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You searched for: Journal name Transgenic research Remove constraint Journal name: Transgenic research Subject term corn Remove constraint Subject term: corn
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1. A leaf-based regeneration and transformation system for maize (Zea mays L.)

Author:
Ahmadabadi, Mohammad; Ruf, Stephanie; Bock, Ralph
Source:
Transgenic research 2007 v.16 no.4 pp. 437-448
ISSN:
0962-8819
Subject:
Zea mays; callus; callus culture; corn; food crops; genetic engineering; genetic transformation; genetically modified organisms; greenhouses; in vitro studies; leaves; tissue culture
Abstract:
... Efficient methods for in vitro propagation, regeneration, and transformation of plants are of pivotal importance to both basic and applied research. While being the world's major food crops, cereals are among the most difficult-to-handle plants in tissue culture which severely limits genetic engineering approaches. In maize, immature zygotic embryos provide the predominantly used material for esta ...
DOI:
10.1007/s11248-006-9046-y
PubMed:
17103238

2. A proteomic-based approach to study underlying molecular responses of the small intestine of Wistar rats to genetically modified corn (MON810)

Author:
Asmaa AL-Harbi; Sahira Lary; Martin G. Edwards; Safaa Qusti; Andrew Cockburn; Morten Poulsen; Angharad M. R. Gatehouse
Source:
Transgenic research 2019 v.28 no.5-6 pp. 479-498
ISSN:
0962-8819
Subject:
Ostrinia nubilalis; biomarkers; carbohydrate metabolism; cell death; corn; diet; epithelium; gene expression regulation; genes; genetic engineering; insect resistance; insects; lipids; midgut; protein synthesis; proteomics; rats; safety assessment; small intestine; toxicity; toxicology; toxins
Abstract:
... A genetically modified (GM) commercial corn variety, MON810, resistant to European corn borer, has been shown to be non-toxic to mammals in a number of rodent feeding studies carried out in accordance with OECD Guidelines. Insect resistance results from expression of the Cry1Ab gene encoding an insecticidal Bt protein that causes lysis and cell death in susceptible insect larvae by binding to midg ...
DOI:
10.1007/s11248-019-00157-y
PubMed:
31172414
PubMed Central:
PMC6848250

3. A semi-quantitative approach to GMO risk-benefit analysis

Author:
E. Jane Morris
Source:
Transgenic research 2011 v.20 no.5 pp. 1055-1071
ISSN:
0962-8819
Subject:
biosafety; case studies; corn; decision support systems; developing countries; genetically modified organisms; insects; laws and regulations; precautionary principle; risk; risk-benefit analysis; South Africa
Abstract:
... In many countries there are increasing calls for the benefits of genetically modified organisms (GMOs) to be considered as well as the risks, and for a risk-benefit analysis to form an integral part of GMO regulatory frameworks. This trend represents a shift away from the strict emphasis on risks, which is encapsulated in the Precautionary Principle that forms the basis for the Cartagena Protocol ...
DOI:
10.1007/s11248-010-9480-8
PubMed:
21197601

4. A specific real-time quantitative PCR detection system for event MON810 in maize YieldGard based on the 3'-transgene integration sequence

Author:
Hernandez, M.; Pla, M.; Esteve, T.; Prat, S.; Puigdomenech, P.; Ferrando, A.
Source:
Transgenic research 2003 v.12 no.2 pp. 179-189
ISSN:
0962-8819
Subject:
Zea mays; corn; transgenic plants; polymerase chain reaction; synthetic genes; Bacillus thuringiensis; bacterial insecticides; endotoxins; bacterial toxins; crystal proteins; insect control; plasmid vectors
PubMed:
12739886

5. A study of crop-to-crop gene flow using farm scale sites of fodder maize (Zea mays L.) in the UK

Author:
Rebecca Weekes; Theodore Allnutt; Caroline Boffey; Sarah Morgan; Mark Bilton; Roger Daniels; Christine Henry
Source:
Transgenic research 2007 v.16 no.2 pp. 203-211
ISSN:
0962-8819
Subject:
Zea mays; corn; crops; farms; forage; gene flow; genes; guidelines; herbicide resistance; invertebrates; probability; quantitative polymerase chain reaction; statistical analysis; statistical models; transgenic plants; weeds; United Kingdom
Abstract:
... From 2000 to 2003 a range of Farm Scale Evaluation (FSE) trials were established in the UK to assess the effect of the release and management of herbicide tolerant (HT) crops on arable weeds and invertebrates. The FSE trials for maize were also used to investigate crop-to-crop gene flow and to develop a statistical model for the prediction of gene flow frequency that can be used to evaluate curren ...
DOI:
10.1007/s11248-006-9036-0
PubMed:
17115253

6. A transgenic approach to controlling wheat seed dormancy level by using Triticeae DOG1-like genes

Author:
Ikuo Ashikawa; Masahiko Mori; Shingo Nakamura; Fumitaka Abe
Source:
Transgenic research 2014 v.23 no.4 pp. 621-629
ISSN:
0962-8819
Subject:
Arabidopsis; RNA interference; agronomic traits; barley; corn; crops; cultivars; double-stranded RNA; germination; seed dormancy; transgenes; ubiquitin; wheat
Abstract:
... Seed dormancy is an important agronomic trait: low levels can cause premature germination, while too much can inhibit uniform germination. As an approach to controlling the seed dormancy level in crops, we used Triticeae DOG1-like genes as transgenes. DOG1 is an Arabidopsis gene that underlies natural variation in seed dormancy. We previously showed that although their sequence similarities to DOG ...
DOI:
10.1007/s11248-014-9800-5
PubMed:
24752830

7. A wheat genomic DNA fragment reduces pollen transmission of maize transgenes by reducing pollen viability

Author:
Scott, M. Paul; Peterson, Joan M.; Moran, Daniel L.; Sangtong, Varaporn; Smith, LaTrice
Source:
Transgenic research 2007 v.16 no.5 pp. 629-643
ISSN:
0962-8819
Subject:
viability; pollination; Zea mays; transgenes; transgenic plants; corn; Triticum aestivum; glufosinate; pollen germination; herbicide resistance; pollen
Abstract:
... A genomic DNA fragment from wheat carrying the Glu-1Dx5 gene has been shown to exhibit reduced pollen transmission in transgenic maize. To localize the region of the DNA fragment responsible for this reduced pollen transmission, we produced transgenic maize plants in which the wheat genomic DNA proximal to the 1Dx5 coding sequence was replaced with the maize 27 kDa γ-zein promoter. Like the wheat ...
Handle:
10113/3842
DOI:
10.1007/s11248-006-9055-x
PubMed:
17216545

8. Analysis of the maize polyubiquitin-1 promoter heat shock elements and generation of promoter variants with modified expression characteristics

Author:
Streatfield, S.J.; Magallanes-Lundback, M.E.; Beifuss, K.K.; Brooks, C.A.; Harkey, R.L.; Love, R.T.; Bray, J.; Howard, J.A.; Jilka, J.M.; Hood, E.E.
Source:
Transgenic research 2004 v.13 no.4 pp. 299-312
ISSN:
0962-8819
Subject:
Zea mays; corn; Zea diploperennis; promoter regions; mutation; ubiquitin; gene induction; heat stress; transgenic plants; transgenes; reporter genes; beta-glucuronidase; gene expression; seeds
DOI:
10.1023/B:TRAG.0000040053.23687.9c
PubMed:
15517990

9. Assessing the ecological risks from the persistence and spread of feral populations of insect-resistant transgenic maize

Author:
Alan Raybould; Laura S. Higgins; Michael J. Horak; Raymond J. Layton; Nicholas P. Storer; Juan Manuel De La Fuente; Rod A. Herman
Source:
Transgenic research 2012 v.21 no.3 pp. 655-664
ISSN:
0962-8819
Subject:
comparative risk assessment; corn; crops; field experimentation; risk communication; risk perception; transgenic plants
Abstract:
... One source of potential harm from the cultivation of transgenic crops is their dispersal, persistence and spread in non-agricultural land. Ecological damage may result from such spread if the abundance of valued species is reduced. The ability of a plant to spread in non-agricultural habitats is called its invasiveness potential. The risks posed by the invasiveness potential of transgenic crops ar ...
DOI:
10.1007/s11248-011-9560-4
PubMed:
22002083
PubMed Central:
PMC3348485

10. Assessing the transfer of genetically modified DNA from feed to animal tissues

Author:
Mazza, R.; Soave, M.; Morlacchini, M.; Piva, G.; Marocco, A.
Source:
Transgenic research 2005 v.14 no.5 pp. 775-784
ISSN:
0962-8819
Subject:
insecticidal proteins; pork; Zea mays; recombinant DNA; transgenes; feeds; swine feeding; transgenic plants; polymerase chain reaction; corn; delta-endotoxins; risk assessment; Bacillus thuringiensis; crystal proteins; zein; food safety; human food chain; animal tissues; traceability
DOI:
10.1007/s11248-005-0009-5
PubMed:
16245168

11. Assessment of Real-time PCR Based Methods for Quantification of Pollen-mediated Gene Flow from GM to Conventional Maize in a Field Study

Author:
Pla, M.; La Paz, J.L.; Peñas, G.; García, N.; Palaudelmàs, M.; Esteve, T.; Messeguer, J.; Melé, E.
Source:
Transgenic research 2006 v.15 no.2 pp. 219-228
ISSN:
0962-8819
Subject:
Zea mays; corn; transgenic plants; gene flow; pollen; pollen flow; polymerase chain reaction; introgression; risk assessment; cross pollination; transgenes; Bacillus thuringiensis; crystal proteins; insecticidal proteins; delta-endotoxins; Spain
DOI:
10.1007/s11248-005-4945-x
PubMed:
16604462

12. Assessment of field-grown cellulase-expressing corn

Author:
Martina Garda; Shivakumar P. Devaiah; Deborah Vicuna Requesens; Yeun-Kyung Chang; Audrei Dabul; Christy Hanson; Kendall R. Hood; Elizabeth E. Hood
Source:
Transgenic research 2015 v.24 no.2 pp. 185-198
ISSN:
0962-8819
Subject:
Food and Drug Administration; USDA; Zea mays; compliance; corn; drugs; endo-1,4-beta-glucanase; environmental protection; genes; genetic engineering; hybrids; industrial crops; pesticides; transgenic plants; United States
Abstract:
... Transgenic plants in the US and abroad generated using genetic engineering technology are regulated with respect to release into the environment and inclusion into diets of humans and animals. For crops incorporating pharmaceuticals or industrial enzymes regulations are even more stringent. Notifications are not allowed for movement and release, therefore a permit is required. However, growing und ...
DOI:
10.1007/s11248-014-9838-4

13. Assessment of potential impacts associated with gene flow from transgenic hybrids to Mexican maize landraces

Author:
Bill Duncan; Elisa Leyva-Guerrero; Todd Werk; Duška Stojšin; Baltazar M. Baltazar; Silverio García-Lara; Mariana Zavala-López; Juan Manuel de la Fuente-Martínez; Chen Meng
Source:
Transgenic research 2019 v.28 no.5-6 pp. 509-523
ISSN:
0962-8819
Subject:
backcrossing; center of origin; commercialization; corn; gene flow; genetic engineering; herbicide resistance; insects; introgression; landraces; phenotype; plant response; seeds; transgenes; Mexico
Abstract:
... Genetically modified (GM) maize has been grown and safely consumed on a global scale since its commercialization in 1996. However, questions have been raised about the potential impact that GM maize could have on native maize landraces in Mexico, which is the center of origin and diversity of maize. This research was conducted to evaluate potential changes to maize landraces in an unlikely event o ...
DOI:
10.1007/s11248-019-00160-3
PubMed:
31250247
PubMed Central:
PMC6848245

14. Assessment of the influence of field size on maize gene flow using SSR analysis

Author:
M. Palaudelmàs; E. Melé; A. Monfort; J. Serra; J. Salvia; J. Messeguer
Source:
Transgenic research 2012 v.21 no.3 pp. 471-483
ISSN:
0962-8819
Subject:
corn; gene flow; genetically modified organisms; microsatellite repeats; pollen; pollen flow; seeds
Abstract:
... One of the factors that may influence the rate of cross-fertilization is the relative size of the pollen donor and receptor fields. We designed a spatial distribution with four varieties of genetically-modified (GM) yellow maize to generate different sized fields while maintaining a constant distance to neighbouring fields of conventional white kernel maize. Samples of cross-fertilized, yellow ker ...
DOI:
10.1007/s11248-011-9549-z
PubMed:
21898271

15. Assessment of the potential for gene flow from transgenic maize (Zea mays L.) to eastern gamagrass (Tripsacum dactyloides L.)

