U.S. flag

An official website of the United States government

Dot gov

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Https

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PubAg

Main content area

You searched for: Journal name Transgenic research Remove constraint Journal name: Transgenic research Subject term actin Remove constraint Subject term: actin
Start Over

Search

19  Search Results

1 - 19 of 19
Number of results to display per page

Search Results

1. Expression of a 434:VP16 chimeric activator leads to high-level activation of gene expression in stable transformants of Arabidopsis

Author:
Storgaard, M.; Didion, T.; Okkels, F.; Nielsen, K.K.
Source:
Transgenic research 2002 v.11 no.2 pp. 151-159
ISSN:
0962-8819
Subject:
Arabidopsis thaliana; transgenic plants; gene expression; transcription factors; bacteriophages; recombinant proteins; actin; ubiquitin; Zea mays; Oryza sativa; beta-glucuronidase; reporter genes; genetic transformation; promoter regions
PubMed:
12054349

2. Sequences of transgene insertion sites in transgenic F4 common carp

Author:
Wu, B.; Sun, Y.H.; Wang, Y.P.; Wang, Y.W.; Zhu, Z.Y.
Source:
Transgenic research 2004 v.13 no.2 pp. 95-96
ISSN:
0962-8819
Subject:
genetic transformation; gene transfer; hybrids; transgenes; phosphoglycerate kinase; humans; carp; hybridization; transgenic animals; Cyprinus carpio; mice; keratin; actin; promoter regions
DOI:
10.1023/B:TRAG.0000026123.01453.5b
PubMed:
15198197

3. The commonly used β-actin-GFP transgenic mouse strain develops a distinct type of glomerulosclerosis

Author:
Guo, Jian-Kan; Cheng, Ee-Chun; Wang, Lin; Swenson, E. Scott; Ardito, Thomas A.; Kashgarian, Michael; Cantley, Lloyd G.; Krause, Diane S.
Source:
Transgenic research 2007 v.16 no.6 pp. 829-834
ISSN:
0962-8819
Subject:
Western blotting; actin; basement membrane; extracellular matrix; green fluorescent protein; insertional mutagenesis; mice; polymerase chain reaction; transcription (genetics); transgenes; transgenic animals
Abstract:
... Green fluorescent protein (GFP) transgenic animals are widely used in biomedical research. We observed that the commonly used β-actin-GFP transgenic mouse has renal defects with proteinuria starting as early as 5 weeks of age. Histological analysis reveals a widespread increase in glomerular extracellular matrix, occasional mesangiolysis, and secondary tubulointerstitial injury. Electron microscop ...
DOI:
10.1007/s11248-007-9107-x

4. Systemic overexpression of growth hormone (GH) in transgenic FVB/N inbred mice: an optimized model for holistic studies of molecular mechanisms underlying GH-induced kidney pathology

Author:
von Waldthausen, Dagmar C.; Schneider, Marlon R.; Renner-Müller, Ingrid; Rauleder, Dirk N.; Herbach, Nadja; Aigner, Bernhard; Wanke, Rüdiger; Wolf, Eckhard
Source:
Transgenic research 2008 v.17 no.4 pp. 479-488
ISSN:
0962-8819
Subject:
actin; animal models; cattle; chickens; gene overexpression; genetic background; hybrids; kidneys; long term effects; messenger RNA; mice; modifiers (genes); proteome; somatotropin; transcriptome
Abstract:
... Transgenic mice overexpressing growth hormone (GH) display a plethora of phenotypic alterations and provide unique models for studying and influencing consequences of chronic GH excess. Since the first report on GH transgenic mice was published in 1982, many different mouse models overexpressing GH from various species at different levels and with different tissue specificities were established, m ...
DOI:
10.1007/s11248-007-9163-2
PubMed:
18097769

5. Visualisation of plastids in endosperm, pollen and roots of transgenic wheat expressing modified GFP fused to transit peptides from wheat SSU RubisCO, rice FtsZ and maize ferredoxin III proteins

