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- Singh, Suruchi; Sevalkar, Ritesh Rajesh; Sarkar, Dibyendu; Karthikeyan, Subramanian
- TheFEBS journal 2018 v.285 no.23 pp. 4424-4444
- DNA; DNA-directed RNA polymerase; Mycobacterium tuberculosis; amino acid sequences; crystal structure; dimerization; genes; mechanism of action; pathogenicity; pathogens; promoter regions; repressor proteins; sequence homology; transcription initiation
- ... The gene Rv1828 in Mycobacterium tuberculosis is shown to be essential for the pathogen and encodes for an uncharacterized protein. In this study, we have carried out biochemical and structural characterization of Rv1828 at the molecular level to understand its mechanism of action. The Rv1828 is annotated as helix‐turn‐helix (HTH)‐type MerR family transcription regulator based on its N‐terminal am ...
- Gahura, Ondřej; Panicucci, Brian; Váchová, Hana; Walker, John E.; Zíková, Alena
- TheFEBS journal 2018 v.285 no.23 pp. 4413-4423
- H-transporting ATP synthase; Trypanosoma brucei; adenosine triphosphate; amino acids; blood flow; cattle; dimerization; drugs; electron transport chain; enzyme inhibition; glycolysis; humans; hydrolysis; membrane potential; mitochondria; mitochondrial membrane; pH; parasites; protons; regulatory proteins; trypanosomiasis; yeasts
- ... Hydrolysis of ATP by the mitochondrial F‐ATPase is inhibited by a protein called IF₁. In the parasitic flagellate, Trypanosoma brucei, this protein, known as TbIF₁, is expressed exclusively in the procyclic stage, where the F‐ATPase is synthesizing ATP. In the bloodstream stage, where TbIF₁ is absent, the F‐ATPase hydrolyzes ATP made by glycolysis and compensates for the absence of a proton pumpin ...