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- Author:
- Dhillon, Taniya; Stockinger, Eric J.
- Source:
- Theoretical and applied genetics 2013 v.126 no.11 pp. 2777-2789
- ISSN:
- 0040-5752
- Subject:
- DNA; barley; diploidy; frost; genes; genotype; germplasm; loci; polyploidy; substitution lines; wheat; winter hardiness
- Abstract:
- ... Freezing tolerance and winter hardiness are complex traits. In the Triticeae, two loci on the group 5 chromosome homoeologs are repeatedly identified as having major effects on these traits. Recently, we found that segments of the genomic region at one of these loci, Frost resistance-2 (Fr-2) is copy number variable in barley. Freezing-tolerant winter-hardy genotypes have greater tandem copy numbe ...
- DOI:
- 10.1007/s00122-013-2171-0
- PubMed:
- 23918064
-
http://dx.doi.org/10.1007/s00122-013-2171-0
- Author:
- Chen, Q.; Sun, S.; Ye, Q.; McCuine, S.; Huff, E.; Zhang, H.-B.
- Source:
- Theoretical and applied genetics 2004 v.108 no.6 pp. 1002-1009
- ISSN:
- 0040-5752
- Subject:
- DNA; DNA libraries; Leptinotarsa decemlineata; Phytophthora infestans; Solanum pinnatisectum; Verticillium wilt; bacterial artificial chromosomes; catechol oxidase; chloroplast genome; chromosome walking; clones; cyst nematodes; diploidy; disease resistance; genes; haploidy; insects; loci; molecular cloning; nucleotide sequences; pathogens; pest resistance; potatoes; restriction fragment length polymorphism; wild relatives
- Abstract:
- ... To facilitate isolation and characterization of disease and insect resistance genes important to potato, two bacterial artificial chromosome (BAC) libraries were constructed from genomic DNA of the Mexican wild diploid species, Solanum pinnatisectum, which carries high levels of resistance to the most important potato pathogen and pest, the late blight and the Colorado potato beetle (CPB). One of ...
- DOI:
- 10.1007/s00122-003-1513-8
-
http://dx.doi.org/10.1007/s00122-003-1513-8
- Author:
- Pattanavibool, R.; Aderkas, P. von.; Hanhijarvi, A.; Simola, L.K.; Bonga, J.M.
- Source:
- Theoretical and applied genetics 1995 v.90 no.5 pp. 671-674
- ISSN:
- 0040-5752
- Subject:
- Larix decidua; diploidy; chromosome number; embryo culture; embryo (plant); DNA; flow cytometry; haploidy; explants
- Abstract:
- ... Tested haploid embryogenic lines (n=12) of Larix dedicua Mill. initiated from megagametophyte tissue were maintained on half-strength LM medium without growth regulators. The cultures were analyzed for ploidy level after 1-9 years. All lines tested were found to have doubled (2n=24) their chromosome number at the end of the experiment, though there were a few lines that still gave occasional haplo ...
- DOI:
- 10.1007/BF00222132
- PubMed:
- 24174026
-
http://dx.doi.org/10.1007/BF00222132
- Author:
- Nakamura, Sunao; Hosaka, Kazuyoshi
- Source:
- Theoretical and applied genetics 2010 v.120 no.2 pp. 205-214
- ISSN:
- 0040-5752
- Subject:
- DNA; DNA methylation; diploidy; enzymes; gene expression; genes; heterosis; heterozygosity; homozygosity; inbred lines; inbreeding depression; potatoes; random amplified polymorphic DNA technique; selfing
- Abstract:
- ... Self-incompatible diploid potatoes were altered to self-compatible ones by a function of S-locus inhibitor gene and continued selfing generated highly homozygous inbreds. In this study, this process was investigated for the status of DNA methylation by a simple method using genomic DNA digested by methylation-sensitive restriction enzymes prior to RAPD analysis. We detected 31 methylation-sensitiv ...
