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1. Pharmacology and polyphenolic contents of in vitro propagated Aloe pruinosa Reynolds grown ex vitro

Author:
Baskaran, P.,   et al.   ; Gebashe, F.; Van Staden, J.;     Show all 3 Authors
Source:
South African journal of botany 2019 v.122 pp. 504-509
ISSN:
0254-6299
Subject:
Aloe; Staphylococcus aureus; acetylcholinesterase; antibacterial properties; enzyme inhibition; flavonoids; indole acetic acid; medicinal plants; micropropagation; pharmacology; phenolic compounds; pulp; seedlings; thidiazuron
Abstract:
... Analysis of the bioactivity of ex vitro-grown micropropagated plants of a vulnerable traditional medicinal plant, Aloe pruinosa Reynolds, is essential to promote the supplementation of wild resources. Two-year-old ex vitro-acclimatized plants of A. pruinosa, established using different plant growth regulators and greenhouse-grown in vitro and in vivo seedlings, were evaluated for their polyphenoli ...
DOI:
10.1016/j.sajb.2018.04.012

2. Ultrastructure of somatic embryo development and plant propagation for Lachenalia montana

Author:
Baskaran, P.,   et al.   ; Van Staden, J.;     Show all 2 Author
Source:
South African journal of botany 2017 v.109 pp. 269-274
ISSN:
0254-6299
Subject:
2,4-D; Golgi apparatus; Lachenalia; callus; cell suspension culture; chloroplasts; developmental stages; embryogenesis; explants; germination; germplasm conservation; greenhouses; histology; leaves; lipid bodies; mitochondria; plant propagation; plantlets; somatic embryos; starch granules; sucrose; thidiazuron; transmission electron microscopy; ultrastructure; vacuoles; viability
Abstract:
... Embryogenesis for plant propagation by cell suspension culture was achieved for the first time using friable embryogenic callus (FEC) from leaf explants of Lachenalia montana Schltr. ex W.F. Barker. FEC was established with solid (8gL−1 agar) MS (Murashige and Skoog, 1962) medium containing various concentrations and combinations of sucrose and plant growth regulators (PGRs). The somatic embryos ( ...
DOI:
10.1016/j.sajb.2017.01.006

3. Herbal medicine as a live practice for treating livestock ailments by indigenous people: A case study from the Konar community of Tamil Nadu

Author:
Baskaran, N.,   et al.   ; Jayakumar, S.; Arumugam, R.; Sathiskumar, S.; Pugazhenthi, M.;     Show all 5 Authors
Source:
South African journal of botany 2018 v.118 pp. 23-32
ISSN:
0254-6299
Subject:
Azadirachta indica; Bambusa bambos; Curcuma longa; Justicia adhatoda; Vitex negundo; Zingiber officinale; case studies; cattle; descriptive statistics; diarrhea; foot-and-mouth disease; herbal medicines; humans; indigenous knowledge; indigenous peoples; livelihood; medicinal plants; questionnaires; rural areas; traditional medicine; India
Abstract:
... Konar, an ethnic group of Yadav community, with cattle raising as its major occupation, is known for treating its cattle ailments using a wide range of traditional herbal medicines. The use of herbal medicine for human ailments has dwindled over the past few decades with the advent of allopathy, and if it is still being used as a viable practice for livestock ailment is not well known. The present ...
DOI:
10.1016/j.sajb.2018.06.002

4. In vitro regeneration of Begonia homonyma — A threatened plant

Author:
Baskaran, P.,   et al.   ; Kumari, A.; Van Staden, J.;     Show all 3 Authors
Source:
South African journal of botany 2017 v.109 pp. 174-177
ISSN:
0254-6299
Subject:
Begonia; benzyladenine; culture media; explants; gene banks; genetic transformation; gibberellic acid; in vitro regeneration; indole butyric acid; leaves; medicinal plants; naphthaleneacetic acid; necrosis; organogenesis; planting; plantlets; root growth; rooting; shoots; sucrose; threatened species
Abstract:
... An efficient simple in vitro plant regeneration system was established for Begonia homonyma, a threatened ornamental and medicinal plant. Shoot multiplication was improved significantly with a concomitant decrease in shoot length from leaf explants cultured on Murashige and Skoog (MS) medium containing 15μM benzyladenine (BA) and 5μM naphthaleneacetic acid (NAA). Shoots were established further in ...
DOI:
10.1016/j.sajb.2016.12.027

