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... Knowledge of the persistence of Cry proteins in transgenic corn residue after harvest is necessary to assess the ecological risk to nontarget organisms. The amount of protein remaining in crop residue declines over time by a combination of microbial decomposition and leaching, both influenced by temperature, precipitation, and the amount of residue-soil contact. Here, we investigated how long biol ...
... Ruminants and humans are unable to synthesize essential amino acids (EAAs) and conditionally essential amino acids (CEAAs) under normal conditions and need to acquire them from plant sources. Maize plays, as a major crop, a central role in global food security. However, maize is deficient in several EAAs and CEAAs. Genetic engineering has been successfully used to enrich the EAA content of maize t ...
bioactive properties; corn; genetic engineering; immunogenicity; insecticidal proteins; molecular weight; pests; plant proteins; protein sources; protein synthesis; safety assessment
Abstract:
... Safety assessment of genetically modified plants includes protein characterization to confirm the intended trait protein expression. In addition, to conduct safety tests, the large amount of purified protein needed is usually met through the use of a surrogate, microbially produced protein source. Characterization of the eCry3.1Ab and mCry3A proteins as derived from Event MZIR098 maize was challen ...
... Maize production is one of the most important activities for the Honduran economy, both in terms of area cultivated and food security provided. This article reports the results of a survey undertaken to gauge knowledge, perceptions, opinions, and attitudes of Honduran farmers towards genetically modified (GM) maize. Data were collected from 32 maize producers in 2018–19, of both conventional and G ...
Jerlie Mhay Matres; Julia Hilscher; Akash Datta; Victoria Armario-Nájera; Can Baysal; Wenshu He; Xin Huang; Changfu Zhu; Rana Valizadeh-Kamran; Kurniawan R. Trijatmiko; Teresa Capell; Paul Christou; Eva Stoger; Inez H. Slamet-Loedin
... Genome-editing technologies offer unprecedented opportunities for crop improvement with superior precision and speed. This review presents an analysis of the current state of genome editing in the major cereal crops- rice, maize, wheat and barley. Genome editing has been used to achieve important agronomic and quality traits in cereals. These include adaptive traits to mitigate the effects of clim ...
Deborah Vicuna Requesens; Maria Elena Gonzalez Romero; Shivakumar P. Devaiah; Yeun-Kyung Chang; Ashley Flory; Stephen Streatfield; Rebecca Ring; Cassie Phillips; Nathan C. Hood; Cyrus Dean Marbaniang; John A. Howard; Elizabeth E. Hood
... Expression of recombinant proteins in plants is a technology for producing vaccines, pharmaceuticals and industrial enzymes. For the past several years, we have produced recombinant proteins in maize kernels using only the embryo, primarily driving expression of foreign genes with the maize globulin-1 promoter. Although strong expression is obtained, these lines use only 10–12% of the seed tissue. ...
... A genetically modified (GM) commercial corn variety, MON810, resistant to European corn borer, has been shown to be non-toxic to mammals in a number of rodent feeding studies carried out in accordance with OECD Guidelines. Insect resistance results from expression of the Cry1Ab gene encoding an insecticidal Bt protein that causes lysis and cell death in susceptible insect larvae by binding to midg ...
Glycine max; Japan; canola; corn; cotton; data collection; environmental assessment; genetic engineering; information sources; risk; soybeans
Abstract:
... To date, there have been 160 regulatory approvals for environmental safety in Japan for the major genetically modified (GM) crops, including corn, soybean, canola and cotton. Confined field trials (CFTs) have been conducted in Japan for all single events, which contain various traits. The accumulated information from these previously conducted CFTs, as well as the agronomic field study data from o ...
... During the Green Revolution in the 1960s, breeding dwarf cultivars turned out to be a landmark, leading to a significant increase in the global production of wheat and rice. The most direct and effective strategy for breeding dwarf crops, among others, would be to control endogenous gibberellin (GA) levels of the crops. GA 2-oxidases are a group of 2-oxoglutarate-dependent dioxygenases that cataly ...
... The vitamin E family includes tocopherols and tocotrienols, which are essential lipid-soluble antioxidants necessary for human and livestock health. The seeds of many plant species, including maize, have high gamma (γ)-tocopherol but low alpha (α)-tocopherol contents; however, α-tocopherol is the most effective antioxidant. Therefore, it is necessary to optimize the tocopherol composition in plant ...
... Genetically modified crops undergo extensive evaluation to characterize their food, feed and environmental safety prior to commercial introduction, using a well-established, science-based assessment framework. One component of the safety assessment includes an evaluation of each introduced trait, including its source organism, for potential adverse pathogenic, toxic and allergenic effects. Several ...
European Union; corn; genes; herbicide resistance; insects; markets; soybeans; Romania
Abstract:
... Romania was the third country in Europe and the tenth in the world, to commercially adopt genetically modified crops in 1999, only 3 years after they were first marketed globally. Half a million hectares of transgenic herbicide resistant soybean and insect resistant maize were grown in Romania during an uninterrupted 17-year period. After several years of continued declining area, the commercial c ...
