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You searched for: Journal Research in veterinary science Remove constraint Journal: Research in veterinary science Publication Year 2016 Remove constraint Publication Year: 2016 Source 2016 v.106 Remove constraint Source: 2016 v.106
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1. Acaricidal activity of leaf extracts of Dalbergia sissoo Roxb. (Fabaceae) against synthetic pyrethroid resistant Rhipicephalus (Boophilus) microplus

Author:
Singh, Nirbhay Kumar; Jyoti,; Vemu, Bhaskar; Prerna, Mranalini; Singh, Harkirat; Dumka, V.K.; Sharma, S.K.
Source:
Research in veterinary science 2016 v.106 pp. 1-6
ISSN:
0034-5288
Subject:
Dalbergia sissoo; Rhipicephalus microplus; acaricidal properties; adults; cypermethrin; deltamethrin; egg weight; eggs; ethanol; fecundity; hatching; larvae; leaf extracts; leaves; lethal concentration 50; mortality; oviposition; pyrethrins; ticks
Abstract:
... Resistance status of Rhipicephalus (Boophilus) microplus against synthetic pyrethroids was assessed by larval packet test which revealed level I and II resistance against cypermethrin and deltamethrin, respectively. Adult immersion test was employed to study the acaricidal activity of leaf extracts of Dalbergia sissoo (sheesham) against these ticks. Mortality and fecundity of ticks exposed to shee ...
DOI:
10.1016/j.rvsc.2016.03.002

2. Agreement of manual cell counts and automated counts of the scil Vet abc Plus+ hematology analyzer for analysis of equine synovial fluid

Author:
Van de Water, Eline; Oosterlinck, Maarten; Duchateau, Luc; Pille, Frederik
Source:
Research in veterinary science 2016 v.106 pp. 62-65
ISSN:
0034-5288
Subject:
synovial fluid; inflammation; blood cells; hematology; hyaluronoglucosaminidase; animal care; horses; France
Abstract:
... The purpose of this study was to determine whether the scil Vet abc Plus+ (SCIL Animal Care Company, Altorf, France), an impedance hematology analyzer, can accurately quantify and differentiate nucleated blood cells (NBCs) in equine synovial fluid. Synovial fluid samples (n=242) in different stages of experimentally induced inflammation were analyzed with and without hyaluronidase pretreatment and ...
DOI:
10.1016/j.rvsc.2016.03.012

3. Alterations in PTEN, MDM2, TP53 and AR protein and gene expression are associated with canine prostate carcinogenesis

Author:
Rivera-Calderón, Luis Gabriel; Fonseca-Alves, Carlos Eduardo; Kobayashi, Priscila Emiko; Carvalho, Marcio; Drigo, Sandra Aparecida; de Oliveira Vasconcelos, Rosemeri; Laufer-Amorim, Renée
Source:
Research in veterinary science 2016 v.106 pp. 56-61
ISSN:
0034-5288
Subject:
Western blotting; atrophy; carcinogenesis; dogs; gene expression; genes; humans; immunohistochemistry; men; models; prostatic neoplasms; protein synthesis; proteins; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; staining; therapeutics
Abstract:
... The PTEN, AR, MDM2 and p53 protein network plays a central role in the development of many human cancers, thus eliciting the development of targeted cancer therapeutics. Dogs spontaneously develop tumours, and they are considered a good model for comparative oncology initiatives. Due to the limited information on these proteins in canine tumours, this study aimed to investigate gene and protein al ...
DOI:
10.1016/j.rvsc.2016.03.008

4. An outbreak of bovine tuberculosis in a fallow deer herd (Dama dama) in Sicily

Author:
Amato, Benedetta; Mignacca, Sebastian Alessandro; Pacciarini, Maria Lodovica; Vitale, Maria; Antoci, Salvatore; Cucinotta, Salvatore; Puleio, Roberto; Biasibetti, Elena; Fiasconaro, Michele; Capucchio, Maria Teresa; Di Marco Lo Presti, Vincenzo
Source:
Research in veterinary science 2016 v.106 pp. 116-120
ISSN:
0034-5288
Subject:
Cervus dama; Mycobacterium bovis; bovine tuberculosis; cachexia; dead animals; epidemiology; genotyping; granuloma; herds; humans; monitoring; polymerase chain reaction; risk; ruminants; skin lesions; skin tests; tissues; wildlife; zoonoses; Sicily
Abstract:
... Wild ruminants have an important role in the epidemiology of bovine tuberculosis (bTB). This study describes an outbreak of bovine tuberculosis occurring in a fallow deer herd in Sicily. In 2012 a Sicilian herd of 47 animals was referred for cachexia. Pathological examination of 2 dead animals revealed disseminated granulomas predominantly involving the skin and subcutaneous tissues. Tissue sample ...
DOI:
10.1016/j.rvsc.2016.03.019

