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You searched for: Journal name Reproduction in domestic animals Remove constraint Journal name: Reproduction in domestic animals Subject term cats Remove constraint Subject term: cats
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1. Key aspects of domestic cat spermatogenesis

Author:
Cristina Gobello
Source:
Reproduction in domestic animals 2022 v.57 no.5 pp. 459-464
ISSN:
0936-6768
Subject:
cats; contraception; meiosis; spermatids; spermatocytes; spermatogonia; spermiogenesis
Abstract:
... In mammals, spermatogenesis is a complex and cyclic process in which a spermatogonia turns into a highly differentiated cell: the spermatozoa. Spermatogenesis comprises proliferation of spermatogonia (spermatocytogenesis), meiosis of spermatocytes and finally differentiation of spermatids into spermatozoa (spermiogenesis). This review summarizes the current knowledge on domestic cat spermatogenesi ...
DOI:
10.1111/rda.14089

2. Expression of oxytocin receptors and oxytocin assisted electroejaculation in the domestic cat (Felis catus)

Author:
Narong Tiptanavattana; Thitida Pakdeesanaeha; Thitiporn Thongsima; Sirichai Techarungchaikul; Theerawat Tharasanit
Source:
Reproduction in domestic animals 2022 v.57 no.5 pp. 489-497
ISSN:
0936-6768
Subject:
cats; epididymis; epithelium; immunohistochemistry; males; oxytocin; peptides; prostaglandin-I synthase; quantitative polymerase chain reaction; smooth muscle; sperm concentration; sperm quality; steroidogenesis; testes; viability
Abstract:
... Oxytocin is a peptide hormone that mainly functions to control the contractility of smooth muscles and sex‐related steroidogenesis in male reproductive tracts. However, specific information concerning this hormone in controlling the reproductive organs of cats is limited. This study aimed to investigate the expression of oxytocin receptors (OTRs) and their signal mediator via prostacyclin synthase ...
DOI:
10.1111/rda.14085

3. Effects of slow freezing and vitrification on embryo development in domestic cat

Author:
Valentina I. Mokrousova; Konstantin A. Okotrub; Eugeny Y. Brusentsev; Elena A. Kizilova; Nikolai V. Surovtsev; Sergei Y. Amstislavsky
Source:
Reproduction in domestic animals 2020 v.55 no.10 pp. 1328-1336
ISSN:
0936-6768
Subject:
Felidae; blastocyst; cats; cryopreservation; cryoprotectants; dimethyl sulfoxide; embryogenesis; freeze-thaw cycles; genetic variation; lipids; oocytes; propylene glycol; reproduction; temperature; vitrification
Abstract:
... Cryopreservation of gametes and embryos is used to maintain genetic diversity of domestic and wild felids. However, felid oocytes and preimplantation embryos contain large amount of intracellular lipids, which affect their cryosensitivity. The objective was to compare the effects of slow freezing and vitrification and to study lipid phase transition (LPT) during cooling in cat embryos. In vitro‐de ...
DOI:
10.1111/rda.13776

4. Granulosa cells in three‐dimensional culture: A follicle‐like structure for domestic cat vitrified oocytes

Author:
Martina Colombo; Maria Giorgia Morselli; Maricy Apparicio; Gaia Cecilia Luvoni
Source:
Reproduction in domestic animals 2020 v.55 Suppl S2 pp. 74-80
ISSN:
0936-6768
Subject:
Felis silvestris; alginates; barium; cats; conspecificity; estradiol; females; meiosis; oocytes; progesterone; reproduction
Abstract:
... Follicle‐like structures are three‐dimensional matrices joint with living cells that allow the in vitro development of female gametes in more physiological conditions. They have been shown to be beneficial to fresh oocytes in different species, and in this study, domestic cat (Felis silvestris catus) granulosa cells were used to create a functional follicle‐like structure aimed at supporting the i ...
DOI:
10.1111/rda.13597

5. Assisted reproduction for felid species conservation—Sperm competences at risk

Author:
Karin Müller; Susanne Eder; Ulrike Jakop; Jürgen Schiller; Peter Müller; Mohammad Bashawat
Source:
Reproduction in domestic animals 2020 v.55 Suppl S2 pp. 55-60
ISSN:
0936-6768
Subject:
Felidae; artificial insemination; cats; cryopreservation; epididymis; female genitalia; insemination; longevity; males; risk; spermatozoa; threatened species
Abstract:
... Cryobanking of gametes in combination with artificial insemination is an essential option to support conservation programmes for endangered and threatened species. About two‐thirds of the felid species are classified as ‘near threatened’, ‘vulnerable’ or ‘endangered’ (www.cites.org), and mostly, epididymal sperm are collected from euthanized or castrated male felids and cryopreserved. However, epi ...
DOI:
10.1111/rda.13581

6. Morphological and morphometric characterization of domestic cat epididymal sperm

Author:
Brenna de Sousa Barbosa; Herlon Victor Rodrigues Silva; Beatriz Evaristo de Almeida Tabosa; Thalles Gothardo Pereira Nunes; Francisco Felipe de Magalhães; Lúcia Daniel Machado da Silva
Source:
Reproduction in domestic animals 2019 v.54 no.12 pp. 1630-1636
ISSN:
0936-6768
Subject:
cats; epididymis; morphometry; spermatozoa
Abstract:
... Sperm morphometry is the tool that confers objectivity to the morphological evaluation by accurately measuring the dimensions of the gamete and its structures. Thus, the aim of the study was to perform a morphometric characterization of the domestic cat sperm. Therefore, sperm samples were collected from twenty pairs of epididymis in a TRIS extender at 37ºC. An aliquot of the sample was used to ma ...
DOI:
10.1111/rda.13572

7. Survivability and developmental competences of domestic cat (Felis catus) oocytes after Cryotech method vitrification

Author:
Agnieszka Nowak; Joanna Kochan; Ewa Świętek; Barbara Kij; Sylwia Prochowska; Wojciech Witarski; Monika Bugno‐Poniewierska; Wojciech Niżański
Source:
Reproduction in domestic animals 2020 v.55 no.8 pp. 992-997
ISSN:
0936-6768
Subject:
assisted reproductive technologies; cats; extinction; humans; liquid nitrogen; models; oocytes; parthenogenesis; toxicity; viability; viability assays; vitrification
Abstract:
... The aim of this study was to evaluate the applicability of the Cryotech technique for the vitrification of domestic cat (Felis catus) oocytes, as a model for other feline species threatened with extinction. This technique, in which oocytes are stored in a minimal volume of medium, is already widely used in human assisted reproductive technology. In the first part of this study, a viability test (E ...
DOI:
10.1111/rda.13741

8. Spermatic and oxidative profile of domestic cat (Felis catus) epididymal sperm subjected to different cooling times (24, 48 and 72 hours)

Author:
DSR Angrimani; KK Nagai; BR Rui; LC Bicudo; JDA Losano; MM Brito; MCP Francischini; M Nichi
Source:
Reproduction in domestic animals 2018 v.53 no.1 pp. 163-170
ISSN:
0936-6768
Subject:
DNA; Felidae; acrosome; antioxidants; cats; cold stress; cooling; cryopreservation; epididymis; glutathione peroxidase; lipid peroxidation; mitochondria; models; oxidation; oxidative stress; plasma membrane; sperm motility; storage temperature; superoxide dismutase; thiobarbituric acid-reactive substances; toluidine blue
Abstract:
... Cooling stored epididymal samples for several days allows facilities to transport and process genetic material post‐mortem. Improvements to this practice allow the preservation of sperm from domestic cats, which are the ideal study model for wild felids. However, the modifications in spermatic features and the oxidative profile are not fully understood in cats. This information is necessary for th ...
DOI:
10.1111/rda.13086

9. The use of human and bovine commercial media for oocyte maturation and embryo development in the domestic cat (Felis catus)

Author:
Sylwia Prochowska; Wojciech Nizanski; Agnieszka Partyka; Joanna Kochan; Wiesława Młodawska; Agnieszka Nowak; Józef Skotnicki; Teresa Grega; Marcin Pałys
Source:
Reproduction in domestic animals 2019 v.54 no.4 pp. 719-726
ISSN:
0936-6768
Subject:
apoptosis; blastocyst; blastomeres; cats; cattle; culture media; embryo culture; embryogenesis; humans; oocytes; variance; viability
Abstract:
... The aim of this study was to examine the suitability of commercial media designed for humans and cattle for oocyte maturation and embryo culture in the domestic cat. In Exp. I, feline oocytes collected ex vivo were subjected to in vitro maturation in a laboratory‐made culture medium (based on M199) or a commercial medium designed for cattle cells (BO‐IVM®). In Exp. II, ICSI‐derived feline embryos ...
DOI:
10.1111/rda.13418

10. Uterine two‐dimensional and Doppler ultrasonographic evaluation of feline pyometra

Author:
Paula Graciela Blanco; Ana Rube; Mariana López Merlo; Pablo Rodrigo Batista; Sol Arioni; Ignacio López Knudsen; Mariana Tórtora; Cristina Gobello
Source:
Reproduction in domestic animals 2018 v.53 Suppl S3 pp. 70-73
ISSN:
0936-6768
Subject:
cats; parturition; pregnancy; pyometra; signs and symptoms (animals and humans); surgery; ultrasonography; uterus
Abstract:
... The aim of this study was to compare uterine two‐dimensional and Doppler ultrasonographic parameters in queens suffering from pyometra from those in early pregnancy. Secondly, the effect of the presence of clinical signs of systemic illness on these parameters was also described. Fourteen post‐oestrous queens, with uterine luminal content in the absence of embryos were included. According to their ...
DOI:
10.1111/rda.13324

11. A comparison of quality parameters of fresh feline ejaculates collected by three different collection techniques

Author:
Klara Jelinkova; Roman Vitasek; Robert Novotny; Alena Bartoskova
Source:
Reproduction in domestic animals 2018 v.53 no.5 pp. 1068-1074
ISSN:
0936-6768
Subject:
adults; anesthesia; castration; catheters; cats; epididymis; head; males; medetomidine; semen; slicing; sperm concentration; sperm quality; viability
Abstract:
... The aim of our study was to compare the quality parameters of fresh feline ejaculates collected by three different techniques—urethral catheterization after medetomidine administration (CT), electroejaculation (EE) and epididymal slicing after orchiectomy (EP). A total of 34 adult male cats (Felis catus) were included in the study. In all male cats, the sperm collection was performed under general ...
DOI:
10.1111/rda.13205

12. Analysis of gene expression in granulosa cells post‐maturation to evaluate oocyte culture systems in the domestic cat

Author:
J Zahmel; H Mundt; K Jewgenow; BC Braun
Source:
Reproduction in domestic animals 2017 v.52 Suppl S2 no. pp. 65-70
ISSN:
0936-6768
Subject:
additives; anti-Mullerian hormone; aromatase; cats; embryogenesis; follicle-stimulating hormone; follicle-stimulating hormone receptors; gene expression; genes; granulosa cells; humans; in vitro fertilization; luteinization; luteinizing hormone; messenger RNA; oocytes; prostaglandins; quantitative polymerase chain reaction; swine
Abstract:
... Maturation of oocytes is a prerequisite for successful embryo development. The fertilization competence of in vivo derived oocytes is significantly higher than that of oocytes matured in vitro. Commonly evaluated morphological criteria for oocyte maturation do not reflect the complexity and quality of maturation processes. Oocytes and granulosa cells are communicating closely in a bidirectional wa ...
DOI:
10.1111/rda.12927