- DOI:
- 10.1007/s00122-009-1058-6
- PubMed:
- 19455300
- PubMed Central:
- PMC2793386
-
http://dx.doi.org/10.1007/s00122-009-1058-6
- Author:
- Molnár, István; Kubaláková, Marie; Šimková, Hana; Farkas, András; Cseh, András; Megyeri, Mária; Vrána, Jan; Molnár-Láng, Márta; Doležel, Jaroslav
- Source:
- Theoretical and applied genetics 2014 v.127 no.5 pp. 1091-1104
- ISSN:
- 0040-5752
- Subject:
- Aegilops speltoides; DNA; DNA probes; Triticum urartu; breeding; chromosomes; diploidy; evolution; flow cytometry; fluorescence in situ hybridization; gene transfer; genes; genomics; hexaploidy; karyotyping; polymerase chain reaction; sorting; wheat; wild relatives
- Abstract:
- ... KEY MESSAGE : Chromosomes 5A ᵘ , 5S and 5D can be isolated from wild progenitors, providing a chromosome-based approach to develop tools for breeding and to study the genome evolution of wheat. The three subgenomes of hexaploid bread wheat originated from Triticum urartu (AᵘAᵘ), from a species similar to Aegilops speltoides (SS) (progenitor of the B genome), and from Ae. tauschii (DD). Earlier stu ...
- DOI:
- 10.1007/s00122-014-2282-2
- PubMed:
- 24553964
-
https://dx.doi.org/10.1007/s00122-014-2282-2
- Author:
- Riday, Heathcliffe; Krohn, Andrew L.
- Source:
- Theoretical and applied genetics 2010 v.121 no.4 pp. 761-767
- ISSN:
- 0040-5752
- Subject:
- DNA; Trifolium pratense; diploidy; gametophytes; genes; genetic markers; haplotypes; loci; microsatellite repeats; mutants; parents; plant breeding; pollen; progeny; random mating; testcrosses
- Abstract:
- ... Red clover is a hermaphroditic allogamous diploid (2n = 2x = 14) with a homomorphic gametophytic self-incompatibility (GSI) system (Trifolium pratense L.). Red clover GSI has long been studied, and it is thought that the genetic control of GSI constitutes a single locus. Although GSI genes have been identified in other species, the genomic location of the red clover GSI-locus remains unknown. The ...
- DOI:
- 10.1007/s00122-010-1347-0
- PubMed:
- 20461353
-
http://dx.doi.org/10.1007/s00122-010-1347-0
- Author:
- Chen, Xinwei; Lewandowska, Dominika; Armstrong, MilesR.; Baker, Katie; Lim, Tze-Yin; Bayer, Micha; Harrower, Brian; McLean, Karen; Jupe, Florian; Witek, Kamil; Lees, AlisonK.; Jones, JonathanD.; Bryan, GlennJ.; Hein, Ingo
- Source:
- Theoretical and applied genetics 2018 v.131 no.6 pp. 1287-1297
- ISSN:
- 0040-5752
- Subject:
- DNA; Phytophthora infestans; Solanum verrucosum; chromosome mapping; chromosomes; diploidy; disease resistance; parents; polymerase chain reaction; potatoes; resistance genes; single nucleotide polymorphism
- Abstract:
- ... KEY MESSAGE: A broad-spectrum late blight disease-resistance gene from Solanum verrucosum has been mapped to potato chromosome 9. The gene is distinct from previously identified-resistance genes. We have identified and characterised a broad-spectrum resistance to Phytophthora infestans from the wild Mexican species Solanum verrucosum. Diagnostic resistance gene enrichment (dRenSeq) revealed that t ...
