; Y.B. Wang; H.J. Mu; H.J. Wu; X.X. Xu; W. Zheng
Source:
Process biochemistry 2020 v.96 pp. 73-79
ISSN:
1359-5113
Subject:
adsorption; biochemistry; bioelectrochemistry; biosensors; carbon; catalytic activity; contact angle; electron transfer; glassy carbon electrode; hydrogen peroxide; hydrophilicity
Abstract:
... Implantable electrochemical biosensor is one powerful tool for the accurate and reliable measurements of small molecules in vivo. However, the electrode is inevitably subjected to the protein adsorption when implanted into the living animals, affecting the sensitivity and stability of biosensor. Herein, we designed the multi-walled carbon nanotubes/gelatin-hemoglobin (MWCNTs/gelatin-Hb) core-shell ...
DOI:
10.1016/j.procbio.2020.05.031

42. Efficient synthesis of (3R,5S)-6-chloro-2,4,6-trideoxyhexapyranose by using new 2-deoxy-d-ribose-5-phosphate aldolase from Streptococcus suis with moderate activity and aldehyde tolerance

Author:
Kaiang Xuan; Guangyi Yang; Zhimeng Wu; Yan Xu; Rongzhen Zhang
Source:
Process biochemistry 2020 v.92 pp. 113-119
ISSN:
1359-5113
Subject:
Escherichia coli; Streptococcus suis; acetaldehyde; catalytic activity; fructose-bisphosphate aldolase; pH; sequence alignment
Abstract:
... The enzyme 2-deoxy-d-ribose-5-phosphate aldolase (DERA) is a useful tool for synthesizing statin side-chain intermediates. In this work, we identified the DERA from Streptococcus suis (SsDERA) by structural and sequence alignment and highly expressed it in Escherichia coli BL21. The recombinant SsDERA had a specific activity of 18.2 U mg⁻¹, KM of 0.8 mM, and Vₘₐₓ of 32.9 μmol min⁻¹ mg⁻¹ toward 2-d ...
DOI:
10.1016/j.procbio.2020.03.006

43. Enantioselective chemoenzymatic synthesis of (R)-γ-valerolactone from levulinic acid

Author:
Dohoon Lee; Young Joo Yeon
Source:
Process biochemistry 2020 v.90 pp. 113-117
ISSN:
1359-5113
Subject:
3-hydroxybutyrate dehydrogenase; bioactive compounds; biofuels; biomass; catalytic activity; enantioselectivity; lactones; levulinic acid; sulfuric acid; value-added products
Abstract:
... A number of chemicals with high industrial value can be synthesized from levulinic acid, a feasible building block readily available from cellulosic biomass. Among them, γ-valerolactone is a versatile chemical precursor for the synthesis of value-added products including bio-active molecules, bio-fuels, and carbon-based chemicals. In this study, a novel two-step chemoenzymatic conversion of levuli ...
DOI:
10.1016/j.procbio.2019.11.019

44. Engineering a colanic acid biosynthesis pathway in E. coli for manufacturing 2’-fucosyllactose

Author:
Qiao Chen; Haiying Wu; Minghua Ji; Yukang Xie; Sijie Li; Yiqing Li; Jiping Shi; Junsong Sun
Source:
Process biochemistry 2020 v.94 pp. 79-85
ISSN:
1359-5113
Subject:
Escherichia coli; biosynthesis; biotransformation; breast milk; catalytic activity; engineering; fucose; glycerol; heterologous gene expression; infant formulas; infants; lactose; manufacturing; pH; polyhydroxybutyrate; transferases
Abstract:
... Human milk oligosaccharides (HMOs) are beneficial for infants’ health and growth. As one of the most abundant oligosaccharides in human milk, 2′-fucosyllactose (2′-FL) has been approved to supplement in infant formula. Microbial synthesis of 2′-FL achieved in E. coli tends to use a T7-expression system for the heterologous expression of the fucosyltransferase and/or enzymes involved in fucose meta ...
DOI:
10.1016/j.procbio.2020.04.017

45. Enzymatic Bioconversion of Cycloastragenol-6-O-β-D-glucoside into Cycloastragenol by a Novel Recombinant β-Glucosidase from Phycicoccus sp. Soil748

Author:
Leiyu Cheng; Han Zhang; Hao Liang; Xinxiao Sun; Xiaolin Shen; Jia Wang; Wenya Wang; Qipeng Yuan; Hyon-Il Ri; Tae-Mun Kim; Myong-Su Kang; Robert J. Linhardt
Source:
Process biochemistry 2020 v.90 pp. 81-88
ISSN:
1359-5113
Subject:
Astragalus membranaceus; astragalosides; beta-glucosidase; biotransformation; catalytic activity; kinetics; markets; pH; temperature
Abstract:
... Cycloastragenol (CA), the genuine sapogenin of astragaloside from Astragalus membranaceus, exhibits diverse pharmaceutical activities. Recently, the efficient production of CA has received considerable attention due to rapidly increasing market demands. In this study, enzyme mining was conducted, based on skeleton and glycosyl similarity, to explore an efficient β-glucosidase for CA preparation. A ...
DOI:
10.1016/j.procbio.2019.11.006

