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You searched for: Journal name Transgenic research Remove constraint Journal name: Transgenic research Subject term corn Remove constraint Subject term: corn
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1. A specific real-time quantitative PCR detection system for event MON810 in maize YieldGard based on the 3'-transgene integration sequence

Author:
Hernandez, M.; Pla, M.; Esteve, T.; Prat, S.; Puigdomenech, P.; Ferrando, A.
Source:
Transgenic research 2003 v.12 no.2 pp. 179-189
ISSN:
0962-8819
Subject:
Zea mays; corn; transgenic plants; polymerase chain reaction; synthetic genes; Bacillus thuringiensis; bacterial insecticides; endotoxins; bacterial toxins; crystal proteins; insect control; plasmid vectors
PubMed:
12739886

2. Co-expression of a modified maize ribosome-inactivating protein and a rice basic chitinase gene in transgenic rice plants confers enhanced resistance to sheath blight

Author:
Kim, J.K.; Jang, I.C.; Wu, R.; Zuo, W.N.; Boston, R.S.; Lee, Y.H.; Ahn, I.P.; Nahm, B.H.
Source:
Transgenic research 2003 v.12 no.4 pp. 475-484
ISSN:
0962-8819
Subject:
rice; Oryza sativa; plant pathogenic fungi; Magnaporthe grisea; blight; genetic resistance; Bipolaris oryzae; fungal diseases of plants; Thanatephorus cucumeris; gene expression; protein synthesis inhibitors; disease resistance; gene segregation; Zea mays; corn; N-glycoside hydrolases; genetic transformation; biolistics; transgenes; chitinase
PubMed:
12885168

3. Functional analysis of two matrix attachment region (MAR) elements in transgenic maize plants

Author:
Sidorenko, L.; Bruce, W.; Maddock, S.; Tagliani, L.; Li, X.; Daniels, M.; Peterson, T.
Source:
Transgenic research 2003 v.12 no.2 pp. 137-154
ISSN:
0962-8819
Subject:
Zea mays; transgenes; transgenic plants; corn; nuclear matrix; gene expression; histochemistry; reporter genes; chromatin; promoter regions; beta-glucuronidase
PubMed:
12739882

4. Analysis of the maize polyubiquitin-1 promoter heat shock elements and generation of promoter variants with modified expression characteristics

Author:
Streatfield, S.J.; Magallanes-Lundback, M.E.; Beifuss, K.K.; Brooks, C.A.; Harkey, R.L.; Love, R.T.; Bray, J.; Howard, J.A.; Jilka, J.M.; Hood, E.E.
Source:
Transgenic research 2004 v.13 no.4 pp. 299-312
ISSN:
0962-8819
Subject:
Zea mays; corn; Zea diploperennis; promoter regions; mutation; ubiquitin; gene induction; heat stress; transgenic plants; transgenes; reporter genes; beta-glucuronidase; gene expression; seeds
DOI:
10.1023/B:TRAG.0000040053.23687.9c
PubMed:
15517990

5. Expression of cold-tolerant pyruvate, orthophosphate dikinase cDNA, and heterotetramer formation in transgenic maize plants

Author:
Ohta, S.; Ishida, Y.; Usami, S.
Source:
Transgenic research 2004 v.13 no.5 pp. 475-485
ISSN:
0962-8819
Subject:
genetic transformation; Zea mays; complementary DNA; antisense DNA; transgenic plants; Agrobacterium radiobacter; corn; gene expression; protein quaternary structure; phosphotransferases (kinases); cold tolerance; Flaveria
DOI:
10.1007/s11248-004-1452-4
PubMed:
15587271

6. Generation of marker-free transgenic maize by regular two-border Agrobacterium transformation vectors

Author:
Huang, S.; Gilbertson, L.A.; Adams, T.H.; Malloy, K.P.; Reisenbigler, E.K.; Birr, D.H.; Snyder, M.W.; Zhang, Q.; Luethy, M.H.
Source:
Transgenic research 2004 v.13 no.5 pp. 451-461
ISSN:
0962-8819
Subject:
genetic transformation; Zea mays; transgenic plants; Agrobacterium radiobacter; plasmid vectors; transfer DNA; corn; genetic markers
DOI:
10.1007/s11248-004-1453-3
PubMed:
15587269

7. Recombinant protein expression plasmids optimized for industrial E. coli fermentation and plant systems produce biologically active human insulin-like growth factor-1 in transgenic rice and tobacco plants

Author:
Panahi, M.; Alli, Z.; Cheng, X.; Belbaraka, L.; Belgoudi, J.; Sardana, R.; Phipps, J.; Altosaar, I.
Source:
Transgenic research 2004 v.13 no.3 pp. 245-259
ISSN:
0962-8819
Subject:
Oryza sativa; rice; Nicotiana tabacum; tobacco; transgenic plants; Escherichia coli; genetically engineered microorganisms; genetic transformation; plasmid vectors; transgenes; insulin-like growth factor I; humans; gene expression; recombinant proteins; genetic code; promoter regions; ubiquitin; Zea mays; corn; cell proliferation; neurons; cell lines
DOI:
10.1023/B:TRAG.0000034619.21613.d0
PubMed:
15359602

8. Assessing the transfer of genetically modified DNA from feed to animal tissues

Author:
Mazza, R.; Soave, M.; Morlacchini, M.; Piva, G.; Marocco, A.
Source:
Transgenic research 2005 v.14 no.5 pp. 775-784
ISSN:
0962-8819
Subject:
insecticidal proteins; pork; Zea mays; recombinant DNA; transgenes; feeds; swine feeding; transgenic plants; polymerase chain reaction; corn; delta-endotoxins; risk assessment; Bacillus thuringiensis; crystal proteins; zein; food safety; human food chain; animal tissues; traceability
DOI:
10.1007/s11248-005-0009-5
PubMed:
16245168

9. Effects of Bt maize on the herbivore Spodoptera littoralis (Lepidoptera: Noctuidae) and the parasitoid Cotesia marginiventris (Hymenoptera: Braconidae)

