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- Author:
- Nakamura, Yasuhide; Imai, Ichiro; Tuji, Akihiro; Suzuki, Noritoshi
- Source:
- The journal of eukaryotic microbiology 2016 v.63 no.2 pp. 271-274
- ISSN:
- 1066-5234
- Subject:
- Protozoa; new species; Sea of Japan
- Abstract:
- ... A new phaeodarian species, characterized by the presence of long developed side branches recurved proximally and distally on the surface of its radial tube, was described as Auloscena pleuroclada. This new species was only collected from the layers below the 250 m depth in the Sea of Japan. They have never been found in the shallower layers (above 250 m) of this sea or in other investigated areas. ...
- DOI:
- 10.1111/jeu.12274
-
http://dx.doi.org/10.1111/jeu.12274
- Author:
- Paiva, Thiago da Silva; Shao, Chen; Fernandes, Noemi Mendes; Borges, Bárbara do Nascimento; Silva‐Neto, Inácio Domingos
- Source:
- The journal of eukaryotic microbiology 2016 v.63 no.2 pp. 247-261
- ISSN:
- 1066-5234
- Subject:
- Ciliophora; estuaries; interphase; morphogenesis; new subspecies; phylogeny; ribosomal DNA; rivers; Brazil
- Abstract:
- ... Interphase specimens, aspects of physiological reorganization and divisional morphogenesis were investigated in a strain of a hypotrichous ciliate highly similar to Urostyla grandis Ehrenberg, (type species of Urostyla), collected from a mangrove area in the estuary of the Paraíba do Sul river (Rio de Janeiro, Brazil). The results revealed that albeit interphase specimens match with the known morp ...
- DOI:
- 10.1111/jeu.12273
-
http://dx.doi.org/10.1111/jeu.12273
- Author:
- Guo, Fengguang; Zhang, Haili; Payne, Harold Ross; Zhu, Guan
- Source:
- The journal of eukaryotic microbiology 2016 v.63 no.2 pp. 233-246
- ISSN:
- 1066-5234
- Subject:
- Cryptosporidium parvum; fatty acids; fluorescent antibody technique; gene expression regulation; genes; long-chain-fatty-acid-CoA ligase; merozoites; parasites; plasma membrane; protein synthesis; proteins; sporozoites; vacuoles
- Abstract:
- ... Cryptosporidium parvum is unable to synthesize fatty acids de novo, but possesses three long‐chain fatty acyl‐CoA synthetase (CpACS) isoforms for activating fatty acids. We have recently shown that these enzymes could be targeted to kill the parasite in vitro and in vivo. Here, we demonstrated that the CpACS genes were differentially expressed during the parasite life cycle, and their proteins wer ...
- DOI:
- 10.1111/jeu.12272
- PubMed:
- 26411755
- PubMed Central:
- PMC4775295
-
http://dx.doi.org/10.1111/jeu.12272
- Author:
- Yurchenko, Vyacheslav; Kostygov, Alexei; Havlová, Jolana; Grybchuk‐Ieremenko, Anastasiia; Ševčíková, Tereza; Lukeš, Julius; Ševčík, Jan; Votýpka, Jan
- Source:
- The journal of eukaryotic microbiology 2016 v.63 no.2 pp. 198-209
- ISSN:
- 1066-5234
- Subject:
- humans; Herpetomonas; genotype; new subfamily; new species; Blattodea; Phytomonas
- Abstract:
- ... In this study, we surveyed six species of cockroaches, two synanthropic (i.e. ecologically associated with humans) and four wild, for intestinal trypanosomatid infections. Only the wild cockroach species were found to be infected, with flagellates of the genus Herpetomonas. Two distinct genotypes were documented, one of which was described as a new species, Herpetomonas tarakana sp. n. We also pro ...
- DOI:
- 10.1111/jeu.12268
-
http://dx.doi.org/10.1111/jeu.12268
- Author:
- Korosh, Travis; Jordan, Kelsey D.; Wu, Ja‐Shin; Yarlett, Nigel; Upmacis, Rita K.
