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You searched for: Journal Reproduction, fertility, and development Remove constraint Journal: Reproduction, fertility, and development Publication Year 2020 Remove constraint Publication Year: 2020 Source 2020 v.32 no.2 Remove constraint Source: 2020 v.32 no.2
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1. 110 Absence of transmission of Mycoplasma bovis via naturally contaminated semen during invitro fertilization

Author:
Peippo, J.; Vähänikkilä, N.; Mutikainen, M.; Lindeberg, H.; Pohjanvirta, T.; Simonen, H.; Pelkonen, S.; Autio, T.
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 182
ISSN:
1031-3613
Subject:
Mycoplasma bovis; air; antibiotic resistance; blastocyst; bovine serum albumin; bulls; bursitis; carbon dioxide; culture media; embryogenesis; fatty acids; fetal bovine serum; follicle-stimulating hormone; heat inactivation; heparin; humidity; insemination; oocytes; oxygen; penicillins; quantitative polymerase chain reaction; salpingitis; semen; sodium pyruvate; spermatozoa; streptomycin; tissue culture; trypsin; uterus; vaccines; zygote
Abstract:
... Mycoplasma bovis (Mbo) has been isolated from genital tracts of bulls, and it can survive in processed semen. Experimental studies have shown that Mbo inoculation into the uterus or insemination with Mbo-infected semen can cause bursitis, salpingitis, abortion, and infertility. The control of Mbo is very difficult because of latent carrier animals, increasing resistance to antibiotics, and unavail ...
DOI:
10.1071/RDv32n2Ab110

2. 43 Actions of colony-stimulating factor 3 on the maturing oocyte and developing embryo in cattle

Author:
Jannaman, E.; Xiao, Y.; Hansen, P.
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 147
ISSN:
1031-3613
Subject:
blastocyst; cattle; embryogenesis; embryonic germ layers; gene expression; genes; granulocyte colony-stimulating factor; in vitro fertilization; messenger RNA; oocytes; patients; pregnancy outcome; reverse transcriptase polymerase chain reaction; semen; swine; zygote
Abstract:
... Colony-stimulating factor 3 (CSF3), also known as granulocyte colony-stimulating factor, has been reported to improve blastocyst yield when administered during oocyte maturation in the pig and to increase pregnancy outcomes in specific IVF patient populations when infused transvaginally. Here, we tested whether recombinant bovine CSF3 can act on the bovine oocyte during maturation or on the develo ...
DOI:
10.1071/RDv32n2Ab43

3. 102 A delay in maternal zygotic transition may lead to early embryonic loss in poor-quality bovine blastocysts

Author:
Georges, H.; Bishop, J.; Van Campen, H.; Barfield, J.; Hansen, T.
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 177
ISSN:
1031-3613
Subject:
bioinformatics; blastocyst; blastomeres; dairy cows; death; embryo implantation; embryo transfer; embryonic mortality; endometrium; enzymatic hydrolysis; enzymes; estradiol; gene expression; gene expression regulation; genes; genetic traits; in vitro fertilization; industry; messenger RNA; mice; mitosis; oocytes; permeability; pregnancy; pregnenolone; progesterone; proteins; risk; sequence analysis; transcription (genetics); translation (genetics); zona pellucida
Abstract:
... Increased genetic potential and performance of dairy cows has coincided with a decline in fertility. Early embryonic mortality accounts for 75-80% of this decline in fertility, costing the industry over $1.28 trillion worldwide. Despite advancements in assisted reproductive technologies and embryo transfer, many transferred embryos do not survive past Day 24 of gestation, suggesting flaws in embry ...
DOI:
10.1071/RDv32n2Ab102

4. 20 Aggregation of yak heterospecific somatic cell nuclear transfer embryos improves cloning efficiency

Author:
Yauri Felipe, M.; Duque Rodríguez, M.; De Stéfano, A.; Salamone, D.
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 135-136
ISSN:
1031-3613
Subject:
Bos; air; animal ovaries; blastocyst; bulls; carbon dioxide; cows; culture media; cysteamine; electrofusion; embryogenesis; endangered species; fetal bovine serum; follicle-stimulating hormone; in vitro fertilization; oocytes; parthenogenesis; phytohemagglutinin; proteinases; slaughterhouses; sodium pyruvate; somatic cells; staining; tissue culture; wells; yaks; zona pellucida
Abstract:
... Cloning endangered species has the limitation that generally the number of available oocytes is limited. Reprogramming the nuclei heterospecifically using an enucleated oocyte from a different species is an alternative. Aggregation of SCNT (somatic cell nuclear transfer) embryos from the same specie results in improved embryo development. However, after aggregation of heterospecific SCNT embryos f ...
DOI:
10.1071/RDv32n2Ab20