Author:
Vojtech, E.; Meissle, M.; Poppy, G.M.
Source:
Transgenic research 2005 v.14 no.2 pp. 133-144
ISSN:
0962-8819
Subject:
Zea mays; corn; transgenic plants; insect pests; Spodoptera littoralis; crop damage; tritrophic interactions; larvae; transgenes; gene expression; crystal proteins; insecticidal proteins; nontarget organisms; Cotesia marginiventris; parasitoids; predator-prey relationships; feeding preferences; bioassays; natural enemies; mortality; insect development
DOI:
10.1007/s11248-005-2736-z
PubMed:
16022385

10. Effects of Bt maize-fed prey on the generalist predator Poecilus cupreus L. (Coleoptera: Carabidae)

Author:
Meissle, M.; Vojtech, E; Poppy, G.M.
Source:
Transgenic research 2005 v.14 no.2 pp. 123-132
ISSN:
0962-8819
Subject:
Zea mays; corn; transgenic plants; insect pests; Spodoptera littoralis; crop damage; tritrophic interactions; transgenes; gene expression; crystal proteins; insecticidal proteins; nontarget organisms; Pterostichus cupreus; predatory insects; predator-prey relationships; feeding preferences; bioassays; pupae; larvae; mortality
DOI:
10.1007/s11248-004-6458-4
PubMed:
16022384

11. Evaluating the Induced-Odour Emission of a Bt Maize and its Attractiveness to Parasitic Wasps

Author:
Turlings, T.C.J.; Jeanbourquin, P.M.; Held, M.; Degen, T.
Source:
Transgenic research 2005 v.14 no.6 pp. 807-816
ISSN:
0962-8819
Subject:
Zea mays; corn; transgenic plants; gene expression; transgenes; Bacillus thuringiensis; insecticidal proteins; odors; odor emissions; volatile organic compounds; Cotesia marginiventris; Microplitis rufiventris; parasitoids; natural enemies; insect attractants; insect behavior; tritrophic interactions
DOI:
10.1007/s11248-005-0008-6
PubMed:
16315088

12. Assessment of Real-time PCR Based Methods for Quantification of Pollen-mediated Gene Flow from GM to Conventional Maize in a Field Study

Author:
Pla, M.; La Paz, J.L.; Peñas, G.; García, N.; Palaudelmàs, M.; Esteve, T.; Messeguer, J.; Melé, E.
Source:
Transgenic research 2006 v.15 no.2 pp. 219-228
ISSN:
0962-8819
Subject:
Zea mays; corn; transgenic plants; gene flow; pollen; pollen flow; polymerase chain reaction; introgression; risk assessment; cross pollination; transgenes; Bacillus thuringiensis; crystal proteins; insecticidal proteins; delta-endotoxins; Spain
DOI:
10.1007/s11248-005-4945-x
PubMed:
16604462

13. Expression of the Newcastle disease virus fusion protein in transgenic maize and immunological studies

Author:
Octavio Guerrero-Andrade; Elizabeth Loza-Rubio; Teresa Olivera-Flores; Tamás Fehérvári-Bone; Miguel Angel Gómez-Lim
Source:
Transgenic research 2006 v.15 no.4 pp. 455-463
ISSN:
0962-8819
Subject:
chickens; viral fusion proteins; seeds; antigens; transgenes; antibodies; transgenic plants; vaccines; corn; Western blotting; gene expression; Avian orthoavulavirus 1; genetic engineering; ubiquitin; hormones
Abstract:
... Transgenic plants have been employed successfully as a low-cost system for the production of therapeutically valuable proteins, including antibodies, antigens and hormones. Here, we report the expression of the fusion (F) gene of the Newcastle disease virus (NDV) in transgenic maize plants. The expression of the transgene, driven by the maize ubiquitin promoter, caused accumulation of the F protei ...
DOI:
10.1007/s11248-006-0017-0

14. Expression of the lipid transfer protein Ace-AMP1 in transgenic wheat enhances antifungal activity and defense responses

Author:
Subhankar Roy-Barman; Christof Sautter; Bharat B. Chattoo
Source:
Transgenic research 2006 v.15 no.4 pp. 435
ISSN:
0962-8819
Subject:
Allium cepa; Blumeria graminis f. sp. tritici; Tilletia indica; Triticum aestivum; Western blotting; antifungal properties; antimicrobial proteins; chitinase; corn; disease resistance; fungi; genetic engineering; glufosinate; inheritance (genetics); introns; leaves; lipid transfer proteins; phenylalanine ammonia-lyase; polymerase chain reaction; salicylic acid; systemic acquired resistance; transgenes; transgenic plants; ubiquitin; wheat
Abstract:
... To enhance fungal disease resistance, wheat plants (cv. Bobwhite) were engineered to constitutively express the potent antimicrobial protein Ace-AMP1 from Allium cepa, driven by a maize ubiquitin promoter along with its first intron. The bar gene was used for selection of putative transformants on medium containing phosphinothricin (PPT). Transgene inheritance, integration and stability of express ...
DOI:
10.1007/s11248-006-0016-1

15. Mycotoxin Reduction in Bt Corn: Potential Economic, Health, and Regulatory Impacts

Author:
Wu, F.
Source:
Transgenic research 2006 v.15 no.3 pp. 277-289
ISSN:
0962-8819
Subject:
imports; crystal proteins; plant pathogenic fungi; pest resistance; insecticidal proteins; genetic resistance; fungal diseases of plants; gene expression; Bacillus thuringiensis; international trade; disease resistance; economic impact; Zea mays; corn; aflatoxins; fumonisins; human food chain; transgenes; transgenic plants; food contamination
DOI:
10.1007/s11248-005-5237-1
PubMed:
16779644

