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You searched for: Journal Reproduction, fertility, and development Remove constraint Journal: Reproduction, fertility, and development Publication Year 2021 Remove constraint Publication Year: 2021 Source 2021 v.33 no.2 Remove constraint Source: 2021 v.33 no.2
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1. 118 A comparison of intravaginal progesterone devices for fixed-time artificial insemination in beef cattle

Author:
Zwiefelhofer, E. M.; Yang, S. X.; Asai-Coakwell, M.; Colazo, M. G.; Hellquist, J.; Zwiefelhofer, M. L.; Anzar, M.; Adams, G. P.
Source:
Reproduction, fertility, and development 2021 v.33 no.2 pp. 167
ISSN:
1031-3613
Subject:
animal health; artificial insemination; beef; beef cattle; cloprostenol; color; drugs; estrus; gonadotropin-releasing hormone; ovulation; pregnancy; progesterone; ultrasonography; Saskatchewan
Abstract:
... Intravaginal progesterone (P4) devices used for ovarian synchronization before fixed-time AI (FTAI) differ in drug release, which may influence fertility outcome. A 2×2 study was designed to determine the effects of different intravaginal devices (PRID Delta, 1.55g of P4 vs. CIDR, 1.38g of P4) and parity (heifers vs. cows) on follicular dynamics, expression of oestrus, and pregnancy per AI (P/AI). ...
DOI:
10.1071/RDv33n2Ab118

2. 107 Administration of antioxidants improves serum total antioxidant capacity, testosterone, and seminal quality of Magra rams

Author:
Kumar, A.; Mehta, J. S.; Kumar, A.; Purohit, G. N.
Source:
Reproduction, fertility, and development 2021 v.33 no.2 pp. 160-161
ISSN:
1031-3613
Subject:
antioxidant activity; ascorbic acid; blood serum; computer software; glutathione peroxidase; hydrogen peroxide; male sterility; meat; oxidative stress; pH; selenium; semen quality; seminal plasma; sodium selenite; sperm concentration; sperm motility; spermatozoa; superoxide dismutase; testosterone; vitamin E; wool; India
Abstract:
... Oxidative stress is one of the most important factors responsible for poor quality of semen, and it is defined as an imbalance between the formation of reactive oxygen species (ROS, including hydroxyl radicals, superoxide anions, and hydrogen peroxide) and antioxidant capacity of semen. Antioxidants act as a powerful defence system against ROS attack in sperm cells by decreasing oxidative stress a ...
DOI:
10.1071/RDv33n2Ab107

3. 40 Administration of 0.1μM melatonin during invitro maturation of bovine oocytes regulates autophagy levels in produced embryos

Author:
El-Sheikh, M.; Mesalam, A. A.; Lee, K.-L.; Kong, I.-K.
Source:
Reproduction, fertility, and development 2021 v.33 no.2 pp. 127
ISSN:
1031-3613
Subject:
apoptosis; autophagy; blastocyst; caspase-3; cattle; fluorescent antibody technique; melatonin; metalloproteinases; oocytes; phosphatidylinositol 3-kinase; protective effect; reactive oxygen species; reproduction; t-test; toxicity
Abstract:
... Melatonin, the antioxidant pineal hormone, is a strong regulator for various cellular processes essential for reproduction. Although the protective role of 0.1µM melatonin against the toxicity of different anti-developmental compounds has been elucidated in numerous studies, its effect on the autophagy level in invitro-produced blastocysts has not been entirely clarified. In this study, oocytes we ...
DOI:
10.1071/RDv33n2Ab40

4. 102 Age-related differences in seminal parameters and expression of fertility marker genes in Marwari stallion

Author:
Chaudhary, A. K.; Purohit, G. N.; Mehta, J. S.; Ravi, S. K.; Talluri, T. R.
Source:
Reproduction, fertility, and development 2021 v.33 no.2 pp. 158
ISSN:
1031-3613
Subject:
DNA; analysis of variance; artificial vagina; color; computer software; gels; gene expression regulation; mares; pH; plasma membrane; quantitative polymerase chain reaction; sperm concentration; sperm motility; spermatozoa; stallions; viability
Abstract:
... The objective of the present study was to observe age-related differences in seminal parameters and expression of fertility marker genes in Marwari stallions. Semen was collected from 9 Marwari stallions (6 ejaculates per stallion) of 3 different age groups (2 to 4 years, GI; 4 to 6 years, GII; and >6 years, GIII) twice a week using an artificial vagina by allowing the stallions to mount a mare. T ...
DOI:
10.1071/RDv33n2Ab102