- DOI:
- 10.1007/s00122-018-3078-6
- PubMed:
- 29560514
- PubMed Central:
- PMC5945768
-
http://dx.doi.org/10.1007/s00122-018-3078-6
- Author:
- Thyssen, Gregory N.; Fang, David D.; Turley, Rickie B.; Florane, Christopher; Li, Ping; Naoumkina, Marina
- Source:
- Theoretical and applied genetics 2014 v.127 no.10 pp. 2183-2192
- ISSN:
- 0040-5752
- Subject:
- DNA; Gossypium hirsutum; Gossypium raimondii; amino acid sequences; aquaporins; bioinformatics; chromosome mapping; cotton; diploidy; fiber cells; genes; genomics; high-throughput nucleotide sequencing; introgression; loci; mutants; phenotype; single nucleotide polymorphism; tetraploidy
- Abstract:
- ... KEY MESSAGE : Mapping-by-sequencing and novel subgenome-specific SNP markers were used to fine map the Ligon-lintless 2 ( Li ₂ ) short-fiber gene in tetraploid cotton. These methodologies will accelerate gene identification in polyploid species. Next generation sequencing offers new ways to identify the genetic mechanisms that underlie mutant phenotypes. The release of a reference diploid Gossypiu ...
- DOI:
- 10.1007/s00122-014-2372-1
- PubMed:
- 25119870
-
http://dx.doi.org/10.1007/s00122-014-2372-1
- Author:
- Nishikawa, Tomotaro; Vaughan, Duncan A.; Kadowaki, Koh-ichi
- Source:
- Theoretical and applied genetics 2005 v.110 no.4 pp. 696-705
- ISSN:
- 0040-5752
- Subject:
- DNA; Oryza; allopolyploidy; chloroplast genome; chloroplasts; diploidy; loci; microsatellite repeats; phylogeny; rice
- Abstract:
- ... Simple sequence repeats (SSR) and their flanking regions in the mitochondrial and chloroplast genomes were sequenced in order to reveal DNA sequence variation. This information was used to gain new insights into phylogenetic relationships among species in the genus Oryza. Seven mitochondrial and five chloroplast SSR loci equal to or longer than ten mononucleotide repeats were chosen from known ric ...
- DOI:
- 10.1007/s00122-004-1895-2
-
http://dx.doi.org/10.1007/s00122-004-1895-2
- Author:
- Massa, Alicia N.; Beecher, Brian; Morris, Craig F.
- Source:
- Theoretical and applied genetics 2007 v.114 no.7 pp. 1239-1247
- ISSN:
- 0040-5752
- Subject:
- Aegilops tauschii; DNA; Triticum monococcum; catechol oxidase; diploidy; durum wheat; gene duplication; hexaploidy; introns; loci; multigene family; noodles; phylogeny; sequence analysis; tetraploidy; wild relatives
- Abstract:
- ... Wheat polyphenol oxidase (PPO) is the major cause of browning reactions that discolor Asian noodles and other wheat products. It has been hypothesized that genes encoding wheat PPOs may have evolved by gene duplication into a multigene family. Here we characterized PPO genomic sequences from diploid (Triticum monococcum, T. urartu, Aegilops tauschii, and Ae. speltoides), tetraploid (T. turgidum, s ...
- DOI:
- 10.1007/s00122-007-0514-4
- PubMed:
- 17468807
-
http://dx.doi.org/10.1007/s00122-007-0514-4
- Author:
- Gulsen, Osman; Sever-Mutlu, Songul; Mutlu, Nedim; Tuna, Metin; Karaguzel, Osman; Shearman, Robert C.; Riordan, Terrance P.; Heng-Moss, Tiffany M.
- Source:
- Theoretical and applied genetics 2009 v.118 no.7 pp. 1309-1319
- ISSN:
- 0040-5752
- Subject:
- Cynodon; DNA; alleles; cultivars; diploidy; flow cytometry; genetic background; genetic markers; genetic polymorphism; genetic variation; geographical distribution; hexaploidy; microsatellite repeats; mountains; nuclear genome; random amplified polymorphic DNA technique; regression analysis; tetraploidy; triploidy; warm season grasses
- Abstract:
- ... Developing a better understanding of associations among ploidy level, geographic distribution, and genetic diversity of Cynodon accessions could be beneficial to bermudagrass breeding programs, and would enhance our understanding of the evolutionary biology of this warm season grass species. This study was initiated to: (1) determine ploidy analysis of Cynodon accessions collected from Turkey, (2) ...
- DOI:
- 10.1007/s00122-009-0982-9
- PubMed:
- 19229513
-
http://dx.doi.org/10.1007/s00122-009-0982-9
- Author:
- Gupta, Mehak; Gupta, Shilpa; Kumar, Hitesh; Kumar, Nitin; Banga, S. S.