16. Six Years after the Commercial Introduction of Bt Maize in Spain: Field Evaluation, Impact and Future Prospects

Author:
Eizaguirre, M.; Albajes, R.; Lopez, C.; Eras, J.; Lumbierres, B.; Pons, X.
Source:
Transgenic research 2006 v.15 no.1 pp. 1-12
ISSN:
0962-8819
Subject:
Zea mays; corn; transgenic plants; transgenes; Bacillus thuringiensis; insecticidal proteins; crystal proteins; gene expression; insecticide resistance; Sesamia nonagrioides; insect pests; defense mechanisms; larvae; dispersal behavior; field experimentation; nontarget organisms; environmental impact; predatory insects; Spain
DOI:
10.1007/s11248-005-3998-1
PubMed:
16475005

17. A leaf-based regeneration and transformation system for maize (Zea mays L.)

Author:
Ahmadabadi, Mohammad; Ruf, Stephanie; Bock, Ralph
Source:
Transgenic research 2007 v.16 no.4 pp. 437-448
ISSN:
0962-8819
Subject:
Zea mays; callus; callus culture; corn; food crops; genetic engineering; genetic transformation; genetically modified organisms; greenhouses; in vitro studies; leaves; tissue culture
Abstract:
... Efficient methods for in vitro propagation, regeneration, and transformation of plants are of pivotal importance to both basic and applied research. While being the world's major food crops, cereals are among the most difficult-to-handle plants in tissue culture which severely limits genetic engineering approaches. In maize, immature zygotic embryos provide the predominantly used material for esta ...
DOI:
10.1007/s11248-006-9046-y
PubMed:
17103238

18. A study of crop-to-crop gene flow using farm scale sites of fodder maize (Zea mays L.) in the UK

Author:
Rebecca Weekes; Theodore Allnutt; Caroline Boffey; Sarah Morgan; Mark Bilton; Roger Daniels; Christine Henry
Source:
Transgenic research 2007 v.16 no.2 pp. 203-211
ISSN:
0962-8819
Subject:
Zea mays; corn; crops; farms; forage; gene flow; genes; guidelines; herbicide resistance; invertebrates; probability; quantitative polymerase chain reaction; statistical analysis; statistical models; transgenic plants; weeds; United Kingdom
Abstract:
... From 2000 to 2003 a range of Farm Scale Evaluation (FSE) trials were established in the UK to assess the effect of the release and management of herbicide tolerant (HT) crops on arable weeds and invertebrates. The FSE trials for maize were also used to investigate crop-to-crop gene flow and to develop a statistical model for the prediction of gene flow frequency that can be used to evaluate curren ...
DOI:
10.1007/s11248-006-9036-0
PubMed:
17115253

19. A wheat genomic DNA fragment reduces pollen transmission of maize transgenes by reducing pollen viability

Author:
Scott, M. Paul; Peterson, Joan M.; Moran, Daniel L.; Sangtong, Varaporn; Smith, LaTrice
Source:
Transgenic research 2007 v.16 no.5 pp. 629-643
ISSN:
0962-8819
Subject:
viability; pollination; Zea mays; transgenes; transgenic plants; corn; Triticum aestivum; glufosinate; pollen germination; herbicide resistance; pollen
Abstract:
... A genomic DNA fragment from wheat carrying the Glu-1Dx5 gene has been shown to exhibit reduced pollen transmission in transgenic maize. To localize the region of the DNA fragment responsible for this reduced pollen transmission, we produced transgenic maize plants in which the wheat genomic DNA proximal to the 1Dx5 coding sequence was replaced with the maize 27 kDa γ-zein promoter. Like the wheat ...
Handle:
10113/3842
DOI:
10.1007/s11248-006-9055-x
PubMed:
17216545

20. Development of a tightly regulated and highly inducible ecdysone receptor gene switch for plants through the use of retinoid X receptor chimeras

Author:
Tavva, Venkata S.; Dinkins, Randy D.; Palli, Subba R.; Collins, Glenn B.
Source:
Transgenic research 2007 v.16 no.5 pp. 599-612
ISSN:
0962-8819
Subject:
Nicotiana tabacum; tobacco; Zea mays; corn; Glycine max; soybeans; protoplasts; transgenic plants; hormone receptors; transcription factors; humans; Locusta migratoria; Choristoneura fumiferana; recombinant proteins; gene expression; gene expression regulation; methoxyfenozide; reporter genes; luciferase; genetic transformation
Abstract:
... Chemical inducible gene regulation systems provide essential tools for the precise regulation of transgene expression in plants and animals. Recent development of a two-hybrid ecdysone receptor (EcR) gene regulation system has solved some of the drawbacks that were associated with the monopartate gene switch. To further improve the versatility of the two-hybrid EcR gene switch for wide spread use ...
Handle:
10113/3841
DOI:
10.1007/s11248-006-9054-y
PubMed:
17139530

21. Enhanced conversion of plant biomass into glucose using transgenic rice-produced endoglucanase for cellulosic ethanol

Author:
Oraby, Hesham; Venkatesh, Balan; Dale, Bruce; Ahmad, Rashid; Ransom, Callista; Oehmke, James; Sticklen, Mariam
Source:
Transgenic research 2007 v.16 no.6 pp. 739-749
ISSN:
0962-8819
Subject:
Acidothermus cellulolyticus; active sites; ammonia; bioethanol; biomass; burning; cellulose; corn; endo-1,4-beta-glucanase; food crops; gene expression; genes; genetically modified organisms; glucose; growth and development; hydrolysis; plant growth; proteins; rice; rice straw; sustainable technology; thermal stability
Abstract:
... The catalytic domain of Acidothermus cellulolyticus thermostable endoglucanase gene (encoding for endo-1,4-β-glucanase enzyme or E1) was constitutively expressed in rice. Molecular analyses of T1 plants confirmed presence and expression of the transgene. The amount of E1 enzyme accounted for up to 4.9% of the plant total soluble proteins, and its accumulation had no apparent deleterious effects on ...
DOI:
10.1007/s11248-006-9064-9
PubMed:
17237981

