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You searched for: Journal name Transgenic research Remove constraint Journal name: Transgenic research Subject genotype Remove constraint Subject: genotype
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1. Agronomic performance and transcriptional analysis of carotenoid biosynthesis in fruits of transgenic HighCaro and control tomato lines under field conditions

Author:
Giorio, Giovanni; Stigliani, Adriana Lucia; D'Ambrosio, Caterina
Source:
Transgenic research 2007 v.16 no.1 pp. 15-28
ISSN:
0962-8819
Subject:
Solanum lycopersicum; agronomic traits; beta-carotene; biosynthesis; biotechnology; chromoplasts; developmental stages; field experimentation; fruit quality; fruits; gene expression; genetic engineering; genotype; lycopene; lycopene beta-cyclase; promoter regions; quantitative polymerase chain reaction; reverse transcriptase polymerase chain reaction; statistical analysis; tomatoes; transcription (genetics); transgenes; transgenic plants; Italy
Abstract:
... Genetic manipulation of carotenoid biosynthesis in higher plants has been the objective of a number of biotechnology programs, e.g. the Golden Rice Program. However, tomato (Solanum lycopersicum L.), which naturally accumulates lycopene in fruits, has attracted the attention of many groups who have manipulated it to increase or diversify carotenoid accumulation. One of the most significant achieve ...
DOI:
10.1007/s11248-006-9025-3
PubMed:
17096211
PubMed Central:
PMC2734263

2. Assessing the potential for unintended effects in genetically modified potatoes perturbed in metabolic and developmental processes. Targeted analysis of key nutrients and anti-nutrients

Author:
Louise V. T. Shepherd; James W. McNicol; Ruth Razzo; Mark A. Taylor; Howard V. Davies
Source:
Transgenic research 2006 v.15 no.4 pp. 409-425
ISSN:
0962-8819
Subject:
analysis of variance; antinutritional factors; ascorbic acid; biosynthesis; callus; carbohydrate metabolism; cultivars; fatty acids; gas chromatography-mass spectrometry; genes; genetic engineering; genotype; glycoalkaloids; glycoproteins; kanamycin; metabolites; nitrogen content; nutrients; polyamines; potatoes; principal component analysis; somaclonal variation; tissue culture; toxic substances; transgenic plants; trypsin inhibitors; tubers
Abstract:
... Targeted compositional analysis was carried out on transgenic potato tubers of either cultivar (cv.) Record or cv. Desirée to assess the potential for unintended effects caused by the genetic modification process. The range of transgenic lines analysed included those modified in primary carbohydrate metabolism, polyamine biosynthesis and glycoprotein processing. Controls included wildtype tubers, ...
DOI:
10.1007/s11248-006-0012-5

3. Blastocyst genotyping for quality control of mouse mutant archives: an ethical and economical approach

Author:
Ferdinando Scavizzi; Edward Ryder; Stuart Newman; Marcello Raspa; Diane Gleeson; Hannah Wardle-Jones; Lluis Montoliu; Almudena Fernandez; Marie-Laure Dessain; Vanessa Larrigaldie; Zuzana Khorshidi; Reetta Vuolteenaho; Raija Soininen; Philippe André; Sylvie Jacquot; Yi Hong; Martin Hrabe de Angelis; Ramiro Ramirez-Solis; Brendan Doe
Source:
Transgenic research 2015 v.24 no.5 pp. 921-927
ISSN:
0962-8819
Subject:
blastocyst; cages; cryopreservation; economic resources; ethics; genes; genetically modified organisms; genotype; genotyping; knockout mutants; mice; mutagenesis; phenotype; progeny; pups; quality control; spermatozoa
Abstract:
... With the advent of modern developmental biology and molecular genetics, the scientific community has generated thousands of newly genetically altered strains of laboratory mice with the aim of elucidating gene function. To this end, a large group of Institutions which form the International Mouse Phenotyping Consortium is generating and phenotyping a knockout mouse strain for each of the ~20,000 p ...
DOI:
10.1007/s11248-015-9897-1
PubMed:
26178246
PubMed Central:
PMC4569667

4. The Chitinase A from the baculovirus AcMNPV enhances resistance to both fungi and herbivorous pests in tobacco

Author:
Corrado, Giandomenico; Arciello, Stefania; Fanti, Paolo; Fiandra, Luisa; Garonna, Antonio; Digilio, Maria Cristina; Lorito, Matteo; Giordana, Barbara; Pennacchio, Francesco; Rao, Rosa
Source:
Transgenic research 2008 v.17 no.4 pp. 557-571
ISSN:
0962-8819
Subject:
Aphidoidea; Autographa californica multiple nucleopolyhedrovirus; Lepidoptera; biotechnology; biotic stress; breeding; chitinase; fungi; genes; genotype; herbivores; insecticidal properties; insects; larvae; pathogens; pests; tobacco; transgenic plants
Abstract:
... Biotechnology has allowed the development of novel strategies to obtain plants that are more resistant to pests, fungal pathogens and other agents of biotic stress. The obvious advantages of having genotypes with multiple beneficial traits have recently fostered the development of gene pyramiding strategies, but less attention has been given to the study of genes that can increase resistance to di ...
DOI:
10.1007/s11248-007-9129-4
PubMed:
17851776