- Source:
- The journal of eukaryotic microbiology 2016 v.63 no.2 pp. 153-161
- ISSN:
- 1066-5234
- Subject:
- Trichomonas vaginalis; arachidonic acid; cell death; docosahexaenoic acid; eicosapentaenoic acid; lipid metabolism; males; mass spectrometry; metronidazole; oxygen; parasites; salts; sodium; toxicity; trichomoniasis
- Abstract:
- ... Trichomonas vaginalis is a sexually transmitted parasite and, while it is often asymptomatic in males, the parasite is associated with disease in both sexes. Metronidazole is an effective treatment for trichomoniasis, but resistant strains have evolved and, thus, it has become necessary to investigate other possible therapies. In this study, we examined the effects of native and oxidized forms of ...
- DOI:
- 10.1111/jeu.12263
-
http://dx.doi.org/10.1111/jeu.12263
- Author:
- Ndzi, Edward S.; Asonganyi, Tazoacha; Nkinin, Mary Bello; Xiao, Lihua; Didier, Elizabeth S.; Bowers, Lisa C.; Nkinin, Stephenson W.; Kaneshiro, Edna S.
- Source:
- The journal of eukaryotic microbiology 2016 v.63 no.2 pp. 146-152
- ISSN:
- 1066-5234
- Subject:
- DNA; Encephalitozoon cuniculi; Encephalitozoon intestinalis; equipment; feces; field experimentation; genotype; humans; pathogens; species identification; volunteers; Cameroon
- Abstract:
- ... Several enteric microsporidia species have been detected in humans and other vertebrates and their identifications at the genotype level are currently being elucidated. As advanced methods, reagents, and disposal kits for detecting and identifying pathogens become commercially available, it is important to test them in settings other than in laboratories with “state‐of‐the‐art” equipment and well‐ ...
- DOI:
- 10.1111/jeu.12262
- PubMed:
- 26303263
- PubMed Central:
- PMC4767690
-
http://dx.doi.org/10.1111/jeu.12262
- Author:
- Wan, Qiang; Lin, Yongchao; Mao, Yixian; Yang, Yuqi; Li, Qiao; Zhang, Siwen; Jiang, Yanxue; Tao, Wei; Li, Wei
- Source:
- The journal of eukaryotic microbiology 2016 v.63 no.2 pp. 162-170
- ISSN:
- 1066-5234
- Subject:
- cities; drinking water; feces; genotype; human diseases; humans; internal transcribed spacers; microsporidiosis; parasites; pathogens; phylogeny; polymerase chain reaction; public health; swine; wastewater; water pollution; China
- Abstract:
- ... This study analyzed 563 fecal specimens of asymptomatic pigs from five cities of northeast China for the prevalence and genetic characteristics of Enterocytozoon bieneusi. The parasite was detected in 267 of 563 (47.4%) pigs by nested PCR of the ribosomal internal transcribed spacer (ITS). The differences in prevalence between preweaned (58.0%, 94/162) and growing pigs (39.6%, 114/288) and between ...
- DOI:
- 10.1111/jeu.12264
-
http://dx.doi.org/10.1111/jeu.12264
- Author:
- Tachibana, Hiroshi; Yanagi, Tetsuo; Feng, Meng; Bandara, K. B. Anura T.; Kobayashi, Seiki; Cheng, Xunjia; Hirayama, Kenji; Rajapakse, R. P. V. Jayanthe
- Source:
- The journal of eukaryotic microbiology 2016 v.63 no.2 pp. 171-180
- ISSN:
- 1066-5234
- Subject:
- Entamoeba; Macaca mulatta; abscess; body weight changes; feces; genes; hamsters; hexokinase; isozymes; phylogeny; polymerase chain reaction; ribosomal RNA; virulence; Sri Lanka
- Abstract:
- ... We have proposed the revival of the name Entamoeba nuttalli for a virulent ameba strain, P19‐061405, from a rhesus macaque and located it phylogenetically between E. histolytica and E. dispar. As E. nuttalli was originally described for an ameba found in a toque macaque in Sri Lanka, the prevalence and characteristics of Entamoeba species in wild toque macaques were examined. PCR analysis of 227 s ...