22. High level accumulation of α-glucan in maize kernels by expressing the gtfD gene from Streptococcus mutans

Author:
Zhang, Shirong; Dong, Jian G.; Wang, Tai; Guo, Sherry; Glassman, Kimberly; Ranch, Jerry; Nichols, Scott E.
Source:
Transgenic research 2007 v.16 no.4 pp. 467-478
ISSN:
0962-8819
Subject:
Streptococcus mutans; Zea mays; corn; crops; foods; glucose; glucosyltransferases; industrial applications; molecular weight; mutation; plant growth; polymerization; seeds; starch; sucrose; transgenes; transgenic plants
Abstract:
... Glucosyltransferases (GTFs, EC.2.4.1.5) are bacterial enzymes that catalyze the polymerization of glucose residues from sucrose, leading to the production of high molecular weight glucan with α-1,3 /α-1,6 linkages. Such glucans, with many potential food and industrial applications, do not normally exist in higher plants. We fused a mutant form of the gtfD gene from Sreptococcus mutans with the mai ...
DOI:
10.1007/s11248-006-9049-8

23. Definition and feasibility of isolation distances for transgenic maize cultivation

Author:
Olivier Sanvido; Franco Widmer; Michael Winzeler; Bernhard Streit; Erich Szerencsits; Franz Bigler
Source:
Transgenic research 2008 v.17 no.3 pp. 317-335
ISSN:
0962-8819
Subject:
European Union; aerial photography; agricultural land; corn; crop production; crops; geographic information systems; mixing; production technology; scientists; silage; transgenic plants; Europe
Abstract:
... A major concern related to the adoption of genetically modified (GM) crops in agricultural systems is the possibility of unwanted GM inputs into non-GM crop production systems. Given the increasing commercial cultivation of GM crops in the European Union (EU), there is an urgent need to define measures to prevent mixing of GM with non-GM products during crop production. Cross-fertilization is one ...
DOI:
10.1007/s11248-007-9103-1
PubMed:
17562214

24. Effect of promoter driving selectable marker on corn transformation

Author:
Shiva Prakash, N.; Prasad, V.; Chidambram, Thillai P.; Cherian, Shoba; Jayaprakash, T. L.; Dasgupta, Santanu; Wang, Qi; Mann, Michael T.; Spencer, T. Michael; Boddupalli, Raghava S.
Source:
Transgenic research 2008 v.17 no.4 pp. 695-704
ISSN:
0962-8819
Subject:
corn; genetic markers; genetically modified organisms; reporter genes; rice; transgenes
Abstract:
... Identification of an appropriate selection agent and its corresponding selectable marker gene is one of the first steps in establishing a transformation protocol for a given plant species. As the promoter controls expression level of the genes, the promoter driving the selectable marker gene can affect transformation. However, investigations into the direct effect of promoters driving selectable m ...
DOI:
10.1007/s11248-007-9149-0
PubMed:
17952623

25. Environmental impact of herbicide regimes used with genetically modified herbicide-resistant maize

Author:
Devos, Yann; Cougnon, Mathias; Vergucht, Sofie; Bulcke, Robert; Haesaert, Geert; Steurbaut, Walter; Reheul, Dirk
Source:
Transgenic research 2008 v.17 no.6 pp. 1059-1077
ISSN:
0962-8819
Subject:
European Union; Zea mays; active ingredients; acute toxicity; agricultural land; aquatic organisms; biodiversity; corn; crop production; crops; environmental impact; food loss; glyphosate; groundwater; herbicide resistance; human health; pesticide application; risk; transgenic plants; weed control; weeds; Belgium
Abstract:
... With the potential advent of genetically modified herbicide-resistant (GMHR) crops in the European Union, changes in patterns of herbicide use are predicted. Broad-spectrum, non-selective herbicides used with GMHR crops are expected to substitute for a set of currently used herbicides, which might alter the agro-environmental footprint from crop production. To test this hypothesis, the environment ...
DOI:
10.1007/s11248-008-9181-8
PubMed:
18404410

26. Expression of a bacterial α-amylase gene in transgenic rice seeds

Author:
Xu, Xiaoli; Fang, Jun; Wang, Wei; Guo, Jianli; Chen, Pinnan; Cheng, Jiaan; Shen, Zhicheng
Source:
Transgenic research 2008 v.17 no.4 pp. 645-650
ISSN:
0962-8819
Subject:
rice; sugars; seeds; Geobacillus stearothermophilus; corn; starch; pH; genes; temperature; alpha-amylase
Abstract:
... An α-amylase gene from Bacillus stearothermophilus under the control of the promoter of a major rice-seed storage protein was introduced into rice. The transgenic line with the highest α-amylase activity reached about 15,000 U/g of seeds (one unit is defined as the amount of enzyme that produces 1 μmol of reducing sugar in 1 min at 70°C). The enzyme produced in the seeds had an optimum pH of 5.0-5 ...
DOI:
10.1007/s11248-007-9144-5

27. Impact of genetic structures on haploid genome-based quantification of genetically modified DNA: theoretical considerations, experimental data in MON 810 maize kernels (Zea mays L.) and some practical applications

Author:
David Zhang; Aurélie Corlet; Stephane Fouilloux
Source:
Transgenic research 2008 v.17 no.3 pp. 393-402
ISSN:
0962-8819
Subject:
quantitative polymerase chain reaction; stakeholders; crop production; Zea mays; genetically modified organisms; haploidy; hybrids; loci; monitoring; equations; DNA; genome; males; corn; models; endosperm; homozygosity; females; prediction
Abstract:
... Real-time Polymerase Chain Reaction (PCR) based assays are widely used to estimate the content of genetically modified (GM) materials in food, feed and seed. It has been known that the genetic structures of the analyte can significantly influence the GM content expressed by the haploid genome (HG) % estimated using real-time PCR assays; this kind of influence is also understood as the impact of bi ...
DOI:
10.1007/s11248-007-9114-y

28. Plant development inhibitory genes in binary vector backbone improve quality event efficiency in soybean transformation

