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1. An investigation of the microbiota in uterine flush samples and endometrial biopsies from dairy cows during the first 7 weeks postpartum

Author:
Knudsen, Lif Rødtness Vesterby; Karstrup, Cecilia Christensen; Pedersen, Hanne Gervi; Angen, Øystein; Agerholm, Jørgen Steen; Rasmussen, Eva Láadal; Jensen, Tim Kåre; Klitgaard, Kirstine
Source:
Theriogenology 2016 v.86 no.2 pp. 642-650
ISSN:
0093-691X
Subject:
Fusobacteriaceae; Holstein; Leptotrichiaceae; Mycoplasmataceae; biopsy; calving; dairy cows; endometritis; endometrium; genes; high-throughput nucleotide sequencing; metagenomics; microorganisms; pathogens; ribosomal RNA
Abstract:
... Metritis and endometritis commonly occur in dairy cows after calving. Although numerous studies have been performed to identify the causative pathogens, a complete overview has not been done. Metagenomic studies have analyzed the bacterial populations of uterine flush samples from postpartum (pp) dairy cows, but the microbiota in the uterine luminal fluid may differ from the microbiota of the endo ...
DOI:
10.1016/j.theriogenology.2016.02.016

2. A surgical glove port technique for laparoscopic-assisted ovariohysterectomy for pyometra in the bitch

Author:
Becher-Deichsel, Anja; Aurich, Jörg E.; Schrammel, Nadine; Dupré, Gilles
Source:
Theriogenology 2016 v.86 no.2 pp. 619-625
ISSN:
0093-691X
Subject:
animal ovaries; bitches; gloves; pyometra; spaying; umbilicus; uterus
Abstract:
... The objective of the study was to describe the feasibility of a glove port technique for laparoscopic-assisted surgical treatment of canine pyometra. In this retrospective case series, a total of 10 female dogs (median age 7 years, range 5.5–10.5 years; median weight 37.0 kg, range 12.9–64.0 kg) with pyometra were included. A multiaccess port was created from a surgical glove attached to an Alexis ...
DOI:
10.1016/j.theriogenology.2016.02.010

3. Aurora B inhibitor barasertib prevents meiotic maturation and subsequent embryo development in pig oocytes

Author:
Ju, Shiqiang; Peng, Xu; Yang, Xiaoliu; Sozar, Sparksi; Muneri, Caroline W.; Xu, Yaping; Chen, Changchao; Cui, Panpan; Xu, Weichao; Rui, Rong
Source:
Theriogenology 2016 v.86 no.2 pp. 503-515
ISSN:
0093-691X
Subject:
Western blotting; chromosome morphology; chromosomes; embryogenesis; extrusion; germinal vesicle; meiosis; metaphase; mitosis; staining; swine; telophase
Abstract:
... Barasertib, a highly selective Aurora B inhibitor, has been widely used in a variety of cells to investigate the role of Aurora B kinase, which has been implicated in various functions in the mitotic process. However, effects of barasertib on the meiotic maturation process are not fully understood, particularly in porcine oocyte meiotic maturation. In the present study, the effects of barasertib o ...
DOI:
10.1016/j.theriogenology.2016.01.030

4. Combination of oviduct fluid and heparin to improve monospermic zygotes production during porcine in vitro fertilization

Author:
Batista, Ribrio Ivan Tavares Pereira; Moro, Lucia N.; Corbin, Emilie; Alminana, Carmen; Souza-Fabjan, Joanna Maria Gonçalves; de Figueirêdo Freitas, Vicente José; Mermillod, Pascal
Source:
Theriogenology 2016 v.86 no.2 pp. 495-502
ISSN:
0093-691X
Subject:
embryogenesis; heparin; in vitro fertilization; oocytes; oviducts; polyspermy; pronucleus; proteins; sperm concentration; swine; zygote
Abstract:
... In vivo, the oviduct provides appropriate microenvironment conditions for monospermic fertilization and early embryo development. In addition, glycosaminoglycans such as heparin are present in the oviduct and have been shown to modulate the activity of oviduct-secreted proteins on the regulation of sperms parameters. Thus, the present study was designed to evaluate the effect of porcine oocytes ex ...
DOI:
10.1016/j.theriogenology.2016.01.031