13. Cell cycle synchronization and analysis of apoptosis‐related gene in skin fibroblasts from domestic cat (Felis silvestris catus) and kodkod (Leopardus guigna)

Author:
D Veraguas; PF Gallegos; FO Castro; L Rodriguez‐Alvarez
Source:
Reproduction in domestic animals 2017 v.52 no.5 pp. 881-889
ISSN:
0936-6768
Subject:
Felis; Leopardus; blood serum; cats; cell cycle; differential staining; fibroblasts; flow cytometry; gene expression; genes; somatic cells; starvation; viability
Abstract:
... The kodkod population is in constant decrease and the somatic cell nuclear transfer (SCNT) might help to preserve the genetic pool of this species. The cell cycle synchronization of donor cells plays a crucial role in SCNT. The objective of this research was to evaluate two different methods for quiescence induction, serum starvation (SS) and contact inhibition (CI), both for 1, 3 and 5 days, on s ...
DOI:
10.1111/rda.12994

14. Detection of Simonsiella spp. in the Vagina of Lions and Leopard in Oestrus

Author:
Isabel Callealta; Andre Ganswindt; Sara Goncalves; Abraham Mathew; Imke Lueders
Source:
Reproduction in domestic animals 2018 v.53 no.6 pp. 1605-1608
ISSN:
0936-6768
Subject:
Escherichia coli; Felidae; Gram-negative bacteria; Simonsiella; Streptococcus canis; aerobes; cats; coagulase negative staphylococci; estrus; females; flora; lips; monitoring; reproductive behavior; respiratory system; vagina; zoos
Abstract:
... Reports of the vaginal flora of wild cats such as lions or leopards are scarce. The micro‐organisms most commonly found in the vagina of clinically healthy cats are aerobic bacteria such as coagulase‐negative Staphylococcus, Streptococcus canis, and Escherichia coli. Simonsiella spp are large Gram‐negative bacteria belonging to the Neisseriaceae family, typically found in the oral cavity and upper ...
DOI:
10.1111/rda.13298

15. Disorder of sex development in a cat with chromosome mosaicism 37,X/38,X,r(Y)

Author:
I Szczerbal; M Stachowiak; J Nowacka‐Woszuk; S Dzimira; K Szczepanska; M Switonski
Source:
Reproduction in domestic animals 2017 v.52 no.5 pp. 914-917
ISSN:
0936-6768
Subject:
X chromosome; abnormal development; cats; cytogenetic analysis; fluorescence in situ hybridization; genes; genitalia; histopathology; metaphase; penis; scrotum; telomeres; testes; uterus
Abstract:
... An 18‐month‐old European shorthair cat was subjected to genetic studies due to ambiguous external genitalia (underdeveloped both penis and scrotum). Further anatomic and histopathological studies revealed the presence of abdominal, atrophic testes and uterus. Cytogenetic analysis showed two cell lines, one with X monosomy—37,X [90% of the analysed metaphase spreads], and other line had 38 chromoso ...
DOI:
10.1111/rda.12968

16. Disorders of sex development in cats with different complements of sex chromosomes

Author:
Izabela Szczerbal; Paulina Krzeminska; Stanislaw Dzimira; Tuire Maria Tamminen; Seppo Saari; Wojciech Nizanski; Maciej Gogulski; Joanna Nowacka‐Woszuk; Marek Switonski
Source:
Reproduction in domestic animals 2018 v.53 no.6 pp. 1317-1322
ISSN:
0936-6768
Subject:
DNA; Y chromosome; cats; chimerism; chromosome number; genes; genetic background; hair follicles; males; metabolism; mutation; patients; penis; phenotype; polymerase chain reaction; scrotum; steroid hormones; testes; testosterone; trisomics; uterus
Abstract:
... The genetic background of disorders of sex development (DSDs) in cats is poorly understood, due to a relatively low number of such studies in this species. Here we present three new DSD cases with different complements of sex chromosomes. The first, an Oriental Shorthair cat with a rudimentary penis, abdominal atrophic testicles and lack of uterus appeared to be a freemartin, since leucocyte chime ...
DOI:
10.1111/rda.13263

17. Ethics in animal breeding

Author:
Wenche Farstad
Source:
Reproduction in domestic animals 2018 v.53 Suppl S3 pp. 4-13
ISSN:
0936-6768
Subject:
animal breeding; animal welfare; cats; dairy cows; dogs; ethics; ewes; food production; food safety; genetic resources; horses; income; meat; milk yield; pets; progeny; sows; veterinarians
Abstract:
... Ethical breeding involves the use of healthy animals true to their species in behaviour and physical appearance, and when applicable, showing a sustainable performance. The concerns for the species/breed are essential parts of the breeding goals, including preservation of genetic resources within the species/breed, and the health and welfare of the individual animal. Ethical and welfare considerat ...
DOI:
10.1111/rda.13335

18. Retained fertilizing capability in cryopreserved feline spermatozoa

Author:
K Chatdarong
Source:
Reproduction in domestic animals 2017 v.52 Suppl S2 no. pp. 261-264
ISSN:
0936-6768
Subject:
DNA; Felis chaus; adenosine triphosphate; anesthesia; blastocyst; cats; centrifugation; chromatin; cold storage; conception rate; cryopreservation; epididymis; freezing; insemination; intracytoplasmic sperm injection; males; morula; progeny; sperm quality; temperature; testes; thawing
Abstract:
... Sperm cryopreservation offers a long‐term preservation of male genetic materials for future assisted reproductive technologies. However, dramatic changes in temperature during freezing and thawing injure sperm cells. While motility is essential for AI and membrane integrity is crucial for in vitro fertilization (IVF), sperm DNA integrity is a common index of fertilizing capability required for AI, ...
DOI:
10.1111/rda.12855

19. Safety and effectiveness of a single and repeat intramuscular injection of a GnRH vaccine (GonaCon™) in adult female domestic cats

Author:
LM Vansandt; MA Kutzler; AE Fischer; KN Morris; WF Swanson
Source:
Reproduction in domestic animals 2017 v.52 Suppl S2 no. pp. 348-353
ISSN:
0936-6768
Subject:
adults; antibodies; antigens; cats; contraception; environmental protection; females; gonadotropin-releasing hormone; humoral immunity; immunization; intramuscular injection; risk; vaccines; wildlife; United States
Abstract:
... Sterilization is a key strategy to reduce the number of domestic cats entering and killed in shelters each year. However, surgical sterilization is expensive and labour‐intensive and cannot fully address the 70 million free‐roaming cats estimated to exist in the United States. GonaCon™ is a gonadotropin‐releasing hormone vaccine originally developed for use as a wildlife immunocontraceptive. An ea ...
DOI:
10.1111/rda.12853

20. Sperm cryopreservation in the Far‐Eastern wildcat (Prionailurus bengalensis euptilurus)

Author:
Sergei Amstislavsky; Eugeny Brusentsev; Elena Kizilova; Valentina Mokrousova; Valeria Kozhevnikova; Tatyana Abramova; Irina Rozhkova; Sergey Naidenko
Source:
Reproduction in domestic animals 2018 v.53 no.5 pp. 1219-1226
ISSN:
0936-6768
Subject:
Prionailurus bengalensis euptilurus; adults; cats; confocal laser scanning microscopy; cryopreservation; epididymis; flagellum; freeze-thaw cycles; freezing; light microscopy; males; oocytes; spermatozoa; staining; thawing; viability
Abstract:
... The Far‐Eastern wildcat (Prionailurus bengalensis euptilurus) is a rare and poorly investigated nondomestic felid species. An attempt of freezing and cryopreserving Far‐Eastern wildcat spermatozoa in CaniPlus Freeze (CPF) medium is reported. Sperm was collected by electroejaculation from five adult Far‐Eastern wildcat captive‐born males. Epididymal spermatozoa from five adult randomly bred domesti ...
DOI:
10.1111/rda.13230

21. The effect of cumulus cells on domestic cat (Felis catus) oocytes during in vitro maturation and fertilization

Author:
N Sowińska; K Frankowska; A Filipczyk; A Adamaszek; K Nalik; K Fic; A Pietsch‐Fulbiszewska
Source:
Reproduction in domestic animals 2017 v.52 Suppl S2 no. pp. 108-113
ISSN:
0936-6768
Subject:
cats; coculture; fluorescence microscopes; in vitro fertilization; meiosis; morula; oocytes; ovariectomy
Abstract:
... The aim of this study was to evaluate the effect of co‐culture of denuded oocytes with cumulus cells (CC) or cumulus–oocyte complexes (COCs) on in vitro maturation (IVM) and in vitro fertilization (IVF). Immature oocytes were collected from ovaries of domestic cats following a routine ovariectomy. Oocytes were matured in vitro for 24 hr within four groups: (i) denuded oocytes (DO), (ii) DO co‐cult ...
DOI:
10.1111/rda.12895

22. The never‐ending search of an ideal culture system for domestic cat oocytes and embryos

Author:
Gaia Cecilia Luvoni; Martina Colombo; Maria Giorgia Morselli
Source:
Reproduction in domestic animals 2018 v.53 Suppl S3 pp. 110-116
ISSN:
0936-6768
Subject:
cats; chemistry; coculture; in vitro fertilization; oocytes; organ-on-a-chip; oviductal fluids; oviducts; physics
Abstract:
... In the domestic cat, in vitro fertilization started 40 years ago, but an ideal culture system has yet to be achieved. The physiological microenvironments, which interact with oocytes and embryos promoting their competence, have been investigated. However, recreating in vitro follicle‐ and oviduct‐like conditions is challenging and a matter of both chemistry and physics. This review presents an exc ...
DOI:
10.1111/rda.13331

23. Transplantation of amniotic membrane‐derived multipotent cells ameliorates and delays the progression of chronic kidney disease in cats

Author:
AS Vidane; AO Pinheiro; JB Casals; D Passarelli; MCFNS Hage; RS Bueno; DS Martins; CE Ambrósio
Source:
Reproduction in domestic animals 2017 v.52 Suppl S2 no. pp. 316-326
ISSN:
0936-6768
Subject:
amnion; anesthesia; animal models; blood serum; cats; creatinine; disease course; fibrosis; hematuria; inflammation; intravenous injection; kidney diseases; kidneys; renal function; renoprotective effect; sedation; specific gravity; stem cells; urine
Abstract:
... Chronic kidney disease (CKD) is a common clinical condition in domestic cats, characterized by tubulointerstitial, vascular and glomerular inflammation and severe fibrosis. Studies in rodent model of induced CKD have shown a decrease and stabilization of the clinical condition. In this study was evaluated the safety and effect of intrarenal and intravenous infusion of allogeneic mesenchymal stem c ...
DOI:
10.1111/rda.12846

24. Ultrasonographic evaluation of ovarian morphology and blood flow in prepubertal and pubertal cats

Author:
Rosario Vercellini; Cristina Gobello; Florencia D'Francisco; Silvia Olguín; Analía Arizmendi; Raúl Rodríguez; Daniel Osvaldo Arias; Paula G. Blanco
Source:
Reproduction in domestic animals 2018 v.53 Suppl S3 pp. 74-78
ISSN:
0936-6768
Subject:
arteries; blood flow; cats; females; puberty; ultrasonography
Abstract:
... The aim of this study was to evaluate two‐dimensional and Doppler ultrasonographic changes of feline ovaries before and during puberty. Nine, 3‐month‐old female cats were followed until puberty (Day 1). Two‐dimensional and Doppler ultrasound evaluations of the ovaries were carried out on Days ‐50, ‐20, ‐7 and 1. Longitudinal and transverse sections of the ovaries were measured and all anechoic sph ...
DOI:
10.1111/rda.13355