Author:
Xudong Ye; Edward J. Williams; Junjiang Shen; James A. Esser; Amy M. Nichols; Michael W. Petersen; Larry A. Gilbertson
Source:
Transgenic research 2008 v.17 no.5 pp. 827-838
ISSN:
0962-8819
Subject:
Phaseolus; corn; gene expression; levansucrase; phytoene synthase; plant development; soybeans; transgenes; transgenic plants; vector control
Abstract:
... Conventional Agrobacterium-mediated plant transformation often produces a significant frequency of transgenic events containing vector backbone sequence, which is generally undesirable for biotechnology applications. We tested methods to reduce the frequency of transgenic plants containing vector backbone by incorporating genes into the backbone that inhibit the development of transgenic plants. F ...
DOI:
10.1007/s11248-008-9169-4
PubMed:
18253857

29. Prey mediated effects of Bt maize on fitness and digestive physiology of the red spider mite predator Stethorus punctillum Weise (Coleoptera: Coccinellidae)

Author:
Fernando Álvarez-Alfageme; Natalie Ferry; Pedro Castañera; Felix Ortego; Angharad M. R. Gatehouse
Source:
Transgenic research 2008 v.17 no.5 pp. 943-954
ISSN:
0962-8819
Subject:
Bacillus thuringiensis; Stethorus punctillum; Tetranychus urticae; adulthood; cattle; corn; cysteine proteinases; digestion; fecundity; in vitro studies; insecticidal properties; trophic relationships; trypsin
Abstract:
... The present study investigated prey-mediated effects of two maize varieties expressing a truncated Cry1Ab, Compa CB (event Bt176) and DKC7565 (event MON810), on the biology of the ladybird Stethorus punctillum. Although immuno-assays demonstrated the presence of Cry1Ab in both prey and predator collected from commercial maize-growing fields, neither transgenic variety had any negative effects on s ...
DOI:
10.1007/s11248-008-9177-4
PubMed:
18322817

30. Transgenic maize endosperm containing a milk protein has improved amino acid balance

Author:
Bicar, Earl H.; Woodman-Clikeman, Wendy; Sangtong, Varaporn; Peterson, Joan M.; Yang, S. Samuel; Lee, Michael; Scott, M. Paul
Source:
Transgenic research 2008 v.17 no.1 pp. 59-71
ISSN:
0962-8819
Subject:
animal health; inheritance (genetics); siblings; lactalbumin; ears; lysine; humans; protein sources; corn; nutrient requirements; zein; loci; transgenes; transgenic plants; essential amino acids; nutritive value; endosperm; protein content; Sub-Saharan Africa
Abstract:
... In order to meet the protein nutrition needs of the world population, greater reliance on plant protein sources will become necessary. The amino acid balance of most plant protein sources does not match the nutritional requirements of monogastric animals, limiting their nutritional value. In cereals, the essential amino acid lysine is deficient. Maize is a major component of human and animal diets ...
DOI:
10.1007/s11248-007-9081-3
PubMed:
17387628

31. Transgenic maize plants expressing a fungal phytase gene

Author:
Chen, Rumei; Xue, Guangxing; Chen, Ping; Yao, Bin; Yang, Wenzhu; Ma, Qianli; Fan, Yunliu; Zhao, Zuoyu; Tarczynski, Mitchell C.; Shi, Jinrui
Source:
Transgenic research 2008 v.17 no.4 pp. 633-643
ISSN:
0962-8819
Subject:
Aspergillus niger; animal growth; animal manures; animal production; corn; diet; feed additives; feed processing; fermentation; gene overexpression; genes; glycosylation; hybrids; ingredients; phosphates; phosphorus; phytases; phytic acid; pollution; poultry; production costs; seeds; swine; transgenic plants; yeasts
Abstract:
... Maize seeds are the major ingredient of commercial pig and poultry feed. Phosphorus in maize seeds exists predominately in the form of phytate. Phytate phosphorus is not available to monogastric animals and phosphate supplementation is required for optimal animal growth. Undigested phytate in animal manure is considered a major source of phosphorus pollution to the environment from agricultural pr ...
DOI:
10.1007/s11248-007-9138-3
PubMed:
17932782

32. Visualisation of plastids in endosperm, pollen and roots of transgenic wheat expressing modified GFP fused to transit peptides from wheat SSU RubisCO, rice FtsZ and maize ferredoxin III proteins

Author:
Primavesi, Lucia F.; Wu, Huixia; Mudd, Elisabeth A.; Day, Anil; Jones, Huw D.
Source:
Transgenic research 2008 v.17 no.4 pp. 529-543
ISSN:
0962-8819
Subject:
actin; antibodies; chloroplasts; corn; endosperm; ferredoxins; fluorescence; leaves; peptides; pollen; protein synthesis; ribulose-bisphosphate carboxylase; rice; roots; tissues; transgenic plants; wheat
Abstract:
... The ability to target marker proteins to specific subcellular compartments is a powerful research tool to study the structure and development of organelles. Here transit sequences from nuclear-encoded, plastid proteins, namely rice FtsZ, maize non-photosynthetic ferredoxin III (FdIII) and the small subunit of RubisCO were used to target a modified synthetic GFP (S65G, S72A) to plastids. The locali ...
DOI:
10.1007/s11248-007-9126-7
PubMed:
17710559

33. Effect of volunteers on maize gene flow

Author:
Palaudelmàs, Montserrat; Peñas, Gisela; Melé, Enric; Serra, Joan; Salvia, Jordi; Pla, Maria; Nadal, Anna; Messeguer, Joaquima
Source:
Transgenic research 2009 v.18 no.4 pp. 583-594
ISSN:
0962-8819
Subject:
corn; gene flow; genetically modified organisms; pollen; temperate zones; volunteer plants
Abstract:
... Regulatory approvals for deliberate release of GM maize events into the environment have lead to real situations of coexistence between GM and non-GM, with some fields being cultivated with GM and conventional varieties in successive seasons. Given the common presence of volunteer plants in maize fields in temperate areas, we investigated the real impact of GM volunteers on the yield of 12 non-GM ...
DOI:
10.1007/s11248-009-9250-7
PubMed:
19225900