5. Developmental competence and cryotolerance of caprine parthenogenetic embryos cultured in chemically defined media

Author:
Choi, Woo-Jae; Lee, Ji-Hyun; Lee, Sang-Hee; Yum, Soo-Young; Lee, Song-Jeon; Lim, Chae-Woong; Jang, Goo
Source:
Theriogenology 2016 v.86 no.2 pp. 596-603
ISSN:
0093-691X
Subject:
Capra hircus; animal reproduction; apoptosis; blastocyst; calcium; cryopreservation; culture media; embryo culture; fetal bovine serum; freezing; gene expression; goats; oocytes; oviducts; parthenogenesis; thawing; tissue culture
Abstract:
... In animal reproduction technologies, the in vitro embryo culture system has advanced over the past few decades. However, in vitro cultured embryos still have reduced functional and physiological abilities compared with those from in vivo conditions, and many factors of oviduct and uterine environments have not yet been revealed. Here, we demonstrated the in vitro culture of domestic goat (Capra hi ...
DOI:
10.1016/j.theriogenology.2016.02.012

6. Effect of antifreeze glycoprotein 8 supplementation during vitrification on the developmental competence of bovine oocytes

Author:
Liang, Shuang; Yuan, Bao; Kwon, Jeong-Woo; Ahn, Mija; Cui, Xiang-Shun; Bang, Jeong Kyu; Kim, Nam-Hyung
Source:
Theriogenology 2016 v.86 no.2 pp. 485-494.e1
ISSN:
0093-691X
Subject:
DNA damage; actin; antifreeze proteins; apoptosis; blastocyst; caspases; cathepsin B; cattle; chilling injury; chromosomes; cytoskeleton; embryogenesis; energy; meiosis; mitochondria; oocytes; protective effect; reactive oxygen species; vitrification
Abstract:
... The purpose of this study was to investigate the effect of antifreeze glycoprotein 8 (AFGP8) supplementation during vitrification on the survival, fertilization, and embryonic development of bovine oocytes and the underlying molecular mechanism(s). Survival, fertilization, early embryonic development, apoptosis, DNA double-strand breaks, reactive oxygen species levels, meiotic cytoskeleton assembl ...
DOI:
10.1016/j.theriogenology.2016.01.032

7. Effects of cryopreservation on sperm viability, synthesis of reactive oxygen species, and DNA damage of bovine sperm

Author:
Gürler, H.; Malama, E.; Heppelmann, M.; Calisici, O.; Leiding, C.; Kastelic, J.P.; Bollwein, H.
Source:
Theriogenology 2016 v.86 no.2 pp. 562-571
ISSN:
0093-691X
Subject:
DNA; DNA damage; DNA fragmentation; bulls; computers; cryopreservation; flow cytometry; hydrogen peroxide; membrane potential; mitochondria; mitochondrial membrane; spermatozoa; viability
Abstract:
... The objective was to examine if there are relationships between alterations in sperm viability, reactive oxygen species (ROS) synthesis, and DNA integrity induced by cryopreservation of bovine sperm. Four ejaculates were collected from each of six bulls. Each ejaculate was diluted and divided into two aliquots; one was incubated for 24 hours at 37 °C, and the other frozen, thawed, and incubated fo ...
DOI:
10.1016/j.theriogenology.2016.02.007

8. Establishment of conditions for ovum pick up and IVM of jennies oocytes toward the setting up of efficient IVF and in vitro embryos culture procedures in donkey (Equus asinus)

Author:
Goudet, Ghylène; Douet, Cécile; Kaabouba-Escurier, Aurore; Couty, Isabelle; Moros-Nicolás, Carla; Barrière, Philippe; Blard, Thierry; Reigner, Fabrice; Deleuze, Stefan; Magistrini, Michèle
Source:
Theriogenology 2016 v.86 no.2 pp. 528-535
ISSN:
0093-691X
Subject:
adults; asses; breeds; cryopreservation; culture media; epidermal growth factor; females; fetal bovine serum; genome; gentamicin; germinal vesicle; horses; in vitro fertilization; males; metaphase; parthenogenesis; procaine; pronucleus; semen; spermatozoa; tissue culture; zygote
Abstract:
... Most wild and domestic donkey breeds are currently endangered or threatened. Their preservation includes the creation of a Genome Resource Bank. Embryos cryopreservation allows the preservation of genetics from both male and female and is the fastest method to restore a breed. Because embryo production in vivo is limited in equids, our objective was to establish conditions for in vitro production ...
DOI:
10.1016/j.theriogenology.2016.02.004