Author:
Moon-Sub Lee; Eric K. Anderson; Duška Stojšin; Marc A. McPherson; Baltazar Baltazar; Michael J. Horak; Juan Manuel de la Fuente; Kunsheng Wu; James H. Crowley; A. Lane Rayburn; D. K. Lee
Source:
Transgenic research 2017 v.26 no.4 pp. 501-514
ISSN:
0962-8819
Subject:
Tripsacum dactyloides; Zea mays; corn; cross pollination; environmental assessment; females; gene flow; genetic engineering; glyphosate; habitats; herbicide resistance; hybrids; inflorescences; interspecific hybridization; males; pollen; polymerase chain reaction; risk; risk assessment; seedlings; seeds; transgenes; transgenic plants; wild relatives; Illinois
Abstract:
... Eastern gamagrass (Tripsacum dactyloides L.) belongs to the same tribe of the Poaceae family as maize (Zea mays L.) and grows naturally in the same region where maize is commercially produced in the USA. Although no evidence exists of gene flow from maize to eastern gamagrass in nature, experimental crosses between the two species were produced using specific techniques. As part of environmental r ...
DOI:
10.1007/s11248-017-0020-7
PubMed:
28466411
PubMed Central:
PMC5504203

16. Biodegradation of atrazine by three transgenic grasses and alfalfa expressing a modified bacterial atrazine chlorohydrolase gene

Author:
Andrew W. Vail; Ping Wang; Hirotaka Uefuji; Deborah A. Samac; Carroll P. Vance; Lawrence P. Wackett; Michael J. Sadowsky
Source:
Transgenic research 2015 v.24 no.3 pp. 475-488
ISSN:
0962-8819
Subject:
atrazine; alfalfa; promoter regions; metabolites; Panicum virgatum; weed control; Festuca arundinacea; hydroponics; gene expression; Nicotiana tabacum; introns; alcohol dehydrogenase; grasses; Cassava vein mosaic virus; Zea mays; atrazine chlorohydrolase; Lolium perenne; corn; herbicide resistance; ubiquitin; Pseudomonas; tobacco; transgenic plants; Medicago sativa; biodegradation
Abstract:
... The widespread use of atrazine and other s-triazine herbicides to control weeds in agricultural production fields has impacted surface and groundwater in the United States and elsewhere. We previously reported the cloning, sequencing, and expression of six genes involved in the atrazine biodegradation pathway of Pseudomonas sp. strain ADP, which is initiated by atzA, encoding atrazine chlorohydrol ...
Handle:
10113/60882
DOI:
10.1007/s11248-014-9851-7

17. Bt-maize event MON 88017 expressing Cry3Bb1 does not cause harm to non-target organisms

Author:
Yann Devos; Adinda De Schrijver; Patrick De Clercq; József Kiss; Jörg Romeis
Source:
Transgenic research 2012 v.21 no.6 pp. 1191-1214
ISSN:
0962-8819
Subject:
Chrysomelidae; adults; adverse effects; corn; host plants; insecticidal properties; larvae; nontarget organisms; pollen; risk; rootworms
Abstract:
... This review paper explores whether the cultivation of the genetically modified Bt-maize transformation event MON 88017, expressing the insecticidal Cry3Bb1 protein against corn rootworms (Coleoptera: Chrysomelidae), causes adverse effects to non-target organisms (NTOs) and the ecological and anthropocentric functions they provide. Available data do not reveal adverse effects of Cry3Bb1 on various ...
DOI:
10.1007/s11248-012-9617-z
PubMed:
22576225

18. Characterization of grape Gibberellin Insensitive1 mutant alleles in transgenic Arabidopsis

Author:
Gan-Yuan Zhong; Yingzhen Yang
Source:
Transgenic research 2012 v.21 no.4 pp. 725-741
ISSN:
0962-8819
Subject:
Arabidopsis; Brassica; alleles; barley; branches; corn; gibberellins; grapes; growth and development; inflorescences; internodes; leaves; mutants; plant architecture; plant growth; pods; seeds; wheat
Abstract:
... We generated 12 different mutations in the grape Gibberellin Insensitive1 (VvGAI1) sequences, transformed them into Arabidopsis under the control of 35S, Arabidopsis GAI or grape GAI1 promoter, and evaluated the impact of these mutant alleles on plant growth and development. These VvGAI1 sequence variants included some mimics of the known GAI-like mutant alleles discovered in grape, wheat, barley, ...
DOI:
10.1007/s11248-011-9565-z
PubMed:
22038449

19. Cloning and functional characterization of the maize carotenoid isomerase and β-carotene hydroxylase genes and their regulation during endosperm maturation

Author:
Li, Qunrui; Farre, Gemma; Naqvi, Shaista; Breitenbach, Jürgen; Sanahuja, Georgina; Bai, Chao; Sandmann, Gerhard; Capell, Teresa; Christou, Paul; Zhu, Changfu
Source:
Transgenic research 2010 v.19 no.6 pp. 1053-1068
ISSN:
0962-8819
Subject:
enzymes; beta-cryptoxanthin; genetically modified organisms; lycopene; zeaxanthin; gene expression; beta-carotene; introns; stop codon; cultivars; Zea mays; messenger RNA; complementary DNA; chromosomes; corn; mutation; lutein; inbred lines; biochemical pathways; endosperm
Abstract:
... In order to gain further insight into the partly-characterized carotenoid biosynthetic pathway in corn (Zea mays L.), we cloned cDNAs encoding the enzymes carotenoid isomerase (CRTISO) and β-carotene hydroxylase (BCH) using endosperm mRNA isolated from inbred line B73. For both enzymes, two distinct cDNAs were identified mapping to different chromosomes. The two crtiso cDNAs (Zmcrtiso1 and Zmcrtis ...
DOI:
10.1007/s11248-010-9381-x

20. Co-expression of a modified maize ribosome-inactivating protein and a rice basic chitinase gene in transgenic rice plants confers enhanced resistance to sheath blight

Author:
Kim, J.K.; Jang, I.C.; Wu, R.; Zuo, W.N.; Boston, R.S.; Lee, Y.H.; Ahn, I.P.; Nahm, B.H.
Source:
Transgenic research 2003 v.12 no.4 pp. 475-484
ISSN:
0962-8819
Subject:
rice; Oryza sativa; plant pathogenic fungi; Magnaporthe grisea; blight; genetic resistance; Bipolaris oryzae; fungal diseases of plants; Thanatephorus cucumeris; gene expression; protein synthesis inhibitors; disease resistance; gene segregation; Zea mays; corn; N-glycoside hydrolases; genetic transformation; biolistics; transgenes; chitinase
PubMed:
12885168

21. Coexpression of octopine and succinamopine Agrobacterium virulence genes to generate high quality transgenic events in maize by reducing vector backbone integration

Author:
Nagesh Sardesai; Stephen Foulk; Wei Chen; Huixia Wu; Emily Etchison; Manju Gupta
Source:
Transgenic research 2018 v.27 no.6 pp. 539-550
ISSN:
0962-8819
Subject:
corn; genetic engineering; octopine; operon; plasmids; toxins; virulence
Abstract:
... Agrobacterium-mediated transformation is a complex process that is widely utilized for generating transgenic plants. However, one of the major concerns of this process is the frequent presence of undesirable T-DNA vector backbone sequences in the transgenic plants. To mitigate this deficiency, a ternary strain of A. tumefaciens was modified to increase the precision of T-DNA border nicking such th ...
DOI:
10.1007/s11248-018-0097-7

22. Comparison of droplet digital PCR with quantitative real-time PCR for determination of zygosity in transgenic maize

Author:
Xiaoli Xu; Cheng Peng; Xiaofu Wang; Xiaoyun Chen; Qiang Wang; Junfeng Xu
Source:
Transgenic research 2016 v.25 no.6 pp. 855-864
ISSN:
0962-8819
Subject:
DNA; corn; quantitative polymerase chain reaction; transgenes; transgenic plants
Abstract:
... This study evaluated the applicability of droplet digital PCR (ddPCR) as a tool for maize zygosity determination using quantitative real-time PCR (qPCR) as a reference technology. Quantitative real-time PCR is commonly used to determine transgene copy number or GMO zygosity characterization. However, its effectiveness is based on identical reaction efficiencies for the transgene and the endogenous ...
DOI:
10.1007/s11248-016-9982-0

23. Consideration of familiarity accumulated in the confined field trials for environmental risk assessment of genetically modified soybean (Glycine max) in Japan

Author:
Akane Matsushita; Hidetoshi Goto; Yasuyuki Takahashi; Mai Tsuda; Ryo Ohsawa
Source:
Transgenic research 2020 v.29 no.2 pp. 229-242
ISSN:
0962-8819
Subject:
Glycine max; Japan; canola; corn; cotton; data collection; environmental assessment; genetic engineering; information sources; risk; soybeans
Abstract:
... To date, there have been 160 regulatory approvals for environmental safety in Japan for the major genetically modified (GM) crops, including corn, soybean, canola and cotton. Confined field trials (CFTs) have been conducted in Japan for all single events, which contain various traits. The accumulated information from these previously conducted CFTs, as well as the agronomic field study data from o ...
DOI:
10.1007/s11248-020-00193-z
PubMed:
31997144
PubMed Central:
PMC7067755

24. Crop plants with improved culture and quality traits for food, feed and other uses

Author:
Fabien Nogué; Philippe Vergne; Anne-Marie Chèvre; Jean-Eric Chauvin; Oumaya Bouchabké-Coussa; Annabelle Déjardin; Elisabeth Chevreau; Laurence Hibrand-Saint Oyant; Marianne Mazier; Pierre Barret; Emmanuel Guiderdoni; Christophe Sallaud; Séverine Foucrier; Pierre Devaux; Peter M. Rogowsky
Source:
Transgenic research 2019 v.28 no.Supplement 2 pp. 65-73
ISSN:
0962-8819
Subject:
Arabidopsis; Brachypodium; Brassica napus; Physcomitrella; Populus; apples; bioeconomics; corn; disease resistance; genome; horses; plant architecture; plant reproduction; potatoes; research projects; rice; salt tolerance; starch; sustainable agriculture; tomatoes; viruses; wheat
Abstract:
... The large French research project GENIUS (2012–2019, https://www6.inra.genius-project_eng/) provides a good showcase of current genome editing techniques applied to crop plants. It addresses a large variety of agricultural species (rice, wheat, maize, tomato, potato, oilseed rape, poplar, apple and rose) together with some models (Arabidopsis, Brachypodium, Physcomitrella). Using targeted mutagene ...
DOI:
10.1007/s11248-019-00135-4

25. Decomposition dynamics and structural plant components of genetically modified Bt maize leaves do not differ from leaves of conventional hybrids

Author:
Zurbrügg, Corinne; Hönemann, Linda; Meissle, Michael; Romeis, Jörg; Nentwig, Wolfgang
Source:
Transgenic research 2010 v.19 no.2 pp. 257-267
ISSN:
0962-8819
Subject:
Bacillus thuringiensis; adverse effects; carbon nitrogen ratio; cellulose; corn; field experimentation; growing season; hemicellulose; hybrids; leaves; plant residues; soil ecology
Abstract:
... The cultivation of genetically modified Bt maize has raised environmental concerns, as large amounts of plant residues remain in the field and may negatively impact the soil ecosystem. In a field experiment, decomposition of leaf residues from three genetically modified (two expressing the Cry1Ab, one the Cry3Bb1 protein) and six non-transgenic hybrids (the three corresponding non-transformed near ...
DOI:
10.1007/s11248-009-9304-x
PubMed:
19609704

26. Definition and feasibility of isolation distances for transgenic maize cultivation

Author:
Olivier Sanvido; Franco Widmer; Michael Winzeler; Bernhard Streit; Erich Szerencsits; Franz Bigler
Source:
Transgenic research 2008 v.17 no.3 pp. 317-335
ISSN:
0962-8819
Subject:
European Union; aerial photography; agricultural land; corn; crop production; crops; geographic information systems; mixing; production technology; scientists; silage; transgenic plants; Europe
Abstract:
... A major concern related to the adoption of genetically modified (GM) crops in agricultural systems is the possibility of unwanted GM inputs into non-GM crop production systems. Given the increasing commercial cultivation of GM crops in the European Union (EU), there is an urgent need to define measures to prevent mixing of GM with non-GM products during crop production. Cross-fertilization is one ...
DOI:
10.1007/s11248-007-9103-1
PubMed:
17562214