34. Enhanced single copy integration events in corn via particle bombardment using low quantities of DNA

Author:
Lowe, Brenda A.; Shiva Prakash, N.; Way, Melissa; Mann, Michael T.; Spencer, T. Michael; Boddupalli, Raghava S.
Source:
Transgenic research 2009 v.18 no.6 pp. 831-840
ISSN:
0962-8819
Subject:
corn; cultivars; gene expression; plasmids; screening; statistical analysis; transgenes
Abstract:
... Transgene copy number is an important criterion for determining the utility of transgenic events. Single copy integration events are highly desirable when the objective is to produce marker free plants through segregation or when it is necessary to introgress different transgenes into commercial cultivars from different transgenic events. In contrast multi-copy events are advocated by several auth ...
DOI:
10.1007/s11248-009-9265-0
PubMed:
19381853

35. Gene expression profiles of MON810 and comparable non-GM maize varieties cultured in the field are more similar than are those of conventional lines

Author:
Coll, Anna; Nadal, Anna; Collado, Rosa; Capellades, Gemma; Messeguer, Joaquima; Melé, Enric; Palaudelmàs, Montserrat; Pla, Maria
Source:
Transgenic research 2009 v.18 no.5 pp. 801-808
ISSN:
0962-8819
Subject:
corn; foods; gene expression; gene expression regulation; humans; leaves; microarray technology; transcriptome; transgenic plants
Abstract:
... Maize is a major food crop and genetically modified (GM) varieties represented 24% of the global production in 2007. Authorized GM organisms have been tested for human and environmental safety. We previously used microarrays to compare the transcriptome profiles of widely used commercial MON810 versus near-isogenic varieties and reported differential expression of a small set of sequences in leave ...
DOI:
10.1007/s11248-009-9266-z
PubMed:
19396622

36. High-level expression of active human alpha1-antitrypsin in transgenic tobacco chloroplasts

Author:
Nadai, M.; Bally, J.; Vitel, M.; Job, C.; Tissot, G.; Botterman, J.; Dubald, M.
Source:
Transgenic research 2009 v.18 no.2 pp. 173-183
ISSN:
0962-8819
Subject:
chloroplasts; corn; elastase; humans; leaves; proteinase inhibitors; proteins; soybeans; terminator regions; tobacco
Abstract:
... We have produced human alpha1-antitrypsin (A1AT), a major therapeutic protein, in genetically engineered tobacco plastids. Four different expression vectors have been evaluated which encode A1AT under the control of various 5' and 3' plastid expression elements. The use of heterologous promoter and terminator sequences derived from the corn and soybean plastid genomes leads to simpler and predicta ...
DOI:
10.1007/s11248-008-9209-0
PubMed:
18686007

37. Impact of 9 years of Bt-maize cultivation on the distribution of maize viruses

Author:
Achon, Maria Angeles; Alonso-Dueñas, Natalia
Source:
Transgenic research 2009 v.18 no.3 pp. 387-397
ISSN:
0962-8819
Subject:
Bacillus thuringiensis; Maize dwarf mosaic virus; Maize rough dwarf virus; Sugarcane mosaic virus; corn; field experimentation; genetic background; viruses
Abstract:
... This study assesses the effect of Bt-maize on the distribution of maize viruses. Random surveys were conducted in Spain between 2001 and 2006 to evaluate the occurrence of maize viruses in Bt-maize cultivation areas and in areas where this crop had not been introduced. Maize dwarf mosaic virus (MDMV) was the predominant virus in Bt-areas, and Maize rough dwarf virus (MRDV) was the most predominant ...
DOI:
10.1007/s11248-008-9231-2
PubMed:
19067216

38. Impact of Bt-corn MON88017 in comparison to three conventional lines on Trigonotylus caelestialium (Kirkaldy) (Heteroptera: Miridae) field densities

Author:
Rauschen, Stefan; Schultheis, Eva; Pagel-Wieder, Sibylle; Schuphan, Ingolf; Eber, Sabine
Source:
Transgenic research 2009 v.18 no.2 pp. 203-214
ISSN:
0962-8819
Subject:
Diabrotica virgifera virgifera; Ostrinia nubilalis; Trigonotylus caelestialium; corn; enzyme-linked immunosorbent assay; field experimentation; nontarget organisms; transgenic plants
Abstract:
... In Europe, Bt-corn resistant against the European Corn Borer has until now been the only genetically modified plant to be grown commercially. With the advent of the Western Corn Rootworm Bt-corn varieties with resistance against Coleoptera will become important. The cultivation of Bt-plants may have negative impacts on non-target organisms, i.e. all species not explicitly targeted by a given Bt-cr ...
DOI:
10.1007/s11248-008-9207-2
PubMed:
18668336

39. Ingestion and excretion of two transgenic Bt corn varieties by slugs

Author:
Zurbrügg, Corinne; Nentwig, Wolfgang
Source:
Transgenic research 2009 v.18 no.2 pp. 215-225
ISSN:
0962-8819
Subject:
Arion; Bacillus thuringiensis; Deroceras reticulatum; arthropods; beneficial organisms; corn; endotoxins; enzyme-linked immunosorbent assay; excretion; feces; food webs; ingestion; intestines; leaves; pests; predators; protein content; proteins; risk assessment; slugs; transgenic plants
Abstract:
... The release of transgenic Bacillus thuringiensis (Bt) corn expressing various Cry endotoxins has raised concern that these endotoxins are disseminated in the food web and may adversely affect non-target beneficial organisms, such as predators and organisms of the decomposer food web. We therefore investigated in a laboratory study, whether the Cry1Ab and Cry3Bb1 protein from Bt corn could potentia ...
DOI:
10.1007/s11248-008-9208-1
PubMed:
18763046

