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You searched for: Journal The plant journal Remove constraint Journal: The plant journal Publication Year 2014 Remove constraint Publication Year: 2014 Source 2014 v.80 no.3 Remove constraint Source: 2014 v.80 no.3
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1. A dual‐color marker system for in vivo visualization of cell cycle progression in Arabidopsis

Author:
Yin, Ke; Ueda, Minako; Takagi, Hitomi; Kajihara, Takehiro; Sugamata Aki, Shiori; Nobusawa, Takashi; Umeda‐Hara, Chikage; Umeda, Masaaki
Source:
The plant journal 2014 v.80 no.3 pp. 541-552
ISSN:
0960-7412
Subject:
Arabidopsis; DNA replication; cells; cytokinesis; environmental factors; genes; histones; image analysis; mitosis; monitoring; replication origin
Abstract:
... Visualization of the spatiotemporal pattern of cell division is crucial to understand how multicellular organisms develop and how they modify their growth in response to varying environmental conditions. The mitotic cell cycle consists of four phases: S (DNA replication), M (mitosis and cytokinesis), and the intervening G1 and G2 phases; however, only G2/M‐specific markers are currently available ...
DOI:
10.1111/tpj.12652
PubMed:
25158977

2. Altered expression of Aurora kinases in Arabidopsis results in aneu‐ and polyploidization

Author:
Demidov, Dmitri; Lermontova, Inna; Weiss, Oda; Fuchs, Joerg; Rutten, Twan; Kumke, Katrin; Sharbel, Timothy F.; Van Damme, Daniel; De Storme, Nico; Geelen, Danny; Houben, Andreas
Source:
The plant journal 2014 v.80 no.3 pp. 449-461
ISSN:
0960-7412
Subject:
Arabidopsis; RNA interference; aneuploidy; chromosome segregation; meiosis; monitoring; mutants; phosphorylation; pollen; progeny; protein kinases; proteins; tetraploidy; tobacco; transfer DNA
Abstract:
... Aurora is an evolutionary conserved protein kinase family involved in monitoring of chromosome segregation via phosphorylation of different substrates. In plants, however, the involvement of Aurora proteins in meiosis and in sensing microtubule attachment remains to be proven, although the downstream components leading to the targeting of spindle assembly checkpoint signals to anaphase‐promoting c ...
DOI:
10.1111/tpj.12647
PubMed:
25146886

3. Arabidopsis inositol pentakisphosphate 2‐kinase, AtIPK1, is required for growth and modulates phosphate homeostasis at the transcriptional level

Author:
Kuo, Hui‐Fen; Chang, Tzu‐Yun; Chiang, Su‐Fen; Wang, Wei‐Di; Charng, Yee‐yung; Chiou, Tzyy‐Jen
Source:
The plant journal 2014 v.80 no.3 pp. 503-515
ISSN:
0960-7412
Subject:
Arabidopsis; biosynthesis; chromatin; genes; histones; homeostasis; mutants; mutation; myo-inositol; root growth; root systems; seeds; transcription (genetics); transcription factors; transcriptomics
Abstract:
... Inositol hexakisphosphate (IP₆) provides a phosphorous reservoir in plant seeds; in addition, along with its biosynthesis intermediates and derivatives, IP₆ also plays important roles in diverse developmental and physiological processes. Disruption of the Arabidopsis inositol pentakisphosphate 2‐kinase coding gene AtIPK1 was previously shown to reduce IP₆ content in vegetative tissues and affect p ...
DOI:
10.1111/tpj.12650
PubMed:
25155524