9. Expression and function of transient receptor potential channels in the female bovine reproductive tract

Author:
Ghavideldarestani, Maryam; Atkin, Stephen L.; Leese, Henry J.; Sturmey, Roger G.
Source:
Theriogenology 2016 v.86 no.2 pp. 551-561
ISSN:
0093-691X
Subject:
calcium; cattle; epithelial cells; epithelium; estradiol; estrous cycle; females; follicle-stimulating hormone; gene expression; homeostasis; humans; ions; luteinizing hormone; models; oviducts; progesterone; protein synthesis; transient receptor potential channels
Abstract:
... The epithelium lining the oviduct is critical for early reproductive events, many of which are mediated via intracellular calcium ions. Despite this, little is known about the regulation of calcium homeostasis in the oviductal epithelium. Epithelial transient receptor potential channels (TRPCs) modulate calcium flux in other tissues, and their expression and functional regulation have therefore be ...
DOI:
10.1016/j.theriogenology.2016.02.005

10. Expression of androgen-producing enzyme genes and testosterone concentration in Angus and Nellore heifers with high and low ovarian follicle count

Author:
Loureiro, Bárbara; Ereno, Ronaldo L.; Favoreto, Mauricio G.; Barros, Ciro M.
Source:
Theriogenology 2016 v.86 no.2 pp. 523-527
ISSN:
0093-691X
Subject:
Angus; Nellore; enzymes; follicular fluid; gene expression; genes; heifers; messenger RNA; oocytes; testosterone; zebu
Abstract:
... Follicle population is important when animals are used in assisted reproductive programs. Bos indicus animals have more follicles per follicular wave than Bos taurus animals. On the other hand, B taurus animals present better fertility when compared with B indicus animals. Androgens are positively related with the number of antral follicles; moreover, they increase growth factor expression in gran ...
DOI:
10.1016/j.theriogenology.2016.02.001

11. Expression of cumulus-oocyte complex genes and embryonic development in goats subjected to progestogen-based estrus synchronization

Author:
Thammasiri, Jiratti; Navanukraw, Chainarong; Uriyapongson, Suthipong; Khanthusaeng, Vilaivan; Kamollirt, Chuchat
Source:
Theriogenology 2016 v.86 no.2 pp. 612-618
ISSN:
0093-691X
Subject:
B-lymphocytes; blastocyst; controlled internal drug release devices; embryogenesis; equine chorionic gonadotropin; estrus synchronization; follicular development; gene expression; genes; goats; in vitro fertilization; lymphoma; medroxyprogesterone; messenger RNA; morula; oocytes; ovariectomy; progesterone; Spain
Abstract:
... The objective was to investigate the effect of short-term (7 days) and long-term (14 days) progesterone-based estrus synchronization on number of follicles, progesterone concentrations, cumulus-oocyte complex (COC) gene expression, and embryonic development in goats. Nulliparous Thai-native goats (n = 45) were randomly assigned to one of two estrus synchronization treatments. Goats were treated wi ...
DOI:
10.1016/j.theriogenology.2016.02.011

12. Expression of 3β-hydroxysteroid dehydrogenase in ovarian and uterine tissue during diestrus and open cervix cystic endometrial hyperplasia-pyometra in the bitch

Author:
Gultiken, Nilgun; Yarim, Murat; Yarim, Gul Fatma; Gacar, Ayhan; Mason, James Ian
Source:
Theriogenology 2016 v.86 no.2 pp. 572-578
ISSN:
0093-691X
Subject:
animal ovaries; bitches; cervix; diestrus; endometrium; epithelium; humans; hyperplasia; progesterone; pyometra; rabbits
Abstract:
... The purpose of this study was to compare the expression of 3β-hydroxystreroid dehydrogenase (3β-HSD) in the uterus and ovary of healthy dogs and those with cystic endometrial hyperplasia and/or pyometra complex (CEH-pyometra). Eighteen female dogs were included in the study. Eleven bitches with open cervix CEH-pyometra were included in the CEH-pyometra group and seven diestrus bitches in the contr ...
DOI:
10.1016/j.theriogenology.2016.02.006