5. 154 A method of oviductal semen deposition for use in the goat

Author:
Buzzell, N.; Blash, S.; Miner, K.; Schofield, M.; Pollock, J.; Hawkins, N.; Hevy, M.; Gavin, W.
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 203-204
ISSN:
1031-3613
Subject:
Saanen; Toggenburg; cattle; cryopreservation; dairy goats; fetus; follicle-stimulating hormone; genetically modified organisms; gonadotropin-releasing hormone; in vitro fertilization; isoflurane; laparotomy; oviducts; pellets; progeny; progesterone; prostaglandins; semen; sperm concentration; sperm quality; straw; superovulation; syringes; tuberculin; uterus; Massachusetts
Abstract:
... The objective of this study was to investigate a method of oviducal semen deposition as a strategy for producing offspring from poor-quality cryopreserved goat sperm. Invitro fertilisation (IVF) and AI are common assisted reproductive technologies used in small ruminants, but they have varied results in the goat. The use of poor-quality cryopreserved-thawed sperm (<50% live/dead ratio at post-thaw ...
DOI:
10.1071/RDv32n2Ab154

6. 44 Analysis of abnormal chromatin configuration induced by inhibiting MEK at the 1-cell stage

Author:
Magara, K.; Naruto, S.; Watanabe, R.; Wakayama, T.; Kishigami, S.
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 147-148
ISSN:
1031-3613
Subject:
blastocyst; cell nucleolus; epigenetics; females; heterochromatin; histone code; in vitro fertilization; insemination; males; meiosis; mice; mitogen-activated protein kinase; oocytes; superovulation; transcription (genetics)
Abstract:
... After fertilisation, mammalian oocytes resume meiosis, leading to pronuclear (PN) formation. It has been known that the mitogen-activated protein kinase pathway negatively regulates PN formation. However, it remains largely unknown how the mitogen-activated protein kinase pathway is involved in the dynamic organisation of chromatin structure in PNs. Here, to address how MEK1/2 activity is involved ...
DOI:
10.1071/RDv32n2Ab44

7. 74 Analysis of chromosomal abnormality of bovine IVF embryos based on next-generation sequencing

Author:
Okada, M.; Nagai, Y.; Matoba, S.; Sakuraba, Y.; Sugimura, S.
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 163
ISSN:
1031-3613
Subject:
DNA; DNA libraries; Wagyu; X chromosome; aneuploidy; bioinformatics; blastocyst; blood serum; calves; carbon dioxide; chromosome duplication; copy number variation; cows; culture dishes; genome; high-throughput nucleotide sequencing; in vitro fertilization; insemination; loci; males; nucleotide sequences; oxygen; pregnancy
Abstract:
... It has been suggested that bovine IVF embryos have a higher frequency of occurrence of chromosomal abnormalities than invivo-fertilised embryos, which may explain low pregnancy success, but the details have not been clarified (Yao et al. 2018 Sci. Rep. 8, 7460). In this study, chromosomal aneuploidy in blastocysts of bovine IVF and invivo-fertilised was analysed by copy number variations (CNVs) ba ...
DOI:
10.1071/RDv32n2Ab74

8. 87 Analyzing metabolomic profile of bovine IVF and somatic cell nuclear transfer embryos through Raman spectroscopy

Author:
Keim, J.; Zhang, W.; Liu, Y.; Rutigliano, H.; Zhou, A.; Polejaeva, I.
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 169-170
ISSN:
1031-3613
Subject:
Raman spectroscopy; animal ovaries; blastocyst; bovine serum albumin; carbon dioxide; cattle; chemical bonding; culture media; discriminant analysis; droplets; embryogenesis; fetal bovine serum; genes; human resources; in vitro culture; in vitro fertilization; least squares; metabolism; metabolomics; near infrared radiation; pregnancy rate; principal component analysis; somatic cells; viability; vibration
Abstract:
... While knowledge of early embryo development and viability has continually increased, there is still a need for noninvasive methods to identify embryos with the highest chances of development to term when transferred. The most widely used technique, morphological assessment, is highly subjective and limited by personnel experience. Spent media from invitro culture could be used as a valuable noninv ...
DOI:
10.1071/RDv32n2Ab87