5. Antibodies reacting to carbonic anhydrase isozymes (I and II) and albumin in sera from dogs

Author:
Nishita, Toshiho; Miyazaki, Rui; Miyazaki, Takae; Ochiai, Hideharu; Orito, Kensuke
Source:
Research in veterinary science 2016 v.106 pp. 180-182
ISSN:
0034-5288
Subject:
Beagle; albumins; antibodies; atopic dermatitis; blood serum; carbonate dehydratase; diabetes; diarrhea; dogs; enzyme-linked immunosorbent assay; immunoglobulin G; isozymes; kidney diseases; pancreatitis; thyroid gland; vomiting
Abstract:
... IgGs to carbonic anhydrase isozymes (CA-I and CA-II) and albumin were identified in dog serum. IgG titers were determined in the sera of asymptomatic dogs, and in dogs with atopic dermatitis, diarrhea and/or vomiting, diabetes and/or pancreatitis, kidney disease, hepatic disease, and thyroid gland disease, using ELISA. Low titres of IgG-reactive CA-I, CA-II, BSA, and CSA were found in the sera of ...
DOI:
10.1016/j.rvsc.2016.04.010

6. Attitudes of Danish pig farmers towards requirements for hospital pens

Author:
Thomsen, Peter T.; Klottrup, Anne; Steinmetz, Henriette; Herskin, Mette S.
Source:
Research in veterinary science 2016 v.106 pp. 45-47
ISSN:
0034-5288
Subject:
cooling; farmers; farmers' attitudes; hospitals; labor; laws and regulations; questionnaires; swine
Abstract:
... According to Danish legislation, sick or injured pigs must be housed in hospital pens with specific requirements. During recent years the majority of cases of non-compliance with legislation have been related to management of these animals. Hence, we hypothesized that 1) pig farmers generally find a requirement for hospital pens reasonable, but do not know the specific requirements; 2) pig farmers ...
DOI:
10.1016/j.rvsc.2016.03.005

7. Cholinesterase in porcine saliva: Analytical characterization and behavior after experimental stress

Author:
Tecles, Fernando; Escribano, Damián; Martínez-Miró, Silvia; Hernández, Fuensanta; Contreras, María Dolores; Cerón, José Joaquín
Source:
Research in veterinary science 2016 v.106 pp. 23-28
ISSN:
0034-5288
Subject:
cholinesterase; detection limit; iodides; isozymes; models; nose; physostigmine; protein content; saliva; swine
Abstract:
... The purpose of this study was to measure and characterize the enzyme cholinesterase (ChE) in porcine saliva, as well as to evaluate its behavior in experimental stressful conditions. The results of ChE characterization by using different substrates and the selective inhibitors ethopropazine and physostigmine showed that the main enzyme existing in porcine saliva was butyrylcholinesterase (BChE). A ...
DOI:
10.1016/j.rvsc.2016.03.006

8. Circulating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluation

Author:
Buač, Marijana; Mojsilović, Slavko; Mišić, Dušan; Vuković, Dejan; Savić, Olivera; Valčić, Olivera; Marković, Dragana; Gvozdić, Dragan; Ilić, Vesna; Fratrić, Natalija
Source:
Research in veterinary science 2016 v.106 pp. 135-142
ISSN:
0034-5288
Subject:
adhesion; antigen-antibody complex; apoptosis; bronchopneumonia; calves; cows; granulocytes; in vitro studies; mononuclear leukocytes; necrosis; polystyrenes; propidium; staining; viability
Abstract:
... In this work we studied if circulating immune complexes (CIC) of calves with bronchopneumonia have the capacity to modulate function of peripheral blood leukocytes of healthy cattle.CIC of three month old calves (6 healthy and 6 diseased) were isolated by PEG precipitation. Peripheral blood mononuclear cells (MNCs) and granulocytes from healthy calves and cows were the CIC responder cells in in vi ...
DOI:
10.1016/j.rvsc.2016.04.002