25. Use of the gonadotropin‐releasing hormone (GnRH) stimulation test to monitor gonadal function in intact adult male cats

Author:
S Romagnoli; A Baldan; C Righetti; C Fontaine; L Scenna; T Badon; C Stelletta; C Milani; M Cecchetto; A Mollo
Source:
Reproduction in domestic animals 2017 v.52 no.1 pp. 24-27
ISSN:
0936-6768
Subject:
adults; adverse effects; blood sampling; blood serum; cats; dogs; gonadotropin-releasing hormone; gonads; intravenous injection; males; testosterone
Abstract:
... The gonadotropin‐releasing hormone (GnRH) stimulation test is a common procedure used to investigate normality of the pituitary‐gonadal axis in mammals. There is very little information on the technique, its efficacy and side effects in small animals and in particular no information for male cats. In dogs, such test is performed by intravenous (IV) administration. With cats, the number of times th ...
DOI:
10.1111/rda.12771

26. eCG stimulation in domestic cats increases the expression of gonadotrophin‐induced genes improving oocyte competence during the non‐breeding season

Author:
Daniel Veraguas; Sandra R. Cuevas; Paula F. Gallegos; Darling Saez‐Ruiz; Fidel O. Castro; Lleretny Rodriguez‐Alvarez
Source:
Reproduction in domestic animals 2018 v.53 no.6 pp. 1306-1316
ISSN:
0936-6768
Subject:
blastocyst; breeding season; cats; embryogenesis; equine chorionic gonadotropin; gene expression; genes; human chorionic gonadotropin; oocytes; parthenogenesis
Abstract:
... The objective of this study was to evaluate the eCG stimulation on domestic cat oocyte competence during the non‐breeding season. Four experimental groups were made: (a) untreated cycling cats (Breeding season group; BS), (b) untreated anestrous cats (Non‐breeding group; NB), (c) anestrous cats treated with 200 IU of eCG (eCG group) and (d) anestrous cats treated with 200 IU of eCG and 100 IU of h ...
DOI:
10.1111/rda.13229

27. A three‐dimensional alginate system for in vitro culture of cumulus‐denuded feline oocytes

Author:
MG Morselli; S Canziani; D Vigo; GC Luvoni
Source:
Reproduction in domestic animals 2017 v.52 no.1 pp. 83-88
ISSN:
0936-6768
Subject:
alginates; barium; cats; coculture; meiosis; oocytes; viability
Abstract:
... In the case of high valuable individuals with very precious genetic material, widening the genetic pool including gametes with poor morphological characteristics, as cumulus‐denuded oocytes (CDOs), could be an option. To improve the in vitro culture of low‐competence feline CDOs, an enriched three‐dimensional (3D) system in association with competent cumulus–oocyte complexes (COCs) was developed. ...
DOI:
10.1111/rda.12806

28. Age and anti‐Müllerian hormone levels predict the success of in vitro maturation of cat oocytes

Author:
F Snoeck; S Sarrazin; E Wydooghe; A Van Soom
Source:
Reproduction in domestic animals 2017 v.52 Suppl S2 no. pp. 98-102
ISSN:
0936-6768
Subject:
anti-Mullerian hormone; blood; cats; oocytes; probability; puberty
Abstract:
... Up to date, in vitro maturation (IVM) rates of oocytes are highly variable between individual cats. This study was carried out to investigate the predictive value of age and anti‐Müllerian hormone (AMH) concentration in relation to capacity for IVM of cat oocytes. Ovaries were collected from 33 cats, which were divided into three age groups: (i) 0–3 months (pre‐pubertal); (ii) 3–12 months (peripub ...
DOI:
10.1111/rda.12827

29. Beneficial effect of extracellular adenosine 5′‐triphosphate treatment on the Indochinese leopard (Panthera pardus delacouri) sperm quality after cryopreservation

Author:
P Thuwanut; W Tipkantha; B Siriaroonrat; P Comizzoli; K Chatdarong
Source:
Reproduction in domestic animals 2017 v.52 Suppl S2 no. pp. 269-274
ISSN:
0936-6768
Subject:
Convention on International Trade in Endangered Species; Panthera pardus; adenosine; adenosine triphosphate; adults; artificial insemination; breeding programs; cats; cryopreservation; ejaculation; embryogenesis; in vitro fertilization; males; membrane potential; mitochondrial membrane; oocytes; plasma membrane; semen; sperm motility; sperm quality; straw; thawing
Abstract:
... The Indochinese leopard (Panthera pardus delacouri) population, included in CITES Appendix I, has been declining for decades. Proper gamete preservation condition is critical for breeding programme management using artificial insemination or in vitro fertilization (IVF). The present study aimed at investigating the impact of post‐thawing treatment of leopard semen with extracellular adenosine 5′‐t ...
DOI:
10.1111/rda.12854

30. Changes in c‐erbB‐2 Immunoexpression in Feline Endometrial Adenocarcinomas

Author:
AL Saraiva; R Payan‐Carreira; F Gärtner; F Faria; LM Lourenço; MA Pires
Source:
Reproduction in domestic animals 2016 v.51 no.1 pp. 33-39
ISSN:
0936-6768
Subject:
adenocarcinoma; antibodies; carcinogenesis; cats; endometrium; epidermal growth factor receptors; epithelial cells; epithelium; erbB-2 receptor; estrous cycle; immunohistochemistry; oncogene proteins
Abstract:
... Human epidermal growth factor receptor type 2 (c‐erbB‐2), an oncoprotein with potential prognostic marker and therapeutic use, is overexpressed in several human and animal tumours. But information regarding this molecule in feline tumours is scarce. This study aimed to assess the changes in the immunohistochemical expression of c‐erbB‐2 in feline endometrial adenocarcinomas (FEA) compared to norma ...
DOI:
10.1111/rda.12642

31. Characterization of teratogenic potential and gene expression in canine and feline amniotic membrane‐derived stem cells

Author:
MT Cardoso; AO Pinheiro; AS Vidane; JB Casals; VC de Oliveira; NJN Gonçalves; DS Martins; CE Ambrósio
Source:
Reproduction in domestic animals 2017 v.52 Suppl S2 no. pp. 58-64
ISSN:
0936-6768
Subject:
biosafety; cats; cell transplantation; dogs; flow cytometry; gene expression; humans; medicine; mice; neoplasms; quantitative polymerase chain reaction; stem cells; teratogenicity
Abstract:
... The biosafety of innovative procedures that utilize stem cells in regenerative medicine has been addressed in several studies. Previous work has showed no tumour formation following the use of feline and human amniotic membrane‐derived stem cells (AMSCs). In contrast, tumour formation was observed when canine AMSCs were utilized. These findings suggested that feline and human, but not canine, AMSC ...
DOI:
10.1111/rda.12832

32. Comparison of the ovarian and uterine reproductive parameters, and the ovarian mRNA and protein expression of LHR and FSHR between the prepubertal and adult female cats

Author:
NS Mehl; M Khalid; S Srisuwatanasagul; T Swangchan‐Uthai; S Sirivaidyapong
Source:
Reproduction in domestic animals 2017 v.52 Suppl S2 no. pp. 41-44
ISSN:
0936-6768
Subject:
adults; cats; endometrium; estrous cycle; females; messenger RNA; ovarian follicles; protein synthesis; puberty; quantitative polymerase chain reaction
Abstract:
... This study aimed to evaluate and compare the ovarian and uterine characteristics along with the ovarian mRNA and protein expression of LHR and FSHR between the pre‐pubertal and adult female cats. The uterine horns and ovaries were collected from pre‐pubertal and adult female cats at their follicular, luteal and interoestrous stages of the oestrous cycle (n = 6/group). Endometrial and myometrial th ...
DOI:
10.1111/rda.12926

33. Cryopreservation of feline oocytes by vitrification using commercial kits and slush nitrogen technique

Author:
L Fernandez‐Gonzalez; K Jewgenow
Source:
Reproduction in domestic animals 2017 v.52 Suppl S2 no. pp. 230-234
ISSN:
0936-6768
Subject:
analytical kits; assisted reproductive technologies; cats; cryopreservation; dimethyl sulfoxide; endangered species; ethylene glycol; fetal bovine serum; ficoll; freezing; genetic variation; immatures; morula; nitrogen; oocytes; slicing; survival rate; trehalose; vitrification
Abstract:
... Assisted reproductive techniques are a valuable tool for conservation breeding of endangered species. Cryopreservation methods are the basis of gamete banks, supporting genetic diversity preservation. Unfortunately, cryopreservation of feline oocytes is still considered an experimental technique. The aim of this study was to compare two commercial kits, with our protocol for vitrification of cat o ...
DOI:
10.1111/rda.12837

34. Deciphering the mechanisms involving cenexin, ninein and centriolin in sperm maturation during epididymal transit in the domestic cat

Author:
T Rowlison; MA Ottinger; P Comizzoli
Source:
Reproduction in domestic animals 2017 v.52 Suppl S2 no. pp. 193-196
ISSN:
0936-6768
Subject:
adults; antibodies; aster (cell structure); cats; centrosomes; epididymis; fertilization (reproduction); fluorescent antibody technique; in vitro culture; males; mitotic spindle apparatus; rete testis; scaffolding proteins; spermatozoa
Abstract:
... The sperm centrosome is an essential organelle with a key role in organizing the sperm aster for proper syngamy and formation of the first mitotic spindle. The sperm cell acquires the functional capability during epididymal transit by incorporation of key factors. The objective of the study was to identify these key maturation proteins, such as ninein and centriolin as well as cenexin—a scaffold p ...
DOI:
10.1111/rda.12831

35. Diagnostic possibilities from a serum sample—Clinical value of new methods within small animal reproduction, with focus on anti‐Müllerian hormone

Author:
BS Holst
Source:
Reproduction in domestic animals 2017 v.52 Suppl S2 no. pp. 303-309
ISSN:
0936-6768
Subject:
Sertoli cells; animal reproduction; anti-Mullerian hormone; blood serum; cats; dogs; esterases; granulosa cells; hyperplasia; liquid chromatography; males; steroids; tandem mass spectrometry; veterinarians
Abstract:
... During the last decade, analysis of anti‐Müllerian hormone (AMH), highly conserved between mammalian species, has contributed to new information in reproductive endocrinology, due to clinically available diagnostic assays. AMH is produced solely in the gonads, in the Sertoli cells of testes and granulosa cells of the ovary, and thus offers possibilities to diagnose physiologic and pathologic condi ...
DOI:
10.1111/rda.12856

36. Different associations of cryoprotectants for testicular tissue of prepubertal cats submitted to vitrification

Author:
DBC Lima; TFP Silva; GB Morais; A Aquino‐Cortez; JSAM Evangelista; FAF Xavier Júnior; DA Viana; LDM Silva
Source:
Reproduction in domestic animals 2017 v.52 Suppl S2 no. pp. 235-241
ISSN:
0936-6768
Subject:
biotechnology; cats; cell differentiation; cell proliferation; cryopreservation; cryoprotectants; dimethyl sulfoxide; ethylene glycol; puberty; shrinkage; testes; vitrification
Abstract:
... The cryopreservation of testicular tissue is presented as the only alternative for the preservation of genetic material from prepubertal animals. However, this biotechnology is still being tested. The objective of this study was to evaluate the effect of different associations of cryoprotectants and the potential of cell proliferation after vitrification of testicular tissue of prepubertal cats. F ...
DOI:
10.1111/rda.12833