9. 152 An effective method of inducing oestrus in superovulation-treated Japanese Black donor cows after egg collection

Author:
Aoyagi, Y.; Takeuchi, M.; Oono, T.; Hayama, K.; Urakawa, M.; Oono, Y.; Koiwa, M.
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 202-203
ISSN:
1031-3613
Subject:
Wagyu; chi-square distribution; conception rate; controlled internal drug release devices; cows; drugs; eggs; estrus; gonadotropin-releasing hormone; ova; prostaglandins; semen; superovulation; t-test; vagina
Abstract:
... The objective of this study was to evaluate the effect of inducing oestrus in superovulation-treated Japanese Black donor cows after egg collection using a controlled internal drug release (CIDR) device and prostaglandin F2α treatments. The test animals were 50 biparous or triparous Japanese Black cows that had been treated for superovulation during the period of 75-110 days post-calving and from ...
DOI:
10.1071/RDv32n2Ab152

10. 68 Anti-Mullerian hormone levels in different bovine species and the relationship with invitro embryo production

Author:
Berdugo, J.; Tarazona-Morales, A.; Echeverry-Zuluaga, J.; Cardona Maya, W.; López-Herrera, A.; Crudeli, G.; Konrad, J.; Espasandin, G.
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 159-160
ISSN:
1031-3613
Subject:
Bubalus bubalis; analysis of variance; animal ovaries; anti-Mullerian hormone; blastocyst; breeding season; buffaloes; bulls; cows; enzyme-linked immunosorbent assay; herds; industry; latitude; longitude; oocytes; semen; zebu
Abstract:
... The development of markers for reproductive function has been a goal for the embryo production industry and reproductive biology as a method to improve success and increase the ability to explain reproductive phenomena. Cattle and buffalo are bovines that show different reproductive parameters raised in the same conditions. The aim of this work was to compare the results of the invitro production ...
DOI:
10.1071/RDv32n2Ab68

11. Application of genome-editing systems to enhance available pig resources for agriculture and biomedicine

Author:
Lee, Kiho; Farrell, Kayla; Uh, Kyungjun
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 40-49
ISSN:
1031-3613
Subject:
CRISPR-Cas systems; animal models; cost effectiveness; embryo (animal); gene editing; genome; medicine; nucleases; somatic cells; swine; technology; zinc finger motif
Abstract:
... Traditionally, genetic engineering in the pig was a challenging task. Genetic engineering of somatic cells followed by somatic cell nuclear transfer (SCNT) could produce genetically engineered (GE) pigs carrying site-specific modifications. However, due to difficulties in engineering the genome of somatic cells and developmental defects associated with SCNT, a limited number of GE pig models were ...
DOI:
10.1071/RD19273

12. 25 A preliminary study of the effects of breed and nanowater as extender diluent on ram semen characteristics post-thawing

Author:
Murawski, M.; Szymanowicz, J.; Schwarz, T.; Bartlewski, P.
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 138-139
ISSN:
1031-3613
Subject:
Merino; alanine transaminase; alkaline phosphatase; analysis of variance; apoptosis; aspartate transaminase; chickens; droplets; egg yolk; flow cytometry; freeze-thaw cycles; genotype; mixing; nanotechnology; necrosis; rams; semen extenders; semen quality; spermatozoa; synthetic populations
Abstract:
... This study examined the effect of genotype and extender diluent on the characteristics of frozen-thawed ram semen. Twenty ejaculates collected from 10 rams aged 4-12 years (2 Polish Lowland (PON), 4 synthetic line BCP (Berrichon du Cher×Charolais×PON/Polish Merino), and 4 SCP (Suffolk×Charolais×PON/Polish Merino) in mid-breeding season were divided into six equal portions each and frozen in semen ...
DOI:
10.1071/RDv32n2Ab25

13. 1 Assessing the energy status of porcine embryos by means of biodynamic imaging

