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- Author:
- Ohmori, T.; Murata, M.; Motoyoshi, F.
- Source:
- Theoretical and applied genetics 1998 v.96 no.3-4 pp. 331-338
- ISSN:
- 0040-5752
- Subject:
- Arabidopsis; DNA; Southern blotting; Tomato mosaic virus; clones; disease resistance; genes; polymerase chain reaction; proteins; sequence homology; tomatoes
- Abstract:
- ... We examined near-isogenic lines (NILs) carrying either of the tomato mosaic virus (ToMV) resistance genes Tm-1 and Tm-2 for sequences homologous to the isolated disease-resistance genes. DNA fragments were amplified from the genomic DNA of the NILs by the polymerase chain reaction (PCR) using primers designed on the basis of sequences of certain domains conserved among some disease-resistance gene ...
- DOI:
- 10.1007/s001220050745
- PubMed:
- 24710868
-
http://dx.doi.org/10.1007/s001220050745
- Author:
- Samoylov, V.M.; Sink, K.C.
- Source:
- Theoretical and applied genetics 1996 v.92 no.7 pp. 850-857
- ISSN:
- 0040-5752
- Subject:
- Solanum lycopersicum var. lycopersicum; Solanum pennellii var. pennellii; Solanum melongena; somatic hybridization; protoplast fusion; gamma radiation; irradiation; dosage; hybrids; interspecific hybridization; intergeneric hybridization; mesophyll; protoplasts; DNA; flow cytometry; Southern blotting; nucleic acid hybridization
- Abstract:
- ... Highly asymmetric somatic hybrid plants were obtained by PEG/DMSO fusion of gamma-irradiated mesophyll protoplasts of the kanamycin-resistant (KmR+) interspecific hybrid Lycopersicon esculentum X L. pennellii (EP) with mesophyll protoplasts of Solanum melongena (eggplant, E). Elimination of the EP chromosomes was obtained by irradiating the donor genome with different doses of gamma rays (100, 250 ...
- DOI:
- 10.1007/BF00221897
- PubMed:
- 24166550
-
http://dx.doi.org/10.1007/BF00221897
- Author:
- Menke, U.; Schilde-Rentschler, L.; Ruoss, B.; Zanke, C.; Hemleben, V.; Ninnemann, H.
- Source:
- Theoretical and applied genetics 1996 v.92 no.5 pp. 617-626
- ISSN:
- 0040-5752
- Subject:
- Solanum tuberosum; tubers; genetic markers; mixoploidy; Southern blotting; hybrids; wild plants; somatic hybridization; DNA; DNA probes; interspecific hybridization; leaves; restriction fragment length polymorphism; protoplast fusion; electrofusion; chromosome number; tetraploidy; flow cytometry; Solanum pinnatisectum; plant morphology; breeding lines
- Abstract:
- ... Interspecific somatic hybrids between the 1EBN-wild species Solanum pinnatisectum (S. pnt) and four different diploid breeding lines of Solanum tuberosum (S. tbr) were produced by electrofusion. S. put exhibits resistance to Phytophthora infestans and Erwinia blackleg. Somatic hybrids were identified by RFLP analysis using the oligonucleotide (GATA)4 as a probe. In three of four combinations all r ...
- DOI:
- 10.1007/BF00224566
- PubMed:
- 24166331
-
http://dx.doi.org/10.1007/BF00224566
- Author:
- Lee, Y.O.; Kanno, A.; Kameya, T.
- Source:
- Theoretical and applied genetics 1996 v.92 no.1 pp. 10-14
- ISSN:
- 0040-5752
- Subject:
- Southern blotting; restriction fragment length polymorphism; cultivars; interspecific variation; Asparagus officinalis; restriction mapping; genome; DNA; DNA probes; Asparagus setaceus; Aspergillus; chloroplasts
- Abstract:
- ... The genus Asparagus consists of 100-300 species of both dioecious and hermaphrodite plants. Since there are diploid, tetraploid, and hexaploid plants in this genus, RFLP (restriction fragment length polymorphism) analysis of chloroplast DNA (ctDNA) is suitable for examining the phylogenetic relationships. We have constructed a physical map of the ctDNA of garden asparagus (A. officinalis L. cv 'Ma ...
- DOI:
- 10.1007/BF00222945
- PubMed:
- 24166110
-
http://dx.doi.org/10.1007/BF00222945
- Author:
- Peschke, V.M.; Phillips, R.L.; Gengenbach, B.G.
- Source:
- Theoretical and applied genetics 1991 v.82 no.2 pp. 121-129
- ISSN:
- 0040-5752
- Subject:
- Zea mays; transposons; somaclonal variation; tissue culture; DNA; DNA methylation; DNA probes; Southern blotting; restriction mapping; sequence homology
- Abstract:
- ... Our previous experiments on maize (Zea mays L.) plants regenerated from tissue culture revealed genetic activity characteristic of the transposable element Activator (Ac) in the progeny of 2-3% of the plants tested, despite the lack of Ac activity in the progenitor plants. The objective of the present study was to determine whether the presence of Ac activity in tissue-culture-derived plants was a ...
- DOI:
- 10.1007/BF00226202
- PubMed:
- 24213055
-
http://dx.doi.org/10.1007/BF00226202
- Author:
- Lijsebettens, M. van.; Vanderhaeghen, R.; Montagu, M. van.