5. Rapid propagation of Mondia whitei by embryonic cell suspension culture in vitro

Author:
Baskaran, Ponnusamy,   et al.   ; Kumari, Aloka; Van Staden, Johannes;     Show all 3 Authors
Source:
South African journal of botany 2017 v.108 pp. 281-286
ISSN:
0254-6299
Subject:
2,4-D; Mondia whitei; artificial seeds; bioactive compounds; bioactive properties; calcium chloride; callus; cell suspension culture; explants; genetic transformation; germination; germplasm conservation; leaves; medicinal plants; naphthaleneacetic acid; picloram; plantlets; sodium alginate; somatic embryogenesis; somatic embryos; sucrose; vermiculite
Abstract:
... We have developed an efficient in vitro mass propagation system for Mondia whitei, an endangered South African medicinal plant of significant traditional importance, using somatic embryogenesis in cell suspension culture. Leaf explants generated friable embryogenic calli (FEC) when cultured on embryogenic callus induction medium (ECIM), which contained Murashige and Skoog (MS) medium, 8gL−1 agar w ...
DOI:
10.1016/j.sajb.2016.11.009

6. Optimization of transient GUS expression of Agrobacterium-mediated transformation in Dierama erectum Hilliard using sonication and Agrobacterium

Author:
Baskaran, P.,   et al.   ; Koetle, M.J.; Finnie, J.F.; Soos, V.; Balázs, E.; Van Staden, J.;     Show all 6 Authors
Source:
South African journal of botany 2017 v.111 pp. 307-312
ISSN:
0254-6299
Subject:
Agrobacterium; acetosyringone; apical meristems; bacteria; callus; coculture; explants; gene transfer; genetic transformation; geophytes; inoculum; polymerase chain reaction; transgenes; transgenic plants
Abstract:
... Efficient Agrobacterium-mediated transformation was established via sonication of embryonic shoot apical meristems (ESAMs) of Dierama erectum Hilliard. Effects of explant types, co-cultivation time, acetosyringone concentration, Agrobacterium concentration and different gene delivery methods were evaluated for higher efficiency of genetic transformation in D. erectum. An explant type (ESAMs), conc ...
DOI:
10.1016/j.sajb.2017.03.025

7. Shoot apical meristem injection: A novel and efficient method to obtain transformed cucumber plants

Author:
Baskaran, P.,   et al.   ; Soós, V.; Balázs, E.; Van Staden, J.;     Show all 4 Authors
Source:
South African journal of botany 2016 v.103 pp. 210-215
ISSN:
0254-6299
Subject:
Agrobacterium; Cucumis sativus; agar; apical meristems; cefotaxime; coculture; cucumbers; gene transfer; genetic transformation; germination; glucose; kanamycin; plant growth substances; plasmids; polymerase chain reaction; root growth; salts; shoots; transgenes; transgenic plants
Abstract:
... The objective of this study was to develop a simple and efficient system for the genetic transformation of cucumber (Cucumis sativus L.). This study investigated the factors influencing the efficiency of gene transfer by microinjection of the shoot apical meristem (SAM) with Agrobacterium shooter strain. The SAM microinjected with Agrobacterium cells containing binary plasmid was incubated in co-c ...
DOI:
10.1016/j.sajb.2015.09.006

8. Rapid in vitro micropropagation of Agapanthus praecox

Author:
Baskaran, Ponnusamy,   et al.   ; Van Staden, Johannes;     Show all 2 Author
Source:
South African journal of botany 2013 v.86 pp. 46-50
ISSN:
0254-6299
Subject:
Agapanthus; acclimation; benzyladenine; culture media; explants; genes; greenhouses; indole acetic acid; isopentenyladenine; micropropagation; rooting; roots; shoots; thidiazuron
Abstract:
... In vitro micropropagation and acclimatization for the ornamental Agapanthus praecox, are reported. The influence of different growth regulators on shoot multiplication from shoot-tip explants of A. praecox was investigated. Prolific shoot multiplication (47.3±1.96 shoots per explant) was achieved on Murashige and Skoog (MS) medium supplemented with 22.2μM benzyladenine (BA), 2.9μM indole-3-acetic ...
DOI:
10.1016/j.sajb.2013.01.008

9. In vitro plant regeneration, phenolic compound production and pharmacological activities of Coleonema pulchellum

Author:
Baskaran, P.,   et al.   ; Moyo, M.; Van Staden, J.;     Show all 3 Authors
Source:
South African journal of botany 2014 v.90 pp. 74-79
ISSN:
0254-6299
Subject:
acclimation; antibacterial properties; casein hydrolysates; cytokinins; elicitors; flavonoids; glutamine; hemoglobin; in vitro culture; indole acetic acid; medicinal properties; micropropagation; phenolic compounds; shoots; thidiazuron
Abstract:
... Effects of plant growth regulators (PGRs) and organic elicitors (OEs) on Coleonema pulchellum in vitro micropropagation, secondary product production and pharmacological activities were evaluated. In vitro, ex vitro and parental plants of C. pulchellum were investigated for their potential to produce phenolic and pharmacological compounds. Different morphogenic characteristics of shoots were obtai ...
DOI:
10.1016/j.sajb.2013.10.005