5. Comparison of Agrobacterium and particle bombardment using whole plasmid or minimal cassette for production of high-expressing, low-copy transgenic plants

Author:
Mark A. Jackson; David J. Anderson; Robert G. Birch
Source:
Transgenic research 2013 v.22 no.1 pp. 143-151
ISSN:
0962-8819
Subject:
Agrobacterium; gene expression; gene transfer; genotype; plasmids; quantitative polymerase chain reaction; reporter genes; sugarcane; transcription (genetics); transgenes; transgenic plants
Abstract:
... Transgene integration complexity in the recipient genome can be an important determinant of transgene expression and field performance in transgenic crops. We provide the first direct comparison of Agrobacterium-mediated transformation (AMT) and particle bombardment using whole plasmid (WP) and excised minimal cassettes (MC), for transformation efficiency, transgene integration complexity and tran ...
DOI:
10.1007/s11248-012-9639-6
PubMed:
22869288

6. Conditional Allele Mouse Planner (CAMP): software to facilitate the planning and design of breeding strategies involving mice with conditional alleles

Author:
Jason D. Hoffert; Trairak Pisitkun; R. Lance Miller
Source:
Transgenic research 2012 v.21 no.3 pp. 665-669
ISSN:
0962-8819
Subject:
alleles; animal models; breeding; computer software; genotype; litter size; mice; planning
Abstract:
... Transgenic and conditional knockout mouse models play an important role in biomedical research and their use has grown exponentially in the last 5–10 years. Generating conditional knockouts often requires breeding multiple alleles onto the background of a single mouse or group of mice. Breeding these mice depends on parental genotype, litter size, transmission frequency, and the number of breeding ...
DOI:
10.1007/s11248-011-9545-3
PubMed:
21870117

7. Development of a facile genetic transformation system for the Spanish elite rice paella genotype Bomba

Author:
Andrea Saba-Mayoral; Ludovic Bassie; Paul Christou; Teresa Capell
Source:
Transgenic research 2022 v.31 no.3 pp. 325-340
ISSN:
0962-8819
Subject:
Mendelian inheritance; callus; genetic engineering; genetic transformation; genetically modified organisms; genotype; gold; plasmids; rice; transgenes
Abstract:
... We report the development of an efficient and reproducible genetic transformation system for the recalcitrant Spanish elite rice paella genotype, Bomba. Preconditioned embryos derived from dry seeds were bombarded with gold particles carrying a plasmid containing a screenable and a selectable marker. We confirmed integration and expression of hpt and gusA in the rice genome. Transformation frequen ...
DOI:
10.1007/s11248-022-00303-z

8. Development of an in planta method for transformation of alfalfa (Medicago sativa)

Author:
Weeks, J Troy; Ye, Jingsong; Rommens, Caius M
Source:
Transgenic research 2008 v.17 no.4 pp. 587-597
ISSN:
0962-8819
Subject:
Agrobacterium; Medicago sativa; alfalfa; caffeate O-methyltransferase; forage quality; genes; genetic markers; genetic transformation; genetically modified organisms; genotype; lignin; sand; seedlings; tissue culture; transfer DNA
Abstract:
... Conventional methods in transforming alfalfa (Medicago sativa) require multiple tissue culture manipulations that are time-consuming and expensive, while applicable only to a few highly regenerable genotypes. Here, we describe a simple in planta method that makes it possible to transform a commercial variety without employing selectable marker genes. Basically, young seedlings are cut at the apica ...
DOI:
10.1007/s11248-007-9132-9
PubMed:
17851774

9. Discoidin domain receptor 2 (DDR2) regulates body size and fat metabolism in mice

Author:
Ikuma Kawai; Hirokazu Matsumura; Wataru Fujii; Kunihiko Naito; Ken Kusakabe; Yasuo Kiso; Kiyoshi Kano
Source:
Transgenic research 2014 v.23 no.1 pp. 165-175
ISSN:
0962-8819
Subject:
body length; body weight; cell adhesion; cell proliferation; dwarfing; extracellular matrix; gene overexpression; genotype; hormones; lipid metabolism; mice; null alleles; screening
Abstract:
... Discoidin domain receptor 2 (DDR2) is a receptor tyrosine kinase that is activated by fibrillar collagens, which act as its endogenous ligand. DDR2 regulates cell proliferation, cell adhesion, migration, extracellular matrix remodeling and reproductive functions. Both DDR2 null allele mice and mice with a recessive, loss-of-function allele for Ddr2 exhibit dwarfing and a reduction in body weight. ...
DOI:
10.1007/s11248-013-9751-2
PubMed:
24036888