Author:
Lorenzo, I.; Li, Z.; Torres, M.; Machaty, Z.; Nolte, D.
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 125
ISSN:
1031-3613
Subject:
adenosine triphosphate; assisted reproductive technologies; bioluminescence assay; biomarkers; embryo (animal); energy; image analysis; microscopy; oocytes; oxidative phosphorylation; sodium azide; spectroscopy; swine; t-test; viability
Abstract:
... Assisted reproductive technologies are powerful tools for enhancing production in livestock or treating infertility in humans. Unfortunately, the success rate of the technologies is rather low. A major reason for the poor efficiency is the lack of methods to reliably assess the developmental potential of the embryos before transfer into recipients. Therefore, a noninvasive method to ensure the sel ...
DOI:
10.1071/RDv32n2Ab1

14. 12 Assessment of oocyte and embryo production in senescent beef cows

Author:
Alvarez, R. H.; Bayeux, B. M.; Watanabe, Y. F.; Joaquim, D. A.; Duarte, K. M. R.; Pugliesi, G.
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 131
ISSN:
1031-3613
Subject:
Nellore; amino acids; beef cows; blastocyst; calves; culture media; estradiol; funding; intravaginal devices; oocytes; prostaglandins; tissue culture; zygote
Abstract:
... Fertility associated with age has been poorly documented in cattle. There is evidence that more than 50% of cows became infertile (failure to bear a calf for two successive years) at the age of 15. The causes of infertility in cows have been attributed to poor oocyte quality and/or follicle depletion. The present study aimed to compare the rates of oocyte and invitro embryo production of fertile c ...
DOI:
10.1071/RDv32n2Ab12

15. 33 Assessment of spindle morphology and reactive oxygen species production after vitrification of bovine oocytes following invitro maturation in the presence of glutathione ethyl ester

Author:
García-Martínez, T.; Vendrell-Flotats, M.; Martínez-Rodero, I.; Álvarez-Rodríguez, M.; López-Béjar, M.; Mogas, T.
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 142
ISSN:
1031-3613
Subject:
air; carbon dioxide; cattle; chromosomes; cold tolerance; computer software; epidermal growth factor; fetal bovine serum; fluorescence; fluorescent antibody technique; glutathione; meiosis; microtubules; oocyte vitrification; oocytes; oxidative stress; reactive oxygen species; tissue culture; tubulin; vitrification; Spain
Abstract:
... Oocyte cryopreservation by vitrification affects spindle integrity and causes oxidative stress induced by reactive oxygen species (ROS). Glutathione (GSH) ensures the correct assembly of microtubules, prevents ROS from attacking tubulin assembly, and ensures normal spindle function during meiosis. We hypothesised that addition of GSH ethyl ester (GSH-OEt), a cell-permeable GSH donor, to IVM medium ...
DOI:
10.1071/RDv32n2Ab33

16. 138 Associations of sperm head morphometrics with quality parameters of frozen-thawed ram semen

Author:
Navaranjan, J.; Szymanowicz, J.; Murawski, M.; Schwarz, T.; Bartlewski, P. M.
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 195
ISSN:
1031-3613
Subject:
DNA; Merino; acrosome; alkaline phosphatase; artificial vagina; aspartate transaminase; biomarkers; breeding season; chromatin; computer software; conception; droplets; eosin; ewes; freeze-thaw cycles; membrane permeability; mitochondria; morphometry; multivariate analysis; nonthermal processing; ova; pregnancy rate; rams; semen extenders; seminal plasma; sperm motility; sperm quality; staining; straw; viability
Abstract:
... Current methods of mammalian semen evaluation focus on determining spermatozoa motility, concentration, mitochondrial status, and nucleus or chromatin structure integrity, quantifying their ability to bind to ova or measuring seminal plasma content of various biochemical markers. However, there is a paucity of studies that address relationships between sperm head morphometry (the external shape an ...
DOI:
10.1071/RDv32n2Ab138

17. 24 Asynchronic tetraploid complementation and embryo quality in domestic cat and Leopardus geoffroyi hybrid embryos

