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- Cázares‐Flores, Paulo; Levac, Dylan; De Luca, Vincenzo
- The plant journal 2016 v.87 no.4 pp. 335-342
- Escherichia coli; Rauvolfia serpentina; biochemical pathways; biosynthesis; enzyme activity; gamma-tocopherol; genes; indole alkaloids; messenger RNA; molecular cloning; monoterpenoids; recombinant proteins; roots; substrate specificity
- ... Ajmaline biosynthesis in Rauvolfia serpentina has been one of the most studied monoterpenoid indole alkaloid (MIA) pathways within the plant family Apocynaceae. Detailed molecular and biochemical information on most of the steps involved in the pathway has been generated over the last 30 years. Here we report the identification, molecular cloning and functional expression in Escherichia coli of tw ...