37. Distribution of mast cells in the feline ovary in various phases of the oestrous cycle

Author:
P Hamouzova; P Cizek; R Novotny; A Bartoskova; F Tichy
Source:
Reproduction in domestic animals 2017 v.52 no.3 pp. 483-486
ISSN:
0936-6768
Subject:
anestrus; blood serum; cats; diestrus; estradiol; estrus; mast cells; ovulation; progesterone
Abstract:
... This study is the first description of the distribution of mast cells in various phases of the oestrous cycle in the ovary of cat. Furthermore, this is the first description in species with an induced ovulation. The aim was to describe the distribution of mast cells and variability of their numbers in the feline ovaries in different phases of the oestrous cycle. The number of mast cells in medulla ...
DOI:
10.1111/rda.12938

38. Engineering a gene silencing viral construct that targets the cat hypothalamus to induce permanent sterility: An update

Author:
GA Dissen; K Adachi; A Lomniczi; T Chatkupt; BL Davidson; H Nakai; SR Ojeda
Source:
Reproduction in domestic animals 2017 v.52 Suppl S2 no. pp. 354-358
ISSN:
0936-6768
Subject:
engineering; gene therapy; RNA interference; Dependoparvovirus; hypothalamus; neurons; reproduction; cats; serotypes; sterilizing; viruses; neutralizing antibodies; tissues
Abstract:
... The intent of this contribution is to provide an update of the progress we have made towards developing a method/treatment to permanently sterilize cats. Our approach employs two complementary methodologies: RNA interference (RNAi) to silence genes involved in the central control of reproduction and a virus‐based gene therapy system intended to deliver RNAi selectively to the hypothalamus (where t ...
DOI:
10.1111/rda.12834
PubMed:
27859771
PubMed Central:
PMC5791761

39. Evaluation of different fragment sizes and cryoprotectants for cryopreservation of feline testicular tissues

Author:
BI Macente; GH Toniollo; M Apparicio; CFM Mansano; HE Thomé; CL Canella; MEG Tozato; RR Gutierrez
Source:
Reproduction in domestic animals 2017 v.52 Suppl S2 no. pp. 242-247
ISSN:
0936-6768
Subject:
analysis of variance; basement membrane; cats; cell nucleolus; cryopreservation; cryoprotectants; egg yolk; freeze-thaw cycles; glycerol; molecular weight; testes; thiobarbituric acid-reactive substances
Abstract:
... This study aimed to evaluate tissue damage of feline testicles sectioned in two different sizes (0.3 or 0.5 cm³) and submitted to different cryoprotectants (propanediol or glycerol). Testicles obtained from 12 domestic cats were sectioned in 0.3 and 0.5 cm³ sized pieces and immediately evaluated by TBARS and semi‐quantitatively by histomorphology. The remaining fragments were placed in cryotubes w ...
DOI:
10.1111/rda.12828

40. Expression of steroidogenic enzymes and steroid receptors in foetal gonads of domestic cat—Sex similarities and differences

Author:
BC Braun; K Jewgenow
Source:
Reproduction in domestic animals 2017 v.52 Suppl S2 no. pp. 130-136
ISSN:
0936-6768
Subject:
aromatase; brain; cats; estrogen receptors; estrogens; females; gene expression; genes; genitalia; gonads; males; messenger RNA; oogenesis; pregnancy; progesterone; progesterone receptors; staining; steroid hormones
Abstract:
... Foetal gonads already produce steroid hormones and by this influence the further development of external and internal genitalia as well as of the brain. Beside this, foetal gonads themselves can be influenced by foetal or maternal hormones. The time course of foetal gonadal development can differ between species. As knowledge on processes in domestic cats is very limited, the steroidogenic enzyme ...
DOI:
10.1111/rda.12829

41. Freeze‐dried spermatozoa: A future tool?

Author:
M Olaciregui; L Gil
Source:
Reproduction in domestic animals 2017 v.52 Suppl S2 no. pp. 248-254
ISSN:
0936-6768
Subject:
DNA; ambient temperature; cats; cryopreservation; dogs; freeze drying; genetic resources; humans; intracytoplasmic sperm injection; nitrogen; oocytes; progeny; refrigerators; spermatozoa
Abstract:
... Cryopreservation has been routinely used to preserve sperm of human and different animal species. However, frozen sperm storage for a long time brings many inconveniences because of liquid nitrogen. Many attempts have been made to overcome the disadvantages of the current cryopreservation method. Freeze‐drying has been proposed as alternative method for sperm preservation to achieve the ability to ...
DOI:
10.1111/rda.12838

42. Long‐term effects of GnRH agonists on fertility and behaviour

Author:
S Goericke‐Pesch
Source:
Reproduction in domestic animals 2017 v.52 Suppl S2 no. pp. 336-347
ISSN:
0936-6768
Subject:
aggression; bitches; castration; cats; deslorelin acetate; estrus; gonadotropin-releasing hormone; humans; libido; long term effects; males; secretion; semen quality; spermatogenesis; steroidogenesis; testosterone; urine
Abstract:
... This review aimed to summarize the present knowledge about the effects of GnRH agonist slow‐release implants (GnRH A‐SRI) on fertility and behaviour in male and female dogs and cats with special focus on deslorelin. Following an initial stimulation of gonadotropin and testosterone secretion possibly associated with an improved semen quality, GnRH A‐SRI induce long‐term depression of fertility in m ...
DOI:
10.1111/rda.12898

43. Matrix‐assisted laser desorption/ionization imaging mass spectrometry for the spatial location of feline oviductal proteins

Author:
M Apparicio; VG Santos; DFO Rocha; CR Ferreira; BI Macente; GM Magalhães; AE Alves; TF Motheo; LC Padilha‐Nakaghi; EA Pires‐Buttler; GC Luvoni; MN Eberlin; WRR Vicente
Source:
Reproduction in domestic animals 2017 v.52 Suppl S2 no. pp. 88-92
ISSN:
0936-6768
Subject:
cats; cell differentiation; cell movement; desorption; embryogenesis; glass; histology; image analysis; indium tin oxide; ionization; ions; keratin; mass spectrometry; nitrogen; oviducts; protein composition; thymosin
Abstract:
... With the purpose of identifying factors involved in early stages of embryo development in the domestic cat, matrix‐assisted laser desorption/ionization imaging mass spectrometry (MALDI‐IMS) was used for the first time to describe the spatial localization of proteins in the oviducts of queens. Oviducts were obtained from two 2 and 4 years old cross‐bred queens, divided into three segments, snap‐fro ...
DOI:
10.1111/rda.12842

44. Metabolism of prostaglandin F2alpha in Eurasian lynx (Lynx lynx) and Asian leopard cat (Prionailurus bengalensis euptilura)

Author:
M Dehnhard; SV Naidenko; K Jewgenow
Source:
Reproduction in domestic animals 2017 v.52 Suppl S2 no. pp. 45-51
ISSN:
0936-6768
Subject:
Lynx lynx; Prionailurus bengalensis; carnivores; cats; feces; high performance liquid chromatography; lungs; metabolism; metabolites; monitoring; pregnancy diagnosis; prostaglandins; radiolabeling; tritium; urine
Abstract:
... Methods for monitoring endocrine status are useful tools for reproduction management. In particular, successful captive breeding of endangered feline species requires reliable methods for pregnancy diagnosis. In many species, uterine and placental prostaglandin‐F2α (PGF2α) is involved in the regulation of reproductive processes. PGF2α is metabolized to 13,14‐dihydro‐15‐keto‐PGF2a (PGFM) during the ...
DOI:
10.1111/rda.12894

45. Mitochondrial characteristics in oocytes of the domestic cat (Felis catus) after in vitro maturation and vitrification

Author:
N Sowińska; K Müller; W Niżański; K Jewgenow
Source:
Reproduction in domestic animals 2017 v.52 no.5 pp. 806-813
ISSN:
0936-6768
Subject:
cats; confocal microscopy; fluorescence; germinal vesicle; metaphase; mitochondria; staining; vitrification
Abstract:
... CONTENTS: The objective of this study was to evaluate mitochondria in immature and in vitro‐matured domestic cat oocytes and to assess for the first time the effect of vitrification on mitochondrial traits. Mitochondrial distribution and aggregation were assessed using confocal microscopy after staining with the fluorescent dye—MitoTracker® Red CMXRos. Only cells at the germinal vesicle and the me ...
DOI:
10.1111/rda.12982

46. Molecular markers of putative spermatogonial stem cells in the domestic cat

Author:
SJ Bedford‐Guaus; S Kim; L Mulero; JM Vaquero; C Morera; R Adan‐Milanès; A Veiga; Á Raya
Source:
Reproduction in domestic animals 2017 v.52 Suppl S2 no. pp. 177-186
ISSN:
0936-6768
Subject:
Felidae; adults; antibodies; assisted reproductive technologies; cats; cell culture; culture media; epithelial cell adhesion molecule; flow cytometry; gene expression; genetic markers; immunohistochemistry; leukemia; mice; models; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; spermatogonia; stem cells; surface proteins; testes; transcription (genetics); transcription factors; zinc finger motif
Abstract:
... Spermatogonial stem cells (SSCs) are an important tool for fertility preservation and species conservation. The ability to expand SSCs by in vitro culture is a crucial premise for their use in assisted reproduction. Because SSCs represent a small proportion of the germ cells in the adult testis, culture success is aided by pre‐enrichment through sorting techniques based on cell surface‐specific ma ...
DOI:
10.1111/rda.12819

47. New approaches to non‐surgical sterilization for dogs and cats: Opportunities and challenges

Author:
Linda Rhodes
Source:
Reproduction in domestic animals 2017 v.52 Suppl S2 no. pp. 327-331
ISSN:
0936-6768
Subject:
cats; cytotoxins; deslorelin acetate; dogs; euthanasia; females; feral animals; gene therapy; gonadotropin-releasing hormone; grants; hypothalamus; immunity; immunocontraception; males; models; neurons; pets; small interfering RNA; United States
Abstract:
... Over the last 40 years, researchers have explored methods to non‐surgically suppress fertility in animals. Immunocontraception has been used to control wildlife populations but does not confer long‐term immunity. The gonadotropin‐releasing hormone (GnRH) agonist deslorelin, formulated as an implant to provide 6‐month to 1‐year suppression of fertility in male dogs, is available commercially in som ...
DOI:
10.1111/rda.12862

48. Presence of sucrose in the vitrification solution and exposure for longer periods of time improve post‐warming follicle integrity in cat ovarian tissues

Author:
L Mouttham; P Comizzoli
Source:
Reproduction in domestic animals 2017 v.52 Suppl S2 no. pp. 224-229
ISSN:
0936-6768
Subject:
cats; cortex; cryopreservation; endangered species; exposure duration; histology; models; nitrogen; sucrose; temperature; thawing; tissue culture; vitrification
Abstract:
... Ovarian tissue cryopreservation followed by tissue culture is a promising approach to preserving the fertility of biomedical models and endangered species. The objective of this study was to investigate the impact of exposure time to vitrification solution and presence of sucrose using different exposure temperatures and base media on intra‐ovarian follicle integrity. Peripubertal ovarian cortical ...
DOI:
10.1111/rda.12847

49. Research on reproduction is essential for captive breeding of endangered carnivore species

Author:
K Jewgenow; BC Braun; M Dehnhard; J Zahmel; F Goeritz
Source:
Reproduction in domestic animals 2017 v.52 Suppl S2 no. pp. 18-23
ISSN:
0936-6768
Subject:
assisted reproductive technologies; breeding programs; carnivores; cats; contraception; cryopreservation; dogs; embryogenesis; endangered species; females; ferrets; genetically modified organisms; germ cells; models; periodicity; pets; reproductive traits
Abstract:
... Assisted reproductive technology (ART) has great potential for conservation, but its successful application in captive breeding programmes of endangered species is often compromised by limited background on species' biology. Although carnivore species benefit from knowledge obtained in domesticated species (dogs, cats and ferrets), the focus of research is different. In pet animals, research in re ...
DOI:
10.1111/rda.12836