5. 36 Analysis of miRNA content of oviduct and uterine extracellular vesicles across the bovine estrous cycle

Author:
Hamdi, M.; Mazzarella, R.; Cañon-Beltrán, K.; Cajas, Y. N.; Leal, C. L. V.; Gutiérrez-Adán, A.; González, M. E.; da Silveira, J. C.; Rizos, D.
Source:
Reproduction, fertility, and development 2021 v.33 no.2 pp. 125
ISSN:
1031-3613
Subject:
bioinformatics; conceptus; corpus luteum; databases; estrous cycle; gel chromatography; ice; microRNA; oviducts; quantitative polymerase chain reaction; uterus
Abstract:
... With the aim of investigating the possible hormonal regulatory effect of the oestrous cycle on miRNA content in the extracellular vesicles (EVs) of bovine oviducal and uterine fluids (OF, UF), we performed a bioinformatic analysis of these miRNAs, their target genes, and their biological pathways. Reproductive tracts were collected from slaughtered heifers and selected according to their corpus lu ...
DOI:
10.1071/RDv33n2Ab36

6. 32 An approach to invitro oocyte maturation and fertilization in threatened Saharan Dorcas gazelle (Gazella dorcas osiris) using frozen-thawed epididymal sperm cells

Author:
Ruiz-Conca, M.; Gardela, J.; Álvarez-Rodríguez, M.; Fernández-Bellon, H.; López-Béjar, M.
Source:
Reproduction, fertility, and development 2021 v.33 no.2 pp. 123
ISSN:
1031-3613
Subject:
Gazella dorcas; Sahel; adults; air; cattle; centrifugation; cryoprotectants; embryo culture; endangered species; eosin; epididymis; extrusion; females; freeze-thaw cycles; liquid nitrogen; males; necropsy; oocytes; semen; semen extenders; spermatozoa; viability; zygote
Abstract:
... The Dorcas gazelle (Gazella dorcas), in the family Bovidae, is one of the smallest species of the genus Gazella. Its population is decreasing and it is classified as vulnerable by the International Union of Conservation of Nature Red List of Threatened Species. The subspecies Saharan Dorcas gazelle (G. dorcas osiris), originating in the central-western Saharan and Sahel regions, is almost extinct ...
DOI:
10.1071/RDv33n2Ab32

7. 119 Anti-Müllerian hormone as an endocrine marker of fertility in Bos taurus heifers

Author:
Torres-Quijada, I. F.; Romo-Garcia, S.; Zamorano-Algandar, R.; Torres-Simental, J. F.
Source:
Reproduction, fertility, and development 2021 v.33 no.2 pp. 167-168
ISSN:
1031-3613
Subject:
Simmental; anti-Mullerian hormone; blood sampling; blood serum; body condition; conception; estrus; linear models; prostaglandins; ultrasonography
Abstract:
... The objective of the study was to determine the relationship between the serum concentration of anti-Mullerian hormone (AMH) and fertility indicators in Bos taurus heifers subjected to breeding. From a group of 40 Simmental heifers (15–18 months of age, body condition score of 5 to 7; weight 350–400kg) that received a single dose of 25mg of dinoprost, 23 females entered oestrus and were artificial ...
DOI:
10.1071/RDv33n2Ab119

8. 121 Anti-Müllerian hormone cutoff values for the selection of oocyte donors in the Gir (Bos indicus) breed

Author:
Feres, L. F.; Siqueira, L. G. B.; Pfeifer, L F. M.; Santos, L. L.; Viana, J. H. M.
Source:
Reproduction, fertility, and development 2021 v.33 no.2 pp. 168-169
ISSN:
1031-3613
Subject:
anti-Mullerian hormone; blastocyst; blood sampling; estrous cycle; oocytes; ovarian follicles; zebu; Brazil
Abstract:
... Anti-Müllerian hormone (AMH) has a well-known association with antral follicle count (AFC) and the number of oocytes recovered by OPU in cattle. However, to be used as a criterion to select candidate donors for ART, AMH reference values must be established for each breed. The aim of this study was to estimate AMH cutoff values for selecting oocyte donors in Gir (Bos indicus) cattle. A blood sample ...
DOI:
10.1071/RDv33n2Ab121