Author:
Primavesi, Lucia F.; Wu, Huixia; Mudd, Elisabeth A.; Day, Anil; Jones, Huw D.
Source:
Transgenic research 2008 v.17 no.4 pp. 529-543
ISSN:
0962-8819
Subject:
actin; antibodies; chloroplasts; corn; endosperm; ferredoxins; fluorescence; leaves; peptides; pollen; protein synthesis; ribulose-bisphosphate carboxylase; rice; roots; tissues; transgenic plants; wheat
Abstract:
... The ability to target marker proteins to specific subcellular compartments is a powerful research tool to study the structure and development of organelles. Here transit sequences from nuclear-encoded, plastid proteins, namely rice FtsZ, maize non-photosynthetic ferredoxin III (FdIII) and the small subunit of RubisCO were used to target a modified synthetic GFP (S65G, S72A) to plastids. The locali ...
DOI:
10.1007/s11248-007-9126-7
PubMed:
17710559

6. Availability of subfertile transgenic rats expressing the c-myc gene as recipients for spermatogonial transplantation

Author:
Hirabayashi, Masumi; Yoshizawa, Yusuke; Kato, Megumi; Tsuchiya, Takashi; Nagao, Shizuko; Hochi, Shinichi
Source:
Transgenic research 2009 v.18 no.1 pp. 135-141
ISSN:
0962-8819
Subject:
actin; atrophy; busulfan; chickens; fluorescence; green fluorescent protein; humans; metallothionein; parturition; progeny; rats; seminiferous tubules; spermatids; spermatogenesis; spermatogonia; spermatozoa; stem cells; transgenes
Abstract:
... The spermatogonial transplantation system was applied to evaluate stem cell kinetics and niche quality and to produce gene-modified animals using the stem cells after homologous recombination-based selection. This study was designed to determine whether the transplanted spermatogonia were able to proliferate and differentiate in male rats expressing the c-myc transgene under control of the human m ...
DOI:
10.1007/s11248-008-9219-y
PubMed:
18830680

7. Mice with podocyte-specific overexpression of wild type α-actinin-4 are healthy controls for K256E-α-actinin-4 mutant transgenic mice

Author:
Michaud, Jean-Louis; Stitt-Cavanaugh, Erin; Endlich, Nicole; Endlich, Karlhans; De Repentigny, Yves; Kothary, Rashmi; Kennedy, Chris R. J.
Source:
Transgenic research 2010 v.19 no.2 pp. 285-289
ISSN:
0962-8819
Subject:
actin; antibodies; gene overexpression; hemagglutinins; kidneys; messenger RNA; mice; models; mutants; mutation; phenotype; polymerase chain reaction; transgenes
Abstract:
... Mutations in the gene ACTN4 encoding the actin bundling protein—α-actinin-4 underlie an inherited form of kidney lesions known as focal segmental glomerulosclerosis (FSGS). Previously, we developed a model for this condition by generating mice with podocyte-specific overexpression of a disease-causing mutant α-actinin-4 (K256E-ACTN4 pod⁺). However, whether α-actinin-4 overexpression artifacts and ...
DOI:
10.1007/s11248-009-9305-9

8. Temporally and spatially controlled expression of transgenes in embryonic and adult tissues

Author:
Zhang, Qian; Triplett, Aleata A.; Harms, Don W.; Lin, Wan-Chi; Creamer, Bradley A.; Rizzino, Angie; Wagner, Kay-Uwe
Source:
Transgenic research 2010 v.19 no.3 pp. 499-509
ISSN:
0962-8819
Subject:
actin; adults; bioluminescence; brain; chickens; disease course; doxycycline; embryonic stem cells; enzyme activity; heart; image analysis; ligands; mice; muscles; oncogenes; pancreas; tissues; transactivators; transcription (genetics); transcriptional activation; transgenes; transgenic animals
Abstract:
... Using ES cell-mediated transgenesis, we generated a novel mouse strain that permits a temporally and spatially controlled expression of responder genes in embryonic and multiple adult tissues. The transgene was constructed in a way that a CMV enhancer linked to the chicken β-actin promoter (CAG) drives the expression of the tetracycline-controlled transactivator (tTA) in particular tissues upon Cr ...
DOI:
10.1007/s11248-009-9329-1
PubMed:
19821046
PubMed Central:
PMC2866092