10. Evaluation of endophyte toxin production and its interaction with transgenic perennial ryegrass (Lolium perenne L.) with altered expression of fructosyltransferases

Author:
Paula Andrea Giraldo; Carly Elliott; Pieter Badenhorst; Gavin Kearney; German C. Spangenberg; Noel O. I. Cogan; Kevin F. Smith
Source:
Transgenic research 2018 v.27 no.5 pp. 397-407
ISSN:
0962-8819
Subject:
Lolium perenne; carbohydrates; cultivars; endophytes; ergovaline; forage; fungal biomass; genetic engineering; genetically modified organisms; genotype; host plants; risk; toxicity; toxins; transgenes; water solubility
Abstract:
... Alkaloid concentration of perennial ryegrass herbage is affected by endophyte strain and host plant genotype. However, previous studies suggest that associations between host and endophyte also depends on environmental conditions, especially those affecting nutrient reserves and that water-soluble carbohydrate (WSC) concentration of perennial ryegrass plants may influence grass-endophyte associati ...
DOI:
10.1007/s11248-018-0087-9

11. Evaluation of p53 genotype on gene expression in the testis, liver, and heart from male C57BL/6 mice

Author:
Dayton M. Petibone; Rohan M. Kulkarni; Ching-Wei Chang; James J. Chen; Suzanne M. Morris
Source:
Transgenic research 2012 v.21 no.2 pp. 257-263
ISSN:
0962-8819
Subject:
quantitative polymerase chain reaction; liver; cell division; gene expression; mice; animal models; DNA repair; genes; genotype; multivariate analysis; testes; heart
Abstract:
... Our laboratory is conducting experiments designed to characterize the role of p53 in gene expression in the TSG-p53® mouse model. In the study reported here, gene expression levels in tissue derived from the testis, liver, and heart of male, 8–9 week old, p53 wild-type (WT), heterozygous (HET) or knockout (KO) mice were determined utilizing a targeted qPCR 84-gene array. The heart, liver and testi ...
DOI:
10.1007/s11248-011-9526-6
PubMed:
21656205

12. Expression of endogenous proteins in maize hybrids in a multi-location field trial in India

Author:
Linga R. Gutha; Divakar Purushottam; Aruna Veeramachaneni; Sarita Tigulla; Vikas Kodappully; Chandana Enjala; Hitendrasinh Rajput; Jennifer Anderson; Bonnie Hong; Jean Schmidt; Shveta Bagga
Source:
Transgenic research 2018 v.27 no.4 pp. 331-342
ISSN:
0962-8819
Subject:
actin; corn; crops; developmental stages; enzyme-linked immunosorbent assay; field experimentation; genotype; glyceraldehyde-3-phosphate dehydrogenase; growing season; hybrids; leaves; models; peptide elongation factors; plant tissues; safety assessment; transgenic plants; India
Abstract:
... Genetically modified (GM) crops undergo large scale multi-location field trials to characterize agronomics, composition, and the concentration of newly expressed protein(s) [herein referred to as transgenic protein(s)]. The concentration of transgenic proteins in different plant tissues and across the developmental stages of the plant is considered in the safety assessment of GM crops. Reference o ...
DOI:
10.1007/s11248-018-0077-y

13. Expression of the murine wild-type tyrosinase gene in transgenic rabbits

Author:
Aigner, B.; Besenfelder, U.; Seregi, J.; Frenyo, L.V.; Sahin-Toth, T.; Brem, G.
Source:
Transgenic research 1996 v.5 no.6 pp. 405-411
ISSN:
0962-8819
Subject:
rabbits; transgenic animals; gene transfer; mice; monophenol monooxygenase; structural genes; gene expression; pigmentation; genetic markers; animal breeding; inheritance (genetics); phenotype; genotype
DOI:
10.1007/BF01980205
PubMed:
8840523

14. Functional ability of cytoskeletal β-actin regulator to drive constitutive and ubiquitous expression of a fluorescent reporter throughout the life cycle of transgenic marine medaka Oryzias dancena

Author:
Young Sun Cho; Sang Yoon Lee; Youn Kyoung Kim; Dong Soo Kim; Yoon Kwon Nam
Source:
Transgenic research 2011 v.20 no.6 pp. 1333-1355
ISSN:
0962-8819
Subject:
Oryzias; actin; adults; bioinformatics; color; cytoskeleton; fluorescence; gene expression; genetically modified organisms; genotype; longevity; progeny; transcription factors; transgenes
Abstract:
... Marine medaka Oryzias dancena, a candidate model organism, represents many attractive merits as a material for experimental transgenesis and/or heterologous expression assay particularly in the field of ecotoxicology and developmental biology. In this study, cytoskeletal β-actin gene was characterized from O. dancena and the functional capability of its promoter to drive constitutive expression of ...
DOI:
10.1007/s11248-011-9501-2
PubMed:
21437716