27. Degradation of Cry1Ab protein from genetically modified maize (MON810) in relation to total dietary feed proteins in dairy cow digestion

Author:
Paul, Vijay; Guertler, Patrick; Wiedemann, Steffi; Meyer, Heinrich H. D.
Source:
Transgenic research 2010 v.19 no.4 pp. 683-689
ISSN:
0962-8819
Subject:
Simmental; abomasum; cecum; corn; crystal proteins; dairy cows; digesta; digestion; enzyme-linked immunosorbent assay; feces; insecticidal proteins; lactation; protein content; rumen; slaughter; small intestine; total mixed rations; transgenic plants
Abstract:
... To investigate the relative degradation and fragmentation pattern of the recombinant Cry1Ab protein from genetically modified (GM) maize MON810 throughout the gastrointestinal tract (GIT) of dairy cows, a 25 months GM maize feeding study was conducted on 36 lactating Bavarian Fleckvieh cows allocated into two groups (18 cows per group) fed diets containing either GM maize MON810 or nearly isogenic ...
DOI:
10.1007/s11248-009-9339-z
PubMed:
19888668
PubMed Central:
PMC2902738

28. Degradation of transgenic Bacillus thuringiensis proteins in corn tissue in response to post-harvest management practices

Author:
V. Yurchak; A. W. Leslie; G. P. Dively; W. O. Lamp; C. R. R. Hooks
Source:
Transgenic research 2021 v.30 no.6 pp. 851-865
ISSN:
0962-8819
Subject:
Bacillus thuringiensis; Ostrinia nubilalis; bioactive properties; biodegradation; corn; cover crops; crop residues; genetic engineering; no-tillage; postharvest technology; risk; soil; temperature
Abstract:
... Knowledge of the persistence of Cry proteins in transgenic corn residue after harvest is necessary to assess the ecological risk to nontarget organisms. The amount of protein remaining in crop residue declines over time by a combination of microbial decomposition and leaching, both influenced by temperature, precipitation, and the amount of residue-soil contact. Here, we investigated how long biol ...
DOI:
10.1007/s11248-021-00273-8

29. Determination of insecticidal Cry1Ab protein in soil collected in the final growing seasons of a nine-year field trial of Bt-maize MON810

Author:
Helga Gruber; Vijay Paul; Heinrich H. D. Meyer; Martin Müller
Source:
Transgenic research 2012 v.21 no.1 pp. 77-88
ISSN:
0962-8819
Subject:
corn; field experimentation; growing season; plant residues; root exudates; soil sampling; spring; topsoil
Abstract:
... Cultivation of genetically modified maize (Bt-maize; event MON810) producing recombinant δ-endotoxin Cry1Ab, leads to introduction of the insecticidal toxin into soil by way of root exudates and plant residues. This study investigated the fate of Cry1Ab in soil under long-term Bt-maize cultivation in an experimental field trial performed over nine growing seasons on four South German field sites c ...
DOI:
10.1007/s11248-011-9509-7
PubMed:
21499757

30. Development of a tightly regulated and highly inducible ecdysone receptor gene switch for plants through the use of retinoid X receptor chimeras

Author:
Tavva, Venkata S.; Dinkins, Randy D.; Palli, Subba R.; Collins, Glenn B.
Source:
Transgenic research 2007 v.16 no.5 pp. 599-612
ISSN:
0962-8819
Subject:
Nicotiana tabacum; tobacco; Zea mays; corn; Glycine max; soybeans; protoplasts; transgenic plants; hormone receptors; transcription factors; humans; Locusta migratoria; Choristoneura fumiferana; recombinant proteins; gene expression; gene expression regulation; methoxyfenozide; reporter genes; luciferase; genetic transformation
Abstract:
... Chemical inducible gene regulation systems provide essential tools for the precise regulation of transgene expression in plants and animals. Recent development of a two-hybrid ecdysone receptor (EcR) gene regulation system has solved some of the drawbacks that were associated with the monopartate gene switch. To further improve the versatility of the two-hybrid EcR gene switch for wide spread use ...
Handle:
10113/3841
DOI:
10.1007/s11248-006-9054-y
PubMed:
17139530

31. Development of dwarfish and yield-effective GM maize through passivation of bioactive gibberellin

Author:
Ziqi Chen; Yang Liu; Yuejia Yin; Qing Liu; Nan Li; Xiangguo Liu; Xia Li; Changhong Guo; Dongyun Hao
Source:
Transgenic research 2019 v.28 no.5-6 pp. 589-599
ISSN:
0962-8819
Subject:
chlorophyll; corn; dwarf cultivars; field experimentation; genes; genetic engineering; grain yield; harvest index; oxygenases; plant height; rice; wheat
Abstract:
... During the Green Revolution in the 1960s, breeding dwarf cultivars turned out to be a landmark, leading to a significant increase in the global production of wheat and rice. The most direct and effective strategy for breeding dwarf crops, among others, would be to control endogenous gibberellin (GA) levels of the crops. GA 2-oxidases are a group of 2-oxoglutarate-dependent dioxygenases that cataly ...
DOI:
10.1007/s11248-019-00172-z

32. Development of real time PCR assays for detection and quantification of transgene DNA of a Bacillus thuringiensis (Bt) corn hybrid in soil samples

Author:
Zhu, Bin; Ma, Bao-Luo; Blackshaw, Robert E.
Source:
Transgenic research 2010 v.19 no.5 pp. 765-774
ISSN:
0962-8819
Subject:
Bacillus thuringiensis; DNA; Pseudomonas stutzeri; Zea mays; antibiotic resistance; corn; crop rotation; field experimentation; growing season; horizontal gene transfer; hybrids; kanamycin; kanamycin kinase; quantitative polymerase chain reaction; risk; soil; soil sampling; soybeans; transgenes; transgenic plants
Abstract:
... Real time PCR assays were developed to detect and quantify the transgene DNA of a commercially released Bacillus thuringiensis (Bt) corn (Zea mays L.) hybrid (DKC42-23), which was derived from the event MON863 and also carried a neomycin phosphotransferase gene (the nptII gene). We applied the real time PCR assays to investigate the persistence of the transgene DNA in a field trial grown with DKC4 ...
DOI:
10.1007/s11248-009-9353-1
PubMed:
20044792

33. Effect of promoter driving selectable marker on corn transformation

Author:
Shiva Prakash, N.; Prasad, V.; Chidambram, Thillai P.; Cherian, Shoba; Jayaprakash, T. L.; Dasgupta, Santanu; Wang, Qi; Mann, Michael T.; Spencer, T. Michael; Boddupalli, Raghava S.
Source:
Transgenic research 2008 v.17 no.4 pp. 695-704
ISSN:
0962-8819
Subject:
corn; genetic markers; genetically modified organisms; reporter genes; rice; transgenes
Abstract:
... Identification of an appropriate selection agent and its corresponding selectable marker gene is one of the first steps in establishing a transformation protocol for a given plant species. As the promoter controls expression level of the genes, the promoter driving the selectable marker gene can affect transformation. However, investigations into the direct effect of promoters driving selectable m ...
DOI:
10.1007/s11248-007-9149-0
PubMed:
17952623

34. Effect of volunteers on maize gene flow

Author:
Palaudelmàs, Montserrat; Peñas, Gisela; Melé, Enric; Serra, Joan; Salvia, Jordi; Pla, Maria; Nadal, Anna; Messeguer, Joaquima
Source:
Transgenic research 2009 v.18 no.4 pp. 583-594
ISSN:
0962-8819
Subject:
corn; gene flow; genetically modified organisms; pollen; temperate zones; volunteer plants
Abstract:
... Regulatory approvals for deliberate release of GM maize events into the environment have lead to real situations of coexistence between GM and non-GM, with some fields being cultivated with GM and conventional varieties in successive seasons. Given the common presence of volunteer plants in maize fields in temperate areas, we investigated the real impact of GM volunteers on the yield of 12 non-GM ...
DOI:
10.1007/s11248-009-9250-7
PubMed:
19225900

35. Effects of Bt maize on the herbivore Spodoptera littoralis (Lepidoptera: Noctuidae) and the parasitoid Cotesia marginiventris (Hymenoptera: Braconidae)

Author:
Vojtech, E.; Meissle, M.; Poppy, G.M.
Source:
Transgenic research 2005 v.14 no.2 pp. 133-144
ISSN:
0962-8819
Subject:
Zea mays; corn; transgenic plants; insect pests; Spodoptera littoralis; crop damage; tritrophic interactions; larvae; transgenes; gene expression; crystal proteins; insecticidal proteins; nontarget organisms; Cotesia marginiventris; parasitoids; predator-prey relationships; feeding preferences; bioassays; natural enemies; mortality; insect development
DOI:
10.1007/s11248-005-2736-z
PubMed:
16022385

36. Effects of Bt maize-fed prey on the generalist predator Poecilus cupreus L. (Coleoptera: Carabidae)

Author:
Meissle, M.; Vojtech, E; Poppy, G.M.
Source:
Transgenic research 2005 v.14 no.2 pp. 123-132
ISSN:
0962-8819
Subject:
Zea mays; corn; transgenic plants; insect pests; Spodoptera littoralis; crop damage; tritrophic interactions; transgenes; gene expression; crystal proteins; insecticidal proteins; nontarget organisms; Pterostichus cupreus; predatory insects; predator-prey relationships; feeding preferences; bioassays; pupae; larvae; mortality
DOI:
10.1007/s11248-004-6458-4
PubMed:
16022384

37. Effects of transgenic cry1Ie maize on non-lepidopteran pest abundance, diversity and community composition

Author:
Jingfei Guo; Kanglai He; Shuxiong Bai; Tiantao Zhang; Yunjun Liu; Fuxin Wang; Zhenying Wang
Source:
Transgenic research 2016 v.25 no.6 pp. 761-772
ISSN:
0962-8819
Subject:
Bacillus thuringiensis; community structure; corn; multidimensional scaling; pests; proteins; species diversity; toxins; transgenic plants; variance
Abstract:
... Non-lepidopteran pests are exposed to, and may be influenced by, Bt toxins when feeding on Bt maize that express insecticidal Cry proteins derived from Bacillus thuringiensis (Bt). In order to assess the potential effects of transgenic cry1Ie maize on non-lepidopteran pest species and ecological communities, a 2-year field study was conducted to compare the non-lepidopteran pest abundance, diversi ...
DOI:
10.1007/s11248-016-9968-y

38. Eliminating host-mediated effects demonstrates Bt maize producing Cry1F has no adverse effects on the parasitoid Cotesia marginiventris

Author:
Steve E Naranjo
Source:
Transgenic research 2014 v.23 pp. 257-264
ISSN:
0962-8819
Subject:
Bacillus thuringiensis; Cotesia marginiventris; Spodoptera frugiperda; adults; adverse effects; cocoons; corn; fecundity; hosts; insects; larvae; leaves; longevity; parasitism; parasitoids; proteins; sex ratio; survival rate; Puerto Rico; United States
Abstract:
... The fall armyworm, Spodoptera frugiperda, is an important pest of maize in the United States and many tropical areas in the western hemisphere. In 2001, Herculex I ® (Cry1F) maize was commercially planted in the United States to control Lepidoptera, including S. frugiperda. In 2006, a population of S. frugiperda was discovered in Puerto Rico that had evolved resistance to Cry1F maize in the field, ...
Handle:
10113/58931
DOI:
10.1007/s11248-013-9748-x
PubMed:
24026808