40. Oral immunogenicity and protective efficacy in mice of transgenic rice plants producing a vaccine candidate antigen (As16) of Ascaris suum fused with cholera toxin B subunit

Author:
Matsumoto, Yasunobu; Suzuki, Seiko; Nozoye, Tomoko; Yamakawa, Takashi; Takashima, Yasuhiro; Arakawa, Takeshi; Tsuji, Naotoshi; Takaiwa, Fumio; Hayashi, Yoshihiro
Source:
Transgenic research 2009 v.18 no.2 pp. 185-192
ISSN:
0962-8819
Subject:
rice; adjuvants; grain crops; blood serum; antigens; oral administration; Oryza sativa; antibodies; recombinant fusion proteins; Ascaris suum; corn; secondary immunization; transgenic plants; mice; vaccines; endosperm; immune response; cholera toxin; eggs
Abstract:
... Cereal crops such as maize and rice are considered attractive for vaccine production and oral delivery. Here, we evaluated the rice Oryza sativa for production of As16--an antigen protective against the roundworm Ascaris suum. The antigen was produced as a chimeric protein fused with cholera toxin B subunit (CTB), and its expression level in the endosperm reached 50 μg/g seed. Feeding the transgen ...
DOI:
10.1007/s11248-008-9205-4
PubMed:
18763047

41. Cloning and functional characterization of the maize carotenoid isomerase and β-carotene hydroxylase genes and their regulation during endosperm maturation

Author:
Li, Qunrui; Farre, Gemma; Naqvi, Shaista; Breitenbach, Jürgen; Sanahuja, Georgina; Bai, Chao; Sandmann, Gerhard; Capell, Teresa; Christou, Paul; Zhu, Changfu
Source:
Transgenic research 2010 v.19 no.6 pp. 1053-1068
ISSN:
0962-8819
Subject:
enzymes; beta-cryptoxanthin; genetically modified organisms; lycopene; zeaxanthin; gene expression; beta-carotene; introns; stop codon; cultivars; Zea mays; messenger RNA; complementary DNA; chromosomes; corn; mutation; lutein; inbred lines; biochemical pathways; endosperm
Abstract:
... In order to gain further insight into the partly-characterized carotenoid biosynthetic pathway in corn (Zea mays L.), we cloned cDNAs encoding the enzymes carotenoid isomerase (CRTISO) and β-carotene hydroxylase (BCH) using endosperm mRNA isolated from inbred line B73. For both enzymes, two distinct cDNAs were identified mapping to different chromosomes. The two crtiso cDNAs (Zmcrtiso1 and Zmcrtis ...
DOI:
10.1007/s11248-010-9381-x

42. Decomposition dynamics and structural plant components of genetically modified Bt maize leaves do not differ from leaves of conventional hybrids

Author:
Zurbrügg, Corinne; Hönemann, Linda; Meissle, Michael; Romeis, Jörg; Nentwig, Wolfgang
Source:
Transgenic research 2010 v.19 no.2 pp. 257-267
ISSN:
0962-8819
Subject:
Bacillus thuringiensis; adverse effects; carbon nitrogen ratio; cellulose; corn; field experimentation; growing season; hemicellulose; hybrids; leaves; plant residues; soil ecology
Abstract:
... The cultivation of genetically modified Bt maize has raised environmental concerns, as large amounts of plant residues remain in the field and may negatively impact the soil ecosystem. In a field experiment, decomposition of leaf residues from three genetically modified (two expressing the Cry1Ab, one the Cry3Bb1 protein) and six non-transgenic hybrids (the three corresponding non-transformed near ...
DOI:
10.1007/s11248-009-9304-x
PubMed:
19609704

43. Degradation of Cry1Ab protein from genetically modified maize (MON810) in relation to total dietary feed proteins in dairy cow digestion

Author:
Paul, Vijay; Guertler, Patrick; Wiedemann, Steffi; Meyer, Heinrich H. D.
Source:
Transgenic research 2010 v.19 no.4 pp. 683-689
ISSN:
0962-8819
Subject:
Simmental; abomasum; cecum; corn; crystal proteins; dairy cows; digesta; digestion; enzyme-linked immunosorbent assay; feces; insecticidal proteins; lactation; protein content; rumen; slaughter; small intestine; total mixed rations; transgenic plants
Abstract:
... To investigate the relative degradation and fragmentation pattern of the recombinant Cry1Ab protein from genetically modified (GM) maize MON810 throughout the gastrointestinal tract (GIT) of dairy cows, a 25 months GM maize feeding study was conducted on 36 lactating Bavarian Fleckvieh cows allocated into two groups (18 cows per group) fed diets containing either GM maize MON810 or nearly isogenic ...
DOI:
10.1007/s11248-009-9339-z
PubMed:
19888668
PubMed Central:
PMC2902738

44. Development of real time PCR assays for detection and quantification of transgene DNA of a Bacillus thuringiensis (Bt) corn hybrid in soil samples

Author:
Zhu, Bin; Ma, Bao-Luo; Blackshaw, Robert E.
Source:
Transgenic research 2010 v.19 no.5 pp. 765-774
ISSN:
0962-8819
Subject:
Bacillus thuringiensis; DNA; Pseudomonas stutzeri; Zea mays; antibiotic resistance; corn; crop rotation; field experimentation; growing season; horizontal gene transfer; hybrids; kanamycin; kanamycin kinase; quantitative polymerase chain reaction; risk; soil; soil sampling; soybeans; transgenes; transgenic plants
Abstract:
... Real time PCR assays were developed to detect and quantify the transgene DNA of a commercially released Bacillus thuringiensis (Bt) corn (Zea mays L.) hybrid (DKC42-23), which was derived from the event MON863 and also carried a neomycin phosphotransferase gene (the nptII gene). We applied the real time PCR assays to investigate the persistence of the transgene DNA in a field trial grown with DKC4 ...
DOI:
10.1007/s11248-009-9353-1
PubMed:
20044792

45. Occurrence and field densities of Coleoptera in the maize herb layer: implications for Environmental Risk Assessment of genetically modified Bt-maize

