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- Author:
- Daub, L.; Geyer, A.; Reese, S.; Braun, J.; Otzdorff, C.
- Source:
- Theriogenology 2016 v.86 no.2 pp. 651-656
- ISSN:
- 0093-691X
- Subject:
- dogs; fluorescence; fluorescent dyes; microscopy; propidium; semen; sperm concentration; spermatozoa; staining; statistical analysis; viability
- Abstract:
- ... The objective of this study was to assess sperm membrane integrity in canine semen samples using three different vital stains and the NucleoCounter SP-100 (NC). In addition, the occurrence of half-stained sperm heads, the influence of investigator, and storage-related artifacts using stained smears were examined. Forty fresh (30 dogs) and 40 frozen–thawed (28 dogs) canine semen samples were analyz ...
- DOI:
- 10.1016/j.theriogenology.2016.02.021
-
https://dx.doi.org/10.1016/j.theriogenology.2016.02.021
- Author:
- Han, Xingfa; Li, Junli; Cao, Xiaohan; Du, Xiaogang; Meng, Fengyan; Zeng, Xianyin
- Source:
- Theriogenology 2016 v.86 no.2 pp. 657-665
- ISSN:
- 0093-691X
- Subject:
- adjuvants; blood sampling; blood serum; castration; feed conversion; growth performance; growth regulators; insulin-like growth factor I; liver; males; messenger RNA; ovalbumin; rats; somatoliberin; somatotropin; urea nitrogen; weight gain
- Abstract:
- ... Gonadotropin-inhibitory hormone (GnIH) is a potent positive regulator of the growth axis. The present study was aimed to comparatively investigate the effects of surgical and immunologic castration on hypothalamic GnIH expression and endocrine function of the growth axis. Thirty-six prepubertal male rats were randomly allocated into three groups (n = 12): control, surgically castrated or immunized ...
- DOI:
- 10.1016/j.theriogenology.2016.02.020
-
https://dx.doi.org/10.1016/j.theriogenology.2016.02.020
- Author:
- Honig, Hen; Ofer, Lior; Kaim, Moshe; Jacobi, Shamay; Shinder, Dima; Gershon, Eran
- Source:
- Theriogenology 2016 v.86 no.2 pp. 626-634
- ISSN:
- 0093-691X
- Subject:
- animal ovaries; blood flow; blood volume; cooling; dairy cows; estrous cycle; follicular development; heat stress; ovulation; progesterone; summer; ultrasonography
- Abstract:
- ... The use of ultrasound imaging for the examination of reproductive organs has contributed substantially to the fertility management of dairy cows around the world. This method has many advantages such as noninvasiveness and immediate availability of information. Adding Doppler index to the ultrasound imaging examination, improved the estimation of blood volume and flow rate to the ovaries in genera ...
- DOI:
- 10.1016/j.theriogenology.2016.02.017
-
https://dx.doi.org/10.1016/j.theriogenology.2016.02.017
- Author:
- Albers-Wolthers, C.H.J.; de Gier, J.; Rutten, V.P.M.G.; van Kooten, P.J.S.; Leegwater, P.A.J.; Schaefers-Okkens, A.C.; Kooistra, H.S.
- Source:
- Theriogenology 2016 v.86 no.2 pp. 589-595
- ISSN:
- 0093-691X
- Subject:
- Beagle; adults; agonists; anestrus; antagonists; bitches; body weight; contraception; estrus; gonadotropin-releasing hormone; intravenous injection; luteinizing hormone
- Abstract:
- ... Kisspeptin (KP) plays a key role in the regulation of the hypothalamic-pituitary-gonadal axis via the release of GnRH. As normal KP signaling is essential for reproductive function, it could be an interesting new target for therapeutic interventions, e.g., nonsurgical contraception in dogs. The aims of the present study were to investigate the effect of KP-10 administration on plasma LH concentrat ...
- DOI:
- 10.1016/j.theriogenology.2016.02.009
-
https://dx.doi.org/10.1016/j.theriogenology.2016.02.009