15. Generation and characterization of a GFP transgenic rat line for embryological research

Author:
Elena Popova; Brit Rentzsch; Michael Bader; Alexander Krivokharchenko
Source:
Transgenic research 2008 v.17 no.5 pp. 955-963
ISSN:
0962-8819
Subject:
green fluorescent protein; superovulation; fluorescence; developmental stages; parents; early development; models; blastocyst; gene expression; genes; genotype; progeny; embryo transfer; ovulation; females; rats
Abstract:
... Model organisms expressing fluorescent proteins are important tools for research. The present study was performed to generate and characterize a new line of green fluorescent protein (GFP) transgenic rats for use as a model in experimental embryological research. We injected a GFP expression vector into 135 zygotes of the Sprague-Dawley (SD) rat strain. Embryo transfer of 103 surviving embryos res ...
DOI:
10.1007/s11248-008-9189-0
PubMed:
18523856

16. Generation of insulin-deficient piglets by disrupting INS gene using CRISPR/Cas9 system

Author:
Bumrae Cho; Su Jin Kim; Eun-Jin Lee; Sun Mi Ahn; Jin Seok Lee; Dal-Young Ji; Kiho Lee; Jung-Taek Kang
Source:
Transgenic research 2018 v.27 no.3 pp. 289-300
ISSN:
0962-8819
Subject:
animal models; blood glucose; blood serum; c-peptide; chronic diseases; diabetes mellitus; embryo transfer; genes; genetic engineering; genotype; glucose; humans; metabolism; new drugs; pancreas; pathogenesis; pathophysiology; piglets; rodents; somatic cells; urine
Abstract:
... Diabetes mellitus is a chronic disease with accompanying severe complications. Various animal models, mostly rodents due to availability of genetically modified lines, have been used to investigate the pathophysiology of diabetes. Using pigs for diabetic research can be beneficial because of their similarity in size, pathogenesis pathway, physiology, and metabolism with human. However, the use of ...
DOI:
10.1007/s11248-018-0074-1

17. Genetic polymorphisms among C57BL/6 mouse inbred strains

Author:
Zurita, Esther; Chagoyen, Mónica; Cantero, Marta; Alonso, Rosario; González-Neira, Anna; López-Jiménez, Alejandro; López-Moreno, José Antonio; Landel, Carlisle P.; Benítez, Javier; Pazos, Florencio; Montoliu, Lluís
Source:
Transgenic research 2011 v.20 no.3 pp. 481-489
ISSN:
0962-8819
Subject:
animal models; chromosome mapping; genetic drift; genetic variation; genotype; mice; monitoring; single nucleotide polymorphism
Abstract:
... Mice from the inbred C57BL/6 strain have been commonly used for the generation and analysis of transgenic and knockout animal models. However, several C57BL/6 substrains exist, and these are genetically and phenotypically different. In addition, each of these substrains can be purchased from different animal providers and, in some cases, they have maintained their breeding stocks separated for a l ...
DOI:
10.1007/s11248-010-9403-8
PubMed:
20506040

18. Goat RSPO1 over-expression rescues sex-reversal in Rspo1-knockout XX mice but does not perturb testis differentiation in XY or sex-reversed XX mice

Author:
Buscara, Laurine; Montazer-Torbati, Fatemeh; Chadi, Sead; Auguste, Aurélie; Laubier, Johann; Chassot, Anne-Amandine; Renault, Lauriane; Passet, Bruno; Costa, José; Pannetier, Maëlle; Vilotte, Marthe; Chaboissier, Marie-Christine; Vilotte, Jean-Luc; Pailhoux, Eric; Le Provost, Fabienne
Source:
Transgenic research 2009 v.18 no.4 pp. 649-654
ISSN:
0962-8819
Subject:
bacterial artificial chromosomes; clones; females; gene overexpression; genotype; goats; introgression; mice; oocytes; phenotype; sexual development; testes; transgenes
Abstract:
... RSPO1 is a newly discovered gene involved in sex differentiation. Two goat BAC clones encompassing the RSPO1 gene (gRSPO1) were injected into mouse oocytes and several transgenic lines derived. Both clones induced gRSPO1 over-expression in various tissues, including male and female gonads, with no obvious phenotype and normal sex-ratios. Introgression of the gRSPO1 transgene into a mouse RSPO1 kno ...
DOI:
10.1007/s11248-009-9247-2
PubMed:
19184501