9. 7 A record of 485 viable cumulus–oocyte complexes recovered and 165 viable embryos produced in a single ovum pickup session from a Senepol breed donor

Author:
Resende, A. O.; Bohrer, R. C.; Viana, J. H. M.
Source:
Reproduction, fertility, and development 2021 v.33 no.2 pp. 111
ISSN:
1031-3613
Subject:
Senepol; beef; blastocyst; catheters; estrous cycle; farms; heifers; oocytes; ovarian follicles; progeny; rural areas; semen; sires; ultrasonics; Brazil
Abstract:
... The recovery of an exceptionally high number of cumulus–oocyte complexes (COC) in a single ovum pickup (OPU) session is rare, and thus seldom reported in the literature. In the present report, we describe one of these cases. The present data refers to an OPU session performed by author A. O. Resende in a commercial beef farm, located in the rural area of the city of Barretos, Brazil, on October 15 ...
DOI:
10.1071/RDv33n2Ab7

10. 12 Artificial insemination and embryo transfer results in ewes during a long daylength period

Author:
Mittleider, T.; Collins, S.; Gibbons, P.; Gibbons, J.
Source:
Reproduction, fertility, and development 2021 v.33 no.2 pp. 113-114
ISSN:
1031-3613
Subject:
artificial insemination; embryo transfer; estrus; field experimentation; genetic improvement; gonadotropin-releasing hormone; photoperiod; progeny; progesterone; prostaglandins; semen; summer; ultrasonography; Virginia
Abstract:
... Sheep are polyestrous, short-day breeders (∼11h of daylength), and exhibit oestrus approximately every 16–17 days during the breeding season, usually in late September to late December in the Northern Hemisphere. Progressive sheep producers often use assisted reproductive techniques such as laparoscopic AI and ovarian hyper-stimulation, embryo collection, and embryo transfer (ET) to increase genet ...
DOI:
10.1071/RDv33n2Ab12

11. Artificially produced gametes in mice, humans and other species

Author:
Hayashi, Katsuhiko; Galli, Cesare; Diecke, Sebastian; Hildebrandt, Thomas B.
Source:
Reproduction, fertility, and development 2021 v.33 no.2 pp. 91-101
ISSN:
1031-3613
Subject:
gametogenesis; germ cells; medicine; mice
Abstract:
... The production of gametes from pluripotent stem cells in culture, also known as invitro gametogenesis, will make an important contribution to reproductive biology and regenerative medicine, both as a unique tool for understanding germ cell development and as an alternative source of gametes for reproduction. Invitro gametogenesis was developed using mouse pluripotent stem cells but is increasingly ...
DOI:
10.1071/RD20265

12. 4 Aryl hydrocarbon receptor targets are upregulated in porcine blastocyst-stage embryos that were cultured invitro: A transcriptional analysis

Author:
Chen, P. R.; Spate, L. D.; Spollen, W. G.; Cecil, R. F.; Samuel, M. S.; Prather, R. S.
Source:
Reproduction, fertility, and development 2021 v.33 no.2 pp. 109
ISSN:
1031-3613
Subject:
; Sus scrofa; amino acids; aryl hydrocarbon receptors; cell cycle; cytochrome P-450; gene expression regulation; metabolism; oviducts; reproduction; transcription (genetics); uterus; xenobiotics
Abstract:
... Invitro-produced (IVP) porcine embryos are developmentally delayed compared with those derived invivo. Thus, efforts have been made to modify the current medium for IVP of porcine embryos in an attempt to shift the abundance of target transcripts towards the invivo level. The objective of the current study was to identify differences in mRNA abundance that may account for reduced developmental com ...
DOI:
10.1071/RDv33n2Ab4

13. 93 A sheep model of sickle cell disease using CRISPR/Cas9 and somatic cell nuclear transfer