Bill Duncan; Elisa Leyva-Guerrero; Todd Werk; Duška Stojšin; Baltazar M. Baltazar; Silverio García-Lara; Mariana Zavala-López; Juan Manuel de la Fuente-Martínez; Chen Meng
... Genetically modified (GM) maize has been grown and safely consumed on a global scale since its commercialization in 1996. However, questions have been raised about the potential impact that GM maize could have on native maize landraces in Mexico, which is the center of origin and diversity of maize. This research was conducted to evaluate potential changes to maize landraces in an unlikely event o ...
Moon-Sub Lee; Eric K. Anderson; Duška Stojšin; Marc A. McPherson; Baltazar Baltazar; Michael J. Horak; Juan Manuel de la Fuente; Kunsheng Wu; James H. Crowley; A. Lane Rayburn; D. K. Lee
... Eastern gamagrass (Tripsacum dactyloides L.) belongs to the same tribe of the Poaceae family as maize (Zea mays L.) and grows naturally in the same region where maize is commercially produced in the USA. Although no evidence exists of gene flow from maize to eastern gamagrass in nature, experimental crosses between the two species were produced using specific techniques. As part of environmental r ...
... The large French research project GENIUS (2012–2019, https://www6.inra.genius-project_eng/) provides a good showcase of current genome editing techniques applied to crop plants. It addresses a large variety of agricultural species (rice, wheat, maize, tomato, potato, oilseed rape, poplar, apple and rose) together with some models (Arabidopsis, Brachypodium, Physcomitrella). Using targeted mutagene ...
... Genetically modified (GM) crops undergo large scale multi-location field trials to characterize agronomics, composition, and the concentration of newly expressed protein(s) [herein referred to as transgenic protein(s)]. The concentration of transgenic proteins in different plant tissues and across the developmental stages of the plant is considered in the safety assessment of GM crops. Reference o ...
Oscar Heredia Díaz; José Luis Aldaba Meza; Baltazar M. Baltazar; Germán Bojórquez Bojórquez; Luciano Castro Espinoza; José Luis Corrales Madrid; Juan Manuel de la Fuente Martínez; Héctor Abel Durán Pompa; José Alonso Escobedo; Armando Espinoza Banda; José Antonio Garzón Tiznado; Juvencio González García; José Luis Guzmán Rodríguez; Jesús Ignacio Madueño Martínez; José Luis Martínez Carrillo; Chen Meng; Francisco Javier Quiñones Pando; Enrique Rosales Robles; Ignacio Ruiz Hernández; José Elías Treviño Ramírez; Hugo Raúl Uribe Montes; Francisco Zavala García
... Environmental risk assessment (ERA) of genetically modified (GM) crops is a process to evaluate whether the biotechnology trait(s) in a GM crop may result in increased pest potential or harm to the environment. In this analysis, two GM insect-resistant (IR) herbicide-tolerant maize hybrids (MON-89Ø34-3 × MON-88Ø17-3 and MON-89Ø34-3 × MON-ØØ6Ø3-6) and one herbicide-tolerant GM hybrid (MON-ØØ6Ø3-6) ...
... Agrobacterium-mediated transformation is a complex process that is widely utilized for generating transgenic plants. However, one of the major concerns of this process is the frequent presence of undesirable T-DNA vector backbone sequences in the transgenic plants. To mitigate this deficiency, a ternary strain of A. tumefaciens was modified to increase the precision of T-DNA border nicking such th ...
Bacillus thuringiensis; community structure; corn; multidimensional scaling; pests; proteins; species diversity; toxins; transgenic plants; variance
Abstract:
... Non-lepidopteran pests are exposed to, and may be influenced by, Bt toxins when feeding on Bt maize that express insecticidal Cry proteins derived from Bacillus thuringiensis (Bt). In order to assess the potential effects of transgenic cry1Ie maize on non-lepidopteran pest species and ecological communities, a 2-year field study was conducted to compare the non-lepidopteran pest abundance, diversi ...
Jürgen Breitenbach; Marilise Nogueira; Gemma Farré; Changfu Zhu; Teresa Capell; Paul Christou; Gunther Fleck; Ulfert Focken; Paul D. Fraser; Gerhard Sandmann
Oncorhynchus mykiss; astaxanthin; color; corn; enantiomers; ethanol; feces; feed formulation; feed supplements; fish feeds; fish fillets; oils; pigmentation; raw materials; transgenic plants; trout; zeaxanthin
Abstract:
... Astaxanthin from a transgenic maize line was evaluated as feed supplement source conferring effective pigmentation of rainbow trout flesh. An extraction procedure using ethanol together with the addition of vegetal oil was established. This resulted in an oily astaxanthin preparation which was not sufficiently concentrated for direct application to the feed. Therefore, a concentration process invo ...