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1. Mycoplasma contamination of murine embryonic stem cells affects cell parameters, germline transmission and chimeric progeny

Author:
Markoullis, Kyriaki; Bulian, Diana; Hölzlwimmer, Gabriele; Quintanilla-Martinez, Leticia; Heiliger, Katrin-Janine; Zitzelsberger, Horst; Scherb, Hagen; Mysliwietz, Josef; Uphoff, Cord C.; Drexler, Hans G.; Adler, Thure; Busch, Dirk H.; Schmidt, Jörg; Mahabir, Esther
Source:
Transgenic research 2009 v.18 no.1 pp. 71-87
ISSN:
0962-8819
Subject:
Mycoplasma; T-lymphocytes; antibodies; blastocyst; cachexia; chimerism; embryo transfer; embryonic stem cells; flow cytometry; germ cells; immunoglobulin M; mice; nose; postnatal development; progeny; pups; viability
Abstract:
... Murine embryonic stem cells (mESCs) inoculated at passage P13 with the mycoplasma species M. hominis, M. fermentans and M. orale and cultured over 20 passages showed reduced growth rate and viability (P < 0.0001) compared to control mESCs. Spectral karyotypic analysis of mycoplasma-infected mESCs showed a number of non-clonal chromosomal aberrations which increased with the duration of infection. ...
DOI:
10.1007/s11248-008-9218-z