- DOI:
- 10.1111/jeu.12265
-
http://dx.doi.org/10.1111/jeu.12265
- Author:
- Adamska, Małgorzata
- Source:
- The journal of eukaryotic microbiology 2016 v.63 no.2 pp. 262-270
- ISSN:
- 1066-5234
- Subject:
- Acanthamoeba; genes; genotype; human diseases; nucleotide sequences; patients; people; phylogeny; ribosomal RNA; risk; surface water; Poland
- Abstract:
- ... Acanthamoeba genus is divided into 20 genotypes (T1–T20) on the basis of the gene encoding 18S rRNA sequence. Using of at least 2 kbp gene fragments is strongly recommended to identify new genotypes and 5% difference is commonly used as a criterion of new genotypes, however, this value is questionable. In this paper, Polish Acanthamoeba strains described earlier on the basis of ~850 bp Ami fragmen ...
- DOI:
- 10.1111/jeu.12275
-
http://dx.doi.org/10.1111/jeu.12275
- Author:
- Zadrobílková, Eliška; Smejkalová, Pavla; Walker, Giselle; Čepička, Ivan
- Source:
- The journal of eukaryotic microbiology 2016 v.63 no.2 pp. 181-197
- ISSN:
- 1066-5234
- Subject:
- Amoebida; DNA; actin; amphibians; crane flies; freshwater; genes; intestines; lifestyle; new species; nucleotide sequences; phylogeny; reptiles; ribosomal RNA; sediments; ultrastructure
- Abstract:
- ... The genus Rhizomastix is a poorly known group of amoeboid heterotrophic flagellates living as intestinal commensals of insects, amphibians or reptiles, and as inhabitants of organic freshwater sediments. Eleven Rhizomastix species have been described so far, but DNA sequences from only a single species have been published. Recently, phylogenetic analyses confirmed a previous hypothesis that the ge ...
- DOI:
- 10.1111/jeu.12266
-
http://dx.doi.org/10.1111/jeu.12266
- Author:
- Tikhonenkov, Denis V.; Janouškovec, Jan; Keeling, Patrick J.; Mylnikov, Alexander P.
- Source:
- The journal of eukaryotic microbiology 2016 v.63 no.2 pp. 220-232
- ISSN:
- 1066-5234
- Subject:
- electron microscopy; flagellum; freshwater; genes; habitats; microbodies; microsymbionts; microtubules; new species; nucleotide sequences; phylogeny; ribosomal RNA; salinity; ultrastructure
- Abstract:
- ... A small free‐living freshwater bacteriotrophic flagellate Neobodo borokensis n. sp. was investigated by electron microscopy and analysis of its SSU ribosomal RNA gene. This protist has paraxonemal rods of typical bodonid structure in the flagella, mastigonemes on the proximal part of the posterior flagellum, two nearly parallel basal bodies, a compact kinetoplast, and discoid mitochondrial cristae ...
- DOI:
- 10.1111/jeu.12271
-
http://dx.doi.org/10.1111/jeu.12271
- Author:
- Tyml, Tomáš; Kostka, Martin; Ditrich, Oleg; Dyková, Iva
- Source:
- The journal of eukaryotic microbiology 2016 v.63 no.2 pp. 210-219
- ISSN:
- 1066-5234
- Subject:
- actin; basins; habitats; phylogeny; ribosomal DNA; Arctic region
- Abstract:
- ... A new amoebozoan species, Vermistella arctica n. sp., is described from marine habitats in the central part of Svalbard archipelago. This is the first report on Arctic amoebae belonging to the genus Vermistella Moran and Anderson, 2007, the type species of which was described from the opposite pole of the planet. Psychrophily proved in the new strains qualifies the genus Vermistella as a bipolar t ...
- DOI:
- 10.1111/jeu.12270
-
http://dx.doi.org/10.1111/jeu.12270