- Source:
- Theoretical and applied genetics 2015 v.128 no.2 pp. 221-234
- ISSN:
- 0040-5752
- Subject:
- Brassica juncea; DNA; breeding value; diploidy; genetic variation; genome; genotype; germplasm; growth habit; heterosis; hybridization; hybrids; microsatellite repeats; population structure; prediction
- Abstract:
- ... KEY MESSAGE : Derived amphiploidy helped to resynthesize agronomically superior B. juncea germplasm which showed high heterosis in crosses with natural B. juncea . This new procedure facilitates a seamless flow of variation across Brassica digenomics. Brassica digenomics, artificially resynthesized by hybridizing extant genome donor diploids, show poor breeding value due to the linkage drag associ ...
- DOI:
- 10.1007/s00122-014-2423-7
- PubMed:
- 25399317
-
http://dx.doi.org/10.1007/s00122-014-2423-7
- Author:
- Kochert, G.; Halward, T.; Branch, W.D.; Simpson, C.E.
- Source:
- Theoretical and applied genetics 1991 v.81 no.5 pp. 565-570
- ISSN:
- 0040-5752
- Subject:
- restriction fragment length polymorphism; cultivars; diploidy; DNA; tetraploidy; genetic variation; Arachis hypogaea
- Abstract:
- ... RFLP variability was studied in eight U.S. peanut cultivars, representing the four market types, and in 14 wild Arachis species accessions, using random genomic clones from a PstI library. Very low levels of RFLP variability were found among the allotetraploids, which included the U.S. cultivars and Arachis monticola, a wild species. The diploid wild species were very diverse, however. RFLP patter ...
- DOI:
- 10.1007/BF00226719
- PubMed:
- 24221368
-
http://dx.doi.org/10.1007/BF00226719
- Author:
- Di Gaspero, Gabriele; Copetti, Dario; Coleman, Courtney; Castellarin, Simone Diego; Eibach, Rudolf; Kozma, Pál; Lacombe, Thierry; Gambetta, Gregory; Zvyagin, Andrey; Cindrić, Petar; Kovács, László; Morgante, Michele; Testolin, Raffaele
- Source:
- Theoretical and applied genetics 2012 v.124 no.2 pp. 277-286
- ISSN:
- 0040-5752
- Subject:
- DNA; Vitis; alleles; ancestry; breeding lines; diploidy; downy mildew; genotyping; grapes; haplotypes; humans; hybridization; loci; mildews; progeny; vines; France; Illinois; Missouri
- Abstract:
- ... The Rpv3 locus is a major determinant of downy mildew resistance in grapevine (Vitis spp.). A selective sweep at this locus was revealed by the DNA genotyping of 580 grapevines, which include a highly diverse set of 265 European varieties that predated the spread of North American mildews, 82 accessions of wild species, and 233 registered breeding lines with North American ancestry produced in the ...
- DOI:
- 10.1007/s00122-011-1703-8
- PubMed:
- 21947344
-
http://dx.doi.org/10.1007/s00122-011-1703-8
- Author:
- Akhunov, Eduard; Nicolet, Charles; Dvorak, Jan
- Source:
- Theoretical and applied genetics 2009 v.119 no.3 pp. 507-517
- ISSN:
- 0040-5752
- Subject:
- DNA; cultivars; diploidy; genetic variation; genotyping; hexaploidy; loci; polymerase chain reaction; single nucleotide polymorphism; tetraploidy; wheat
- Abstract:
- ... Single nucleotide polymorphisms (SNPs) are indispensable in such applications as association mapping and construction of high-density genetic maps. These applications usually require genotyping of thousands of SNPs in a large number of individuals. Although a number of SNP genotyping assays are available, most of them are designed for SNP genotyping in diploid individuals. Here, we demonstrate tha ...
- DOI:
- 10.1007/s00122-009-1059-5
- PubMed:
- 19449174
- PubMed Central:
- PMC2715469
-
http://dx.doi.org/10.1007/s00122-009-1059-5