39. Engineered maize as a source of astaxanthin: processing and application as fish feed

Author:
Jürgen Breitenbach; Marilise Nogueira; Gemma Farré; Changfu Zhu; Teresa Capell; Paul Christou; Gunther Fleck; Ulfert Focken; Paul D. Fraser; Gerhard Sandmann
Source:
Transgenic research 2016 v.25 no.6 pp. 785-793
ISSN:
0962-8819
Subject:
Oncorhynchus mykiss; astaxanthin; color; corn; enantiomers; ethanol; feces; feed formulation; feed supplements; fish feeds; fish fillets; oils; pigmentation; raw materials; transgenic plants; trout; zeaxanthin
Abstract:
... Astaxanthin from a transgenic maize line was evaluated as feed supplement source conferring effective pigmentation of rainbow trout flesh. An extraction procedure using ethanol together with the addition of vegetal oil was established. This resulted in an oily astaxanthin preparation which was not sufficiently concentrated for direct application to the feed. Therefore, a concentration process invo ...
DOI:
10.1007/s11248-016-9971-3

40. Enhanced conversion of plant biomass into glucose using transgenic rice-produced endoglucanase for cellulosic ethanol

Author:
Oraby, Hesham; Venkatesh, Balan; Dale, Bruce; Ahmad, Rashid; Ransom, Callista; Oehmke, James; Sticklen, Mariam
Source:
Transgenic research 2007 v.16 no.6 pp. 739-749
ISSN:
0962-8819
Subject:
Acidothermus cellulolyticus; active sites; ammonia; bioethanol; biomass; burning; cellulose; corn; endo-1,4-beta-glucanase; food crops; gene expression; genes; genetically modified organisms; glucose; growth and development; hydrolysis; plant growth; proteins; rice; rice straw; sustainable technology; thermal stability
Abstract:
... The catalytic domain of Acidothermus cellulolyticus thermostable endoglucanase gene (encoding for endo-1,4-β-glucanase enzyme or E1) was constitutively expressed in rice. Molecular analyses of T1 plants confirmed presence and expression of the transgene. The amount of E1 enzyme accounted for up to 4.9% of the plant total soluble proteins, and its accumulation had no apparent deleterious effects on ...
DOI:
10.1007/s11248-006-9064-9
PubMed:
17237981

41. Enhanced single copy integration events in corn via particle bombardment using low quantities of DNA

Author:
Lowe, Brenda A.; Shiva Prakash, N.; Way, Melissa; Mann, Michael T.; Spencer, T. Michael; Boddupalli, Raghava S.
Source:
Transgenic research 2009 v.18 no.6 pp. 831-840
ISSN:
0962-8819
Subject:
corn; cultivars; gene expression; plasmids; screening; statistical analysis; transgenes
Abstract:
... Transgene copy number is an important criterion for determining the utility of transgenic events. Single copy integration events are highly desirable when the objective is to produce marker free plants through segregation or when it is necessary to introgress different transgenes into commercial cultivars from different transgenic events. In contrast multi-copy events are advocated by several auth ...
DOI:
10.1007/s11248-009-9265-0
PubMed:
19381853

42. Environmental impact of herbicide regimes used with genetically modified herbicide-resistant maize

Author:
Devos, Yann; Cougnon, Mathias; Vergucht, Sofie; Bulcke, Robert; Haesaert, Geert; Steurbaut, Walter; Reheul, Dirk
Source:
Transgenic research 2008 v.17 no.6 pp. 1059-1077
ISSN:
0962-8819
Subject:
European Union; Zea mays; active ingredients; acute toxicity; agricultural land; aquatic organisms; biodiversity; corn; crop production; crops; environmental impact; food loss; glyphosate; groundwater; herbicide resistance; human health; pesticide application; risk; transgenic plants; weed control; weeds; Belgium
Abstract:
... With the potential advent of genetically modified herbicide-resistant (GMHR) crops in the European Union, changes in patterns of herbicide use are predicted. Broad-spectrum, non-selective herbicides used with GMHR crops are expected to substitute for a set of currently used herbicides, which might alter the agro-environmental footprint from crop production. To test this hypothesis, the environment ...
DOI:
10.1007/s11248-008-9181-8
PubMed:
18404410

43. Establishment and optimization of a regionally applicable maize gene-flow model

Author:
Ning Hu; Jichao Hu; Xiaodong Jiang; Zongzhi Lu; Yufa Peng; Wanlong Chen; Kemin Yao; Ming Zhang; Shirong Jia; Xinwu Pei; Weihong Luo
Source:
Transgenic research 2014 v.23 no.5 pp. 795-807
ISSN:
0962-8819
Subject:
corn; field experimentation; gene flow; models; outcrossing; pollen; prediction; seed quality; transgenes; China
Abstract:
... Because of the rapid development of transgenic maize, the potential effect of transgene flow on seed purity has become a major concern in public and scientific communities. Setting a proper isolation distance in field experiments and seed production is a possible solution to meet seed-quality standards and ensure adventitious contamination of products is below a specific threshold. By using a Gaus ...
DOI:
10.1007/s11248-014-9810-3
PubMed:
24962816

44. Establishment of a novel, eco-friendly transgenic pig model using porcine pancreatic amylase promoter-driven fungal cellulase transgenes

Author:
Y. S. Lin; C. C. Yang; C. C. Hsu; J. T. Hsu; S. C. Wu; C. J. Lin; W. T. K. Cheng
Source:
Transgenic research 2015 v.24 no.1 pp. 61-71
ISSN:
0962-8819
Subject:
Southern blotting; byproducts; corn; diet; endo-1,4-beta-glucanase; enzyme activity; fiber content; fungi; gene expression; genetically modified organisms; genome walking; ingredients; messenger RNA; models; pancreas; polysaccharides; prices; progeny; soybeans; sugars; swine; transgenes
Abstract:
... Competition between humans and livestock for cereal and legume grains makes it challenging to provide economical feeds to livestock animals. Recent increases in corn and soybean prices have had a significant impact on the cost of feed for pig producers. The utilization of byproducts and alternative ingredients in pig diets has the potential to reduce feed costs. Moreover, unlike ruminants, pigs ha ...
DOI:
10.1007/s11248-014-9817-9
PubMed:
25063310

45. Evaluating the Induced-Odour Emission of a Bt Maize and its Attractiveness to Parasitic Wasps

Author:
Turlings, T.C.J.; Jeanbourquin, P.M.; Held, M.; Degen, T.
Source:
Transgenic research 2005 v.14 no.6 pp. 807-816
ISSN:
0962-8819
Subject:
Zea mays; corn; transgenic plants; gene expression; transgenes; Bacillus thuringiensis; insecticidal proteins; odors; odor emissions; volatile organic compounds; Cotesia marginiventris; Microplitis rufiventris; parasitoids; natural enemies; insect attractants; insect behavior; tritrophic interactions
DOI:
10.1007/s11248-005-0008-6
PubMed:
16315088

46. Expression of a bacterial α-amylase gene in transgenic rice seeds

Author:
Xu, Xiaoli; Fang, Jun; Wang, Wei; Guo, Jianli; Chen, Pinnan; Cheng, Jiaan; Shen, Zhicheng
Source:
Transgenic research 2008 v.17 no.4 pp. 645-650
ISSN:
0962-8819
Subject:
rice; sugars; seeds; Geobacillus stearothermophilus; corn; starch; pH; genes; temperature; alpha-amylase
Abstract:
... An α-amylase gene from Bacillus stearothermophilus under the control of the promoter of a major rice-seed storage protein was introduced into rice. The transgenic line with the highest α-amylase activity reached about 15,000 U/g of seeds (one unit is defined as the amount of enzyme that produces 1 μmol of reducing sugar in 1 min at 70°C). The enzyme produced in the seeds had an optimum pH of 5.0-5 ...
DOI:
10.1007/s11248-007-9144-5

47. Expression of cold-tolerant pyruvate, orthophosphate dikinase cDNA, and heterotetramer formation in transgenic maize plants

Author:
Ohta, S.; Ishida, Y.; Usami, S.
Source:
Transgenic research 2004 v.13 no.5 pp. 475-485
ISSN:
0962-8819
Subject:
genetic transformation; Zea mays; complementary DNA; antisense DNA; transgenic plants; Agrobacterium radiobacter; corn; gene expression; protein quaternary structure; phosphotransferases (kinases); cold tolerance; Flaveria
DOI:
10.1007/s11248-004-1452-4
PubMed:
15587271

48. Expression of endogenous proteins in maize hybrids in a multi-location field trial in India

Author:
Linga R. Gutha; Divakar Purushottam; Aruna Veeramachaneni; Sarita Tigulla; Vikas Kodappully; Chandana Enjala; Hitendrasinh Rajput; Jennifer Anderson; Bonnie Hong; Jean Schmidt; Shveta Bagga
Source:
Transgenic research 2018 v.27 no.4 pp. 331-342
ISSN:
0962-8819
Subject:
actin; corn; crops; developmental stages; enzyme-linked immunosorbent assay; field experimentation; genotype; glyceraldehyde-3-phosphate dehydrogenase; growing season; hybrids; leaves; models; peptide elongation factors; plant tissues; safety assessment; transgenic plants; India
Abstract:
... Genetically modified (GM) crops undergo large scale multi-location field trials to characterize agronomics, composition, and the concentration of newly expressed protein(s) [herein referred to as transgenic protein(s)]. The concentration of transgenic proteins in different plant tissues and across the developmental stages of the plant is considered in the safety assessment of GM crops. Reference o ...
DOI:
10.1007/s11248-018-0077-y

49. Expression of the Newcastle disease virus fusion protein in transgenic maize and immunological studies

Author:
Octavio Guerrero-Andrade; Elizabeth Loza-Rubio; Teresa Olivera-Flores; Tamás Fehérvári-Bone; Miguel Angel Gómez-Lim
Source:
Transgenic research 2006 v.15 no.4 pp. 455-463
ISSN:
0962-8819
Subject:
chickens; viral fusion proteins; seeds; antigens; transgenes; antibodies; transgenic plants; vaccines; corn; Western blotting; gene expression; Avian orthoavulavirus 1; genetic engineering; ubiquitin; hormones
Abstract:
... Transgenic plants have been employed successfully as a low-cost system for the production of therapeutically valuable proteins, including antibodies, antigens and hormones. Here, we report the expression of the fusion (F) gene of the Newcastle disease virus (NDV) in transgenic maize plants. The expression of the transgene, driven by the maize ubiquitin promoter, caused accumulation of the F protei ...
DOI:
10.1007/s11248-006-0017-0

50. Expression of the lipid transfer protein Ace-AMP1 in transgenic wheat enhances antifungal activity and defense responses

Author:
Subhankar Roy-Barman; Christof Sautter; Bharat B. Chattoo
Source:
Transgenic research 2006 v.15 no.4 pp. 435
ISSN:
0962-8819
Subject:
Allium cepa; Blumeria graminis f. sp. tritici; Tilletia indica; Triticum aestivum; Western blotting; antifungal properties; antimicrobial proteins; chitinase; corn; disease resistance; fungi; genetic engineering; glufosinate; inheritance (genetics); introns; leaves; lipid transfer proteins; phenylalanine ammonia-lyase; polymerase chain reaction; salicylic acid; systemic acquired resistance; transgenes; transgenic plants; ubiquitin; wheat
Abstract:
... To enhance fungal disease resistance, wheat plants (cv. Bobwhite) were engineered to constitutively express the potent antimicrobial protein Ace-AMP1 from Allium cepa, driven by a maize ubiquitin promoter along with its first intron. The bar gene was used for selection of putative transformants on medium containing phosphinothricin (PPT). Transgene inheritance, integration and stability of express ...
DOI:
10.1007/s11248-006-0016-1

51. Functional analysis of two matrix attachment region (MAR) elements in transgenic maize plants

Author:
Sidorenko, L.; Bruce, W.; Maddock, S.; Tagliani, L.; Li, X.; Daniels, M.; Peterson, T.
Source:
Transgenic research 2003 v.12 no.2 pp. 137-154
ISSN:
0962-8819
Subject:
Zea mays; transgenes; transgenic plants; corn; nuclear matrix; gene expression; histochemistry; reporter genes; chromatin; promoter regions; beta-glucuronidase
PubMed:
12739882

52. Gene expression profiles of MON810 and comparable non-GM maize varieties cultured in the field are more similar than are those of conventional lines

Author:
Coll, Anna; Nadal, Anna; Collado, Rosa; Capellades, Gemma; Messeguer, Joaquima; Melé, Enric; Palaudelmàs, Montserrat; Pla, Maria
Source:
Transgenic research 2009 v.18 no.5 pp. 801-808
ISSN:
0962-8819
Subject:
corn; foods; gene expression; gene expression regulation; humans; leaves; microarray technology; transcriptome; transgenic plants
Abstract:
... Maize is a major food crop and genetically modified (GM) varieties represented 24% of the global production in 2007. Authorized GM organisms have been tested for human and environmental safety. We previously used microarrays to compare the transcriptome profiles of widely used commercial MON810 versus near-isogenic varieties and reported differential expression of a small set of sequences in leave ...
DOI:
10.1007/s11248-009-9266-z
PubMed:
19396622