9. Cloning and characterization of giant panda (Ailuropoda melanoleuca) IL-18 binding protein

Author:
Yan, Yue; Deng, Jiabo; Niu, Lili; Wang, Qiang; Yu, Jianqiu; Shao, Huanhuan; Cao, Qinghua; Zhang, Yizheng; Tan, Xuemei
Source:
Research in veterinary science 2016 v.106 pp. 170-172
ISSN:
0034-5288
Subject:
Ailuropoda melanoleuca; Escherichia coli; adaptive immunity; amino acid sequences; binding proteins; chromatography; endangered species; etiology; immune response; interferon-gamma; interleukin-18; mice; nickel; open reading frames; pathogenesis; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; spleen; splenocytes
Abstract:
... The giant panda (Ailuropoda melanoleuca) is an endangered species. Interleukin-18 (IL-18) plays an important role in the innate and adaptive immune responses by inducing IFN-γ. IL-18 has been implicated in the pathogenesis of various diseases. IL-18 binding protein (IL-18BP) is an intrinsic inhibitor of IL-18 that possesses higher affinity to IL-18. In this study, we cloned and characterized IL-18 ...
DOI:
10.1016/j.rvsc.2016.04.004
PubMed:
27234556
PubMed Central:
PMC7111782

10. Construction and characterization of a full-length infectious cDNA clone of foot-and-mouth disease virus strain O/JPN/2010 isolated in Japan in 2010

Author:
Nishi, Tatsuya; Onozato, Hiroyuki; Ohashi, Seiichi; Fukai, Katsuhiko; Yamada, Manabu; Morioka, Kazuki; Kanno, Toru
Source:
Research in veterinary science 2016 v.106 pp. 165-169
ISSN:
0034-5288
Subject:
Foot-and-mouth disease virus; RNA; antibodies; complementary DNA; fluorescent antibody technique; genetic engineering; genome; neutralization; pathogenicity; swine; transfection; vaccines; virus replication; viruses; Japan
Abstract:
... A full-length infectious cDNA clone of the genome of a foot-and-mouth disease virus isolated from the 2010 epidemic in Japan was constructed and designated pSVL-f02. Transfection of Cos-7 or IBRS-2 cells with this clone allowed the recovery of infectious virus. The recovered virus had the same in vitro characterization as the parental virus with regard to antigenicity in neutralization and indirec ...
DOI:
10.1016/j.rvsc.2016.03.017

11. Determination of minimum alveolar concentration of isoflurane in dogs and cats using the up-and-down method. A preliminary study

Author:
Barletta, Michele; Quandt, Jane; Hofmeister, Erik
Source:
Research in veterinary science 2016 v.106 pp. 81-83
ISSN:
0034-5288
Subject:
anesthesia; cats; dogs; isoflurane; oxygen; people; tail
Abstract:
... Minimum alveolar concentration (MAC) is a reliable measurement of the potency of inhaled anesthetic agents. The determination of MAC in different species has followed a fairly consistent methodology. In people, MAC is determined with the up-and-down method, whereas in animal the bracketing technique is commonly used. The objectives of this study were to determine the MAC value of isoflurane in dog ...
DOI:
10.1016/j.rvsc.2016.03.011

12. Differentiation at necropsy between in vivo gas embolism and putrefaction using a gas score

Author:
Bernaldo de Quirós, Yara; Saavedra, Pedro; Møllerløkken, Andreas; Brubakk, Alf O.; Jørgensen, Arve; González-Díaz, Oscar; Martín-Barrasa, Jose L.; Fernández, Antonio
Source:
Research in veterinary science 2016 v.106 pp. 48-55
ISSN:
0034-5288
Subject:
Ziphiidae; air; animal transport; chemical analysis; computed tomography; death; disease diagnosis; embolism; intravenous injection; marine mammals; models; necropsy; rabbits; topography
Abstract:
... Gas bubble lesions consistent with decompression sickness in marine mammals were described for the first time in beaked whales stranded in temporal and spatial association with military exercises. Putrefaction gas is a post-mortem artifact, which hinders the interpretation of gas found at necropsy. Gas analyses have been proven to help differentiating putrefaction gases from gases formed after hyp ...
DOI:
10.1016/j.rvsc.2016.03.007

