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1. Rauvolfia serpentina N‐methyltransferases involved in ajmaline and Nβ‐methylajmaline biosynthesis belong to a gene family derived from γ‐tocopherol C‐methyltransferase

Author:
Cázares‐Flores, Paulo; Levac, Dylan; De Luca, Vincenzo
Source:
The plant journal 2016 v.87 no.4 pp. 335-342
ISSN:
0960-7412
Subject:
Escherichia coli; Rauvolfia serpentina; biochemical pathways; biosynthesis; enzyme activity; gamma-tocopherol; genes; indole alkaloids; messenger RNA; molecular cloning; monoterpenoids; recombinant proteins; roots; substrate specificity
Abstract:
... Ajmaline biosynthesis in Rauvolfia serpentina has been one of the most studied monoterpenoid indole alkaloid (MIA) pathways within the plant family Apocynaceae. Detailed molecular and biochemical information on most of the steps involved in the pathway has been generated over the last 30 years. Here we report the identification, molecular cloning and functional expression in Escherichia coli of tw ...
DOI:
10.1111/tpj.13186