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1. Humanization of Yeast to Produce Complex Terminally Sialylated Glycoproteins

Author:
Li, Huijuan,   et al.   ; Hamilton, Stephen R.; Davidson, Robert C.; Sethuraman, Natarajan; Nett, Juergen H.; Jiang, Youwei; Rios, Sandra; Bobrowicz, Piotr; Stadheim, Terrance A.; Choi, Byung-Kwon; Hopkins, Daniel; Wischnewski, Harry; Roser, Jessica; Mitchell, Teresa; Strawbridge, Rendall R.; Hoopes, Jack; Wildt, Stefan; Gerngross, Tillman U.;     Show all 18 Authors
Source:
Science 2006 v.313 no.5792 pp. 1441-1443
ISSN:
0036-8075
Subject:
engineering; Komagataella pastoris; humans; glycosylation; recombinant proteins; yeasts; genes; erythropoietin
Abstract:
... Yeast is a widely used recombinant protein expression system. We expanded its utility by engineering the yeast Pichia pastoris to secrete human glycoproteins with fully complex terminally sialylated N-glycans. After the knockout of four genes to eliminate yeast-specific glycosylation, we introduced 14 heterologous genes, allowing us to replicate the sequential steps of human glycosylation. The rep ...
DOI:
10.1126/science.1130256