4. A stearoyl‐acyl carrier protein desaturase, NbSACPD‐C, is critical for ovule development in Nicotiana benthamiana

Author:
Zhang, Jiantao; Li, Jianhui; Garcia‐Ruiz, Hernan; Bates, Philip D.; Mirkov, Theodore Erik; Wang, Xiaofeng
Source:
The plant journal 2014 v.80 no.3 pp. 489-502
ISSN:
0960-7412
Subject:
Arabidopsis; Nicotiana benthamiana; abiotic stress; cell death; dwarfing; female fertility; genes; leaves; lipid composition; microRNA; ovules; phenotype; reciprocal crosses; small fruits; unsaturated fatty acids
Abstract:
... Stearoyl‐acyl carrier protein desaturase (SACPD) activity is essential for production of the major unsaturated fatty acids (UFAs) in plant lipids. We report here the characterization of three SACPD genes from Nicotiana benthamiana, NbSACPD‐A, ‐B, and ‐C. All three genes share high similarity to AtSSI2/FAB2 (Suppressor of Salicylic acid‐Insensitivity2/Fatty Acid Biosynthesis2), the primary SACPD is ...
DOI:
10.1111/tpj.12649
PubMed:
25155407

5. GFS9/TT9 contributes to intracellular membrane trafficking and flavonoid accumulation in Arabidopsis thaliana

Author:
Ichino, Takuji; Fuji, Kentaro; Ueda, Haruko; Takahashi, Hideyuki; Koumoto, Yasuko; Takagi, Junpei; Tamura, Kentaro; Sasaki, Ryosuke; Aoki, Koh; Shimada, Tomoo; Hara‐Nishimura, Ikuko
Source:
The plant journal 2014 v.80 no.3 pp. 410-423
ISSN:
0960-7412
Subject:
Arabidopsis thaliana; Golgi apparatus; color; endoplasmic reticulum; eukaryotic cells; flavonoids; flowers; genes; membrane fusion; membrane proteins; mutants; phenotype; physiological transport; phytochemicals; seeds; testa; vacuoles
Abstract:
... Flavonoids are the most important pigments for the coloration of flowers and seeds. In plant cells, flavonoids are synthesized by a multi‐enzyme complex located on the cytosolic surface of the endoplasmic reticulum, and they accumulate in vacuoles. Two non‐exclusive pathways have been proposed to mediate flavonoid transport to vacuoles: the membrane transporter‐mediated pathway and the vesicle tra ...
DOI:
10.1111/tpj.12637
PubMed:
25116949

6. High AN1 variability and interaction with basic helix‐loop‐helix co‐factors related to anthocyanin biosynthesis in potato leaves

Author:
D'Amelia, Vincenzo; Aversano, Riccardo; Batelli, Giorgia; Caruso, Immacolata; Castellano Moreno, Mar; Castro‐Sanz, Ana Beatriz; Chiaiese, Pasquale; Fasano, Carlo; Palomba, Francesca; Carputo, Domenico
Source:
The plant journal 2014 v.80 no.3 pp. 527-540
ISSN:
0960-7412
Subject:
Nicotiana tabacum; anthocyanins; biosynthesis; fluorescence; gene overexpression; leaves; loci; pigmentation; plant tissues; potatoes; promoter regions; regulator genes; stress tolerance; transcription factors; transgenic plants; tubers; yeasts
Abstract:
... AN1 is a regulatory gene that promotes anthocyanin biosynthesis in potato tubers and encodes a R2R3 MYB transcription factor. However, no clear evidence implicates AN1 in anthocyanin production in leaves, where these pigments might enhance environmental stress tolerance. In our study we found that AN1 displays intraspecific sequence variability in both coding/non‐coding regions and in the promoter ...
DOI:
10.1111/tpj.12653
PubMed:
25159050

7. HISTONE DEACETYLASE 9 represses seedling traits in Arabidopsis thaliana dry seeds

Author:
Zanten, Martijn; Zöll, Christian; Wang, Zhi; Philipp, Christina; Carles, Annaick; Li, Yong; Kornet, Noortje G.; Liu, Yongxiu; Soppe, Wim J. J.
Source:
The plant journal 2014 v.80 no.3 pp. 475-488
ISSN:
0960-7412
Subject:
Arabidopsis thaliana; acetylation; alleles; chromatin; data collection; germination; histone deacetylase; histones; imbibition; loci; mutants; phase transition; photosynthesis; precipitin tests; ribulose-bisphosphate carboxylase; ribulose-bisphosphate carboxylase activase; seed dormancy; seedlings; seeds; transcription (genetics); transcriptome
Abstract:
... Plant life is characterized by major phase changes. We studied the role of histone deacetylase (HDAC) activity in the transition from seed to seedling in Arabidopsis. Pharmacological inhibition of HDAC stimulated germination of freshly harvested seeds. Subsequent analysis revealed that histone deacetylase 9 (hda9) mutant alleles displayed reduced seed dormancy and faster germination than wild‐type ...
DOI:
10.1111/tpj.12646
PubMed:
25146719