9. Development of transgenic zooplankton Artemia as a bioreactor to produce exogenous protein

Author:
Shih-Hung Chang; Ben-Chang Lee; Yan-Da Chen; Yin-Chou Lee; Huai-Jen Tsai
Source:
Transgenic research 2011 v.20 no.5 pp. 1099-1111
ISSN:
0962-8819
Subject:
Artemia; Danio rerio; actin; aquaculture feeds; body length; complementary DNA; gene transfer; nauplii; plasmids; porgy; reporter genes; zooplankton
Abstract:
... Although the crustacean Artemia has been commonly used as an experimental organism and served as a live bait feed for aquaculture, gene transfer system on Artemia sp. to generate stable lines is not well developed. In this study, we optimized a condition for cyst-eletroporation and generated stable lines of transgenic A. sinica. Two expression plasmids directed by the hybrid promoters of cytomegal ...
DOI:
10.1007/s11248-010-9479-1
PubMed:
21225340

10. Functional ability of cytoskeletal β-actin regulator to drive constitutive and ubiquitous expression of a fluorescent reporter throughout the life cycle of transgenic marine medaka Oryzias dancena

Author:
Young Sun Cho; Sang Yoon Lee; Youn Kyoung Kim; Dong Soo Kim; Yoon Kwon Nam
Source:
Transgenic research 2011 v.20 no.6 pp. 1333-1355
ISSN:
0962-8819
Subject:
Oryzias; actin; adults; bioinformatics; color; cytoskeleton; fluorescence; gene expression; genetically modified organisms; genotype; longevity; progeny; transcription factors; transgenes
Abstract:
... Marine medaka Oryzias dancena, a candidate model organism, represents many attractive merits as a material for experimental transgenesis and/or heterologous expression assay particularly in the field of ecotoxicology and developmental biology. In this study, cytoskeletal β-actin gene was characterized from O. dancena and the functional capability of its promoter to drive constitutive expression of ...
DOI:
10.1007/s11248-011-9501-2
PubMed:
21437716

11. Pathologic progression of mammary carcinomas in a C3(1)/SV40 T/t-antigen transgenic rat model of human triple-negative and Her2-positive breast cancer

Author:
M. J. Hoenerhoff; M. A. Shibata; A. Bode; J. E. Green
Source:
Transgenic research 2011 v.20 no.2 pp. 247-259
ISSN:
0962-8819
Subject:
actin; adenocarcinoma; animal models; breast neoplasms; epithelium; estrogen receptors; genetic engineering; humans; mammary glands; mammary neoplasms (animal); metastasis; mice; phenotype; progesterone receptors; rats; smooth muscle; transgenic animals
Abstract:
... The C3(1) component of the rat prostate steroid binding protein has been used to target expression of the SV40 T/t-antigen to the mammary epithelium of mice resulting in pre-neoplastic lesions that progress to invasive and metastatic cancer with molecular features of human basal-type breast cancer. However, there are major differences in the histologic architecture of the stromal and epithelial el ...
DOI:
10.1007/s11248-010-9406-5
PubMed:
20549348
PubMed Central:
PMC3103058

12. Expression of hGM-CSF in silk glands of transgenic silkworms using gene targeting vector

Author:
Renyu Xue; Huimei Chen; Linlin Cui; Guangli Cao; Wenlin Zhou; Xiaojian Zheng; Chengliang Gong
Source:
Transgenic research 2012 v.21 no.1 pp. 101-111
ISSN:
0962-8819
Subject:
Western blotting; actin; colony-stimulating factors; eggs; freeze drying; gene targeting; genes; humans; leukocyte count; mice; molecular weight; nucleic acid hybridization; polymerase chain reaction; silk; silk glands; silkworms
Abstract:
... The silk gland of the silkworm is a highly specialized organ that has the wonderful ability to synthesize and secrete silk protein. To express human granucyto-macrophage colony-stimulating factor (hGM-CSF) in the posterior silk glands of gene-targeted silkworms, a targeting vector pSK-FibL-L-A3GFP-PH-GMCSF-LPA-FibL-R was constructed, harboring a 1.2 kb portion of the left homogenous arm (FibL-L), ...
DOI:
10.1007/s11248-011-9513-y
PubMed:
21533901