19. Impact of long-term cropping of glyphosate-resistant transgenic soybean [Glycine max (L.) Merr.] on soil microbiome

Author:
Letícia Carlos Babujia; Adriana Pereira Silva; André Shigueyoshi Nakatani; Mauricio Egidio Cantão; Ana Tereza Ribeiro Vasconcelos; Jesuí Vergilio Visentainer; Mariangela Hungria
Source:
Transgenic research 2016 v.25 no.4 pp. 425-440
ISSN:
0962-8819
Subject:
Acidobacteria; Actinomycetales; Burkholderiales; Chlorophyta; Glycine max; Myxococcales; Pseudomonadales; Rhizobiales; amino acids; buffering capacity; carbohydrates; cell division; field experimentation; genetic engineering; genotype; glyphosate; grain yield; herbicide resistance; long term effects; metagenomics; microbial biomass; microbiological quality; microbiome; physical properties; protein metabolism; soil; soil fertility; soil microorganisms; soybeans; transgenes; transgenic plants; Brazil
Abstract:
... The transgenic soybean [Glycine max (L.) Merrill] occupies about 80 % of the global area cropped with this legume, the majority comprising the glyphosate-resistant trait (Roundup Ready®, GR or RR). However, concerns about possible impacts of transgenic crops on soil microbial communities are often raised. We investigated soil chemical, physical and microbiological properties, and grain yields in l ...
DOI:
10.1007/s11248-016-9938-4

20. Impacts on the metabolome of down-regulating polyphenol oxidase in potato tubers

Author:
Louise Vida Traill Shepherd; Colin James Alexander; Christine Anne Hackett; Diane McRae; Julia Anne Sungurtas; Susan Ramsay Verrall; Jennifer Anne Morris; Peter Edward Hedley; David Rockhold; William Belknap; Howard Vivian Davies
Source:
Transgenic research 2015 v.24 no.3 pp. 447-461
ISSN:
0962-8819
Subject:
Solanum tuberosum; analysis of variance; catechol oxidase; discoloration; enzyme activity; gas chromatography; gene expression; genetically modified organisms; genotype; liquid chromatography; mass spectrometry; mechanical damage; metabolites; metabolome; potatoes; principal component analysis; slicing; temporal variation; tubers
Abstract:
... Tubers of potato (Solanum tuberosum L. cv. Estima) genetically modified to reduce polyphenol oxidase (PPO) activity and enzymatic discolouration were assessed for changes in the metabolome using Liquid Chromatography–Mass Spectrometry (LC–MS) and Gas Chromatography (GC)–MS. Metabolome changes induced over a 48 hour (h) period by tuber wounding (sliced transverse sections) were also assessed using ...
DOI:
10.1007/s11248-014-9850-8

21. Low crop plant population densities promote pollen-mediated gene flow in spring wheat (Triticum aestivum L.)

Author:
Willenborg, Christian J.; Brûlé-Babel, Anita L.; Van Acker, Rene C.
Source:
Transgenic research 2009 v.18 no.6 pp. 841-854
ISSN:
0962-8819
Subject:
Triticum aestivum; best management practices; crops; cultivars; field experimentation; flowering; gene flow; genotype; hybridization; mechanistic models; plant density; spring wheat; transgenes; yield components
Abstract:
... Transgenic wheat is currently being field tested with the intent of eventual commercialization. The development of wheat genotypes with novel traits has raised concerns regarding the presence of volunteer wheat populations and the role they may play in facilitating transgene movement. Here, we report the results of a field experiment that investigated the potential of spring wheat plant population ...
DOI:
10.1007/s11248-009-9267-y
PubMed:
19387859

22. Mendel: a simple excel workbook to compare the observed and expected distributions of genotypes/phenotypes in transgenic and knockout mouse crosses involving up to three unlinked loci by means of a χ2 test

Author:
Lluís Montoliu
Source:
Transgenic research 2012 v.21 no.3 pp. 677-681
ISSN:
0962-8819
Subject:
crossing; genotype; loci; mice; phenotype
Abstract:
... The analysis of transgenic and knockout mice always involves the establishment of matings with individuals carrying different loci, segregating independently, whose presence is expected among the progeny, according to a Mendelian distribution. The appearance of distorted inheritance ratios suggests the existence of unexpected lethal or sub-lethal phenotypes associated with some genotypes. These si ...
DOI:
10.1007/s11248-011-9544-4
PubMed:
21853295