50. Survival rate after vitrification of various stages of cat embryos and blastocyst with and without artificially collapsed blastocoel cavity

Author:
M Ochota; B Wojtasik; W Niżański
Source:
Reproduction in domestic animals 2017 v.52 Suppl S2 no. pp. 281-287
ISSN:
0936-6768
Subject:
blastocyst; blastomeres; cats; cryopreservation; developmental stages; ice; shrinkage; survival rate; vitrification
Abstract:
... Embryo vitrification is a modern technique for cryopreservation in assisted reproductive programs. From all the embryos, blastocysts are the most challenging during cryostorage due to their size, multicellular structure and the presence of blastocoelic fluid. The aim of this study was to evaluate the suitability for vitrification of various developmental stages of feline embryos and the influence ...
DOI:
10.1111/rda.12826

51. Temporal changes in serum luteinizing hormone following ovariohysterectomy and gonadotropin‐releasing hormone vaccination in domestic cats

Author:
HL Bateman; LM Vansandt; J Newsom; WF Swanson
Source:
Reproduction in domestic animals 2017 v.52 Suppl S2 no. pp. 332-335
ISSN:
0936-6768
Subject:
blood serum; cats; contraception; females; gonadotropin-releasing hormone; luteinization; luteinizing hormone; secretion; spaying; temporal variation; vaccination; vaccines
Abstract:
... Measurement of circulating luteinizing hormone (LH) concentrations in cats and temporal changes following ovariohysterectomy (OHE) or possibly GnRH vaccination may be informative for assessing their fertility, contraception or sterilization status. In this study, serum LH concentrations were measured in domestic cats (n = 6) immediately prior to and up to 120 days post‐OHE. Basal LH concentrations ...
DOI:
10.1111/rda.12840

52. The nuclear and developmental competence of cumulus–oocyte complexes is enhanced by three‐dimensional coculture with conspecific denuded oocytes during in vitro maturation in the domestic cat model

Author:
MG Morselli; GC Luvoni; P Comizzoli
Source:
Reproduction in domestic animals 2017 v.52 Suppl S2 no. pp. 82-87
ISSN:
0936-6768
Subject:
alginates; barium; cats; coculture; embryogenesis; epididymis; in vitro fertilization; meiosis; metaphase; models; morula; oocytes; spermatozoa; telophase; viability; zygote
Abstract:
... The objective of the study was to assess the efficacy of coculture with conspecific cumulus‐denuded oocytes (CDOs) during in vitro maturation in a three‐dimensional system of barium alginate microcapsules on the in vitro embryo development of domestic cat cumulus–oocyte complexes (COCs). In Experiment I, COCs were cocultured with conspecific CDOs or cultured separately in a 3D system for 24 hr of ...
DOI:
10.1111/rda.12850

53. Urethral catheterization and sperm vitrification for simplified semen banking in felids

Author:
WF Swanson; HL Bateman; LM Vansandt
Source:
Reproduction in domestic animals 2017 v.52 Suppl S2 no. pp. 255-260
ISSN:
0936-6768
Subject:
Felidae; acrosome; agonists; catheters; cats; dexmedetomidine; freezing; in vitro fertilization; insemination; males; semen; sperm motility; straw; vitrification; xylazine
Abstract:
... Semen banking of domestic cats and wild felids represents a vital resource for their long‐term conservation, but current methods require access to advanced training and specialized equipment. A newer method of semen collection, urethral catheterization of medetomidine‐treated cats, allows recovery of high sperm numbers, but it is unclear if this approach permits maximal sperm recovery or is feasib ...
DOI:
10.1111/rda.12863

54. Viability and growth of feline preantral follicles in vitro cultured with insulin growth factor and epidermal growth factor supplemented medium

Author:
AE Alves; LC Padilha‐Nakaghi; EA Pires‐Butler; M Apparicio; NAM Silva; TF Motheo; WRR Vicente; GC Luvoni
Source:
Reproduction in domestic animals 2017 v.52 Suppl S2 no. pp. 93-97
ISSN:
0936-6768
Subject:
cats; culture media; epidermal growth factor; follicular development; granulosa cells; insulin; insulin-like growth factor I; oocytes; viability
Abstract:
... In vitro culture of ovarian preantral follicles has emerged as a reproductive technology aimed at obtaining large amount of oocytes for in vitro embryo production. The addition of growth factors (GF) in the in vitro culture of preantral follicles of different species has provided superior results of follicular development, antrum formation and proliferation of granulosa cells. However, there are o ...
DOI:
10.1111/rda.12839

55. A Rare Case of Polyorchidism in a Cat with Four Intra‐abdominal Testes

Author:
J Roca‐Ferrer; E Rodríguez; GA Ramírez; C Moragas; M Sala
Source:
Reproduction in domestic animals 2015 v.50 no.1 pp. 172-176
ISSN:
0936-6768
Subject:
case studies; cats; congenital abnormalities; testes; ultrasonography; veterinary medicine
Abstract:
... Polyorchidism is a rare congenital anomaly defined as the presence of more than two histologically proven testes. We report a case of a 9‐month‐old European cat with four intra‐abdominal testes. The diagnosis was performed by means of ultrasonography, intra‐operative examination and histological confirmation. The case reported here presents an extremely rare anomaly, as no previous studies in vete ...
DOI:
10.1111/rda.12461

56. Comparative Development of Embryonic Age by Organogenesis in Domestic Dogs and Cats

Author:
NCG Pieri; AF Souza; JB Casals; KCS Roballo; CE Ambrósio; DS Martins
Source:
Reproduction in domestic animals 2015 v.50 no.4 pp. 625-631
ISSN:
0936-6768
Subject:
anatomy and morphology; biotechnology; carnivores; cats; dogs; embryogenesis; gestational age; microscopy; organogenesis; pregnancy; prenatal development; veterinary clinics
Abstract:
... The precise determination of the embryonic chronology is very important in reproductive biotechnologies, especially in estimating embryonic age. Thus, there is a need for greater knowledge and standardization for determining the chronology of embryonic development and functional morphology. We describe aspects of embryonic development in two domestic carnivores to add knowledge about organ peculia ...
DOI:
10.1111/rda.12539

57. Comparison of DNA Fragmentation Assay in Frozen‐Thawed Cat Epididymal Sperm

Author:
P Kunkitti; A Sjödahl; A‐S Bergqvist; A Johannisson; E Axnér
Source:
Reproduction in domestic animals 2016 v.51 no.4 pp. 618-622
ISSN:
0936-6768
Subject:
DNA; DNA fragmentation; acridine orange; cats; chromatin; epididymis; spermatozoa; staining
Abstract:
... DNA fragmentation of frozen‐thawed feline epididymal sperm from corpus and cauda regions was evaluated by three different techniques. The DNA fragmentation index (DFI) was compared between techniques: the sperm chromatin structural assay (SCSA®), acridine orange staining techniques (AOT) and the sperm chromatin dispersion (SCD). There were significant differences in DFI among the techniques (p < 0 ...
DOI:
10.1111/rda.12710

58. Determination of gestational time and prediction of parturition in dogs and cats: an update

Author:
M. Beccaglia; S. Alonge; C. Trovo’; G. C. Luvoni
Source:
Reproduction in domestic animals 2016 v.51 Suppl S1 no. pp. 12-17
ISSN:
0936-6768
Subject:
bitches; breeding; cats; heart rate; medicine; neonates; ovulation; parturition; pets; prediction; pregnancy; radiography; radiology; small animal practice; surgery; ultrasonography; England
Abstract:
... Accurate prediction of delivery date in canine and feline allows a better management of parturition, reducing the loss of neonates. This review evaluates the most common methods adopted to accurately predict the day of delivery: determination of ovulation and hormonal assays, first appearance of embryonic/foetal structures using ultrasound or radiography, echographic measurement of extra‐foetal an ...
DOI:
10.1111/rda.12782

59. Endoscopic Transcervical Catheterization in the Domestic Cat

Author:
D Zambelli; C Bini; M Cunto
Source:
Reproduction in domestic animals 2015 v.50 no.1 pp. 13-16
ISSN:
0936-6768
Subject:
artificial insemination; catheters; cats; cervix; methodology; spermatozoa; veterinary clinics
Abstract:
... Feline‐assisted reproduction is still not routinely performed in veterinary practice, although there is an increasing interest on the subject by cat breeders. In recent years, many techniques for artificial insemination in the domestic cat have been developed with regard to the intrauterine deposition of sperm through the catheterization of the cervix. Transcervical catheterization has been descri ...
DOI:
10.1111/rda.12442

60. Influence of Different Protocols of Urethral Catheterization after Pharmacological Induction (Ur.Ca.P.I.) on Semen Quality in the Domestic Cat

Author:
M Cunto; DG Küster; C Bini; C Cartolano; M Pietra; D Zambelli
Source:
Reproduction in domestic animals 2015 v.50 no.6 pp. 999-1002
ISSN:
0936-6768
Subject:
catheters; cats; medetomidine; sedation; semen; spermatozoa; urethra
Abstract:
... The aim of this study was to evaluate the influence that different protocols of urethral catheterization after pharmacological induction (Ur.Ca.P.I.) may have on the semen quality of the domestic cat. The study has been divided into two experiments: one in which different dosages of medetomidine administrated are evaluated and the second one in which the timing of the catheterization after pharmac ...
DOI:
10.1111/rda.12626

61. Monitoring the Foetal Phase of Gestation in the Queen With a 12.5‐MHz Ultrasound Probe and Prediction of the Parturition by Combining the Measurements of Head and Abdominal Diameters

Author:
E Topie; D Bencharif; L Briand; D Tainturier
Source:
Reproduction in domestic animals 2015 v.50 no.4 pp. 560-566
ISSN:
0936-6768
Subject:
abdomen; calcification; cats; confidence interval; equations; head; monitoring; parturition; prediction; pregnancy; ultrasonics
Abstract:
... Ten gestations in six domestic shorthair cats (Europeans) were monitored daily during the foetal phase of gestation, from the 28th day after the first mating until parturition, using ultrasound with a 12.5‐MHz probe. The development of the various organs over this period was recorded. The diameters of the head (HD) and abdomen (AD) were measured. Skeletal calcification visible on ultrasound occurr ...
DOI:
10.1111/rda.12523

62. Orexin A Expression in the Ovary of Dog and Cat

Author:
M Levanti; A Germanà; F Abbate
Source:
Reproduction in domestic animals 2015 v.50 no.2 pp. 247-250
ISSN:
0936-6768
Subject:
amino acids; animal ovaries; cats; dogs; female genitalia; fluorescent antibody technique; hypothalamus; neuropeptides; receptors; tissues
Abstract:
... Orexin A and B, also known as hypocretin A and B, are hypothalamic neuropeptides arising from a precursor to the 130 amino acid, called pre–pro orexin. They are synthesized mainly in lateral and posterior hypothalamus and are involved in different functions such as regulation of food intake and energy balance. Orexins and orexin receptors were previously described also in different tissues and org ...
DOI:
10.1111/rda.12478

