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You searched for: Journal Transgenic research Remove constraint Journal: Transgenic research Publication Year 2018 Remove constraint Publication Year: 2018 Source 2018 v.27 no.4 Remove constraint Source: 2018 v.27 no.4
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1. CRISPR/Cas9 editing of carotenoid genes in tomato

Author:
D’Ambrosio, Caterina; Stigliani, Adriana Lucia; Giorio, Giovanni
Source:
Transgenic research 2018 v.27 no.4 pp. 367-378
ISSN:
0962-8819
Subject:
DNA; alleles; animals; biosynthesis; breeding programs; carotenoids; color; diet; exons; flowers; fruits; gene editing; loci; loss-of-function mutation; phenotype; plant breeding; secondary metabolites; tomatoes
Abstract:
... CRISPR/Cas9 technology is rapidly spreading as genome editing system in crop breeding. The efficacy of CRISPR/Cas9 in tomato was tested on Psy1 and CrtR-b2, two key genes of carotenoid biosynthesis. Carotenoids are plant secondary metabolites that must be present in the diet of higher animals because they exert irreplaceable functions in important physiological processes. Psy1 and CrtR-b2 were cho ...
DOI:
10.1007/s11248-018-0079-9

2. Expression of disease resistance in genetically modified grapevines correlates with the contents of viral sequences in the T-DNA and global genome methylation

Author:
Dal Bosco, Daniela; Sinski, Iraci; Ritschel, Patrícia S.; Camargo, Umberto A.; Fajardo, Thor V. M.; Harakava, Ricardo; Quecini, Vera
Source:
Transgenic research 2018 v.27 no.4 pp. 379-396
ISSN:
0962-8819
Subject:
Botrytis cinerea; DNA methylation; Grapevine leafroll-associated virus 3; Metarhizium anisopliae; Solanum nigrum; Vitis; apical meristems; breeding; breeding programs; chitinase; coat proteins; cultivars; disease course; disease resistance; epigenetics; fungi; genes; pathogenesis; pathogens; somatic embryos; transfer DNA
Abstract:
... Increased tolerance to pathogens is an important goal in conventional and biotechnology-assisted grapevine breeding programs worldwide. Fungal and viral pathogens cause direct losses in berry production, but also affect the quality of the final products. Precision breeding strategies allow the introduction of resistance characters in elite cultivars, although the factors determining the plant’s ov ...
DOI:
10.1007/s11248-018-0082-1

3. Expression of endogenous proteins in maize hybrids in a multi-location field trial in India

Author:
Gutha, Linga R.; Purushottam, Divakar; Veeramachaneni, Aruna; Tigulla, Sarita; Kodappully, Vikas; Enjala, Chandana; Rajput, Hitendrasinh; Anderson, Jennifer; Hong, Bonnie; Schmidt, Jean; Bagga, Shveta
Source:
Transgenic research 2018 v.27 no.4 pp. 331-342
ISSN:
0962-8819
Subject:
actin; corn; crops; developmental stages; enzyme-linked immunosorbent assay; field experimentation; genotype; glyceraldehyde-3-phosphate dehydrogenase; growing season; hybrids; leaves; models; peptide elongation factors; plant tissues; safety assessment; transgenic plants; India
Abstract:
... Genetically modified (GM) crops undergo large scale multi-location field trials to characterize agronomics, composition, and the concentration of newly expressed protein(s) [herein referred to as transgenic protein(s)]. The concentration of transgenic proteins in different plant tissues and across the developmental stages of the plant is considered in the safety assessment of GM crops. Reference o ...
DOI:
10.1007/s11248-018-0077-y

4. OXI1 kinase plays a key role in resistance of Arabidopsis towards aphids (Myzus persicae)

Author:
Shoala, Tahsin; Edwards, Martin G.; Knight, Marc R.; Gatehouse, Angharad M. R.
Source:
Transgenic research 2018 v.27 no.4 pp. 355-366
ISSN:
0962-8819
Subject:
1,3-beta-glucan synthase; Arabidopsis; Myzus persicae; adulthood; beta-glucanase; biotic stress; breeding programs; callose; coevolution; defense mechanisms; genes; host plants; mitogen-activated protein kinase; mutants; pathogens; phytophagous insects; signal transduction
Abstract:
... Plants have co-evolved with a diverse array of pathogens and insect herbivores and so have evolved an extensive repertoire of constitutive and induced defence mechanisms activated through complex signalling pathways. OXI1 kinase is required for activation of mitogen-activated protein kinases (MAPKs) and is an essential part of the signal transduction pathway linking oxidative burst signals to dive ...
DOI:
10.1007/s11248-018-0078-x

5. Production of functional human CuZn-SOD and EC-SOD in bitransgenic cloned goat milk

Author:
Lu, Rui; Zhang, Ting; Wu, Daijin; He, Zhengyi; Jiang, Lei; Zhou, Minya; Cheng, Yong
Source:
Transgenic research 2018 v.27 no.4 pp. 343-354
ISSN:
0962-8819
Subject:
Southern blotting; Western blotting; beta-casein; bioactive properties; copper; enzyme-linked immunosorbent assay; fibroblasts; food preservation; genetic vectors; goat milk; goats; humans; mammary glands; medical treatment; milk; oxidative stress; photoaging; polymerase chain reaction; reactive oxygen species; regulatory sequences; somatic cells; superoxide dismutase; transgenes; transgenic animals; zinc
Abstract:
... Human copper/zinc superoxide dismutase (CuZn-SOD) and extracellular superoxide dismutase (EC-SOD) are two superoxide dismutases that scavenge reactive oxygen species (ROS). Their biological role of eliminating oxidative stress caused by excessive ROS levels in living organisms has been utilized in medical treatment, preventing skin photoaging and food preservation. In this study, we employed two s ...
DOI:
10.1007/s11248-018-0080-3

6. The assessment of field trials in GMO research around the world and their possible integration in field trials for variety registration

Author:
Slot, M. M.; van de Wiel, C. C. M.; Kleter, G. A.; Visser, R. G. F.; Kok, E. J.
Source:
Transgenic research 2018 v.27 no.4 pp. 321-329
ISSN:
0962-8819
Subject:
cultivars; databases; field experimentation; glycoalkaloids; phenotype; potatoes; protocols; risk assessment; transgenic plants; Netherlands; Sweden
Abstract:
... Most regulations worldwide stipulate that a new genetically modified (GM) crop event has to be compared to its closest non-GM counterpart as a corner stone of the pre-market risk assessment. To this end the GM crop and its comparator should be grown in field trials for a phenotypic comparison as well as for subsequent detailed analysis of the composition of the two crop varieties. A more in-depth ...
DOI:
10.1007/s11248-018-0076-z

7. Unexpected mutations were expected and unrelated to CRISPR-Cas9 activity

Author:
Montoliu, Lluis; Whitelaw, C. Bruce A.
Source:
Transgenic research 2018 v.27 no.4 pp. 315-319
ISSN:
0962-8819
Subject:
genetic drift; genetic polymorphism; genome; mice; sequence analysis; social networks; spontaneous mutation
Abstract:
... The scientific journal Nature Methods have just retracted a publication that reported numerous unexpected mutations after a CRISPR-Cas9 experiment based on collecting whole genome sequencing information from one control and two experimental genome edited mice. In the intervening 10 months since publication the data presented have been strongly contested and criticized by the scientific and biotech ...
DOI:
10.1007/s11248-018-0081-2