- Source:
- Theoretical and applied genetics 1991 v.81 no.2 pp. 277-284
- ISSN:
- 0040-5752
- Subject:
- Arabidopsis thaliana; genetic transformation; Southern blotting; genetically modified organisms; insertional mutagenesis; loci; kanamycin; DNA; Agrobacterium radiobacter; genetic distance; drug resistance; genetic markers; recessive genes; linkage (genetics); plant morphology; leaves; tissue culture
- Abstract:
- ... We investigated the potential of the Agrobacterium tumefaciens T-DNA as an insertional mutagen in Arabidopsis thaliana. Arabidopsis lines transformed with different T-DNA vectors were generated using a leaf disc infection procedure adapted for efficient selection on either kanamycin or hygromycin medium. A standardized screening procedure was developed for the detection of recessive mutations in T ...
- DOI:
- 10.1007/BF00215734
- PubMed:
- 24221214
-
http://dx.doi.org/10.1007/BF00215734
- Author:
- Harada, T.; Sato, T.; Asaka, D.; Matsukawa, I.
- Source:
- Theoretical and applied genetics 1991 v.81 no.2 pp. 157-161
- ISSN:
- 0040-5752
- Subject:
- Oryza sativa; DNA; plastids; Southern blotting; restriction mapping; anther culture
- Abstract:
- ... Plastid DNA (ptDNA) in albino rice plants regenerated from pollen by anther culture was investigated by Southern blotting. Of the 20 albino plants investigated, 7 contained ptDNA that had suffered large-scale deletion. The size and location of the deletions differed among the plants. In all cases about 30 kbp of the region containing the PstI-2 fragment (15.7 kbp) had been retained. The deleted pt ...
- DOI:
- 10.1007/BF00215717
- PubMed:
- 24221197
-
http://dx.doi.org/10.1007/BF00215717
- Author:
- Brown, P.T.H.; Gobel, E.; Lorz, H.
- Source:
- Theoretical and applied genetics 1991 v.81 no.2 pp. 227-232
- ISSN:
- 0040-5752
- Subject:
- restriction fragment length polymorphism; Southern blotting; Zea mays; Oryza sativa; DNA; genes; somaclonal variation; sucrose synthase; callus; tissue culture
- Abstract:
- ... Tissue culture of the Zea mays inbred line A188 resulted in the regeneration of plants having a high level of phenotypic variation compared to seed-grown control plants. To determine how such variation was induced and whether this could be related to specific in vitro culture methods, callus cultures were established and maintained on different, commonly used culture media. Plants were regenerated ...
- DOI:
- 10.1007/BF00215727
- PubMed:
- 24221207
-
http://dx.doi.org/10.1007/BF00215727
- Author:
- Muehlbauer, G.J.; Staswick, P.E.; Specht, J.E.; Graef, G.L.; Shoemaker, R.C.; Keim, P.
- Source:
- Theoretical and applied genetics 1991 v.81 no.2 pp. 189-198
- ISSN:
- 0040-5752
- Subject:
- restriction fragment length polymorphism; Southern blotting; Glycine max; alleles; loci; DNA; chromosome mapping; linkage (genetics); genetic markers
- Abstract:
- ... A molecular marker analysis of a near-isogenic line (NIL), its donor parent (DP), and its recurrent parent (RP) can provide information about linkages between molecular markers and a conventional marker introgressed into the NIL. If the DP and RP possess different alleles for a given molecular marker, and if the NIL possesses the same allele as the DP, then it is reasonable to presume a linkage be ...
- DOI:
- 10.1007/BF00215722
- PubMed:
- 24221202
-
http://dx.doi.org/10.1007/BF00215722
- Author:
- Williams, M.E.; Hepburn, A.G.; Widholm, J.M.
- Source:
- Theoretical and applied genetics 1991 v.81 no.2 pp. 272-276
- ISSN:
- 0040-5752
- Subject:
- Zea mays; transposons; somaclonal variation; inbred lines; DNA; Southern blotting; tissue culture; mutants; mutation
- Abstract:
- ... Somaclonal variation (tissue culture-induced mutations) may result, in some instances, from the activation of transposable elements. This study was conducted to determine whether somaclonal variants in the Zea maize L. inbred line FR27rhm were associated with movement of the transposable element Activator (Ac). Ten variants, seven of which from genetic analyses fit a single recessive gene model an ...
- DOI:
- 10.1007/BF00215733
- PubMed:
- 24221213
-
http://dx.doi.org/10.1007/BF00215733
- Author:
- Graner, A.; Siedler, H.; Jahoor, A.; Herrmann, R.G.; Wenzel, G.
- Source:
- Theoretical and applied genetics 1990 v.80 no.6 pp. 826-832
- ISSN:
- 0040-5752
- Subject:
- restriction fragment length polymorphism; Southern blotting; restriction mapping; Hordeum vulgare; chromosomes; genome; DNA; probes (equipment); restriction endonucleases; Triticum aestivum; linkage (genetics); nucleic acid hybridization
- DOI:
- 10.1007/BF00224200
- PubMed:
- 24221117
-
http://dx.doi.org/10.1007/BF00224200
- Author:
- Dhar, M.S.; Pethe, V.V.; Gupta, V.S.; Ranjekar, P.K.
- Source:
- Theoretical and applied genetics 1990 v.80 no.3 pp. 402-408
- ISSN:
- 0040-5752
- Subject:
- Oryza sativa; cultivars; DNA; cytosine; adenine; methylation; restriction mapping; Southern blotting; nucleases; shoots; embryo (plant); repetitive sequences
- DOI:
- 10.1007/BF00210080
- PubMed:
- 24220977
-
http://dx.doi.org/10.1007/BF00210080