63. Successful Cryopreservation of Domestic Cat (Felis catus) Epididymal Sperm after Slow Equilibration to 15 or 10°C

Author:
C Klaus; S Eder; C Franz; K Müller
Source:
Reproduction in domestic animals 2016 v.51 no.2 pp. 195-203
ISSN:
0936-6768
Subject:
ambient temperature; cats; cooling; cryopreservation; egg yolk; freezing; glycerol; hens; low density lipoprotein; nitrogen; spermatozoa; thawing; water solubility
Abstract:
... To support conservation strategies in wild species, simple but highly reproducible procedures of sperm cryopreservation are required for an application under field conditions. We used epididymal sperm of the domestic cat to optimize a sperm freezing procedure for felid species, particularly questioning the demand for sperm cooling to 4°C. We equilibrated sperm during slow cooling to only 15 or 10° ...
DOI:
10.1111/rda.12666

64. Total Cell Number and its Allocation to Trophectoderm and Inner Cell Mass in In Vitro Obtained Cats' Blastocysts

Author:
M Ochota; B Wojtasik; W Niżański
Source:
Reproduction in domestic animals 2016 v.51 no.3 pp. 339-345
ISSN:
0936-6768
Subject:
blastomeres; cats; hatching; in vitro culture; trophoblast
Abstract:
... The aim of this study was to evaluate the developmental kinetics of cats' blastocysts in connection with their morphology and blastomeres allocation to trophoblast or embryoblast cells. We examined gross blastocyst morphology and the total number of blastomeres together with its allocation to inner cell mass (ICM) or trophectoderm (TE) cells in pre‐implantation feline embryos obtained from 6th to ...
DOI:
10.1111/rda.12684

65. Determination of Appropriate Cryopreservation Protocols for Epididymal Cat Spermatozoa

Author:
K Buranaamnuay
Source:
Reproduction in domestic animals 2015 v.50 no.3 pp. 378-385
ISSN:
0936-6768
Subject:
acrosome; cats; cryopreservation; eggs; freezing; glycerol; pellets; thawing; viability
Abstract:
... Effects of Equex and glycerol additions and sample dilution step on frozen–thawed epididymal cat spermatozoa were investigated. The epididymal sperm pellets were resuspended in extenders using one‐ (groups III and IV) or two‐ (groups I, II, V and VI) step dilution. For one‐step dilution, the pellets were resuspended in plain egg yolk‐Tris medium (EYT) + 5% glycerol with (IV)/without (III) 0.5% Equ ...
DOI:
10.1111/rda.12496

66. Doppler and Contrast‐Enhanced Ultrasonography of Testicles in Adult Domestic Felines

Author:
MBS Brito; MAR Feliciano; LN Coutinho; RR Uscategui; APR Simões; MC Maronezi; VT Almeida; RM Crivelaro; B Gasser; L Pavan; WR Russiano
Source:
Reproduction in domestic animals 2015 v.50 no.5 pp. 730-734
ISSN:
0936-6768
Subject:
adults; blood flow; cats; diagnostic techniques; spermatic cord; testes; ultrasonics; ultrasonography
Abstract:
... The objective was to characterize the vascular patterns of testicular blood flow of adult cats, measuring the systolic velocity (SV), diastolic velocity (DV), resistance index (RI), gate time (wash‐in) peak enhancement and output time (wash‐out) of the contrast and addition of tissue fill characteristics. Forty‐five adult cats were selected, and the echotexture, echogenicity, size, contours and ma ...
DOI:
10.1111/rda.12557

67. Endocrine Disruptors in Domestic Animal Reproduction: A Clinical Issue?

Author:
Ulf Magnusson; Sara Persson
Source:
Reproduction in domestic animals 2015 v.50 Suppl S3 no. pp. 15-19
ISSN:
0936-6768
Subject:
animal reproduction; cats; dogs; domestic animals; endocrine-disrupting chemicals; farmed animal species; pollutants; reproductive system
Abstract:
... The objective of this review was to discuss whether endocrine disruption is a clinical concern in domestic animal reproduction. To that end, we firstly summarize the phenomenon of endocrine disruption, giving examples of the agents of concern and their effects on the mammalian reproductive system. Then there is a brief overview of the literature on endocrine disruptors and domestic animal reproduc ...
DOI:
10.1111/rda.12563
PubMed:
26382024
PubMed Central:
PMC4584497

68. Immunohistochemical Expression of Cyclooxygenase‐2 (COX‐2) in Feline Endometrial Adenocarcinoma and in Normal and Hyperplastic Endometria

Author:
AL Saraiva; R Payan‐Carreira; F Gärtner; I Santana; A Rêma; LM Lourenço; MA Pires
Source:
Reproduction in domestic animals 2015 v.50 no.2 pp. 333-340
ISSN:
0936-6768
Subject:
adenocarcinoma; carcinogenesis; cats; cytoplasm; endometrium; epithelial cells; epithelium; humans; immunohistochemistry; myometrium; necrosis; neoplasm cells; prostaglandin synthase; sampling; serosa
Abstract:
... Cyclooxygenase‐2 (COX‐2) is overexpressed in several human and animal neoplasms, including the human endometrial carcinoma. It has been suggested as a prognostic marker and a potential therapeutic target. This study aimed to (i) clarify histological aspects of feline endometrial adenocarcinomas (FEA) of the papillary serous type and (ii) characterize COX‐2 immunohistochemical expression in normal, ...
DOI:
10.1111/rda.12497

69. Impact of Ultra‐Rapid Freezing on the Motility, Morphology, Viability and Acrosome Integrity of Epididymal Cat Sperm Diluted in Sucrose‐Based Extenders

Author:
G Vizuete; E Jiménez; EI Agüera; CC Pérez‐Marín
Source:
Reproduction in domestic animals 2014 v.49 no.1 pp. e5
ISSN:
0936-6768
Subject:
acrosome; cats; freezing; semen; sperm motility; sucrose; thawing; viability
Abstract:
... The objective of the present study was to investigate the influence of different sucrose‐based extenders on the motility, morphology, viability and acrosomal integrity of epididymal cat spermatozoa cryopreserved by ultra‐rapid freezing method. Nine cats were castrated, and collected semen was diluted 1 : 1 with Dulbecco`s phosphate‐buffered saline‐BSA1%‐based extender supplemented with different s ...
DOI:
10.1111/rda.12253
PubMed:
24256485

70. Presence of Membranous Vesicles in Cat Seminal Plasma: Ultrastructural Characteristics, Protein Profile and Enzymatic Activity

Author:
A Polisca; A Troisi; A Minelli; I Bellezza; A Fontbonne; R Zelli
Source:
Reproduction in domestic animals 2015 v.50 no.1 pp. 91-96
ISSN:
0936-6768
Subject:
acid phosphatase; aminopeptidases; cats; enzyme activity; male genitalia; particles; seminal plasma; transmission electron microscopy
Abstract:
... This study sought to verify the presence of membranous vesicles in cat seminal plasma by means of transmission electron microscopy and to identify protein profile and some of the enzymatic activities associated with these particles. The transmission electron microscopy observations showed the existence of different sized vesicular membranous structures of more or less spherical shape. These vesicl ...
DOI:
10.1111/rda.12453

71. Tiger, Bengal and Domestic Cat Embryos Produced by Homospecific and Interspecific Zona‐Free Nuclear Transfer

Author:
LN Moro; J Jarazo; C Buemo; MI Hiriart; A Sestelo; DF Salamone
Source:
Reproduction in domestic animals 2015 v.50 no.5 pp. 849-857
ISSN:
0936-6768
Subject:
Felis; Panthera tigris tigris; Prionailurus bengalensis; blastocyst; cats; hybrids; intracytoplasmic sperm injection; methodology
Abstract:
... The aim of this study was to evaluate three different cloning strategies in the domestic cat (Felis silvestris) and to use the most efficient to generate wild felid embryos by interspecific cloning (iSCNT) using Bengal (a hybrid formed by the cross of Felis silvestris and Prionailurus bengalensis) and tiger (Panthera tigris) donor cells. In experiment 1, zona‐free (ZP‐free) cloning resulted in hig ...
DOI:
10.1111/rda.12593

72. X monosomy in a Virilized Female Cat

Author:
I Szczerbal; W Nizanski; S Dzimira; J Nowacka‐Woszuk; M Ochota; M Switonski
Source:
Reproduction in domestic animals 2015 v.50 no.2 pp. 344-348
ISSN:
0936-6768
Subject:
X chromosome; cats; chromosome banding; chromosome painting; corpus luteum; females; fibroblasts; genes; genitalia; laparotomy; masculinization; monosomics; penis; testosterone; uterus; vagina; vulva
Abstract:
... An infertile Siamese female cat was subjected for clinical, histological, cytogenetic and molecular studies due to ambiguous external genitalia (vulva, vagina, rudimentary penis and scrotum‐like structure) and masculine behaviour. An elevated oestrogen activity and a detectable level of testosterone were found. The cat underwent laparotomy. The gonads and the uterus were removed and subjected for ...
DOI:
10.1111/rda.12483

73. Clinical Use of Deslorelin (GnRH agonist) in Companion Animals: A Review

Author:
X Lucas
Source:
Reproduction in domestic animals 2014 v.49 Suppl s4 no. pp. 64-71
ISSN:
0936-6768
Subject:
adverse effects; agonists; bitches; cats; contraception; estrus; gonadotropin-releasing hormone; pets
Abstract:
... Over the years, many contraceptive medications have been developed for companion animals, but many secondary adverse effects have limited their use. A major advancement was achieved with the use of gonadotropin‐releasing hormone (GnRH) analogues, mainly GnRH agonists, which mimic the effects of native GnRH. The development of effective low‐dose, slow‐release implants with potent agonists such as d ...
DOI:
10.1111/rda.12388
PubMed:
25277434

74. Determination of Feeder Cell‐Based Cellular Niches Supporting the Colonization and Maintenance of Spermatogonial Stem Cells from Prepubertal Domestic Cat Testes

Author:
NR Han; YH Park; JI Yun; HJ Park; MH Park; MS Kim; JH Choi; E Lee; SP Gong; JM Lim; ST Lee
Source:
Reproduction in domestic animals 2014 v.49 no.5 pp. 705-710
ISSN:
0936-6768
Subject:
alkaline phosphatase; cats; dogs; fibroblasts; genes; in vitro culture; mice; niches; stem cells; stromal cells; testes
Abstract:
... Recently, isolation and in vitro culture of putative spermatogonial stem cells (SSCs) in the domestic cat have been conducted. However, the cellular niche conditions that facilitate the establishment and long‐term maintenance of feline SSCs (FSSCs) have not been described. Therefore, we investigated the type of feeder cells used to stimulate colony formation and growth of FSSCs among the various f ...
DOI:
10.1111/rda.12351

75. Evaluation of Cheetah and Leopard Spermatozoa Developmental Capability after Interspecific ICSI with Domestic Cat Oocytes

Author:
LN Moro; AJ Sestelo; DF Salamone
Source:
Reproduction in domestic animals 2014 v.49 no.4 pp. 693-700
ISSN:
0936-6768
Subject:
Acinonyx jubatus; DNA fragmentation; Panthera pardus; blastocyst; cats; internal transcribed spacers; oocytes; oxygen; reproduction; spermatozoa
Abstract:
... The ICSI procedure is potentially of great value for felids, and it has not been extensively studied in these species. The objectives of this work were to determine the best conditions for ICSI in the domestic cat (DC) to generate interspecific embryos by injecting cheetah (Ch) and leopard (Leo) spermatozoa. Firstly, DC oocytes were matured with insulin–transferrin–selenium (ITS) or without it (MM ...
DOI:
10.1111/rda.12355
PubMed:
24966115