Author:
Perisse, I. V.; Almeida-Porada, G.; Porada, C. D.; White, K. L.; Polejaeva, I. A.
Source:
Reproduction, fertility, and development 2021 v.33 no.2 pp. 154
ISSN:
1031-3613
Subject:
CRISPR-Cas systems; DNA; RNA; animal models; computer software; exons; fetal bovine serum; fibroblasts; gene editing; glutamic acid; hemoglobin; homozygosity; humans; immunity; oligodeoxyribonucleotides; oxygen; penicillins; people; phenotype; reproduction; restriction endonucleases; ribonucleoproteins; sheep; sickle cell anemia; somatic cells; valine
Abstract:
... Sickle cell disease (SCD) is the most common inherited hemoglobinopathy, with more than 2 million people in the United States alone carrying the sickle gene. Approximately 100 000 of these people are homozygous and suffer from SCD. Worldwide, there are ∼4.4 million people with SCD. SCD is caused by a single A to T nucleotide replacement at the sixth codon of the β-globin gene, which results in the ...
DOI:
10.1071/RDv33n2Ab93

14. 23 Biopsied invitro-produced bovine blastocysts survive vitrification better than slow freezing

Author:
Najafzadeh, V.; Secher, J.; Andersen, A.; Jørgensen, N.; Strøbech, L.; Hyttel, P.
Source:
Reproduction, fertility, and development 2021 v.33 no.2 pp. 119
ISSN:
1031-3613
Subject:
DNA; biopsy; blastocyst; cattle; computer software; cryopreservation; ethylene glycol; oocytes; slaughterhouses; sucrose; t-test; vitrification
Abstract:
... Trophectoderm (TE) biopsying for single nucleotide polymorphisms (SNPs) analysis is being implemented as a tool for the selection of elite bovine embryos. This biopsy method renders ample cells for analysis without compromising the inner cell mass (ICM), and the blastocyst recovers quickly after biopsy. To use the SNP data for embryo selection before the transfer, the blastocysts need to be cryopr ...
DOI:
10.1071/RDv33n2Ab23

15. 15 Blastocysts altered CDX2 and SOX2 gene expression and pregnancy failure after embryo transfer in yak heterospecific somatic cell nuclear transfer

Author:
Felipe, M. Y.; Rodríguez, M. D.; Ratner, L. D.; De Stéfano, A.; Valdez, A. M.; Salamone, D. F.
Source:
Reproduction, fertility, and development 2021 v.33 no.2 pp. 115
ISSN:
1031-3613
Subject:
actin; blastocyst; electrofusion; embryo transfer; embryonic mortality; genetic rescue; oocytes; parthenogenesis; phytohemagglutinin; pregnancy; proteinases; quantitative polymerase chain reaction; somatic cells; ultrasonics; yaks; zona pellucida
Abstract:
... Heterospecific cloning is a tool for the genetic rescue of endangered animals. Our objective was to evaluate the effects of heterospecific yak (Bos grunniens) cloned embryo aggregation on the expression levels of NANOG, OCT4, CDX2, and SOX2 genes, and to compare with IVF, parthenogenetic zona-free (P-ZF), and homospecific bovine cloned embryos (BB1x). Oocytes were recovered from the ovaries of sla ...
DOI:
10.1071/RDv33n2Ab15

16. 74 Body condition of donor animal and recipients affects the outcome of embryo transfer program in dromedary camel (Camelus dromedarius)

Author:
Vettical, B. S.; Wani, N. A.
Source:
Reproduction, fertility, and development 2021 v.33 no.2 pp. 144-145
ISSN:
1031-3613
Subject:
Camelus dromedarius; body condition; conception; embryo transfer; energy balance; equine chorionic gonadotropin; gonadotropin-releasing hormone; intramuscular injection; males; ovulation; pregnancy; ultrasonics; uterus
Abstract:
... The present study was conducted in dromedary camels to evaluate the effect of the body condition of donor animals on the outcome of superstimulation and embryo collection in experiment 1 and the establishment of pregnancies in the recipients in experiment 2. The animals were physically evaluated for their body condition and scored on a scale of 0 to 5 (0 being very thin and weak, while 5 being ver ...
DOI:
10.1071/RDv33n2Ab74

17. 34 Cellular effects of antifreeze proteins type I and III in extender for sheep semen cryopreservation