53. Generation of marker-free transgenic maize by regular two-border Agrobacterium transformation vectors

Author:
Huang, S.; Gilbertson, L.A.; Adams, T.H.; Malloy, K.P.; Reisenbigler, E.K.; Birr, D.H.; Snyder, M.W.; Zhang, Q.; Luethy, M.H.
Source:
Transgenic research 2004 v.13 no.5 pp. 451-461
ISSN:
0962-8819
Subject:
genetic transformation; Zea mays; transgenic plants; Agrobacterium radiobacter; plasmid vectors; transfer DNA; corn; genetic markers
DOI:
10.1007/s11248-004-1453-3
PubMed:
15587269

54. Genetic engineering to improve essential and conditionally essential amino acids in maize: transporter engineering as a reference

Author:
Md. Mahmudul Hasan; Rima Rima
Source:
Transgenic research 2021 v.30 no.2 pp. 207-220
ISSN:
0962-8819
Subject:
biosynthesis; corn; food security; genes; genetic engineering
Abstract:
... Ruminants and humans are unable to synthesize essential amino acids (EAAs) and conditionally essential amino acids (CEAAs) under normal conditions and need to acquire them from plant sources. Maize plays, as a major crop, a central role in global food security. However, maize is deficient in several EAAs and CEAAs. Genetic engineering has been successfully used to enrich the EAA content of maize t ...
DOI:
10.1007/s11248-021-00235-0

55. Genetically modified crops and aquatic ecosystems: considerations for environmental risk assessment and non-target organism testing

Author:
Keri Carstens; Jennifer Anderson; Pamela Bachman; Adinda De Schrijver; Galen Dively; Brian Federici; Mick Hamer; Marco Gielkens; Peter Jensen; William Lamp; Stefan Rauschen; Geoff Ridley; Jörg Romeis; Annabel Waggoner
Source:
Transgenic research 2012 v.21 no.4 pp. 813-842
ISSN:
0962-8819
Subject:
Bacillus thuringiensis; agroecosystems; aquatic organisms; case studies; corn; crops; exposure assessment; exposure pathways; insecticidal proteins; models; toxicity testing; transgenic plants
Abstract:
... Environmental risk assessments (ERA) support regulatory decisions for the commercial cultivation of genetically modified (GM) crops. The ERA for terrestrial agroecosystems is well-developed, whereas guidance for ERA of GM crops in aquatic ecosystems is not as well-defined. The purpose of this document is to demonstrate how comprehensive problem formulation can be used to develop a conceptual model ...
DOI:
10.1007/s11248-011-9569-8
PubMed:
22120952
PubMed Central:
PMC3394238

56. Genetically modified maize impacts in Honduras: production and social issues

Author:
Diego Maximiliano Macall; Carlos Rogelio Trabanino; Alejandro Hernández Soto; Stuart J. Smyth
Source:
Transgenic research 2020 v.29 no.5-6 pp. 575-586
ISSN:
0962-8819
Subject:
biotechnology; corn; credit; federal government; food security; genetic engineering; interviews; pesticides; surveys; Honduras
Abstract:
... Maize production is one of the most important activities for the Honduran economy, both in terms of area cultivated and food security provided. This article reports the results of a survey undertaken to gauge knowledge, perceptions, opinions, and attitudes of Honduran farmers towards genetically modified (GM) maize. Data were collected from 32 maize producers in 2018–19, of both conventional and G ...
DOI:
10.1007/s11248-020-00221-y

57. Genome editing in cereal crops: an overview

Author:
Jerlie Mhay Matres; Julia Hilscher; Akash Datta; Victoria Armario-Nájera; Can Baysal; Wenshu He; Xin Huang; Changfu Zhu; Rana Valizadeh-Kamran; Kurniawan R. Trijatmiko; Teresa Capell; Paul Christou; Eva Stoger; Inez H. Slamet-Loedin
Source:
Transgenic research 2021 v.30 no.4 pp. 461-498
ISSN:
0962-8819
Subject:
barley; climate change; corn; gene editing; genome; grain quality; nutrient content; plant architecture; rice; wheat
Abstract:
... Genome-editing technologies offer unprecedented opportunities for crop improvement with superior precision and speed. This review presents an analysis of the current state of genome editing in the major cereal crops- rice, maize, wheat and barley. Genome editing has been used to achieve important agronomic and quality traits in cereals. These include adaptive traits to mitigate the effects of clim ...
DOI:
10.1007/s11248-021-00259-6

58. Heterologous expression of cellobiohydrolase II (Cel6A) in maize endosperm

Author:
Shivakumar Pattada Devaiah; Deborah Vicuna Requesens; Yeun-Kyung Chang; Kendall R. Hood; Ashley Flory; John A. Howard; Elizabeth E. Hood
Source:
Transgenic research 2013 v.22 no.3 pp. 477-488
ISSN:
0962-8819
Subject:
cellulose 1,4-beta-cellobiosidase; enzyme activity; corn; cellulose; endo-1,4-beta-glucanase; endosperm; microbial growth; biofuels; biotransformation; Trichoderma reesei; biomass
Abstract:
... The technology of converting lignocellulose to biofuels has advanced swiftly over the past few years, and enzymes are a significant constituent of this technology. In this regard, cost effective production of cellulases has been the focus of research for many years. One approach to reach cost targets of these enzymes involves the use of plants as bio-factories. The application of this technology t ...
DOI:
10.1007/s11248-012-9659-2
PubMed:
23080294

59. High level accumulation of α-glucan in maize kernels by expressing the gtfD gene from Streptococcus mutans

Author:
Zhang, Shirong; Dong, Jian G.; Wang, Tai; Guo, Sherry; Glassman, Kimberly; Ranch, Jerry; Nichols, Scott E.
Source:
Transgenic research 2007 v.16 no.4 pp. 467-478
ISSN:
0962-8819
Subject:
Streptococcus mutans; Zea mays; corn; crops; foods; glucose; glucosyltransferases; industrial applications; molecular weight; mutation; plant growth; polymerization; seeds; starch; sucrose; transgenes; transgenic plants
Abstract:
... Glucosyltransferases (GTFs, EC.2.4.1.5) are bacterial enzymes that catalyze the polymerization of glucose residues from sucrose, leading to the production of high molecular weight glucan with α-1,3 /α-1,6 linkages. Such glucans, with many potential food and industrial applications, do not normally exist in higher plants. We fused a mutant form of the gtfD gene from Sreptococcus mutans with the mai ...
DOI:
10.1007/s11248-006-9049-8

60. High-level expression of active human alpha1-antitrypsin in transgenic tobacco chloroplasts

Author:
Nadai, M.; Bally, J.; Vitel, M.; Job, C.; Tissot, G.; Botterman, J.; Dubald, M.
Source:
Transgenic research 2009 v.18 no.2 pp. 173-183
ISSN:
0962-8819
Subject:
chloroplasts; corn; elastase; humans; leaves; proteinase inhibitors; proteins; soybeans; terminator regions; tobacco
Abstract:
... We have produced human alpha1-antitrypsin (A1AT), a major therapeutic protein, in genetically engineered tobacco plastids. Four different expression vectors have been evaluated which encode A1AT under the control of various 5' and 3' plastid expression elements. The use of heterologous promoter and terminator sequences derived from the corn and soybean plastid genomes leads to simpler and predicta ...
DOI:
10.1007/s11248-008-9209-0
PubMed:
18686007

61. Impact of 9 years of Bt-maize cultivation on the distribution of maize viruses

Author:
Achon, Maria Angeles; Alonso-Dueñas, Natalia
Source:
Transgenic research 2009 v.18 no.3 pp. 387-397
ISSN:
0962-8819
Subject:
Bacillus thuringiensis; Maize dwarf mosaic virus; Maize rough dwarf virus; Sugarcane mosaic virus; corn; field experimentation; genetic background; viruses
Abstract:
... This study assesses the effect of Bt-maize on the distribution of maize viruses. Random surveys were conducted in Spain between 2001 and 2006 to evaluate the occurrence of maize viruses in Bt-maize cultivation areas and in areas where this crop had not been introduced. Maize dwarf mosaic virus (MDMV) was the predominant virus in Bt-areas, and Maize rough dwarf virus (MRDV) was the most predominant ...
DOI:
10.1007/s11248-008-9231-2
PubMed:
19067216

62. Impact of Bt-corn MON88017 in comparison to three conventional lines on Trigonotylus caelestialium (Kirkaldy) (Heteroptera: Miridae) field densities

Author:
Rauschen, Stefan; Schultheis, Eva; Pagel-Wieder, Sibylle; Schuphan, Ingolf; Eber, Sabine
Source:
Transgenic research 2009 v.18 no.2 pp. 203-214
ISSN:
0962-8819
Subject:
Diabrotica virgifera virgifera; Ostrinia nubilalis; Trigonotylus caelestialium; corn; enzyme-linked immunosorbent assay; field experimentation; nontarget organisms; transgenic plants
Abstract:
... In Europe, Bt-corn resistant against the European Corn Borer has until now been the only genetically modified plant to be grown commercially. With the advent of the Western Corn Rootworm Bt-corn varieties with resistance against Coleoptera will become important. The cultivation of Bt-plants may have negative impacts on non-target organisms, i.e. all species not explicitly targeted by a given Bt-cr ...
DOI:
10.1007/s11248-008-9207-2
PubMed:
18668336

63. Impact of gene stacking on gene flow: the case of maize

Author:
Lénaïc Paul; Frédérique Angevin; Cécile Collonnier; Antoine Messéan
Source:
Transgenic research 2012 v.21 no.2 pp. 243-256
ISSN:
0962-8819
Subject:
DNA; corn; foods; gene flow; genes; genetically modified organisms; models; pollen flow; quantitative polymerase chain reaction; seeds; stakeholders
Abstract:
... To respect the European labelling threshold for the adventitious presence of genetically modified organisms (GMOs) in food and feed, stakeholders mainly rely on real-time PCR analysis, which provides a measurement expressed as a percentage of GM-DNA. However, this measurement veils the complexity of gene flow, especially in the case of gene stacking. We have investigated the impact of gene stackin ...
DOI:
10.1007/s11248-011-9527-5
PubMed:
21681483

64. Impact of genetic structures on haploid genome-based quantification of genetically modified DNA: theoretical considerations, experimental data in MON 810 maize kernels (Zea mays L.) and some practical applications

Author:
David Zhang; Aurélie Corlet; Stephane Fouilloux
Source:
Transgenic research 2008 v.17 no.3 pp. 393-402
ISSN:
0962-8819
Subject:
quantitative polymerase chain reaction; stakeholders; crop production; Zea mays; genetically modified organisms; haploidy; hybrids; loci; monitoring; equations; DNA; genome; males; corn; models; endosperm; homozygosity; females; prediction
Abstract:
... Real-time Polymerase Chain Reaction (PCR) based assays are widely used to estimate the content of genetically modified (GM) materials in food, feed and seed. It has been known that the genetic structures of the analyte can significantly influence the GM content expressed by the haploid genome (HG) % estimated using real-time PCR assays; this kind of influence is also understood as the impact of bi ...
DOI:
10.1007/s11248-007-9114-y

65. Influence of calcareous soil on Cry3Bb1 expression and efficacy in the field

Author:
David S. Wangila; Arnubio Valencia J; Haichuan Wang; Blair D. Siegfried; Lance J. Meinke
Source:
Transgenic research 2017 v.26 no.3 pp. 419-428
ISSN:
0962-8819
Subject:
Diabrotica virgifera virgifera; Zea mays; calcareous soils; chlorosis; corn; crystal proteins; developmental stages; edaphic factors; eggs; enzyme-linked immunosorbent assay; genetic engineering; hybrids; insecticidal proteins; iron; protein synthesis; quantitative analysis; roots; soil pH; tissues; Nebraska
Abstract:
... Greater than expected injury by western corn rootworm (WCR) (Diabrotica virgifera virgifera LeConte) to Cry3Bb1 expressing maize hybrids (Zea mays L.) has been reported in southwestern Nebraska. Affected areas of some fields are often associated with high pH calcareous soils where maize growth is poor and iron chlorosis is common. As part of a comprehensive study to understand potential causes of ...
DOI:
10.1007/s11248-017-0014-5