13. Isolation of yellow catfish β-actin promoter and generation of transgenic yellow catfish expressing enhanced yellow fluorescent protein

Author:
Jiachun Ge; Zhangji Dong; Jingyun Li; Zhiqiang Xu; Wei Song; Jie Bao; Dong Liang; Junbo Li; Kui Li; Wenshuang Jia; Muzi Zhao; Yongxiang Cai; Jiaxin Yang; Jianlin Pan; Qingshun Zhao
Source:
Transgenic research 2012 v.21 no.5 pp. 995-1004
ISSN:
0962-8819
Subject:
Danio rerio; TATA box; Tachysurus fulvidraco; actin; aquaculture; early development; fish; genetic engineering; polymerase chain reaction; progeny; reporter genes; screening; start codon; transgenes; China
Abstract:
... Yellow catfish (Pelteobagrus fulvidraco Richardson) is one of the most important freshwater farmed species in China. However, its small size and slow growth rate limit its commercial value. Because genetic engineering has been a powerful tool to develop and improve fish traits for aquaculture, we performed transgenic research on yellow catfish in order to increase its size and growth rate. Perform ...
DOI:
10.1007/s11248-012-9606-2
PubMed:
22407406

14. Interaction of diet and the masou salmon Δ5-desaturase transgene on Δ6-desaturase and stearoyl-CoA desaturase gene expression and N-3 fatty acid level in common carp (Cyprinus carpio)

Author:
Qi Cheng; Baofeng Su; Zhenkui Qin; Chia-Chen Weng; Fang Yin; Yangen Zhou; Michael Fobes; Dayan A. Perera; Mei Shang; Fabio Soller; Zhiyi Shi; Allen Davis; Rex A. Dunham
Source:
Transgenic research 2014 v.23 no.5 pp. 729-742
ISSN:
0962-8819
Subject:
Cyprinus carpio; actin; brain; diet; gene expression; gene expression regulation; liver; messenger RNA; omega-3 fatty acids; salmon; stearoyl-CoA desaturase; transgenes
Abstract:
... The masou salmon Δ5-desaturase-like gene (D5D) driven by the common carp β-actin promoter was transferred into common carp (Cyprinus carpio) that were fed two diets. For P₁transgenic fish fed a commercial diet, Δ6-desaturase-like gene (D6D) and stearoyl-CoA desaturase (SCD) mRNA levels in muscle were up-regulated (P < 0.05) 12.7- and 17.9-fold, respectively, and the D6D mRNA level in the gonad of ...
DOI:
10.1007/s11248-014-9812-1
PubMed:
25011564

15. GH indirectly enhances the regeneration of transgenic zebrafish fins through IGF2a and IGF2b

Author:
Bruna Félix Nornberg; Daniela Volcan Almeida; Márcio Azevedo Figueiredo; Luis Fernando Marins
Source:
Transgenic research 2016 v.25 no.5 pp. 743-749
ISSN:
0962-8819
Subject:
Danio rerio; actin; amputation; fins; fish; gene overexpression; genetic engineering; models; promoter regions; receptors; somatomedins; somatotropin; tissue repair; transgenic animals
Abstract:
... The somatotropic axis, composed essentially of the growth hormone (GH) and insulin-like growth factors (IGFs), is the main regulator of somatic growth in vertebrates. However, these protein hormones are also involved in various other major physiological processes. Although the importance of IGFs in mechanisms involving tissue regeneration has already been established, little is known regarding the ...
DOI:
10.1007/s11248-016-9957-1

16. Highly efficient and inducible DNA excision in transgenic silkworms using the FLP/FRT site-specific recombination system