Author:
Rauschen, Stefan; Schaarschmidt, Frank; Gathmann, Achim
Source:
Transgenic research 2010 v.19 no.5 pp. 727-744
ISSN:
0962-8819
Subject:
Bacillus thuringiensis; Chrysomelidae; Coccinellidae; Lepidoptera; biological control agents; corn; environmental assessment; field experimentation; growing season; insects; laboratory experimentation; nontarget organisms; pests; risk assessment; statistical analysis; transgenic plants
Abstract:
... Beetles (Coleoptera) are a diverse and ecologically important group of insects in agricultural systems. The Environmental Risk Assessment (ERA) of genetically modified Bt-crop varieties with insect resistances thus needs to consider and assess the potential negative impacts on non-target organisms belonging to this group. We analysed data gathered during 6 years of field-release experiments on the ...
DOI:
10.1007/s11248-009-9351-3
PubMed:
20012775

46. A semi-quantitative approach to GMO risk-benefit analysis

Author:
E. Jane Morris
Source:
Transgenic research 2011 v.20 no.5 pp. 1055-1071
ISSN:
0962-8819
Subject:
biosafety; case studies; corn; decision support systems; developing countries; genetically modified organisms; insects; laws and regulations; precautionary principle; risk; risk-benefit analysis; South Africa
Abstract:
... In many countries there are increasing calls for the benefits of genetically modified organisms (GMOs) to be considered as well as the risks, and for a risk-benefit analysis to form an integral part of GMO regulatory frameworks. This trend represents a shift away from the strict emphasis on risks, which is encapsulated in the Precautionary Principle that forms the basis for the Cartagena Protocol ...
DOI:
10.1007/s11248-010-9480-8
PubMed:
21197601

47. Laboratory toxicity studies demonstrate no adverse effects of Cry1Ab and Cry3Bb1 to larvae of Adalia bipunctata (Coleoptera: Coccinellidae): the importance of study design

Author:
Álvarez-Alfageme, Fernando; Bigler, Franz; Romeis, Jörg
Source:
Transgenic research 2011 v.20 no.3 pp. 467-479
ISSN:
0962-8819
Subject:
Adalia bipunctata; Bacillus thuringiensis; Tetranychidae; adverse effects; corn; experimental design; exposure pathways; ingestion; larvae; mortality; proteins; sucrose; toxicity; toxicity testing; transgenic plants
Abstract:
... Scientific studies are frequently used to support policy decisions related to transgenic crops. Schmidt et al., Arch Environ Contam Toxicol 56:221-228 (2009) recently reported that Cry1Ab and Cry3Bb were toxic to larvae of Adalia bipunctata in direct feeding studies. This study was quoted, among others, to justify the ban of Bt maize (MON 810) in Germany. The study has subsequently been criticized ...
DOI:
10.1007/s11248-010-9430-5
PubMed:
20740377
PubMed Central:
PMC3090567

48. Non-target organism effects tests on Vip3A and their application to the ecological risk assessment for cultivation of MIR162 maize

Author:
Raybould, Alan; Vlachos, Demetra
Source:
Transgenic research 2011 v.20 no.3 pp. 599-611
ISSN:
0962-8819
Subject:
Bacillus thuringiensis; Daphnia magna; Lepidoptera; adverse effects; biological control; corn; crop yield; crops; evolution; insecticidal proteins; mechanism of action; nontarget organisms; pest resistance; pesticide application; pests; risk assessment; transgenic plants
Abstract:
... Transgenic crops producing insecticidal proteins from Bacillus thuringiensis (Bt) provide economic, environmental and health benefits by maintaining or increasing crop yields with fewer applications of insecticide. To sustain these benefits, it is important to delay the evolution of insect resistance to the proteins, and to ensure that the proteins do not harm non-target organisms, particularly th ...
DOI:
10.1007/s11248-010-9442-1
PubMed:
20839052

49. Simultaneous expression of Arabidopsis ρ-hydroxyphenylpyruvate dioxygenase and MPBQ methyltransferase in transgenic corn kernels triples the tocopherol content

Author:
Naqvi, Shaista; Farré, Gemma; Zhu, Changfu; Sandmann, Gerhard; Capell, Teresa; Christou, Paul
Source:
Transgenic research 2011 v.20 no.1 pp. 177-181
ISSN:
0962-8819
Subject:
Arabidopsis; biochemical pathways; biofortification; clones; complementary DNA; corn; gamma-tocopherol; gene expression; human health; isomers; metabolic engineering; methyltransferases; seeds; substrate specificity; transgenic plants
Abstract:
... The quantity and composition of tocopherols (compounds with vitamin E activity) vary widely among different plant species reflecting the expression, activity and substrate specificity of enzymes in the corresponding metabolic pathway. Two Arabidopsis cDNA clones corresponding to ρ-hydroxyphenylpyruvate dioxygenase (HPPD) and 2-methyl-6-phytylplastoquinol methyltransferase (MPBQ MT) were constituti ...
DOI:
10.1007/s11248-010-9393-6

50. Assessing the ecological risks from the persistence and spread of feral populations of insect-resistant transgenic maize

Author:
Alan Raybould; Laura S. Higgins; Michael J. Horak; Raymond J. Layton; Nicholas P. Storer; Juan Manuel De La Fuente; Rod A. Herman
Source:
Transgenic research 2012 v.21 no.3 pp. 655-664
ISSN:
0962-8819
Subject:
comparative risk assessment; corn; crops; field experimentation; risk communication; risk perception; transgenic plants
Abstract:
... One source of potential harm from the cultivation of transgenic crops is their dispersal, persistence and spread in non-agricultural land. Ecological damage may result from such spread if the abundance of valued species is reduced. The ability of a plant to spread in non-agricultural habitats is called its invasiveness potential. The risks posed by the invasiveness potential of transgenic crops ar ...
DOI:
10.1007/s11248-011-9560-4
PubMed:
22002083
PubMed Central:
PMC3348485