76. Expression of Survivin, Bcl‐2 and Bax Proteins in the Domestic Cat (Felis catus) Endometrium During the Oestrus Cycle

Author:
N Liman; E Alan; GK Bayram; K Gürbulak
Source:
Reproduction in domestic animals 2013 v.48 no.1 pp. 33-45
ISSN:
0936-6768
Subject:
DNA fragmentation; apoptosis; blood vessels; cats; cell proliferation; endometrium; epithelial cells; estrus; myocytes; proteins; smooth muscle
Abstract:
... Apoptosis has been shown to be an important regulator of endometrium function. To clarify the regulation of apoptosis in the cat endometrium during the normal oestrus cycle, the expressions of the apoptosis‐related proteins (Bcl‐2 and Bax) and their correlation to the inhibitor of apoptosis protein Survivin were analysed using immunohistochemistry. The TUNEL technique (TdT‐mediated dUTP nick end l ...
DOI:
10.1111/j.1439-0531.2012.02021.x
PubMed:
22471444

77. Immunolocalization of Aquaporin Water Channels in the Domestic Cat Male Genital Tract

Author:
S Arrighi; M Aralla
Source:
Reproduction in domestic animals 2014 v.49 no.1 pp. 17-26
ISSN:
0936-6768
Subject:
Primates; antibodies; aquaporins; blood vessels; caput epididymidis; cats; cauda epididymidis; dogs; epithelium; male genitalia; rete testis; smooth muscle
Abstract:
... Four different aquaporins (AQP1, 2, 5 and 9), integral membrane water channels that facilitate rapid passive movement of water, were immuno‐localized in the excurrent ducts collected from sexually mature cats during orchiectomy. Aquaporins 1, 2 and 9, were immuno‐localized at distinct levels, whereas AQP5 was undetectable all along the feline genital tract. No immunoreactivity was present at the l ...
DOI:
10.1111/rda.12213
PubMed:
23826797

78. Intermediate Filament Proteins Expression and Carbohydrate Moieties in Trophoblast and Decidual Cells of Mature Cat Placenta

Author:
PE Fernández; ME Diessler; A Pachame; HH Ortega; EJ Gimeno; EL Portiansky; CG Barbeito
Source:
Reproduction in domestic animals 2014 v.49 no.2 pp. 263-269
ISSN:
0936-6768
Subject:
Phaseolus vulgaris; acids; antibodies; cats; cytoskeleton; fibroblasts; galactosamine; galactose; giant cells; glucosamine; glycoconjugates; glycosides; glycosylation; humans; hysterectomy; immunohistochemistry; lectins; mice; oligosaccharides; polysaccharides; pregnancy; rats; trophoblast; uterus; vimentin
Abstract:
... The aim of this study was to characterize cytoskeletal intermediate filament proteins and glycoconjugates of syncytiotrophoblast, cytotrophoblast and decidual cells of feline endotheliochorial placenta. Samples from 12 normal pregnant female cats, after 45 ± 5 days of gestation, were obtained removing the uterine horns by hysterectomy. Sections were processed for routine observation and for immuno ...
DOI:
10.1111/rda.12265
PubMed:
24471554

79. Stem Cells and Regenerative Medicine in Domestic and Companion Animals: A Multispecies Perspective

Author:
NN Gonçalves; CE Ambrósio; JA Piedrahita
Source:
Reproduction in domestic animals 2014 v.49 Suppl s4 no. pp. 2-10
ISSN:
0936-6768
Subject:
cats; dogs; embryonic stem cells; horses; humans; induced pluripotent stem cells; medicine; mice; models; pets; swine; veterinary medicine
Abstract:
... Since their original isolation, the majority of the work on embryonic stem cells (ESC) has been carried out in mice. While the mouse is an outstanding model for basic research, it also has considerable limitations for translational work, especially in the area of regenerative medicine. This is due to a combination of factors that include physiological and size differences when compared to humans. ...
DOI:
10.1111/rda.12392
PubMed:
25277427

80. Suppression of Fertility in Adult Cats

Author:
S Goericke‐Pesch; A Wehrend; P Georgiev
Source:
Reproduction in domestic animals 2014 v.49 Suppl s2 no. pp. 33-40
ISSN:
0936-6768
Subject:
adults; agonists; antagonists; breeding; cats; estrus; gonadotropin-releasing hormone; immunocontraception; melatonin; progestational hormones
Abstract:
... Cats are animals with highly efficient reproduction, clearly pointing to a need for suppression of fertility. Although surgical contraception is highly effective, it is not always the method of choice. This is predominantly because it is cost‐intensive, time‐consuming and irreversible, with the latter being of major importance for cat breeders. This article reviews the use of progestins, sclerotin ...
DOI:
10.1111/rda.12301
PubMed:
24947859

81. Suppression of Fertility in Pre‐pubertal Dogs and Cats

Author:
S Schäfer‐Somi; D Kaya; N Gültiken; S Aslan
Source:
Reproduction in domestic animals 2014 v.49 Suppl s2 no. pp. 21-27
ISSN:
0936-6768
Subject:
agonists; bitches; breeding stock; cats; epiphyses; estrus; genetic background; gonadotropin-releasing hormone; pets; puberty; resorption
Abstract:
... Pre‐pubertal gonadectomy in dogs and cats is still controversially discussed because some consequences cause health problems. Nevertheless, postponement of puberty, that is, prevention of an increase in sexual hormones and thereby prevention of their manifold effects, is of major importance, not only in controlling overpopulation but also to preserve the genetic base for future breeding stock and ...
DOI:
10.1111/rda.12330
PubMed:
24947857

82. Transcervical Artificial Insemination in Dogs and Cats: Review of the Technique and Practical Aspects

Author:
S Romagnoli; C Lopate
Source:
Reproduction in domestic animals 2014 v.49 Suppl s4 no. pp. 56-63
ISSN:
0936-6768
Subject:
artificial insemination; bitches; catheters; cats; cervix; endoscopy; estrus; humans; image analysis; rectum; vaginal mucosa
Abstract:
... The technique of transcervical catheterization for artificial insemination has gained practical importance over the last 3 and 1 decades in bitches and queens, respectively. The vagina of both species has a thickening of the dorsal aspect called dorsal medial fold, which restricts the lumen of the paracervix, making catheterization of the cervix difficult both with manual and endoscopic techniques ...
DOI:
10.1111/rda.12395
PubMed:
25277433

83. Gonadotropin‐Releasing Hormone Targeting for Gonadotroph Ablation: An Approach to Non‐surgical Sterilization

Author:
RS Struthers
Source:
Reproduction in domestic animals 2012 v.47 Suppl s4 no. pp. 233-238
ISSN:
0936-6768
Subject:
cats; conjugated proteins; cytotoxicity; developing countries; dogs; gonadotropic cells; gonadotropin-releasing hormone; neoplasms; pituitary gonadotropins; rats; receptors; testosterone; therapeutics
Abstract:
... Surgical sterilization is the mainstay of dog and cat population control, but its use is still often limited by the costs and effort involved, especially in developing countries. An ideal non‐surgical sterilant that is safe, effective, permanent, administered as a single injection and capable of being manufactured inexpensively could have a significant impact on the world‐wide dog and cat overpopu ...
DOI:
10.1111/j.1439-0531.2012.02081.x
PubMed:
22827376

84. Long‐Term‐Release GnRH Agonists Postpone Puberty in Domestic Cats

Author:
A Risso; Y Corrada; C Barbeito; JD Diaz; C Gobello
Source:
Reproduction in domestic animals 2012 v.47 no.6 pp. 936-938
ISSN:
0936-6768
Subject:
acetates; adults; agonists; animal ovaries; body weight; cats; cell biology; gonadotropin-releasing hormone; ovariectomy; photoperiod; puberty; pyometra
Abstract:
... The aim of this study was to assess the efficacy and safety of deslorelin acetate implants on domestic queen puberty postponement. Thirty, 114.4 ± 12.7 days old, 1.5 ± 0.1 kg prepubertal crossbred female cats were included in this study. The animals were kept under a positive photoperiod and randomly assigned to deslorelin acetate 4.7 mg SC implants (n = 15) or to a non‐treated control group (n =  ...
DOI:
10.1111/j.1439-0531.2012.01994.x
PubMed:
22335549

85. Prostaglandin Endoperoxide Synthase 2 (PTGS2) and Prostaglandins F2α and E2 Synthases (PGFS and PGES) Expression and Prostaglandin F2α and E2 Secretion Following Oestrogen and/or Progesterone Stimulation of the Feline Endometrium

Author:
MJ Siemieniuch; E Jursza; MP Kowalewski; M Majewska; DJ Skarzynski
Source:
Reproduction in domestic animals 2013 v.48 no.1 pp. 72-78
ISSN:
0936-6768
Subject:
animal ovaries; arachidonic acid; cats; endometrium; gene expression; genes; in vitro culture; messenger RNA; metabolism; progesterone; prostaglandins; pseudopregnancy; secretion; steroids
Abstract:
... Sex steroids in synergy with prostaglandins (PG) are involved in the regulation of cyclic ovarian function. In this study, we investigated the mRNA expression of three genes involved in arachidonic acid (AA) metabolism and hence PG production in domestic cats: PG‐endoperoxide synthase (PTGS2), PGF₂α synthase (PGFS) and PGE₂ synthase (PGES). Feline endometria (n = 16) were collected at oestrus and ...
DOI:
10.1111/j.1439-0531.2012.02031.x
PubMed:
22583354

86. Clinical Use of GnRH Agonists in Canine and Feline Species

Author:
Fontaine, E.; Fontbonne, A.
Source:
Reproduction in domestic animals 2011 v.46 no.2 pp. 344-353
ISSN:
0936-6768
Subject:
agonists; animal reproduction; blood flow; cats; dogs; follicle-stimulating hormone; gonadotropin-releasing hormone; luteinizing hormone; secretion; steroid hormones
Abstract:
... GnRH (gonadotrophin releasing hormone) is a key hormone of reproductive function in mammals; agonist forms have been largely developed, and data concerning their use in small animal reproduction are now abundant. GnRH agonists act by a two-step mechanism. First, their agonist properties on the pituitary will cause marked LH (luteinizing hormone) and FSH (follicle-stimulating hormone) secretion int ...
DOI:
10.1111/j.1439-0531.2010.01705.x
PubMed:
20964727

87. Cryopreservation of Cat Testicular Tissues: Effects of Storage Temperature, Freezing Protocols and Cryoprotective Agents

Author:
P Thuwanut; K Chatdarong
Source:
Reproduction in domestic animals 2012 v.47 no.5 pp. 777-781
ISSN:
0936-6768
Subject:
DNA; Felidae; acridine orange; cats; cryopreservation; cutting; dimethyl sulfoxide; ethylene glycol; freezing; nitrogen; plasma membrane; spermatozoa; storage temperature; testes; transportation; vapors; vitrification; wild animals
Abstract:
... Cryopreservation of testicular tissue has become a part of gamete preservation in wild animal post‐mortem. Using domestic cats as a model for wild felids, this study aimed to (i) investigate the effect of temperature for testicular tissue storage on sperm quality; (ii) compare efficiency of freezing protocols; and (iii) evaluate properties of cryoprotective agents to protect testicular sperm quali ...
DOI:
10.1111/j.1439-0531.2011.01967.x
PubMed:
22151534