46. Enzymatic production of 4-hydroxyphenylacetaldehyde by oxidation of the amino group of tyramine with a recombinant primary amine oxidase

Author:
Tianqi Yu; Yifan Yin; Yihe Ge; Shiwei Cheng; Xingxiao Zhang; Zhibin Feng; Juan Zhang
Source:
Process biochemistry 2020 v.92 pp. 105-112
ISSN:
1359-5113
Subject:
Bacillus pumilus; Escherichia coli; biocatalysts; biotransformation; catalase; catalytic activity; ethyl acetate; oxidation; pH; solvents; temperature; tyramine
Abstract:
... In this study, an efficient enzymatic process for the synthesis of 4-hydroxyphenylacetaldehyde (4-HPAA) from tyramine was developed using whole cells of recombinant Escherichia coli co-expressing primary amine oxidase (PrAO) from E. coli and catalase (CAT) from Bacillus pumilus. The reaction conditions for the synthesis of 4-HPAA were systematically optimized starting from a monophasic aqueous buf ...
DOI:
10.1016/j.procbio.2020.03.007

47. Enzymatic synthesis of an orlistat intermediate using a mutant short-chain dehydrogenase from Novosphingobium aromaticivorans

Author:
Yunping Tang; Yafeng Zhou; Qiaojun Zhao; Qiaoling Zhao; Zhao Wang
Source:
Process biochemistry 2020 v.95 pp. 235-241
ISSN:
1359-5113
Subject:
Sphingomonas; anti-obesity agents; catalytic activity; computer simulation; enzymes; gas chromatography; mutants
Abstract:
... Methyl (R)-3-hydroxytetradeconoate ((R)-MHOT) is a crucial chiral intermediate for the chemical synthesis of the anti-obesity drug, orlistat. Here, (R)-MHOT was prepared from methyl 3-oxotetradecanoate (MOT) using a mutant of the short-chain dehydrogenase/reductase (SDR) from Novosphingobium aromaticivorans (NaSDR). Mutant NaSDR-G145A/I199L had a 3.23 times greater kcₐₜ value than that of wild typ ...
DOI:
10.1016/j.procbio.2020.02.015

48. Gene cloning, expression and biochemical characterization of a new multi-domain, halotolerant and SDS-resistant alkaline pullulanase from Alkalibacterium sp. SL3

Author:
Han Huang; Yun Lin; Guozeng Wang; Juan Lin
Source:
Process biochemistry 2020 v.96 pp. 1-10
ISSN:
1359-5113
Subject:
Alkalibacterium; Escherichia coli; alpha-amylase; amino acids; carbohydrate binding; catalytic activity; detergents; genes; pH; pullulanase; remediation; salt tolerance; signal peptide; thermal stability
Abstract:
... A new pullulanase-encoding gene, pulSL3, was cloned from Alkalibacterium sp. SL3 and expressed in Escherichia coli. Deduced PulSL3 contains 2026 amino acid residues and consists of a putative signal peptide, four carbohydrate-binding modules, an E_set pullulanase domain, an α-amylase domain, and a pullulanase domain. The purified rPulSL3 exhibited pullulanase activity only. To identify the functio ...
DOI:
10.1016/j.procbio.2020.05.019

49. Green biogenic approach to optimized biosynthesis of noble metal nanoparticles with potential catalytic, antioxidant and antihaemolytic activities

Author:
Megha P. Desai; Reshma V. Patil; Kiran D. Pawar
Source:
Process biochemistry 2020 v.98 pp. 172-182
ISSN:
1359-5113
Subject:
Pseudomonas stutzeri; aminophenols; antioxidant activity; antioxidants; bacteria; biosynthesis; catalysts; catalytic activity; p-nitrophenol; pH; palladium; platinum; spectroscopy; temperature
Abstract:
... Owing to a wide range of potential applications of noble metal nanoparticles such as palladium nanoparticles (PdNPs) and platinum nanoparticles (PtNPs), their simpler and eco-friendly synthesis has attracted the scientific community worldwide. This study demonstrates bacterium Pseudomonas stutzeri mediated simple, eco-friendly and optimized green method for the biogenic synthesis of PdNPs and PtNP ...
DOI:
10.1016/j.procbio.2020.08.005

50. Haloacid dehalogenases of Rhizobium sp. and related enzymes: Catalytic properties and mechanistic analysis

Author:
Aliyu Adamu; Roswanira Abdul Wahab; Firdausi Aliyu; Aminu Hamza Aminu; Mariya Musa Hamza; Fahrul Huyop
Source:
Process biochemistry 2020 v.92 pp. 437-446
ISSN:
1359-5113
Subject:
Rhizobium; amino acids; bioremediation; carbon; catalytic activity; dehalogenation; enzymes; halogens; organic acids and salts
Abstract:
... Haloacid dehalogenases catalyse the cleavage of carbon − halogen bonds in halogenated organic acids. These enzymes are of high interest due to their potential applications in bioremediation and in synthesis of various industrial products. The efficiency of dehalogenases in various applications can be enhanced, provided that their molecular catalytic mechanisms are fully understood. Herein, we revi ...
DOI:
10.1016/j.procbio.2020.02.002