23. Over-expression of the MxIRT1 gene increases iron and zinc content in rice seeds

Author:
Song Tan; Rui Han; Peng Li; Guang Yang; Shuang Li; Peng Zhang; Wei-Bing Wang; Wei-Zhong Zhao; Li-Ping Yin
Source:
Transgenic research 2015 v.24 no.1 pp. 109-122
ISSN:
0962-8819
Subject:
Malus; absorption; apples; biofortification; cadmium; gene overexpression; genes; genotype; iron; phenotype; protoplasts; rice; seeds; transgenic plants; trees; zinc
Abstract:
... Iron and zinc are essential in plant and human nutrition. Iron deficiency has been one of the causes of human mortality, especially in developing countries with high rice consumption. MxIRT1 is a ferrous transporter that has been screened from an iron-efficient genotype of the apple tree, Malus xiaojinensis Cheng et Jiang. In order to produce Fe-biofortified rice with MxIRT1 to solve the Fe-defici ...
DOI:
10.1007/s11248-014-9822-z
PubMed:
25099285

24. Production of Phytophthora infestans-resistant potato (Solanum tuberosum) utilising Ensifer adhaerens OV14

Author:
Toni Wendt; Fiona Doohan; Ewen Mullins
Source:
Transgenic research 2012 v.21 no.3 pp. 567-578
ISSN:
0962-8819
Subject:
Arabidopsis thaliana; genetic transformation; gene transfer; rhizosphere; phenotype; Solanum tuberosum; Phytophthora infestans; Ensifer adhaerens; potatoes; genes; genetic engineering; genotype; leaves; Solanum bulbocastanum; Agrobacterium; bacteria
Abstract:
... Based on the use of Agrobacterium tumefaciens-mediated transformation commodity crop improvement through genetic engineering is the fastest adopted crop technology in the world (James 2010). However, the complexity of the Agrobacterium patent landscape remains a challenge for non-patent holders who wish to generate novel varieties for a commercial purpose. The potential of non-Agrobacterium strain ...
DOI:
10.1007/s11248-011-9553-3
PubMed:
21912851

25. Production of Prnp ⁻/⁻ goats by gene targeting in adult fibroblasts

Author:
Zhu, Caihong; Li, Bei; Yu, Guohua; Chen, Jianquan; Yu, Huiqing; Chen, Juan; Xu, Xujun; Wu, Youbing; Zhang, Aimin; Cheng, Guoxiang
Source:
Transgenic research 2009 v.18 no.2 pp. 163-171
ISSN:
0962-8819
Subject:
adults; alleles; cloning (cells); fetus; fibroblasts; gene targeting; genotype; goats; mice; pregnancy; scrapie; somatic cells
Abstract:
... Homozygous mice devoid of functional Prnp are resistant to scrapie and prion propagation, but heterozygous mice for Prnp disruption still suffer from prion disease and prion deposition. We have previously generated heterozygous cloned goats with one allele of Prnp functional disruption. To obtain goats with both alleles of Prnp be disrupted which would be resistant to scrapie completely, a second- ...
DOI:
10.1007/s11248-008-9220-5

26. Production of genetically modified pigs expressing human insulin and C-peptide as a source of islets for xenotransplantation

Author:
Bumrae Cho; Eun-Jin Lee; Sun Mi Ahn; Ghangyong Kim; Sang Hoon Lee; Dal-Young Ji; Jung-Taek Kang
Source:
Transgenic research 2019 v.28 no.5-6 pp. 549-559
ISSN:
0962-8819
Subject:
amino acids; blood glucose; blood serum; c-peptide; fibroblasts; genes; genetic engineering; genotype; glucose; humans; liquid chromatography; pancreas; phenotype; somatic cells; tandem mass spectrometry; xenotransplantation
Abstract:
... Islet xenotransplantation is a promising treatment for type I diabetes. Numerous studies of islet xenotransplantation have used pig-to-nonhuman primate transplantation models. Some studies reported long-term survival and successful function of porcine islets in diabetic monkeys. Genetic engineering techniques may improve the survival and function of porcine islets. A recent study reported the gene ...
DOI:
10.1007/s11248-019-00169-8

27. Proteome rebalancing in transgenic Camelina occurs within the enlarged proteome induced by β-carotene accumulation and storage protein suppression

Author:
Monica A. Schmidt; Ken Pendarvis
Source:
Transgenic research 2017 v.26 no.2 pp. 171-186
ISSN:
0962-8819
Subject:
Camelina sativa; GRAS substances; RNA interference; albumins; antibiotic resistance; beta-carotene; carbohydrates; genes; genetic engineering; genetically modified organisms; genotype; heterologous gene expression; industrial crops; lipid content; metabolome; oils; oilseed crops; oilseeds; phytoene synthase; protein content; proteome; seeds
Abstract:
... Oilseed crops are global commodities for their oil and protein seed content. We have engineered the oilseed Camelina sativa to exhibit increased protein content with a slight decrease in oil content. The introduction of a phytoene synthase gene with an RNAi cassette directed to suppress the storage protein 2S albumin resulted in seeds with an 11–24 % elevation in overall protein. The phytoene synt ...
DOI:
10.1007/s11248-016-9992-y