66. Ingestion and excretion of two transgenic Bt corn varieties by slugs

Author:
Zurbrügg, Corinne; Nentwig, Wolfgang
Source:
Transgenic research 2009 v.18 no.2 pp. 215-225
ISSN:
0962-8819
Subject:
Arion; Bacillus thuringiensis; Deroceras reticulatum; arthropods; beneficial organisms; corn; endotoxins; enzyme-linked immunosorbent assay; excretion; feces; food webs; ingestion; intestines; leaves; pests; predators; protein content; proteins; risk assessment; slugs; transgenic plants
Abstract:
... The release of transgenic Bacillus thuringiensis (Bt) corn expressing various Cry endotoxins has raised concern that these endotoxins are disseminated in the food web and may adversely affect non-target beneficial organisms, such as predators and organisms of the decomposer food web. We therefore investigated in a laboratory study, whether the Cry1Ab and Cry3Bb1 protein from Bt corn could potentia ...
DOI:
10.1007/s11248-008-9208-1
PubMed:
18763046

67. Laboratory toxicity studies demonstrate no adverse effects of Cry1Ab and Cry3Bb1 to larvae of Adalia bipunctata (Coleoptera: Coccinellidae): the importance of study design

Author:
Álvarez-Alfageme, Fernando; Bigler, Franz; Romeis, Jörg
Source:
Transgenic research 2011 v.20 no.3 pp. 467-479
ISSN:
0962-8819
Subject:
Adalia bipunctata; Bacillus thuringiensis; Tetranychidae; adverse effects; corn; experimental design; exposure pathways; ingestion; larvae; mortality; proteins; sucrose; toxicity; toxicity testing; transgenic plants
Abstract:
... Scientific studies are frequently used to support policy decisions related to transgenic crops. Schmidt et al., Arch Environ Contam Toxicol 56:221-228 (2009) recently reported that Cry1Ab and Cry3Bb were toxic to larvae of Adalia bipunctata in direct feeding studies. This study was quoted, among others, to justify the ban of Bt maize (MON 810) in Germany. The study has subsequently been criticized ...
DOI:
10.1007/s11248-010-9430-5
PubMed:
20740377
PubMed Central:
PMC3090567

68. Maize hybrids derived from GM positive and negative segregant inbreds are compositionally equivalent: any observed differences are associated with conventional backcrossing practices

Author:
Tyamagondlu V. Venkatesh; Erin Bell; Anna Bickel; Kevin Cook; Benjamin Alsop; Martijn van de Mortel; Ping Feng; Alan Willse; Tim Perez; Susan Riordan; George G. Harrigan
Source:
Transgenic research 2016 v.25 no.1 pp. 83-96
ISSN:
0962-8819
Subject:
amino acids; backcrossing; beta-carotene; corn; fatty acids; females; herbicide resistance; hybrids; males; minerals; oils; phytic acid; raffinose; starch; statistical analysis; tocopherols; transgenic plants; variance; United States
Abstract:
... In this study, we show that compositional differences in grain harvested from genetically modified (GM) maize hybrids derived from near-isogenic trait-positive and trait-negative segregant inbreds are more likely related to backcrossing practices than to the GM trait. To demonstrate this, four paired GM trait-positive (NK603: herbicide tolerance) and trait-negative near-isogenic inbred male lines ...
DOI:
10.1007/s11248-015-9910-8

69. Meeting technical challenges for protein characterization and surrogate equivalence studies that resulted from insecticidal protein co-expression in maize event MZIR098

Author:
Frederick S. Walters; Scott Young; Gerson Graser
Source:
Transgenic research 2020 v.29 no.1 pp. 109-124
ISSN:
0962-8819
Subject:
bioactive properties; corn; genetic engineering; immunogenicity; insecticidal proteins; molecular weight; pests; plant proteins; protein sources; protein synthesis; safety assessment
Abstract:
... Safety assessment of genetically modified plants includes protein characterization to confirm the intended trait protein expression. In addition, to conduct safety tests, the large amount of purified protein needed is usually met through the use of a surrogate, microbially produced protein source. Characterization of the eCry3.1Ab and mCry3A proteins as derived from Event MZIR098 maize was challen ...
DOI:
10.1007/s11248-019-00183-w
PubMed:
31781961
PubMed Central:
PMC7000486

70. Metabolic engineering of astaxanthin biosynthesis in maize endosperm and characterization of a prototype high oil hybrid

Author:
Gemma Farré; Laura Perez-Fons; Mathilde Decourcelle; Jürgen Breitenbach; Sonia Hem; Changfu Zhu; Teresa Capell; Paul Christou; Paul D. Fraser; Gerhard Sandmann
Source:
Transgenic research 2016 v.25 no.4 pp. 477-489
ISSN:
0962-8819
Subject:
genetic background; corn oil; genes; genetic engineering; genetically modified organisms; hybrids; parents; lycopene; beta-carotene; amino acids; tricarboxylic acid cycle; gene expression regulation; metabolomics; glyceraldehyde 3-phosphate; Zea mays; pyruvic acid; principal component analysis; corn; lycopene epsilon-cyclase; proteomics; biosynthesis; astaxanthin; metabolic engineering; endosperm; phytoene synthase
Abstract:
... Maize was genetically engineered for the biosynthesis of the high value carotenoid astaxanthin in the kernel endosperm. Introduction of a β-carotene hydroxylase and a β-carotene ketolase into a white maize genetic background extended the carotenoid pathway to astaxanthin. Simultaneously, phytoene synthase, the controlling enzyme of carotenogenesis, was over-expressed for enhanced carotenoid produc ...
DOI:
10.1007/s11248-016-9943-7

71. Multigene engineering of starch biosynthesis in maize endosperm increases the total starch content and the proportion of amylose

Author:
Lili Jiang; Xiaoming Yu; Xin Qi; Qian Yu; Sen Deng; Bing Bai; Ning Li; Ai Zhang; Changfu Zhu; Bao Liu; Jinsong Pang
Source:
Transgenic research 2013 v.22 no.6 pp. 1133-1142
ISSN:
0962-8819
Subject:
modified starch; engineering; RNA interference; Zea mays; biosynthesis; amylose; 1,4-alpha-glucan branching enzyme; corn; agronomic traits; endosperm; sucrose synthase; starch synthase
Abstract:
... Maize (Zea mays spp. mays) is a staple crop for more than 900 million people. The seeds or kernels provide a rich source of calories because ~70 % of the weight is carbohydrate, mostly in the form of starch. The content and composition of starch are complex traits controlled by many genes, offering multiple potential targets for intervention. We used a multigene engineering approach combining the ...
DOI:
10.1007/s11248-013-9717-4
PubMed:
23740205

72. Mycotoxin Reduction in Bt Corn: Potential Economic, Health, and Regulatory Impacts

Author:
Wu, F.
Source:
Transgenic research 2006 v.15 no.3 pp. 277-289
ISSN:
0962-8819
Subject:
imports; crystal proteins; plant pathogenic fungi; pest resistance; insecticidal proteins; genetic resistance; fungal diseases of plants; gene expression; Bacillus thuringiensis; international trade; disease resistance; economic impact; Zea mays; corn; aflatoxins; fumonisins; human food chain; transgenes; transgenic plants; food contamination
DOI:
10.1007/s11248-005-5237-1
PubMed:
16779644

73. Next-generation sequencing is a robust strategy for the high-throughput detection of zygosity in transgenic maize

Author:
Leonie Fritsch; Rainer Fischer; Christoph Wambach; Max Dudek; Stefan Schillberg; Florian Schröper
Source:
Transgenic research 2015 v.24 no.4 pp. 615-623
ISSN:
0962-8819
Subject:
DNA; alleles; biotechnology; business enterprises; corn; genotyping; high-throughput nucleotide sequencing; loci; monitoring; nucleotide sequences; plant breeding; quantitative polymerase chain reaction; transgenes; transgenic plants
Abstract:
... Simple and reliable, high-throughput techniques to detect the zygosity of transgenic events in plants are valuable for biotechnology and plant breeding companies seeking robust genotyping data for the assessment of new lines and the monitoring of breeding programs. We show that next-generation sequencing (NGS) applied to short PCR products spanning the transgene integration site provides accurate ...
DOI:
10.1007/s11248-015-9864-x

74. No effects of Bacillus thuringiensis maize on nontarget organisms in the field in southern Europe: a meta-analysis of 26 arthropod taxa

Author:
C. Comas; B. Lumbierres; X. Pons; R. Albajes
Source:
Transgenic research 2014 v.23 no.1 pp. 135-143
ISSN:
0962-8819
Subject:
Bacillus thuringiensis; arthropods; corn; entomopathogenic bacteria; field experimentation; insecticidal properties; meta-analysis; nontarget organisms; pitfall traps; statistical analysis; sticky traps; transgenic plants
Abstract:
... Maize with the insecticidal properties of the entomopathogenic bacterium Bacillus thuringiensis Berliner, known as Bt maize, has been sown in Europe since 1998. For several years, EU and Spanish regulations have required laboratory and field trials to assess risks of genetically modified crops for nontarget organisms prior to their authorization. Thirteen field trials were conducted in Spain to me ...
DOI:
10.1007/s11248-013-9737-0
PubMed:
23904218

75. Non-target organism effects tests on Vip3A and their application to the ecological risk assessment for cultivation of MIR162 maize

Author:
Raybould, Alan; Vlachos, Demetra
Source:
Transgenic research 2011 v.20 no.3 pp. 599-611
ISSN:
0962-8819
Subject:
Bacillus thuringiensis; Daphnia magna; Lepidoptera; adverse effects; biological control; corn; crop yield; crops; evolution; insecticidal proteins; mechanism of action; nontarget organisms; pest resistance; pesticide application; pests; risk assessment; transgenic plants
Abstract:
... Transgenic crops producing insecticidal proteins from Bacillus thuringiensis (Bt) provide economic, environmental and health benefits by maintaining or increasing crop yields with fewer applications of insecticide. To sustain these benefits, it is important to delay the evolution of insect resistance to the proteins, and to ensure that the proteins do not harm non-target organisms, particularly th ...
DOI:
10.1007/s11248-010-9442-1
PubMed:
20839052

76. Nontarget organism effects tests on eCry3.1Ab and their application to the ecological risk assessment for cultivation of Event 5307 maize

Author:
Andrea Burns; Alan Raybould
Source:
Transgenic research 2014 v.23 no.6 pp. 985-994
ISSN:
0962-8819
Subject:
Diabrotica virgifera virgifera; corn; diet; environmental exposure; genetically modified organisms; laboratory techniques; nontarget organisms; pests; plant tissues; risk; risk assessment
Abstract:
... Event 5307 transgenic maize produces the novel insecticidal protein eCry3.1Ab, which is active against certain coleopteran pests such as Western corn rootworm (Diabrotica virgifera virgifera). Laboratory tests with representative nontarget organisms (NTOs) were conducted to test the hypothesis of no adverse ecological effects of cultivating Event 5307 maize. Estimates of environmental eCry3.1Ab co ...
DOI:
10.1007/s11248-013-9778-4
PubMed:
24407432
PubMed Central:
PMC4204007

77. Occurrence and field densities of Coleoptera in the maize herb layer: implications for Environmental Risk Assessment of genetically modified Bt-maize

Author:
Rauschen, Stefan; Schaarschmidt, Frank; Gathmann, Achim
Source:
Transgenic research 2010 v.19 no.5 pp. 727-744
ISSN:
0962-8819
Subject:
Bacillus thuringiensis; Chrysomelidae; Coccinellidae; Lepidoptera; biological control agents; corn; environmental assessment; field experimentation; growing season; insects; laboratory experimentation; nontarget organisms; pests; risk assessment; statistical analysis; transgenic plants
Abstract:
... Beetles (Coleoptera) are a diverse and ecologically important group of insects in agricultural systems. The Environmental Risk Assessment (ERA) of genetically modified Bt-crop varieties with insect resistances thus needs to consider and assess the potential negative impacts on non-target organisms belonging to this group. We analysed data gathered during 6 years of field-release experiments on the ...
DOI:
10.1007/s11248-009-9351-3
PubMed:
20012775