13. Effect of Acarbose, Sitagliptin and combination therapy on blood glucose, insulin, and incretin hormone concentrations in experimentally induced postprandial hyperglycemia of healthy cats

Author:
Mori, Akihiro; Ueda, Kaori; Lee, Peter; Oda, Hitomi; Ishioka, Katsumi; Arai, Toshiro; Sako, Toshinori
Source:
Research in veterinary science 2016 v.106 pp. 131-134
ISSN:
0034-5288
Subject:
acarbose; blood glucose; cats; gastric inhibitory polypeptide; glucagon-like peptide 1; glucose; humans; hyperglycemia; hypoglycemic agents; ingestion; insulin; mechanism of action; patients; secretin; therapeutics
Abstract:
... Acarbose (AC) and Sitagliptin (STGP) are oral hypoglycemic agents currently used either alone or in conjunction with human diabetic (Type 2) patients. AC has been used with diabetic cats, but not STGP thus far. Therefore, the objective of this study was to determine the potential use of AC or STGP alone and in combination for diabetic cats, by observing their effect on short-term post-prandial ser ...
DOI:
10.1016/j.rvsc.2016.04.001

14. Effect of equilibration time on the motility and functional integrity of canine spermatozoa frozen in three different extenders

Author:
Belala, R.; Briand-Amirat, L.; Vinciguerra, L.; Tainturier, D.; Kaidi, R.; Thorin, C.; Michaud, S.; Anton, M.; Bencharif, D.
Source:
Research in veterinary science 2016 v.106 pp. 66-73
ISSN:
0034-5288
Subject:
DNA; acridine orange; acrosome; dogs; egg yolk; iodides; low density lipoprotein; semen; staining; thawing
Abstract:
... The present work aimed to assess the effect of equilibration time on post-thaw motility parameters of canine sperm frozen in three extenders: 6% low-density lipoproteins (LDL), 6% liposomes (LIPO), and 40% egg yolk plasma (EYP). A second experiment is aimed at evaluating the functional integrity of canine spermatozoa frozen in the three extenders at the best equilibration time found in the experim ...
DOI:
10.1016/j.rvsc.2016.03.010

15. Effects of inactivated porcine epidemic diarrhea virus on porcine monocyte-derived dendritic cells and intestinal dendritic cells

Author:
Gao, Qi; Zhao, Shanshan; Qin, Tao; Yin, Yinyan; Yu, Qinghua; Yang, Qian
Source:
Research in veterinary science 2016 v.106 pp. 149-158
ISSN:
0034-5288
Subject:
Porcine epidemic diarrhea virus; T-lymphocytes; adaptive immunity; dendritic cells; diarrhea; immune response; interferon-gamma; interleukin-12; intestines; mortality; piglets; vaccines; viral antigens
Abstract:
... Porcine epidemic diarrhea (PED) is a serious infection in neonatal piglets. As the causative agent of PED, porcine epidemic diarrhea virus (PEDV) results in acute diarrhea and dehydration with high mortality rates in swine. Dendritic cells (DCs) are highly effective antigen-presenting cells to uptake and present viral antigens to T cells, which then initiate a distinct immune response. In this stu ...
DOI:
10.1016/j.rvsc.2016.03.023

16. Epidemiological investigation reveals genetic diversity and high co-infection rate of canine bocavirus strains circulating in Heilongjiang province, Northeast China

Author:
Guo, Donghua; Wang, Zhihui; Yao, Shuang; Li, Chunqiu; Geng, Yufei; Wang, Enyu; Zhao, Xiwen; Su, Mingjun; Wei, Shan; Wang, Xinyu; Feng, Li; Chang, Yung-fu; Sun, Dongbo
Source:
Research in veterinary science 2016 v.106 pp. 7-13
ISSN:
0034-5288
Subject:
amino acids; dogs; epidemiological studies; feces; genes; genetic variation; mixed infection; nucleotide sequences; pathogenicity; phylogeny; polymerase chain reaction; China; Germany; South Korea; United States
Abstract:
... To trace evolution of CBoV in Northeast China, 201 fecal samples from rectal swabs of diarrheic dogs collected from May 2014 to April 2015 were investigated using PCR targeting partial NS1 gene (440bp). Furthermore, phylogenetic analysis of the identified CBoV strains was conducted using nucleotide sequences of the partial NS1 gene. The results indicated that 15 of 201 fecal samples (7.5%) were po ...
DOI:
10.1016/j.rvsc.2016.03.003
PubMed:
27234529
PubMed Central:
PMC7111776