Author:
Duque Rodriguez, M.; Gambini, A.; Gutnisky, C.; Ratner, L.; Rulli, S.; Sestelo, A.; Briski, O.; Fernandez Martin, R.; Cetica, P. D.; Salamone, D.
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 138
ISSN:
1031-3613
Subject:
DNA; DNA fragmentation; Felis; Leopardus geoffroyi; aneuploidy; blastocyst; blastomeres; cats; electric field; embryo transfer; endangered species; females; fluorescent antibody technique; gene expression; hybrids; in vitro fertilization; karyotyping; nucleotidyltransferases; oocytes; ovariectomy; phylogeny; pregnancy; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; spermatozoa; tetraploidy
Abstract:
... Fusion of 2-cell embryos generates tetraploid (4n) blastomeres with an increased commitment to trophectoderm. Complementation of embryos from endangered species with 4n blastomeres derived from a phylogenetically related domestic species could improve healthy pregnancy establishment after embryo transfer in domestic females. However, generation, development, and quality of tetraploid complemented ...
DOI:
10.1071/RDv32n2Ab24

18. 26 Baobab oil supplemented extender preserves post-thaw bull sperm quality parameters

Author:
Raphalalani, Z.; Ramukhithi, F.; Ndhlala, R.; Nephawe, K.; Nedambale, T.
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 139
ISSN:
1031-3613
Subject:
Adansonia digitata; DNA; DNA damage; analysis of variance; antioxidant activity; antioxidants; bulls; cryopreservation; freezers; frozen storage; liquid nitrogen; nucleotidyltransferases; oils; oxidative stress; plant extracts; semen extenders; seminal plasma; sperm motility; sperm quality; straw; thawing; viability
Abstract:
... The processes of semen cryopreservation and thawing affect sperm membrane integrity and motility and increases morphological defects as well as DNA damage. The most influential cause of this is oxidative stress. When endogenous antioxidant capacity of seminal plasma is reduced during the freeze-thawing process, plant extracts exhibiting strong antioxidant activity can be used as supplements for co ...
DOI:
10.1071/RDv32n2Ab26

19. 182 Bisphenol A, but not bisphenol S, affects key microRNAs during bovine oocyte maturation

Author:
Sabry, R.; Nguyen, M.; Stalker, L.; LaMarre, J.; Favetta, L.
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 219
ISSN:
1031-3613
Subject:
Western blotting; acrylamides; actin; adverse effects; analysis of variance; animal reproduction; bisphenol A; bisphenol S; cattle; complementary DNA; densitometry; endocrine-disrupting chemicals; epigenetics; essential genes; females; gels; gene expression; mechanism of action; messenger RNA; microRNA; oocytes; protein content; quantitative polymerase chain reaction; sonication; toxicity; transcription (genetics)
Abstract:
... Oocyte maturation involves crucial hormone-dependent events that are uniquely susceptible to toxic insults by endocrine disrupting chemicals (EDCs). Emerging evidence suggests that small RNAs, including microRNAs (miRNAs), may be key participants in the response to EDCs. Bisphenol A (BPA) and bisphenol S (BPS) are chemicals with detrimental health effects, with BPA negatively affecting oocyte qual ...
DOI:
10.1071/RDv32n2Ab182

20. 179 Bisphenol S affects invitro early developmental oocyte competence in ewes

Author:
Desmarchais, A.; Téteau, O.; Papillier, P.; Elis, S.
Source:
Reproduction, fertility, and development 2020 v.32 no.2 pp. 217-218
ISSN:
1031-3613
Subject:
animal ovaries; bisphenol S; blastocyst; cell viability; chromatin; endocrine-disrupting chemicals; enzyme-linked immunosorbent assay; estrogen receptors; estrogens; ewes; fish; gene expression; humans; in vitro culture; in vitro fertilization; industry; linear models; meiosis; messenger RNA; mitogen-activated protein kinase; oocytes; phenotype; plastics; progesterone; quantitative polymerase chain reaction; regression analysis; rodents; secretion; signal transduction; staining; urine
Abstract:
... In the plastics industry, bisphenol S (BPS) replaces sisphenol A reported to be an oestrogen mimetic endocrine disruptor damaging oocyte meiosis and maturation (Machtinger 2014 Reprod. Biomed. Online 29, 404). Studies on fish and rodents reported that BPS affects reproduction similarly to BPA (Uzumcu 2007 Reprod. Toxicol. 23, 337; Giulivo 2016 Environ. Res. 151, 251; Ullah 2016 Chemosphere 152, 35 ...
DOI:
10.1071/RDv32n2Ab179