13. Extending the duration of treatment with progesterone and equine chorionic gonadotropin improves fertility in suckled beef cows with low body condition score subjected to timed artificial insemination

Author:
Bilbao, M.G.; Massara, N.; Ramos, S.; Zapata, L.O.; Farcey, M.F.; Pesoa, J.; Turic, E.; Vázquez, M.I.; Bartolome, J.A.
Source:
Theriogenology 2016 v.86 no.2 pp. 536-544
ISSN:
0093-691X
Subject:
artificial insemination; beef cows; body condition; equine chorionic gonadotropin; estradiol; follicular development; group effect; intravaginal devices; pregnancy; progesterone
Abstract:
... The objective of this study was to evaluate the effect of an extended progesterone treatment on follicular development and fertility in postpartum, suckled beef cows subjected to timed artificial insemination (TAI). In experiment 1, cows (n = 24) with body condition score (BCS) ≥4.5 received either a 2-g progesterone intravaginal device on Day −23 or a 0.558-g progesterone intravaginal device on D ...
DOI:
10.1016/j.theriogenology.2016.02.003

14. Functional angiocoupling between follicles and adjacent corpus luteum in heifers

Author:
Ginther, O.J.; Siddiqui, M.A.R.; Baldrighi, J.M.
Source:
Theriogenology 2016 v.86 no.2 pp. 465-471
ISSN:
0093-691X
Subject:
adverse effects; blood flow; color; corpus luteum; heifers; luteolysis; ovulation
Abstract:
... In single ovulating cattle, ipsilateral versus contralateral interovarian relationships refer to a dominant follicle (DF) and CL in the same versus opposite ovaries. The ipsilateral relationship consists of the DF−CL and the devoid (no DF or CL) intraovarian pattern, and the contralateral relationship consists of the DF pattern and the CL pattern. The DF–CL pattern involves positive effects on bot ...
DOI:
10.1016/j.theriogenology.2016.03.002

15. Inflammatory response in chronic degenerative endometritis mares treated with platelet-rich plasma

Author:
Reghini, Maria Fernanda S.; Ramires Neto, Carlos; Segabinazzi, Lorenzo G.; Castro Chaves, Maria Manoela B.; Dell'Aqua, Camila de Paula F.; Bussiere, Maria Clara C.; Dell'Aqua, José Antonio; Papa, Frederico O.; Alvarenga, Marco Antonio
Source:
Theriogenology 2016 v.86 no.2 pp. 516-522
ISSN:
0093-691X
Subject:
artificial insemination; cross-over studies; endometritis; endometrium; experimental design; histology; immunomodulators; inflammation; mares; medicine; neutrophils; nitric oxide; semen; sports; surgery; ultrasonography
Abstract:
... Degenerative changes of the endometrium are directly related to age and fertility in mares. Chronic degenerative endometritis (CDE) is correlated with uterine fluid retention and reduced ability to clear uterine inflammation. Recent research in the areas of equine surgery and sports medicine has shown that platelet-rich plasma (PRP) treatment acts as an immunomodulator of the inflammatory response ...
DOI:
10.1016/j.theriogenology.2016.01.029

16. Lack of transmission of murine norovirus to mice via in vitro fertilization, intracytoplasmic sperm injection, and ovary transplantation

Author:
Raspa, Marcello; Mahabir, Esther; Fray, Martin; Volland, Ruth; Scavizzi, Ferdinando
Source:
Theriogenology 2016 v.86 no.2 pp. 579-588
ISSN:
0093-691X
Subject:
Norovirus; animal ovaries; brain; embryo transfer; genome; germplasm; glutathione; intracytoplasmic sperm injection; liver; lymph nodes; mice; pups; reverse transcriptase polymerase chain reaction; risk; small intestine; spermatozoa; spleen; testes; zona pellucida
Abstract:
... Since its discovery in 2003, murine norovirus (MNV) is still endemic in many rodent animal facilities. Our aim was to determine the risk of transmission of MNV (91% homology to MNV3) to embryo recipients and pups via assisted reproductive technologies, especially those which compromise the integrity of the zona pellucida. In vitro fertilization (IVF), assisted in vitro fertilization (AIVF) with re ...
DOI:
10.1016/j.theriogenology.2016.02.008