17. Establishment and characterization of an MDCK cell line stably-transfected with chicken Abcb1 encoding P-glycoprotein

Author:
Sun, Yong; Guo, Tingting; Guo, Dawei; Guo, Li; Chen, Li; Zhang, Yu; Wang, Liping
Source:
Research in veterinary science 2016 v.106 pp. 37-44
ISSN:
0034-5288
Subject:
P-glycoproteins; bioavailability; cattle; chickens; complementary DNA; drug interactions; drugs; human cell lines; humans; models; nucleotides; open reading frames; pharmacokinetics; rats; sequence alignment; swine
Abstract:
... Chicken P-glycoprotein (chP-gp), encoded by Abcb1, determines the bioavailability because of its effect on pharmacokinetics of various drugs. However, comprehensive studies on chP-gp are still limited. In this study, the chicken full-length cDNA was first successfully cloned and then stably expressed in MDCK cell line. The open reading frame of chicken Abcb1 consists of 3864 nucleotides, encoding ...
DOI:
10.1016/j.rvsc.2016.03.004

18. Expression analysis of Sox9 genes during annual reproductive cycles in gonads and after nanodelivery of LHRH in Clarias batrachus

Author:
Bhat, Irfan Ahmad; Rather, Mohd Ashraf; Saha, Ratnadeep; Pathakota, Gireesh-Babu; Pavan-Kumar, Annam; Sharma, Rupam
Source:
Research in veterinary science 2016 v.106 pp. 100-106
ISSN:
0034-5288
Subject:
Clarias batrachus; amino acids; blood; brain; chitosan; complementary DNA; females; gene expression; genes; gonadotropin-releasing hormone; gonads; luteinization; males; messenger RNA; nanoparticles; nucleotide sequences; particle size; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; salmon; seasonal variation; spawning; tissues; transcription factors; zeta potential
Abstract:
... Transcription factor Sox9 plays a crucial role in determining the fate of several cell types and is a primary factor in regulation of gonadal development. Present study reports full-length cDNA sequence of Sox9a gene and partial coding sequence (cds) of Sox9b (two duplicate orthologs of Sox9 gene) from Clarias batrachus. The coding region of Sox9a gene encoded a peptide of 460 amino acids. The par ...
DOI:
10.1016/j.rvsc.2016.03.022

19. HER-2, EGFR, Cox-2 and Ki67 expression in lymph node metastasis of canine mammary carcinomas: Association with clinical-pathological parameters and overall survival

Author:
Araújo, M.R.; Campos, L.C.; Damasceno, K.A.; Gamba, C.O.; Ferreira, E.; Cassali, G.D.
Source:
Research in veterinary science 2016 v.106 pp. 121-130
ISSN:
0034-5288
Subject:
breast neoplasms; carcinoma; dogs; erbB-2 receptor; genetic markers; immunohistochemistry; lymph nodes; mammary neoplasms (animal); metastasis
Abstract:
... Studies about canine mammary tumors based on single molecular markers probably cannot accurately account for the heterogeneity of this disease, and the investigation of multiple molecular alterations in primary tumors and their metastases, in conjunction, has assumed great importance for the understanding of mammary tumor progression. In the present study, we selected 54 primary mammary carcinomas ...
DOI:
10.1016/j.rvsc.2016.03.020

20. Intraosseous stress distribution and bone interaction during load application across the canine elbow joint: A preliminary finite element analysis for determination of condylar fracture pathogenesis in immature and mature dogs

Author:
Böhme, Beatrice; d'Otreppe, Vinciane; Ponthot, Jean-Phillippe; Balligand, Marc
Source:
Research in veterinary science 2016 v.106 pp. 143-148
ISSN:
0034-5288
Subject:
dogs; finite element analysis; humeral fractures; models; pathogenesis; ulna
Abstract:
... Distal humeral fractures are common fractures especially in immature small breed dogs. The pathogenesis is still unknown. For this study, a three- dimensional bone model of the canine elbow was created and finite element analysis performed in order to determine the relationship between fracture type and bone interactions. Fused and non-fused humeral condyles were considered. A failure criterion wa ...
DOI:
10.1016/j.rvsc.2016.03.014