78. Oral immunogenicity and protective efficacy in mice of transgenic rice plants producing a vaccine candidate antigen (As16) of Ascaris suum fused with cholera toxin B subunit

Author:
Matsumoto, Yasunobu; Suzuki, Seiko; Nozoye, Tomoko; Yamakawa, Takashi; Takashima, Yasuhiro; Arakawa, Takeshi; Tsuji, Naotoshi; Takaiwa, Fumio; Hayashi, Yoshihiro
Source:
Transgenic research 2009 v.18 no.2 pp. 185-192
ISSN:
0962-8819
Subject:
rice; adjuvants; grain crops; blood serum; antigens; oral administration; Oryza sativa; antibodies; recombinant fusion proteins; Ascaris suum; corn; secondary immunization; transgenic plants; mice; vaccines; endosperm; immune response; cholera toxin; eggs
Abstract:
... Cereal crops such as maize and rice are considered attractive for vaccine production and oral delivery. Here, we evaluated the rice Oryza sativa for production of As16--an antigen protective against the roundworm Ascaris suum. The antigen was produced as a chimeric protein fused with cholera toxin B subunit (CTB), and its expression level in the endosperm reached 50 μg/g seed. Feeding the transgen ...
DOI:
10.1007/s11248-008-9205-4
PubMed:
18763047

79. Over-expression of the cucumber expansin gene (Cs-EXPA1) in transgenic maize seed for cellulose deconstruction

Author:
Sangwoong Yoon; Shivakumar P. Devaiah; Seo-eun Choi; Jeff Bray; Robert Love; Jeffrey Lane; Carol Drees; John H. Howard; Elizabeth E. Hood
Source:
Transgenic research 2016 v.25 no.2 pp. 173-186
ISSN:
0962-8819
Subject:
biofuels; biomass; breeding; cell walls; cellulose; corn; cucumbers; endo-1,4-beta-glucanase; endoplasmic reticulum; fuel production; gene overexpression; genes; growth and development; lignocellulose; seeds; sugars; synergism; transgenic plants; vacuoles
Abstract:
... Plant cell wall degradation into fermentable sugars by cellulases is one of the greatest barriers to biofuel production. Expansin protein loosens the plant cell wall by opening up the complex of cellulose microfibrils and polysaccharide matrix components thereby increasing its accessibility to cellulases. We over-expressed cucumber expansin in maize kernels to produce enough protein to assess its ...
DOI:
10.1007/s11248-015-9925-1

80. Overexpression of a glutamine synthetase gene affects growth and development in sorghum

Author:
Jazmina Urriola; Keerti S. Rathore
Source:
Transgenic research 2015 v.24 no.3 pp. 397-407
ISSN:
0962-8819
Subject:
Agrobacterium; Sorghum bicolor; Zea mays; adenosine triphosphate; ammonia; biomass; corn; crops; environmental factors; enzyme activity; gene overexpression; genes; genetic transformation; glutamate-ammonia ligase; glutamic acid; glutamine; grain yield; growth and development; messenger RNA; nitrogen; nitrogen metabolism; nutrient use efficiency; plant growth; progeny; promoter regions; shoots; tillering; transgenic plants; ubiquitin
Abstract:
... Nitrogen is a primary macronutrient in plants, and nitrogen fertilizers play a critical role in crop production and yield. In this study, we investigated the effects of overexpressing a glutamine synthetase (GS) gene on nitrogen metabolism, and plant growth and development in sorghum (Sorghum bicolor L., Moench). GS catalyzes the ATP dependent reaction between ammonia and glutamate to produce glut ...
DOI:
10.1007/s11248-014-9852-6

81. Overexpression of the maize γ-tocopherol methyltransferase gene (ZmTMT) increases α-tocopherol content in transgenic Arabidopsis and maize seeds

Author:
Lan Zhang; Yanzhong Luo; Bin Liu; Liang Zhang; Wei Zhang; Rumei Chen; Lei Wang
Source:
Transgenic research 2020 v.29 no.1 pp. 95-104
ISSN:
0962-8819
Subject:
Arabidopsis thaliana; Escherichia coli; Glycine max; Zea mays; alpha-tocopherol; antioxidants; biochemical pathways; corn; feed industry; genes; genetic engineering; humans; livestock; tocotrienols
Abstract:
... The vitamin E family includes tocopherols and tocotrienols, which are essential lipid-soluble antioxidants necessary for human and livestock health. The seeds of many plant species, including maize, have high gamma (γ)-tocopherol but low alpha (α)-tocopherol contents; however, α-tocopherol is the most effective antioxidant. Therefore, it is necessary to optimize the tocopherol composition in plant ...
DOI:
10.1007/s11248-019-00180-z

82. Plant characterization of genetically modified maize hybrids MON-89Ø34-3 × MON-88Ø17-3, MON-89Ø34-3 × MON-ØØ6Ø3-6, and MON-ØØ6Ø3-6: alternatives for maize production in Mexico

Author:
Oscar Heredia Díaz; José Luis Aldaba Meza; Baltazar M. Baltazar; Germán Bojórquez Bojórquez; Luciano Castro Espinoza; José Luis Corrales Madrid; Juan Manuel de la Fuente Martínez; Héctor Abel Durán Pompa; José Alonso Escobedo; Armando Espinoza Banda; José Antonio Garzón Tiznado; Juvencio González García; José Luis Guzmán Rodríguez; Jesús Ignacio Madueño Martínez; José Luis Martínez Carrillo; Chen Meng; Francisco Javier Quiñones Pando; Enrique Rosales Robles; Ignacio Ruiz Hernández; José Elías Treviño Ramírez; Hugo Raúl Uribe Montes; Francisco Zavala García
Source:
Transgenic research 2017 v.26 no.1 pp. 135-151
ISSN:
0962-8819
Subject:
biotechnology; corn; corn ears; cost effectiveness; crops; ecoregions; environmental assessment; farmers; genetic background; genetic engineering; grain yield; herbicide resistance; hybrids; insect pests; lodging; meta-analysis; phenotype; risk; risk assessment; seedlings; transgenic plants; vigor; weed control; Mexico
Abstract:
... Environmental risk assessment (ERA) of genetically modified (GM) crops is a process to evaluate whether the biotechnology trait(s) in a GM crop may result in increased pest potential or harm to the environment. In this analysis, two GM insect-resistant (IR) herbicide-tolerant maize hybrids (MON-89Ø34-3 × MON-88Ø17-3 and MON-89Ø34-3 × MON-ØØ6Ø3-6) and one herbicide-tolerant GM hybrid (MON-ØØ6Ø3-6) ...
DOI:
10.1007/s11248-016-9991-z
PubMed:
27771867
PubMed Central:
PMC5243880

83. Plant development inhibitory genes in binary vector backbone improve quality event efficiency in soybean transformation

Author:
Xudong Ye; Edward J. Williams; Junjiang Shen; James A. Esser; Amy M. Nichols; Michael W. Petersen; Larry A. Gilbertson
Source:
Transgenic research 2008 v.17 no.5 pp. 827-838
ISSN:
0962-8819
Subject:
Phaseolus; corn; gene expression; levansucrase; phytoene synthase; plant development; soybeans; transgenes; transgenic plants; vector control
Abstract:
... Conventional Agrobacterium-mediated plant transformation often produces a significant frequency of transgenic events containing vector backbone sequence, which is generally undesirable for biotechnology applications. We tested methods to reduce the frequency of transgenic plants containing vector backbone by incorporating genes into the backbone that inhibit the development of transgenic plants. F ...
DOI:
10.1007/s11248-008-9169-4
PubMed:
18253857

84. Plurality of opinion, scientific discourse and pseudoscience: an in depth analysis of the Séralini et al. study claiming that Roundup™ Ready corn or the herbicide Roundup™ cause cancer in rats

Author:
Gemma Arjó; Manuel Portero; Carme Piñol; Juan Viñas; Xavier Matias-Guiu; Teresa Capell; Andrew Bartholomaeus; Wayne Parrott; Paul Christou
Source:
Transgenic research 2013 v.22 no.2 pp. 255-267
ISSN:
0962-8819
Subject:
corn; herbicides; laboratory animals; rats; toxicity
Abstract:
... A recent paper published in the journal Food and Chemical Toxicology presents the results of a long-term toxicity study related to a widely-used commercial herbicide (Roundup™) and a Roundup-tolerant genetically modified variety of maize, concluding that both the herbicide and the maize varieties are toxic. Here we discuss the many errors and inaccuracies in the published article resulting in high ...
DOI:
10.1007/s11248-013-9692-9
PubMed:
23430588

85. Potential subchronic food safety of the stacked trait transgenic maize GH5112E-117C in Sprague-Dawley rats

Author:
Shiwen Han; Shiying Zou; Xiaoyun He; Kunlun Huang; Xiaohong Mei
Source:
Transgenic research 2016 v.25 no.4 pp. 453-463
ISSN:
0962-8819
Subject:
body weight; chemistry; corn; food consumption; food safety; genes; genetic engineering; glyphosate; hematology; herbicide resistance; histopathology; rats; transgenic plants
Abstract:
... The food safety of stacked trait genetically modified (GM) maize GH5112E-117C containing insect-resistance gene Cry1Ah and glyphosate-resistant gene G2-aroA was evaluated in comparison to non-GM Hi-II maize fed to Sprague-Dawley rats during a 90-day subchronic feeding study. Three different dietary concentrations (12.5, 25 and 50 %, w/w) of the GM maize were used or its corresponding non-GM maize. ...
DOI:
10.1007/s11248-016-9944-6

86. Potential use of an arthropod database to support the non-target risk assessment and monitoring of transgenic plants

Author:
Jörg Romeis; Michael Meissle; Fernando Álvarez-Alfageme; Franz Bigler; David A. Bohan; Yann Devos; Louise A. Malone; Xavier Pons; Stefan Rauschen
Source:
Transgenic research 2014 v.23 no.6 pp. 995-1013
ISSN:
0962-8819
Subject:
agroecosystems; arthropods; case studies; corn; databases; ecosystem services; environmental monitoring; genetic engineering; risk; risk assessment; transgenic plants; Europe
Abstract:
... Worldwide, plants obtained through genetic modification are subject to a risk analysis and regulatory approval before they can enter the market. An area of concern addressed in environmental risk assessments is the potential of genetically modified (GM) plants to adversely affect non-target arthropods and the valued ecosystem services they provide. Environmental risk assessments are conducted case ...
DOI:
10.1007/s11248-014-9791-2
PubMed:
24633599

87. Prey mediated effects of Bt maize on fitness and digestive physiology of the red spider mite predator Stethorus punctillum Weise (Coleoptera: Coccinellidae)

Author:
Fernando Álvarez-Alfageme; Natalie Ferry; Pedro Castañera; Felix Ortego; Angharad M. R. Gatehouse
Source:
Transgenic research 2008 v.17 no.5 pp. 943-954
ISSN:
0962-8819
Subject:
Bacillus thuringiensis; Stethorus punctillum; Tetranychus urticae; adulthood; cattle; corn; cysteine proteinases; digestion; fecundity; in vitro studies; insecticidal properties; trophic relationships; trypsin
Abstract:
... The present study investigated prey-mediated effects of two maize varieties expressing a truncated Cry1Ab, Compa CB (event Bt176) and DKC7565 (event MON810), on the biology of the ladybird Stethorus punctillum. Although immuno-assays demonstrated the presence of Cry1Ab in both prey and predator collected from commercial maize-growing fields, neither transgenic variety had any negative effects on s ...
DOI:
10.1007/s11248-008-9177-4
PubMed:
18322817

88. Provitamin A carotenoids from an engineered high-carotenoid maize are bioavailable and zeaxanthin does not compromise β-carotene absorption in poultry

Author:
Joana Díaz-Gómez; Jose A. Moreno; Eduardo Angulo; Gerhard Sandmann; Changfu Zhu; Teresa Capell; Carmina Nogareda
Source:
Transgenic research 2017 v.26 no.5 pp. 591-601
ISSN:
0962-8819
Subject:
absorption; artificial colors; beta-carotene; beta-cryptoxanthin; bioavailability; biofortification; chickens; corn; diet; genetic engineering; intestines; liver; lutein; poultry feeding; poultry industry; vitamin A; zeaxanthin
Abstract:
... High-carotenoid (HC) maize, a biofortified staple crop which accumulates β-carotene, β-cryptoxanthin, lutein and zeaxanthin, was used as a feed component in a chicken feeding trial to assess the bioavailability of provitamin A (PVA) carotenoids in the kernel matrix compared to the synthetic and natural color additives routinely used in the poultry industry. We found that the PVA carotenoids in HC ...
DOI:
10.1007/s11248-017-0029-y