28. Rapid and reliable determination of transgene zygosity in mice by multiplex ligation-dependent probe amplification

Author:
Notini, Amanda J.; Li, Ruili; Western, Patrick S.; Sinclair, Andrew H.; White, Stefan J.
Source:
Transgenic research 2009 v.18 no.6 pp. 987-991
ISSN:
0962-8819
Subject:
green fluorescent protein; alleles; loci; transgenes; DNA; polymerase chain reaction; beta-galactosidase; sex determination analysis; mice; tail; genotype; biopsy; Y chromosome; neomycin
Abstract:
... The ability to rapidly and unequivocally distinguish heterozygous from homozygous transgenic mice is an integral part of any breeding strategy. Here we describe a quick and simple protocol for determining the zygosity of transgenic mice at multiple loci in a single reaction. This involved the development of a multiplex ligation-dependent probe amplification (MLPA) probe mix to simultaneously measu ...
DOI:
10.1007/s11248-009-9284-x
PubMed:
19504348

29. Reproductive abnormalities in mice expressing omega-3 fatty acid desaturase in their mammary glands

Author:
William E. Pohlmeier; Russell C. Hovey; Alison L. Van Eenennaam
Source:
Transgenic research 2011 v.20 no.2 pp. 283-292
ISSN:
0962-8819
Subject:
Caenorhabditis elegans; animal ovaries; beta-casein; biosynthesis; embryo transfer; females; fertilizer rates; genetically modified organisms; genotype; goats; juveniles; lactation; litter size; mammary glands; mice; omega-3 fatty acids; omega-6 fatty acids; oocytes; ovulation; phenotype; phospholipids; polyunsaturated fatty acids; prostaglandins; reproductive performance; resorption; stearoyl-CoA desaturase; transcription (genetics); transgenes; uterus
Abstract:
... The Caenorhabditis elegans n-3 fatty acid desaturase (Fat-1) acts on a range of 18- and 20-carbon n-6 fatty acid substrates. Transgenic female mice expressing the Fat-1 gene under transcriptional control of the goat β-casein promoter produce milk phospholipids having elevated levels of n-3 polyunsaturated fatty acids (PUFA). However, females from this line were also observed to have impaired repro ...
DOI:
10.1007/s11248-010-9407-4
PubMed:
20532624
PubMed Central:
PMC3051059

30. Rescue of germline transmission from chimeras by IVF after sperm analysis

Author:
Ming-Wen Li; Brandon J. Willis; Kristin D. Evans; Renee S. Araiza; Angus Yiu-Fai Lee; K. C. Kent Lloyd
Source:
Transgenic research 2015 v.24 no.1 pp. 99-108
ISSN:
0962-8819
Subject:
alleles; blastocyst; chimerism; clones; color; genotype; genotyping; in vitro fertilization; male fertility; mice; morula; mutants; natural mating; progeny; spermatogenesis; spermatozoa
Abstract:
... Successful production of genetically modified mouse lines is dependent on germline transmission (GLT) of mutant alleles from chimeras. When natural mating fails to achieve GLT due to male infertility, sickness, or other problems, sperm can be harvested from chimeras and used for assisted reproductive technologies such as in vitro fertilization (IVF) to attempt to “rescue” GLT. However, a rational, ...
DOI:
10.1007/s11248-014-9819-7
PubMed:
25080098
PubMed Central:
PMC4276497 [Available on 02/01/16]

31. Testing coexistence and genetic containment for an autogamous crop

Author:
Wang, Tianyu; Shi, Yunsu; Li, Yu; Darmency, Henri
Source:
Transgenic research 2009 v.18 no.5 pp. 809-813
ISSN:
0962-8819
Subject:
Setaria italica; crops; cultivars; field experimentation; genes; genotype; herbicide resistance; models; phenotype; pollen flow; rice; risk; seeds; wheat
Abstract:
... Is there any risk that the threshold for admixture of genetically modified seeds in the harvest of a conventional cultivar, 0.9% in Europe, will be exceeded in the case of inbreeder crops? Using herbicide-resistant foxtail millet, Setaria italica, as a model of a preferentially autogamous crop, such as wheat and rice, field experiments show that genotype admixture due to pollen flow between adjace ...
DOI:
10.1007/s11248-009-9270-3
PubMed:
19404764

32. Transgenic accumulation of a defective cucumber mosaic virus (CMV) replicase derived double stranded RNA modulates plant defence against CMV strains O and Y in potato

Author:
Valentine Otang Ntui; Kong Kynet; Pejman Azadi; Raham Sher Khan; Dong Poh Chin; Ikuo Nakamura; Masahiro Mii
Source:
Transgenic research 2013 v.22 no.6 pp. 1191-1205
ISSN:
0962-8819
Subject:
Cucumber mosaic virus; RNA interference; cultivars; genes; genotype; host range; pathogenicity; phenotype; plant pathogens; potatoes; small interfering RNA; transcription (genetics); viruses
Abstract:
... Cucumber mosaic virus is an important plant pathogen with a broad host range encompassing many plant species. This study demonstrates the production of transgenic potato lines exhibiting complete resistance to cucumber mosaic virus strain O and Y by post transcriptional gene silencing. Two constructs were used, one, pEKH2IN2CMVai, contains inverted repeat of 1,138 bp fragment of a defective CMV re ...
DOI:
10.1007/s11248-013-9721-8
PubMed:
23748933