8. Purification, molecular cloning and functional characterization of flavonoid C‐glucosyltransferases from Fagopyrum esculentum M. (buckwheat) cotyledon

Author:
Nagatomo, Yoshihisa; Usui, Shiori; Ito, Takamitsu; Kato, Akira; Shimosaka, Makoto; Taguchi, Goro
Source:
The plant journal 2014 v.80 no.3 pp. 437-448
ISSN:
0960-7412
Subject:
Escherichia coli; Fagopyrum esculentum; Oryza sativa; Zea mays; acid hydrolysis; buckwheat; carbon; complementary DNA; corn; cotyledons; flavonoids; genes; glycosyltransferases; isozymes; molecular cloning; phylogeny; proteins; rice; seed germination; seedlings; sugars
Abstract:
... C‐Glycosides are characterized by their C–C bonds in which the anomeric carbon of the sugar moieties is directly bound to the carbon atom of aglycon. C‐Glycosides are remarkably stable, as their C–C bonds are resistant to glycosidase or acid hydrolysis. A variety of plant species are known to accumulate C‐glycosylflavonoids; however, the genes encoding for enzymes that catalyze C‐glycosylation of ...
DOI:
10.1111/tpj.12645
PubMed:
25142187

9. Significant reduction of BiFC non‐specific assembly facilitates in planta assessment of heterotrimeric G‐protein interactors

Author:
Gookin, Timothy E.; Assmann, Sarah M.
Source:
The plant journal 2014 v.80 no.3 pp. 553-567
ISSN:
0960-7412
Subject:
Arabidopsis; Venus; cells; fluorescence; mutation; open reading frames; signal transduction
Abstract:
... Protein networks and signaling cascades are key mechanisms for intra‐ and intercellular signal transduction. Identifying the interacting partners of a protein can provide vital clues regarding its physiological role. The bimolecular fluorescence complementation (BiFC) assay has become a routine tool for in vivo analysis of protein–protein interactions and their subcellular location. Although the B ...
DOI:
10.1111/tpj.12639
PubMed:
25187041
PubMed Central:
PMC4260091

10. The gibberellin biosynthetic genes AtKAO1 and AtKAO2 have overlapping roles throughout Arabidopsis development

Author:
Regnault, Thomas; Davière, Jean‐Michel; Heintz, Dimitri; Lange, Theo; Achard, Patrick
Source:
The plant journal 2014 v.80 no.3 pp. 462-474
ISSN:
0960-7412
Subject:
Arabidopsis; cytochrome P-450; gene expression; genes; gibberellins; mutants; phenotype; plant development; quantitative analysis; seeds
Abstract:
... Ent‐kaurenoic acid oxidase (KAO), a class of cytochrome P450 monooxygenases of the subfamily CYP88A, catalyzes the conversion of ent‐kaurenoic acid (KA) to gibberellin (GA) GA₁₂, the precursor of all GAs, thereby playing an important role in determining GA concentration in plants. Past work has demonstrated the importance of KAO activity for growth in various plant species. In Arabidopsis, this en ...
DOI:
10.1111/tpj.12648
PubMed:
25146977