17. Lipidome signatures in early bovine embryo development

Author:
Sudano, Mateus J.; Rascado, Tatiana D.S.; Tata, Alessandra; Belaz, Katia R.A.; Santos, Vanessa G.; Valente, Roniele S.; Mesquita, Fernando S.; Ferreira, Christina R.; Araújo, João P.; Eberlin, Marcos N.; Landim-Alvarenga, Fernanda D.C.
Source:
Theriogenology 2016 v.86 no.2 pp. 472-484.e1
ISSN:
0093-691X
Subject:
biomarkers; blastocyst; cattle; droplets; embryogenesis; environmental factors; fatty acids; gene overexpression; genes; lipid composition; messenger RNA; morula; oocytes; phospholipids; quantitative polymerase chain reaction; staining
Abstract:
... Mammalian preimplantation embryonic development is a complex, conserved, and well-orchestrated process involving dynamic molecular and structural changes. Understanding membrane lipid profile fluctuation during this crucial period is fundamental to address mechanisms governing embryogenesis. Therefore, the aim of the present work was to perform a comprehensive assessment of stage-specific lipid pr ...
DOI:
10.1016/j.theriogenology.2016.03.025

18. Production of feline leukemia inhibitory factor with biological activity in Escherichia coli

Author:
Kanegi, R.; Hatoya, S.; Tsujimoto, Y.; Takenaka, S.; Nishimura, T.; Wijewardana, V.; Sugiura, K.; Takahashi, M.; Kawate, N.; Tamada, H.; Inaba, T.
Source:
Theriogenology 2016 v.86 no.2 pp. 604-611
ISSN:
0093-691X
Subject:
Escherichia coli; bioactive properties; cats; complementary DNA; embryogenesis; embryonic stem cells; erythrocytes; feline leukemia; follicle-stimulating hormone; humans; leukemia inhibitory factor; metaphase; mice; neoplasm cells; oocytes; reproduction
Abstract:
... Leukemia inhibitory factor (LIF) is a cytokine which is essential for oocyte and embryo development, embryonic stem cell, and induced pluripotent stem cell maintenance. Leukemia inhibitory factor improves the maturation of oocytes in the human and the mouse. However, feline LIF (fLIF) cloning and effects on oocytes during IVM have not been reported. Thus, we cloned complete cDNA of fLIF and examin ...
DOI:
10.1016/j.theriogenology.2016.02.013

19. Progesterone levels and days to luteolysis in mares treated with intrauterine fractionated coconut oil

Author:
Diel de Amorim, Mariana; Nielsen, Kayla; Cruz, Raissa Karolliny Salgueiro; Card, Claire
Source:
Theriogenology 2016 v.86 no.2 pp. 545-550
ISSN:
0093-691X
Subject:
analysis of variance; artificial insemination; blood serum; coconut oil; computer software; diestrus; dose response; embryo transfer; estrus; intrauterine administration; longevity; luteolysis; mares; ovulation; progesterone; ultrasonography; uterus
Abstract:
... Intrauterine plant oil infusion, including fractionated coconut oil, has been previously found to be a safe, inexpensive, and reversible method of prolonging the luteal phase in mares when administered on Day 10 of the estrous cycle. Our objective was to understand the uteroovarian response to the administration of fractionated coconut oil infusion in the uterus of diestrous mares. We hypothesized ...
DOI:
10.1016/j.theriogenology.2016.02.002

20. Role of cumulus cells during vitrification and fertilization of mature bovine oocytes: Effects on survival, fertilization, and blastocyst development

Author:
Ortiz-Escribano, N.; Smits, K.; Piepers, S.; Van den Abbeel, E.; Woelders, H.; Van Soom, A.
Source:
Theriogenology 2016 v.86 no.2 pp. 635-641
ISSN:
0093-691X
Subject:
blastocyst; cattle; dimethyl sulfoxide; embryogenesis; ethylene glycol; fertilizer rates; oocytes; parthenogenesis; sucrose; survival rate; vitrification
Abstract:
... This study was designed to determine the role of cumulus cells during vitrification of bovine oocytes. Mature cumulus-oocyte complexes (COCs) with many layers of cumulus cells, corona radiata oocytes (CRs), with a few layers of cumulus cells, and denuded oocytes (DOs) without cumulus cells were vitrified in 15% ethylene glycol, 15% dimethyl sulfoxide, and 0.5-M sucrose. Oocytes that survived the v ...
DOI:
10.1016/j.theriogenology.2016.02.015