33. Transgenic carnations obtained by Agrobacterium tumefaciens-mediated transformation of leaf explants

Author:
Altvorst, A.C. van; Riksen, T.; Koehorst, H.; Dons, H.J.M.
Source:
Transgenic research 1995 v.4 no.2 pp. 105-113
ISSN:
0962-8819
Subject:
genetic transformation; gene transfer; cultivars; kanamycin; transgenic plants; Agrobacterium radiobacter; explants; phosphotransferases (phosphomutases); genotype; Dianthus caryophyllus; reporter genes; beta-glucuronidase; leaves; drug resistance
DOI:
10.1007/BF01969412

34. Transgenic expression in citrus of single-chain antibody fragments specific to Citrus tristeza virus confers virus resistance

Author:
Cervera, Magdalena; Esteban, Olga; Gil, Maite; Gorris, M. Teresa; Martínez, M. Carmen; Peña, Leandro; Cambra, Mariano
Source:
Transgenic research 2010 v.19 no.6 pp. 1001-1015
ISSN:
0962-8819
Subject:
Citrus; Citrus tristeza virus; coat proteins; epitopes; gene expression; genetic engineering; genotype; monoclonal antibodies; pathogens; protective effect; recombinant antibodies; transgenes; transgenic plants; viruses
Abstract:
... Citrus tristeza virus (CTV) causes one of the most destructive viral diseases of citrus worldwide. Generation of resistant citrus genotypes through genetic engineering could be a good alternative to control CTV. To study whether production of single-chain variable fragment (scFv) antibodies in citrus could interfere and immunomodulate CTV infection, transgenic Mexican lime plants expressing two di ...
DOI:
10.1007/s11248-010-9378-5

35. Zygote injection of CRISPR/Cas9 RNA successfully modifies the target gene without delaying blastocyst development or altering the sex ratio in pigs

Author:
Kristin M. Whitworth; Joshua A. Benne; Lee D. Spate; Stephanie L. Murphy; Melissa S. Samuel; Clifton N. Murphy; Jürgen A. Richt; Eric Walters; Randall S. Prather; Kevin D. Wells
Source:
Transgenic research 2017 v.26 no.1 pp. 97-107
ISSN:
0962-8819
Subject:
gilts; serine; proteinases; DNA; males; gene editing; models; polymerase chain reaction; sex ratio; blastocyst; transcription (genetics); transgenic animals; cytoplasm; genotype; start codon; zygote; RNA; estrus; piglets
Abstract:
... The CRISPR/Cas9 genome editing tool has increased the efficiency of creating genetically modified pigs for use as biomedical or agricultural models. The objectives were to determine if DNA editing resulted in a delay in development to the blastocyst stage or in a skewing of the sex ratio. Six DNA templates (gBlocks) that were designed to express guide RNAs that target the transmembrane protease, s ...
DOI:
10.1007/s11248-016-9989-6
PubMed:
27744533
PubMed Central:
PMC5247313

36. The impact of selection parameters on the phenotype and genotype of transgenic rice callus and plants

Author:
Christou, P.; Ford, T.L.
Source:
Transgenic research 1995 v.4 no.1 pp. 44-51
ISSN:
0962-8819
Subject:
hygromycin B; genetic transformation; phenotype; Oryza sativa; transgenic plants; explants; chimerism; genotype; reporter genes; herbicide resistance; bilanafos; beta-glucuronidase; callus; drug resistance
DOI:
10.1007/BF01976501

37. A model to predict the frequency of integration of fitness-related QTLs from cultivated to wild soybean

Author:
N. Kitamoto; A. Kaga; Y. Kuroda; R. Ohsawa
Source:
Transgenic research 2012 v.21 no.1 pp. 131-138
ISSN:
0962-8819
Subject:
Glycine max; Glycine soja; alleles; domestication; extinction; genotype; introgression; land use; models; outcrossing; overwintering; quantitative trait loci; risk; seeds; selfing; soybeans; transgenes; transgenic plants; winter
Abstract:
... With the proliferation of genetically modified (GM) products and the almost exponential growth of land use for GM crops, there is a growing need to develop quantitative approaches to estimating the risk of escape of transgenes into wild populations of crop relatives by natural hybridization. We assessed the risk of transgene escape by constructing a population genetic model based on information on ...
DOI:
10.1007/s11248-011-9516-8
PubMed:
21544624