Author:
Dingpei Long; Weijian Lu; Zhanzhang Hao; Zhonghuai Xiang; Aichun Zhao
Source:
Transgenic research 2016 v.25 no.6 pp. 795-811
ISSN:
0962-8819
Subject:
DNA; Lepidoptera; actin; genes; genetic engineering; genetic markers; green fluorescent protein; heat stress; heat-shock protein 70; hybridization; messenger RNA; nucleotide sequences; progeny; silkworms; site-specific recombination; transgenic insects
Abstract:
... Efficient and inducible recombinase-mediated DNA excision is an optimal technology for automatically deleting unwanted DNA sequences, including selection marker genes. However, this methodology has yet to be established in transgenic silkworms. To achieve efficient and inducible FLP recombinase-mediated DNA excision in transgenic silkworms, one transgenic target strain (TTS) containing an FRT-flan ...
DOI:
10.1007/s11248-016-9970-4

17. Expression of endogenous proteins in maize hybrids in a multi-location field trial in India

Author:
Linga R. Gutha; Divakar Purushottam; Aruna Veeramachaneni; Sarita Tigulla; Vikas Kodappully; Chandana Enjala; Hitendrasinh Rajput; Jennifer Anderson; Bonnie Hong; Jean Schmidt; Shveta Bagga
Source:
Transgenic research 2018 v.27 no.4 pp. 331-342
ISSN:
0962-8819
Subject:
actin; corn; crops; developmental stages; enzyme-linked immunosorbent assay; field experimentation; genotype; glyceraldehyde-3-phosphate dehydrogenase; growing season; hybrids; leaves; models; peptide elongation factors; plant tissues; safety assessment; transgenic plants; India
Abstract:
... Genetically modified (GM) crops undergo large scale multi-location field trials to characterize agronomics, composition, and the concentration of newly expressed protein(s) [herein referred to as transgenic protein(s)]. The concentration of transgenic proteins in different plant tissues and across the developmental stages of the plant is considered in the safety assessment of GM crops. Reference o ...
DOI:
10.1007/s11248-018-0077-y

18. The intronic promoter of Actin4 mediates high-level transgene expression mainly in the wing and epidermis of silkworms

Author:
Tingting Tan; Rongpeng Liu; Qin Luo; Jingwen Ma; Yao Ou; Wenhui Zeng; Lichun Feng; Hanfu Xu
Source:
Transgenic research 2020 v.29 no.2 pp. 243-251
ISSN:
0962-8819
Subject:
Bombyx mori; Western blotting; actin; exons; fat body; fluorescence; gene expression; genetic engineering; introns; midgut; silkworms
Abstract:
... The cytoplasmic actin gene Actin4 (A4) in silkworm (Bombyx mori) was isolated 20 years ago and has a distal promoter upstream of the first exon and a proximal promoter within the first intron; however, how the promoter regulates gene expression has yet to be fully elucidated. Here, we characterized the function and expression of the proximal promoter (named A4IP) by analyzing transgenic Gal4/UAS s ...
DOI:
10.1007/s11248-020-00192-0

19. Direct and pleiotropic effects of the Masou Salmon Delta-5 Desaturase transgene in F1 channel catfish (Ictalurus punctatus)

Author:
Yingqi Huang; William Bugg; Max Bangs; Guyu Qin; David Drescher; Nathan Backenstose; Chia Chen Weng; Yiliu Zhang; Karim Khalil; Sheng Dong; Ahmed Elaswad; Zhi Ye; Cuiyu Lu; Khoi Vo; Rhoda Mae Simora; Xiaoli Ma; Zachary Taylor; Yujia Yang; Tao Zhou; Jingping Guo; Guillaume Salze; Zhenkui Qin; Yi Wang; Rex A. Dunham
Source:
Transgenic research 2021 v.30 no.2 pp. 185-200
ISSN:
0962-8819
Subject:
Cyprinus carpio; Ictalurus furcatus; Ictalurus punctatus; actin; body composition; disease resistance; fatty acid composition; genetic engineering; hybrids; males; nutritive value; omega-3 fatty acids; proximate composition; salmon; transgenes
Abstract:
... Channel catfish (Ictalurus punctatus) is the primary culture species in the US along with its hybrid made with male blue catfish, I. furcatus. In an effort to improve the nutritional value of channel catfish, the masou salmon Δ5-desaturase like gene (D5D) driven by the common carp beta-actin promoter (βactin) was inserted into channel catfish. The objectives of this study were to determine the eff ...
DOI:
10.1007/s11248-021-00242-1