88. Effect of Vitrification of Feline Ovarian Cortex on Follicular and Oocyte Quality and Competence

Author:
GC Luvoni; I Tessaro; M Apparício; E Ruggeri; AM Luciano; SC Modina
Source:
Reproduction in domestic animals 2012 v.47 no.3 pp. 385-391
ISSN:
0936-6768
Subject:
actin; biodiversity; cats; chromatin; cortex; cryopreservation; follicular development; freezing; germinal vesicle; meiosis; microfilaments; microtubules; ovarian follicles; vitrification
Abstract:
... Cryopreservation of ovarian cortex has important implications in the preservation of fertility and biodiversity in animal species. Slow freezing of cat ovarian tissue resulted in the preservation of follicular morphology and in the follicular development after xenografting. Vitrification has been recently applied to ovarian tissues of different species, but no information is available on the effec ...
DOI:
10.1111/j.1439-0531.2011.01885.x
PubMed:
21950518

89. Effects of Surgical Sterilization on Canine and Feline Health and on Society

Author:
MV Root Kustritz
Source:
Reproduction in domestic animals 2012 v.47 Suppl s4 no. pp. 214-222
ISSN:
0936-6768
Subject:
animal health; castration; cats; dogs; society; spaying
Abstract:
... Surgical sterilization of dogs and cats is a well‐accepted measure for population control in some countries, but is considered unethical as an elective surgery in other countries. This is a review of what is known regarding positive and negative effects of gonadectomy surgery on individual animals and on societal management of unowned dog and cat populations. ...
DOI:
10.1111/j.1439-0531.2012.02078.x
PubMed:
22827373

90. Exuberant Mucometra Associated with Atresia of the Cervix in a Queen

Author:
M Batista‐Arteaga; M Santana; A Espinosa‐de‐los‐Monteros; S Déniz; D Alamo; P Herráez
Source:
Reproduction in domestic animals 2012 v.47 no.5 pp. e71
ISSN:
0936-6768
Subject:
abnormal development; animal ovaries; blood; cats; cervix; histopathology; hyperplasia; ovarian cysts; spaying; ultrasonics; uterus
Abstract:
... This short communication reports the clinical, ultrasonographic and histopathological findings in a cat with atresia of the uterine cervix and mucometra. After 6 months of continuous oestrous behaviour, a remarkable abdominal enlargement was observed in a 14‐year‐old queen. A presumptive diagnosis of mucometra was concluded after the ultrasound evaluation and based on clinical signs and blood anal ...
DOI:
10.1111/j.1439-0531.2011.01942.x
PubMed:
22107033

91. Isolation and Culture of Preantral Follicles for Retrieving Oocytes for the Embryo Production: Present Status in Domestic Animals

Author:
PSP Gupta; S Nandi
Source:
Reproduction in domestic animals 2012 v.47 no.3 pp. 513-519
ISSN:
0936-6768
Subject:
buffaloes; camels; cats; cattle; dogs; goats; horses; oocytes; sheep; swine
Abstract:
... The development of efficient ovarian preantral follicle (PF) isolation and culture systems provide a large number of oocytes for the manipulation and embryo production. It also helps for understanding the mechanisms of follicle and oocyte development. Isolation and culture protocols for PFs were developed for many domestic species like cattle, buffalo, sheep, goat, pig, horse, camel, dog and cats; ...
DOI:
10.1111/j.1439-0531.2011.01904.x
PubMed:
22008238

92. Progress in Development of Immunocontraceptive Vaccines for Permanent Non‐surgical Sterilization of Cats and Dogs

Author:
MW Munks
Source:
Reproduction in domestic animals 2012 v.47 Suppl s4 no. pp. 223-227
ISSN:
0936-6768
Subject:
cats; contraceptive vaccines; developed countries; dogs; immune system
Abstract:
... Each year, millions of cats and dogs are euthanized worldwide. There are insufficient resources to control shelter animals in developed countries, as well as feral cat and wild dog population levels, with current surgical sterilization techniques. Thus, population control of these animals will likely depend on the development of new non‐surgical methods for cat and dog sterilization. One promising ...
DOI:
10.1111/j.1439-0531.2012.02079.x
PubMed:
22827374

93. Reproductive Technologies Relevant to the Genome Resource Bank in Carnivora

Author:
S Amstislavsky; H Lindeberg; GC Luvoni
Source:
Reproduction in domestic animals 2012 v.47 no.1 pp. 164-175
ISSN:
0936-6768
Subject:
Canidae; Mustelidae; Panthera tigris; Ursidae; assisted reproductive technologies; cats; dogs; economic valuation; ecosystems; endangered species; farmed animal species; ferrets; foxes; fur; genome; humans; industry; laboratory animals; mink; pets; predators; reproductive traits; rodents
Abstract:
... Carnivora is one of the most species‐rich order of Mammalia. Some species, e.g. domestic cats, dogs and ferrets, are among the most popular pets; others, such as minks and farmed foxes, have economic value for the fur breeding industry. Still others, such as tigers, bears and other top predators, have great impact on the health of natural ecosystems. Most if not all Carnivora species have great cu ...
DOI:
10.1111/j.1439-0531.2011.01886.x
PubMed:
21883515

94. Targeted Gene Silencing to Induce Permanent Sterility

Author:
GA Dissen; A Lomniczi; RL Boudreau; YH Chen; BL Davidson; SR Ojeda
Source:
Reproduction in domestic animals 2012 v.47 Suppl s4 no. pp. 228-232
ISSN:
0936-6768
Subject:
brain; cats; dogs; gene silencing; gene therapy; genes; periodicity; reproduction; sterilizing
Abstract:
... A non‐surgical method to induce sterility would be a useful tool to control feral populations of animals. Our laboratories have experience with approaches aimed at targeting brain cells in vivo with vehicles that deliver a payload of either inhibitory RNAs or genes intended to correct cellular dysfunction. A combination/modification of these methods may provide a useful framework for the design of ...
DOI:
10.1111/j.1439-0531.2012.02080.x
PubMed:
22827375
PubMed Central:
PMC3516287

95. Tetrathyridiosis and Tubulopapillary Carcinoma Occurring Simultaneously in the Mammary Gland of a Cat

Author:
H Avci; N Toplu
Source:
Reproduction in domestic animals 2012 v.47 no.3 pp. e36
ISSN:
0936-6768
Subject:
Mesocestoides; carcinoma; cats
Abstract:
... This report describes a case of tubulopapillary carcinoma and concomitant tetrathyridiosis in a 5‐year‐old female cross‐breed cat. A mass was located at right inguinal mammary gland and measured 5.5 × 5 × 3 cm in size with multilobulated to solid appearance. The cut surface of the mass had a centrally located large cyst (approximately 3 cm in diameter) surrounded by other smaller cysts. Histologic ...
DOI:
10.1111/j.1439-0531.2011.01903.x
PubMed:
22497625

96. Vitrification with DAP 213 and Cryotop of Ex Situ and In Situ Feline Cumulus–Oocyte Complexes

Author:
AE Alves; AC Kozel; GC Luvoni
Source:
Reproduction in domestic animals 2012 v.47 no.6 pp. 1003-1008
ISSN:
0936-6768
Subject:
cortex; cats; dimethyl sulfoxide; propylene glycol; vitrification; meiosis; oocytes
Abstract:
... This study was undertaken to compare cryotolerance, in terms of viability and resumption of meiosis after warming and culture (24 and 48 h), of ex situ (isolated) and in situ (enclosed in the ovarian tissue) feline cumulus–oocyte complexes (COCs) vitrified with DAP 213 (2 m DMSO, 1 m acetamide, 3 m propylene glycol) in cryotubes or Cryotop method. Ovaries were harvested from 49 pubertal queens. Of ...
DOI:
10.1111/j.1439-0531.2012.02006.x
PubMed:
22384830

97. Alterations of Spindle and Microfilament Assembly in Aged Cat Oocytes

Author:
Jin, Y-X.; Cui, X-S.; Yu, X-F.; Han, Y-J.; Kong, I-K.; Kim, N-H.
Source:
Reproduction in domestic animals 2010 v.45 no.5 pp. 865-871
ISSN:
0936-6768
Subject:
Western blotting; cats; chromatin; cortex; germinal vesicle; metaphase; microfilaments; microtubules; mitogen-activated protein kinase; parthenogenesis; pronucleus
Abstract:
... To obtain insights into the cytoplasmic maturation status of cat oocytes recovered from cat ovaries following hormone treatment, we first examined microtubule and microfilament assembly in cat oocytes recovered from hormone-treated ovaries at various stages of maturation. Additionally, we determined the alteration of spindle and microfilament assembly, as well as mitogen-acitvated protein kinase ( ...
DOI:
10.1111/j.1439-0531.2009.01400.x
PubMed:
21457360

98. Development of New Stem Cell-based Technologies for Carnivore Reproduction Research

Author:
Travis, AJ; Kim, Y.; Meyers-Wallen, V.
Source:
Reproduction in domestic animals 2009 v.44 no.s2 pp. 22-28
ISSN:
0936-6768
Subject:
endangered species; cats; dogs; cell transplantation; ferrets; human health; assisted reproductive technologies; transgenic animals; xenotransplantation; animal models; embryonic stem cells; spermatozoa; biomedical research; carnivores; testes
Abstract:
... New reproductive technologies based on stem cells offer several potential benefits to carnivore species. For example, development of lines of embryonic stem cells in cats and dogs would allow for the generation of transgenic animal models, which could be used to advance both veterinary and human health. Techniques such as spermatogonial stem cell transplantation (SSCT) and testis xenografting offe ...
DOI:
10.1111/j.1439-0531.2009.01396.x
PubMed:
19754531
PubMed Central:
PMC2762641

99. Effect of Catalase and Superoxide Dismutase on Motility, Viability and Acrosomal Integrity of Frozen-Thawed Cat Spermatozoa

Author:
Thiangtum, K.; Pinyopummin, A.; Hori, T.; Kawakami, E.; Tsutsui, T.
Source:
Reproduction in domestic animals 2009 v.44 no.s2 pp. 369-372
ISSN:
0936-6768
Subject:
antioxidant activity; antioxidants; artificial vagina; catalase; cats; citrates; cryopreservation; egg yolk; freezing; glycerol; semen; sperm motility; spermatozoa; superoxide dismutase; thawing; viability
Abstract:
... The aim of this study was to evaluate the effect of antioxidant catalase (CAT) and superoxide dismutase (SOD) in semen extender on motility, viability and acrosomal integrity of frozen-thawed cat spermatozoa. Semen was collected by using an artificial vagina from five domestic cats (two ejaculates/cat). Spermatozoa were diluted in egg yolk Ttris-fructose citrate solution (EYT-FC) without glycerol ...
DOI:
10.1111/j.1439-0531.2009.01420.x
PubMed:
19754607

100. Effect of Progesterone and Ionomycin on Domestic Cat Sperm Motility Patterns and Acrosome Reaction

Author:
Villaverde, AISB; Fioratti, EG; Landim-Alvarenga, FC; de Carvalho Balieiro, JC; Melo, CM; Papa, FO; Lopes, MD
Source:
Reproduction in domestic animals 2009 v.44 no.s2 pp. 309-312
ISSN:
0936-6768
Subject:
acrosome reaction; artificial vagina; carbon dioxide; cats; ethanol; membrane potential; progesterone; sperm motility; spermatozoa
Abstract:
... This study was aimed at assessing the changes in sperm motion patterns and the percentage of acrosome reaction (AR) in domestic cat semen after treatment with either ionomycin or progesterone (P₄). Ten ejaculates were collected from five tomcats using an artificial vagina, and were diluted, centrifuged and resuspended in a capacitation medium. Samples were evaluated and divided into seven equal al ...
DOI:
10.1111/j.1439-0531.2009.01456.x
PubMed:
19754592