Author:
Correia, L. F. L.; Espírito-Santo, C. G.; Braga, R. F.; Brair, V. L.; de Paula, C. J. C.; da Silva, A. A.; Brandão, F. Z.; Freitas, V. J. F.; Ungerfeld, R.; Souza-Fabjan, J. M. G.
Source:
Reproduction, fertility, and development 2021 v.33 no.2 pp. 124
ISSN:
1031-3613
Subject:
acrosome; antifreeze proteins; chromatin; cryopreservation; cryoprotectants; freeze-thaw cycles; lipid peroxidation; liquid nitrogen; mitochondria; plasma membrane; rams; reactive oxygen species; refrigeration; semen extenders; semen quality; sperm capacitation; sperm quality
Abstract:
... Antifreeze proteins (AFP) have been included in extenders for sperm cryopreservation to prevent ice crystal formation. Thus, this study assessed the effects of supplementing semen extender with two concentrations of AFP types I and III on the quality of frozen–thawed ram sperm. The hypothesis is that various types and concentrations of AFP enhance cryopreservation of ram sperm. Semen was collected ...
DOI:
10.1071/RDv33n2Ab34

18. 150 Changes in pyruvate metabolism alters the epigenetic and molecular maturation of bovine oocytes

Author:
Silva, J. V. A.; Ispada, J.; Junior, A. M. Fonseca; dos Santos, E. C.; de Lima, C. B.; da Rocha, H. C.; Milazzotto, M. P.
Source:
Reproduction, fertility, and development 2021 v.33 no.2 pp. 183-184
ISSN:
1031-3613
Subject:
acetyl coenzyme A; acetylation; cattle; computer software; epigenetics; estradiol; fetal bovine serum; fluorescence microscopes; glycolysis; histones; human chorionic gonadotropin; oocytes; pyruvic acid; reproduction; sodium; t-test; transcription (genetics)
Abstract:
... During invitro maturation (IVM), bovine oocytes undergo important metabolic, epigenetic, and transcriptional changes for the acquisition of developmental competence. Particularly, metabolic changes that alter the availability of cytoplasmic acetyl-CoA, the main substrate for histones acetylation, may alter the epigenetic profile of the oocyte, with consequences for correct molecular maturation. To ...
DOI:
10.1071/RDv33n2Ab150

19. 84 Characterization of agouti-signalling protein expression within the bovine ovary and early embryo

Author:
Baldwin, H.; Zhang, M.; Current, J.; Yao, J.
Source:
Reproduction, fertility, and development 2021 v.33 no.2 pp. 150
ISSN:
1031-3613
Subject:
adipokines; adults; blastocyst; cattle; cerebral cortex; embryogenesis; gene expression; genes; germinal vesicle; heart; insulin resistance; intestines; kidneys; liver; lungs; melanocytes; muscles; obesity; pigmentation; pregnancy; protein secretion; protein synthesis; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; ribosomal proteins; stomach; testes
Abstract:
... Factors present in the oocyte and surrounding follicular cells aid in the attainment of oocyte competence. Agouti-signalling protein (ASIP) is a known regulator of melanocyte function through binding to melanocortin receptors including MC1R and MC4R. Additionally, ASIP has been classified as an adipokine due to a link with insulin resistance and obesity in humans. In mice, expression is limited to ...
DOI:
10.1071/RDv33n2Ab84

20. 35 Characterization of the promoter region of ZNFO, an oocyte-specific gene in cattle

Author:
Zhang, M.; Baldwin, H.; Current, J.; Yao, J.
Source:
Reproduction, fertility, and development 2021 v.33 no.2 pp. 125
ISSN:
1031-3613
Subject:
RNA; bioinformatics; cattle; embryogenesis; germinal vesicle; leucine zipper; luciferase; plasmids; promoter regions; reproduction; transcription factors; transcription initiation site; zinc finger motif
Abstract:
... Maternal factors are essential aspects of oocyte competence, which orchestrate early embryonic development. ZNFO is a Krüppel-associated box domain (KRAB) containing zinc finger transcription factor, which is exclusively expressed in bovine oocyte. Previous studies have demonstrated that ZNFO is essential for early embryonic development. However, the mechanisms regulating ZNFO transcription remain ...
DOI:
10.1071/RDv33n2Ab35