11. The movement of the non‐cell‐autonomous transcription factor, SHORT‐ROOT relies on the endomembrane system

Author:
Wu, Shuang; Gallagher, Kimberly L.
Source:
The plant journal 2014 v.80 no.3 pp. 396-409
ISSN:
0960-7412
Subject:
cells; endodermis; endomembrane system; endosomes; leaves; plasmodesmata; secretion; stele; transcription factors
Abstract:
... Plant cells are able to convey positional and developmental information between cells through the direct transfer of transcription factors. One well studied example of this is the SHORT‐ROOT (SHR) protein, which moves from the stele into the neighboring ground tissue layer to specify endodermis. While it has been shown that SHR trafficking relies on plasmodesmata (PD), and interaction with the SHR ...
DOI:
10.1111/tpj.12640
PubMed:
25124761

12. The Na⁺ transporter, TaHKT1;5‐D, limits shoot Na⁺ accumulation in bread wheat

Author:
Byrt, Caitlin Siobhan; Xu, Bo; Krishnan, Mahima; Lightfoot, Damien James; Athman, Asmini; Jacobs, Andrew Keith; Watson‐Haigh, Nathan S.; Plett, Darren; Munns, Rana; Tester, Mark; Gilliham, Matthew
Source:
The plant journal 2014 v.80 no.3 pp. 516-526
ISSN:
0960-7412
Subject:
Arabidopsis thaliana; DNA; RNA; Saccharomyces cerevisiae; Triticum aestivum; Xenopus laevis; genes; genetically modified organisms; leaves; loci; mesophyll; oocytes; plasma membrane; polymerase chain reaction; potassium; protoplasts; salt tolerance; shoots; sodium; stele; wheat; xylem vessels
Abstract:
... Bread wheat (Triticum aestivum L.) has a major salt tolerance locus, Kna1, responsible for the maintenance of a high cytosolic K⁺/Na⁺ ratio in the leaves of salt stressed plants. The Kna1 locus encompasses a large DNA fragment, the distal 14% of chromosome 4DL. Limited recombination has been observed at this locus making it difficult to map genetically and identify the causal gene. Here, we deciph ...
DOI:
10.1111/tpj.12651

13. UBC13, an E2 enzyme for Lys63‐linked ubiquitination, functions in root development by affecting auxin signaling and Aux/IAA protein stability

Author:
Wen, Rui; Wang, Sheng; Xiang, Daoquan; Venglat, Prakash; Shi, Xianzong; Zang, Yuepeng; Datla, Raju; Xiao, Wei; Wang, Hong
Source:
The plant journal 2014 v.80 no.3 pp. 424-436
ISSN:
0960-7412
Subject:
Arabidopsis; animals; enzyme activity; genes; indole acetic acid; lysine; mutants; phenotype; proteins; root hairs; ubiquitination; yeasts
Abstract:
... Unlike conventional lysine (K) 48–linked polyubiquitination, K63‐linked polyubiquitination plays signaling roles in yeast and animals. Thus far, UBC13 is the only known ubiquitin‐conjugating enzyme (E2) specialized in K63‐linked polyubiquitination. Previous identification of Arabidopsis genes encoding UBC13 as well as its interacting partner UEV1 indicates that the UBC13‐mediated ubiquitination pa ...
DOI:
10.1111/tpj.12644
PubMed:
25142088

14. Unexpected roles for ancient proteins: flavone 8‐hydroxylase in sweet basil trichomes is a Rieske‐type, PAO‐family oxygenase

Author:
Berim, Anna; Park, Jeong‐Jin; Gang, David R.
Source:
The plant journal 2014 v.80 no.3 pp. 385-395
ISSN:
0960-7412
Subject:
Ocimum basilicum; basil; catalysts; chloroplasts; databases; expressed sequence tags; flavones; genes; genetically engineered microorganisms; green fluorescent protein; leaves; metabolism; molecular cloning; oxygenases; peptides; trichomes
Abstract:
... Most elucidated hydroxylations in plant secondary metabolism are catalyzed by oxoglutarate‐ or cytochrome P450‐dependent oxygenases. Numerous hydroxylations still evade clarification, suggesting that they might be performed by alternative enzyme types. Here, we report the identification of the flavone 8‐hydroxylase (F8H) in sweet basil (Ocimum basilicum L.) trichomes as a Rieske‐type oxygenase. Se ...
DOI:
10.1111/tpj.12642
PubMed:
25139498