PubAg

Main content area

You searched for: Journal Transgenic research Remove constraint Journal: Transgenic research Publication Year 2009 Remove constraint Publication Year: 2009 Source 2009 v.18 no.1 Remove constraint Source: 2009 v.18 no.1 Subject embryonic stem cells Remove constraint Subject: embryonic stem cells
Start Over

Search

2  Search Results

1 - 2 of 2
Number of results to display per page

Search Results

1. Impairment of germline transmission after blastocyst injection with murine embryonic stem cells cultured with mouse hepatitis virus and mouse minute virus

Author:
Mahabir, E.; Reindl, K.; Mysliwietz, J.; Needham, J.; Bulian, D.; Markoullis, K.; Scherb, H.; Schmidt, J.
Source:
Transgenic research 2009 v.18 no.1 pp. 45-57
ISSN:
0962-8819
Subject:
germ cells; Hepatovirus A; blastocyst; mice; cultured cells; viruses; embryonic stem cells; pups
Abstract:
... The aim of this study was to determine the susceptibility of murine embryonic stem (mESCs) to mouse hepatitis virus (MHV-A59) and mouse minute virus (MMVp) and the effect of these viruses on germline transmission (GLT) and the serological status of recipients and pups. When recipients received 10 blastocysts, each injected with 10⁰ TCID₅₀ MHV-A59, three out of five recipients and four out of 14 pu ...
DOI:
10.1007/s11248-008-9216-1
PubMed:
18800235
PubMed Central:
PMC2758372

2. Mycoplasma contamination of murine embryonic stem cells affects cell parameters, germline transmission and chimeric progeny

Author:
Markoullis, Kyriaki; Bulian, Diana; Hölzlwimmer, Gabriele; Quintanilla-Martinez, Leticia; Heiliger, Katrin-Janine; Zitzelsberger, Horst; Scherb, Hagen; Mysliwietz, Josef; Uphoff, Cord C.; Drexler, Hans G.; Adler, Thure; Busch, Dirk H.; Schmidt, Jörg; Mahabir, Esther
Source:
Transgenic research 2009 v.18 no.1 pp. 71-87
ISSN:
0962-8819
Subject:
Mycoplasma; T-lymphocytes; antibodies; blastocyst; cachexia; chimerism; embryo transfer; embryonic stem cells; flow cytometry; germ cells; immunoglobulin M; mice; nose; postnatal development; progeny; pups; viability
Abstract:
... Murine embryonic stem cells (mESCs) inoculated at passage P13 with the mycoplasma species M. hominis, M. fermentans and M. orale and cultured over 20 passages showed reduced growth rate and viability (P < 0.0001) compared to control mESCs. Spectral karyotypic analysis of mycoplasma-infected mESCs showed a number of non-clonal chromosomal aberrations which increased with the duration of infection. ...
DOI:
10.1007/s11248-008-9218-z