89. Quality of laboratory studies assessing effects of Bt-proteins on non-target organisms: minimal criteria for acceptability

Author:
Adinda De Schrijver; Yann Devos; Patrick De Clercq; Achim Gathmann; Jörg Romeis
Source:
Transgenic research 2016 v.25 no.4 pp. 395-411
ISSN:
0962-8819
Subject:
Annelida; Arthropoda; Bacillus thuringiensis; bacterial proteins; corn; ecosystem services; genetic engineering; nontarget organisms; risk; risk assessment; toxins; transgenic plants
Abstract:
... The potential risks that genetically modified plants may pose to non-target organisms and the ecosystem services they contribute to are assessed as part of pre-market risk assessments. This paper reviews the early tier studies testing the hypothesis whether exposure to plant-produced Cry34/35Ab1 proteins as a result of cultivation of maize 59122 is harmful to valued non-target organisms, in partic ...
DOI:
10.1007/s11248-016-9950-8

90. Recombinant protein expression plasmids optimized for industrial E. coli fermentation and plant systems produce biologically active human insulin-like growth factor-1 in transgenic rice and tobacco plants

Author:
Panahi, M.; Alli, Z.; Cheng, X.; Belbaraka, L.; Belgoudi, J.; Sardana, R.; Phipps, J.; Altosaar, I.
Source:
Transgenic research 2004 v.13 no.3 pp. 245-259
ISSN:
0962-8819
Subject:
Oryza sativa; rice; Nicotiana tabacum; tobacco; transgenic plants; Escherichia coli; genetically engineered microorganisms; genetic transformation; plasmid vectors; transgenes; insulin-like growth factor I; humans; gene expression; recombinant proteins; genetic code; promoter regions; ubiquitin; Zea mays; corn; cell proliferation; neurons; cell lines
DOI:
10.1023/B:TRAG.0000034619.21613.d0
PubMed:
15359602

91. Reconstruction of the astaxanthin biosynthesis pathway in rice endosperm reveals a metabolic bottleneck at the level of endogenous β-carotene hydroxylase activity

Author:
Chao Bai; Judit Berman; Gemma Farre; Teresa Capell; Gerhard Sandmann; Paul Christou; Changfu Zhu
Source:
Transgenic research 2017 v.26 no.1 pp. 13-23
ISSN:
0962-8819
Subject:
Chlamydomonas reinhardtii; Oryza sativa; Pantoea ananatis; astaxanthin; beta-carotene; biosynthesis; canthaxanthin; corn; endosperm; genetic engineering; hydroxylation; phytoene synthase; rice; tissues; transgenic plants
Abstract:
... Astaxanthin is a high-value ketocarotenoid rarely found in plants. It is derived from β-carotene by the 3-hydroxylation and 4-ketolation of both ionone end groups, in reactions catalyzed by β-carotene hydroxylase and β-carotene ketolase, respectively. We investigated the feasibility of introducing an extended carotenoid biosynthesis pathway into rice endosperm to achieve the production of astaxant ...
DOI:
10.1007/s11248-016-9977-x

92. Resistance evolution to the first generation of genetically modified Diabrotica-active Bt-maize events by western corn rootworm: management and monitoring considerations

Author:
Yann Devos; Lisa N. Meihls; József Kiss; Bruce E. Hibbard
Source:
Transgenic research 2013 v.22 no.2 pp. 269-299
ISSN:
0962-8819
Subject:
Diabrotica virgifera virgifera; alleles; artificial selection; corn; evolution; farm income; insects; integrated pest management; larvae; monitoring; pests; resistance management; risk management; roots; uncertainty; United States
Abstract:
... Western corn rootworm (Diabrotica virgifera virgifera; WCR) is a major coleopteran maize pest in North America and the EU, and has traditionally been managed through crop rotation and broad-spectrum soil insecticides. Genetically modified Bt-maize offers an additional management tool for WCR and has been valuable in reducing insecticide use and increasing farm income. A concern is that the widespr ...
DOI:
10.1007/s11248-012-9657-4
PubMed:
23011587

93. Safety of Pseudomonas chlororaphis as a gene source for genetically modified crops

Author:
Jennifer A. Anderson; Jamie Staley; Mary Challender; Jamie Heuton
Source:
Transgenic research 2018 v.27 no.1 pp. 103-113
ISSN:
0962-8819
Subject:
Coleoptera; Pseudomonas chlororaphis; Zea mays; allergenicity; corn; crops; genes; genetic engineering; genetically modified plants; insecticidal properties; pests; safety assessment; toxicity
Abstract:
... Genetically modified crops undergo extensive evaluation to characterize their food, feed and environmental safety prior to commercial introduction, using a well-established, science-based assessment framework. One component of the safety assessment includes an evaluation of each introduced trait, including its source organism, for potential adverse pathogenic, toxic and allergenic effects. Several ...
DOI:
10.1007/s11248-018-0061-6
PubMed:
29427161
PubMed Central:
PMC5847145

94. Simultaneous expression of Arabidopsis ρ-hydroxyphenylpyruvate dioxygenase and MPBQ methyltransferase in transgenic corn kernels triples the tocopherol content

Author:
Naqvi, Shaista; Farré, Gemma; Zhu, Changfu; Sandmann, Gerhard; Capell, Teresa; Christou, Paul
Source:
Transgenic research 2011 v.20 no.1 pp. 177-181
ISSN:
0962-8819
Subject:
Arabidopsis; biochemical pathways; biofortification; clones; complementary DNA; corn; gamma-tocopherol; gene expression; human health; isomers; metabolic engineering; methyltransferases; seeds; substrate specificity; transgenic plants
Abstract:
... The quantity and composition of tocopherols (compounds with vitamin E activity) vary widely among different plant species reflecting the expression, activity and substrate specificity of enzymes in the corresponding metabolic pathway. Two Arabidopsis cDNA clones corresponding to ρ-hydroxyphenylpyruvate dioxygenase (HPPD) and 2-methyl-6-phytylplastoquinol methyltransferase (MPBQ MT) were constituti ...
DOI:
10.1007/s11248-010-9393-6

95. Six Years after the Commercial Introduction of Bt Maize in Spain: Field Evaluation, Impact and Future Prospects

Author:
Eizaguirre, M.; Albajes, R.; Lopez, C.; Eras, J.; Lumbierres, B.; Pons, X.
Source:
Transgenic research 2006 v.15 no.1 pp. 1-12
ISSN:
0962-8819
Subject:
Zea mays; corn; transgenic plants; transgenes; Bacillus thuringiensis; insecticidal proteins; crystal proteins; gene expression; insecticide resistance; Sesamia nonagrioides; insect pests; defense mechanisms; larvae; dispersal behavior; field experimentation; nontarget organisms; environmental impact; predatory insects; Spain
DOI:
10.1007/s11248-005-3998-1
PubMed:
16475005

96. Strategies to improve low copy transgenic events in Agrobacterium-mediated transformation of maize

Author:
Elumalai Sivamani; Xianggan Li; Samson Nalapalli; Yoshimi Barron; Anna Prairie; David Bradley; Michele Doyle; Qiudeng Que
Source:
Transgenic research 2015 v.24 no.6 pp. 1017-1027
ISSN:
0962-8819
Subject:
3-phosphoshikimate 1-carboxyvinyltransferase; RNA interference; corn; genetic markers; mannose-6-phosphate isomerase; transcription factors; transfer DNA; transgenes; transgenic plants
Abstract:
... Transgenic plants containing low copy transgene insertion free of vector backbone are highly desired for many biotechnological applications. We have investigated two different strategies for increasing the percentage of low copy events in Agrobacterium-mediated transformation experiments in maize. One of the strategies is to use a binary vector with two separate T-DNAs, one T-DNA containing an int ...
DOI:
10.1007/s11248-015-9902-8

97. Thanatin confers partial resistance against aflatoxigenic fungi in maize (Zea mays)

Author:
Max Schubert; Marcel Houdelet; Karl-Heinz Kogel; Rainer Fischer; Stefan Schillberg; Greta Nölke
Source:
Transgenic research 2015 v.24 no.5 pp. 885-895
ISSN:
0962-8819
Subject:
Aspergillus flavus; Podisus maculiventris; Zea mays; aflatoxins; animal and human health; anti-infective properties; antimicrobial peptides; apoplast; complementary DNA; corn; crops; fungi; heterologous gene expression; in vitro studies; minimum inhibitory concentration; reverse transcriptase polymerase chain reaction; risk; secondary metabolites; seeds; storage conditions; transgenic plants
Abstract:
... Aflatoxin-producing fungi can contaminate plants and plant-derived products with carcinogenic secondary metabolites that present a risk to human and animal health. In this study, we investigated the effect of antimicrobial peptides on the major aflatoxigenic fungi Aspergillus flavus and A. parasiticus. In vitro assays with different chemically-synthesized peptides demonstrated that the broad-spect ...
DOI:
10.1007/s11248-015-9888-2

98. The Romanian experience and perspective on the commercial cultivation of genetically modified crops in Europe

Author:
Mihael Cristin Ichim
Source:
Transgenic research 2019 v.28 no.1 pp. 1-7
ISSN:
0962-8819
Subject:
European Union; corn; genes; herbicide resistance; insects; markets; soybeans; Romania
Abstract:
... Romania was the third country in Europe and the tenth in the world, to commercially adopt genetically modified crops in 1999, only 3 years after they were first marketed globally. Half a million hectares of transgenic herbicide resistant soybean and insect resistant maize were grown in Romania during an uninterrupted 17-year period. After several years of continued declining area, the commercial c ...
DOI:
10.1007/s11248-018-0095-9

99. The interaction of two-spotted spider mites, Tetranychus urticae Koch, with Cry protein production and predation by Amblyseius andersoni (Chant) in Cry1Ac/Cry2Ab cotton and Cry1F maize

Author:
Yan-Yan Guo; Jun-Ce Tian; Wang-Peng Shi; Xue-Hui Dong; Jörg Romeis; Steven E. Naranjo; Richard L. Hellmich; Anthony M. Shelton
Source:
Transgenic research 2016 v.25 no.1 pp. 33-44
ISSN:
0962-8819
Subject:
Amblyseius andersoni; Bacillus thuringiensis; Lepidoptera; Tetranychus urticae; bacteria; biological control; corn; cotton; crops; crystal proteins; eggs; environmental assessment; fecundity; hatching; herbivores; insecticidal proteins; insects; integrated pest management; life history; mites; natural enemies; nontarget organisms; pests; predation; predators; rearing; risk assessment; survival rate; transgenic plants
Abstract:
... Crops producing insecticidal crystal (Cry) proteins from the bacterium, Bacillus thuringiensis (Bt), are an important tool for managing lepidopteran pests on cotton and maize. However, the effects of these Bt crops on non-target organisms, especially natural enemies that provide biological control services, are required to be addressed in an environmental risk assessment. Amblyseius andersoni (Aca ...
DOI:
10.1007/s11248-015-9917-1

100. The maize α-zein promoter can be utilized as a strong inducer of cellulase enzyme expression in maize kernels

Author:
Deborah Vicuna Requesens; Maria Elena Gonzalez Romero; Shivakumar P. Devaiah; Yeun-Kyung Chang; Ashley Flory; Stephen Streatfield; Rebecca Ring; Cassie Phillips; Nathan C. Hood; Cyrus Dean Marbaniang; John A. Howard; Elizabeth E. Hood
Source:
Transgenic research 2019 v.28 no.5-6 pp. 537-547
ISSN:
0962-8819
Subject:
Southern blotting; biomass; corn; endo-1,4-beta-glucanase; endosperm; genes; genetic engineering; globulins; glutelins; recombinant proteins; rice
Abstract:
... Expression of recombinant proteins in plants is a technology for producing vaccines, pharmaceuticals and industrial enzymes. For the past several years, we have produced recombinant proteins in maize kernels using only the embryo, primarily driving expression of foreign genes with the maize globulin-1 promoter. Although strong expression is obtained, these lines use only 10–12% of the seed tissue. ...
DOI:
10.1007/s11248-019-00162-1